Publications

TitleAbstractYear
Filter
PMID(sorted descending)
Filter
metagenomic analysis of virulence-associated and antibiotic resistance genes of microbes in rumen of indian buffalo (bubalus bubalis).a major research goal in rumen microbial ecology is to understand the relationship between community composition and its function, particularly involved in fermentation process is of a potential interest. the buffalo rumen microbiota impacts human food safety as well as animal health. although the bacteria of bovine rumen have been well characterized, techniques have been lacking to correlate total community structure with gene function. we applied 454 next generations sequencing technology to c ...201222850272
molecular differentiation of peroxysome proliferator activated receptor coactivator-1 among different breeds of bubalus bubalis.peroxysome proliferator activated receptor coactivator-1 gene (ppargc1a) is a positional and functional candidate gene for milk fat yield. it has key role in energy, fat and glucose metabolism. single nucleotide polymorphisms (snps) in exon-8 of ppargc1a are reported to be associated with milk fat yield in dairy cattle. in the present investigation ppargc1a was partially amplified (around 767bp) by designing gene specific primer and confirm by sequencing the amplicon and its comparison with the ...201222829739
culture, characterization and differentiation of cells from buffalo (bubalus bubalis) amnion.stem cells present an important tool in livestock assisted reproduction and veterinary therapeutic field such as tissue engineering. we report for the first time isolation of pluripotent stem cell-like cells expressing pluripotency markers (alkaline phospahatase, oct-4, nanog and sox-2) from the amnion of water buffalo (bubalus bubalis). the cells showed no apparent abnormalities in their chromosomal profiles before and after cryopreservation. the cytochemical staining revealed that pluripotent ...201322820992
effect of parity, days in milk, and milk yield on detailed milk protein composition in mediterranean water buffalo.the effects of some nongenetic factors on milk protein fraction contents and relative proportions were estimated in 606 individual milk samples of mediterranean water buffalo. content of α(s1)-casein (cn), α(s2)-cn, β-cn, γ-cn, κκ-cn, glycosylated κ-cn (glyco-κ-cn), α-lactalbumin, and β-lactoglobulin was measured by reversed-phase hplc. relative contents of α(s1)-cn%, α(s2)-cn%, β-cn%, and κ-cn% were, respectively, 32.1, 17.1, 34.5, and 15.7%, whereas γ-cn% accounted for 0.6% of total casein con ...201222818435
a nonsense mutation in the tyrosinase gene causes albinism in water buffalo.oculocutaneous albinism (oca) is an autosomal recessive hereditary pigmentation disorder affecting humans and several other animal species. oculocutaneous albinism was studied in a herd of murrah buffalo to determine the clinical presentation and genetic basis of albinism in this species.201222817390
establishment and characterization of a buffalo (bubalus bubalis) mammary epithelial cell line.the objective of this study was to establish the buffalo mammary epithelial cell line (bumec) and characterize its mammary specific functions.201222792341
buffalo (bubalus bubalis) es cell-like cells are capable of in vitro skeletal myogenic differentiation.when buffalo embryonic stem (es) cell-like cells that expressed surface markers ssea-4, tra-1-60, tra-1-81, cd9 and cd90 and intracellular markers oct4, sox2 and foxd3, as shown by immunofluorescence, and that expressed rex-1 and nucleostemin as confirmed by rt-pcr, were subjected to suspension culture in hanging drops in absence of lif and buffalo foetal fibroblast feeder layer support, they differentiated to form three-dimensional embryoid bodies (ebs). of 231 ebs examined on day 3 of suspensi ...201322788718
serum acute phase proteins in control and theileria annulata infected water buffaloes (bubalus bubalis).this study was carried out to ascertain the changes in acute phase proteins (apps) and pro-inflammatory cytokines in theileria annulata infected water buffalo. thirty infected water buffaloes and 20 parasitologically free were used. in the present study there was significant (p ≤ 0.05) increase in haptoglobin (hp), serum amyloid a (saa), ceruloplasmin, α1-acid glycoprotein (agp) and fibrinogen levels (2.18 ± 0.29 g/l, 156.58 ± 3.48 mg/l, 31.23 ± 1.25mg/dl, 370.23 ± 33.21 mg/l and 16.17 ± 1.18 g/ ...201222785130
molecular cloning, sequence characterization and heterologous expression of buffalo (bubalus bubalis) oviduct-specific glycoprotein in e. coli.oviductin is a high molecular weight oviduct-specific glycoprotein secreted by the non-ciliated epithelial cells of oviduct during estrous cycle and early pregnancy. it plays an important role during fertilization and early embryonic development. the oviductin gene from oviductal tissues of buffalo was successfully cloned and sequenced. the sequence analysis revealed that buffalo and cattle oviductin share very high homology between their cdna sequences. the predicted amino acid sequences of the ...201222782592
tissue-specific temporal exome capture revealed muscle-specific genes and snps in indian buffalo (bubalus bubalis).whole genome sequencing of buffalo is yet to be completed, and in the near future it may not be possible to identify an exome (coding region of genome) through bioinformatics for designing probes to capture it. in the present study, we employed in solution hybridization to sequence tissue specific temporal exomes (tst exome) in buffalo. we utilized cdna prepared from buffalo muscle tissue as a probe to capture tst exomes from the buffalo genome. this resulted in a prominent reduction of repeat s ...201222768984
animal reservoirs of visceral leishmaniasis in india.visceral leishmaniasis (vl) is a disease that has both zoonotic and anthroponotic etiologies. in india, vl is endemic, considered to be anthroponotic, and caused by leishmania donovani . anthroponotic diseases are maintained by transmission from human to human and to a lesser extent from human to animals. serum samples from 1,220 animals from 7 human vl endemic districts of bihar, india, were tested for antibodies to a recombinant kinetoplast antigen (rk39 antigen) present in amastigotes of visc ...201322765517
prognosis prediction of uterine torsion mechanical treatment (rolling) after estimation of calcium and creatinine level in the serum of buffaloes (bubalus bubalis).the present study was carried out to investigate the relationship between creatinine and calcium concentration in buffalo serum in cases of uterine torsion before rolling, and 1 h and 24 h after calving. the degree, duration and site of uterine torsion, as well as fetus viability, time needed for cervical dilation, and the occurrence of uterine rupture were recorded. a total of 150 pregnant buffaloes suffering from colic and anorexia were brought to our clinic and clinically examined. of these, ...201222763076
collagen-iv supported embryoid bodies formation and differentiation from buffalo (bubalus bubalis) embryonic stem cells.embryoid bodies (ebs) are used as in vitro model to study early extraembryonic tissue formation and differentiation. in this study, a novel method using three dimensional extracellular matrices for in vitro generation of ebs from buffalo embryonic stem (es) cells and its differentiation potential by teratoma formation was successfully established. in vitro derived inner cell masses (icms) of hatched buffalo blastocyst were cultured on buffalo fetal fibroblast feeder layer for primary cell colony ...201222749767
