Publications

TitleAbstractYear
Filter
PMID(sorted descending)
Filter
first human isolate of salmonella enterica serotype enteritidis harboring blactx-m-14 in south america.we studied a clinical isolate of salmonella enterica serotype enteritidis showing resistance to oxyiminocephalosporins. pcr analysis confirmed the presence of bla(ctx-m-14) linked to is903 in a 95-kb inci1 conjugative plasmid. such a plasmid is maintained on account of the presence of a pndac addiction system. multilocus sequence typing (mlst) analysis indicated that the strain belongs to st11. this is the first report of bla(ctx-m-14) in salmonella enteritidis of human origin in south america.201222290976
characterization of a novel inactivated salmonella enterica serovar enteritidis vaccine candidate generated using a modified ci857/λ pr/gene e expression system.a new strategy to develop an effective vaccine is essential to control food-borne salmonella enterica serovar enteritidis infections. bacterial ghosts (bgs), which are nonliving, gram-negative bacterial cell envelopes, are generated by expulsion of the cytoplasmic contents from bacterial cells through controlled expression using the modified ci857/λ p(r)/gene e expression system. in the present study, the pjhl99 lysis plasmid carrying the mutated lambda pr37-ci857 repressor and phix174 lysis gen ...201222290147
modeling the inactivation of salmonella typhimurium, listeria monocytogenes, and salmonella enteritidis on poultry products exposed to pulsed uv light.pulsed uv light inactivation of salmonella typhimurium on unpackaged and vacuum-packaged chicken breast, listeria monocytogenes on unpackaged and vacuum-packaged chicken frankfurters, and salmonella enteritidis on shell eggs was explained by log-linear and weibull models using inactivation data from previous studies. this study demonstrated that the survival curves of salmonella typhimurium and l. monocytogenes were nonlinear exhibiting concavity. the weibull model was more successful than the l ...201222289588
survival of salmonella enterica, escherichia coli o157:h7, and listeria monocytogenes on inoculated walnut kernels during storage.the survival of single strains or cocktails of salmonella, escherichia coli o157:h7, and listeria monocytogenes was evaluated on walnut kernels. kernels were separately inoculated with an aqueous preparation of the pathogens at 3 to 10 log cfu/g, dried for 7 days, and then stored at 23°c for 3 weeks to more than 1 year. a rapid decrease of 1 to greater than 4 log cfu/g was observed as the inoculum dried. in some cases, the time of storage at 23°c did not influence bacterial levels, and in other ...201222289584
survival and virulence of salmonella enterica serovar enteritidis filaments induced by reduced water activity.salmonella enterica serovar enteritidis strain e40 filaments were developed under conditions of a reduced water activity (a(w)) of 0.95 in tryptic soy broth (tsb) or tryptic soy agar (tsa) supplemented with 8% or 7% nacl, respectively. filament formation was accompanied by an increase of biomass without an increase in cfu and was affected by incubation temperature and the physical milieu. the greatest amount of filaments was recovered from tsa with 7% nacl and incubation at 30°c. within 2 h of t ...201222287000
functional analysis and induction of four novel goose (anser cygnoides) avian β-defensins in response to salmonella enteritidis infection.in the current study, four novel avian β-defensins (avbds) (avbd2, 5, 9, and 10) were identified in tissues from the chinese goose (anser cygnoides). the antibacterial activity of the avbds showed that all of these avbds exhibited antibacterial activity against most of the bacteria investigated (p<0.01). in addition, antibacterial activity of all of the avbds against staphylococcus aureus and pasteurella multocida decreased significantly or was completely abolished at 150mm nacl (p<0.01). none o ...201222285691
molecular characterization of salmonella enterica serotype enteritidis isolates from humans by antimicrobial resistance, virulence genes, and pulsed-field gel electrophoresis.salmonella enterica serovar enteritidis (s. enteritidis) is a major serovar associated with human salmonellosis. a total of 425 clinical s. enteritidis isolates of human origin were collected between june 2009 and september 2010 from north carolina. the isolates were further characterized for antimicrobial susceptibility, antimicrobial resistance coding determinants, virulence genes, and fingerprint profiles to determine whether they were similar or different to the s. enteritidis strain respons ...201222283616
development and evaluation of a multiplex pcr for simultaneous detection of five foodborne pathogens.to develop a rapid multiplex pcr method for simultaneous detection of five major foodborne pathogens (staphylococcus aureus, listeria monocytogenes, escherichia coli o157:h7, salmonella enteritidis and shigella flexneri, respectively).201222268759
transcriptome sequencing of salmonella enterica serovar enteritidis under desiccation and starvation stress in peanut oil.it is well recognized that salmonella can survive long-term starvation and desiccation stresses and contaminate foods that have intermediate to low water activities; however, little is known about the specific molecular mechanisms underlying its survival and persistence in low water activity foods. in this study, we used the rna-seq approach to compare the transcriptomes (27-33 million 36-bp reads per sample) of a salmonella enterica subsp. enteric serovar enteritidis strain atcc baa-1045 after ...201222265317
impact of storage time and temperature on thermal inactivation of salmonella enteritidis pt 30 on oil-roasted almonds.whole nonpareil variety almonds were inoculated with salmonella enteritidis pt 30 and stored at 4 or 23 °c for up to 48 wk. at 1, 12, 24, 37, and 48 wk of storage, almonds were heated by immersion in 121 °c oil. after heating for 0.5 to 2.5 min, almonds were drained, transferred to tryptic soy broth, and mixed with a stomacher prior to plating onto tryptic soy and bismuth sulfite agars. over the 48 wk of storage, salmonella declined by 0.5 and 2.1 log cfu/g at 4 and 23 °c, respectively. the surv ...201222260116
growth inhibition of foodborne pathogens by oenococcus oeni.to explore the possibility of using oenococcus oeni to inhibit foodborne pathogens, and to characterize antimicrobial compounds produced by o. oeni, 24 strains of o. oeni were tested for their ability to inhibit growth of foodborne pathogens, escherichia coli o157:h7, salmonella enteritidis, and listeria monocytogenes by using the spot-on-lawn method. of the 24 strains, 17 strains were able to inhibit all 3 pathogens in this study. proteases, catalase, and buffer solutions were used for determin ...201222260113
classification and structural analysis of live and dead salmonella cells using fourier transform infrared spectroscopy and principal component analysis.fourier transform infrared spectroscopy (ft-ir) was used to detect salmonella typhimurium and salmonella enteritidis food-borne bacteria and to distinguish between live and dead cells of both serotypes. bacteria cells were prepared in 10(8) cfu/ml concentration, and 1 ml of each bacterium was loaded individually on the znse attenuated total reflection (atr) crystal surface (45° znse, 10 bounces, and 48 mm × 5 mm effective area of analysis on the crystal) and scanned for spectral data collection ...201222257216
