Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| molecular identification of salmonella enterica subsp. enterica serovar gallinarum biovars gallinarum and pullorum by a duplex pcr assay. | salmonella enterica subsp. enterica serovar gallinarum biovar gallinarum (s gallinarum) and biovar pullorum (s pullorum) are 2 poultry pathogens that cause major economic losses to the poultry industry worldwide. control of both diseases mainly relies on the adoption of biosecurity programs, and success is dependent on accurate and fast detection. based on this concept, we developed a duplex pcr assay, targeting 2 chromosomal sequences, which allowed us to precisely identify and differentiate s ... | 2016 | 27216724 |
| a centrifugal direct recombinase polymerase amplification (direct-rpa) microdevice for multiplex and real-time identification of food poisoning bacteria. | in this study, we developed a centrifugal direct recombinase polymerase amplification (direct-rpa) microdevice for multiplex and real-time identification of food poisoning bacteria contaminated milk samples. the microdevice was designed to contain identical triplicate functional units and each unit has four reaction chambers, thereby making it possible to perform twelve direct-rpa reactions simultaneously. the integrated microdevice consisted of two layers: rpa reagents were injected in the top ... | 2016 | 27216297 |
| serum bactericidal assay for the evaluation of typhoid vaccine using a semi-automated colony-counting method. | typhoid fever, mainly caused by salmonella enterica serovar typhi (s. typhi), is a life-threatening disease, mostly in developing countries. enzyme-linked immunosorbent assay (elisa) is widely used to quantify antibodies against s. typhi in serum but does not provide information about functional antibody titers. although the serum bactericidal assay (sba) using an agar plate is often used to measure functional antibody titers against various bacterial pathogens in clinical specimens, it has rare ... | 2016 | 27216239 |
| characterization of a small outbreak of salmonella enterica serovar infantis that harbour ctx-m-65 in ecuador. | 2017 | 27215783 | |
| serodiagnosis of acute typhoid fever in nigerian pediatric cases by detection of serum iga and igg against hemolysin e and lipopolysaccharide. | inexpensive, easy-to-use, and highly sensitive diagnostic tests are currently unavailable for typhoid fever. to identify candidate serodiagnostic markers, we have probed microarrays displaying the full salmonella enterica serovar typhi (s. typhi) proteome of 4,352 different proteins + lipopolysaccharides (lpss), with sera from nigerian pediatric typhoid and other febrile cases, nigerian healthy controls, and healthy u.s. adults. nigerian antibody profiles were broad (∼500 seropositive antigens) ... | 2016 | 27215295 |
| phenotype microarray analysis of the drug efflux systems in salmonella enterica serovar typhimurium. | a large number of drug efflux transporters have been identified in salmonella enterica serovar typhimurium, and increased expression of these transporters confers drug resistance in this organism. here we compared the respiration activities of the wild-type strain and a mutant with nine deleted transporters by phenotype microarray analysis. the mutant was susceptible to 66 structurally unrelated compounds including many antibiotics, dyes, detergents, antihistamine agents, plant alkaloids, antide ... | 2016 | 27210311 |
| detection of zoonotic enteropathogens in children and domestic animals in a semirural community in ecuador. | animals are important reservoirs of zoonotic enteropathogens, and transmission to humans occurs more frequently in low- and middle-income countries (lmics), where small-scale livestock production is common. in this study, we investigated the presence of zoonotic enteropathogens in stool samples from 64 asymptomatic children and 203 domestic animals of 62 households in a semirural community in ecuador between june and august 2014. multilocus sequence typing (mlst) was used to assess zoonotic tran ... | 2016 | 27208122 |
| flashy flagella: flagellin modification is relatively common and highly versatile among the enterobacteriaceae. | post-translational glycosylation of the flagellin protein is relatively common among gram-negative bacteria, and has been linked to several phenotypes, including flagellar biosynthesis and motility, biofilm formation, host immune evasion and manipulation and virulence. however to date, despite extensive physiological and genetic characterization, it has never been reported for the peritrichously flagellate enterobacteriaceae. | 2016 | 27206480 |
| rflm mediates target specificity of the rcscdb phosphorelay system for transcriptional repression of flagellar synthesis in salmonella enterica. | the bacterial flagellum enables directed movement of salmonella enterica towards favorable conditions in liquid environments. regulation of flagellar synthesis is tightly controlled by various environmental signals at transcriptional and post-transcriptional levels. the flagellar master regulator flhd4 c2 resides on top of the flagellar transcriptional hierarchy and is under autogenous control by flhd4 c2 -dependent activation of the repressor rflm. the inhibitory activity of rflm depends on the ... | 2016 | 27206164 |
| effect of reduced graphene oxide-hybridized zno thin films on the photoinactivation of staphylococcus aureus and salmonella enterica serovar typhi. | the immobilization of photocatalyst nanoparticles on a solid substrate is an important aspect for improved post-treatment separation and photocatalyst reactor design. in this study, we report the simple preparation of reduced graphene oxide (rgo)-hybridized zinc oxide (zno) thin films using a one-step electrochemical deposition, and investigated the effect of rgo-hybridization on the photoinactivation efficiency of zno thin films towards staphylococcus aureus (s. aureus) and salmonella enterica ... | 2016 | 27203568 |
| virulence characterisation of salmonella enterica isolates of differing antimicrobial resistance recovered from uk livestock and imported meat samples. | salmonella enterica is a foodborne zoonotic pathogen of significant public health concern. we have characterized the virulence and antimicrobial resistance gene content of 95 salmonella isolates from 11 serovars by dna microarray recovered from uk livestock or imported meat. genes encoding resistance to sulphonamides (sul1, sul2), tetracycline [tet(a), tet(b)], streptomycin (stra, strb), aminoglycoside (aada1, aada2), beta-lactam (bla tem), and trimethoprim (dfra17) were common. virulence gene c ... | 2016 | 27199965 |
| regulation of the two-component regulator cpxr on aminoglycosides and β-lactams resistance in salmonella enterica serovar typhimurium. | the two-component signal transduction system cpxar is especially widespread in gram-negative bacteria. it has been reported that cpxar contributes to the multidrug resistance (mdr) in escherichia coli. cpxr is a response regulator in the two-component cpxar system. the aim of this study was to explore the role of cpxr in the mdr of s. enterica serovar typhimurium. the minimal inhibitory concentrations (mics) of various antibiotics commonly used in veterinary medicine for strains js (a multidrug- ... | 2016 | 27199934 |
