Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| evaluation of the immunocardstat! rotavirus assay for detection of group a rotavirus in fecal specimens. | rapid detection of group a rotavirus was performed by using immunocardstat! rotavirus (ics-rv) (which uses immunogold-based, horizontal-flow membrane technology), two commercial enzyme immunoassays (premier rotaclone and testpack rotavirus), and electron microscopy. a total of 249 stool specimens collected from children with gastroenteritis between february and april 1997 were tested. after resolution of 19 of the 22 discordant results by reverse transcription-pcr for group a rotavirus, ics-rv d ... | 1999 | 10325358 |
| evidence of high-frequency genomic reassortment of group a rotavirus strains in bangladesh: emergence of type g9 in 1995. | we characterized 1,534 rotavirus (rv) strains collected in bangladesh from 1992 to 1997 to assess temporal changes in g type and to study the most common g and p types using reverse transcription-pcr, oligonucleotide probe hybridization, and monoclonal antibody-based enzyme immunoassay. results from this study combined with our previous findings from 1987 to 1991 (f. bingnan et al., j. clin. microbiol. 29:862-868, 1991, and l. e. unicomb et al., arch. virol. 132:201-208, 1993) (n = 2,515 fecal s ... | 1999 | 10325342 |
| characterization by polymerase chain reaction of ruminant rotaviruses isolated in italy. | several group a rotaviruses isolated in italy from cattle, buffalos and goats were characterized by polymerase chain reaction assay for g- and p-type. g6 and p5 were the types most frequently recovered. the genotypes of buffalo and goat strains were similar to those of cattle isolates. | 1999 | 10322609 |
| rotavirus vaccines: development and use for the prevention of diarrhoeal disease. | rotavirus causes annually 600 000 deaths of young children worldwide, mainly in developing countries. the burden of rotavirus disease is significant also in developed countries and has not diminished with improved hygiene. natural protective immunity against severe rotavirus disease is built up during the first 2-3 years of life. likewise, studies with live attenuated oral rotavirus vaccines have shown that the majority of severe episodes of rotavirus diarrhoea are preventable by oral immunizati ... | 1999 | 10219717 |
| comparison of specific and random priming in the reverse transcriptase polymerase chain reaction for genotyping group a rotaviruses. | this study describes an approach to the molecular typing of rotaviruses which requires only a single rna extraction and reverse transcription (rt) reaction using random primers. random-primed rt provides complementary dna (cdna) which can be used not only for g- and p-typing polymerase chain reactions (pcr), but also for the detection of other rna viruses which may act as enteric pathogens. it is a sensitive and specific method that can detect 10 virus particles/ml of 10% faecal suspension provi ... | 1999 | 10204700 |
| characterization of group c rotaviruses associated with diarrhea outbreaks in feeder pigs. | feces and serum specimens were collected from three farms in michigan on which approximately 50-lb (8- to 9-week-old) pigs experienced diarrhea just after placement into all-in-all-out finishing barns. the clinical signs (profuse watery diarrhea lasting about 2 weeks and no vomiting) were similar on all farms, and the morbidity rate was high (ranging from 60 to 80%) but without mortality. eleven diarrheic fecal samples from the farms were tested for group a and c rotaviruses by immune electron m ... | 1999 | 10203510 |
| immunity to rotavirus infection in mice. | recent findings from our laboratory regarding the immune response of mice to rotavirus (a mucosal pathogen) show that although in most situations an acquired (t or b cell or both) response is necessary for elimination of primary rotavirus infection, unidentified innate mechanisms can also play a role in some mouse strains. similar to what is seen with many other viruses, cd8+ t cells appear to provide the first but not the exclusive mechanism that mediates clearance of a primary rotavirus infect ... | 1999 | 10099121 |
| viral diarrhea in children in beijing, china. | a study was undertaken from november 1994 to august 1996 to determine the role of viruses in children (< or =5 years of age) hospitalized at beijing children hospital, beijing china, for acute diarrhea. stool samples from diarrheal patients were investigated by elisa, electron microscopy, and rt-pcr for the presence of rotavirus, calicivirus, astrovirus, and adenovirus. group a rotavirus was detected in 55.9% of all diarrheal patients and comprised 82.5% of all viruses detected. group a rotaviru ... | 1999 | 10089052 |
| burden of human rotavirus-associated hospitalizations in three geographic regions of hungary. | data on hospital admissions and laboratory reports were used to estimate the number of hospitalizations of children aged 14 y or less in three geographic regions of hungary due to group a rotavirus infection. between january 1993 and december 1996, 9182 hospitalizations for gastroenteritis occurred, of which 1946 (21%) were associated with rotavirus infection. most (90%) of the rotavirus detections were among children aged 4 y or less. by extrapolation, an estimated 5000 rotavirus-related hospit ... | 1999 | 10088914 |
| seasonality and diversity of group a rotaviruses in europe. | group a rotaviruses are a major cause of severe gastroenteritis in children under 4 y of age worldwide. group a rotaviruses have been identified in many animal and bird species, they are antigenically complex, and multiple serotypes infect humans. re-assortant rotavirus vaccines are now available which confer protection against severe illness due to rotavirus serotypes g1-4. before vaccines are introduced it is necessary to establish the diversity of rotavirus in the target population to ensure ... | 1999 | 10088906 |
| rotavirus g-type restriction, persistence, and herd type specificity in swedish cattle herds. | g-typing of rotavirus strains enables the study of molecular epidemiology and gathering of information to promote disease prevention and control. rotavirus strains in fecal specimens from neonatal calves in swedish cattle herds were therefore characterized by using g1 to -4-, g6-, g8-, and g10-specific primers in reverse transcription (rt)-pcr. fecal samples were collected from one dairy herd (herd a) for 4 consecutive years and from 41 beef and dairy herds (herd b) experiencing calf diarrhea ou ... | 1999 | 10066651 |
| human group c rotavirus in children with diarrhea in the federal district, brazil. | group c rotaviruses are fastidious in their in vitro cell culture requirements. recent serosurveys indicate that antibody to group c rotavirus is present in 3-45% of the human population in certain geographic locations, suggesting that rotavirus group c infection is more prevalent than previously believed and that the low rate of detection of these agents is probably due to the lack of sensitive diagnostic assays. from march to december 1994, 406 fecal specimens were collected from children unde ... | 1998 | 9921274 |
| infection with group c rotavirus in a suburban community in brazil. | group c rotaviruses are associated with sporadic outbreaks of gastroenteritis worldwide. age-specific seroprevalence of group c rotavirus antibodies was investigated in sera, randomly collected and representative of a suburban community in brazil which had previously been screened for group a rotavirus antibodies. antibody prevalence to group c rotavirus was low in children under 5 years and increased slowly with age to 36% seropositivity in adults, reflecting continuous exposure to primary infe ... | 1998 | 9855402 |