the intestinal secreted c-type lectin reg3b attenuates salmonellosis but not listeriosis in mice.background: the reg3 protein family, including the human member designated pancreatitis-associated protein, are secreted proteins that contain a c-type lectin domain involved in carbohydrate binding. they are expressed by intestinal epithelial cells. colonization of germ-free mice and intestinal infection with pathogens increases the expression of reg3g and reg3b in the murine ileum. reg3g is directly bactericidal for gram-positive bacteria but the exact role of reg3b in bacterial infections is ...201222252863
evaluation of in vitro and in vivo adhesion and immunomodulatory effect of lactobacillus species strains isolated from chickens.this study aimed to characterize the in vitro and in vivo adhesion and immunomodulatory effect of lactobacillus strains isolated from chickens. lactobacillus samples isolated from 65-wk-old birds were identified by pcr; their adhesion was evaluated in vitro via basement membrane-type cell matrix and in vivo through carboxyfluorescein succinimidyl amino ester staining inoculation in 1-d-old birds and duodenum, jejunum, ileum, and cecum collections at 1, 4, 12, and 24 h after inoculation. the 5 be ...201222252349
differences in the early response of hatchlings of different chicken breeding lines to salmonella enterica serovar enteritidis infection.poultry products are the major source of food-borne salmonella infection in humans. broiler lines selected to be more resistant to salmonella could reduce the transfer of salmonella to humans. to investigate differences in the susceptibility of newly hatched chicks to oral infection with salmonella enterica serovar enteritidis, 3 commercial broiler lines (a, b, and c) were infected immediately after hatch and compared to healthy controls at 0.33, 1, and 2 d postinfection. weight, bacteriological ...201222252347
the role of heat shock protein 70 in resistance to salmonella enteritidis in broiler chickens subjected to neonatal feed restriction and thermal stress.environmental stressors may influence chicken performance and susceptibility to pathogens, such as salmonella enteritidis. this study was conducted to determine the effects of heat shock protein (hsp)70 expression on resistance to salmonella enteritidis infection in broiler chickens subjected to heat exposure. chicks were divided into 3 feeding regimens: ad libitum feeding (control); 60% feed restriction on d 4, 5, and 6 (fr60); and 60% feed restriction on d 4, 5, and 6 plus 1,500 mg/kg of querc ...201222252346
persistent salmonella enteritidis environmental contamination on layer farms in the context of an implemented national control program with obligatory vaccination.the aim of this study was to closely examine the salmonella enterica serovar enteritidis environmental contamination on persistently positive layer farms in belgium during successive laying cycles. all of the farms were required to vaccinate their layers under the national control program for salmonella. seven farms with previous or current salmonella enteritidis contamination were monitored during different stages of the laying period and after cleaning and disinfection (cd). environmental samp ...201222252339
characterization of the elphis prophage from salmonella enterica serovar enteritidis strain lk5.phages are a primary driving force behind the evolution of bacterial pathogens by transferring a variety of virulence genes into their hosts. similar to other bacterial genomes, the salmonella enterica serovar enteritidis lk5 genome contains several regions that are homologous to phages. although genomic analyses demonstrated the presence of prophages, it was unable to confirm which phage elements within the genome were viable. genetic markers were used to tag one of the prophages in the genome ...201222247173
analysis of immune-related gene expression in chicken peripheral blood mononuclear cells following salmonella enterica serovar enteritidis infection in vitro.we examined mrna expression of eight genes, tlr4, tlr5, tlr15, interleukin (il)-1β, il-6, transforming growth factor-β4 (tgf-β4), cxcli2, and a macrophage inflammatory protein (mip) family chemokine called ccli2, in peripheral blood mononuclear cells (pbmcs) isolated from the blood of chickens after in vitro exposure to salmonella enterica serovar enteritidis (se). the chickens of four chinese native lines, qingjiaoma, sanhuang, wugu, and xueshanma, were evaluated for mrna expression levels at 2 ...201222244688
different potency of bacterial antigens, both tlr2 and tlr4 ligands, in stimulating mature mast cells to cysteinyl leukotriene synthesis.the aim of study was to compare the potency of different bacterial antigens to induce rat mature mast cell to cysteinyl leukotriene (cyslt) generation. we examined toll-like receptor (tlr)2 agonists, i.e. lipoteichoic acid (lta) staphylococcus faecalis, streptococcus pyogenes, bacillus subtilis and staphylococcus aureus, lipoarabinomannan (lam) mycobacterium smegmatis, peptydoglican (pgn) staphylococcus aureus, as well as tlr4 agonists, i.e. lipopolysaccharide (lps) klebsiella pneumoniae, pseudo ...201222233438
effect of high hydrostatic pressure and pressure cycling on a pathogenic salmonella enterica serovar cocktail inoculated into creamy peanut butter.the ability of salmonella enterica serovars to survive in high fat content, low water activity foods like peanut butter has been demonstrated by large foodborne illness outbreaks in recent years. this study investigates the potential of high hydrostatic pressure processing, including pressure cycling, to inactivate salmonella inoculated into creamy peanut butter. a cocktail of pathogenic strains of salmonella enteritidis pt30, salmonella tennessee, salmonella oranienburg, salmonella anatum, salm ...201222221373
salmonella enteritidis rck-mediated invasion requires activation of rac1, which is dependent on the class i pi 3-kinases-akt signaling pathway.the salmonella outer membrane protein rck mediates a zipper-like entry mechanism controlled by rac, the arp2/3 complex, and actin polymerization. however, little is known about the early steps leading to rac activation and rck-mediated internalization. the use of pharmacological inhibitors or pi 3-kinase dominant-negative mutant induced more than 80% less invasion without affecting attachment. moreover, rck-mediated internalization caused an increase in the association of p85 with at least one t ...201122210834
prevalence and antimicrobial resistance of salmonella serotypes isolated from poultry in spain: comparison between 1993 and 2006.a total of 226 chicken samples (carcasses, legs, wings, necks and breasts) were obtained (73 in 1993 and 153 in 2006) from 10 retail outlets in north-western spain and screened for the presence of salmonella. isolates were subjected to serotyping, phage typing (salmonella enteritidis and salmonella typhimurium) and antimicrobial susceptibility testing (15 antimicrobials; disk diffusion method). salmonella was detected in 40 (55%) samples in 1993 and 19 (12.4%) in 2006 (p<0.001). the serotypes (s ...201122208955
A genetically engineered derivative of Salmonella Enteritidis as a novel live vaccine candidate for salmonellosis in chickens.To construct a novel live Salmonella Enteritidis (SE) vaccine candidate, SE was genetically engineered using the allelic exchange method to delete two virulence genes, lon and cpxR. The lon gene deletion is essential to impair Salmonella replication and avoid overwhelming systemic disease in the host. The cpxR gene deletion is needed to enhance the ability of bacteria to adhere and invade the host cell. Scanning electron microscopy revealed that the derivatives JOL917 (?lon), JOL918 (?cpxR), and ...201122192447
