Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| plasmid for the direct subcloning from lambda gt11 to produce an lt-b fusion protein. | we wished to study lt-b fusion proteins for potential as vaccines. we reasoned that a gene that was expressed as a lambda gt11 fusion protein would have a better chance of being expressed fused to lt-b. to facilitate such constructions, we modified plasmid pya3081. a bamhi-ecori-bamhi* (* is to stop codon) adapter was inserted into the single bamhi site to form a new plasmid-designated pbeb. the insert was designed so that the ecori site at the downstream end of the lt-b gene was in the same rea ... | 1994 | 8074872 |
| effect of ionizing dose rate on the radioresistance of some food pathogenic bacteria. | food pathogenic bacteria including listeria monocytogenes (1a1 and atcc 19111), staphylococcus aureus (gd13 and atcc 13565), escherichia coli o157:h7 (atcc 35150), salmonella typhimurium, yersinia enterocolitica, vibrio parahaemolyticus, and campylobacter jejuni were exposed to various rates of ionizing radiation (0.78, 2.6, and 22 kgy/h) emitted by three different 60co irradiators. d10 values (d10 is the radiation dose required to eliminate 90% of a bacterial population (one logarithmic cycle r ... | 1994 | 8069779 |
| [prevalence of enteropathogens in children with liquid diarrhea]. | between january 1 and october 31, 1991 148 samples of feces corresponding to the same number of children with acute diarrhea, aged 0-2 years, admitted to hospital o'horan of the secretariat of health in the city of merida, yucatan, mexico, were studied. stools from 105 (70.9%) patients were described as watery diarrhea; from these samples at least one enteric pathogen was detected in 49 (46.6%): a single pathogen was isolated in 37 (35.2%), and mixed infections were observed in 12 (11.4%). we is ... | 1993 | 8066329 |
| biochemical characterization of campylobacter fetus lipopolysaccharides. | lipopolysaccharides (lps) of five strains of the human and animal pathogen campylobacter fetus were electrophoretically and chemically characterized. analysis with sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that all the strains produced smooth-form lps with o side chains of relatively constant chain length. upon extraction, lps partitioned into both the water and phenol phases of phenol-water extracts, which showed that two chemical species of lps were present in each c. fe ... | 1994 | 8063409 |
| differential flagellin expression in a flaa flab+ mutant of campylobacter jejuni. | campylobacter jejuni 81116 has two genes coding for flagellin, flaa and flab. fully motile wild-type c. jejuni bacteria express the flaa gene, with no flab message being detected. a nonmotile flaa flab+ mutant, r1, produced detectable levels of flagellin b which was incorporated into truncated flagella. after r1 had invaded int-407 cells, a variant with increased motility, r1-v2, was isolated. r1-v2 produced full-length flagella and an increased amount of flagellin b. transcriptional analysis sh ... | 1994 | 8063406 |
| cell association and invasion of caco-2 cells by campylobacter jejuni. | adherence and invasion studies were conducted in monolayers of caco-2 cells. three-day-old monolayers were inoculated with campylobacter jejuni 81-176 at a bacterium/cell ratio of 1,000:1. saturation studies demonstrated time- and dose-dependent saturation curves for c. jejuni cell association and invasion into caco-2 cells. electron microscopy revealed intracellular c. jejuni located within membrane-bound vacuoles. cell association and invasion were inhibited by 0.3 and 0.5 m concentrations of ... | 1994 | 8063393 |
| cloning and expression of the hup encoding a histone-like protein of campylobacter jejuni. | a campylobacter jejuni gene, designated hup, that appears to encode a homolog of the histone-like dna-binding protein, hu, has been cloned, sequenced and expressed in escherichia coli. immunoblotting and in vitro transcription/translation analyses revealed a 11-kda protein that was produced by recombinant plasmids containing hup. the gene contains an open reading frame (orf) sufficient to encode a protein of 98 amino acids (aa) with a calculated molecular mass of 10,267 da and a predicted isoele ... | 1994 | 8063109 |
| genetic organization of the region upstream from the campylobacter jejuni flagellar gene flha. | campylobacter jejuni (cj) is a gram-bacterium that causes a diarrheal disease in humans. a cj homolog of the lcrd/flha family of proteins was recently described [miller et al., infect. immun. 61 (1993) 2930-2936]. this family includes proteins that are involved in flagellar biogenesis, such as the cj flha protein, but also includes proteins found in invasive pathogens, such as the yersinia pestis lcrd protein, that play a role in the regulation and/or secretion of virulence-related proteins. hyb ... | 1994 | 8063102 |
| aids and the gut. | there are increasing challenges for the practising gastroenterologist in treating aids-related gastrointestinal diseases. the differential diagnoses of dysphagia and odynophagia include cytomegalovirus (cmv) and herpes simplex virus (hsv) infection, non-specific aphthous ulceration and non-aids oesophageal diseases, especially reflux oesophagitis. chronic subacute abdominal pain with nausea, vomiting, early satiety and weight loss is suggestive of an obstructive lesion caused by lymphoma or kapo ... | 1994 | 8054532 |
| phylogenetic and molecular characterization of a 23s rrna gene positions the genus campylobacter in the epsilon subdivision of the proteobacteria and shows that the presence of transcribed spacers is common in campylobacter spp. | the nucleotide sequence of a 23s rrna gene of campylobacter coli vc167 was determined. the primary sequence of the c. coli 23s rrna was deduced, and a secondary-structure model was constructed. comparison with escherichia coli 23s rrna showed a major difference in the c. coli rrna at approximately position 1170 (e. coli numbering) in the form of an extra sequence block approximately 147 bp long. pcr analysis of 31 other strains of c. coli and c. jejuni showed that 69% carried a transcribed space ... | 1994 | 8045890 |
| spontaneous splenic rupture associated with campylobacter jejuni infection. | 1994 | 8044559 | |
| inflammatory and phagocytic response to experimental campylobacter jejuni infection in mice. | after intraperitoneal inoculation with campylobacter jejuni balb/c, swiss and dba mice show a peritoneal inflammatory response of different intensity. only balb/c mice have a strong peritoneal response. simultaneous intraperitoneal inoculation of c. jejuni plus fecl3 increase both inflammatory response and phagocytic activity in swiss mice, without production of diarrhea. some thermostable compounds of c. jejuni have a very strong chemotactic activity against peritoneal cells of mice, whereas a ... | 1994 | 8041305 |