isolation and enrichment of type a spermatogonia from pre-pubertal buffalo ( bubalus bubalis) testis.the aim of this study was to isolate and subsequently enrich type a spermatogonia from pre-pubertal buffalo testis. two-step enzymatic digestion was used to isolate spermatogonia from 10 to 14 months pre-pubertal buffalo calves, resulting in maximal release of spermatogonia from the seminiferous tubules. after enzymatic digestion, the type a spermatogonia were subsequently enriched by differential plating and percoll gradient centrifugation. the identity of type a spermatogonia was determined by ...201322742713
nutritional evaluation of organically grown fodders in lactating murrah buffaloes (bubalus bubalis).a study was undertaken to evaluate the nutritional response of organically grown diets in buffaloes on nutrients utilization and nutrient efficiency for milk production. for this, ten milch murrah buffaloes of average body weight (490.72 ± 6.65 kg), milk yield (8.13 ± 0.33 kg), and lactation stage (85 ± 5.28 days) were distributed in inorganic (indg()) and organic dietary groups (ordg) with five animals in each. buffaloes of indg and ogdg were fed sorghum hay-berseem fodder-concentrate mixture-b ...201322733348
molecular phylogeny of pakistani riverine buffalo based on genetic variability of mitochondrial cytochrome b gene.mitochondrial cytochrome b gene is considered to be one of the best markers for breed characterization as well as studying the ancestry in the vertebrates due to its exclusive maternal inheritance. dna fingerprinting by single nucleotide polymorphism is most reliable and widely used molecular technique in modern forensics and is being considered in this study. partial sequencing of 1,061 bp of aforementioned gene from 14580 to 15643 was conducted in two famous pakistani buffalo breeds named nili ...201222718511
isolation and characterization of bovine parainfluenza virus type 3 from water buffaloes (bubalus bubalis) in argentina.parainfluenza virus type 3 (piv3) was isolated from dairy buffaloes (bubalus bubalis) naturally affected with respiratory and reproductive clinical conditions.201222716217
chlamydiaceae in riverine buffalo (bubalus bubalis) and cows (bos taurus) in egypt with and without signs of reproductive disease.to obtain information and compare the prevalence of chlamydiaceae in riverine buffalo (bubalus bubalis) and cows (bos taurus) in egypt with and without clinical signs of reproductive disease.201222712774
sequence characterization of river buffalo toll-like receptor genes 1-10 reveals distinct relationship with cattle and sheep.the present study was undertaken to characterize the full-length transcripts of toll-like receptor (tlr) genes 1-10 of river buffalo. the conceptualized amino acid identity of bubaline tlrs ranged between 86% to 100% with ruminants, while it ranged between 45% to 91% with other vertebrate species. simple modular architecture tool (smart) analysis revealed the presence of tir domains and varying numbers of leucine-rich repeat motifs in all the buffalo tlrs. with respect to tir domains, tlrs 1, 2 ...201322694123
in vivo differentiation potential of buffalo (bubalus bubalis) embryonic stem cell.embryonic stem cells (escs) derived from inner cell mass (icm) of mammalian blastocyst are having indefinite proliferation and differentiation capability for any type of cell lineages. in the present study, icms of in vitro-derived buffalo blastocysts were cultured into two different culture systems using buffalo fetal fibroblast as somatic cell support and matrigel as synthetic support to obtain pluripotent buffalo embryonic stem cell (buesc) colonies. pluripotency of the escs were characterise ...201222678753
the effects of high temperature and roof modification on physiological responses of swamp buffalo (bubalus bubalis) in the tropics.the objective of the experiments reported here was to measure the effects of cooling techniques (modified roof vs normal roof) on the performance and physiology of 12 young male buffaloes with a similar live weight of 160 kg. the study was conducted at chainat agriculture and technology college, chainat province, thailand. the animals were divided randomly into two groups, each group comprising six buffaloes, and the two groups were studied to evaluate the effects of modified roofing (normal roo ...201322678302
advanced comparative cytogenetic analysis of x chromosomes in river buffalo, cattle, sheep, and human.based on a recently generated comprehensive gene map for ovis aries chromosome x (oarx) with an approximately even locus distribution, we assigned selected bacterial artificial chromosome (bac) probes corresponding to these oarx loci to bubalus bubalis (bbu) and bos taurus (bta) by comparative fluorescence in-situ hybridization (fish) to improve cytogenetically the x chromosome maps in these species. twenty-five added loci in bbux and btax, respectively, contribute to a more detailed description ...201222669522
natural trematode infection in liver of water buffalo (bubalus bubalis): histopathological investigation.this study reports the infection of liver and bile ducts, carried out from november 2008 to april 2010, on 730 randomly selected water buffaloes-bubalusbubalis, infected with the amphistome trematode parasite explanatum explanatum (creplin, 1847) fukui, 1929. macroscopic examination revealed massive infection of adult fluke in bile ducts and intrahepatic ductules in 131 (18%) cases. the predominant features were multifocal granulomatous nodules throughout the luminal surface of the bile ducts. h ...201122654314
construction and preliminary characterization of a river buffalo bacterial artificial chromosome library.river buffalo genome analyses have advanced significantly in the last decade, and the genome sequence of bubalus bubalis will be available shortly. nonetheless, large-insert dna library resources such as bacterial artificial chromosomes (bac) are still required for validation and accurate assembly of the genome sequence. we constructed a river buffalo bac library containing 52,224 clones with an average insert size of 97 kb, representing 1.7 × coverage of the genome. this genomic resource for ri ...201222653673
can rare positive interactions become common when large carnivores consume livestock?livestock populations in protected areas are viewed negatively because of their interaction with native ungulates through direct competition for food resources. however, livestock and native prey can also interact indirectly through their shared predator. indirect interactions between two prey species occur when one prey modifies either the functional or numerical responses of a shared predator. this interaction is often manifested as negative effects (apparent competition) on one or both prey s ...201222624309
sequence and topological characterization of toll-like receptor 8 gene of indian riverine buffalo (bubalus bubalis).in this study, buffalo (bubalus bubalis) toll-like receptor 8 (tlr8) gene has been characterized by sequence analysis and detecting polymorphism. complete orf of buffalo tlr8 gene was amplified using the rna isolated from spleen tissue, which was found to be 3,102 nucleotides long encoding a 1,033 amino acid protein. buffalo tlr8 had 10 nucleotide changes as compared to other livestock species resulting in six unique amino acid changes, four of them lying within leucine-rich repeat (lrr) domains ...201322622670