the effect of x-ray irradiation on salmonella inactivation and sensory quality of almonds and walnuts as a function of water activity.the overall goal of this study was to develop a set of process design principles for low-energy x-ray irradiation of tree nuts. almonds and walnuts were inoculated with salmonella enteritidis pt30 and salmonella tennessee, and conditioned to four different water activities (0.23, 0.45, 0.64, and 0.84 a(w)). thereafter, the inoculated/conditioned samples were irradiated to achieve up to a 5-log reduction in salmonella using a pilot scale low-energy x-ray food irradiator. greater efficacy (d(10)-v ...201122189022
effects of gamma irradiation on the viability and phenotypic characteristics of salmonella enteritidis inoculated into specific-pathogen-free eggs.the goal of this study was to determine the effects of various levels of gamma irradiation on the phenotypic characteristics of 20 strains of salmonella enteritidis inoculated separately into specific-pathogen-free shell eggs. bacterial strains were inoculated into egg yolks and exposed to (60)co radiation at doses of 0.49 to 5.0 kgy. the eggs were maintained at 25°c and analyzed for the presence of salmonella on days 1, 2, 4, and 7, and the recovered salmonella isolates were characterized bioc ...201122186042
inactivation of salmonella in biofilms and on chicken cages and preharvest poultry by levulinic acid and sodium dodecyl sulfate.surface contamination (skin and feathers) of broilers with salmonella occurs primarily during growth and transportation. immediately after transporting chickens, chicken cage doors were sprayed with a foam containing 3% levulinic acid plus 2% sodium dodecyl sulfate (sds). samples were collected for salmonella assay after 45 min. salmonella on cage doors was reduced from 19% (19 of 100 doors) before treatment to 1% (1 of 100 doors) after treatment, coliform counts were reduced from 6 to 8 to 2 t ...201122186041
Henhouse Feeding Style and Salmonella Enteritidis Contamination in Unvaccinated Flocks of Egg-Farms, April 1994-March 2001.From April 1994 to March 2001, monitoring tests (surveillance) for the contamination of Salmonella Enteritidis (SE) in commercial egg-farms and farm trace back inspections of SE food-borne illness were carried out in the Himeji Livestock Hygiene Service Center jurisdiction of Hyogo prefecture, Japan. SE vaccination was not performed before the outbreak of SE-associated food-borne illness and SE contamination of a farm was recognized. In surveillance, year average percentages of identified SE-pos ...201122185772
[comparison of usefulness of lipopolysaccharides extracted by phenol and trichloroacetic acid from salmonella typhimurium and enteritidis for serodiagnosis of salmonelosis].salmonella typhimurium and salmonella enteritidis are the two predominant serogroups, responsible for about 80% of all human cases of salmonelosis in poland. therefore we compared the usefulness of lipopolysaccharides antigens extracted by phenol (westphal method) and trichloroacetic acid (boivine method) from salmonella typhimurium and enteritidis in elisa method for the determination of antibodies. we used one home - made lps antigen and two others commercially available antigens from sigma - ...201122184899
[etiological surveillance and analysis of infectious diarrhea in beijing in year 2010].to explore the pathogenic form, epidemic features and serotype distribution of the pathogenic bacteria causing infectious diarrhea in beijing.201122177306
large outbreak of salmonella enteritidis pt8 in portsmouth, uk, associated with a restaurant.summaryseventy-five individuals with salmonella infection were identified in the portsmouth area during august and september 2009, predominantly salmonella enteritidis phage type 8. five patients were admitted to hospital. a case-case comparison study showed that a local restaurant was the most likely source of the infection with a risk of illness among its customers 25-fold higher than that of those who did not attend the restaurant. a case-control study conducted to investigate specific risk f ...201122166322
Integrated surveillance and potential sources of Salmonella Enteritidis in human cases in Canada from 2003 to 2009.SUMMARYSalmonella Enteritidis has emerged as the most prevalent cause of human salmonellosis in Canada. Recent trends of S. Enteritidis subtypes and their potential sources were described by integrating Salmonella data from several Canadian surveillance and monitoring programmes. A threefold increase in S. Enteritidis cases from 2003 to 2009 was identified to be primarily associated with phage types 13, 8 and 13a. Other common phage types (4, 1, 6a) showed winter seasonality and were more likely ...201122166269
BT cationic peptides: Small peptides that modulate innate immune responses of chicken heterophils and monocytes.Neonatal poultry exhibit a transient susceptibility to infectious diseases during the first week of life that stems from inefficient host defense mechanisms. Yet, the initial host immune response to pathogens is a critical determinant of disease resistance and susceptibility. With this context in mind, novel ways to stimulate or modulate the hosts' natural immune response is emerging as an important area of interest for food animal producers including the poultry industry. Specifically, we have ...201122129785
[construction and characterization of an ompr gene deletion mutant from salmonella enteritidis].to investigate the role of ompr gene from salmonella enteritidis in biofilm formation and virulence.201122126082
influence of various growth conditions on fresnel diffraction patterns of bacteria colonies examined in the optical system with converging spherical wave illumination.the novel optical system based on converging spherical wave illumination for analysis of bacteria colonies diffraction patterns, is proposed. the complex physical model of light transformation on bacteria colonies in this system, is presented. fresnel diffraction patterns of bacteria colonies escherichia coli, salmonella enteritidis, staphylococcus aureus grown in various conditions, were examined. it was demonstrated that the proposed system enables the characterization of morphological changes ...201122109028
A child with septic arthritis of hip: a rarely encountered cause.We report a rare case of septic arthritis in a healthy child caused by Salmonella enteritidis. No predisposing factor was detected. Salmonella enteritidis was isolated from the infected joint tissue obtained following surgical drainage. Based on the culture and sensitivity report, he was treated with a 6-week course of antibiotic. He improved dramatically without any detrimental sequelae at end of one year.201122106702
visualisation of morphological interaction of diamond and silver nanoparticles with salmonella enteritidis and listeria monocytogenes.currently, medicine intensively searches for methods to transport drugs to a target (sick) point within the body. the objective of the present investigation was to evaluate morphological characteristics of the assembles of silver or diamond nanoparticles with salmonella enteritidis (g-) or listeria monocytogenes (g+), to reveal possibilities of constructing nanoparticle-bacteria vehicles. diamond nanoparticles (nano-d) were produced by the detonation method. hydrocolloids of silver nanoparticles ...201122097468
