Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| adenovirus-mediated thymidine kinase gene therapy induces apoptosis in human epithelial ovarian cancer cells and damages parp-1. | adenoviral (adv) gene therapy with the thymidine kinase gene (tk) under control of the rous sarcoma virus (rsv) promotor followed by the administration of acyclovir leads to replication errors in transcription and to cell death. this concept of adv-rsv-tk has been established for the treatment of ovarian cancer cells. the purpose of this investigation was to clarify whether cell death after adv-rsv-tk gene therapy and acyclovir administration is indeed due to apoptosis induction, whether the syn ... | 2009 | 19368128 |
| humoral immunity to respiratory syncytial virus in young and elderly adults. | respiratory syncytial virus (rsv) has been demonstrated to cause substantial disease in elderly and immunocompromised subjects. the relationship of serum antibody to rsv infection and the risk of infection in elderly subjects is controversial, thus we evaluated the presence of neutralizing antibodies to rsv in healthy people of different age groups and the correlation with viral protection. baseline blood samples from 197 subjects aged 20-80 years were analysed for the presence of anti-rsv antib ... | 2009 | 19366492 |
| retroviral capsid assembly: a role for the ca dimer in initiation. | in maturing retroviral virions, ca protein assembles to form a capsid shell that is essential for infectivity. the structure of the two folded domains [n-terminal domain (ntd) and c-terminal domain (ctd)] of ca is highly conserved among various retroviruses, and the capsid assembly pathway, although poorly understood, is thought to be conserved as well. in vitro assembly reactions with purified ca proteins of the rous sarcoma virus (rsv) were used to define factors that influence the kinetics of ... | 2009 | 19361521 |
| [effects of san'ao decoction and its analogous prescriptions on airway inflammation in mice with respiratory syncytial virus- and ovalbumin-induced asthma]. | to evaluate the effects of san'ao decoction (sad) and its analogous prescriptions (aps), compounds of traditional chinese herbal medicine for asthma, on airway inflammation in mice with respiratory syncytial virus (rsv)- and ovalbumin (ova)-induced asthma. | 2009 | 19361366 |
| human metapneumovirus establishes persistent infection in the lungs of mice and is reactivated by glucocorticoid treatment. | human metapneumovirus (hmpv) has been identified as a worldwide agent of serious upper and lower respiratory tract infections in infants and young children. hmpv is second only to respiratory syncytial virus (rsv) as a leading cause of bronchiolitis, and, like rsv, consists of two major genotypes that cocirculate and vary among communities year to year. children who have experienced acute hmpv infection may develop sequelae of wheezing and asthma; however, the features contributing to this patho ... | 2009 | 19357175 |
| systemic cytokine response profiles associated with respiratory virus-induced acute otitis media. | acute otitis media (aom) results from a complex interplay between the infectious agents and host immune responses. cytokines play a major role in the pathogenesis of aom, but there are few studies on the systemic cytokine response during aom. | 2009 | 19352211 |
| enhanced delivery and expression of a nanoencapsulated dna vaccine vector for respiratory syncytial virus. | this study evaluated the efficiency of chitosan-encapsulated dna-based respiratory syncytial virus (rsv) vaccine. antigenic regions of rsv f, m2, and g genes were cloned into the human cytomegalovirus promoter-based constitutive expression vector, resulting in a dna vaccine vector named dr-fm2g. this vector was used to formulate dna-chitosan nanoparticles (dcnps) using a complex coacervation process that yielded an encapsulation efficiency of 94.7%. the dcnp sizes ranged from 80 to 150 nm with u ... | 2009 | 19341819 |
| [use of multiplex pcr method with real-time detection for differential diagnosis of respiratory viral infections]. | multiplex real-time polymerase chain reaction test-system with fluorescent detection (rt-pcr) for simultaneous identification of main agents of acute respiratory viral infections: influenza a (iav) and b viruses (ibv), parainfluenza viruses types 1, 2, 3, 4 (piv 1 - 4), adenoviruses (adv), respiratory syncitial virus (rsv), rhinoviruses (rv) and enteroviruses (ev), in presence internal positive control (ipc) represented by vaccine strain of rubella virus ra 27/3. using multiplex rt-pcr method, r ... | 2009 | 19338239 |