| an outbreak of group c rotavirus gastroenteritis among adults living in valentim gentil, são paulo state, brazil. | an outbreak of gastroenteritis affecting adults and children occurred in the small city of valentim gentil, são paulo, brazil, in 1993. nineteen faecal samples (from 10 cases and 9 contracts) were examined by direct electron microscopy (dem), immune electron microscopy (iem), polyacrylamide gel electrophoresis (page), and enzyme-linked immunosorbent assay (elisa) for group a and c rotaviruses. dem detected rotavirus in 6 of the 10 cases and in none of the contacts. all of the samples were negati ... | 1998 | 9805410 |
| group a rotavirus as a cause of neonatal calf enteritis in sweden. | faeces samples were collected during outbreaks of neonatal calf diarrhoea in 14 beef and dairy herds. samples from 33 calves were taken at the onset of diarrhoea as well as from 30 calves with no signs of enteritis. no vaccines or medical treatment had previously been given. the mean age of the calves was 16.8 days (sd 8.2). the clinical evaluation of faeces consistency corresponded well to the dry matter content of the faeces (p < 0.001). the samples were analyzed for rotavirus, cryptosporidium ... | 1998 | 9787483 |
| rotavirus g and p types in children from belém, northern brazil, as determined by rt-pcr: occurrence of mixed p type infections. | fifty-four group a rotavirus-positive stool samples, obtained from children aged less than three years during a longitudinal (december 1982 to march 1986) study in belém, brazil, were re-examined. the samples were tested by reverse-transcription and polymerase chain reaction to determine their g-type and p-type specificity. only 17 (32%) of these rotavirus strains could be successfully g- and p-genotyped. while 10 (59%) of the 17 strains showed single g- and p-type specificity, the remaining bel ... | 1998 | 9775575 |
| enzyme-linked immunosorbent assay based on recombinant human group c rotavirus inner capsid protein (vp6) to detect human group c rotaviruses in fecal samples. | a recent study showed that 43% of a population in the united kingdom were seropositive for group c rotavirus. the higher than expected incidence may be due to limited diagnosis of acute human group c rotavirus infections because no routine test is available. human group c rotavirus infections are routinely diagnosed by electron microscopy (em) and a negative group a rotavirus enzyme-linked immunosorbent assay (elisa) result. an antigen-detection elisa was developed with hyperimmune antibodies ra ... | 1998 | 9774561 |
| development of a heterologous model in germfree suckling rats for studies of rotavirus diarrhea. | germfree suckling rats were infected with an sa11 rotavirus strain. infected pups developed diarrhea associated with histopathological changes. the virus was detected in feces and in the small intestine. cellular vacuolation was observed in the villi of the jejunum. these results provide a new model for further investigations of group a rotavirus infection. | 1998 | 9765478 |
| proportional morbidity rates of enteropathogens among diarrheic dairy calves in central spain. | faecal samples from 218 diarrheic dairy calves in 65 dairy herds, selected by convenience, were screened for the presence of rotavirus, coronavirus, cryptosporidium spp., f5+ escherichia coli and salmonella spp. animals surveyed were from 1 to 30 days old. cryptosporidium and rotavirus were the most commonly detected agents (52.3% and 42.7% of the samples positive, respectively). f5+ e. coli was detected in the faeces of 11.9% of the calves and bovine coronavirus was detected in the faeces of 7. ... | 1998 | 9762735 |
| epidemiological study of prevalence of genogroup ii human calicivirus (mexico virus) infections in japan and southeast asia as determined by enzyme-linked immunosorbent assays. | mexico virus (mxv) is a genogroup ii human calicivirus (hucv). we conducted an epidemiological study to determine the prevalence of mxv infection in infants and adults in japan and southeast asia by enzyme-linked immunosorbent assays (elisas) developed by using baculovirus-expressed recombinant mxv (rmxv) capsids. of 155 stool specimens obtained from children younger than 10 years old with acute clinical gastroenteritis (diarrhea and vomiting) associated with small, round-structured viruses in j ... | 1998 | 9705378 |
| seroepidemiology of group a rotavirus in suburban são paulo, brazil. | age-specific patterns of rotavirus infection were investigated using a randomly selected and representative sample of sera from a suburban community of são paulo, brazil screened for class-specific antibodies to group a rotavirus. age-serology of anti-rotavirus igg showed primary infection predominant in young infants with a median age of around 18 months consistent with igm serology suggesting highest rates of recent infection between ages 4 and 48 months. anti-rotavirus serum iga prevalence in ... | 1998 | 9692612 |
| serotypic characterization of outer capsid spike protein vp4 of vervet monkey rotavirus sa11 strain. | the vervet monkey rotavirus sa11, a prototype strain of group a rotaviruses, has been shown to possess vp7 serotype 3 specificity but its neutralization specificity with regard to the other outer capsid protein vp4 has not been elucidated. we thus determined its vp4 specificity by two-way cross-neutralization with guinea pig antiserum prepared with a single gene substitution reassortant that had only the vp4-encoding gene from the simian rotavirus sa11 strain and remaining ten genes from human r ... | 1998 | 9687880 |
| distribution of g serotypes and p genotypes of bovine group a rotavirus isolated in japan. | to identify the distribution of g serotype and p genotype of bovine group a rotavirus in japan. | 1998 | 9673768 |
| nigerian rotavirus serotype g8 could not be typed by pcr due to nucleotide mutation at the 3' end of the primer binding site. | a rotavirus strain hmg89 from nigeria with short electrophoretic pattern was typed g3 by pcr. a cdna clone from the pcr product which hybridised in northern blots to rna segment 9 of the homologous nigerian rotavirus strain hmg89 and laboratory reference strain 69m but not to other mammalian group a rotaviruses was sequenced. the vp7 gene 9 sequence is 1060 nucleotides long with two base deletions at positions 1034-1035. sequence analysis of the primer (aat8) used in the previous pcr serotyping ... | 1997 | 9672646 |
| conserved structural features of nonstructural glycoprotein nsp4 between group a and group c rotaviruses. | the nonstructural glycoprotein nsp4 of group c human rotavirus strain ehime 9301 was determined to be 150 amino acids in length and 96% identical with the nsp4 of another group c human rotavirus strain bristol. both nsp4 sequences were virtually unrelated to group a rotavirus nsp4s. however, the structural features of group a and group c rotavirus nsp4s were similar with hydrophobic domains being in the amino terminus and a coiled coil domain after the membrane-spanning domain, although group c ... | 1997 | 9672644 |