infection of mice by salmonella enterica serovar enteritidis involves additional genes that are absent in the genome of serovar typhimurium.salmonella enterica serovar enteritidis (s. enteritidis) causes a systemic, typhoid-like infection in newly hatched poultry and mice. in the present study, a library of 54,000 transposon mutants of s. enteritidis pt4 strain p125109 was screened for mutants deficient in the in vivo colonization of balb/c mouse model using a microarray-based negative selection screening. mutants in genes known to contribute to systemic infection (e.g. spi-2, aro, rfa, rfb, phop, phoq) and enteric infection (e.g. s ...201122083712
butyrate enhances disease resistance of chickens by inducing antimicrobial host defense peptide gene expression.host defense peptides (hdps) constitute a large group of natural broad-spectrum antimicrobials and an important first line of immunity in virtually all forms of life. specific augmentation of synthesis of endogenous hdps may represent a promising antibiotic-alternative approach to disease control. in this study, we tested the hypothesis that exogenous administration of butyrate, a major type of short-chain fatty acids derived from bacterial fermentation of undigested dietary fiber, is capable of ...201122073293
suppurative thyroiditis caused by salmonella enteritidis.bacterial thyroiditis is a rare disease, and one of which the clinical symptoms and signs are frequently misleading. on the other hand, prompt diagnosis is crucial for successful treatment. we report the case of an 82 year-old man with diabetes mellitus type 2 and a history of steroid treatment who presented with severe odynophagia and dysphagia associated with fever, chills, sore throat and right ear pain. based on the clinical picture, radiological studies, thyroid cytology, blood and thyroid ...201122069108
characterization of class i integrons among salmonella enterica serovar enteritidis isolated from humans and poultry.a total of 84 salmonella enterica serovar enteritidis (s. enteritidis) isolates, 42 of human and 42 of poultry origin, were characterized for antimicrobial resistance patterns and class i integrons. among them, 58 (69%) s. enteritidis were multidrug-resistant (mdr) and showed resistance to two or more antibiotic classes. by pcr assays and dna sequencing, 50 (59.5%) s. enteritidis isolates were found to carry class i integrons. amplification of internal variable regions of class i integrons revea ...201122066813
survival and growth of escherichia coli o157:h7, yersinia enterocolitica and salmonella enteritidis on decontaminated and untreated meat.decontamination of meat or carcasses may have an effect in reducing the number of pathogens. recontamination with other pathogens during cutting or packaging may, however, result in higher growth on decontaminated than on untreated meat due to the lack of competing non-pathogenic microorganisms. in this study we compared the growth of pathogens during storage at 10°c (worst case condition) on untreated meat and meat that had been decontaminated by steam vacuuming combined with spraying with 0.2 ...200122061504
Rare case of intracranial Salmonella enteritidis abscess following glioblastoma resection: Case report and review of the literature.Worldwide, Salmonella enteritidis (SE) is becoming a common cause of gastrointestinal infections by contaminated food products, mainly eggs. Extra-intestinal manifestations such as brain abscess are more commonly associated with Salmonella typhimurium and are rare in adults. We report the clinical features, treatment outcomes and risk factors predisposing our patient to Salmonella enteritidis brain abscess and discuss relevant literature.201122059142
excision of an unstable pathogenicity island in salmonella enterica serovar enteritidis is induced during infection of phagocytic cells.the availability of the complete genome sequence of several salmonella enterica serovars has revealed the presence of unstable genetic elements in these bacteria, such as pathogenicity islands and prophages. this is the case of salmonella enterica serovar enteritidis (s. enteritidis), a bacterium that causes gastroenteritis in humans and systemic infection in mice. the whole genome sequence analysis for s. enteritidis unveiled the presence of several genetic regions that are absent in other salm ...201122039432
Detection of Salmonella Enteritidis in asymptomatic carrier animals: comparison of quantitative real-time PCR and bacteriological culture methods.Quantification of Salmonella in asymptomatic carrier animals can be used to assess microbial risk and monitor the level of contamination in domestic animals used for food production. We examined the sensitivity, specificity and accuracy of real-time qPCR, without pre-enrichment or selective enrichment stages, for the quantification of S. enterica serovar Enteritidis in resistant mice, as a model of asymptomatic carrier animal. The results were compared with those obtained by traditional bacterio ...201122033939
Breast Abscess in a Man Due to Salmonella enterica Serotype Enteritidis.Nontyphoidal salmonellae can cause breast infection only exceptionally. A case of breast abscess in a 70-year-old man due to Salmonella enterica serotype Enteritidis (Salmonella Enteritidis) is reported. The infection was successfully treated with a combination of surgical and antibiotic treatment.201222031702
inhibition of listeria monocytogenes and salmonella enteritidis on chicken wings using scallop-shell powder.the present study was designed to determine the inhibitory effect of scallop-shell powder (ssp) on listeria monocytogenes and salmonella enteritidis on chicken wings. chicken wings artificially inoculated with l. monocytogenes (8.3 log(10) cfu/g) or s. enteritidis (8.8 log(10) cfu/g) were immersed in distilled water (0.10 or 0.50% wt/vol) of ssp slurries for 10 or 30 min, respectively. the amount of l. monocytogenes, s. enteritidis, mesophilic aerobic bacteria (mab), and yeast or mold was determ ...201122010247
comparative proteomic analysis of salmonella enterica serovars enteritidis, typhimurium and gallinarum.salmonella enterica includes several related serovars which have different host ranges and cause diseases of different severities. however, their pathogenic potential is unknown, and it is not clear what mechanisms are activated or inhibited during adaptation to a specific host environment. some proteins are involved in the mechanism of pathogenicity at a molecular level and provide the functional aspects that create the diverse phenotypes. to compare proteomic analyses of the total proteins of ...201121997235
D-Lactic acidosis 25 years after bariatric surgery due to Salmonella enteritidis.D-lactic acidosis is a rare complication that occurs in patients with short bowel syndrome due to surgical intestine resection for treatment of obesity. The clinical presentation is characterized by neurologic symptoms and high anion gap metabolic acidosis. The incidence of this syndrome is unknown, probably because of misdiagnosis and sometimes symptoms may be incorrectly attributed to other causes. Therapy is based on low carbohydrate diet, sodium bicarbonate intravenous, rehydratation, antiob ...201221996046