| motavizumab, a second-generation humanized mab for the prevention of respiratory syncytial virus infection in high-risk populations. | evolved from palivizumab, motavizumab is a second-generation humanized mab that is in development by medimmune for the prevention of respiratory syncytial virus (rsv) infection in high-risk populations; the drug is also under investigation for the same indication by abbott laboratories. motavizumab targets a highly conserved epitope in the a antigenic site of the rsv fusion (f) protein, which is important in the invasion of rsv from cell to cell. motavizumab, which differs from palivizumab by ju ... | 2009 | 19330726 |
| the host response and molecular pathogenesis associated with respiratory syncytial virus infection. | since the isolation of respiratory syncytial virus (rsv) in 1956, its significance as an important human pathogen in infants, the elderly and the immunocompromised has been established. many important mechanisms contributing to rsv infection, replication and disease pathogenesis have been uncovered; however, there is still insufficient knowledge in these and related areas, which must be addressed to facilitate the development of safe and effective vaccines and therapeutic treatments. a better un ... | 2009 | 19327115 |
| bronchiolitis: from empiricism to scientific evidence. | bronchiolitis is the most common viral infection of the lower respiratory tract in infants in their first year of life, with an incidence peak between 3 and 9 months of age. the clinical profile of bronchiolitis results from the inflammatory obstruction of the small airways. the etiological agent involved is respiratory syncytial virus (rsv) in more than 50% of cases. the first international guidelines on the management of children with bronchiolitis have recently been published. the first was p ... | 2009 | 19322125 |
| an outbreak of human metapneumovirus infection in hospitalized psychiatric adult patients in taiwan. | human metapneumovirus (hmpv) is a paramyxovirus that is associated with respiratory tract infection (rti) mostly in children, but these outbreaks have rarely been reported in adults. we encountered an outbreak of this disease involving 10 adults in a psychiatric ward in eastern taiwan. the nasopharyngeal swab specimens from 13 patients with symptoms of rti were obtained and analyzed. the rt-pcr tests were negative to influenza virus a/b, adenovirus, rsv, parainfluenza virus, coronavirus, nipah v ... | 2009 | 19308801 |
| surfactant protein c-deficient mice are susceptible to respiratory syncytial virus infection. | patients with mutations in the pulmonary surfactant protein c (sp-c) gene develop interstitial lung disease and pulmonary exacerbations associated with viral infections including respiratory syncytial virus (rsv). pulmonary infection with rsv caused more severe interstitial thickening, air space consolidation, and goblet cell hyperplasia in sp-c-deficient (sftpc(-/-)) mice compared with sp-c replete mice. the rsv-induced pathology resolved more slowly in sftpc(-/-) mice with lung inflammation pe ... | 2009 | 19304906 |
| 4-methoxycinnamaldehyde inhibited human respiratory syncytial virus in a human larynx carcinoma cell line. | 4-methoxycinnamaldehyde, an active constituent of agastache rugosa, was examined for its cytoprotective activity against rsv by xtt method in human larynx carcinoma cell line. 4-methoxycinnamaldehyde could effectively inhibit cytopathic effect of rsv (p<0.0001) with an estimated ic(50) of 0.055microg/ml and a selectivity index (si) of 898.2. 4-methoxycinnamaldehyde (0.03microg/ml) could inhibit viral entrance by interfering viral attachment (ic(50) of 0.06microg/ml; p<0.0001) and internalization ... | 2009 | 19303275 |
| nasopharyngeal aspirate cytokine levels 1 yr after severe respiratory syncytial virus infection. | respiratory syncytial virus (rsv) infection is an important cause of recurrent wheezing in infants. nevertheless, the link between rsv infection and wheezing has yet to be elucidated at the molecular level. here, we present a preliminary study on the evolution of the immune response in the respiratory tract at long-term after rsv infection. twenty-seven immune mediators were profiled in nasopharyngeal aspirates (npas) obtained from 20 children hospitalized due to a severe infection by rsv at dis ... | 2009 | 19302175 |
