Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| [characteristics of viral infections in patients with chronic obstructive bronchitis]. | virological and immunological studies on 53 patients with chronic obstructive bronchitis at the clinical stage of fading exacerbation were conducted. a high percentage of active viral infection (43%), persistence of viruses (25%) and virus-specific components (ribonucleoprotein in influenza) in the cells of brush biopsy bronchial mucosa specimens was characteristic for these patients. the respiratory-syncytial virus was the most common persisting virus (11%). a high percentage of association (72 ... | 1987 | 3118490 |
| expression and glycosylation of the respiratory syncytial virus g protein in saccharomyces cerevisiae. | a cdna encoding the entire amino acid sequence of the g glycoprotein of respiratory syncytial virus (rsv) was inserted into a yeast-escherichia coli shuttle vector such that expression of the virus gene was regulated by the yeast gal1 promoter. transformation of saccharomyces cerevisiae with the vector led to the formation of the g protein when cells were grown in the presence of galactose. under these conditions the rsv g appeared as a 60- to 65-kda glycosylated protein. expression of the g cdn ... | 1987 | 3113070 |
| respiratory syncytial virus infection in hospitalized children over one year of age. | 1986 | 3110028 | |
| specific human cytotoxic t cells recognize b-cell lines persistently infected with respiratory syncytial virus. | the t-lymphocyte response to respiratory syncytial (rs) virus has been invoked to explain the bronchiolitis and pneumonia caused by rs virus in human infants. however, t cells also appear to play a role in protection against rs virus infection. although rs virus-specific human lymphocytes have been demonstrated, neither the phenotype nor the function of the lymphocytes was characterized. we describe here the induction of anti-rs virus cytotoxic t lymphocytes, in both bulk culture and restimulate ... | 1986 | 3097646 |
| evaluation of a commercially available direct immunofluorescent staining reagent for the detection of respiratory syncytial virus in respiratory secretions. | a commercially-available direct immunofluorescence (if) reagent (imagen; boots-celltech, slough, berkshire, united kingdom) was similar in sensitivity and specificity to the conventional indirect if test for the detection of respiratory syncytial virus in respiratory secretions. both if tests were more sensitive than culture, particularly for specimens transported from outside the institution. | 1986 | 3088032 |
| respiratory syncytial virus--oklahoma. | 1986 | 3081783 | |
| [comparison between immunofluorescence and immunoenzymatic assay for the rapid diagnosis of the respiratory syncytial virus in nasopharyngeal secretions]. | an enzyme immunoassay, rsv-eia abbot, was evaluated by comparison with indirect immunofluorescence. nasopharyngeal secretions obtained from 95 infants and young children with acute respiratory infections were examined for the presence of respiratory syncytial virus antigens with both methods. specimens were stored at -70 degrees c before being tested by eia. out of 60 samples positive by indirect immunofluorescence, 46 were also positive by rsv-eia (sensitivity 78.7%) and 34 out of 35 immunofluo ... | 1988 | 3073406 |
| evaluation in non-human primates of the safety, immunogenicity and efficacy of recombinant vaccinia viruses expressing the f or g glycoprotein of respiratory syncytial virus. | it has been shown previously that immunization with recombinant vaccinia viruses expressing the f or g envelope glycoprotein of human respiratory syncytial virus (rsv) strain a2 induced a protective immune response in the lower respiratory tract of cotton rats against live rsv challenge. as a continuation of these studies, the safety, immunogenicity and efficacy of these recombinant vaccinia viruses was evaluated in non-human primates. rhesus and patas monkeys were each inoculated intradermally ... | 1988 | 3072795 |
| [bronchiolitis caused by respiratory syncytial virus in very young infants and transmitted maternal antibodies]. | the study with an enzyme linked immunosorbent assay of the sera of 57 infants aged less than 9 weeks and developing a bronchiolitis, argues for the absence of a protective role for the maternally transmitted anti-respiratory syncytial virus (rsv) igg and for the existence of a positive relationship between these antibodies levels - which are not neutralizing - and the severity of rsv bronchiolitis. | 1988 | 3071286 |
| can we predict or prevent sudden unexpected deaths during infancy? | mechanisms for sudden unexpected infant deaths remain unproven but the possibilities include cardiac arrhythmias, hypoglycaemia, prolonged apnoea from central respiratory control failure, seizure-induced apnoea, prolonged airway obstruction and alveolar atelectasis. many of these mechanisms could be treated if the infant at risk could be identified. to date, identification programmes have not been sufficiently sensitive or specific. all the evidence presently available suggests that clinical and ... | 1988 | 3068658 |
| time-resolved fluoroimmunoassay compared with virus isolation for rapid detection of respiratory syncytial virus in nasopharyngeal aspirates. | two monoclonal antibodies against two distinct conserved epitopes of the respiratory syncytial virus (rsv) nucleocapsid protein were used in a direct time-resolved fluoroimmunoassay (tr-fia) for the detection of rsv antigens in nasopharyngeal aspirates. the capture antibody was adsorbed to the solid phase of microdilution strip wells, and the indicator antibody was labeled with a europium chelate. specimens and label were incubated simultaneously for 1 h at 37 degrees c in the coated wells. afte ... | 1988 | 3068251 |
| vitamin a status of children with a history of respiratory syncytial virus infection in infancy. | in order to extend our earlier observation that children who experience frequent respiratory episodes may benefit from vitamin a supplementation, 206 children aged 2-7 years who had been hospitalized for bronchiolitis during infancy were randomized into a controlled trial of vitamin a supplementation. of these, 149 met the criteria of protocol compliance after 12 months of follow-up. mean plasma retinol at baseline was 39.2 micrograms/100 ml (s.e.m. = 1.0) and did not increase after 12 months (m ... | 1988 | 3067695 |
| respiratory syncytial virus. | rsv is now understood to be the most significant viral respiratory pathogen of infants and is capable of causing both bronchiolitis and pneumonia. it is a particular risk to hospitalized infants as the virus is easily spread through close contact. the most vulnerable infants are those who suffer with either congenital heart disease or bronchopulmonary dysplasia who easily fall prey to pulmonary complications of infection. strict environmental control and the use of protective clothing and eyewea ... | 1988 | 3066824 |