| molecular and immunologic characterization of group a bovine rotavirus field isolates with p8[11] spike protein. | bovine rotavirus strain b223 is the north american prototype for group a p8[11] serotype/genotype rotaviruses. rotaviruses with this serotype/genotype are a prevalent cause of neonatal calf diarrhea and have also been isolated from asymptomatic human infants. on the basis of deduced amino acid sequence of the outer capsid viral protein 4 (vp4), strain b223 lacks a cysteine at position 318 that is conserved among all other rotavirus strains. it has been speculated that this may result in the loss ... | 1998 | 9645207 |
| the role of environmental contamination with small round structured viruses in a hospital outbreak investigated by reverse-transcriptase polymerase chain reaction assay. | in may 1994 an outbreak of vomiting and diarrhoea occurred in a 28-bed long-stay ward for the mentally infirm. the predominant symptoms were vomiting, diarrhoea, malaise and abdominal pain lasting for approximately 12 h in most cases. the attack rate was 62% (13/21) for patients and 46% (16/35) for staff members. infection control measures were implemented (containment of infectious individuals, hand hygiene among staff and environmental decontamination) and the ward was closed to admissions. af ... | 1998 | 9617683 |
| serological and genomic characterization of human rotaviruses detected in china. | a total of 1,385 stool specimens were collected from children with diarrhea at two hospitals in wuhan, hubei province, china, in 1994 and 1995, and screened for rotavirus by polyacrylamide gel electrophoresis of viral rna. group a rotavirus was detected with high frequency; 56.5% (87/154) and 40.8% (502/1,231) of the specimens collected in 1994 and 1995, respectively, were positive for rotavirus. assignment of g serotype and p type (vp4 genotype) of group a rotavirus by elisa with monoclonal ant ... | 1998 | 9598939 |
| rotavirus vaccines. | encouraging results have been reported from several large trials of tetravalent rhesus rotavirus vaccine, with efficacy of 70-80% against severe disease. a recent venezuelan study showed similar results to trials in usa and europe. the vaccine may soon be licensed in usa. it provides the exciting prospect of a strategy to prevent one of the world's major child killers. other candidate vaccines are under development including human-bovine reassortants, neonatal strains, non-replicating rotaviruse ... | 1998 | 9553287 |
| assembly of viroplasm and virus-like particles of rotavirus by a semliki forest virus replicon. | in this study we have used an expression system based on semliki forest virus (sfv) to study assembly and intracellular localization of certain capsid proteins of rotavirus in neurons and mammalian epithelial cells. the complete genes of vp2 (vp2a) and vp6 (vp6a) of group a rotavirus (sa-11) and gene 5 encoding vp6 (vp6c) of porcine group c rotavirus (strain cowden/amc-1) were inserted into an sfv expression replicon. transfection of bhk-21 cells with in vitro-made sfv transcripts resulted in a ... | 1998 | 9514960 |
| short sequences define genetic lineages: phylogenetic analysis of group a rotaviruses based on partial sequences of genome segments 4 and 9. | genetic diversity in strains of human group a rotaviruses was analysed by phylogenetic methods. the study material comprised 109 serotype g1 or g4 rotavirus samples isolated in finland during 1986-1990. parts of the coding regions of rotaviral genome segments 4 and 9, which encode proteins with serotype specificity, the spike protein vp4 (p serotype) and the outer capsid protein vp7 (g serotype), respectively, were sequenced. as determined by analysis of segment 4 sequences all g1 strains and al ... | 1998 | 9472616 |
| prevalence of human group a rotavirus serotypes in pune, india (1990-1993). | a total of 205 specimens positive for rotaviruses obtained during 1990-93 surveillance studies of hospitalized patients with diarrhoea were tested by elisa for g serotypes. of these, 107 (52.19%) specimens could be serotyped. seventy four specimens were assigned to one of the g1-g4 serotypes. of the 74 specimens, 49 (66.2%) reacted with mab against g2. of 107 typeable specimens, 33 showed dual or multiple reactivity. eighteen specimens reacted with mab specific to g1 and g2 serotypes. five speci ... | 1997 | 9439097 |
| etiology of acute gastroenteritis in hospitalized children in melbourne, australia, from april 1980 to march 1993. | acute infectious diarrhea is common in children. control requires knowledge of causes. few comprehensive long-term studies of etiology have been undertaken in developed countries. this report is of a 13-year survey of 4,637 children from 0 to 14 years of age, admitted to a large children's hospital for treatment of gastroenteritis, in which viruses, bacteria, and parasites were sought. a recognized enteric pathogen was identified in 56.6% of children. group a rotaviruses occurred in 39.6% of chi ... | 1998 | 9431936 |
| survey of human group c rotaviruses in japan during the winter of 1992 to 1993. | fecal specimens from patients with acute diarrhea were collected from 10 prefectures in japan over a 6-month period (november 1992 to april 1993), and the specimens that were negative for human group a rotaviruses were screened for the presence of human group c rotaviruses (chrvs) by the reverse passive hemagglutination test. of 784 specimens examined, 53 samples (6.8%) that were collected in 7 of 10 prefectures were positive for chrv, indicating that chrvs are widely distributed across japan. m ... | 1998 | 9431910 |
| comparison of electron microscopy, enzyme-linked immunosorbent assay and latex agglutination for the detection of bovine rotavirus in faeces. | the performance characteristics of 2 enzyme immunoassays (elisas) and 4 latex agglutination assays (lxs) were evaluated for the detection of bovine rotavirus in faecal specimens of young calves with diarrhoea. a total of 26 specimens from calves less than 5 months of age were examined with different commercial assays and compared with electron microscopy (em) as the gold standard and with polyacrylamide gel electrophoresis (page) for the detection of atypical, non-group a rotaviruses. in the 2nd ... | 1997 | 9408894 |
| comparison of enzyme immunoassay, pcr, and type-specific cdna probe techniques for identification of group a rotavirus gene 4 types (p types). | this study was designed to evaluate three techniques most commonly used to identify the vp4 (p) types of human group a fecal rotaviruses. the techniques included pcr with nested primers and hybridization with pcr-generated probes (to determine the p genotypes). the results obtained by these genetic techniques were evaluated against those obtained by an enzyme immunoassay (eia) incorporating neutralizing monoclonal antibodies (n-mabs) reacting with three major human p serotypes (serotypes p1a, p1 ... | 1997 | 9399502 |
| restriction endonucleases whose sites are predictable from the amino acid sequence offer an improved strategy for typing bovine rotaviruses. | variation in the third base of a codon hampers genotypic characterization, particularly of rna viruses. some restriction endonucleases, however, have a recognition site with a variable base at the third position and will always cleave when a certain amino acid pair occurs (such as glycine-proline for sau96i and glutamic or aspartic acid followed by serine usually for hinfi). we developed a restriction fragment length polymorphism (rflp) procedure based on these enzymes for p-typing bovine group ... | 1997 | 9375295 |