| treating respiratory viral diseases with chemically modified, second generation intranasal sirnas. | chemically synthesized short interfering rna (sirna) of pre-determined sequence has ushered a new era in the application of rna interference (rnai) against viral genes. we have paid particular attention to respiratory viruses that wreak heavy morbidity and mortality worldwide. the clinically significant ones include respiratory syncytial virus (rsv), parainfluenza virus (piv) and influenza virus. as the infection by these viruses is clinically restricted to the respiratory tissues, mainly the lu ... | 2009 | 19301655 |
| the respiratory syncytial virus matrix protein possesses a crm1-mediated nuclear export mechanism. | the respiratory syncytial virus (rsv) matrix (m) protein is localized in the nucleus of infected cells early in infection but is mostly cytoplasmic late in infection. we have previously shown that m localizes in the nucleus through the action of the importin beta1 nuclear import receptor. here, we establish for the first time that m's ability to shuttle to the cytoplasm is due to the action of the nuclear export receptor crm1, as shown in infected cells, and in cells transfected to express green ... | 2009 | 19297465 |
| [viral pathogens of acute lower respiratory tract infection in hospitalized children from east guangdong of china]. | to investigate the viral pathogens of acute lower respiratory tract infection (alrti) in hospitalized children from east guangdong province of china and the relationship of the pathogens with age and seasons. | 2009 | 19292960 |
| incidence and clinical characteristics of the infection by the respiratory syncytial virus in children admitted in santa casa de são paulo hospital. | the purpose of this study was to identify the rate of infections due to rsv and other viruses in children. in addition we have analyzed demographic data and clinical characteristics of the rsv-positive patients comparing with patients infected by other respiratory viruses. we also described the seasonality of the rsv occurrence in a hospital in são paulo. children below 5 years old admitted in santa casa de são paulo hospital between february 2005 and september 2006 due to acute respiratory infe ... | 2008 | 19287833 |
| transmission of surfactant protein variants and haplotypes in children hospitalized with respiratory syncytial virus. | severity of lung injury with respiratory syncytial virus (rsv) infection is variable and may be related to genetic variations. this preliminary report describes a prospective, family-based association study of children hospitalized secondary to rsv, aimed to determine whether intragenic and other haplotypes of surfactant proteins (sp)-a and sp-d are transmitted disproportionately from parents to offspring with rsv disease. genomic dna was genotyped for several sp-a and sp-d single nucleotide pol ... | 2009 | 19287351 |
| adenovirus-mediated thymidine kinase gene therapy and coxsackie adenovirus receptor expression in ovarian cancer cells. | coxsackie adenovirus receptor (car) expression is the main mechanism of adenovirus entry into target cells. it is unclear whether car expression itself is influenced by transduction with the adenovirus-rous sarcoma virus-thymidine kinase (adv-rsv-tk) gene therapy construct or by the subsequent intracellular accumulation of the tk gene product. antibody generation and characterization, immunocytochemistry, western blotting and 3-(4,5-dimethylthiazol)-2,5-diphenyl tetrazolium bromide (mtt) assay w ... | 2008 | 19287072 |
| exploring the association between severe respiratory syncytial virus infection and asthma: a registry-based twin study. | severe respiratory syncytial virus (rsv) infection is associated with asthma but the nature of this association is imperfectly understood. | 2009 | 19286626 |
| etiology of viral pneumopathies in patients in intensive care unit under mechanical ventilation. | the objective of this work was to define the etiology of viral pneumopathies at the patients from reanimation section being under mechanical ventilation, making reference to viruses with respiratory tropism, and also to chlamydia pneumoniae and mycoplasma pneumoniae. the subjects were 36 patients hospitalized into service of medical reanimation from chu caen and who needed mechanical ventilation more than 48 hours. the samples from the patients were mostly nasal aspirate, 1 bronchial aspirate an ... | 2008 | 19284161 |
| innate immune signals modulate antiviral and polyreactive antibody responses during severe respiratory syncytial virus infection. | antiviral antibody production during respiratory syncytial virus (rsv) infection in infants is poorly understood. to characterize local b lymphocyte responses, lung tissue and secretions from infants with rsv bronchiolitis were analyzed for innate b cell-stimulating factors and antiviral antibodies. in lung tissues of infants with fatal rsv bronchiolitis, cd20(+) lymphocytes and igm-positive, igg-positive, and iga-positive plasma cells were prominent but cd4(+) t lymphocytes were not. type i int ... | 2009 | 19278337 |