| the effect of common bovine respiratory diseases on tidal breathing flow-volume loops. | in order to better understand the bovine breathing pattern, tidal breathing flow-volume loops (tbfvl) were analyzed in 24 healthy cattle of different body weights (range: 37-660 kg) (group a) and in 28 cattle suffering from the common respiratory diseases: verminous bronchitis (group b); shipping fever (group c); acute respiratory distress syndrome (group d); respiratory syncytial virus pneumonia (group e); organophosphate poisoning (group f); and necrotic laryngitis (group g). respiratory airfl ... | 1988 | 3066008 |
| [the status of research to develop new vaccines against infection caused by the respiratory syncytial virus]. | 1988 | 3064426 | |
| detection, pathogenesis, and therapy of respiratory syncytial virus infections. | respiratory syncytial virus (rsv) infection is a major cause of serious lower respiratory disease in infancy and early childhood. the unique pathogenesis of lower respiratory illness due to rsv offers some intriguing clues to the role of the human immune system in both protection against and development of respiratory illness. more than any other virus, rapid diagnostic techniques have been especially successful in identifying rsv infection. many of these techniques could be easily adaptable to ... | 1988 | 3060243 |
| diagnosis of respiratory syncytial virus infection. | 1988 | 3054781 | |
| rapid diagnosis of infections caused by respiratory syncytial virus. | 1988 | 3053459 | |
| comparison of the abbott and ortho enzyme immunoassays and cell culture for the detection of respiratory syncytial virus in nasopharyngeal specimens. | a comparison of the abbott laboratories and the ortho diagnostic systems respiratory syncytial virus (rsv) enzyme immunoassays (eia) and hep-2 cell culture for the detection of rsv in 81 nasopharyngeal (np) specimens from pediatric patients with lower respiratory tract infection was carried out. the sensitivity and specificity of the abbott test compared to confirmed infection was 92.3% and 100.0%, respectively. the sensitivity and specificity of the ortho test was 87.5% and 80.3%, respectively. ... | 1988 | 3053011 |
| passive transfer of respiratory syncytial virus (rsv) antiserum suppresses the immune response to the rsv fusion (f) and large (g) glycoproteins expressed by recombinant vaccinia viruses. | in young infants who possess maternally derived respiratory syncytial virus (rsv) antibodies, the antibody response to rsv glycoproteins is relatively poor, despite extensive replication of rsv. in the present study, it was found that cotton rat rsv hyperimmune antiserum suppressed the antibody response to the rsv glycoproteins but not the response to vaccinia virus antigens when the antiserum was passively transferred to cotton rats prior to infection with vaccinia recombinant viruses expressin ... | 1988 | 3047432 |
| ribavirin in respiratory syncytial virus infection. a double blind placebo controlled trial is needed. | 1988 | 3046516 | |
| bovine respiratory syncytial virus-specific monoclonal antibodies. | five hybridomas were produced which secreted monoclonal antibodies to bovine respiratory syncytial virus (brsv). two antibodies (8g12, 15c7) neutralized the virus and inhibited syncytia formation in vitro. these monoclonal antibodies also stained, by indirect fluorescent assay, an external envelope protein of living virus-infected cells, and recognized the 48k subunit of the viral fusion protein by western blot analysis of bovine respiratory syncytial virus-infected cell lysates. three other mon ... | 1988 | 3046116 |
| respiratory syncytial virus: heterogeneity of subgroup b strains. | in order to investigate further possible structural differences among the two subgroups of respiratory syncytial virus (rsv), we analysed the antigenic characteristics and size of structural proteins of 20 subgroup a and 43 subgroup b strains by their reactions with monoclonal antibodies (mabs) directed against the proteins of rsv using immunofluorescence, elisa and radioimmunoprecipitation assays. the latter test also enabled determination of the size of different structural components. the 37 ... | 1988 | 3045252 |
| respiratory syncytial virus pneumonia in a cardiac transplant recipient. | 1988 | 3045218 | |
| non-neoplastic pulmonary multinucleate giant cells. comparative study of multinucleate giant cells in porcine dermatosis vegetans and other polykaryocytic conditions. | pulmonary multinucleate giant cells (mgcs) in porcine dermatosis vegetans (dv) were compared with virus-induced and inflammatory-associated mgcs, with particular attention to mechanisms involved in the formation of such cells. ultrastructurally, pigs with dv showed numerous pulmonary mgcs with transversely-sectioned interdigitating microvillous structures. microvilli were observed closely apposed to type-ii pneumocytes and with morphological characteristics of membrane bridge formation. rabbits ... | 1988 | 3044400 |
| respiratory syncytial virus infections and intravenous gamma-globulins. | the respiratory syncytial virus (rsv) is a common cause of bronchiolitis and pneumonia in infants and young children. throughout the world annual rsv epidemics result in numerous hospitalizations, substantial morbidity and some mortality. until the recent introduction of ribavirin only supportive therapy has been available for treating these infections. the development of animal models of rsv infection and the observation that some lots of immunoglobulin prepared for intravenous administration c ... | 1988 | 3041354 |
| pneumonias in adults due to mycoplasma, chlamydiae, and viruses. | pneumonias in adults due to mycoplasma, chlamydiae, and viruses are a common clinical problem. these microorganisms contribute to the etiologies in 6-35% of all cases of pneumonia and are the sole pathogens in 1-17% of hospitalized cases. important trends and developments in the field include the emergence of a chlamydia psittaci strain (twar) that is passaged from human to human, causes a mycoplasma-like illness, and that is relatively resistant to erythromycin, the recognition of respiratory s ... | 1987 | 3037901 |
| epstein-barr virus, cytomegalovirus, and other viral infections in children after liver transplantation. | we studied 51 consecutive pediatric patients for the frequency and morbidity of viral infections after liver transplantation. the incidence of primary (67%) and reactivation (48%) epstein-barr virus (ebv) infections and reactivation (88%) cytomegalovirus (cmv) infection was comparable to that seen in adult transplant recipients. however, fewer pediatric than adult transplant recipients experienced primary cmv infection (p less than .01). five (38%) of 13 cmv infections were symptomatic and inclu ... | 1987 | 3036964 |