| in-vitro evaluation of cefpodoxime. | in-vitro antimicrobial activity of cefpodoxime was evaluated against several microbial species by both conventional tests and additional parameters which take into consideration some of the conditions likely to be encountered in infected tissues. mics for 414 recent clinical isolates, including staphylococci, streptococci, haemophilus influenzae, moraxella catarrhalis, several enterobacteriaceae, aeromonas hydrophila and campylobacter jejuni were determined. mic values overall were similar to th ... | 1994 | 8040114 |
| selection of a gyra mutant of mycobacterium tuberculosis resistant to fluoroquinolones during treatment with ofloxacin. | a strain of mycobacterium tuberculosis resistant to ofloxacin was selected in a patient with a long history of multidrug-resistant tuberculosis eventually treated by ofloxacin combined with other second-line drugs. a mutation in the gyra gene was hypothesized to be the mechanism of acquired resistance to ofloxacin in this strain. chromosomal dna of strains mtb1, isolated before treatment and susceptible to ofloxacin (mic, 1 microgram/ml), and mtb2, isolated during treatment and resistant to oflo ... | 1994 | 8035042 |
| antimicrobial antibodies in danish slaughterhouse workers and greenhouse workers. | as a consequence of contact with animals and animal products slaughterhouse workers might be at risk of infection with pathogenic microorganisms. this hypothesis has been supported by some earlier studies. in this study 217 slaughtermen and a control group of 113 greenhouse workers were investigated for the prevalence of serum antibodies to toxoplasma gondii, campylobacter jejuni (iga and igg), yersinia enterocolitica types 3 and 9, yersinia pseudotuberculosis types i, ii, iii, iv, and v, salmon ... | 1994 | 8034365 |
| antigen profile of helicobacter pylori strains isolated from peptic ulcer patients in dhaka, bangladesh. | the whole-cell-extract and the acid-extract preparations of helicobacter pylori strains isolated from peptic ulcer patients and the nctc 11638 control strain were studied to compare their antigen profiles. besides, the anti-h. pylori-antibody profile in serum from h. pylori-infected peptic ulcer patients were also studied to define the antigen stimulating specific anti-h. pylori-antibody response in the infected patients. the whole-cell extract (wce) and the acid-extract (ae) preparations showed ... | 1993 | 8031286 |
| inactivation of escherichia coli o157:h7, salmonellae, and campylobacter jejuni in raw ground beef by gamma irradiation. | raw ground beef patties inoculated with stationary-phase cells of escherichia coli o157:h7, salmonellae, or campylobacter jejuni were subjected to gamma irradiation (60co) treatment, with doses ranging from 0 to 2.52 kgy. the influence of two levels of fat (8 to 14% [low fat] and 27 to 28% [high fat]) and temperature (frozen [-17 to -15 degrees c] and refrigerated [3 to 5 degrees c]) on the inactivation of each pathogen by irradiation was investigated. in ascending order of irradiation resistanc ... | 1994 | 8031098 |
| [serological study of campylobacter jejuni infections in patients with guillain-barré syndrome]. | the association between campylobacter jejuni infection and guillain-barré syndrome (gbs) was investigated serologically in 52 gbs patients. the specific antibody titers of immunoglobulin g class to c. jejuni were measured in the patient' sera using an enzyme-linked immunosorbent assay with acid-extracted antigen. the analysis showed that 31 (59.6%) of the 52 patients had evidences of recent c. jejuni infections in contrast to 16 (8.9%) of 180 healthy persons. the seropositive patients were analy ... | 1994 | 8027593 |
| cloning, nucleotide sequence and characterization of a gene encoding superoxide dismutase from campylobacter jejuni and campylobacter coli. | genes encoding superoxide dismutase (sod: ec 1.15.1.1) were cloned from campylobacter jejuni nctc 11351 and campylobacter coli ua585 by heterologous complementation of a sod-deficient escherichia coli mutant. deletion analysis of the cloned c. jejuni dna assigned the sod gene to a 1.2 kb insert and this contained an open reading frame of 660 bp. the deduced gene product of 220 amino acids was 71% identical to the e. coli iron-containing sod and 60% identical to the e. coli manganese-containing s ... | 1994 | 8025686 |
| effects of helicobacter pylori on histamine and carbachol stimulated acid secretion by human parietal cells. | helicobacter pylori (h pylori) infection is associated with hypo, normal, and hypersecretory disorders of the gastric mucosa. pathophysiological pathways by which h pylori interacts with acid secretion are still unclear. the effects of h pylori on (14c) aminopyrine uptake by human parietal cells were examined as an indirect assay for acid secretion. isolated oxyntic glands were stimulated with submaximal concentrations of histamine or carbachol and incubated with sonicates of different h pylori ... | 1994 | 8020799 |
| inhibition of campylobacter jejuni colonization in chicks by defined competitive exclusion bacteria. | campylobacter enteritis in humans has been linked to consumption of chicken. reducing campylobacter jejuni colonization in chickens can potentially reduce campylobacter infections in humans. in this study, the reduction of c. jejuni colonization in chicks by oral administration of defined competitive exclusion (ce) cultures, 2.5% dietary carbohydrates, or ce cultures and carbohydrates was examined. prevention, elimination, or direct challenge of campylobacter infection was simulated by administe ... | 1994 | 8017915 |
| [bacteremia caused by quinolone-resistant campylobacter jejuni in a patient with hiv infection]. | 1994 | 8011729 | |
| genetic, enzymatic, and pathogenic studies of the iron superoxide dismutase of campylobacter jejuni. | campylobacter jejuni is a microaerobic bacterium that produces an acute, self-limiting, watery or bloody diarrhea in humans. little is known about how c. jejuni causes disease or even what specific capabilities it requires for survival in vivo. the enzyme, superoxide dismutase (sod), which catalyzes the breakdown of superoxide radicals to hydrogen peroxide and dioxygen is one of the bacterial cell's major defense mechanisms against oxidative damage. a pcr-based search for sod genes in c. jejuni ... | 1994 | 8005660 |
| immunological properties and ganglioside recognitions by campylobacter jejuni-enterotoxin and cholera toxin. | the immunological properties of campylobacter jejuni enterotoxin (cjt) and cholera toxin (ct) were compared by enzyme-linked immunosorbent assay (elisa) and western blotting analysis with antiserum against each toxin. antibody against cjt recognized the 68, 54 and 43 kda polypeptides of cjt and the 11 kda subunit of ct, whereas antibody against ct recognized the 68 and 54 kda polypeptides of cjt and 11 kda subunit of ct. the immunological reactions between the heterogeneous combinations of toxin ... | 1994 | 8004056 |