| immune dysregulation in severe influenza. | among previously healthy children with severe influenza, the mechanisms leading to increased pathology are not understood. we hypothesized that children with severe influenza would have high levels of circulating cytokines. to examine this, we recruited patients with severe influenza and examined plasma cytokine levels as well as the ability of peripheral blood cells to respond to stimuli. ten patients with severe influenza were enrolled during the 2005-2007 influenza seasons. we evaluated plasm ... | 2009 | 19276177 |
| characterization of respiratory syncytial virus m- and m2-specific cd4 t cells in a murine model. | cd4 t cells have been shown to play an important role in the immunity and immunopathogenesis of respiratory syncytial virus (rsv) infection. we identified two novel cd4 t-cell epitopes in the rsv m and m2 proteins with core sequences m(213-223) (fkyikpqsqfi) and m2(27-37) (yfewpphallv). peptides containing the epitopes stimulated rsv-specific cd4 t cells to produce gamma interferon (ifn-gamma), interleukin 2 (il-2), and other th1- and th2-type cytokines in an i-a(b)-restricted pattern. construct ... | 2009 | 19264776 |
| genetic diversity and population structure of rice stripe virus in china. | rice stripe virus (rsv) is one of the most economically important pathogens of rice and is repeatedly epidemic in china, japan and korea. the most recent outbreak of rsv in eastern china in 2000 caused significant losses and raised serious concerns. in this paper, we provide a genotyping profile of rsv field isolates and describe the population structure of rsv in china, based on the nucleotide sequences of isolates collected from different geographical regions during 1997-2004. rsv isolates cou ... | 2009 | 19264655 |
| treatment with respiratory syncytial virus g glycoprotein monoclonal antibody or f(ab')2 components mediates reduced pulmonary inflammation in mice. | therapeutic treatment with a non-neutralizing monoclonal antibody (mab) (131-2g) specific to respiratory syncytial virus (rsv) g glycoprotein mediates virus clearance and decreases leukocyte trafficking and interferon gamma (ifn-gamma) production in the lungs of rsv-infected mice. its f(ab')(2) component only mediates decreased leukocyte trafficking and ifn-gamma production without reducing virus replication. thus, this mab has two independent actions that could facilitate treatment and/or preve ... | 2009 | 19264600 |
| cytomegalovirus infection and wheezing in infants. | bronchial asthma-like symptoms such as wheezing are commonly associated with respiratory tract infection including respiratory syncytial virus (rsv) infection in infants. no study on the association of wheezing with cytomegalovirus (cmv) infection in infancy has been reported, although cmv infection has been observed to play some role in prolonged and intractable wheezing in limited cases. | 2008 | 19261114 |
| safety, tolerability, pharmacokinetics, and immunogenicity of motavizumab, a humanized, enhanced-potency monoclonal antibody for the prevention of respiratory syncytial virus infection in at-risk children. | : respiratory syncytial virus (rsv) is a major cause of lower respiratory tract infection in young children. motavizumab is an investigational humanized monoclonal antibody for rsv prophylaxis. | 2009 | 19258920 |
| epidemiology of respiratory syncytial virus in various regions within north carolina during multiple seasons. | the centers for disease control and prevention (cdc) monitors the occurrence of respiratory syncytial virus (rsv) in the united states and has historically reported on activity at the regional level. prior to the 2007-2008 rsvseason, the cdc did not report seasonal rsv data for cities within north carolina or for the state. the purpose of the present study is to characterize rsv seasonal activity within north carolina and to determine the appropriate months in which at-risk children should recei ... | 2008 | 19256181 |
| surface features of a mononegavirales matrix protein indicate sites of membrane interaction. | the matrix protein (m) of respiratory syncytial virus (rsv), the prototype viral member of the pneumovirinae (family paramyxoviridae, order mononegavirales), has been crystallized and the structure determined to a resolution of 1.6 a. the structure comprises 2 compact beta-rich domains connected by a relatively unstructured linker region. due to the high degree of side-chain order in the structure, an extensive contiguous area of positive surface charge covering approximately 600 a(2) can be res ... | 2009 | 19251668 |