analysis of in vitro effects of sex steroids on lymphocyte responsiveness in murrah buffaloes (bubalus bubalis).present study was carried out on forty four apparently healthy murrah buffaloes of different age groups of both sexes to investigate the effects of sex steroids on cell mediated immunity in vitro. estrogen inhibited proliferation in mitogen-stimulated lymphocytes from prepubertal but not post pubertal buffaloes of either sex. estrogen at 100 pg/ml concentration stimulating the proliferation significantly (p < 0.05). in all groups and had higher stimulatory effect in lymphocytes from day 10 than ...201222619742
effect of csn1s1-csn3 (α(s1)-κ-casein) composite genotype on milk production traits and milk coagulation properties in mediterranean water buffalo.the aim of this study was to estimate effects of csn1s1-csn3 (α(s1)-κ-casein) composite genotypes on milk production traits and milk coagulation properties (mcp) in mediterranean water buffalo. genotypes at csn1s1 and csn3 and coagulation properties [rennet clotting time (rct), curd firming time (k₂₀), and curd firmness (a₃₀)] were assessed by reversed-phase hplc and computerized renneting meter analysis, respectively, using single test-day milk samples of 536 animals. alternative protein varian ...201222612978
gonadotropin-releasing hormone 1 directly affects corpora lutea lifespan in mediterranean buffalo (bubalus bubalis) during diestrus: presence and in vitro effects on enzymatic and hormonal activities.the expression of gonadotropin-releasing hormone (gnrh) receptor (gnrhr) and the direct role of gnrh1 on corpora lutea function were studied in mediterranean buffalo during diestrus. immunohistochemistry evidenced at early, mid, and late luteal stages the presence of gnrhr only in large luteal cells and gnrh1 in both small and large luteal cells. real-time pcr revealed gnrhr and gnrh1 mrna at the three luteal stages, with lowest values in late corpora lutea. in vitro corpora lutea progesterone p ...201222592497
uterine infection influences size and follicular fluid composition of the largest follicle in buffalo (bubalus bubalis).the effect of uterine infection on size and follicular fluid composition of the largest follicle was studied in buffalo. reproductive tracts were collected from 102 graded murrah buffaloes at an abattoir. uterine infection was diagnosed by physical examination of uterine mucus, white side test and uterine cytology. samples with pus-containing mucus, positive reaction on white side test and/or >5% neutrophils were considered to be positive for uterine infection. diameter of the largest follicle w ...201322583389
equivalency of buffalo (bubalus bubalis) embryonic stem cells derived from fertilized, parthenogenetic, and hand-made cloned embryos.this study was aimed at establishing buffalo embryonic stem cells (escs) from in vitro fertilized (ivf), parthenogenetic, and hand-made cloned (hmc) embryos and to check their equivalency in terms of stem cell marker expression, longevity, proliferation, and differentiation pattern. escs derived from all three sources were found by immunofluorescence to express the pluripotency markers ssea-4, tra-1-60, tra-1-81, oct4, and sox2 and were able to form embryoid bodies containing cells expressing ge ...201222582863
effects of plane of nutrition on slaughtering traits and meat characteristics in murrah graded male buffalo (bubalus bubalis) calves in nepal.an experiment was conducted using 17 male buffalo calves to assess the effects of plane of nutrition on slaughtering traits and meat characteristics. to attain 250 kg body weight (bw), the calves were allocated into three groups: high (h), low-high (l-h) and low (l) corresponding to concentrate levels receiving the concentrate at 1.50% of bw, 0.75% of bw until 190 kg bw and 1.50% thereafter, and 0.75% of bw, respectively. the animals had ad libitum access to urea-treated rice straw. no significa ...201122574796
characterization and in silico analysis of pregnancy-associated glycoprotein-1 gene of buffalo (bubalus bubalis).pregnancy-associated glycoproteins (pags) are trophoblastic proteins belonging to the aspartic proteinase family secreted by different placental cells of many mammalian species. they play a pivotal role in placentogenesis, foetomaternal unit remodeling, and implantation. the identification of the genes encoding those proteins will be helpful to unravel the intricate embryogenomic functions during pregnancy establishment. considering importance of these proteins, the present study was undertaken ...201122567354
power of exclusion of 19 microsatellite markers for parentage testing in river buffalo (bubalus bubalis).in the present study, 19 microsatellite markers were assessed for their power of exclusion to test parentage in river buffalo. microsatellite genotypes of 216 unrelated buffaloes belonging to five different breeds were utilized for the study. the probabilities of exclusion were calculated for three hypothetical situations viz. paternity testing (pe1), one parental genotype unavailable (pe2) and exclusion of both parents i.e. substituted offspring (pe3). the mean probability of exclusion across 1 ...201222555978
milk progesterone enzyme-linked immunosorbent assay as a tool to investigate ovarian cyclicity of water buffaloes in relation to body condition score and milk production.application of assisted reproductive technologies in buffaloes is limited to some extent by farmers' inability to detect oestrus because of its poor expression. the present study aimed at investigating reliability of a milk progesterone enzyme-linked immunosorbent assay (elisa) to assess the ovarian cyclicity during post partum, oestrus and post-breeding periods in water buffaloes.201222554129
effect of post-fusion holding time, orientation and position of somatic cell-cytoplasts during electrofusion on the development of handmade cloned embryos in buffalo (bubalus bubalis).the present study was conducted primarily to optimize electrofusion conditions for efficient production of zona-free nuclear transfer embryos in buffalos (bubalus bubalis). we found that 4v ac current for proper triplet alignment and single step fusion method, using a single dc pulse of 3.36 kv/cm for 4-μs duration, produced the most convincing results for efficient reconstitution of zona-free cloned embryos. lysis rate was very high (84.28 ± 2.59%) when triplets were in physical contact with ne ...201222541327
polymorphism and haplotype structure in river buffalo (bubalus bubalis) toll-like receptor 5 (tlr5) coding sequence.most of the 160 million river buffalo in the world are in asia where they are used extensively, both as a food source and for draught power. only recently have investigations begun exploring the buffalo genome for variation that might influence health and productivity of these economically important animals. this paper describes the sequence variability of the toll-like receptor 5 (tlr5) gene, which recognizes bacterial flagellin and is a key player in the immune system. tlr5 is comprised of a s ...201222537062
spermatogonial stem cells (sscs) in buffalo (bubalus bubalis) testis.water buffalo is an economically important livestock species and about half of its total world population exists in india. development of stem cell technology in buffalo can find application in targeted genetic modification of this species. testis has emerged as a source of pluripotent stem cells in mice and human; however, not much information is available in buffalo.201222536454