| the immediate bactericidal effect of lactic acid on meat-borne pathogens. | the kinetics of the bactericidal effect of lactic acid decontamination (lad) on meat-borne pathogens (salmonella spp., campylobacter jejuni and listeria monocytogenes) was studied in an in-vitro model. the bactericidal effect was greatest on organisms in the lactic acid film that replaced the natural fluid on the meat surface during lad. a stepwise increase in ph from 2.6 to 3.5 and 4.0 progressively reduced the bactericidal effect of decontamination. for treatment with 2% lactic acid for 30-90 ... | 1994 | 8002475 |
| a survey of campylobacter jejuni in broilers from assignment to slaughter using dna-dna hybridization. | a survey of campylobacter jejuni in the cecal contents of broilers raised on a farm was carried out by the dna-dna hybridization method from the day of assignment to slaughter at about 1-week intervals. c. jejuni was detected in chickens as early as 1 week of age, and was widely detected at each week of age throughout the growing period. in addition, of 20 chickens tested just after assignment, 7 (35%) were c. jejuni positive. it is suggested that newly introduced chickens may have already been ... | 1994 | 7999894 |
| [guillain-barré syndrome]. | sera from patients with guillain-barré syndrome (gbs) following campylobacter jejuni infection have autoantibody to gm1 ganglioside in the acute phase of the illness. there is a strong association between gbs and penner's serotype 19 (pen 19) of c. jejuni. the terminal structure of the bacterial lipopolysaccharide is identical to the terminal tetrasaccharide of gm1 ganglioside. anti-gm1 antibody inhibits motoneuron excitability. molecular mimicry between infectious agents and nerve tissue compon ... | 1994 | 7996693 |
| production and viability of coccoid forms of campylobacter jejuni. | studies were conducted into the formation and physiological state of coccoid cells of a strain of the human and animal pathogen campylobacter jejuni. it was found that growth phase and the presence of chloramphenicol did not affect the rate of shape transformation from spiral to coccoid, while nutrient limitation, aeration of the medium and the presence of free-radical scavengers had profound effects. coccoid cells were found to reduce the tetrazolium salts int (2-(p-iodophenyl)-3-(p-nitrophenyl ... | 1994 | 7989256 |
| effect of trisodium phosphate on biofilm and planktonic cells of campylobacter jejuni, escherichia coli o157: h7, listeria monocytogenes and salmonella typhimurium. | trisodium phosphate (tsp) has been approved by the united states department of agriculture as a post-chill antimicrobial treatment for raw poultry. this study examines the effectiveness of tsp against planktonic (suspended) and biofilm (attached) cells of campylobacter jejuni, escherichia coli o157:h7, listeria monocytogenes and salmonella typhimurium at room temperature (rt) and 10 degrees c. at either temperature e. coli o157:h7 was the most sensitive to tsp treatments; 10(6) cfu/ml of plankto ... | 1994 | 7986678 |
| metabolic activity of pathogenic bacteria during semicontinuous anaerobic digestion. | in natural environments such as anaerobic digesters, bacteria are frequently subjected to the stress of nutrient fluxes because of the continual changes in the flow of nutrients, and to survive, they must be capable of adapting readily to nutrient changes. in this study, the metabolic activities of escherichia coli, salmonella typhimurium, yersinia enterocolitica, listeria monocytogenes, and campylobacter jejuni were studied within culture bags (versapor-200 filters, 0.22-microns pore size) in l ... | 1994 | 7986040 |
| detection of flagellar antigen of campylobacter jejuni and campylobacter coli in canine faeces with an enzyme-linked immunosorbent assay (elisa)--new prospects for diagnosis. | a new diagnostic procedure was developed to detect the flagellar antigen of campylobacter jejuni and campylobacter coli in canine faecal specimens and was tested on faecal samples from random-source dogs obtained from the local dog pound. extraction of acid-soluble proteins was performed on faecal specimens and the extracted material was evaluated using species-specific monoclonal antibodies in an enzyme-linked immunosorbent assay. the assay detected all c. jejuni or c. coli infected specimens c ... | 1994 | 7975201 |
| [effect of preservatives on survival of campylobacter jejuni in ground pork meat]. | campylobacter jejuni strains: pen 2, 3, 6, 10 and 20 isolated from the food-borne infections in humans were tested. fresh ground pork samples supplemented with chemical preservatives: sodium chloride--24,000 mg/kg, sodium nitrite--125 mg/kg, potassium nitrate--500 mg/kg, sodium ascorbate--300 mg/kg and polyphosphate (hamine s)--3000 mg/kg were contaminated by c. jejuni strains. survival of c. jejuni in ground pork was determined immediate after the contamination and over a 2-d period at 4 degree ... | 1993 | 7973410 |
| [unusual forms of bacteremia due to campylobacter jejuni in patients with hiv infection]. | 1994 | 7967876 | |
| severe campylobacter jejuni diarrhoea in a patient with hodgkin's disease. | 1994 | 7963346 | |
| [a patient with guillain-barré syndrome in association with campylobacter jejuni enteritis (pen19:lio7) in a patient with hla-b52 antigen]. | we report a 15-year-old boy with guillain-barré syndrome (gbs) in association with campylobacter jejuni enteritis. a neurologic examination revealed distal-dominant weakness and areflexia. compound muscle action potentials were markedly reduced in amplitude, but the decrease in motor conduction velocities was slight. c. jejuni was isolated from his stool culture and the serotype belonged to pen19:lio7. thin-layer chromatography-immunostaining showed that his serum igg reacted strongly with gm1 a ... | 1994 | 7955736 |
| [the endotoxins of gram-negative bacteria: their structure and biological role]. | main attention in the paper is paid to the study of lipid a, a component possessing endotoxic activity. lipids a containing glucosamine disaccharide (representatives of enterobacteriaceae family), and variants of lipid a differing from the toxic one either in the structure of carbohydrate core or in the spectrum of fatty acids are considered. they are either phototrophic, nodulating (bradyrhyzobium species) or soil species (nitrobacter and thiobacillus) bacteria. lipid a from lipopolysaccharides ... | 1994 | 7952230 |
| [acute cholecystitis caused by campylobacter jejuni]. | 1994 | 7948125 | |
| [campylobacter gastroenteritis in patients with human immunodeficiency virus infection]. | campylobacter bacteria are frequent, and usually slight causes of diarrhea in a normal host while in an immunosuppressed host the diarrhea may lead to severe pictures. the aim of this study was to analyze the clinical features of gastroenteritis by campylobacter spp. in hospitalized seronegative patients and in those with hiv infection. | 1994 | 7948112 |
| persistent, recurring diarrhea in a colony of orangutans (pongo pygmaeus) caused by multiple strains of campylobacter spp. | a colony of 10 orangutans (pongo pygmaeus) experienced persistent, recurring diarrhea caused by multiple infections with campylobacter jejuni and c. coli. infections appeared to have occurred through several mechanisms, including fecal-oral transmission between orangutans, and possibly transmission by houseflies contaminated with the organisms from nearby chicken feces. among the 14 fecal and environmental c. jejuni isolates, 4 different antibiotic susceptibility profiles were detected; there we ... | 1994 | 7942349 |