| the small rna dsra influences the acid tolerance response and virulence of salmonella enterica serovar typhimurium. | the gram-negative, enteropathogen salmonella enterica serovar typhimurium (s. typhimurium) is exposed to various stress conditions during pathogenesis, of which acid stress serves as a major defense mechanism in the host. such environments are encountered in the stomach and salmonella containing vacuole of phagocytic and non-phagocytic cells. it is only recently that small rnas (srnas) have come to the forefront as major regulators of stress response networks. consequently, the srna dsra which r ... | 2016 | 27199929 |
| mechanisms of salmonella typhi host restriction. | salmonella enterica serovar typhi (s. typhi) is the cause of typhoid fever, a life-threatening bacterial infection that is very common in the developing world. recent spread of antimicrobial resistant isolates of s. typhi makes typhoid fever, a global public health risk. despite being a common disease, still very little is known about the molecular mechanisms underlying typhoid fever and s. typhi pathogenesis. in contrast to other salmonellae, s. typhi can only infect humans. the molecular bases ... | 2016 | 27193549 |
| competitive survival of escherichia coli, vibrio cholerae, salmonella typhimurium and shigella dysenteriae in riverbed sediments. | studies on the survival of bacterial enteric pathogens in riverbed sediments have mostly focused on individual organisms. reports on the competitive survival of these pathogens in riverbed sediments under the same experimental setup are limited. we investigated the survival of escherichia coli, salmonella enterica ser. typhimurium, vibrio cholerae and shigella dysenteriae in riverbed sediments of the apies river. experiments were performed in flow chambers containing three sediment types and con ... | 2016 | 27193001 |
| comparison of methods for quantitating salmonella enterica typhimurium and heidelberg strain attachment to reusable plastic shipping container coupons and preliminary assessment of sanitizer efficacy. | salmonella serovars, one of the leading contributors to foodborne illness and are especially problematic for foods that are not cooked before consumption, such as fresh produce. the shipping containers that are used to transport and store fresh produce may play a role in cross contamination and subsequent illnesses. however, methods for quantitatively attached cells are somewhat variable. the overall goal of this study was to compare conventional plating with molecular methods for quantitating a ... | 2016 | 27192211 |
| antimicrobial susceptibility of enteric gram negative facultative anaerobe bacilli in aerobic versus anaerobic conditions. | antimicrobial treatments result in the host's enteric bacteria being exposed to the antimicrobials. pharmacodynamic models can describe how this exposure affects the enteric bacteria and their antimicrobial resistance. the models utilize measurements of bacterial antimicrobial susceptibility traditionally obtained in vitro in aerobic conditions. however, in vivo enteric bacteria are exposed to antimicrobials in anaerobic conditions of the lower intestine. some of enteric bacteria of food animals ... | 2016 | 27191612 |
| strains, mechanism, and perspective: salmonella-based cancer therapy. | recently, investigation of bacterial-based tumor therapy has regained focus due to progress in molecular, cellular, and microbial biology. many bacteria such as salmonella, listeria, escherichia, and clostridium have proved to have tumor targeting and in some cases even tumor-destroying phenotypes. furthermore, bacterial clinical treatments for cancer have been improved by combination with other therapeutic methods such as chemotherapeutic drugs and radioactive agents. synthetic biology techniqu ... | 2016 | 27190519 |
| salmonella enterica subspecies diarizonae maxillary sinusitis in a snake handler: first report. | in this study, we report the first case of reptile-associated maxillary sinusitis due to salmonella enterica subspecies diarizonae in a snake handler and the third case of salmonella-associated sinusitis worldwide. the case highlights the potential of respiratory transmission and atypical salmonellosis presentations. | 2016 | 27186588 |
| clustered intracellular salmonella enterica serovar typhimurium blocks host cell cytokinesis. | several bacterial pathogens and viruses interfere with the cell cycle of their host cells to enhance virulence. this is especially apparent in bacteria that colonize the gut epithelium, where inhibition of the cell cycle of infected cells enhances the intestinal colonization. we found that intracellular salmonella enterica serovar typhimurium induced the binucleation of a large proportion of epithelial cells by 14 h postinvasion and that the effect was dependent on an intact salmonella pathogeni ... | 2016 | 27185791 |
| use of attenuated but metabolically competent salmonella as a probiotic to prevent or treat salmonella infection. | salmonella enterica is among the most burdensome of foodborne disease agents. there are over 2,600 serovars that cause a range of disease manifestations ranging from enterocolitis to typhoid fever. while there are two vaccines in use in humans to protect against typhoid fever, there are none that prevent enterocolitis. if vaccines preventing enterocolitis were to be developed, they would likely protect against only one or a few serovars. in this report, we tested the hypothesis that probiotic or ... | 2016 | 27185789 |
| immune response of turkey poults exposed at 1 day of age to either attenuated or wild salmonella strains. | salmonellosis is a foodborne zoonosis that is most often acquired by consuming poultry products such as eggs and poultry meat. amongst other measures the vaccination of food-producing poultry is thought to contribute to a reduction in human salmonellosis. in the european union (eu) in 2014 the licence of a commercially available salmonella vaccine for chickens and ducks was extended to turkeys. in the present study, we examined the course of infection with a virulent salmonella enterica ssp. ent ... | 2016 | 27185257 |
| evaluation of moringa oleifera seed lectin as a metal remover in aqueous solutions. | the efficacy of moringa oleifera seed lectin (wsmol) as a metal remover in water and the effects of metals on its hemagglutinating and antibacterial activities were determined. aqueous metal solutions were incubated with wsmol for 8 h at 4°c and the concentrations of metals were determined using atomic absorption spectroscopy. hemagglutination and antibacterial assays were conducted with wsmol and lectin exposed or not to the metals. the removal efficiency of wsmol was 49.00%, 53.21%, 71.45%, 55 ... | 2016 | 27184499 |
| recent advances in the characterization of crl, the unconventional activator of the stress sigma factor σs/rpos. | the bacterial rna polymerase (rnap) holoenzyme is a multisubunit core enzyme associated with a σ factor that is required for promoter-specific transcription initiation. besides a primary σ responsible for most of the gene expression during active growth, bacteria contain alternative σ factors that control adaptive responses. a recurring strategy in the control of σ factor activity is their sequestration by anti-sigma factors that occlude the rnap binding determinants, reducing their activity. in ... | 2016 | 27180360 |
| a prime-boost immunization with tc52 n-terminal domain dna and the recombinant protein expressed in pichia pastoris protects against trypanosoma cruzi infection. | we have previously reported that the n-terminal domain of the antigen tc52 (ntc52) is the section of the protein that confers the strongest protection against trypanosoma cruzi infection. to improve vaccine efficacy, we conducted here a prime-boost strategy (ntc52pb) by inoculating two doses of pcdna3.1 encoding the ntc52 dna carried by attenuated salmonella (sntc52), followed by two doses of recombinant ntc52 expressed in picchia pastoris plus odn-cpg as adjuvant. this strategy was comparativel ... | 2016 | 27177947 |