| respiratory virus infection of peripheral blood monocytes: correlation with ageing of cells and interferon production in vitro. | the ability of respiratory syncytial virus (rsv) and parainfluenza virus type 3 (piv3) to replicate in peripheral blood monocytes cultured in vitro for 1, 2, 4 or 7 days prior to infection was investigated. inoculation of 1-day old monocytes produced at least tenfold less new virus than infection of the older, more macrophage-like cells for both viruses. piv3 induced extensive syncytium formation, whereas rsv caused a cytopathic effect manifest by increased rounding of the cells with minimal syn ... | 1987 | 3035067 |
| preparation of nasopharyngeal secretions for immunofluorescence by one-step centrifugation through percoll. | a simple method was developed for separation of cells from nasopharyngeal secretion for the diagnosis of respiratory virus infections by immunofluorescence microscopy. the diluted specimen, containing dithiothreitol to break up the mucus, was centrifuged once through a cushion of 20% percoll (colloidal silica, a density gradient medium), which permitted sedimentation of cells through the cushion, but retained mucus on top of it. the pelleted cells were resuspended, and microscope slides were the ... | 1987 | 3034944 |
| seroepidemiological studies on the occurrence of common respiratory infections in paediatric student nurses and medical technology students. | the occupational risk of acquiring minor respiratory infections for paediatric student nurses was estimated by performing serological examinations with influenza a, b, c, parainfluenza, mumps, respiratory syncytial virus, adenovirus and mycoplasma pneumoniae at 6-month intervals over a period of 4 years in paediatric student nurses at two schools of nursing and students at one school of medical technology. titre increases against all tested agents occurred 1.86 times more often in the student nu ... | 1987 | 3030790 |
| molecular cloning and sequence analysis of the human parainfluenza 3 virus gene encoding the matrix protein. | the sequence of the matrix (m) protein gene and contiguous intergenic regions of the human parainfluenza 3 virus (pf3) was determined by molecular cloning. the encoded m protein contains 354 amino acids and has a predicted mol wt of 39,506. the m protein amino acid sequence was compared to the homologous proteins from other members of the paramyxoviridae family. the pf3 protein shared 61% homology with the sendai virus protein and approximately 35% homology with measles and canine distemper viru ... | 1987 | 3029963 |
| diseases of wapiti utilizing cattle range in southwestern alberta. | specimens from 28 wapiti (cervus elaphus canadensis) were collected by hunters in southwestern alberta in 1984. various tests were performed to detect infections and conditions that could affect cattle sharing the range or cause disease in wapiti. serum antibodies were present against leptospiral serovars autumnalis (25%), bratislava (4%), and icterohaemorrhagiae (8%), and the viruses of bovine virus diarrhea (52%), infectious bovine rhinotracheitis (45%), and parainfluenza type 3 (13%). no sero ... | 1987 | 3029443 |
| efficiency of immunofluorescence for rapid detection of common respiratory viruses. | rapid immunofluorescence (fa) methods for the detection of common respiratory viruses were compared with culture results over a 3-year period to assess the relative efficiency of fa in a clinical laboratory setting. for respiratory syncytial virus, efficiencies were high (sensitivity, 90 to 95%; specificity, 92 to 95%). the sensitivity of fa for detection of parainfluenza virus type 1, parainfluenza virus type 3, influenza a virus, and adenoviruses ranged from 28 to 63%, but specificities for th ... | 1987 | 3029168 |
| [rhinovirus and acute respiratory infections in infants]. | human leucocytary interferon (ifn) in a concentration of 2,000 ul/ml stops the development of 9 rhinovirus (rv) strains. the use of serum ifn antibodies (saif) reverses this effect. of 256 nasal aspirates culture negative for other respiratory viruses, 40 rv were isolated with saif as opposed to 19 without serum anti ifn. the frequency and some clinical aspects are emphasized in infants: 43% had bronchiolitis similar to respiratory syncytial virus (rsv) infections and atopy was frequent. | 1986 | 3028312 |
| immunofluorescence in the serological diagnosis of parainfluenza type 3 and respiratory syncytial virus infection in calves. | the fluorescent antibody (fa) test is compared with the haemagglutination inhibition (hi) test for parainfluenza virus type 3 (pi-3) and virus neutralisation (vn) test for respiratory syncytial (rs) virus for detection and titration of virus-specific antibodies. in experimentally inoculated calves pi-3 and rs virus fa tests detected seroconversion at the same time as hi and vn tests, however, in serially diluted sera, the fa test was positive to higher dilution. in studies with paired samples fr ... | 1986 | 3027802 |
| infant pneumonitis associated with cytomegalovirus, chlamydia, pneumocystis, and ureaplasma: follow-up. | a total of 205 infants who were hospitalized when younger than 3 months of age for pneumonitis were followed longitudinally. of these patients, 145 (70%) had evidence of infection with one or more pathogens. the most common etiologic agents were chlamydia trachomatis 61/193 (36%), respiratory syncytial virus 33/142 (23%), cytomegalovirus 42/203 (20%), pneumocystis carinii 30/171 (17%), and ureaplasma urealyticum 21/125 (16%). the initial clinical presentation was characterized by cough, rales, n ... | 1987 | 3025807 |
| nucleotide sequence of the gene encoding the fusion glycoprotein of newcastle disease virus. | the nucleotide sequence of the gene encoding the fusion (f) glycoprotein of the beaudette c strain of newcastle disease virus (ndv) has been determined from cdna clones obtained from virion rna. the gene is 1792 nucleotides long, including mrna start and polyadenylation signals typical of paramyxoviruses. the single open reading frame encodes a polypeptide of 553 amino acids, with a predicted molecular weight of 59042. the f polypeptide has three regions of high hydrophobicity: an n-terminal sig ... | 1986 | 3025345 |
| clinical observations on mechanical ventilation for respiratory failure in bronchiolitis. | an unusually large number of infants (82) were admitted to stanford university hospital from november 1, 1983, through may 31, 1985, with a diagnosis of bronchiolitis requiring oxygen therapy. a larger percentage of these infants (17/82 = 21%) than generally expected required mechanical ventilation for respiratory failure. fourteen infants had respiratory syncytial virus (rsv) infections, and three had parainfluenza virus infections. ten patients had respiratory difficulties as neonates. the mec ... | 1986 | 3022224 |