| clinical aspects of campylobacter jejuni infections in adults. | campylobacter jejuni is an almost ubiquitous, microaerophilic, gram-negative rod. outbreaks have been associated with drinking raw milk or contaminated water and eating poultry. campylobacter jejuni accounts for 3.2% to 6.1% of cases of diarrheal illness in the general population of the united states, and infected patients frequently present with abdominal pain and fever. less frequently, c jejuni is responsible for bacteremia, septic arthritis, septic abortion, and other extraintestinal infecti ... | 1994 | 7941533 |
| [etiology, diagnosis and course of infectious diarrhea in the liestal canton hospital (5-year retrospective study)]. | between 1987 and 1991 219 patients (1.3% of all hospitalized patients) with acute infectious diarrhea were investigated retrospectively. 52% of the patients were hospitalized and 48% were outpatients. in 55% the inducing diarrhea microorganism could be identified. the most frequently detected pathogens were endemic salmonella sp. and campylobacter jejuni (65%). imported diarrheas (shigella sp. and parasites) were rare, as only 15% of the patients had a history of travel. all clostridium difficil ... | 1994 | 7939515 |
| henoch-schönlein purpura associated with campylobacter jejuni enteritis. case report. | after an episode of enteritis caused by c. jejuni a 15-year-old boy developed schönlein-henoch purpura. because of continuous proteinuria a renal biopsy was performed. light microscopy revealed focal proliferative glomerulonephritis. iga was the dominant immunoglobulin. a causal relationship between enteritis caused by c. jejuni and schönlein-henoch purpura complicated with focal proliferative glomerulonephritis is suggested. | 1994 | 7939469 |
| development of a tissue culture assay system for campylobacter jejuni cytotoxin and the influence of culture conditions on cytotoxin production. | a tissue culture assay system to detect campylobacter jejuni cytotoxin was developed, and the effect of culture conditions on cytotoxin production was examined. broth-culture filtrates from clinical isolates were used. the expression of cytotoxicity was dependent on the presence of serum in the culture medium. although the titre of cytotoxin was low, the greatest cytotoxicity was revealed when newborn calf (ncs), adult bovine or goat serum was added to minimum essential medium. moderate or weak ... | 1994 | 7932615 |
| seroreactivity to campylobacter jejuni and gangliosides in patients with guillain-barré syndrome. | seroreactivities to campylobacter jejuni or campylobacter coli and to the gangliosides gm1 and gd1b were studied by quantitative and immunoglobulin class-specific elisas in 42 patients with recent onset of acute guillain-barré syndrome (gbs), in 39 patients with positive campylobacter serology but no neurologic disease, and in 52 healthy blood donors. gbs patients showed positive reactivities to c. jejuni in 57%, 26%, and 2% for igg, iga, and igm, respectively, while blood donors had correspondi ... | 1994 | 7930724 |
| expression of a novel autoantibody defined by the vh3-15 gene in inflammatory bowel disease and campylobacter jejuni enterocolitis. | this study newly introduces anti-vh mabs to assess the role of clonal b cell activity in inflammatory bowel disease. immunohistochemistry of colonic biopsies in ulcerative colitis (uc) and crohn's disease (cd), but not unaffected individuals, demonstrated uniform staining of intravascular erythrocytes with bk2, a monoclonal specific for the vh3-15 ig heavy chain gene product. staining was caused by erythrocytes opsinized in vivo by anti-erythrocyte abs present in patient sera and by using the vh ... | 1994 | 7930592 |
| biodiversity of apidaecin-type peptide antibiotics. prospects of manipulating the antibacterial spectrum and combating acquired resistance. | insects have a unique repertoire of peptide antibiotics but, to date, prospects of clinical applications are not clear. apidaecin, a small peptide isolated from honeybees, inhibits viability of gram-negative bacteria; lethal activity is near immediate, independent of a conventional "lytic" mechanism, and involves stereoselective recognition of target molecules. here we report structural analysis of 14 naturally occurring apidaecin-type peptides and the existence of evolutionarily conserved ("con ... | 1994 | 7929322 |
| mechanisms of quinolone resistance. | two mechanisms of resistance to fluoroquinolones are known: (i) alteration of the molecular target of quinolone action-dna gyrase, and (ii) reduction of the quinolone accumulation. mutations altering the n-terminus of the gyrase a subunit, especially those around residues ser83 and asp87, significantly reduce the susceptibilities towards all quinolones, while alterations of the gyrase b subunit are rarely found and are of minor importance. reduced drug accumulation is associated with alterations ... | 1994 | 7927834 |
| heat shock- and alkaline ph-induced proteins of campylobacter jejuni: characterization and immunological properties. | the protein response to physiological stress was characterized in campylobacter jejuni 81176 after exposure to heat and ph shock and following periods of recovery. immunoreactivities of major stress-related proteins were determined with anti-campylobacter immune rabbit serum and intestinal lavage fluid. distinct proteins with molecular masses ranging from 10 to 120 kda were induced and/or released by selective heat or ph treatments. the most notable responses were those of two proteins with appa ... | 1994 | 7927682 |
| [toxic megacolon with colonic perforation complicating campylobacter jejuni ileo-colitis]. | campylobacter jejuni is an enteropathogen with invasive ability which may be responsible for acute colitis. we report the case of a 38-year-old woman who was hospitalized for severe acute colitis. stool specimen examination revealed campylobacter jejuni. a laparotomy was performed due to clinical deterioration and toxic megacolon. colic perforations were observed. the examination of the colectomy specimen revealed ulcerative pancolitis. this is the first published case of campylobacter jejuni in ... | 1994 | 7926445 |
| flagellin gene polymorphism analysis of campylobacter jejuni infecting man and other hosts and comparison with biotyping and somatic antigen serotyping. | flagellin gene sequence polymorphisms were used to discriminate amongst 77 strains of campylobacter jejuni from sporadic and outbreak-associated human enteric infections, and from chickens, sheep and calves. the results were assessed in relation to lior biotyping and serotyping (penner somatic antigens). eight dna pcr-rflp patterns (genotypes) were identified by analysis of hinfi fragment length polymorphisms in flagellin gene (flaa) polymerase chain reaction (pcr) products. one genotype (f-1) w ... | 1994 | 7925661 |