| a novel procedure on next generation sequencing data analysis using text mining algorithm. | next-generation sequencing (ngs) technologies have provided researchers with vast possibilities in various biological and biomedical research areas. efficient data mining strategies are in high demand for large scale comparative and evolutional studies to be performed on the large amounts of data derived from ngs projects. topic modeling is an active research field in machine learning and has been mainly used as an analytical tool to structure large textual corpora for data mining. | 2016 | 27177941 |
| whole-genome sequence of salmonella enterica serovar enteritidis phage type 4, isolated from a brazilian poultry farm. | the draft genome of salmonella enterica serovar enteritidis phage type 4 (pt4) strain ioc4647/2004, isolated from a poultry farm in são paulo state, was obtained with high-throughput illumina sequencing platform, generating 4,173,826 paired-end reads with 251 bp. the assembly of 4,804,382 bp in 27 scaffolds shows strong similarity to other s enteritidis strains. | 2016 | 27174265 |
| composing a tumor specific bacterial promoter. | systemically applied salmonella enterica spp. have been shown to invade and colonize neoplastic tissues where it retards the growth of many tumors. this offers the possibility to use the bacteria as a vehicle for the tumor specific delivery of therapeutic molecules. specificity of such delivery is solely depending on promoter sequences that control the production of a target molecule. we have established the functional structure of bacterial promoters that are transcriptionally active exclusivel ... | 2016 | 27171245 |
| house sparrows do not constitute a significant salmonella typhimurium reservoir across urban gradients in flanders, belgium. | in recent decades major declines in urban house sparrow (passer domesticus) populations have been observed in north-western european cities, whereas suburban and rural house sparrow populations have remained relatively stable or are recovering from previous declines. differential exposure to avian pathogens known to cause epidemics in house sparrows may in part explain this spatial pattern of declines. here we investigate the potential effect of urbanization on the development of a bacterial pat ... | 2016 | 27168186 |
| self-association motifs in the enteroaggregative escherichia coli heat-resistant agglutinin 1. | the heat-resistant agglutinin 1 (hra1) is an integral outer membrane protein found in strains of escherichia coli that are exceptional colonizers. hra1 from enteroaggregative e. coli strain 042 is sufficient to confer adherence to human epithelial cells and to cause bacterial autoaggregation. hra1 is closely related to the tia invasin, which also confers adherence, but not autoaggregation. here, we have demonstrated that hra1 mediates autoaggregation by self-association and we hypothesize that a ... | 2016 | 27166217 |
| curing vector for inci1 plasmids and its use to provide evidence for a metabolic burden of inci1 ctx-m-1 plasmid pifm3791 on klebsiella pneumoniae. | using a sequence-based approach we previously identified an inci1 ctx-m-1 plasmid, pifm3791, on a single pig farm in the uk that was harboured by klebsiella pneumoniae, escherichia coli and salmonella enterica serotype 4,5,12:i:-. to test the hypothesis that the plasmid had spread rapidly into these differing host bacteria we wished to assess whether the plasmid conferred a fitness advantage. to do this an inci1 curing vector was constructed and used to displace the inci1 ctx-m-1 plasmids from k ... | 2016 | 27166141 |
| molecular analysis of fluoroquinolone resistance in salmonella enterica serovar typhi from a breast abscess case. | 2017 | 27166067 | |
| pediatric epidemic of salmonella enterica serovar typhimurium in the area of l'aquila, italy, four years after a catastrophic earthquake. | a salmonella enterica epidemic occurred in children of the area of l'aquila (central italy, abruzzo region) between june 2013 and october 2014, four years after the catastrophic earthquake of 6 april 2009. | 2016 | 27164121 |
| construction and characterization of a cigr deletion mutant of salmonella enterica serovar pullorum. | salmonella enterica serovar pullorum (s. pullorum) is the causative agent of pullorum disease (pd) and results in severe economic losses to the poultry industry. as a salmonella type iii secretion system 2 (t3ss2) effector and predicted membrane protein, cigr is encoded by the cigr gene within salmonella pathogenicity island 3 (spi3). in order to research the influence of the cigr gene on s. pullorum, a cigr mutant of s. pullorum s06004 was constructed by the lambda red recombination system, and ... | 2016 | 27163262 |
| probiotic properties of lactic acid bacteria isolated from human milk. | the objective of this study was to identify and characterize lactic acid bacteria isolated from human milk, with an emphasis on their probiotic properties. | 2016 | 27159339 |
| rapid dna transformation in salmonella typhimurium by the hydrogel exposure method. | even with advances in molecular cloning and dna transformation, new or alternative methods that permit dna penetration in salmonella enterica subspecies enterica serovar typhimurium are required in order to use this pathogen in biotechnological or medical applications. in this work, an adapted protocol of bacterial transformation with plasmid dna based on the "yoshida effect" was applied and optimized on salmonella enterica serovar typhimurium lt2 reference strain. the plasmid transference based ... | 2016 | 27154729 |
| non-typhoidal salmonella colonization in chickens and humans in the mekong delta of vietnam. | salmonellosis is a public health concern in both the developed and developing countries. although the majority of human non-typhoidal salmonella enterica (nts) cases are the result of foodborne infections or person-to-person transmission, nts infections may also be acquired by environmental and occupational exposure to animals. while a considerable number of studies have investigated the presence of nts in farm animals and meat/carcasses, very few studies have investigated the risk of nts coloni ... | 2017 | 27152998 |
| genome sequences of salmonella enterica subsp. enterica serovar lubbock strains isolated from liver abscesses of feedlot cattle. | the genome sequencing of 13 salmonella enterica subsp. enterica serovar lubbock strains isolated from liver abscesses of feedlot cattle is reported here. the availability of these genomes will help to further understand the etiologic role of salmonella strains in liver abscesses of cattle and will serve as references in microbial trace-back studies to improve food safety. | 2016 | 27151794 |
| draft genome sequences of 37 salmonella enterica strains isolated from poultry sources in nigeria. | here, we report the availability of draft genomes of several salmonella serotypes, isolated from poultry sources from nigeria. these genomes will help to further understand the biological diversity of s. enterica and will serve as references in microbial trace-back studies to improve food safety. | 2016 | 27151793 |