altered pkr signalling and c/ebpβ expression is associated with hla-b27 expression in monocytic cells.infection caused by certain gram negative bacteria, e.g. salmonella, can trigger inflammatory joint disease reactive arthritis (rea). it is suggested that the disease-triggering bacteria or bacterial components persist in patients for an abnormally long time. development of rea is strongly associated with tissue antigen hla-b27. previously, we reported an enhanced replication of s. enteritidis and altered p38 map kinase signalling in hla-b27-expressing monocytic cells. here we aimed to investiga ...201121988375
[salmonella as an etiological agent of hematogenous ostheomyelitis in adults]. 201121983539
changes in the expression of toll-like receptors in the chicken testis during sexual maturation and salmonella infection.rooster infertility is a major concern in the poultry industry and chicken male reproductive organs are the infectious tissues of various pathogenic microorganisms. protection of the chicken male reproductive organs from pathogens is therefore an essential aspect of reproductive physiology. recently toll-like receptors (tlrs) have been identified as one of the key components of innate immunity in vertebrate species and have been reported to be expressed in the reproductive organs in various fema ...201121975303
Enhanced phosphorylation of STAT1 is dependent on PKR signaling in HLA-B27 expressing U937 monocytic cells.OBJECTIVE.: To study the phosphorylation of STAT1 in HLA-B27-transfected human monocytic cells and the role of signaling molecules PKR and p38 in STAT1 phosphorylation. METHODS.: U937 human monocytic cell transfectants stably expressing wild type HLA-B27 or mutated HLA-B27 heavy chains (HC) with amino acid substitutions in the B pocket were prepared. Mock transfected cells were prepared using the antibiotic resistance vectors (pSV2neo or RSV5neo) alone. PMA differentiated cells were stimulated w ...201121968657
Automated peritoneal dialysis-related peritonitis due to Salmonella enteritidis in a pediatric patient.Peritoneal dialysis (PD) represents the preferred renal replacement therapy in pediatric patients; however, the younger the age at PD initiation, the greater the risk of PD-related infections. We present here the first case report of a 14-year-old girl with automated peritoneal dialysis-related Salmonella enteritidis peritonitis. The child responded only partially to an appropriate course of antibiotic therapy;the PD catheter was therefore removed and the patient shifted to hemodialysis. Along w ...201121964761
Acanthamoeba polyphaga, a potential environmental vector for the transmission of food-borne and opportunistic pathogens.The endosymbiotic relationship could represent for many bacteria an important condition favouring their spread in the environment and in foods. For this purpose we studied the behaviour of some food-borne and opportunistic pathogens (Listeria monocytogenes, Staphylococcus aureus, Enterococcus faecalis, Salmonella enterica serovar Enteritidis, Aeromonas hydrophila, Yersinia enterocolitica) when internalized in Acanthamoeba polyphaga. Our results confirm the capability of the bacteria tested to gr ...201121953544
single nucleotide polymorphisms that differentiate two subpopulations of salmonella enteritidis within phage type.abstract:201121942987
naturally occurring motility-defective mutants of salmonella enterica serovar enteritidis isolated preferentially from nonhuman rather than human sources.salmonellosis represents a worldwide health problem because it is one of the major causes of food-borne disease. although motility is postulated as an important salmonella virulence attribute, there is little information about variation in motility in natural isolates. here we report the identification of a point mutation (t551 → g) in mota, a gene essential for flagellar rotation, in several salmonella enterica serovar enteritidis field isolates. this mutation results in bacteria that can biosy ...201121926214
rapidchek select salmonella enteritidis test system for the detection of salmonella enteritidis in poultry house drag swabs, shell egg pools, and chicken carcass rinsates.the rapidchek select salmonella enteritidis test system was validated for the detection of salmonella enteritidis (se) in poultry house drag swabs, shell egg pools, and chicken carcass rinsates. the method utilizes rapidchek select salmonella (aoac ptm license no. 080601) proprietary primary and secondary enrichment media. following enrichment, an immunochromatographic test strip is inserted into the tube containing the secondary enrichment broth, developed for 10 min, and interpreted. salmonell ...201121919348
reveal salmonella enteritidis test for detection of salmonella enteritidis in shell eggs and environmental samples.reveal salmonella enteritidis (se) is a lateral flow-based immunodiagnostic assay used for rapid detection of salmonella enterica serovar enteritidis from pooled shell eggs and environmental samples. this assay uses highly specific antibodies to accurately detect s. enteritidis. studies were conducted to compare the performance of this test against reference procedures for detection of s. enteritidis from both pooled shell eggs and environmental samples. pooled shell eggs were inoculated with lo ...201121919347
[assessment of the application of variable-number tandem repeat loci of salmonella enteritidis in subtyping multiple-locus variable-number tandem repeat analysis].to evaluate the feasibility of the application of variable-number tandem repeat (vntr) loci of salmonella enteritidis (s. enteritidis) in subtyping mutiple-locus variable-number tandem repeat analysis (mlva).201121914334
[statistical analysis of trend of human salmonella infections in poland in 1995-2007].the epidemiological situation of human salmonellosis in poland in the successive years (between 1995 and 2007), presented in this study, was illustrated by using the notification rates expressed as the number of confirmed cases (of total human salmonella infections and infections associated with chosen serovars) per 100,000 inhabitants. the notification rates were calculated basing on the published data of human salmonellosis in poland (1995-2007) and the total population in each year. their dis ...201121913488
[salmonellosis in poland in 2009].in 2009, 8 964 salmonellosis cases were reported, the incidence was 23,5 per 100 000 population. among them 8 847 cases were intestinal and 117 were extraintestinal. no deaths reported due to salmonellosis. in total 162 outbreaks were reported involving 1 580 cases. the most common type of outbreaks were household outbreaks. the most frequently isolated serotype in outbreaks and single cases was s. enteritidis. like in previous years the highest incidence was in children aged 2 (266,8 per 100 00 ...201121913468
immunogenicity of a salmonella enteritidis mutant as vaccine candidate and its protective efficacy against salmonellosis in chickens.a novel salmonella enteritidis (se) vaccine candidate strain, jol919 was constructed by deleting the lon and cpxr genes from a wild-type se using an allelic exchange method. the study was carried out to evaluate the strain as a vaccine candidate against salmonellosis. the strain showed the enhanced macrophage invasion, early bacterial clearance and higher immune responses as compared to the other mutants, jol917 (δlon) and jol918 (δcpxr), and the wild type. in further analysis, the chickens immu ...201121908057