population structure and genetic variability in the murrah dairy breed of water buffalo in brazil accessed via pedigree analysis.the objective of this study was to use pedigree analysis to evaluate the population structure and genetic variability in the murrah dairy breed of water buffalo (bubalus bubalis) in brazil. pedigree analysis was performed on 5,061 animals born between 1972 and 2002. the effective number of founders (fe) was 60, representing 6.32 % of the potential number of founders. the effective number of ancestors (fa) was 36 and the genetic contribution of the 17 most influent ancestors explained 50 % of the ...201222535150
clinical and pathological insights into johne's disease in buffaloes.alternative diagnostic tools and interesting epidemiological assumptions were associated with an outbreak of johne's disease. in a buffalo herd infected with paratuberculosis, seven clinically affected animals and 21 animals with anti-mycobacterium avium elisa reactions were identified. total herd included 203 buffaloes. most lesions were comparable to those described in buffaloes and cattle affected by johne's disease. water buffalo behaviors such as communal nursing and allosuckling may be add ...201222528538
expression of transcriptional factor genes (oct-4, nanog, and sox-2) and embryonic stem cell-like characters in placental membrane of buffalo (bubalus bubalis).the aim of the study was to assess the expression of transcriptional factor genes and embryonic stem cell-like characters in the placental membrane of buffalo (bubalus bubalis). along with the placenta, amniotic fluid, maternal peripheral blood, and umbilical cord blood samples were taken for the future study. the isolation and culture of cells from the placental membrane was followed by the determination of rt-pcr-based markers (oct-4, nanog, sox-2, alkaline phosphatase, stem cell factor, and n ...201222527603
mitochondrial dna diversity patterns in pakistani buffalo.the indian subcontinent is considered to be the likely centre of river buffalo domestication, based on population dynamics, archaeological evidence and genetic diversity. recent studies on mitochondrial dna diversity have drawn useful conclusions about the domestication history of bubalus bubalis. the conclusions of these studies are, however, incomplete, unless samples can also be analysed from pakistan, which contains the second largest buffalo population of the world. here, we report the resu ...201122486503
molecular characterization of mhc-drb cdna in water buffalo (bubalus bubalis).in the present study, water buffalo mhc (bubu)-drb cdna was cloned and characterized. the 1022 base long-amplified cdna product encompassed a single open reading frame of 801 bases that coded for 266 amino acids. the bubu-drb sequence showed maximum homology with the bola-drb3*0101 allele of cattle. a total of seven amino acid residues were found to be unique for the bubu-drb sequence. the majority of amino acid substitutions was observed in the β(1) domain. residues associated with important fu ...201222481880
effect of l-arginine methyl ester (l-name) on hormonal profile and estrous cycle length in buffaloes (bubalus bubalis).the present study was undertaken to evaluate the effect of l-arginine methyl ester (l-name), a nitric oxide synthase inhibitor on serum nitric oxide, progesterone, estradiol profiles and estrous cycle length in buffaloes. murrah buffaloes (n = 16) exhibiting regular estrous cycles were randomly allocated to two groups of eight animals. in the treatment group, buffaloes were administered 400 mg/h l-name over 2 h (total dose = 800 mg) via the coccygeal artery and the aorta abdominalis on day 15 of ...201222476734
expression and characterization of constitutive heat shock protein 70.1 (hspa-1a) gene in in vitro produced and in vivo-derived buffalo (bubalus bubalis) embryos.cells are blessed with a group of stress protector molecules known as heat shock proteins (hsps), amongst them hsp70, encoded by hspa-1a gene, is most abundant and highly conserved protein. variety of stresses hampers the developmental competence of embryos under in vivo and in vitro conditions. present work was designed to study the quantitative expression of hspa-1a mrna in immature oocytes (imo), matured oocytes (mo), in vitro produced (ivp) and in vivo-derived (ivd) buffalo embryos to assess ...201222463675
embryonic genome activation events in buffalo (bubalus bubalis) preimplantation embryos.embryonic genome activation (ega) is the first major step towards successful initiation of preimplantation development, which culminates in the formation of implantation-competent embryos. ega occurs at species-specific embryonic cell stages. in the present work, ega was identified for buffalo embryos by studying the development rate of embryos in normal as well as imposed transcription block conditions, analyzing bromo-uridine triphosphate (brutp) incorporation rates as evidence of de novo tran ...201222461405
oxygen concentration and cysteamine supplementation during in vitro production of buffalo (bubalus bubalis) embryos affect mrna expression of bcl-2, bcl-xl, mcl-1, bax and bid.this study examined the effects of o(2) concentration (5% vs 20%) during in vitro maturation (ivm), fertilization (ivf) and culture (ivc) or supplementation of ivm and ivc media with cysteamine (50 and 100 μm, respectively; ivm, ivf and ivc carried out in 20% o(2)), on blastocyst rate and relative mrna abundance of some apoptosis-related genes measured by real-time qpcr in immature and in vitro-matured buffalo oocytes and in embryos at 2-, 4-, 8- to 16-cell, morula and blastocyst stages. the bla ...201222452597
influence of intergeneric/interspecies mitochondrial injection; parthenogenetic development of bovine oocytes after injection of mitochondria derived from somatic cells.interspecies/intergeneric mitochondrial heteroplasmy can occur in interspecies/intergeneric hybrid embryos or following nuclear transfer. in the present study, intergeneric buffalo (bubalus bubalis) mitochondria (wb-mt) or interspecies murine (mus spretus) mitochondria (m-mt) were injected into bovine (bos taurus) oocytes, and the subsequent embryonic development was characterized. fibroblast mitochondria (wb-mt or m-mt) were microinjected into in vitro matured bovine oocytes followed by oocyte ...201222447326
effect of spermine-nonoate on acrosome reaction and associated protein tyrosine phosphorylation in murrah buffalo (bubalus bubalis) spermatozoa.the present study was conducted to know the role of nitric oxide (no) on the acrosome reaction (ar) in murrah buffalo (bubalus bubalis) spermatozoa. ejaculated buffalo spermatozoa were washed, suspended in sp-talp media containing 6 mg bsa/ml and cell concentration was adjusted to 50×10(6) cells/ml. the cells were incubated for 6h in the absence or presence of heparin (10 μg/ml) to induce capacitation. fully capacitated spermatozoa were incubated in presence of 100 μg/ml lysophosphatidyl choline ...201222445612
identification and validation of reference genes for gene expression studies in water buffalo.in gene expression analysis, a key step to obtain informative data from reverse transcription quantitative pcr (rt qpcr) assay is normalization, that is usually achieved by ratio to correct the abundance of the gene of interest against that of an endogenous reference gene. the finding of such reference genes, ideally expressed in a stable way in multiple tissue samples and in different experimental conditions, is a non-trivial problem. in this work, a set of genes potentially useful as reference ...201022444257