| serotyping of group a rotaviruses in egyptian neonates and infants less than 1 year old with acute diarrhea. | group a human rotavirus g serotypes were detected in stool specimens from neonates and infants with and without acute diarrhea in cairo by using monoclonal antibodies in an enzyme-linked immunosorbent assay. serotypes g1 and g4 predominated in all age groups. mixed (g1 plus g4) and nontypeable specimens represented 16.1 and 38.7% of the total number serotyped, respectively. | 1997 | 9350778 |
| rna profile and structural protein analysis of rotaviruses isolated from diarrhoeal calves in india. | two isolates of group a rotaviruses (cr129 and cr156) were isolated from faecal samples of diarrhoeal calves reared in two dairy farms at hisar (haryana, india) by using ma-104 cell lines. these isolates were compared with three standard reference bovine rotaviruses, uk, ncdv and b223, to reveal differences, if any, in their genome and protein migration profiles. the migration of rna segment 4 of cr129 was slower than that of ncdv, but faster than that of uk. segment 10 of cr156 moved faster tha ... | 1997 | 9308295 |
| production of reassortant viruses containing human rotavirus vp4 and sa11 vp7 for measuring neutralizing antibody following natural infection. | the outer capsid proteins vp4 and vp7 of group a rotaviruses are both targets of neutralizing antibody produced following natural infection in humans. of interest is the relative importance and immunodominance of each protein in the generation of a protective immune response. in order to measure neutralizing antibody responses to vp4 and vp7 separately, reassortants bearing vp4 of each of the major human rotavirus p types with vp7 of sa11 origin were successfully produced by neutralizing monoclo ... | 1997 | 9302196 |
| isolation and identification of avian rotavirus from pheasant chicks with signs of clinical enteritis. | three rotaviruses were isolated from intestinal contents obtained from a flock of 6-8-day-old pheasant chicks showing diarrhoea and increased mortality. the isolates were characterized as avian group a rotavirus by immunoenzymatic technique (elisa) and polyacrylamide gel electrophoresis (page). | 1997 | 9280387 |
| identification of bovine rotaviruses in venezuela: antigenic and molecular characterization of a bovine rotavirus strain. | two serotypes of bovine group a rotaviruses were demonstrated by enzyme-linked immunoassay (elisa) and polyacrylamide gel electrophoresis (page) in 20 of 171 faecal samples collected from diarrhoeic calves in two dairy farms in venezuela. by serotyping elisa using g and p serotype-specific monoclonal antibodies, bovine rotaviruses (brv) circulating on one farm were identified as serotype g6, while brvs circulating on the other farm were identified as serotype g10. only one brv (033) could be suc ... | 1997 | 9272580 |
| new aspects in a murine model for extrahepatic biliary atresia. | the cause of extrahepatic biliary atresia (ehba) remains unknown, and even an animal model is still lacking. observations in a murine infectious model (newborn balb/c mice infected with rhesus rotavirus group a [rrv]) reported extrahepatic biliary obstruction similar to findings in children who have ehba. in the present study, this animal experiment was repeated, and the clinical and histomorphologic changes were observed over 3 weeks. eighty-nine newborn mice were infected with rrv, and 67% sho ... | 1997 | 9269968 |
| protective immunity induced by rotavirus dna vaccines. | it is estimated that group a rotavirus diarrhea causes as many as one million deaths per year in children worldwide, and effective vaccines will be essential for their control. plasmid dna vaccines encoding murine rotaviral proteins vp4, vp6, or vp7 were tested in adult balb/c mice for their ability to induce immune responses and provide protection against rotavirus challenge. the vaccines were administered by inoculation into cells of the epidermis with an accell gene gun. (auragen, inc., middl ... | 1997 | 9234543 |
| isolation and characterization of cytopathic strains of rotavirus from hares (lepus europaeus). | ten cytopathic rotavirus were recovered from stools of leverets affected by an enteric syndrome. the ten isolates, examined by means of elisa and sds-pa-ge, were identified as group a rotaviruses. | 1997 | 9208423 |
| characterization of a porcine enterocyte receptor for group a rotavirus. | we have identified, purified to apparent homogeneity and chemically characterized a biologically-relevant porcine enterocyte receptor for group a porcine rotavirus. ceramide glycanase digestion followed by acid hydrolysis and monosaccharide compositional analyses indicated the receptor is a family of two gm, gangliosides, one containing n-glycolyl-neuraminic acid and the other n-acetylneuraminic acid. both gangliosides displayed dose-dependent inhibition of rotavirus binding to, and infectivity ... | 1997 | 9192005 |
| identification of a new genogroup of aquareovirus by rna-rna hybridization. | the relative mobilities of the 11 dsrna genomic segments of 22 aquareovirus isolates from fish and shellfish obtained from different geographical areas of the world were compared by page. using reciprocal rna-rna dot blot hybridization, a new sixth genetic group of aquareovirus (genogroup f) was identified. genogroup a was represented by eight and genogroup b by 12 isolates. the remaining two isolates represented the new sixth genogroup (genogroup f). the genetic relationship of these aquareovir ... | 1997 | 9191934 |
| prevalence and characteristics of severe rotavirus infections in nicaraguan children. | we analyzed the prevalence of rotavirus in 296 children age between 3 and 36 months who were hospitalized in 1994 with severe gastro-enteritis at two health centres for diarrhoea treatment in león, nicaragua. enteric viruses were detected in 96 (32.4%) of the children and rotaviruses were the most common pathogens detected in 84 (28%). the majority of rotavirus infections occurred in children less than 1 year old and all strains isolated belonged to subgroup ii and had 'long' rna patterns. molec ... | 1997 | 9176574 |
| seroepidemiology of human group c rotavirus in the uk. | the gene coding for the major inner capsid protein vp6 of human group c rotavirus was cloned into baculovirus using the pbluebac2 vector and expressed in insect cells. when cultured in high five cells, vp6 was expressed at a high level and exported to the cell culture medium. purified vp6 was used to immunise rabbits. hyperimmune rabbit serum, which reacted with native human group c rotavirus in infected cells, was used to develop and optimise an eia for the detection of antibodies to group c ro ... | 1997 | 9131463 |
| isolation of serotype g8, p6[1] bovine rotavirus from adult cattle with diarrhea. | two electrophoretically identical strains of group a rotavirus were isolated from diarrheic cows aged 3, 4, and 7 years on a dairy farm in japan. they had a rare serotype combination of g8 and p6[1] and were shown by rna-rna hybridization to be most closely related to bovine strain ncdv-lincoln (g6p6[1]). | 1997 | 9114422 |
| rapid vertical agarose: silver stain detection of rotavirus. | human rotaviruses (hrv) are the most important etiologic agents of acquired diarrhea in infants and young children worldwide. therefore, the early diagnosis is essential for effective patient management and infection control. we have developed a rapid, simple technique for the diagnosis of rotavirus based on the sensitive detection of rotavirus double-stranded rna genome segments separated in vertical agarose gels and developed by silver staining (age-ss). this method also has the ability to det ... | 1997 | 9095532 |