| in vitro effect of ascorbic acid on infectivity of herpesviruses and paramyxoviruses. | suspensions of herpes simplex virus types 1 and 2, cytomegalovirus, and parainfluenzavirus type 2 were inactivated within 24 h when treated at 37 degrees c with 1 mg (5.05 mm) of copper-catalyzed sodium ascorbate per ml. the infectivity titer of respiratory syncytial virus was reduced substantially after 24 h but required 48 h for inactivation. under these conditions, inactivation of these viruses was also successfully achieved with 5.68 mm catalyzed ascorbic acid. copper (cu2+), when added with ... | 1986 | 3021808 |
| ribavirin: a clinical overview. | ribavirin, a broad spectrum, non-interferon-inducing virustatic chemotherapeutic agent, demonstrates activity against a wide range of rna and dna viruses, including the retrovirus known to cause the acquired immune deficiency syndrome. the drug's proposed mechanism of action, as well as pharmacokinetics are discussed, and preclinical toxicity, safety and clinical efficacy studies are presented. to date, the best success has occurred in the use of ribavirin to treat respiratory syncytial virus in ... | 1986 | 3021519 |
| fever in respiratory virus infections. | the case records of 258 children with adenovirus; influenza a or b virus; parainfluenza 1, 2, or 3 virus; or respiratory syncytial virus infections were studied retrospectively with special attention to the degree and duration of fever. a temperature of 39.0 degrees c or higher was most frequently recorded in adenovirus, influenza a, and influenza b virus infections (in 68%, 84%, and 65%, respectively). the mean highest degree of fever in respiratory virus infections (39.2 degrees c +/- 0.6 degr ... | 1986 | 3020965 |
| the association between serological titers in infectious bovine rhinotracheitis virus, bovine virus diarrhea virus, parainfluenza-3 virus, respiratory syncytial virus and treatment for respiratory disease in ontario feedlot calves. | a seroepidemiological study of the association between antibody titers to infectious bovine rhinotracheitis, parainfluenza-3, bovine virus diarrhea and bovine respiratory syncytial viruses, and treatment for bovine respiratory disease was conducted. a total of 322 calves from five different groups were bled on arrival, then one month later all cases (cattle treated for bovine respiratory disease) were rebled together with an equal number of controls (cattle not treated for any disease). titers t ... | 1986 | 3017529 |
| fusion glycoprotein of human parainfluenza virus type 3: nucleotide sequence of the gene, direct identification of the cleavage-activation site, and comparison with other paramyxoviruses. | the complete sequences of the fusion (f) mrna and protein of human parainfluenza virus type 3 (pf3) were determined from overlapping cdna clones. to confirm the cdna sequence, the complete sequence of the f gene was determined independently by dideoxynucleotide sequencing of genomic rna using synthetic oligonucleotide primers. the mrna contains 1845 nucleotides, exclusive of poly (a), has an unusually long (193-nucleotide) 5' nontranslated region, and encodes an f0 protein of 539 amino acids. th ... | 1986 | 3012869 |
| patterns of viral respiratory tract infections in singapore. | a second retrospective study on viral respiratory tract infections, based on 12 years' laboratory reports for the influenza viruses, and 9 years' reports for the other respiratory viruses was made. seasonal variation in incidence was found to occur with infections due to the influenza viruses, respiratory syncytial virus, and parainfluenzavirus type 1. observations made previously on the age distribution of specific respiratory viral infections, and the association of the viruses with various re ... | 1986 | 3010799 |
| presence of respiratory viruses in middle ear fluids and nasal wash specimens from children with acute otitis media. | during a 28-month period, 84 children with acute otitis media were studied by viral and bacterial cultures of middle ear fluid and viral cultures of nasal lavage fluid. viruses were isolated from the middle ear fluid of 17 (20%) patients. evidence of viral infection was demonstrated by positive viral cultures of middle ear fluid and/or nasal lavage fluid in 33 (39%) patients. rhinovirus in one patient and influenza b virus in another were the only pathogens isolated. influenza virus, enterovirus ... | 1986 | 3010225 |
| evaluation of cell line 293 for virus isolation in routine viral diagnosis. | cell line 293, a continuous line of transformed human embryonic kidney cells, has been recognized for its sensitivity in the isolation of adenoviruses, particularly the fastidious species 40 and 41, from stool specimens. to explore the possibility of using this cell line for the isolation of other viruses from clinical specimens, 293 cells were tested for their susceptibility to a variety of viruses including herpes simplex virus, parainfluenza viruses, respiratory syncytial virus, and the enter ... | 1986 | 3009540 |
| simultaneous infections with different enteric and respiratory tract viruses. | infants and young children with rotavirus (rv) or visualized adenovirus in their stools were tested for the simultaneous presence of a respiratory viral pathogen in their upper respiratory tract. overall, at least 10.7% of 484 study subjects had such dual infections, including 8.3% of 385 rv-positive gastroenteritis patients and 24.3% of 37 rv-positive respiratory disease patients. respiratory syncytial virus was present in 34.1% of 41 dual infections with rv and at least 40% of the 12 to 15 dua ... | 1986 | 3009527 |
| rapid diagnosis of respiratory virus infections in patients with acute respiratory disease. | viral respiratory infections represent a significant segment of the total respiratory disease spectrum; however, until recently the laboratory diagnosis of viral respiratory infections was relatively inefficient. development of new and improved immunologic assay systems has paved the way for accurate and reliable rapid diagnostic tests that detect viral antigens in clinical specimens. we conducted a careful and elaborate study in which radioimmunoassay for antigen detection was compared with a b ... | 1986 | 3009083 |
| effect of recombinant dna-derived bovine and human interferons on replication of bovine herpesvirus-1, parainfluenza-3, and respiratory syncytial viruses. | antiviral effects of recombinant dna-derived bovine (bo) and human (hu) interferons (ifn) on the replication of bovine herpesvirus-1, parainfluenza-3, and respiratory syncytial viruses were studied. bovine monolayer cultures were treated with recombinant dna-produced bo ifn-alpha 1, bo ifn-beta 2, hu ifn-alpha a, or hu ifn-alpha a/d and then challenge exposed with bovine herpesvirus-1, bovine parainfluenza-3 virus, bovine respiratory syncytial virus, or vesicular stomatitis virus. treatment with ... | 1986 | 3008606 |