| characterization and subcellular localization of an rna silencing suppressor encoded by rice stripe tenuivirus. | rice stripe virus (rsv) is a single-stranded (ss) rna virus belonging to the genus tenuivirus. rsv is present in many east asian countries and causes severe diseases in rice fields, especially in china. in this study, we analyzed six proteins encoded by the virus for their abilities to suppress rna silencing in plant using a green fluorescent protein (gfp)-based transient expression assay. our results indicate that ns3 encoded by rsv rna3, but not other five rsv encoded proteins, can strongly su ... | 2009 | 19251298 |
| in very young infants severity of acute bronchiolitis depends on carried viruses. | rt amplification reaction has revealed that various single viruses or viral co-infections caused acute bronchiolitis in infants, and rv appeared to have a growing involvement in early respiratory diseases. because remaining controversial, the objective was to determine prospectively the respective role of rsv, rv, hmpv and co-infections on the severity of acute bronchiolitis in very young infants. | 2009 | 19240806 |
| human metapneumovirus infections--biannual epidemics and clinical findings in children in the region of basel, switzerland. | human metapneumovirus (hmpv) epidemics vary in time and severity. we report findings for pcr for hmpv and respiratory syncytial virus (rsv) performed on nasopharyngeal aspirates (npa) of hospitalized and outpatient children with respiratory tract infections between october 2004 and april 2008. a total of 3,934 npas were tested for hmpv and 3,859 for rsv. of these, 198 (5%) were hmpv positive and 869 (23%) were rsv-positive. median age was 17 months and 9 months for hmpv and rsv, respectively. fi ... | 2009 | 19238433 |
| epidemiological and clinical study of viral respiratory tract infections in children from italy. | impact of recently discovered viruses on epidemiology of acute respiratory tract infections (arti) is still unclear. we studied the impact of recently discovered human metapneumovirus (hmpv), human bocavirus (hbov), and new coronaviruses (hcov-nl63 and hku1) on the global epidemiology of arti. from october 2006 to april 2007, 237 pediatric patients affected by arti were enrolled in our study. specimens were tested for respiratory viruses by polymerase chain reaction. one hundred twenty-four out ... | 2009 | 19235872 |
| subversion of pulmonary dendritic cell function by paramyxovirus infections. | lower respiratory tract infections caused by the paramyxoviruses human metapneumovirus (hmpv) and respiratory syncytial virus (rsv) are characterized by short-lasting virus-specific immunity and often long-term airway morbidity, both of which may be the result of alterations in the ag-presenting function of the lung which follow these infections. in this study, we investigated whether hmpv and rsv experimental infections alter the phenotype and function of dendritic cell (dc) subsets that are re ... | 2009 | 19234204 |
| fas ligand is required for the development of respiratory syncytial virus vaccine-enhanced disease. | children immunized with a formalin-inactivated respiratory syncytial virus (rsv) vaccine experienced enhanced disease and exhibited pulmonary eosinophilia upon natural rsv infection. balb/c mice immunized with either formalin-inactivated rsv or a recombinant vaccinia virus (vacv) expressing the rsv attachment (g) protein develop extensive pulmonary eosinophilia after rsv challenge that mimics the eosinophilic response observed in the children during the 1960s vaccine trials. fas ligand (fasl) is ... | 2009 | 19234198 |
| murine host responses to respiratory syncytial virus (rsv) following intranasal administration of a protollin-adjuvanted, epitope-enhanced recombinant g protein vaccine. | immunization of mice with the g protein of respiratory syncytial virus (rsv) characteristically induces an immune response that is partially protective, but which can prime for pulmonary eosinophilia. we have shown previously that the n191a mutation in a recombinant rsv g protein fragment is associated with reduced pulmonary eosinophilic infiltration when administered with alum subcutaneously in balb/c mice followed by rsv challenge. we hypothesize that the performance of this "epitope enhanced" ... | 2009 | 19233722 |