| lack of evidence for rotavirus by polymerase chain reaction/enzyme immunoassay of hepatobiliary samples from children with biliary atresia. | the purpose of this study was to analyze hepatobiliary samples of patients with biliary atresia for rotavirus groups. a, b, and c, because group a rotavirus had been used to produce an animal model of the disease and group c rotavirus had been found in hepatobiliary samples from one group of patients. biliary remnants and liver tissue from 10 biliary atresia and 14 control patients with other liver diseases were examined for rotavirus groups a, b, and c using nonisotopic, reverse transcriptase p ... | 1997 | 9029644 |
| characterization of rotaviral rna isolated from children with gastroenteritis in poland. | rotaviruses were detected in 37% (331/895) of stool specimens collected from children with gastroenteritis attending three hospitals in warsaw between january 1981 and march 1994. an analysis of rotavirus electrophoretypes circulating in warsaw and cracow, poland, over 6 years showed that all 176 electrophoretypes encountered were characteristic of group a rotaviruses. based on variations in the migration pattern of rna genome segments when passed through polyacrylamide gels, 10 different electr ... | 1996 | 9017144 |
| prophylaxis of rotavirus gastroenteritis using immunoglobulin. | oral inoculation of the human group a rotavirus mo strain (g serotype 3) into 5-day-old balb/c mice causes gastroenteritis characterized by diarrhea. using this small animal model, passive protection of suckling mice against human rotavirus infection was achieved with the use of immunoglobulin (igy) from the yolks of eggs of rotavirus-immunized hens. when igy against the rotavirus strain homotypic with the challenge virus (mo strain) was administered to mice, complete protection was achieved. af ... | 1996 | 9015118 |
| protective immunity against group a rotavirus infection and illness in infants. | understanding of the protective effect provided by natural rotavirus infections against subsequent rotavirus infections is required for evaluating vaccine development programs. prior studies of the protective efficacy of natural infections and correlates of natural protection are reviewed and results from several studies presented only in abstract form are summarized to provide a current assessment of knowledge in this area. six cohort studies have reported rates for the protective efficacy of a ... | 1996 | 9015110 |
| natural history of human rotavirus infection. | rotavirus infections occur repeatedly in humans from birth to old age. most are asymptomatic or are associated with mild enteric symptoms. infection in young children can be accompanied by severe life-threatening diarrhea, most commonly after primary infection. annual childhood morbidity rates for severe diarrhea are similar worldwide. mortality rates are low in developed countries but approach 1,000,000 annually in young children in developing countries. rotaviruses can be classified into group ... | 1996 | 9015109 |
| identification of a t-helper cell epitope on the rotavirus vp6 protein. | in this work, we have studied the t-helper (th)-cell response against rotavirus, in a mouse model. adult balb/c mice were inoculated parenterally with porcine rotavirus ym, and the th-cell response from spleen cells against the virus and two overlapping fragments of the major capsid protein vp6 (vp6(1-192) and vp6(171-397)) were evaluated in vitro. the th cells recognized the ym virus and the two protein fragments, suggesting that there are at least two th-cell epitopes on the vp6 molecule. to s ... | 1997 | 8985366 |
| the vp3 gene of human group c rotavirus. | the complete nucleotide sequence of genome segment 4 from the human group c rotavirus (bristol strain) was determined. comparison of the nucleotide sequences of the genome termini with the consensus 5' and 3' terminal non-coding sequences of the human group c rotavirus genome revealed characteristic 5' and 3' sequence motifs. human group c rotavirus genome segment 4 is 2,166bp long and encodes a single open reading frame of 2,082 nucleotides (693 amino acids) starting at nucleotide 55 and termin ... | 1996 | 8972570 |
| evaluation of reverse transcription and polymerase chain reaction (rt/pcr) for the detection of rotaviruses: applications of the assay. | our aim was to evaluate the reverse transcription and polymerase chain reaction (rt/pcr) technique for the detection of rotavirus shedding by infected children as a routine diagnostic procedure, in comparison to the enzyme-linked immunosorbent assay (elisa), electron microscopy (em) and polyacrylamide gel electrophoresis (page) of rotavirus double-stranded rna. two-hundred and twenty stool specimens were collected from infants and young children with diarrhoea, and 10-20% faecal suspensions were ... | 1996 | 8958588 |
| genetic stability of a porcine rotavirus rna segment during repeated plaque isolation. | the porcine group a rotavirus cc86 was characterized to explore its utility as a tool for mutation analysis. it has a semiduplication of the gene 11 rna segment. nucleotide sequence determination of cdna confirmed that the nsp5 coding sequence and the conserved nontranslated termini of the rna segment were retained. a comparison of the nsp5 genes of cc86 and cn86 that were isolated from the same fecal specimen showed eight base pair changes, suggesting that cn86 was not the immediate progenitor ... | 1996 | 8918545 |
| the characterization of vp7 (g type) and vp4 (p type) genes of bovine group a rotaviruses from field samples using rt-pcr and rflp analysis. | characterization of the vp7 (g type) and vp4 (p type) genes of bovine group a rotaviruses (brv) from field samples was performed using rt-pcr and rflp analysis. after rt-pcr amplification of the full length vp7 genes and partial length vp4 genes (nucleotides 1 to 1096), four enzymes, ecorv, nlaiv, bamhi and hpaii were used for digestion analysis. for vp7, four rflp profiles were observed after analysis of the digests: they were designated as g6, g6s (subtype, showed about 86% nucleotide and 90% ... | 1996 | 8893794 |
| the 3'-terminal consensus sequence of rotavirus mrna is the minimal promoter of negative-strand rna synthesis. | we used an in vitro template-dependent replicase assay (d. chen, c. zeng, m. wentz, m. gorziglia, m. estes, and r. ramig. j. virol. 68:7030-7039, 1994) to identify the cis-acting signals required for replication of a genome segment 9 template from the group a rotavirus strain osu. the replicase phenotypes for a panel of templates with internal deletions or 3'-terminal truncations indicated that no essential replication signals were present within the open reading frame and that key elements were ... | 1996 | 8892905 |
| outbreak of severe gastroenteritis in adults and children associated with type g2 rotavirus. study group on diarrhea of the instituto adolfo lutz. | an outbreak of severe gastroenteritis affecting 132 adults and children occurred in the small city of mirassol, são paulo, brazil, in 1992. the outbreak of diarrhoeal disease had an abrupt onset and afflicted all age segments of the population. group a rotavirus was the only pathogen associated with the epidemic. it was detected in 12 of the 27 (44%) stool specimens analyzed and was identified as serotype g2 rotavirus. severe dehydration was common among adults and older children, and 35% of all ... | 1996 | 8870397 |