effect of bromocryptine on hormones and milk secretion in murrah buffaloes ( bubalus bubalis).an experiment was carried out on 10 advance pregnant murrah buffaloes to determine the role of hormones in milk secretion around parturition. experimental animals were administered with a single injection of bromocryptine, @ 100 μg/kg bw, for 5 days before expected calving, whereas control group buffaloes were injected with placebo injections. blood samples collected before parturition (-5,-4,-3,-2,-1 days), on day of parturition (day-0) and on day 1, 2, 3, 4, 5, 10 and 15 post partum were analy ...201022444132
α-tocopherol and l-ascorbic acid increase the in vitro development of ivm/ivf swamp buffalo ( bubalus bubalis) embryos.this study was conducted to investigate the effects of capacitating agents added at in vitro fertilization (ivf) and antioxidants supplemented during in vitro culture (ivc) on the development of buffalo embryos. in experiment i, in vitro embryo development of buffalo embryos was compared when the ivf medium was supplemented with heparin, caffeine and calcium ionophore a23187 either alone or in combination. there was no significant difference (p > 0.05) in the cleavage rates of oocytes among the ...200822443906
characterization of osteopontin gene of bubalus bubalis.osteopontin, a glycoprotein, is expressed in several tissues including the mammary gland. the gene has been reported to be associated with milk and its constituents in various livestock species. this gene was sequenced in buffalo and it coded for the protein of 280 amino acids with the conserved grgds domain. the sequence was confirmed from the cdna sequence derived from the mammary gland of buffalo. the earlier-reported 9t/10t variation in the upstream region of the gene was investigated for it ...200822443697
the endocrine changes, the timing of ovulation and the efficacy of the doublesynch protocol in the murrah buffalo (bubalus bubalis).experiments were conducted to investigate (a) the timing of ovulation and the associated endocrine changes (progesterone, estrogen and lh) during estrous cycle and (b) the efficacy, with respect to the pregnancy rate, in cycling and anestrus in murrah buffaloes subjected to the doublesynch protocol during the low breeding season. in experiment 1, 10 cycling buffaloes were administered pgf(2α) on day 0 (without regard to the estrous cycle stage), gnrh on day 2, a second pgf(2α) injection on day 9 ...201222433942
generation of induced pluripotent stem cells from buffalo (bubalus bubalis) fetal fibroblasts with buffalo defined factors.ectopically, expression of defined factors could reprogram mammalian somatic cells into induced pluripotent stem cells (ipscs), which initiates a new strategy to obtain pluripotent stem cell lines. attempts have been made to generate buffalo pluripotent stem cells by culturing primary germ cells or inner cell mass, but the efficiency is extremely low. here, we report a successful method to reprogram buffalo fetal fibroblasts (bffs) into pluripotent stem cells [buffalo induced pluripotent stem ce ...201222420535
chromosome fragility in river buffalo cows exposed to dioxins.fifty river buffalo (bubalus bubalis, 2n = 50) cows reared in two different provinces of campania (southern italy) underwent cytogenetic investigations to ascertain possible differences in their chromosome stability. one group (caserta province) was under legal sequestration due to the presence in the milk mass of higher mean values of dioxins [21.79 pg/g of fat as sum of polychloro-dibenzo-dioxins (pcdds), polychloro-dibenzo-furans (pcdfs) and dioxin-like polychlorobiphenyls (dl-pcbs)] than bot ...201222415351
comparison of worm development and host immune responses in natural hosts of schistosoma japonicum, yellow cattle and water buffalo.yellow cattle and water buffalo are two of the most important natural hosts for schistosoma japonicum in china. previous observation has revealed that yellow cattle are more suited to the development of s. japonicum than water buffalo. understanding more about the molecular mechanisms involved in worm development, as well as the pathological and immunological differences between yellow cattle and water buffalo post infection with s japonicum will provide useful information for the vaccine design ...201222414188
improved rapd-pcr for discriminating breeds of water buffalo. 201222403004
inferring the phylogeny of bovidae using mitochondrial dna sequences: resolving power of individual genes relative to complete genomes.molecular techniques that assess biodiversity through the analysis of a small segment of mitochondrial genome have been getting wide attention for inferring the mammalian diversity. due to their highly conserved nature, specific mitochondrial genes offer a promising tool for phylogenetic analysis. however, there is no established criteria for selecting the typical mitochondrial dna (mtdna) segments to achieve a greater resolving power. we therefore chose the family bovidae as a model and compare ...201222399841
pcr-sscp analysis of leptin gene and its association with milk production traits in river buffalo (bubalus bubalis).leptin gene has been found to be associated with various economic traits including milk production and fat quality in dairy animals. in the present study, we investigated genetic variations in intron 1 region of leptin gene in riverine buffaloes (bubalus bubalis) using polymerase chain reaction-single strand conformation polymorphism (pcr-sscp) and sequencing methods and associated them with milk traits. the study revealed three sscp variants a, b and c among a total of 301 buffaloes from nine b ...201222395794
animal production systems of small farms in the kaski district of nepal.the aim of this study was to describe animal production on small farms in the kaski district of nepal, with the goal of identifying areas where animal health and productivity could be improved. eighty-five randomly selected farms from four different village development committees were visited. farmers were interviewed and premises and animals visually inspected on all farms. feed samples were collected from a subset of farms. the most commonly kept species were water buffalo (used for milk and m ...201222392548
effect of bypass fat supplementation on productive performance and blood biochemical profile in lactating murrah (bubalus bubalis) buffaloes.the study investigated the effect of dietary supplementation of bypass fat on productive performance and blood biochemical profile of lactating murrah buffaloes (bubalus bubalis). fifteen multiparous buffaloes (2-4 lactation) of early to mid lactation were divided in three homogenous groups t(1) (control), t(2), and t(3) of five each. the animals in t(1) were fed with a basal diet consisting of a concentrate mixture, green sorghum, and wheat straw as per requirements, while the animals in group ...201222373930
roscovitine treatment improves synchronization of donor cell cycle in g0/g1 stage and in vitro development of handmade cloned buffalo (bubalus bubalis) embryos.this study investigated the effects of serum-starvation, total confluence, and roscovitine treatment on cell-cycle synchronization of buffalo ear skin fibroblasts to the g0/g1 stage and on the developmental competence of cloned embryos. serum starvation of total confluence cultures for 24 h had a higher (p<0.05) proportion of cells at g0/g1 stage (94.4%) compared with serum starved cyclic and nonstarved confluent cultures (76.8 and 86.0%, respectively), whereas differences between cyclic cells w ...201222372577