| [campylobacter jejuni and hiv infection]. | to evaluate the characteristics of the campylobacter jejuni infection in hiv positive patients. | 1994 | 7912110 |
| pcr-based restriction fragment length polymorphism analysis of dna sequence diversity of flagellin genes of campylobacter jejuni and allied species. | genotypes were analysed within 29 geographically diverse strains of campylobacter jejuni mainly from human enteritis cases and four strains representing c. coli, c. lari and c. helveticus. a 1723 bp dna fragment, amplified by the polymerase chain reaction (pcr) from the flagellin (fla) genes, was used to probe genomic southern blots for restriction site variation at homologous loci. internal hin fl restriction fragment length polymorphisms (rflp) of the fla gene pcr amplification product were al ... | 1993 | 7908409 |
| [hemolytic-uremic syndrome after verotoxin-producing escherichia coli infection]. | the haemolytic uraemic syndrome, first described in 1955 by gasser, is the number one cause of acute renal failure in infants. there are three types of the haemolytic uraemic syndrome: the seasonal epidemic form with prodromic diarrhoea and generally favourable outcome which usually occurs in infants, a less typical form without signs of digestive tract involvement and no seasonal prevalence which occurs more readily in older children and sometimes in families has a less favourable prognosis, an ... | 1995 | 7899353 |
| variation of the flagellin gene locus of campylobacter jejuni by recombination and horizontal gene transfer. | the capacity of campylobacter jejuni to generate genetic diversity was determined for its flagellar region. recombination within a genome, as well as recombination after the uptake of exogenous dna, could be demonstrated. the subunit of the flagellar filament of c. jejuni is encoded by two tandem genes, flaa and flab, which are highly similar and therefore subject to recombination. a spontaneous recombination within this locus was demonstrated in a bacterial clone containing an antibiotic-resist ... | 1995 | 7894725 |
| biotyping of campylobacter jejuni and campylobacter coli infections in spain. | biochemical profiles were studied in 102 campylobacter isolates from patients affected by enteric disease in orense (spain) over a 1-year period. the isolates were identified as hippurate-positive campylobacter jejuni (n = 90), campylobacter coli (n = 10) and hippurate-negative c. jejuni (n = 2). seventy-seven of the hippurate-positive and both hippurate-negative c. jejuni isolates were biotyped as c. jejuni subsp. jejuni biotype 1, nine as c. jejuni subsp. jejuni biotype 2 and four as c. jejuni ... | 1994 | 7884224 |
| intestinal carriage of campylobacters, salmonellas, yersinias and listerias in pigeons in the city of barcelona. | the faecal bacterial flora of pigeons, which may be the source of infectious diseases in man, was studied in the city of barcelona. four hundred cloacal specimens were examined for campylobacter jejuni, camp. coli, salmonella spp., yersinia spp. and listeria spp., over a 12 month period. campylobacter jejuni was the most frequently isolated micro-organism, found in 105 pigeons (26.2%), with a greater incidence in the districts of the city with a high density of pigeons and without seasonal varia ... | 1995 | 7883642 |
| [infection of ascitic fluid caused by campylobacter jejuni in a patient with liver cirrhosis. a new case with an atypical presentation]. | 1994 | 7875460 | |
| direct milk excretion of campylobacter jejuni in a dairy cow causing cases of human enteritis. | consumption of milk contaminated with campylobacter jejuni has been described as a cause of human enteritis. although faecal contamination of milk with the organism has frequently been described, direct milk excretion of campylobacter jejuni into milk has rarely been linked with cases of human infection. we describe the investigations undertaken following the isolation of campylobacter jejuni from samples of unpasteurized milk prior to retail. results of epidemiological investigations including ... | 1995 | 7867733 |
| urease-mediated destruction of bacteria is specific for helicobacter urease and results in total cellular disruption. | the survival of helicobacter mustelae, proteus mirobilis, escherichia coli and campylobacter jejuni in the presence of urea and citrate at ph 6.0 was examined. h. mustelae, which has urease activity similar to h. pylori, had a markedly reduced survival, median 2.5% (0-78%) (p < 0.001) when incubated under these conditions. only 7% of the ammonia produced by h. mustelae urease activity was recovered from the buffer, a similar percentage to that previously reported with h. pylori. none of the othe ... | 1994 | 7866348 |
| campylobacter hyoilei sp. nov., associated with porcine proliferative enteritis. | campylobacter hyoilei sp. nov. is the name proposed for an organism formerly described as strain rmit 32at (t = type strain) and a group of similar bacteria isolated from intestinal lesions of pigs with proliferative enteritis. the phenotypic characteristics of these organisms indicated that they are closely related to each other and are not strains of other campylobacter spp. commonly isolated from pigs. the results of probing of clai-, ecorv-, or bglii-cleaved genomic dnas from c. hyoilei stra ... | 1995 | 7857809 |
| practice guidelines for ordering stool cultures in a pediatric population. alberta children's hospital, calgary, alberta, canada. | a comprehensive utilization review was done of all stool culture tests performed at a pediatric hospital during a 3-year period from june 1, 1989 through to july 1, 1992. a total of 4,460 stool culture specimens were surveyed from 3,420 children. sixty percent (2,692) of the workload was from inpatients, 22% (1,001) was from emergency room (er) visits and 18% (767) was from outpatient clinic/office visits. a total of 9% (294 of 3,420) of the children were confirmed to have enteric bacterial infe ... | 1995 | 7856556 |
| cryptolepis sanguinolenta activity against diarrhoeal bacteria. | cryptolepine is the main alkaloid of cryptolepis sanguinolenta (lindl.) schlechter, a plant used in traditional medicine in west africa. the minimal inhibitory concentrations (mics) of cryptolepine, ethanol and aqueous extracts of cryptolepis sanguinolenta root were determined for 65 strains of campylobacter jejuni, 41 strains of campylobacter coli isolated from sporadic cases of gastroenteritis in portugal and 86 strains of vibrio cholerae isolated from patients with enteric infections in angol ... | 1994 | 7853867 |
| impairment of na+,k(+)-atpase activity following enterotoxigenic campylobacter jejuni infection: changes in na+, cl- and 3-o-methyl-d-glucose transport in vitro, in rat ileum. | unidirectional fluxes of na+, cl- and 3-o-methyl-d-glucose (3-mg) were measured in vitro across campylobacter jejuni live culture-infected and control rat ileal short-circuited tissues by the ussing chamber technique. net secretion of na+ and enhanced secretion of cl- ions was observed in the infected animals (p < 0.001, n = 6) as compared to the net absorption of na+ and marginal secretion of cl- ions in the control animals. there was a significant decrease in the mucosal-to-serosal fluxes of 3 ... | 1994 | 7851745 |