protection activity of a novel probiotic strain of bacillus subtilis against salmonella enteritidis infection.the activity of 240 bacterial isolates screened from the gastrointestinal tracts of native chickens were evaluated for use as a potential probiotic in food animal production in order to protect against animal diseases and reduce pathogenic contamination of human food products. in observing the antagonistic activity of 117 bacilli isolates, 10 of these isolates exhibited higher growth inhibition of seven foodborne pathogens, including salmonella enteritidis, salmonella typhimurium, escherichia co ...201121907374
salmonellosis outbreak due to salmonella enteritidis phage type 14b resistant to nalidixic acid, austria, september 2010.we report on a salmonellosis-outbreak due to salmonella enteritidis phage type 14b resistant to nalidixic acid (s. enteritidis pt14b nx) among residents and employees of a student residence in austria, september 2010. the outbreak was described and analysed by a retrospective cohort study, and microbiological environmental investigations were conducted to identify the outbreak source(s) and the reservoir of the outbreak strain. a total of 66 persons fulfilled the outbreak case definition includi ...201121903036
inhibition of growth of pathogenic bacteria in raw milk by legume protein esters.protein isolates from soybean and chickpea, as well as their methylated esters, were tested for their inhibitory action against the propagation of pathogenic bacteria in raw milk during its storage either at room temperature or under refrigeration. raw milk was inoculated with a mixed culture of listeria monocytogenes scott a and salmonella enterica serovar enteritidis strain pt4 at ca. 2 log cfu ml⁻¹. aerobic plate count, coliform count, and presumptive e. coli in raw milk treated with esterifi ...201121902916
attenuated salmonella enteritidis e23 as a vehicle for the rectal delivery of dna vaccine coding for hiv-1 polyepitope ctl immunogen.this study is focusing on elucidation of the capacity of attenuated salmonella enteritidis e23 (cya, crp) to serve as a vehicle for the rectal delivery of the dna vaccine. earlier for creation hiv-1 candidate dna vaccine we have designed the polyepitope protein tci (t-cell immunogen), which comprises over 80 ctl epitopes from subtype a, b and c hiv-1 proteins. the gene coding for tci protein was used to construct the eukaryotic expression plasmid pcdna-tci. the attenuated s. enteritidis e23 was ...201121895998
late relapse of non-typhoidal salmonella vascular graft infection after 5 years.salmonella bacteremia can be complicated by mycotic aneurysm with the potential for a catastrophic presentation. treatment involves prompt surgery with debridement, extraanatomic bypass, and prolonged antibiotic therapy. any relapse tends to occur within the 1(st) year after surgery. we describe a case of salmonella enteritidis mycotic aneurysm in a 56-year-old man 5 years after the initial presentation, emphasizing the importance of aggressive initial therapy and long-term surveillance.201121885554
shift in chicken intestinal gene association networks after infection with salmonella.a primary infection of salmonella enteritidis causes a spatial-temporal dependent change in the gene expression patterns in the intestine of chickens (gallus gallus). this is the result of a dynamic intestinal response to adapt to the altered environment and to optimize its 'health' and functionality under the new circumstances. by inferring gene association networks (gans), the complexities of and changes in biological networks can be uncovered. within such gans highly interacting (hub) genes c ...201121885356
the stepone real-time polymerase chain reaction detection of salmonella sp., salmonella enterica ser. typhimurium and enteritidis in milk and meat.the aim of this study was to follow contamination of ready to eat milk and meat products with salmonella spp. by using the stepone real-time polymerase chain reaction (pcr). classical microbiological methods for detection of foodborne bacteria involve the use of pre-enrichment and/or specific enrichment, following isolation of bacteria in solid media and the final confirmation by biochemical and/or serological tests. we used the prepseq rapid spin sample preparation kit for isolation of dna and ...201121879831
A critical review of Salmonella Typhimurium infection in laying hens.Salmonella Typhimurium has been reported to contaminate egg production across the world, but where Salmonella Enteritidis is endemic it is this latter serovar that dominates egg-borne salmonellosis. However, Salmonella Typhimurium is a major food-borne pathogen so it is important to understand how it can impact the microbiological safety of eggs and what serovar-specific control strategies may be appropriate in the future as control over Salmonella Enteritidis continues to improve. To that end, ...201121879803
Standardisation of multilocus variable-number tandem-repeat analysis (MLVA) for subtyping of Salmonella enterica serovar Enteritidis.Salmonella enterica serovar (S.) Enteritidis is an important cause of food-borne infection in Europe and the United States. Further subtyping of isolates is necessary to support epidemiological data for the detection of outbreaks and identification of the vehicle of infection. Multilocus variable-number tandem-repeat analysis (MLVA) is reportedly more discriminatory and produces data that are easier to share via databases than other molecular subtyping methods. However, lack of standardisation o ...201121871223
The influence of the cage system and colonisation of Salmonella Enteritidis on the microbial gut flora of laying hens studied by T-RFLP and 454 pyrosequencing.In the EU conventional cages for laying hens are forbidden beginning in January 2012, however concerns about a higher transmission rate of Salmonella in alternative cages systems have been raised. The extent to which cage systems may affect the intestinal microbiota of laying hens is not known, and different microbiota may demonstrate different resistance towards colonization with Salmonella. To investigate this, ileal and caecal samples from two experimental studies where laying hens were inocu ...201121859465
Choose your menu wisely: cuisine-associated food-poisoning risks in restaurants in England and Wales.SUMMARYThe food service sector continues to be the most common setting for reported foodborne disease outbreaks in England and Wales. Using restaurant-associated foodborne outbreaks reported in England and Wales from 1992 to 2009, cuisine-specific risk factors were examined. Of 677 restaurant outbreaks, there were 11 795 people affected, 491 hospitalizations, and seven deaths; and Chinese, Indian, British and Italian cuisines were the most commonly implicated (26%, 16%, 13% and 10%, respectively ...201121854669
risk factors for salmonella prevalence in laying-hen farms in japan.summaryhuman salmonellosis cases, particularly those caused by salmonella enteritidis, have been closely linked to egg consumption. this epidemiological survey was conducted to determine the baseline salmonella prevalence and identify the risk factors for salmonella prevalence in laying-hen farms in japan. caecal excrement samples and dust samples were obtained from 400 flocks in 338 laying-hen farms. salmonella was identified in 20·7% of the farms and 19·5% of the flocks. the prevalence of salm ...201121849097