| isolation of lactic acid bacteria from swine milk and characterization of potential probiotic strains with antagonistic effects against swine-associated gastrointestinal pathogens. | probiotics are usually isolated from the gastrointestinal tract of humans and animals. the search of probiotics in human milk is a recent field of research, as the existence of the human milk microbiome was discovered only about a decade ago. to our knowledge, no reports regarding the potential probiotic effect of bacteria from swine milk have been published. in this work, we isolated several lactic acid bacteria from swine milk and evaluated them for them potential as probiotics. among the isol ... | 2016 | 27149540 |
| a multischool outbreak due to salmonella enterica serovar napoli associated with elevated rates of hospitalizations and bacteremia, milan, italy, 2014. | a multischool outbreak of salmonellosis caused by salmonella enterica serovar napoli was investigated in the province of milan from october to november 2014, following an increase in school absenteeism coinciding with two positive cases. epidemiological studies detected 47 cases in four primary schools: 46 children and 1 adult woman (51.4% males and 48.6% females, median age 8.9). from these, 14 cases (29.8%) were severe and resulted in hospitalization, including 6 children (12.8%) who developed ... | 2016 | 27148636 |
| chitin mixed in potting soil alters lettuce growth, the survival of zoonotic bacteria on the leaves and associated rhizosphere microbiology. | chitin is a promising soil amendment for improving soil quality, plant growth, and plant resilience. the objectives of this study were twofold. first, to study the effect of chitin mixed in potting soil on lettuce growth and on the survival of two zoonotic bacterial pathogens, escherichia coli o157:h7 and salmonella enterica on the lettuce leaves. second, to assess the related changes in the microbial lettuce rhizosphere, using phospholipid fatty acid (plfa) analysis and amplicon sequencing of a ... | 2016 | 27148242 |
| biosynthesis of silver nanoparticles by novosphingobium sp. thg-c3 and their antimicrobial potential. | the present study described biosynthesis of silver nanoparticles (agnps) using a bacterial strain novosphingobium sp. thg-c3, isolated from soil, and their application in antibacterial activity. the maximum absorbance values of the synthesized agnps was measured at 406 nm in ultraviolet-visible spectrophotometry and were mostly spherical in shape with particle size in range of 8-25 nm by field emission transmission electron microscopy analysis. x-ray diffraction pattern corresponding to planes ( ... | 2017 | 27145847 |
| antimicrobial resistance and class 1 integron-associated gene cassettes in salmonella enterica serovar typhimurium isolated from pigs at slaughter and abattoir environment. | forty-five multi-resistant salmonella enterica subsp. enterica serovar (s.) typhimurium isolates obtained at five pig abattoirs in southern brazil were characterized. their relatedness was determined by xbai-macrorestriction analysis. resistance genes, integrons and plasmid-mediated quinolone resistance genes (pmqr) were investigated by pcr. amplicons for the variable part of class 1 integrons and the quinolone resistance-determining regions (qrdr) were sequenced. plasmids were characterized by ... | 2016 | 27142182 |
| the salmonella pathogenicity island 13 contributes to pathogenesis in streptomycin pre-treated mice but not in day-old chickens. | salmonella enterica serovar enteritidis (s. enteritidis) is a human and animal pathogen that causes gastroenteritis characterized by inflammatory diarrhea and occasionally an invasive systemic infection. salmonella pathogenicity islands (spis) are horizontally acquired genomic segments known to contribute to salmonella pathogenesis. the objective of the current study was to determine the contribution of spi-13 to s. enteritidis pathogenesis. | 2016 | 27141235 |
| preventing invasive salmonellosis in children through vaccination. | vaccination is an important strategy to control endemic enteric fever (ef) and to interrupt transmission during outbreaks. the main aim of this paper is to discuss the efficacy of available ef vaccines in children and to highlight novel vaccination possibilities against ef and non-typhoid invasive salmonelloses. | 2016 | 27140662 |
| preparation and biological activity of new collagen composites, part i: collagen/zinc titanate nanocomposites. | the aim of this investigation was to develop new antimicrobial collagen/zinc titanate (zntio3) biomaterials using a sol-gel cryogenic draying technology in keeping the native collagen activity. broad-spectrum antimicrobial activity was demonstrated against firmicutes (staphylococcus epidermidis, bacillus cereus, and candida lusitaniae) and gracilicutes (escherichia coli, salmonella enterica, and pseudomonas putida) microorganisms. the antimicrobial activity as well as the cytotoxicity were speci ... | 2016 | 27138724 |
| altered virulence potential of salmonella enteritidis cultured in different foods: a cumulative effect of differential gene expression and immunomodulation. | salmonella enterica serovars enteritidis (s. enteritidis) is one of the most common causes of food borne illness. bacterial growth environment plays an important role in regulating gene expression thereby affecting the virulence profile of the bacteria. different foods present diverse growth conditions which may affect the pathogenic potential of the bacteria. in the present study, the effect of food environments on the pathogenic potential of s. enteritidis has been evaluated. s. enteritidis wa ... | 2016 | 27132148 |
| salmonella pneumonia complicated with encysted empyema in an immunocompromised youth: case report and literature review. | in this case report we described a bahraini male patient of twenty years of age, a smoker and diagnosed with stage iv b hodgkin lymphoma. he presented with fever, nonproductive cough, upper back pain and shortness of breath due to right upper lobe pneumonia with right encysted pleural effusion. salmonella enterica serotype enteritidis was isolated from the sputum. he was successfully treated with 2 weeks of ceftriaxone followed by 2 weeks of oral cefixime. this was the first case of encysted emp ... | 2016 | 27131011 |
| carriage prevalence of salmonella enterica serotype typhi in gallbladders of adult autopsy cases from mozambique. | typhoid fever is an important public health problem in many low-income countries where asymptomatic carriers play an important role in its dissemination. the bacterium causing typhoid fever can live in the gallstones of asymptomatic persons after the infection. these carriers are reservoirs of s. typhi, are highly contagious, and spread the disease through the secretion of bacteria in feces and urine. the aim of this study was to determine the carrier rate in an area of mozambique. | 2016 | 27131005 |
| development of an avirulent salmonella surrogate for modeling pathogen behavior in pre- and postharvest environments. | recurrent outbreaks of bacterial gastroenteritis linked to the consumption of fresh fruits and vegetables highlight the paucity of understanding of the ecology of salmonella enterica under crop production and postharvest conditions. these gaps in knowledge are due, at least in part, to the lack of suitable surrogate organisms for studies for which biosafety level 2 is problematic. therefore, we constructed and validated an avirulent strain of salmonella enterica serovar typhimurium. the strain l ... | 2016 | 27129962 |