| serosurvey for selected pathogens in hunter-killed pronghorns in western nebraska. | exposure of pronghorns (antilocapra americana) in western nebraska in 1983 to selected livestock pathogens was examined by serology and attempted virus isolation. antibodies were present to the agents of bluetongue, epizootic hemorrhagic disease, and bovine respiratory syncytial virus. there were no serologic reactors to brucella, and attempts to isolate the viruses of bluetongue and epizootic hemorrhagic disease were negative. | 1986 | 3005667 |
| role of respiratory viruses in exacerbations of primary nephrotic syndrome. | to determine whether respiratory virus infections (uri) are associated with exacerbation of nephrotic syndrome (ns) in childhood, a prospective two-winter study of 32 children with ns was done. we obtained pre- and post-season viral serologic studies, biweekly nose and throat viral cultures, daily urinalysis, biweekly telephone follow-up for uri and renal complaints, and clinical assessments as indicated. when a uri occurred, viral cultures were done weekly if the child was at home and twice wee ... | 1986 | 3005537 |
| pneumonia associated with infection with pneumocystis, respiratory syncytial virus, chlamydia, mycoplasma, and cytomegalovirus in children in papua new guinea. | paired serum samples were collected from 94 children with pneumonia admitted to goroka hospital, papua new guinea. all but three of the children were aged 1-24 months. only nine children were malnourished, with weight for age less than 70% of the harvard median (three had weight for age less than 60% of the harvard median). pneumocystis carinii antigen was detected in the serum of 23 children. twenty two children had serological evidence of recent infection with respiratory syncytial virus. five ... | 1986 | 3002538 |
| the effects of acute respiratory virus infection upon tracheal mucous transport. | tracheal mucous velocity was measured in 13 healthy non-smokers using a radioisotope-labeled aerosol and a multidetector probe during respiratory virus infections. the movement of boluses of tracheal mucous were either absent or reduced in number in five subjects with myxovirus infection (four influenza and one respiratory syncytial virus) within 48 hr of the onset of symptoms and in four subjects 1 wk later. one subject with influenza still had reduced bolus formation 12-16 wk after infection. ... | 1985 | 3002287 |
| seroepidemiologic survey for antibodies to selected viruses in the respiratory tract of lambs. | a serologic study was conducted to determine the prevalence of antibodies to, and infection rate of, mastadenovirus ovi 5, m ovi 6, parainfluenza-3 (pi-3) virus, bovine herpesvirus-1 (bhv-1), respiratory syncytial virus (rsv), bovine viral diarrhea (bvd) virus, and ovine progressive pneumonia (opp) virus in lambs at a ram lamb growth-rate test station. for 2 consecutive years, serum samples were prepared from blood collected from 1- to 2-month-old ram lambs as they entered the test station (1st ... | 1985 | 3002217 |
| parainfluenza virus bronchiolitis. epidemiology and pathogenesis. | an investigation of the epidemiology and pathogenesis of bronchiolitis due to parainfluenza virus (pv) was carried out. bronchiolitis due to pv occurred most commonly in non-caucasian males. breast-fed infants exhibited a reduced risk of developing bronchiolitis. once an episode of pv bronchiolitis occurred, both exposure to cigarette smoke and bottle feeding were associated with an increased frequency of recurrent wheezing, and subsequent infection with respiratory virus almost uniformly result ... | 1986 | 3002169 |
| a survey of respiratory syncytial virus and parainfluenza virus type 3 neutralising and immunoprecipitating antibodies in relation to paget disease. | the aetiology of paget disease of bone has not been established but certain features have suggested involvement of a parainfluenzalike virus. to seek further evidence of the possible role of paramyxoviruses in paget disease we have surveyed the presence of neutralising and immunoprecipitating antibodies to both respiratory syncytial virus and parainfluenza virus type 3 in the sera of patients attending a bone disease clinic. these two viruses were implicated by the sporadic observation of viral ... | 1985 | 3001223 |
| production of monoclonal antibodies against parainfluenza 3 virus and their use in diagnosis by immunofluorescence. | monoclonal antibodies were produced against parainfluenza virus type 3 (pi-3) and used to identify pi-3 clinical isolates in cell culture and pi-3 antigen in cells obtained from nasopharyngeal (np) washes of patients. two (2e9 and 4g5) of the three monoclonal antibodies characterized reacted by immunoblotting with a 67,000-dalton pi-3 protein, and one antibody (4e5) reacted with two viral proteins in the range of 29,000 to 31,000 daltons. the three monoclonal antibodies did not cross-react by in ... | 1985 | 3001131 |
| a simple method for increased recovery of purified paramyxovirus virions. | a simple method involving vigorous agitation of infected cell monolayers prior to collection of culture medium is described to greatly increase the recovery of purified virions of measles virus, respiratory syncytial virus and human parainfluenza virus. vigorous agitation of the flasks containing monolayers of infected cells increased the recovery of purified virions by at least 3- to 10-fold as judged by the intensity of [35s]methionine labeled viral proteins on sodium dodecyl sulphate-polyacry ... | 1985 | 3001119 |
| persistence of viruses in the nasopharynx of apparently healthy children aged 0-5 years. results of investigations performed in 1982-83. | virus isolation attempts were made with nasopharyngeal secretions from 400 apparently healthy 0-5-year-old children of a semi-closed community. repeated virus isolations were achieved in 25 cases subjected to periodic investigations in 1982-83. one-two reisolation(s), 1-5 months apart, of the initially detected viral agents could be obtained in 15 out of the 25 children. the following viruses were reisolated (in decreasing order of frequency): adenoviruses, respiratory syncytial virus, coxsackie ... | 1985 | 3000066 |
| nucleotide sequences of the 1b and 1c nonstructural protein mrnas of human respiratory syncytial virus. | the genes encoding the 1c and 1b mrnas of human respiratory syncytial (rs) virus are first in the order of viral transcription and encode nonstructural (ns) proteins of approximate molecular weights 14,000 and 11,000, respectively, estimated by gel electrophoresis. the complete nucleotide sequences of the 1c and 1b mrnas determined from several full-length cdna clones are described. the 1c and 1b mrnas contain 528 and 499 nucleotides, respectively, exclusive of poly(a), and encode proteins of 13 ... | 1985 | 2998021 |