| intranasal immunization with a replication-deficient adenoviral vector expressing the fusion glycoprotein of respiratory syncytial virus elicits protective immunity in balb/c mice. | human respiratory syncytial virus (rsv) is a serious pediatric pathogen of the lower respiratory tract worldwide. there is currently no clinically approved vaccine against rsv infection. recently, it has been shown that a replication-deficient first generation adenoviral vector (fgad), which encodes modified rsv attachment glycoprotein (g), elicits long-term protective immunity against rsv infection in mice. the major problem in developing such a vaccine is that g protein lacks mhc-i-restricted ... | 2009 | 19233131 |
| respiratory syncytial virus: from primary care to critical care. | respiratory syncytial virus (rsv) is a common disease in pediatrics. certain subpopulations of children are at greatest risk for serious disease. however, previously healthy children also may become critically ill. in the clinic or the intensive care unit, children with rsv pose the challenge of how to treat a disease when evidence to support therapeutic options is severely limited. prophylaxis is an option for certain children, although many do not qualify. rsv has been implicated in continued ... | 2009 | 19232926 |
| recognizing the importance of respiratory syncytial virus in chronic obstructive pulmonary disease. | acute exacerbations of chronic obstructive pulmonary disease (copd) are responsible for a large proportion of the health care dollar expenditure, morbidity, and mortality related to copd. respiratory infections are the most common cause of acute exacerbations, but recent evidence indicates that the importance of respiratory syncytial virus (rsv) infection in copd is under-appreciated. improved detection of rsv using techniques based on the polymerase chain reaction accounts for much of the incre ... | 2009 | 19229710 |
| foreign glycoproteins can be actively recruited to virus assembly sites during pseudotyping. | retroviruses like human immunodeficiency virus type 1 (hiv-1), as well as many other enveloped viruses, can efficiently produce infectious virus in the absence of their own surface glycoprotein if a suitable glycoprotein from a foreign virus is expressed in the same cell. this process of complementation, known as pseudotyping, often can occur even when the glycoprotein is from an unrelated virus. although pseudotyping is widely used for engineering chimeric viruses, it has remained unknown wheth ... | 2009 | 19224995 |
| micronutrient concentrations in respiratory syncytial virus and human metapneumovirus in yemeni children. | acute respiratory infections (ari) cause significant childhood mortality. nutritional homeostasis, particularly micronutrient levels, is important in modulating response to infection. more information is required regarding micronutrient levels in ari viral infections, especially newly identified viruses such as human metapneumovirus (hmpv). | 2009 | 19222932 |
| school age outcome of hospitalisation with respiratory syncytial virus infection of prematurely born infants. | hospitalisation due to respiratory syncytial virus (rsv) infection in the first 2 years after birth has been associated with increased healthcare utilisation and associated costs up to 5 years of age in children born prematurely at less than 32 weeks of gestation who developed bronchopulmonary dysplasia (bpd). a study was undertaken to determine whether hospitalisation due to rsv infection in the first 2 years was associated with increased morbidity and lung function abnormalities in such childr ... | 2009 | 19213770 |
| a chimeric a2 strain of respiratory syncytial virus (rsv) with the fusion protein of rsv strain line 19 exhibits enhanced viral load, mucus, and airway dysfunction. | respiratory syncytial virus (rsv) is the leading cause of respiratory failure and viral death in infants. abundant airway mucus contributes to airway obstruction in rsv disease. interleukin-13 (il-13) is a mediator of pulmonary mucus secretion. it has been shown that infection of balb/c mice with the rsv line 19 strain but not with the rsv a2 laboratory strain results in lung il-13 and mucus expression. here, we sequenced the rsv line 19 genome and compared it to the commonly used a2 and long st ... | 2009 | 19211758 |
| infant exposure to fine particulate matter and traffic and risk of hospitalization for rsv bronchiolitis in a region with lower ambient air pollution. | few studies investigate the impact of air pollution on the leading cause of infant morbidity, acute bronchiolitis. we investigated the influence of pm(2.5) and other metrics of traffic-derived air pollution exposure using a matched case-control dataset derived from 1997 to 2003 birth and infant hospitalization records from the puget sound region, washington state. mean daily pm(2.5) exposure for 7, 30, 60 and lifetime days before case bronchiolitis hospitalization date were derived from communit ... | 2009 | 19211100 |