| what's on the outside matters: the role of the extracellular polymeric substance of gram-negative biofilms in evading host immunity and as a target for therapeutic intervention. | biofilms are organized multicellular communities encased in an extracellular polymeric substance (eps). biofilm-resident bacteria resist immunity and antimicrobials. the eps provides structural stability and presents a barrier; however, a complete understanding of how eps structure relates to biological function is lacking. this review focuses on the eps of three gram-negative pathogens: pseudomonas aeruginosa, nontypeable haemophilus influenzae, and salmonella enterica serovar typhi/typhimurium ... | 2016 | 27129225 |
| emergence of host-adapted salmonella enteritidis through rapid evolution in an immunocompromised host. | host adaptation is a key factor contributing to the emergence of new bacterial, viral and parasitic pathogens. many pathogens are considered promiscuous because they cause disease across a range of host species, while others are host-adapted, infecting particular hosts(1). host adaptation can potentially progress to host restriction where the pathogen is strictly limited to a single host species and is frequently associated with more severe symptoms. host-adapted and host-restricted bacterial cl ... | 2016 | 27127642 |
| bacteriophages with potential to inactivate salmonella typhimurium: use of single phage suspensions and phage cocktails. | the aim of this study was to compare the dynamics of three previously isolated bacteriophages (or phages) individually (phse-1, phse-2 and phse-5) or combined in cocktails of two or three phages (phse-1/phse-2, phse-1/phse-5, phse-2/phse-5 and phse-1/phse-2/phse-5) to control salmonella enterica serovar typhimurium (salmonella typhimurium) in order to evaluate their potential application during depuration. phages were assigned to the family siphoviridae and revealed identical restriction digest ... | 2016 | 27126773 |
| pharmacokinetic and pharmacodynamic evaluations of a 10 mg/kg enrofloxacin intramuscular administration in bearded dragons (pogona vitticeps): a preliminary assessment. | enrofloxacin (e) is commonly used in veterinary medicine. it is necessary to perform pharmacokinetic/dynamic studies to minimize the selection of resistant mutants of bacteria and extend the efficacy of antimicrobial agents. eight healthy adult pogona vitticeps were assigned into two groups of equal size and treated with a single intramuscular injection of e at 10 mg/kg. blood samples were withdrawn at different scheduled times for each group, and rectal swabs were collected. e and ciprofloxacin ... | 2017 | 27121980 |
| photoinactivation of eight health-relevant bacterial species: determining the importance of the exogenous indirect mechanism. | it is presently unknown to what extent the endogenous direct, endogenous indirect, and exogenous indirect mechanisms contribute to bacterial photoinactivation in natural surface waters. in this study, we investigated the importance of the exogenous indirect mechanism by conducting photoinactivation experiments with eight health-relevant bacterial species (bacteroides thetaiotaomicron, campylobacter jejuni, enterococcus faecalis, escherichia coli k12, e. coli o157:h7, salmonella enterica serovar ... | 2016 | 27121126 |
| dynamic duo-the salmonella cytolethal distending toxin combines adp-ribosyltransferase and nuclease activities in a novel form of the cytolethal distending toxin. | the cytolethal distending toxin (cdt) is a well characterized bacterial genotoxin encoded by several gram-negative bacteria, including salmonella enterica (s. enterica). the cdt produced by salmonella (s-cdt) differs from the cdt produced by other bacteria, as it utilizes subunits with homology to the pertussis and subtilase toxins, in place of the traditional cdta and cdtc subunits. previously, s-cdt was thought to be a unique virulence factor of s. enterica subspecies enterica serotype typhi, ... | 2016 | 27120620 |
| emergence of blactx-m-15, blatem-169 and blaper-1 extended-spectrum β-lactamase genes among different salmonella enterica serovars from human faecal samples. | broad-spectrum β-lactams are used for empirical therapy of severe infections with non-typhoid salmonella serotypes; however, activities of these drugs against the strains producing different β-lactamase is not so clear. this study investigated the prevalence of β-lactamase genes among isolates of s. enterica serovars from human faecal samples and determined their diversity in activity against different β-lactams. | 2016 | 27117981 |
| analysis of the fluoroquinolone antibiotic resistance mechanism of salmonella enterica isolates. | quinolone-resistant salmonella strains were isolated from patient samples, and several quinolone-sensitive strains were used to analyze mutations in the quinolone resistance-determining region (qrdr) of gyra, gyrb, parc, and pare and to screen for plasmid-mediated quinolone resistance. among the 21 strains that showed resistance to nalidixic acid and ciprofloxacin (mic 0.125-2.0 μg/ml), 17 strains had a mutation in qrdr codon 87 of gyra, and 3 strains had a single mutation (ser83 → phe). another ... | 2016 | 27116992 |
| geographical and temporal variation of e. coli and norovirus in mussels. | bivalve shellfish may accumulate contaminants of public health concern including pathogenic bacteria and viruses. microbiological monitoring of production areas is based on faecal coliforms in water in the usa and escherichia coli in bivalve molluscs in the european union. e. coli is known to reflect contamination with salmonella enterica but not necessarily with other human pathogens such as enteric viruses. a structured field study was undertaken at three locations in order to investigate the ... | 2016 | 27114089 |
| the in vitro redundant enzymes purn and purt are both essential for systemic infection of mice in salmonella enterica serovar typhimurium. | metabolic enzymes show a high degree of redundancy, and for that reason they are generally ignored in searches for novel targets for anti-infective substances. the enzymes purn and purt are redundant in vitro in salmonella enterica serovar typhimurium, in which they perform the third step of purine synthesis. surprisingly, the results of the current study demonstrated that single-gene deletions of each of the genes encoding these enzymes caused attenuation (competitive infection indexes [ci] of ... | 2016 | 27113361 |
| a case study on salmonella enterica serovar typhimurium at a dairy farm associated with massive sparrow death. | salmonella enterica typhimurium (s. typhimurium) is the most common cause of bovine salmonellosis in japan and where it is also cause of salmonellosis in wild birds. in 2008, a postpartum cow at a dairy farm developed diarrhea caused by s. typhimurium. the herd was extensively surveilled for salmonella sp. and we characterized bacterial isolates from this and other cows to determine the source of infection. | 2016 | 27112780 |
| potential antimicrobial and antiproliferative activities of autochthonous starter cultures and protease epg222 in dry-fermented sausages. | this work studied the presence of nitrogen compounds with bioactive properties in iberian pork sausages that were manufactured using different autochthonous starter cultures (pediococcus acidilactici ms200 and staphylococcus vitulus rs34) and protease epg222. nitrogen compounds were extracted and evaluated for their antimicrobial effect against spoilage and pathogenic bacteria, such as bacillus cereus, escherichia coli, salmonella choleraesuis, staphylococcus aureus and listeria monocytogenes, a ... | 2016 | 27112426 |