short communication: prevalence and risk factors of subclinical mastitis as determined by the california mastitis test in water buffaloes (bubalis bubalis) in nueva ecija, philippines.a retrospective analysis using records of lactating bulgarian murrah buffaloes subjected to the california mastitis test in a herd in nueva ecija, philippines was done to determine the prevalence of subclinical mastitis (scm) and to identify risk factors that may influence its occurrence and recurrence. results showed that scm prevalence was 42.76%, whereas its recurrence was 75.03%. age and lactation length influenced the occurrence of scm. in contrast to the conclusions for dairy cows, younger ...201222365218
effect of incorporation of additives in tris-based egg yolk extender on buffalo (bubalus bubalis) sperm tyrosine phosphorylation during cryopreservation.phosphorylation of tyrosine residues on sperm protein is a known indicator of capacitation and a major intracellular signalling event. there is evidence that sperm cryopreservation promotes tyrosine phosphorylation and is associated with reduced fertility of spermatozoa. under this study, cryoprotective role of different additives namely taurine, trehalose, catalase and 4-bromophenacyl bromide on buffalo sperm quality was evaluated. buffalo semen was cryopreserved in tris-based egg yolk extender ...201122364363
phenotype characterization of staphylococcus species strains isolated from buffalo (bubalus bubalis) milk.the objective of the current study was to isolate and identify phenotypes of staphylococcus spp. strains derived from buffalo (bubalus bubalis) milk. a total of 548 milk samples from 137 buffalo were cultured in columbia agar enriched with 5% defibrinated sheep blood. determination of the capacity of staphylococcus aureus to produce enterotoxins a-d and toxic shock syndrome toxin-1 (tsst-1) was achieved by reverse passive latex agglutination (rpla). antimicrobial sensitivity of s. aureus strains ...201122362803
microbial population in the rumen of swamp buffalo (bubalus bubalis) as influenced by coconut oil and mangosteen peel supplementation.four, rumen fistulated swamp buffalo bulls were used to study microbial populations in the rumen when supplemented with coconut oil and mangosteen peel. animals were randomly assigned to a 4 × 4 latin square design. four treatments were un-supplemented (control), supplementation with coconut oil at 50 g/kg (co5), supplementation with mangosteen peel at 30 g/kg (mp3) and supplementation with co5 and mp3 (com), of total dm intake. animals received concentrate at 10 g/kg of bw, and rice straw was g ...201322356106
the past and present of and potential for the domestic (water) buffalo in africa.egypt is the only country in africa where domestic buffalo have a long-term presence and constitute an important part of the array of domestic animal resources. attempts to introduce buffalo to other african countries have been made since at least the 1920s. nine such attempted introductions are documented in this paper, although for most cases, there is very limited information. buffalo have disappeared without trace in some countries and were slaughtered in at least two because of lack of adap ...201222327409
pregnancy-specific protein b (pspb), progesterone and some biochemical attributes concentrations in the fetal fluids and serum and its relationship with fetal and placental characteristics of iraqi riverine buffalo (bubalus bubalis).this study was carried out to demonstrate the pregnancy-specific protein b (pspb), progesterone and some biochemical parameters concentrations in amniotic fluid, allantoic fluid and fetal serum collected from slaughtered iraqi riverine pregnant buffaloes at three different months of gestation (6th, 7th and 8th). ten out of 22 adult buffaloes of 4.6 ± 0.97 years old were used in this study. the buffaloes were mated naturally by monitoring the estrus cycles via appearance of vaginal fluids and mou ...201222325967
serological and bacteriological responses of water buffalo (bubalus bubalis) vaccinated with two doses of brucella abortus strain rb51 vaccine.thirty-two water buffalo (bubalus bubalis) calves aged 6–10 months were used to evaluate serological responses to brucella abortus strain rb51 (rb51) vaccination in a dose-response study and to compare the use of two selective media for the isolation of rb51. the animals were randomly divided into three treatment groups. groups i-iii received the recommended vaccine dose (rd) twice 4 weeks apart, rd twice 18 weeks apart and saline once, respectively. lymph nodes were excised from the three group ...201222297422
analysis of rumen methanogen diversity in water buffaloes (bubalus bubalis) under three different diets.the water buffalo (bubalus bubalis) is a prominent livestock species for the production of milk and meat in many countries. we investigated the diversity of rumen methanogens in mediterranean water buffaloes maintained in brazil under different diets: corn silage, grazing pasture, or sugar cane. a total of 467 clones were isolated from three methanogen 16s rrna gene clone libraries that each represented a distinct feed type. the 467 clones were assigned to 19 species-level operational taxonomic ...201222286379
serological profile of buffalo (bubalus bubalis) female calves vaccinated with standard brucella abortus strain 19 vaccine using rose bengal, 2-mercaptoethanol and complement fixation tests.the serological profiles of 21 female buffaloes vaccinated between 3 and 8 months of age using brucella abortus strain 19 (s19) were evaluated by rose bengal (rbt), 2-mercaptoethanol (2me) and complement fixation (cft) tests. the serum strains were collected in day zero, 15, 30, 45, 60th days and subsequently to each 30 months, until 720th day after vaccination. no animal showed reaction in day zero. in 15th day above 95% of animals revealed reaction in all tests. all the animals presented absen ...201222284623
an association analysis between oxt genotype and milk yield and flow in italian mediterranean river buffalo.the aim of this study was to evaluate possible associations between three snps at the oxytocin locus (am234538: g.28c>t; g.204a>g and g.1627g>t) and two productive traits, milk yield and milkability, in italian mediterranean river buffaloes. effects of parity, calving season and month of production were also evaluated. a total of 41 980 test-day records belonging to 219 lactations of 163 buffalo cows were investigated. the allele call rate was 98·8% and the major allele frequency for all the inv ...201222280971
acp5 (uteroferrin): phylogeny of an ancient and conserved gene expressed in the endometrium of mammals.type 5 acid phosphatase (acp5; also known as tartrate-resistant acid phosphatase or uteroferrin) is a metalloprotein secreted by the endometrial glandular epithelium of pigs, mares, sheep, and water buffalo. in this paper, we describe the phylogenetic distribution of endometrial expression of acp5 and demonstrate that endometrial expression arose early in evolution (i.e., before divergence of prototherian and therian mammals ~166 million years ago). to determine expression of acp5 in the pregnan ...201222278982