| a viral antigen-bearing cell line derived from culture of paget's bone cells. | this study documents the characteristics of a viral antigen-bearing cell line derived from co-culture of the bone from a 71-year-old woman with paget's disease of bone and hep-2 cells. the cell line has survived in continuous culture for 3 1/2 years and 185 subcultures. the cells are epithelioid in appearance, produce alkaline and acid phosphatase, increase alkaline phosphatase activity in response to 1,25-(oh)2-d3 and contain receptors for 1,25-(oh)2-d3. immunofluorescent studies utilizing anti ... | 1985 | 2996574 |
| acute respiratory tract infections of children in hospital: a viral and mycoplasma pneumoniae profile. | respiratory specimens and blood were collected from all infants and children admitted with acute respiratory illness to a paediatric unit in christchurch from may to november (late autumn, winter and spring) 1983, to define the viral aetiological agents involved. a virus or mycoplasma pneumoniae was identified in 160 (50%) of 317 children studied by the rapid indirect immunofluorescence, virus culture and/or serological techniques. aetiological agents were detected in 71% of children with bronch ... | 1985 | 2991830 |
| surveillance of respiratory viral infections by rapid immunofluorescence diagnosis, with emphasis on the epidemiological development of respiratory syncytial virus infections. | surveillance of certain respiratory viral infections by applying immunofluorescence (if) examinations to samples of nasopharyngeal secretions has been evaluated using a simplified procedure for the preparation of cell smears. samples from 711 children living in different parts of norway were examined during the winter 1982/83 and a positive diagnosis was made for 290 children (41%). temporal epidemic peaks were observed for respiratory syncytial virus (rsv), parainfluenza virus type 3 and influe ... | 1985 | 2989359 |
| serum antibody to respiratory syncytial virus in goats in the uk. | 1985 | 2988182 | |
| respiratory syncytial virus fusion glycoprotein: nucleotide sequence of mrna, identification of cleavage activation site and amino acid sequence of n-terminus of f1 subunit. | the amino acid sequence of respiratory syncytial virus fusion protein (fo) was deduced from the sequence of a partial cdna clone of mrna and from the 5' mrna sequence obtained by primer extension and dideoxysequencing. the encoded protein of 574 amino acids is extremely hydrophobic and has a molecular weight of 63371 daltons. the site of proteolytic cleavage within this protein was accurately mapped by determining a partial amino acid sequence of the n-terminus of the larger subunit (f1) purifie ... | 1985 | 2987829 |
| detection of viral and chlamydial antigens in open-lung biopsy specimens. | the recovery of viruses and chlamydia trachomatis from cell cultures and the detection of their antigens in impression smears prepared from open-lung biopsy (olb) specimens from immunocompromised adults were compared. touch impression smears were prepared on three slides, each containing eight wells. olb tissue was homogenized (stomacher) and inoculated into mrc-5, primary monkey kidney, and mccoy cell cultures. the direct and indirect immunofluorescence (if) tests were used to detect antigens t ... | 1985 | 2983526 |
| antiviral chemotherapy and chemoprophylaxis. | antiviral compounds have been developed for use in chemoprophylaxis and chemotherapy of a variety of infections in humans, including those caused by influenza viruses, respiratory syncytial virus, and herpesviruses. the efficacy of several of these compounds has been demonstrated in rigorously controlled trials. advances in molecular virology have led to the identification of biochemically defined, virus-specific functions that serve as appropriate targets for the future development of antiviral ... | 1985 | 2983421 |
| identification of respiratory virus antigens in middle ear fluids of children with acute otitis media. | antigens of respiratory viruses were tested by immunoassay of the middle ear fluids and nasopharyngeal secretions of 137 children with acute otitis media. the following were found: (1) an epidemic of infection with respiratory syncytial virus (rsv) caused a significant increase in the occurrence of acute otitis media. (2) fifteen percent of the children had rsv antigens in middle ear fluid, and in 7% rsv was the sole pathogen found. adenovirus antigens were found in the middle ear fluid of 3% of ... | 1985 | 2982961 |
| antiviral effect of flavonoids on human viruses. | the effect of several naturally occurring dietary flavonoids including quercetin, naringin, hesperetin, and catechin on the infectivity and replication of herpes simplex virus type 1 (hsv-1), polio-virus type 1, parainfluenza virus type 3 (pf-3), and respiratory syncytial virus (rsv) was studied in vitro in cell culture monolayers employing the technique of viral plaque reduction. quercetin caused a concentration-dependent reduction in the infectivity of each virus. in addition, it reduced intra ... | 1985 | 2981979 |
| clinical signs following experimental lungworm infection and natural bovine respiratory syncytial virus infection in calves. | similar clinical signs have been reported in calves infected either by dictyocaulus viviparus or bovine respiratory syncytial virus. three experiments were carried out to establish the clinical picture and the course of the disease in animals with these infections. the clinical signs of calves infected with lungworm included coughing, nasal discharge, tachypnoea, abdominal breathing and pyrexia, and auscultation of their lungs revealed increased bronchial sounds. similar signs were also observed ... | 1988 | 2973675 |
| helper t cell recognition of respiratory syncytial virus in mice. | in this study we aimed to define the protein and viral subtype specificities of helper th cells to respiratory syncytial virus (rsv). balb/c mice were primed by infection with rsv, or with vaccinia viruses (vv) containing genes encoding several individual rsv proteins. priming for th cell memory was assayed by stimulating spleen cells in vitro with different rsv isolates and measuring rsv-specific interleukin 2 (il-2) release by t cells into supernatants using an il-2-dependent ctll cell line. s ... | 1988 | 2963089 |
| [respiratory syncytial virus infection in chronic bronchitis patients]. | two groups of patients with acute respiratory disease (ard) were examined: 57 (the 1st group) had chronic bronchitis, 265 patients (the 2nd group) had no previous history of chronic bronchitis. the investigation was performed using serological and immunofluorescent methods and was repeated in the course of 12-18 months. respiratory syncytial (rs) viral infection was diagnosed significantly more frequently in the 1st group whereas the frequency of influenza, parainfluenza and adenoviral infection ... | 1987 | 2960023 |