| nosocomial outbreak of a novel extended-spectrum β-lactamase salmonella enterica serotype isangi among surgical patients. | objective nosocomial outbreaks caused by salmonella are rare. we describe the investigation and control of a cluster of novel extended-spectrum β-lactamase (esbl) salmonella enterica serotype isangi in a hospital in southeastern michigan. methods an epidemiologic investigation, including case-control study, assessment of infection control practices and environmental cultures, was performed to identify modes of transmission. healthcare workers (hcws) exposed to case patients were screened. strain ... | 2016 | 27108715 |
| outbreak of unusual salmonella enterica serovar typhimurium monophasic variant 1,4 [5],12:i:-, italy, june 2013 to september 2014. | monophasic variant of salmonella enterica subspecies enterica serovar typhimurium (monophasic s. typhimurium), with antigenic structure 1,4,[5],12:i:-, appears to be of increasing importance in europe. in italy, monophasic s. typhimurium represented the third most frequent salmonella serovar isolated from human cases between 2004 and 2008. from june 2013 to october 2014, a total of 206 human cases of salmonellosis were identified in abruzzo region (central italy). obtained clinical isolates char ... | 2016 | 27105170 |
| assessing the effect of sodium dichloroisocyanurate concentration on transfer of salmonella enterica serotype typhimurium in wash water for production of minimally processed iceberg lettuce (lactuca sativa l.). | this study evaluated the impact of sodium dichloroisocyanurate (5, 10, 20, 30, 40, 50 and 250 mg l(-1) ) in wash water on transfer of salmonella typhimurium from contaminated lettuce to wash water and then to other noncontaminated lettuces washed sequentially in the same water. experiments were designed mimicking the conditions commonly seen in minimally processed vegetable (mpv) processing plants in brazil. the scenarios were as follows: (1) washing one inoculated lettuce portion in nonchlorina ... | 2016 | 27105015 |
| mp-v1 from the venom of social wasp vespula vulgaris is a de novo type of mastoparan that displays superior antimicrobial activities. | mastoparans from the venom of social wasps have attracted considerable attention as effective antibiotic candidates. in this study, mastoparan v1 (mp-v1) from vespula vulgaris was first disclosed to have a peptide amino acid sequence distinct from typical mastoparans and its biochemical properties and antimicrobial effects were compared with those of typical mastoparans mp-l, -x(v) and -b. circular dichroism (cd) spectroscopy revealed that mp-v1 and -x(v) form more stable α-helical conformations ... | 2016 | 27104500 |
| complete genome sequence of salmonella enterica serovar typhimurium strain so3 (sequence type 302) isolated from a baby with meningitis in mexico. | the complete genome of italic! salmonella entericaserovar typhimurium strain so3 (sequence type 302), isolated from a fatal meningitis infection in mexico, was determined using pacbio technology. the chromosome hosts six complete prophages and is predicted to harbor 51 genomic islands, including 13 pathogenicity islands (spis). it carries the italic! salmonellavirulence plasmid (pstv). | 2016 | 27103717 |
| draft genome sequences of salmonella enterica subsp. enterica serovar berta atcc 8392 and a nalidixic acid-resistant isolate of this strain. | italic! salmonella entericasubspecies italic! entericaserovar berta has been isolated in multiple animal species and has been implicated in human disease. here, we report a 4.7-mbp draft genome sequence of italic! s. entericaserovar berta (atcc strain 8392) and a nalidixic acid-resistant isolate derived from this strain. | 2016 | 27103707 |
| complete nucleotide sequence of ph11, an inchi2 plasmid conferring multi-antibiotic resistance and multi-heavy metal resistance genes in a clinical klebsiella pneumoniae isolate. | the complete 284,628bp sequence of ph11, an inchi2 plasmid, was determined through single-molecule, real-time (smrt) sequencing. harbored by a clinical klebsiella pneumoniae strain h11, and isolated in beijing, this plasmid contains multiple antibiotic resistance genes, including cata2, aac(6')-ib, strb, stra, dfra19, blatem-1, blashv-12, sul1, qace delta 1, erea, arr2, and aac3. the aac(6')-ib is carried by a class i integron. plasmid ph11 also carries several genes associated with resistance t ... | 2016 | 27101788 |
| biocontrol and rapid detection of food-borne pathogens using bacteriophages and endolysins. | bacteriophages have been suggested as natural food preservatives as well as rapid detection materials for food-borne pathogens in various foods. since listeria monocytogenes-targeting phage cocktail (listshield) was approved for applications in foods, numerous phages have been screened and experimentally characterized for phage applications in foods. a single phage and phage cocktail treatments to various foods contaminated with food-borne pathogens including e. coli o157:h7, salmonella enterica ... | 2016 | 27092128 |
| curcumin reduces the motility of salmonella enterica serovar typhimurium by binding to the flagella, thereby leading to flagellar fragility and shedding. | one of the important virulence properties of the pathogen is its ability to travel to a favorable environment, cross the viscous mucus barrier (intestinal barrier for enteric pathogens), and reach the epithelia to initiate pathogenesis with the help of an appendage, like flagella. nonetheless, flagella can act as an "achilles heel," revealing the pathogen's presence to the host through the stimulation of innate and adaptive immune responses. we assessed whether curcumin, a dietary polyphenol, co ... | 2016 | 27091154 |
| detection of the plasmid-mediated mcr-1 gene conferring colistin resistance in human and food isolates of salmonella enterica and escherichia coli in england and wales. | in response to the first report of transmissible colistin resistance mediated by the mcr-1 gene in escherichia coli and klebsiella spp. from animals and humans in china, we sought to determine its presence in enterobacteriaceae isolated in the uk. | 2016 | 27090630 |
| generation of a naïve human single chain variable fragment (scfv) library for the identification of monoclonal scfv against salmonella typhi hemolysin e antigen. | antibody phage display is a useful tool for the isolation and identification of monoclonal antibodies. naive antibody libraries are able to overcome the limitations associated with the traditional hybridoma method for monoclonal antibody generation. antibody phage display is also a preferred method for antibody generation against toxins as it does not suffer from toxicity mediated complications. here, we describe a naïve multi ethnic scfv antibody library generated via two-step cloning with an e ... | 2016 | 27090555 |
| genome sequence of the fish pathogen yersinia ruckeri sc09 provides insights into niche adaptation and pathogenic mechanism. | yersinia ruckeri is the etiologic agent of enteric red mouth disease (erm), a severe fish disease prevailing in worldwide aquaculture industries. here we report for the first time the complete genome of y. ruckeri (yersinia ruckeri) sc09, a highly virulent strain isolated from ictalurus punctatus with severe septicemia. sc09 possesses a single chromosome of 3,923,491 base pairs, which contains 3651 predicted protein coding sequences (cds), 19 rrna genes, and 79 trna genes. among the cds, we have ... | 2016 | 27089334 |