| [an enzyme-linked immunosorbent assay for the detection of igg antibodies against urease of helicobacter pylori]. | we have developed an enzyme-linked immunosorbent assay (elisa) for the detection of igg antibodies against helicobacter pylori (hp) using purified hp urease as an antigen. the urease was purified from ultrasonicated extract of hp by nacl linear gradient system on deae-sepharose 4b chromatography. two molecular weight bands, 65kd and 27kd were observed on a sds-page gel in the purified urease sample. the urease antigen did not crossreact to rabbit antibodies prepared against campylobacter coli an ... | 1994 | 7844892 |
| the pathogenesis of campylobacter jejuni. | studies on the pathogenesis of campylobacter jejuni show that for this organism to cause disease the susceptibility of the host and the relative virulence of the infecting strain are both important. infection with c. jejuni results from the ingestion of contaminated food or water, and the infective dose can be as low as 800 organisms. to initiate infection the organism must penetrate the gastrointestinal mucus, which it does by using its high motility and spiral shape. the bacteria must then adh ... | 1994 | 7841837 |
| antibodies against some bacterial antigens in children. | the prevalence of bacterial antibodies was determined in 173 children aged 0-15 years. the prevalence of igg borrelia burgdorferi antibodies in titres > 500 in children less than 8 years of age was 6% while none of the older children had these antibodies in titres > 400. igg helicobacter pylori antibodies were detected only in children older than 6 years of age, with a prevalence of 6.5%, as were iga h. pylori antibodies, with a prevalence of 3.7%. the prevalence of high-titre igg campylobacter ... | 1994 | 7841725 |
| agammaglobulinaemia. | agammaglobulinaemia is the most common of the primary immunodeficiencies. three major types can be distinguished: x-linked agammaglobulinaemia, early-onset agammaglobulinaemia and late-onset agammaglobulinaemia. in x-linked agammaglobulinaemia, the molecular defect has been elucidated, and genetic counseling, prenatal diagnosis and carrier detection have become important issues. the pathogenesis of early- and late-onset agammaglobulinaemia is heterogeneous and usually not within the b-cell linea ... | 1994 | 7838240 |
| enteropathogens associated with acute diarrhoeal diseases. | five types of escherichia coli are responsible for as much as 25% of all diarrheal diseases in developing countries. they tend to be transmitted via contaminated foods, particularly weaning foods, and water. they include enterotoxigenic, enteropathogenic, enteroadherent, enteroinvasive, and enterohemorrhagic e. coli. shigella species are responsible for 10-15% of acute diarrheas in children less than 5 years old and the most common etiologic agents of childhood dysentery. shigellosis is common i ... | 1994 | 7835992 |
| [in vitro antibacterial activity of fleroxacin (flrx) against clinical isolates from bacterial enteritis]. | antibacterial activity of fleroxacin (flrx), a new quinolone antimicrobial, against 36 strains of shigella app., 14 strains of salmonella spp., 11 strains of escherichia coli, 9 strains of vibrio spp. (including 2 strains of v. cholerae o1), 14 strains of campylobacter jejuni/coli, 3 strains of aeromonas spp. and 1 strain of plesiomonas shigelloides isolated from infectious enteritis patients in this study was determined. its activity was compared with that of ciprofloxacin (cpfx), norfloxacin ( ... | 1994 | 7829909 |
| the changing patterns of campylobacter jejuni/coli in lagos, nigeria after ten years. | one hundred and forty-five stool samples from children with diarrhoea at various health centres in lagos were investigated for campylobacter jejuni/coli. these organisms were isolated from 24 samples (16.5%). they were later biotyped as 23 c. jejuni and one c. coli. previous studies (3,6) recorded isolation rates of 5.2% and 11% respectively. no campylobacter species was isolated from 100 control patients who did not have diarrhoea. diarrhoea due to campylobacter jejuni/coli is still common in m ... | 1994 | 7828496 |
| immunopathogenesis and treatment of the guillain-barré syndrome--part ii. | in the second part of our review the role of antecedent infections in the pathogenesis of gbs is discussed. the association with campylobacter jejuni (c. jejuni) is highlighted and the concept of molecular mimicry, i.e., sharing of epitopes between microbes and peripheral nerve, explained. alternative mechanisms to relate an infection with the immune-mediated neuropathy are elaborated. current therapies of the gbs include plasma exchange, high-dose intravenous immunoglobulins, and supportive tre ... | 1995 | 7823973 |
| immunopathogenesis and treatment of the guillain-barré syndrome--part i. | the etiology of the guillain-barré syndrome (gbs) still remains elusive. recent years have witnessed important advances in the delineation of the mechanisms that may operate to produce nerve damage. evidence gathered from cell biology, immunology, and immunopathology studies in patients with gbs and animals with experimental autoimmune neuritis (ean) indicate that gbs results from aberrant immune responses against components of peripheral nerve. autoreactive t lymphocytes specific for the myelin ... | 1995 | 7823972 |
| identification of campylobacter jejuni, campylobacter coli and campylobacter lari by the nucleic acid amplification system nasbar. | nasbar, an isothermal amplification technique for nucleic acids, was evaluated for the specific identification of campylobacter jejuni, camp. coli and camp. lari. a set of primers and a probe were chosen from the 16s rrna sequence of campylobacter. the probe was hybridized in solution with the amplified nucleic acids of 12 campylobacter species and nine other gram-negative bacteria. the probe was shown to hybridize specifically to the amplified single-stranded rna of camp. jejuni, camp. coli and ... | 1994 | 7822229 |
| oxygen tolerance estimates in campylobacter species depend on the testing medium. | oxygen tolerance of the microaerophile campylobacter jejuni subsp. jejuni varied with different brands of complex media which were used for plating the dilute cell suspensions. the tryptone component was one factor. with some tryptones growth occurred at 21% oxygen whereas with others there was no growth at oxygen levels of 15% or higher. a chemically-defined, agar-solidified plating medium was used to estimate the oxygen tolerance of camp. jejuni subsp. jejuni, camp. coli and camp. fetus subsp. ... | 1994 | 7822225 |