alterations in follicular fluid estradiol, progesterone and insulin concentrations during ovarian acyclicity in water buffalo (bubalus bubalis).ovarian acyclicity is one of the most important causes of infertility in water buffalo. recent studies have indicated alterations in the composition of follicular fluid during the condition. the aim of this study was to determine the changes in follicular fluid concentrations of estradiol, progesterone and insulin during ovarian acyclicity in water buffalo. ovaries were collected from 50 acyclic and 95 cyclic (control) buffaloes and follicular fluid was aspirated from small (5.0-6.9 mm), medium ...201222277844
co-culture of buffalo (bubalus bubalis) preantral follicles with antral follicles: a comparative study of developmental competence of oocytes derived from in vivo developed and in vitro cultured antral follicles.the present study was undertaken to examine whether the presence of antral follicles (afs) affects the survival, growth and steroidogenesis of preantral follicles (pfs) and compare the maturation and developmental competence of buffalo oocytes derived from in vivo developed and in vitro cultured afs. two experiments were carried out. in experiment i, pfs (200-250 μm) were isolated and cultured with or without afs (3-5 mm) in tcm-199 medium that contained 10% fetal bovine serum (fbs), 1% insulin ...201322251772
effects of plane of nutrition on growth, feed intake, digestibility and nitrogen balance in murrah graded male buffalo (bubalus bubalis) calves in nepal.an experiment was conducted using 17 male buffalo calves to assess the effects of plane of nutrition on dry matter intake (dmi), daily gain (dg), body size measurement, apparent digestibility and nitrogen (n) balance. to attain 250 kg bw, the calves were allocated into three groups: h, l-h and l, receiving the concentrate at 1.50% of bw, 0.75% of bw until 190 kg bw and 1.50% thereafter and 0.75% of bw, respectively. the animals had ad libitum access to urea-treated rice straw (utrs). the dmi of ...201222250739
experimental inoculation of neospora caninum in pregnant water buffalo.the aim of this study was to characterize the pathogenesis of neospora caninum in experimentally inoculated pregnant water buffalo (bubalus bubalis). twelve mediterranean female water buffaloes ranging in age from 4 to 14 years old and seronegative to n. caninum by indirect fluorescent antibody test (ifat) were involved. ten females were intravenously inoculated with 10(8) tachyzoites of nc-1 strain at 70 (n=3) or 90 (n=7) days of pregnancy (dp). two control animals were inoculated with placebo ...201122244534
pharmacokinetics and relative bioavailability of praziquantel in healthy water buffalo after oral and intramuscular administration. 201222239339
production of nuclear transfer embryos by using somatic cells isolated from milk in buffalo (bubalus bubalis).somatic cells in milk are a potential source of nuclei for nuclear transfer to produce genetically identical animals; this is especially important in animals that are susceptible to risks of bacterial infection on biopsy collection. in this study, a minimum of 10 milk samples were collected from each of the three buffaloes representing murrah breed. all the samples were processed immediately and cell colonies were obtained. cell colonies from one buffalo (mu-442) survived beyond 10 passages and ...201222229797
lacking expression of paternally-expressed gene confirms the failure of syngamy after intracytoplasmic sperm injection in swamp buffalo (bubalus bubalis).the objectives were to: (1) examine the efficiency of intracytoplasmic sperm injection (icsi) technique, with or without chemical activation of in vitro matured buffalo oocytes, on sperm head decondensation; and (2) compare the subsequent development of embryos after activation of icsi (icsi (+) activation group) and sham injection (sham (+) activation group) oocytes (embryos obtained by in vitro fertilization of ivm oocytes served as a control group). pronuclear formation rates in icsi (+) acti ...201222225682
characterization of prlr and ppargc1a genes in buffalo (bubalus bubalis).more than 40 million households in india depend at least partially on livestock production. buffaloes are one of the major milk producers in india. the prolactin receptor (prlr) gene and peroxisome proliferators activated receptor-γ coactivator 1-alpha (ppargc1a) gene are reportedly associated with milk protein and milk fat yields in bos taurus. in this study, we sequenced the prlr and ppargc1a genes in the water buffalo bubalus bubalis. the prlr and ppargc1a genes coded for 581 and 819 amino ac ...201122215963
soya-lecithin in extender improves the freezability and fertility of buffalo (bubalus bubalis) bull spermatozoa.egg yolk is routinely used as a cryoprotectant in semen extenders. however, it may contain cryoprotective antagonists, and there are hygienic risks associated with its use. proteins of plant origin, like soya-lecithin, lack these hazards. the aim of this study was to use soya-lecithin as a cryoprotectant in extender and to investigate its effects on in vitro quality and in vivo fertility of buffalo semen. semen from three buffalo bulls was frozen in tris-citric extender containing 5.0%, 10% or 1 ...201122211278
evaluation of blood antioxidant defense and apoptosis in peripheral lymphocytes on exogenous administration of pineal proteins and melatonin in rats.in view of the significant health impact of oxidative stress and apoptosis dysfunction, and further, because of suggestions that administration of antioxidants might reduce apoptosis rate through up-regulation of body antioxidant defense systems, therefore the purpose of this study was to compare the effect of buffalo (bubalus bubalis) pineal proteins (pp at 100 μg/kg bw, i.p.) with melatonin (mel at 10 mg/kg bw, i.p.) on blood (erythrocytes) antioxidant defense system and apoptosis in isolated ...201122205582
Frequency of Toxoplasmosis in Water Buffalo (Bubalus bubalis) in Trinidad.Toxoplasmosis has been reported to occur in several animals and humans causing different clinical manifestations. The study was conducted to determine the frequency of Toxoplasma gondii antibodies (IgG) in water buffalo (Bubalus bubalis) across farms in Trinidad using a latex agglutination test. Of a total of 333 water buffalo tested, 26 (7.8%) were seropositive for T. gondii antibodies. Seropositivity for toxoplasmosis was statistically significantly (P < 0.05; ?(2)) higher in adult water buffa ...201122195295
Fermentation kinetics of sainfoin hay with and without PEG.The in vitro gas production technique was applied to evaluate the biological activity of tannins in sainfoin (Onobrychis viciifolia Scop.) hay cut at four progressive phenological stages. The buffalo (Bubalus bubalis) rumen fluid was used as inoculum, polyethylene glycol (PEG) was added as specific binding agent and an alfalfa hay (AH) was used as control. All samples were analysed for chemical composition and condensed tannin (CTs). According to the phenological stage of sainfoin, crude protein ...201122168179
structure-activity relationship of buffalo antibacterial hepcidin analogs.hepcidin is an anti-microbial peptide expressed predominantly in the liver of many species. based on the amino acid sequence deduced from buffalo (bubalus bubalis) hepcidin cdna (accession no. eu399814), six peptides hepc(1-25), hepc(6-25), hepc(7-25), hepc(9-25), hepc(11-25) and hepc(15-25) were synthesized using solid-phase fluorenylmethoxycarbonyl (fmoc) chemistry. cd spectroscopy revealed different spectra of the peptides in different solvents and in all the cases beta-structure was found to ...201122165290