the inhibitory activity of lactobacillus spp. isolated from breast milk on gastrointestinal pathogenic bacteria of nosocomial origin.milk acts as a mean for transporting many essential substances from the mother to the child. in human beings, milk includes several predominant bacteria, such as staphylococci, streptococci, micrococci, lactobacilli, enterococci, lactococci and bifidobacteria. besides, its intake favors the predominance of bifidobacteria and lactobacilli in the child's intestinal microbiota. the present work explores the isolation and selection of lactobacilli strains with probiotic potential, focusing in their ...201121846506
anti-inflammatory properties of probiotic bacteria on salmonella-induced il-8 synthesis in enterocyte-like caco-2 cells.invasion of the gut by pathogenic salmonella leads to production of il-8 that initiates inflammatory reactions to combat the bacterium. however, its persistent production causes tissue damage and interventions that suppress il-8 production prevent tissue damage. we hypothesised that probiotics could mediate their benefits via inhibition of il-8 synthesis. caco-2 cells were infected with probiotic bifidobacterium infantis w52, lactobacillus casei w56, lactococcus lactis w58, lactobacillus acidoph ...201021840800
protective effect by bacillus smithii tbmi12 spores of salmonella serotype enteritidis in mice.avoiding food-borne diseases by competitive exclusion agents is a proactive strategy. in the current paper, we report the use of bacillus smithii tbmi12 spores as potential competitive exclusion agents. one group of mice was predosed for three successive days with 10(8) colony forming units of b. smithii tbmi12 spores followed by inoculation with 10(6) colony forming units of wild-type salmonella enterica serotype enteritidis cells. microbial plate counts of the animals' livers and spleens showe ...201021840796
occurrence of incfii plasmids carrying the bla(ctx-m-15) gene in salmonella enterica serovar enteritidis sequence type 11 in korea.a total of 49 consecutive nonduplicate salmonella isolates collected in a nationwide survey performed in 2009 in korea were included in this study. resistance gene, sizes, and replicon sequence types (rsts) of r plasmids, and sequence types (sts) and xbai-macrorestriction patterns of extended-spectrum +¦-lactamase (esbl)-producing isolates were determined. six salmonella enterica serovar enteritidis isolates of st11 showed esbl phenotypes, and the isolates harbored an incompatibility group fii p ...201121840672
adaptation to the chicken intestine in salmonella enteritidis pt4 studied by transcriptional analysis.the transcriptional changes that occurred in salmonella enterica serovar enteritidis during colonization of the alimentary tract of newly hatched chickens were studied. a whole genome oligonucleotide microarray was used to compare the expression pattern with that from bacteria cultured in nutrient broth in vitro. amongst other changes salmonella pathogenicity island (spi)-1, spi-2 and spi-5 genes were up-regulated in vivo suggesting a close association with the mucosa during colonization. partic ...201121831537
exploitation of intestinal colonization-inhibition between salmonella organisms for live vaccines in poultry - potential and limitations.immunization represents one of the most important methods to increase the resistance of chickens against salmonella infection. in addition to the development of an adaptive immune response, oral administration of live salmonella strains to day-old chicks provides protection against infection within hours by intestinal colonization-inhibition. for the exploitation of this phenomenon, practical information on colonization-inhibition between salmonella organisms is needed. colonization-inhibition c ...201121824353
engineering and pre-clinical evaluation of attenuated non-typhoidal salmonella strains serving as live oral vaccines and as reagent strains.while non-typhoidal salmonella (nts) have long been recognized as a cause of self-limited gastroenteritis, it is becoming increasingly evident that multiple antibiotic-resistant strains are also emerging as important causes of invasive bacteremia and focal infections, resulting in hospitalizations and deaths. we have constructed attenuated salmonella enterica serovar typhimurium and enteritidis strains that can serve as live oral vaccines and as "reagent strains" for subunit vaccine production i ...201121807911
salmonella enteritidis core-o polysaccharide (cops) conjugated to h:g,m flagellin as a candidate vaccine for protection against invasive infection with salmonella enteritidis.non-typhoidal salmonella serovars enteritidis and typhimurium are a common cause of gastroenteritis but also cause invasive infections and enteric fever in certain hosts (young children in sub-saharan africa, the elderly and immunocompromised). salmonella o polysaccharides (ops) and flagella proteins are virulence factors and protective antigens. the surface polysaccharides of salmonella are poorly immunogenic and do not confer immunologic memory, limitations overcome by covalently attaching the ...201121807909
effects of electron beam irradiation on the variability in survivor number and duration of lag phase of four food-borne organisms.the effect of electron beam irradiation on microbial inactivation and duration of lag time of individual surviving cells of listeria innocua, enterococcus faecalis, pseudomonas fluorescens and salmonella enteritidis has been studied. in addition, the data on variability in microbial inactivation and duration of lag phase for surviving microbes have been fitted by normal and gamma distributions, respectively. the standard deviations of survivor number and lag phase duration of individual cells we ...201121798613
comparison of real-time pcr with conventional pcr and culture to assess the efficacy of a live attenuated salmonella enterica serovar typhimurium vaccine against salmonella enterica serovar enteritidis in commercial leghorn chicks vaccinated under field and laboratory conditions.the efficacy of a live attenuated salmonella typhimurium megan vac 1 vaccine (mv1) was evaluated against salmonella enteritidis in chicken pullets with the use of pcr and culture methods. two hundred hyline w-32 white leghorn chicks were obtained from a local hatchery and divided into four treatment groups. two of the groups served as positive and negative controls. the mv1 vaccine was administered to the chicks in the remaining two groups at 1 and 35 days old by either the coarse spray (field) ...201121793441
the relationship between the numbers of salmonella enteritidis, salmonella heidelberg, or salmonella hadar colonizing reproductive tissues of experimentally infected laying hens and deposition inside eggs.contamination of eggs by salmonella enteritidis has been a prominent cause of human illness for several decades and is the focus of a recently implemented national regulatory plan for egg-producing flocks in the united states. salmonella heidelberg has also been identified as an egg-transmitted pathogen. the deposition of salmonella strains inside eggs is a consequence of reproductive tract colonization in infected laying hens, but prior research has not determined the relationship between the n ...201121793440
removal of radioisotopes in solution and bactericidal/bacteriostatic sterilising power in activated carbon and metal silver filters.activated carbon filters play an important role in water filtration and purification from contaminants of different origin. their limit consists in bacterial proliferation, which may occur only during prolonged periods of non-use and in their ability to remove radioactive contaminants present in waste water from industry or nuclear medicine departments. in this work we tested a commercially available activated carbon filter for water purification enriched with silver plated parts incubating in s ...200921783921