| fisher syndrome after campylobacter jejuni enteritis: human leukocyte antigen and the bacterial serotype. | we describe two children who had fisher syndrome subsequent to campylobacter jejuni enteritis. the c. jejuni isolates from both patients, who lived in different areas, belonged to pen 2: lio 4. one patient had the following human leukocyte antigens (hlas): hla-a24, 33; b44, 52; dq1; and dr2, 6 antigens. another had the hla-a24, 33; b44, 54; cw1; dq1, 4; and dr4, 6. an effort should be made to isolate c. jejuni from patients with fisher syndrome and to perform hla typing so that the pathogenesis ... | 1995 | 7815224 |
| familial guillain-barré syndrome subsequent to campylobacter jejuni enteritis. | 1995 | 7815216 | |
| discrimination by means of pulsed-field gel electrophoresis between strains of campylobacter jejuni lior type 4 derived from sporadic cases and from outbreaks of infection. | the cleavage patterns of the genomic dna in 42 strains of campylobacter jejuni lior type 4, obtained from sporadic cases and from outbreaks of infection were analysed by means of pulsed-field gel electrophoresis (pfge). the cleavage of dna with smai and sali restriction enzymes showed 16 distinct fragment patterns in 20 sporadic isolates, indicating that they were heterogeneous. on the other hand, the patterns of 22 isolates derived from two outbreaks showed the same unique restriction patterns, ... | 1994 | 7806881 |
| expression of interleukin 8 and cd54 by human gastric epithelium after helicobacter pylori infection in vitro. | helicobacter pylori is associated with neutrophil infiltrates, although the mechanism of their recruitment is only partially defined. the aim of the study was to determine if kato iii, a human gastric epithelial cell line, expressed cytokines and the intercellular adhesion molecule 1 (icam-1), which could contribute to the initiation of inflammation during infection with h. pylori. | 1995 | 7806065 |
| development of resistance to macrolide antibiotics in an aids patient treated with clarithromycin for campylobacter jejuni diarrhea. | in an aids patient with diarrhea, identical isolates of campylobacter jejuni susceptible and, later, resistant to macrolide antibiotics were isolated from feces before and after treatment with clarithromycin. results of rrna gene restriction analysis and serotyping suggest that development of resistance rather than simultaneous infection with a susceptible and a resistant strain was responsible for this phenomenon. this is the first report of in vivo development of resistance by campylobacter je ... | 1994 | 7805694 |
| guillain-barré syndrome. | guillain-barré syndrome has now become recognized as a clinical syndrome that may be due to several pathological entities, consisting of an acute inflammatory demyelinating polyradiculoneuropathy as well as an acute motor axonal neuropathy. campylobacter jejuni infection is a common preceding event and, together with anti-ganglioside gm1 antibodies, is associated with axonal damage and a poor outcome. the mechanism by which such antibodies damage axons is not clear. the miller fisher syndrome is ... | 1994 | 7804457 |
| experimental colonization of mice with campylobacter jejuni. | the ability of one human and two chicken strains of campylobacter jejuni to colonise and survive in three different strains of laboratory mice (nmri, cba and c57-black) was studied. mice were inoculated orally with campylobacter jejuni and faeces samples were cultured at regular intervals during the following months. the length of colonisation of mice differed between mouse strains but also between campylobacter strains. the mouse strain c57-black was not colonised with c. jejuni to the same deg ... | 1994 | 7801521 |
| reproduction of proliferative enteritis in hamsters with a pure culture of porcine ileal symbiont intracellularis. | hamsters, three weeks old, were inoculated orally with suspensions of intracellular bacteria, grown in tissue culture cells, iec-18, rat enterocytes. cells had been infected with suspensions of intracellular bacteria derived from the lesions of proliferative haemorrhagic enteropathy occurring naturally in two pigs 916/91 and 1482/89. infected cell lines containing each separate strain, 916/91 and 1482/89, were passaged one, two or five times and pure cultures of intracellular bacteria, identifie ... | 1994 | 7801512 |
| identification of campylobacter jejuni on the basis of a species-specific gene that encodes a membrane protein. | to facilitate discrimination between the closely related enteropathogens campylobacter jejuni and c. coli, unique differences in antigenic surface structure were examined. a genomic library of c. jejuni 81116 was constructed in plasmid pbluescriptiisk- and expressed in escherichia coli k-12. rabbit hyperimmune serum raised against c. jejuni atcc 29428 recognized a clone expressing a c. jejuni 24-kda membrane-associated protein. antiserum raised against sonicated recombinant e. coli expressing th ... | 1995 | 7790451 |
| gm1 antibody in guillain-barre syndrome after campylobacter jejuni infection. | fecal culture of campylobacter jejuni was prepared by the method of skirrow, and serum class specific antibodies (igg, igm and iga) to campylobacter jejuni and serum class specific antibodies (igg igm) to gm1 were prepared with solid phase enzyme linked immunasorbent assay in 16 cases of guillain-barre syndrome (gbs), 32 controls with other neurological diseases (disease controls) and 90 normal controls. the results showed that the incidence of campylobacter jejuni infection, especially recent i ... | 1995 | 7789212 |
| campylobacter jejuni arthritis in secondary amyloidosis. | we describe a case of infectious arthritis caused by c. jejuni which developed after c. jejuni enteritis in a patient with rheumatoid arthritis and hypogammaglobulinaemia. extraintestinal infections of this enteropathogen are rare, but in patients with hypogammaglobulinaemia c. jejuni bacteriaemia is relatively common. the present patient suffered from hypogammaglobulinaemia due to a nephrotic syndrome in secondary amyloidosis. c. jejuni infection must be considered in patients with hypogammaglo ... | 1995 | 7789064 |
| prevalence of campylobacter enteritis in children from yaounde (cameroon). | a cross sectional study on the prevalence of campylobacter jejuni/coli and its possible aetiologic role in childhood diarrhoea in yaounde was undertaken in 272 children presenting with diarrhoea and 157 age matched controls from april 1989 to october 1990. stool cultures were performed according to standard techniques for campylobacter jejuni/coli shigella and salmonella species. rotavirus was detected using a latex agglutination test. campylobacter jejuni/coli was isolated from 21 (7,7 pc) of 2 ... | 1995 | 7788677 |
| discrepancy between penner serotyping and polymerase chain reaction fingerprinting of campylobacter isolated from poultry and other animal sources. | thirty-four campylobacter jejuni or coli strains, isolated from various livestock and darkling beetles from two dutch poultry farms during different broiler production cycles, were subjected to penner serotyping and polymerase chain reaction (pcr) fingerprint analysis. ten different penner serotypes were determined in the isolates. visual scoring of the pcr fingerprints resulted in 14 clearly different profiles. some strains with identical penner serotypes exhibited different pcr fingerprints an ... | 1995 | 7786504 |