| effect of ipratropium bromide on respiratory mechanics in infants with acute bronchiolitis. | the effect of nebulized ipratropium bromide in 14 infants (mean age: 20 weeks, range: 4-41) with acute respiratory syncytial virus bronchiolitis was examined. a modified rapid chest compression technique was used to obtain partial expiratory flow-volume curves and maximum flow at functional residual capacity. passive respiratory mechanics were assessed by brief occlusion at end inspiration. thoracic gas volume was measured in a body plethysmograph. no significant difference was found in forced a ... | 1987 | 2959249 |
| local and systemic antibody response to bovine respiratory syncytial virus infection and reinfection in calves with and without maternal antibodies. | enzyme-linked immunosorbent assays for the detection of immunoglobulin m (igm), iga, igg1, and igg2 antibodies against bovine respiratory syncytial virus (brsv) were used to measure antibody responses of calves after experimental or natural infection with brsv. serially collected sera, lung lavage samples, nasal and eye secretions, and feces were tested for the presence of these antibodies. lung lavage fluids and nasal secretions were further examined for the presence of virus. after experimenta ... | 1987 | 2954996 |
| [severe bronchospasm disclosing a respiratory syncytial virus infection in a non-immunocompromised adult]. | 1987 | 2954123 | |
| study of prolonged virus infection in cattle stocks infected by bovine respiratory syncytial virus. | 1986 | 2953213 | |
| [respiratory syncytial virus infections and apnea in low birth-weight infants]. | respiratory syncytial virus infection was observed in 20 infants of less than 6 months of age who had a low weight at birth, were premature and/or presented with intra-uterine growth retardation. except for rhinitis, which was present in all cases, the symptoms were closely correlated to the gestational age: beyond 48 weeks they were those of a common lower respiratory tract infection, but below 48 weeks attacks of apnea predominated, associated or not with signs of bronchitis. the apnea was of ... | 1987 | 2953011 |
| respiratory syncytial virus infection of human mononuclear leukocytes in vitro and in vivo. | recurrent infections with respiratory syncytial virus (rsv) have been well documented despite serological evidence of prior exposure of the host and the absence of clear evidence of antigenic variation of the virus. therefore, human mononuclear leukocytes, as well as purified lymphocytes and monocytes-macrophages, were exposed to rsv in vitro and examined for expression of viral antigens by using indirect immunofluorescence with monoclonal antibodies to rsv. rsv infected both human monocytes-mac ... | 1985 | 2931491 |
| role of ige in the pathogenesis of bovine respiratory syncytial virus in sequential infections in vaccinated and nonvaccinated calves. | a study was undertaken to evaluate the possible role of ige in the pathogenesis of bovine respiratory syncytial virus (brsv) infection. fifteen calves were allotted at random to 3 treatment groups. one group of 6 calves was vaccinated with attenuated brsv vaccine before live-virus challenge exposure, another group of 6 was not vaccinated before challenge exposure, and the remaining 3 calves served as controls (nonvaccinated, nonchallenge exposed). calves of the 2 experimental groups were exposed ... | 1989 | 2930022 |
| the envelope proteins from purified respiratory syncytial virus protect mice from intranasal virus challenge. | a lyophilized subunit vaccine prepared from purified respiratory syncytial virus, which contained the envelope glycoproteins f and g and the nonglycosylated matrix protein vpm, was tested in sjl mice for its ability to protect the lungs of mice from intranasal viral challenge. initially, the mice were injected subcutaneously with one, two, or three doses of 5 or 25 micrograms of vaccine in 50% complete freund's adjuvant or with complete freund's adjuvant or phosphate-buffered saline only. althou ... | 1989 | 2928348 |
| effect of breast feeding on the development of anti-idiotype antibody response to f glycoprotein of respiratory syncytial virus in infant mice after post-partum maternal immunization. | groups of lactating balb/c mice were immunized in the immediate postpartum period with high doses of mab (ab-1) to the f-glycoprotein (f-gp) of respiratory syncytial virus (rsv). this antibody possessed neutralizing activity against the whole virus. the immune response to f-gp was studied in the breast feeding infant mice of such mothers at regular intervals before and after weaning. all infant mice exhibited anti-f-gp activity in serum, which was detected until 6 wk of age. splenic cells of suc ... | 1989 | 2926142 |
| production of interferon in respiratory syncytial virus bronchiolitis. | production of interferon alfa in vitro was significantly reduced during acute respiratory syncytial virus bronchiolitis but subsequently returned to normal. nasopharyngeal and endotracheal interferon alfa were detected intermittently and in low concentrations. the degree of impairment of in vitro production and poor in vivo production of interferon alfa suggest the need for a therapeutic trial of nebulised or systemic interferon in acute bronchiolitis. | 1989 | 2923479 |
| a concurrent epidemic of respiratory syncytial virus and echovirus 7 infections in an intensive care nursery. | we describe concurrent outbreaks of respiratory syncytial virus (rsv) and echovirus 7 (echo 7) infections in a neonatal intensive care unit, including infants who had dual infections. seventy-three infants were identified as having rsv from january through june, 1984. during the same surveillance period echo 7 was cultured from 20 infants, and 6 infants had concurrent rsv and echo 7 and rsv were isolated, but not concurrently. this dual outbreak of rsv and echo 7 infections persisted for months ... | 1989 | 2922234 |
| respiratory syncytial virus outbreak on american samoa. evaluation of risk factors. | acute respiratory illness is one of the leading causes of childhood mortality in developing nations. we investigated an increase in hospitalizations of children less than 2 years old for severe lower respiratory tract illness (lri) from october 1986 through december 1986 on the island of american samoa. hospitalization rates were highest in children less than 6 months of age (60 of 1000 compared with 22 of 1000 for those 6 to 11 months of age, and seven of 1000 for those 12 to 24 months of age). ... | 1989 | 2916509 |
| respiratory syncytial virus pneumonia in a human immunodeficiency virus-infected man. | 1989 | 2915431 | |