| serotypes and antimicrobial resistance of salmonella enterica isolated from pork, chicken meat and lettuce, bangkok and central thailand. | food of animal origins, particularly pork and chicken meat, has long been recognized as major sources of human salmonellosis. there have been recent reports of human salmonellosis outbreaks due to consumption of leafy green vegetables such as lettuce. in this study, 120 (40 pork, 40 chicken meat and 40 lettuce) samples were randomly collected from retail markets in bangkok and central thailand during june to august 2015 for salmonella serotype identification and antimicrobial susceptibility test ... | 2016 | 27086423 |
| effect of dss on bacterial growth in gastrointestinal tract. | inflammatory bowel disease is an idiopathic autoimmune disorder that is mainly divided into ulcerative colitis and crohn's disease. probiotics are known for their beneficial effect and used as a treatment option in different gastrointestinal problems. the aim of our study was to find suitable bacterial vectors for gene therapy of inflammatory bowel disease. salmonella enterica serovar typhimurium sl7207 and escherichia coli nissle 1917 were investigated as potential vectors. our results show tha ... | 2016 | 27085009 |
| status of paratyphoid fever vaccine research and development. | salmonella enterica serovars typhi and paratyphi (s. paratyphi) a and b cause enteric fever in humans. of the paratyphoid group, s. paratyphi a is the most common serovar. in 2000, there were an estimated 5.4 million cases of s. paratyphi a worldwide. more recently paratyphoid fever has accounted for an increasing fraction of all cases of enteric fever. although vaccines for typhoid fever have been developed and in use for decades, vaccines for paratyphoid fever have not yet been licensed. sever ... | 2016 | 27083427 |
| transmissible plasmid containing salmonella enterica heidelberg isolates modulate cytokine production during early stage of interaction with intestinal epithelial cells. | the variation in cytokine production during bacterial invasion of human intestinal epithelial cells (iecs) is a contributing factor for progression of the infection. a few salmonella enterica heidelberg strains isolated from poultry products harbor transmissible plasmids (tps), including those that encode a type-iv secretion system. earlier, we showed that these tps are responsible for increased virulence during infection. this study examines the potential role of these tps in cytokine productio ... | 2016 | 27082282 |
| influence of 6 different intestinal bacteria on beltsville small white turkey semen. | the turkey industry relies totally on artificial insemination to continue and improve production. if something compromises the insemination process, such as contaminated semen, it becomes a detrimental loss to the industry. bacteria have been found in broiler breeder males to reduce sperm motility. the sperm quality index (sqi) is a quick method to determine avian sperm motility using the sperm quality analyzer (sqa). therefore, the objective of this study was to determine if bacteria have an ef ... | 2016 | 27081196 |
| complete genome sequence of salmonella enterica serovar typhimurium strain so2 (sequence type 302) isolated from an asymptomatic child in mexico. | the complete genome sequence ofsalmonella entericaserovar typhimurium strain so2, isolated from an asymptomatic child in mexico, was determined using pacbio single-molecule real-time technology. strain so2 has six complete chromosomal prophages, namely, st104, gifsy-2, st64b, gifsy-1, elphis, and fsl sp-004, and carries asalmonellavirulence plasmid. | 2016 | 27081133 |
| complete genome sequence of salmonella enterica serovar typhimurium strain yu15 (sequence type 19) harboring the salmonella genomic island 1 and virulence plasmid pstv. | the complete genome ofsalmonella entericasubsp.entericaserovar typhimurium sequence type 19 (st19) strain yu15, isolated in yucatán, mexico, from a human baby stool culture, was determined using pacbio technology. the chromosome contains five intact prophages and thesalmonellagenomic island 1 (sgi1). this strain carries thesalmonellavirulence plasmid pstv. | 2016 | 27081132 |
| depletion of butyrate-producing clostridia from the gut microbiota drives an aerobic luminal expansion of salmonella. | the mammalian intestine is host to a microbial community that prevents pathogen expansion through unknown mechanisms, while antibiotic treatment can increase susceptibility to enteric pathogens. here we show that streptomycin treatment depleted commensal, butyrate-producing clostridia from the mouse intestinal lumen, leading to decreased butyrate levels, increased epithelial oxygenation, and aerobic expansion of salmonella enterica serovar typhimurium. epithelial hypoxia and salmonella restricti ... | 2016 | 27078066 |
| the type three secreted effector sipc regulates the trafficking of perp during salmonella infection. | salmonella enterica typhimurium employs type iii secreted effectors to induce cellular invasion and pathogenesis. we previously reported the secreted effector sipa is in part responsible for inducing the apical accumulation of the host membrane protein perp, a host factor we have shown is key to the inflammatory response induced by salmonella. we now report that the s. typhimurium type iii secreted effector sipc significantly contributes to perp redistribution to the apical membrane surface. to ... | 2016 | 27078059 |
| lipopolysaccharide modification in gram-negative bacteria during chronic infection. | the gram-negative bacterial lipopolysaccharide (lps) is a major component of the outer membrane that plays a key role in host-pathogen interactions with the innate immune system. during infection, bacteria are exposed to a host environment that is typically dominated by inflammatory cells and soluble factors, including antibiotics, which provide cues about regulation of gene expression. bacterial adaptive changes including modulation of lps synthesis and structure are a conserved theme in infect ... | 2016 | 27075488 |
| salmonella infection and carriage in reptiles in a zoological collection. | objective to identify important subspecies and serovars of salmonella enterica in a captive reptile population and clinically relevant risk factors for and signs of illness in salmonella-positive reptiles. design retrospective cross-sectional study. animals 11 crocodilians (4 samples), 78 snakes (91 samples), 59 lizards (57 samples), and 34 chelonians (23 samples) at the bronx zoo from 2000 through 2012. procedures data pertaining to various types of biological samples obtained from reptiles wit ... | 2016 | 27074614 |
| transfer rna bound to mnmh protein is enriched with geranylated trna--a possible intermediate in its selenation? | the wobble nucleoside 5-methylaminomethyl-2-thio-uridine (mnm5s2u) is present in bacterial trnas specific for lys and glu and 5-carboxymethylaminomethyl-2-thio-uridine (cmnm5s2u) in trna specific for gln. the sulfur of (c)mnm5s2u may be exchanged by selenium (se)-a reaction catalyzed by the selenophosphate-dependent trna 2-selenouridine synthase encoded by the mnmh (ybbb, selu, sufy) gene. the mnmh protein has a rhodanese domain containing one catalytic cys (c97) and a p-loop domain containing a ... | 2016 | 27073879 |