| subgroup determination of group a rotaviruses recovered from piglets in nigeria. | subgroup analysis using subgroup-specific monoclonal elisa revealed a preponderance of subgroup 2-specific antigens of group a porcine rotaviruses over subgroup 1. of 113 polyacrylamide gel electrophoresis-positive test samples, obtained from 4 states of nigeria, 31 (27.4%) and 45 (39.8%) were determined to have subgroup 1 and 2 specificities, respectively. however, 37 (32.7%) test samples could not be classified into any of the known group a rotavirus subgroups. these "unclassifiable" samples p ... | 1995 | 8833268 |
| enterotoxigenic escherichia coli, rotavirus, porcine epidemic diarrhoea virus, adenovirus and calici-like virus in porcine postweaning diarrhoea in hungary. | in order to obtain data about the significance of enterotoxigenic e. coli (etec), and of the different enteric viruses in the aetiology of porcine postweaning diarrhoea, bacteriological, electron microscopic and elisa studies were made on dead and live pigs. e. coli from the small intestine of diarrhoeal weaned pigs that died were tested for serogroups, pili (fimbriae) and toxin-geno-type. the 108 haemolytic e. coli representing 14 farms and 42 pigs were typed as follows: 0149:k88+etec (56.5%), ... | 1996 | 8826697 |
| diversity of rotavirus strains infecting pediatric patients in new delhi, india. | polyacrylamide gel electrophoresis (page) of rotavirus can provide information on variation in rotavirus strain prevalent in the community. in the present study 157 samples were collected from children below 5 years of age presenting with acute diarrhoea from may to december, 1990 at safdarjung hospital, new delhi. seventy-one (45 percent) of these stool samples were positive for rotavirus by elisa. sixty-seven samples showed discernible rna pattern of group a rotavirus by page. it was found tha ... | 1996 | 8816032 |
| phosphorylation generates different forms of rotavirus nsp5. | nsp5 (non-structural protein 5) is one of two proteins encoded by genome segment 11 of group a rotaviruses. in virus-infected cells nsp5 accumulates in the virosomes and is found as two polypeptides with molecular masses of 26 and 28 kda (26k and 28k proteins). nsp5 has been previously shown to be post-translationally modified by the addition of o-linked monosaccharide residues of n-acetylglucosamine and also by phosphorylation. we have now found that, as a consequence of phosphorylation, a comp ... | 1996 | 8811003 |
| unusual diversity of human rotavirus g and p genotypes in india. | between april and december 1993, we determined p and g genotypes of group a rotavirus strains obtained from children admitted to diarrhea treatment centers in five indian cities. from a total of 63 rotavirus-positive specimens, we identified 10 different strains with five different g genotypes and four distinct p types by using reverse transcription-pcr. the common worldwide strains g1p8, g2p4, g3p8, and g4p8 were underrepresented among indian children (33%), whereas strains of p type 6 (g1p6, g ... | 1996 | 8789033 |
| role of enteric pathogens in the aetiology of neonatal diarrhoea in lambs and goat kids in spain. | faeces samples from diarrhoeic and non-diarrhoeic lambs and goat kids aged 1-45 days were examined for enteric pathogens. cryptosporidium parvum was detected in both diarrhoeic lambs (45%) and goat kids (42%) but not in non-diarrhoeic animals. f5+ (k99+) and/or f41+ escherichia coli strains were isolated from 26% and 22% of the diarrhoeic lambs and goat kids, respectively, although these strains, which did not produce enterotoxins st i or lt i, were found with similar frequencies in non-diarrhoe ... | 1996 | 8760970 |
| group a rotavirus veterinary vaccines. | group a rotaviruses cause diarrhea in young livestock and poultry; consequently, vaccination strategies have focused on induction of active or passive immunity. gnotobiotic pigs and calves serve as useful models to evaluate induction of active immunity by candidate animal or human rotavirus vaccines. however, live attenuated rotavirus vaccines lacked efficacy when administered orally to calves and pigs in the field, presumably because colostral antibodies inhibited vaccine virus replication. the ... | 1996 | 8752298 |
| efficacy of a quadrivalent rhesus rotavirus-based human rotavirus vaccine aimed at preventing severe rotavirus diarrhea in infants and young children. | the most extensively explored strategy for rotavirus vaccination has been the jennerian approach, which uses an antigenically related rotavirus strain from an animal host as the immunogen to induce protection against the 4 epidemiologically important group a rotavirus vp7 serotypes. because this approach has shown limited efficacy, a modified jennerian approach was developed with the goal of achieving broader antigenic coverage. four vp7 serotypes were incorporated into a quadrivalent vaccine co ... | 1996 | 8752293 |
| hospital admissions attributable to rotavirus infection in england and wales. | laboratory reports and data on hospital admissions were used to estimate the number of hospitalizations due to group a rotavirus infection in england and wales. between january 1990 and december 1994, there were 75,059 laboratory reports of rotavirus infection, and 66,062 of these were in children <5 years old; rotavirus represented 39% of all pathogens identified in fecal specimens from this age group. between april 1993 and march 1994, 1904 hospital admissions coded as "infectious intestinal d ... | 1996 | 8752285 |
| evaluation of a one-step test for rapid, in practice detection of rotavirus in farm animals. | an immunochromatographic test for the detection of group a rotavirus was evaluated against a reference group a rotavirus elisa, by using a panel of 161 bovine, porcine and equine faecal samples submitted for routine examination. the sensitivity of the test was 89 per cent and the specificity 99 per cent compared with the elisa. its reproducibility was 100 per cent. the simplicity and rapidity of the test procedure make it suitable for use in practice. | 1996 | 8732193 |
| demonstration of a lack of synergistic effect of rotavirus with other diarrheal pathogens on severity of diarrhea in children. | the severity of group a rotavirus (rv) diarrhea was compared with that of mixed infections of rv with diarrheagenic escherichia coli, vibrio cholerae o1, and shigella species by a scoring system. the severity of mixed infections of rv and e. coli was the same as that of infections with rv alone. rv infections mixed with v. cholerae and shigella species mimicked cholera and shigellosis, respectively. | 1996 | 8727937 |
| serological and genomic characterization of porcine rotaviruses in thailand: detection of a g10 porcine rotavirus. | a total of 557 fecal specimens collected from piglets with diarrhea in thailand were examined for rotavirus rna by polyacrylamide gel electrophoresis. twenty-three, one, and two samples were positive for group a, group b, and group c rotaviruses, respectively. two samples exhibited two segments found in picobirnavirus rna. rna electropherotyping of 23 group a rotaviruses showed that they were classified into five patterns. by serotyping by enzyme-linked immunosorbent assay and pcr, viruses in 3 ... | 1996 | 8727874 |
| electropherotypes of rotavirus strains causing gastroenteritis in infants and young children in tirana, albania, from 1988 to 1991. | during 1988-1991, an epidemiological survey was conducted in tirana (albania) on group a rotavirus strains which cause gastroenteritis in infants and young children. rotaviruses were detected in 312 of 1,241 (25.1%) examined specimens from children with acute diarrhoea. viruses were detected throughout the study period. among the 72 rotavirus strains tested for double-stranded rna (dsrna) electrophoretic migration pattern, 9 different electropherotypes were recognized, 1 of those being more freq ... | 1995 | 8722294 |