| sensitivity of campylobacter spp. to irradiation in poultry meat. | the sensitivity of campylobacter jejuni (three strains), camp. coli (three strains), camp. fetus (one strain) and camp. lari (one strain) to irradiation in poultry meat was investigated. there was no significant difference in the counts obtained on blood or skirrows agar. preston agar gave a significantly lower recovery of the pathogens after irradiation so these results were not included in calculations of d10 values. the d10 values ranged from 0.12 to 0.25 kgy and there was a significant diffe ... | 1995 | 7786498 |
| [peripheral neuropathies]. | some new aspects of peripheral nerve disease are reviewed. hereditary motor and sensory neuropathy (hsmn) constitutes a genetically heterogeneous group of chronic polyneuropathies with loci mapping to chromosome 17 (type ia), chromosome 1 (type ib), and the x chromosome (x-linked hsmn). although peripheral nerve abnormalities occur in approximately 50% of diabetic patients, the frequency of severe diabetic neuropathy even in insulin-dependent persons is rather modest (6%). intensified insulin tr ... | 1995 | 7785034 |
| [a case from practice (326). dysentery (inflammatory diarrhea, cause campylobacter jejuni]. | 1995 | 7784778 | |
| the fate of potentially pathogenic bacteria in swiss hard and semihard cheeses made from raw milk. | this study examined the ability of potentially pathogenic bacteria to grow and to survive during the manufacture and ripening of swiss hard and semihard cheese varieties made from raw milk. the results show that hard cheeses are hygienically safe; 1 wk after fabrication, the inoculated pathogens (aeromonas hydrophila. campylobacter jejuni, escherichia coli, listeria monocytogenes, pseudomonas aeruginosa, salmonella spp., staphylococcus aureus, and yersinia enterocolitica) could no longer be dete ... | 1995 | 7782504 |
| restriction fragment length polymorphisms among the flagellar genes of the lior heat-labile serogroup reference strains and field strains of campylobacter jejuni and c. coli. | several typing systems have been described for campylobacter jejuni and c. coli, to assess the complex epidemiology of these important enteric pathogens. in the present study two typing methods, slide agglutination according to the lior scheme, and the demonstration of restriction-fragment length polymorphisms (rflp) of flagellar genes, have been used in parallel on a set of 194 strains. this set comprised 118 sero-reference strains of c. jejuni and c. coli of the lior scheme, as well as 76 clin ... | 1995 | 7781730 |
| [guillain-barré syndrome associated with anti-galnac-gd1a antibody subsequent to campylobacter jejuni enteritis]. | we report a 15-year-old man with guillain-barré syndrome (gbs) following campylobacter jejuni enteritis. neurologic examination revealed distal muscle weakness and mild disturbance of superficial sense on right plantar area. serial electrophysiologic studies indicated that the predominant process was axonal degeneration of motor nerves. thin-layer chromatography with immunostaining revealed that serum igg strongly reacted with galnac-gd1a and weakly with gm2, but did not react with gm1. this is ... | 1995 | 7781242 |
| incidence and transmission of antibiotic resistance in campylobacter jejuni and campylobacter coli. | one-hundred and two campylobacter clinical isolates were characterized for their in-vitro resistance to erythromycin (1.9%), three fluoroquinolones (31.3-34.3%), tetracycline (43.1%), kanamycin (4.8%), ampicillin (18.6%) and other 16 antimicrobial agents. conjugative transfer of tetracycline and kanamycin resistances among these strains was achieved and small plasmids of 4.3, 4 and 1.9 kb were observed in kanamycin-resistant campylobacter coli strains. | 1995 | 7768766 |
| isolation of the helicobacter pylori reca gene and involvement of the reca region in resistance to low ph. | to understand the potential roles of the important dna repair protein reca in helicobacter pylori pathogenesis, we cloned the reca gene from h. pylori 84-183. degenerate pcr primers based on conserved reca protein regions were used to amplify a portion of h. pylori reca, which was used as a probe to isolate the full-length reca gene from h. pylori genomic libraries. the h. pylori reca gene encoded a protein of 347 amino acids with a molecular mass of 37.6 kda. as expected, h. pylori reca was hig ... | 1995 | 7768597 |
| inhibition of dnase activity in pfge analysis of dna from campylobacter jejuni. | dnase-positive strains of campylobacter jejuni degrade their chromosomal dna during standard preparative procedures before pulsed-field gel electrophoresis (pfge). a simple method for inactivation of this dnase activity is described. formaldehyde fixation of the bacterial cells resulted in the preservation of the dna in a state suitable for restriction digestion and subsequent electrophoretic analysis. | 1994 | 7765449 |
| a pcr assay for the detection of campylobacter jejuni and campylobacter coli in water. | a pcr assay has been developed for the detection of campylobacter jejuni and camp. coli in water samples. the sample is filtered through a membrane which is subjected to sonication to release the impacted cells. after removal of the filter from the cell suspension and a freeze/thaw cell lysis step, a semi-nested pcr is carried out on the filtrate using the primers cf02, cf03 and cf04 (camp. jejuni flaa and flab gene sequences). incorporation of a sonication stage allows removal of the filter mem ... | 1994 | 7765441 |
| morphological changes of campylobacter jejuni growing in liquid culture. | campylobacter jejuni growing in liquid culture was found to exhibit gross morphological changes with time. exponentially growing cells showed typical short spiral forms. at mid-stationary phase the cells became approximately twice the length of the exponential forms. late stationary/early decline phase cells were seen to be a mixture of coccal forms and cells which were between 3 and 4 times the length of exponentially growing cells. continued incubation of cultures eventually resulted in a popu ... | 1993 | 7764089 |
| [infections caused by campylobacter jejuni in the pediatric department of the leoben district hospital 1983-1993]. | over the period 1983-1993 10,344 stool cultures were undertaken in children with gastrointestinal symptoms at the pediatric department of the landeskrankenhaus leoben. campylobacter jejuni was diagnosed as pathogen in 238 cultures taken from 196 patients aged one month to 16 years. thus, campylobacter jejuni infections (2.3% of all stool cultures) were second in frequency to various forms of salmonella (6.1% of all stool cultures; 332 patients). apart from diarrhea (92% of cases), the most frequ ... | 1995 | 7762243 |
| [miller-fisher syndrome. role of campylobacter jejuni infection]. | 1995 | 7761368 |