| mapping polyclonal antibody responses to bacterial infection using next generation phage display. | mapping polyclonal antibody responses to infectious diseases to identify individual epitopes has the potential to underpin the development of novel serological assays and vaccines. here, phage-peptide library panning coupled with screening using next generation sequencing was used to map antibody responses to bacterial infections. in the first instance, pigs experimentally infected with salmonella enterica serovar typhimurium was investigated. igg samples from twelve infected pigs were probed in ... | 2016 | 27072017 |
| selection and characterization of aptamers using a modified whole cell bacterium selex for the detection of salmonella enterica serovar typhimurium. | this study describes the selection of single-stranded dna (ssdna) aptamers against salmonella enterica serovar typhimurium using a modified whole cell systematic evolution of ligands by exponential enrichment (whole cell selex). for evolving specific aptamers, ten rounds of selection to live salmonella cells, alternating with negative selection against a cocktail of related pathogens, were performed. the resulting highly enriched oligonucleotide pools were sequenced and clustered into eight grou ... | 2016 | 27070414 |
| multiple plasmids contribute to antibiotic resistance and macrophage survival in vitro in cmy2-bearing salmonella enterica. | multiple drug resistance (mdr) in bacteria represents a notable problem but if carried on plasmid their spread could become a significant threat to public health. plasmids in members of the enterobacteriaceae family and in particular salmonella and escherichia coli strains have been implicated in the spread of antibiotic resistance genes. however, the mechanisms involved in the transfer of plasmid-borne resistance genes are not fully understood. here, we analyzed the ability of salmonella enteri ... | 2016 | 27070176 |
| new role for fda-approved drugs in combating antibiotic-resistant bacteria. | antibiotic resistance in medically relevant bacterial pathogens, coupled with a paucity of novel antimicrobial discoveries, represents a pressing global crisis. traditional drug discovery is an inefficient and costly process; however, systematic screening of food and drug administration (fda)-approved therapeutics for other indications in humans offers a rapid alternative approach. in this study, we screened a library of 780 fda-approved drugs to identify molecules that rendered raw 264.7 murine ... | 2016 | 27067323 |
| dksa-dependent transcriptional regulation in salmonella experiencing nitrosative stress. | redox-based signaling is fundamental to the capacity of bacteria to sense, and respond to, nitrosative and oxidative stress encountered in natural and host environments. the conserved rna polymerase regulatory protein dksa is a thiol-based sensor of reactive nitrogen and oxygen species. dksa-dependent transcriptional control promotes antinitrosative and antioxidative defenses that contribute to salmonella pathogenesis. the specific adaptive changes mediated by dksa in response to reactive specie ... | 2016 | 27065993 |
| ctx-m-27 producing salmonella enterica serotypes typhimurium and indiana are prevalent among food-producing animals in china. | salmonella spp. is one of the most important food-borne pathogens causing digestive tract and invasive infections in both humans and animals. extended-spectrum β-lactamases (esbls) especially the ctx-m-type esbls are increasingly being reported worldwide and in china. these studies seldom focused on salmonella isolates from food-producing animals. the aim of this study was to characterize the antimicrobial resistance profiles, serotypes and esbls and in particular, ctx-m producing salmonella iso ... | 2016 | 27065989 |
| impacts of salmonella enterica serovar typhimurium and its speg gene on the transcriptomes of in vitro m cells and caco-2 cells. | microfold or membranous (m) cells are specialized intestinal epithelial cells responsible for host immunity. the speg mutant of salmonella typhimurium (s. typhimurium) is a nonreplicating strain within human cells to be a candidate vaccine vector for interacting with m cells. we conducted this study to identify the genes are differently expressed between in vitro m cells and caco-2 cells, and to determine whether s. typhimurium and speg affect the transcriptomes of both cell types. in vitro m ce ... | 2016 | 27064787 |
| engineering formation of multiple recombinant eut protein nanocompartments in e. coli. | compartmentalization of designed metabolic pathways within protein based nanocompartments has the potential to increase reaction efficiency in multi-step biosynthetic reactions. we previously demonstrated proof-of-concept of this aim by targeting a functional enzyme to single cellular protein nanocompartments, which were formed upon recombinant expression of the salmonella enterica lt2 ethanolamine utilization bacterial microcompartment shell proteins euts or eutsmnlk in escherichia coli. to opt ... | 2016 | 27063436 |
| salmonella flagellin acted as an effective fusion partner for expression of plasmodium falciparum surface protein 25 in escherichia coli. | plasmodium falciparum surface protein 25 (pfs25) is a hard-to-express and hard-to-solubilize protein in escherichia coli. to overcome this problem, the phase 1 flagellin of salmonella enterica serovar typhimurium (flic) was used as a fusion partner for pfs25. the fusion expression of pfs25 with flic greatly enhanced the expression level and solubility of pfs25 in e. coli bl21(de3). the ni-purified fusion protein of flic-pfs25 was recognized by two anti-pfs25 monoclonal antibodies. by comparison, ... | 2016 | 27058511 |
| biochemical and biophysical combined study of bicarinalin, an ant venom antimicrobial peptide. | we have recently characterized bicarinalin as the most abundant peptide from the venom of the ant tetramorium bicarinatum. this antimicrobial peptide is active against staphylococcus and enterobacteriaceae. to further investigate the antimicrobial properties of this cationic and cysteine-free peptide, we have studied its antibacterial, antifungal and antiparasitic activities on a large array of microorganisms. bicarinalin was active against fifteen microorganisms with minimal inhibitory concentr ... | 2016 | 27058430 |
| expression and secretion pattern of fljb of salmonella enterica serovar typhi in different culture conditions. | 2016 | 27057676 | |
| the typhoid toxin promotes host survival and the establishment of a persistent asymptomatic infection. | bacterial genotoxins, produced by several gram-negative bacteria, induce dna damage in the target cells. while the responses induced in the host cells have been extensively studied in vitro, the role of these effectors during the course of infection remains poorly characterized. to address this issue, we assessed the effects of the salmonella enterica genotoxin, known as typhoid toxin, in in vivo models of murine infection. immunocompetent mice were infected with isogenic s. enterica, serovar ty ... | 2016 | 27055274 |
| fluorescence-based thermal shift data on multidrug regulator acrr from salmonella enterica subsp. entrica serovar typhimurium str. lt2. | the fluorescence-based thermal shift (fts) data presented here include table s1 and fig. s1, and are supplemental to our original research article describing detailed structural, fts, and fluorescence polarization analyses of the salmonella enterica subsp. entrica serovar typhimurium str. lt2 multidrug transcriptional regulator acrr (stacrr) (doi:10.1016/j.jsb.2016.01.008) (manjasetty et al., 2015 [1]). table s1 contains chemical formulas, a chemical abstracts service (cas) registry number (cas ... | 2016 | 27054155 |