| caprine and bovine b rotaviruses in western france: group identification by northern hybridization. | in a survey of coronavirus and rotavirus-induced neonatal diarrhoea in 373 calves from 284 farms, nine (2.4%) of the animals were found to be infected with non-group a rotaviruses when their faeces were analysed in a commercial elisa and by page. seven out of eight 1-4- day-old kids from a single farm were also infected with similar viruses. in a mini-page, all the viruses displayed group b- and/or e-like 4223 electrophoretypes. in northern hybridizations with cdna chemiluminescent probes specif ... | 1996 | 8721296 |
| development of mucosal and systemic lymphoproliferative responses and protective immunity to human group a rotaviruses in a gnotobiotic pig model. | gnotobiotic pigs were orally inoculated with virulent wa strain (g1p1a[8]) human rotavirus (group 1), attenuated wa rotavirus (group 2) or diluent (controls) and were challenged with virulent wa rotavirus 21 days later. on various postinoculation or postchallenge days, virus-specific responses of systemic (blood and spleen) and intestinal (mesenteric lymph node and ileal lamina propria) mononuclear cells (mnc) were assessed by lymphoproliferative assays (lpa). after inoculation, 100% of group 1 ... | 1996 | 8705681 |
| role of maternally derived circulating antibodies in protection of neonatal swine against porcine group a rotavirus. | the ability of maternally derived antibodies in the circulation of neonatal pigs to protect against challenge with virulent porcine group a rotavirus (osu strain) was evaluated. groups of neonatal pigs with nondetectable (group 1), low (group 2), or high (group 3) serum levels of osu rotavirus-specific maternally derived antibodies were challenged with virulent osu rotavirus at 3 days of age and monitored for infection and disease. control pigs were sham-inoculated with diluent at 3 days of age. ... | 1996 | 8699055 |
| a molecular epidemiological study of porcine rotaviruses. | diarrhoea in the piglet can lead to stunted growth and neonatal death, with important implications for the pig farmer. during 1993, a pilot study was initiated to monitor the epidemiology of rotavirus infection in piglets with diarrhoea. bimonthly diarrhoeal faecal specimens were collected from scouring piglets less than 6 weeks of age on a pig farm in the northern transvaal. the stool specimens were examined by a commercial enzyme-linked immunosorbent assay (rotavirus eia, idl, jerusalem). in t ... | 1995 | 8691407 |
| isolation and subgrouping of rotaviruses from buffalo calves in sri lanka. | twenty-eight faecal specimens from sri lankan buffalo calves shown to be positive for rotavirus group a antigen were subgrouped by an enzyme-linked immunosorbent assay, by using monoclonal antibodies prepared against subgroup i and ii antigens. the 13 of the 28 specimens which were classified as strongly positive belonged to subgroup i. three of five faecal specimens inoculated on to roller cultures of ma104 cells yielded group a subgroup i rotavirus. as with other group a rotaviruses isolated f ... | 1996 | 8685545 |
| an epidemiological study on viral infantile diarrhoea in tirana. | during the period may 1993-april 1994, an epidemiological survey was conducted on enteric viruses which cause gastroenteritis in infants and young children in tirana, albania. specimens from 321 cases were screened by direct electron microscopy and by an enzyme-linked immunosorbent assay specific for rotavirus group a antigen. by ultrastructural analysis, rotaviruses were detected in 10.3% of cases and adenoviruses in 0.6%, whereas small round structured viruses and small round viruses were foun ... | 1996 | 8673857 |
| hyperimmune cow colostrum reduces diarrhoea due to rotavirus: a double-blind, controlled clinical trial. | the therapeutic efficacy of hyperimmune bovine colostrum (hbc) from cows immunized with four serotypes of human rotavirus was evaluated in a double-blind, randomized trial in 75 boys, aged 6-24 months, infected with rotavirus diarrhoea. the treatment group received 100 ml of hbc three times a day for 3 consecutive days, while the controls received the same amount of bovine colostrum from significantly shorter duration of diarrhoea than the controls (median 56 versus 72 h (p<0.001); confidence in ... | 1995 | 8652974 |
| sequence comparison of the vp7 gene encoding the outer capsid glycoprotein among animal and human group c rotaviruses. | the nucleotide sequences of the outer capsid glycoprotein (vp7) genes from the shintoku bovine and the hf and wh porcine group c rotaviruses were determined and compared with those of the published corresponding genes from the cowden porcine and ehime human group c rotaviruses. the vp7 genes of all 5 strains were 1063 nucleotides in length and possess one open reading frame encoding a polypeptide of 332 amino acids. comparative analysis of the deduced amino acid sequences indicated that 85.2-97. ... | 1996 | 8645106 |
| cross-reactive, serotype- and monotype-specific neutralization epitopes on vp7 of serotype g3 and g5 porcine rotavirus strains. | vp7 specific monoclonal antibodies raised against serotype g5 porcine rotavirus strains isolated in venezuela showed either a serotype g5- or monotype-specific pattern of reactivity by neutralization against a panel of 53 group a rotavirus isolates representative of all established g serotypes. monoclonal antibodies raised against two g3 porcine strains were either specific for a subset of porcine g3 strains or reactive with another subset of porcine g3 strains and with most g5 strains. neither ... | 1996 | 8645098 |
| genetic and antigenic characterization of a serotype g6 human rotavirus isolated in melbourne, australia. | an unusual rotavirus strain, mg6, was isolated from a 16-month-old child admitted to hospital with acute gastroenteritis. the virus could not be serotyped (g-typed) by enzyme immunoassay using standard reagents specific for common serotypes of human group a rotaviruses. nucleotide sequencing of cdna derived from the gene encoding the outer capsid protein, vp7, and deduction of the vp7 amino acid sequence indicated that this strain belonged to serotype g6, a serotype normally associated with viru ... | 1995 | 8636702 |
| sensitive detection of group a rotaviruses by immunomagnetic separation and reverse transcription-polymerase chain reaction. | an immunomagnetic separation (ims) method was developed for concentrating rotaviruses from environmental samples, as well as a reverse transcription-polymerase chain reaction (rt-pcr) for the sensitive and specific detection of group a rotaviruses. magnetic beads were coated with monoclonal antibodies directed against the group-specific, inner capsid protein (vp6) and subsequently used to capture and purify the virus with the help of a magnet. the genome was made available for rt by heat-disrupt ... | 1995 | 8609198 |
| an unusual group-a rotavirus associated with an epidemic of diarrhea among three-month-old calves. | during the 1992 and 1993 breeding seasons, epidemics of diarrhea among calves approximately 3 months old in a cow/calf operation were reported. rotavirus was determined to be the probable cause, but because rotavirus typically affects younger calves, further investigations were conducted to determine the characteristics of the virus. virus isolated from the feces of 1 affected calf was found to be antigenically distinct from the vaccine strain used. the primary water source was a slough, and rap ... | 1996 | 8603906 |