| respiratory syncytial virus-specific antibody responses in immunoglobulin a and e isotypes to the f and g proteins and to intact virus after natural infection. | we studied the antibody response to the fusion (f) and attachment (g) proteins of respiratory syncytial virus and to purified intact virus in the respiratory secretions of 29 infants and children. the goal of the study was to determine whether the immune response to either of the glycoproteins occurred predominantly in the immunoglobulin a (iga) as opposed to the ige isotype, which would indicate that one protein subunit would be a better candidate as a potential vaccine. antibody responses were ... | 1989 | 2915022 |
| failure to detect antibody to bovine respiratory syncytial virus in bovine fetal serum. | sera obtained from 147 bovine fetuses estimated to be between 120 and 270 days of gestation at an abattoir were tested for antibody to bovine respiratory syncytial virus. antibody to bovine respiratory syncytial virus was not detected in any of the sera examined. based on the results of this study and a review of the literature, it appears that transplacental infection by bovine respiratory syncytial virus does not occur, or is uncommon. | 1989 | 2914217 |
| fatal respiratory syncytial virus pneumonitis in a previously healthy child. | 1989 | 2913902 | |
| characterization of bovine respiratory syncytial virus proteins and mrnas and generation of cdna clones to the viral mrnas. | we have characterized the proteins and mrnas of bovine respiratory syncytial (brs) virus strain 391-2 and constructed cdna clones corresponding to 9 of the 10 brs virus mrnas. the proteins of brs virus-infected cells were compared with the proteins from human respiratory syncytial (hrs) virus-infected cells. nine proteins specific to brs virus-infected cells, corresponding to nine hrs virus proteins, were identified. only a brs virus polymerase protein remains to be identified. the brs virus g g ... | 1989 | 2911122 |
| respiratory syncytial virus infections in adult bone marrow transplant recipients. | 1988 | 2898070 | |
| rapid detection of respiratory syncytial virus in clinical specimens. | results of the direct immunofluorescence (if) test in 152 clinical specimens (throat swabs) were compared with those of respiratory syncytial virus (rsv) isolation. the prevalence of rsv infections in the upper and lower respiratory tract was high especially in infants until 12 months of age. the average rsv isolation rate was 18.42%, whereas the virus antigen detection was positive in 19.74% of cases. the agreement between virus isolation and direct if was 92.1%, the sensitivity of if being 82. ... | 1987 | 2891280 |
| cell-mediated cytotoxic response to respiratory syncytial virus in infants with bronchiolitis. | a cell-mediated cytotoxic response to respiratory syncytial (rsv) was demonstrated in the peripheral blood of 4 of 22 infants with acute bronchiolitis. these 4 infants were aged 3 weeks to 3 months. no such response was found in infants older than 4 months. all 4 infants with a positive response had mild infections. | 1987 | 2888988 |
| complete sequence of the major nucleocapsid protein gene of human parainfluenza type 3 virus: comparison with other negative strand viruses. | the sequence of the major nucleocapsid protein (np) mrna and its encoded protein were deduced by sequencing a cdna clone representing the complete mrna. the cdna sequence was confirmed by dideoxynucleotide sequencing of purified viral genomic rna by primer extension using synthetic oligonucleotides. the np mrna contains 1,641 nucleotides exclusive of poly(a) and encodes an np protein of 515 amino acids. alignment of the human parainfluenza type 3 virus (pf3) np protein sequence with that of send ... | 1986 | 2878059 |
| simultaneous infection with bordetella pertussis and respiratory syncytial virus in hospitalized children. | we compared three groups of hospitalized children with bordetella pertussis infection, respiratory syncytial virus (rsv) infection and dual b. pertussis/rsv infections in an effort to establish clinical and laboratory criteria by which to distinguish children with dual infections from children infected with either organism alone. the groups were compared for admission laboratory data, history of present illness, perinatal history and immunization history. children with pertussis were more likely ... | 1986 | 2876415 |
| dynamics of some genetic marker changes of respiratory-syncytial virus strains circulating among nursery-school children. | respiratory-syncytial (rs) virus strains circulating during several years appeared polymorphic in respect of two genetic markers: the regression coefficient of infectious activity (rcia39) characterizing the isolates by their reproduction in tissue cultures at supraoptimal temperature (39 degrees c) and the regression coefficient of neutralization indices (rcni) characterizing the degree of sensitivity of the strains to antibodies. high-yield rs viruses were more often isolated from children fre ... | 1986 | 2871732 |
| ribavirin and respiratory syncytial virus. | 1986 | 2868301 | |
| cytotoxic lymphocytes in the lungs of mice infected with respiratory syncytial virus. | mice infected with respiratory syncytial virus (rsv) developed cytotoxic lymphocytes in the lungs, which lysed rsv-infected, but not uninfected cells. cytotoxic activity was greatest 7 to 9 days after infection, was virus-specific, mhc-restricted and abolished by treatment of lymphocytes with anti-thy 1.2 or with anti-lyt 2.2 sera and complement. there was a close temporal relationship between the appearance of these cytotoxic lymphocytes in the lung and clearance of virus. in contrast, rsv pers ... | 1985 | 2866227 |
| monoclonal antibodies for the rapid diagnosis of influenza a and b virus infections by immunofluorescence. | mouse monoclonal antibodies, directed against antigenic sites on influenza a and b viruses and found to be type-specific in an immunoassay, were assessed for use as diagnostic reagents in an indirect immunofluorescence assay on nasopharyngeal secretions. the influenza a antibodies were directed against nucleoprotein or matrix protein antigens and the influenza b antibodies against nucleoprotein and haemagglutinin antigens. the influenza a anti-matrix monoclonal antibody was found to give a stron ... | 1985 | 2865418 |
| the disinfection of respiratory syncytial virus by isopropanol and a chlorhexidine-detergent handwash. | two of the products commonly used to disinfect the hands of nursing staff are 70% isopropanol and aqueous chlorhexidine-detergent solutions. these have been shown to rapidly destroy respiratory syncytial virus, and are therefore suitable for use where contagious spread of this virus is suspected. the virucidal component of the chlorhexidine-detergent preparation has been shown to be primarily the detergent base and not the chlorhexidine. | 1985 | 2859328 |