Diversity analysis of methanogens in rumen of Bubalus bubalis by 16S riboprinting and sequence analysis.The molecular diversity of rumen methanogens was investigated by 16S rDNA gene library prepared from the rumen contents obtained from Murrah buffaloes in India. Genomic DNA was isolated from adult male fistulated buffaloes and PCR conditions were set up using specific primers. Amplified product was cloned into a suitable vector, and the positive clones were selected assuming based on blue-white screening and sequenced. Positive clones were reamplified and the resulting PCR products were further ...201222155312
occurrence of theileria and babesia species in water buffalo (bubalus babalis, linnaeus, 1758) in the hubei province, south china.the presence and prevalence of tick-borne haemoparasites in water buffalo from the hubei province, south china was investigated using the reverse line blot (rlb) hybridization assay and phylogenetic analysis of the parasite 18s rrna gene. theileria buffeli (19.1%) was the most frequently found species in all of the locations, followed by babesia orientalis (8.9%), babesia bovis (1.0%) and babesia bigemina (0.7%). only 12 (3.9%) of the samples had mixed infections. eleven samples with single infe ...201122154255
cloning and sequencing of indian water buffalo (bubalus bubalis) interleukin-3 cdna.full-length cdna (435 bp) of the interleukin-3(il-3) gene of the indian water buffalo was amplified by reverse transcriptase-polymerase chain reaction and sequenced. this sequence had 96% nucleotide identity and 92% amino acid identity with bovine il-3. there are 10 amino acid substitutions in buffalo compared with that of bovine. the amino acid sequence of buffalo il-3 also showed very high identity with that of other ruminants, indicating functional cross-reactivity. structural homology modell ...201122152058
evaluation of indirect tasp enzyme-linked immunosorbent assay for diagnosis of tropical theileriosis in cattle (bos indicus) and water buffaloes (bubalus bubalis) in egypt.the aim of the present study was to evaluate the validity of theileria annulata surface protein (tasp)-elisa, in comparison with traditional microscopic test, for the diagnosis of t. annulata infection among egyptian baladi cattle (bos taurus) and water buffaloes (bubalus bubalis). molecular confirmation of infection using t. annulata merozoite surface (tams-1) target amplification by pcr was used as a gold standard. a total of 76 clinically suspected animals including 64 baladi cattle and 12 wa ...201122136769
a possible case of caprine-associated malignant catarrhal fever in a domestic water buffalo (bubalus bubalis) in switzerland.abstract:201122132808
comparative proteomics to evaluate multi drug resistance in escherichia coli.drug resistance in food-borne bacterial pathogens is an almost inevitable consequence of the use of antimicrobial drugs, used either therapeutically or to avoid infections in food-producing animals. in the past decades, the spread and inappropriate use of antibiotics have caused a considerable increase of antibiotics to which bacteria have developed resistance and, moreover, bacteria are becoming resistant to more than one antibiotic simultaneously. understanding mechanisms at the molecular leve ...201122120138
molecular cloning and characterization of th1 and th2 cytokines of african buffalo (syncerus caffer).the african buffalo (syncerus caffer) has been implicated as the reservoir of several bovine infectious agents. however, there is insufficient information on the protective immune responses in the african buffalo, particularly in infected animals. in this study, we analysed th1 cytokines il-2 and ifn-γ, and th2 cytokines il-4 and il-10. the cloned cdna of il-2, il-4, il-10 and ifn-γ contained an open reading frame of 468, 501, 408 and 540 nucleotides, encoding polypeptides of 155, 166, 135 and 1 ...201122117600
evaluation of fasting metabolism of growing water buffalo (bubalus, bubalis).the objectives of the present study were to evaluate fasting metabolism (fm) of water buffalo (bubalus, bubalis) at three stages of growth (12, 18 and 24 months) in guangxi, china. five female water buffalo were used for each age group and their live weight was on average 254, 326 and 338 kg, respectively. all animals were of average body condition, healthy and de-wormed before start of the study. prior to a 6-day fasting period, buffalo were offered a mixed diet of forage and concentrates (70% ...201122111628
derivation, characterization and differentiation of buffalo (bubalus bubalis) amniotic fluid derived stem cells.amniotic fluid cells (afcs) are obtained from amnion for pre-natal analysis and can be cultured in vitro. heterogeneous amniotic fluid (af) contains various cell types, and it is believed that some of these cells possess the stem cell properties. the aim of this study was to characterize these cells by phenotypical and genotypical means in buffalo. the differentiation potential of amniotic fluid stem (afs) cells was carried out by converting these cells into neurons. the afcs were cultured witho ...201122107067
Buffalo (Bubalus bubalis) Epiphyseal Proteins Counteract Arsenic-Induced Oxidative Stress in Brain, Heart, and Liver of Female Rats.Arsenic (As) toxicity through induction of oxidative stress is a well-known mechanism of organ toxicity. To address this problem, buffalo epiphyseal proteins (BEP, at 100 µg/kg BW, i.p. for 28 days) were administered intraperitoneally to female Wistar rats exposed to As (100 ppm sodium arsenite via drinking water for 28 days). Arsenic exposure resulted in marked elevation in lipid peroxidation in brain, cardiac, and hepatic tissues, whereas significant (p?<?0.05) adverse change in catalase, supe ...201122095291
some qualitative and chromatic aspects of thawed buffalo (bubalus bubalis) meat.after thawing, the meat of beef calves (italian frisian breed) and buffalo calves (mediterranean breed) slaughtered at 4, 8 and 12 months of age was examined. both the ph and the thawing loss confirmed that the meat of buffalo calves is more suitable for preservation by freezing. with increased age and time of exposure to air the lightness of the non-renewed surface was reduced. the lightness of the fresh cut surface remained stable in the various thawing phases though it was less in the older a ...200722064306
effectiveness of poll stunning water buffalo with captive bolt guns.practical experience has indicated that shooting water buffalo with a captive bolt gun in the front of the head does not always produce an effective stun. slaughtermen have been claiming that the poll position is more reliable, but under present eu regulations this shooting position is not allowed for domesticated bovines. this study examined the effectiveness of shooting water buffalo with captive bolt guns in the poll region. the depth of concussion was assessed in 30 water buffalo from physic ...200922063979
difference in tenderness and ph decline between water buffalo meat and beef during postmortem aging.the objective of this research was to determine the difference in tenderness and some characteristics of water buffalo meat and beef during postmortem aging. five female crossbred water-buffalo (philippine carabao×bulgarian murrah) and five female crossbred cattle (brahman×philippine native), were finished on the same diet for 6 months and slaughtered at 30 months of age. the muscle ph was measured at 40min, 3h, 7h, 24h, and 48h postmortem. longissimus thoracis (lt) and semimembranosus (sm) musc ...200722063807
Refresh
firstprev
...11121314151617181920...
nextlast
Displaying items 1001 - 1100 of 2518