[antimicrobial activity of bacteriocin s760 produced by enterococcus faecium strain lwp760].antimicrobial activity of bacteriocin s760 (enterocin) produced by enterococcusfaecium strain lwp760 was studied. bacteriocin s760 is a cationic, hydrophobic, and heat stable peptide with the molecular weight of 5.5 kda and pl of 9.8. enterocin s760 is shown to inhibit in vitro the growth both of sensitive and resistant to antibacterials gramnegative and grampositive bacteria of 25 species. mics of the bacteriocin s760 vary between 0.05-1.6 mg/l for escherichia coli 0157:h117, salmonella typhimu ...201121780664
polymerase chain reaction amplification length-dependent ethidium monoazide suppression power for heat-killed enterobacteriaceae.the polymerase chain reaction (pcr) can confirm the presence of bacteria, but it is unable to differentiate between live and dead bacteria. although ethidium monoazide (ema)- and propidium monoazide (pma)-based pcr have been evaluated, a quantity of ?10(3)cells/ml dead cells produces a false-positive reading at 40 to 50cycles (k. rudi et al., appl. environ. microbiol. 71 (2005) 1018-1024). after confirming the precision of real-time pcr of a long dna target (16s or 23s ribosomal rna [rrna] gene, ...201121771573
salmonella enteritidis from a case of fever with thrombocytopenia.non typhoidal salmonella species are thought to be potentially infectious to humans. we isolated salmonella enteritidis from a 10-year-old boy with fever and thrombocytopenia. we reviewed the literature concerning infections caused by salmonella but we could not find any such case report from india.201121771481
in vitro antibacterial effect of aqueous and ethanolic moringa leaf extracts.to evaluate the antibacterial effect of aqueous and ethanolic moringa leaf extracts (moringa oleifera) on the growth of gram-positive and negative bacteria.201121771453
leukocytic responses and intestinal mucin dynamics of broilers protected with enterococcus faecium ef55 and challenged with salmonella enteritidis.the protective effect of enterococcus faecium ef55 in chickens challenged with salmonella enterica serovar enteritidis phage type 4 (se pt4) was assessed. the antibacterial effect on the bacterial microflora in the small intestine in relation to white blood cell count, phenotyping of peripheral blood and intestinal lymphocytes, functional activity of lymphocytes and phagocytes and mucin quantitation were investigated. day-old chicks (85) were randomly divided into four groups. the probiotic grou ...201121767856
effects of avian triggering receptor expressed on myeloid cells (trem-a1) activation on heterophil functional activities.a class of innate receptors called the triggering receptors expressed on myeloid cells (trem) has been discovered and shown to be involved in innate inflammatory responses. the trem family has been found in the chicken genome and consists of one activating gene (trem-a1) and two inhibitory genes (trem-b1 and trem-b2). however, to date, there have been no reports on the effects of activating the trem molecules on the functional activity of the primary avian polymorphonuclear cell, the heterophil. ...201121767563
evaluation of the colonization capabilities of salmonella enteritidis in quails using an rt-pcr approach.we used a real-time pcr assay and indirect fluorescent antibody (ifa) assay to detect genomic dna of salmonella enteritidis in the internal organs of quails after an oral challenge. the results showed that s. enteritidis was detected in all the samples at different time points. this study will assist a future understanding of the pathogenesis of s. enteritidis.201121764092
bacillus subtilis (dsm17299) significantly reduces salmonella in broilers.salmonella continues to be a major public health burden worldwide. poultry are known to be one of the main reservoirs for this zoonotic pathogen. it has previously been shown that a single dose of bacillus subtilis reduces fecal shedding of salmonella enterica serovar enteritidis, whereas no effect on long-term colonization of the cecum has been observed. here we report experiments that were undertaken to test the efficacy of a conventional diet supplemented with a probiotic (b. subtilis dsm1729 ...201121753205
microencapsulated sorbic acid and nature-identical compounds reduced salmonella hadar and salmonella enteritidis colonization in experimentally infected chickens.the reduction of salmonella prevalence in broilers is a priority in european union agricultural policies because treatment with antibiotics is forbidden by regulation (ec) 2160/2003. two trials were conducted to evaluate the efficacy of a microencapsulated blend of sorbic acid and nature-identical compounds (i.e., chemically synthesized botanicals; sab) on the reduction of the cecal prevalence and contents of salmonella enterica serovars hadar and enteritidis in experimentally infected chickens. ...201121753203
distinct lines of chickens express different splenic cytokine profiles in response to salmonella enteritidis challenge.chicken meat and eggs contaminated with salmonella result in economic losses in the poultry industry and potential human infection. intestinal parasites have been shown to lead to a reduction in the utilization of nutrients and performance in poultry. this study provides insight into the immune responses used by hens of 3 genetically distinct chicken lines (broiler, leghorn, and fayoumi) in the presence and absence of salmonella enteritidis infection. understanding the range of immune responses ...201121753200
biological activity of twenty-three hydantoin derivatives on intrinsic efflux pump system of salmonella enterica serovar enteritidis nctc 13349.hydantoin derivatives have important biochemical and pharmacological properties. in the present study, 23 hydantoin compounds were evaluated for their efflux-modulating effects in salmonella enterica serovar enteritidis nctc 13349 using real-time fluorimetry based on the intracellular accumulation of ethidium bromide (eb), a universal substrate of efflux pumps.201121753132
evaluation of forty new phenothiazine derivatives for activity against intrinsic efflux pump systems of reference escherichia coli, salmonella enteritidis, enterococcus faecalis and staphylococcus aureus strains.because phenothiazines inhibit efflux pumps of bacteria, forty new phenothiazine derivatives were tested for their inhibition of the efflux pump systems of gram-positive and gram-negative pathogenic bacteria.201121753124
incorporation of probiotic bacteria in whey cheese: decreasing the risk of microbial contamination.for dairy products that are consumed fresh, contamination by spoilage microorganisms and pathogens from the environment is a major concern. contamination has been associated with a number of outbreaks of foodborne illnesses; however, consistent data pertaining to the microbial safety of whey cheeses specifically have not been reported. hence, the goals of this research effort were (i) to manufacture a probiotic whey cheese with bifidobacterium animalis and lactobacillus casei and (ii) to assess ...201121740724
Refresh
firstprev
...11121314151617181920...
nextlast
Displaying items 1001 - 1100 of 6195