| subclasses of iga antibodies in serum and saliva samples of newborns and infants immunized against rotavirus. | little is known about subclass levels of iga in serum or saliva of infants in the perinatal period. we have previously shown that very young infants are capable of responding to an experimental rotavirus vaccine with both serum and salivary iga, and that small amounts of iga are already detectable in cord blood of these infants. in the present study, total iga1 and iga2 antibodies in serum and saliva samples of some of these infants at birth, at 6 weeks of age, and at 12 weeks of age, were deter ... | 1996 | 8565301 |
| molecular characterization of the 11th rna segment from human group c rotavirus. | the complete nucleotide sequence of genome segment 11 from the noncultivatable, human group c rotavirus (bristol strain) was determined. comparison of the nucleotide sequence of the segment termini with the consensus 5' and 3' terminal noncoding sequences of the human group c rotavirus genome revealed characteristic 5' and 3' sequences. human group c rotavirus genome segment 11 is 613 bp long and encodes a single open reading frame of 450 nucleotides (150 amino acids) starting at nucleotide 39 a ... | 1995 | 8560785 |
| prevalence of serotypes g6 and g10 group a rotaviruses in dairy calves in quebec. | fecal samples from diarrheic and nondiarrheic dairy calves (1 to 3 weeks old) from 12 regions of quebec, collected between 1992 and 1994, were screened for group a bovine rotavirus (brv) using a combination of 2 vp6-specific monoclonal antibodies (mabs) in an enzyme-linked immunosorbent assay (elisa). the overall prevalence of brv infection was 26.4% (107/405). in diarrheic calves, brv infection reached 74.3% (55/74), but only 15.7% (52/331) in nondiarrheic calves. brv-positive samples were sero ... | 1995 | 8521359 |
| enteric adenovirus infection among infants with diarrhea in rural bangladesh. | a total of 4,409 stool specimens from infants less than 5 years of age seeking treatment for diarrhea in matlab, bangladesh, were tested for the presence of adenoviruses by using an enzyme immunoassay (eia). eia-positive stool samples were serotyped with monoclonal antibodies specific for adenovirus type 40 (ad40) and ad41 and group antigen, inoculated into graham g293 cells, and retested by eia. of adenovirus-positive cultures, 125 (2.8%) specimens were confirmed as enteric adenoviruses (eads), ... | 1993 | 8458940 |
| genomic concatemerization/deletion in rotaviruses: a new mechanism for generating rapid genetic change of potential epidemiological importance. | three variants of group a rotavirus with large changes in their gene 5 structures have been analyzed at the molecular level. the first of these, p9 delta 5, was obtained during plaque purification undertaken as part of the biological cloning of a field isolate of virus. the gene 5 homolog in this isolate migrated just ahead of the normal segment 6 rna, giving an estimated size of 1,300 bp. molecular cloning and sequencing of this homolog revealed it to have a single 308-bp deletion in the center ... | 1993 | 8411365 |
| detection of rotavirus serotypes g1, g2, g3, and g11 in feces of diarrheic calves by using polymerase chain reaction-derived cdna probes. | on the basis of antigenic variability in the vp7 outer capsid glycoprotein, at least 14 g serotypes exist for group a rotaviruses. serotypic diversity exists among bovine rotaviruses (brv), with serotypes g1, g6, g8, and g10 reported for cattle. although g1 and g8 rotaviruses were originally described for humans, the recent isolation of g6 and g10 rotaviruses from humans further emphasizes the serotypic similarity between human and bovine rotaviruses and the possible zoonotic potential of rotavi ... | 1993 | 8408573 |
| distinct yearly change of serotype distribution of human rotavirus in thailand as determined by elisa and pcr. | a total of 241 group a rotavirus-positive stool samples collected from diarrhoeic patients in thailand between july 1988 and june 1991 were characterized for their serotypes by enzyme-linked immunosorbent assay (elisa) using serotype-specific monoclonal antibodies and by a polymerase chain reaction (pcr). in july 1988-june 1989, serotype 1 was the most prevalent (63.4%), followed by serotype 4 (11.0%) and serotype 2 (8.5%). in july 1989-june 1990, 59.8% were serotype 1, 24.3% were serotype 2, an ... | 1993 | 8405164 |
| isolation and identification of rotaviruses as aetiological agents of neonatal diarrhoea in kids. electrophoretical characterization by page. | six strains of cytopathogenic enteric virus for the continuous cell line ma-104 were isolated from samples of the diarrhoeic stools of kids (capra hyrcus) with acute enteritis. the samples were obtained from two stock farms (rm and si) in central italy. four viruses from the rm stock were identified as rotaviruses of the antigenic group a by the elisa test performed with sa-11 antirotavirus monoclonal antibodies and the direct immunofluorescence test with igg bovine antirotavirus. electrophoreti ... | 1993 | 8396195 |
| experimental infection of specific-pathogen-free turkey poults with single and combined enterovirus and group a rotavirus. | this study was designed to investigate the effects of orally administered single and combined enterovirus and group a rotavirus in specific-pathogen-free poults of different ages. clinical signs, body-weight gain, d-xylose absorption, and morphometric analysis revealed that poults given the combined enterovirus/rotavirus inoculum were more severely affected than poults that received either inoculum alone. the poults developed a malabsorption syndrome early in the disease process, but by day 8 po ... | 1993 | 8395805 |
| gene 5 of the idir agent (group b rotavirus) encodes a protein equivalent to ns34 of group a rotavirus. | gene 5 of the idir strain of group b rotavirus was cloned, sequenced, and expressed in rbc lysates in order to identify the coding assignment. the complete nucleic acid sequence of idir gene 5 included 1306 bases and encoded a single long open reading frame of 390 amino acids with a predicted molecular weight of 44.8 kda. comparison of the predicted amino acid sequence indicated substantial identity with the ns34 protein of group a rotaviruses (gar). like gar ns34, the product deduced from idir ... | 1993 | 8395119 |
| a one-year survey of rotavirus strains from three locations in bangladesh. | group a rotavirus strains from 3 locations in bangladesh collected over one year were examined. serotypes 1-4 were found throughout in 2 locations, whereas an epidemic of rv diarrhoea due to a single rotavirus strain occurred in one location. | 1993 | 8394689 |
| [cat sera neutralize rotaviruses of serotype g3]. | group a rotaviruses play an important role for the induction of gastroenteritis. seroepidemiological studies evaluating the situation in humans have been performed previously. in this study data concerning the importance of group a rotavirus infections and the contribution of the most important serotypes are given for the domestic cat. 91% of the observed sera showed antibodies neutralizing serotype g 3. antibodies with neutralizing properties directed against other important human serotypes cou ... | 1993 | 8393401 |