| [respiratory syncytial virus: as an etiological agent of respiratory tract infection in children and adults]. | respiratory syncytial virus is the most important cause of respiratory tract infection in infants, young children and immunocompromised adults. rsv disease spectrum includes a wide array of respiratory symptoms, from rhinitis and otits media to pneumonia and bronchiolitis. studies have implicated severe rsv infection early in life as a risk factor for subsequent development of reactive airway disease. rsv-infected patients indicated increased levels of th2 cytokines and ige in the patients sera, ... | 2008 | 19209739 |
| optimization strategy to minimize wastage of palivizumab during the 2008 rsv season in são paulo, brazil. | palivizumab is currently recommended to high-risk children as a prophylaxis for respiratory syncytial virus (rsv) infection. however, it is still very expensive for developing countries like brazil. herein, we describe our strategy to minimize wastage of palivizumab during the 2008 rsv season. appointments were fixed for 304 children on 2 days of the week, so that a mean of 19.9 children received palivizumab per day. that allowed remaining volumes of palivizumab vials to be pooled and used for o ... | 2009 | 19203985 |
| human piv-2 recombinant sendai virus (rsev) elicits durable immunity and combines with two additional rsevs to protect against hpiv-1, hpiv-2, hpiv-3, and rsv. | the human parainfluenza viruses (hpivs) and respiratory syncytial viruses (rsvs) are the leading causes of hospitalizations due to respiratory viral disease in infants and young children, but no vaccines are yet available. here we describe the use of recombinant sendai viruses (rsevs) as candidate vaccine vectors for these respiratory viruses in a cotton rat model. two new sendai virus (sev)-based hpiv-2 vaccine constructs were generated by inserting the fusion (f) gene or the hemagglutinin-neur ... | 2009 | 19200447 |
| the burden of respiratory syncytial virus infection in young children. | the primary role of respiratory syncytial virus (rsv) in causing infant hospitalizations is well recognized, but the total burden of rsv infection among young children remains poorly defined. | 2009 | 19196675 |
| mast cell degranulation is induced by a549 airway epithelial cell infected with respiratory syncytial virus. | respiratory syncytial virus (rsv), a major causative agent of respiratory tract infections, influences allergic diseases. mast cells, important effector cells in allergic disease, also express chemokine (c-x(3)-c motif) receptor 1 (cx(3)cr1). the rsv attachment glycoprotein (g protein) is structurally similar to cx(3)c ligand 1 (cx(3)cl1), the cx(3)cr1 ligand, suggesting that rsv directly interacts with and affects mast cell function, including degranulation. in this paper, the effect of rsv inf ... | 2009 | 19195674 |
| human respiratory syncytial virus nonstructural protein ns2 antagonizes the activation of beta interferon transcription by interacting with rig-i. | a wide variety of rna viruses have been shown to produce proteins that inhibit interferon (ifn) production and signaling. for human respiratory syncytial virus (rsv), the nonstructural ns1 and ns2 proteins have been shown to block ifn signaling by causing the proteasomal degradation of stat2. in addition, recombinant rsvs lacking either ns1 or ns2 induce more ifn production than wild-type (wt) rsv in infected cells. however, the mechanisms by which the ns proteins perform this function are unkno ... | 2009 | 19193793 |
| establishing a surveillance network for severe lower respiratory tract infections in korean infants and young children. | to reduce morbidity and mortality through integrated case management, a pilot study to detect respiratory viruses in patients with acute lower respiratory infections (alris) was designed as part of a nationwide surveillance for this disease in korea. the study population consisted of hospitalized patients under the age of 5 years with bronchiolitis, pneumonia, croup, or acute respiratory distress syndrome. a prospective 6-month study was performed. two hundred and ninety-seven nasopharyngeal sec ... | 2009 | 19190941 |
| gene profiling studies in the neonatal ovine lung show enhancing effects of vegf on the immune response. | preterm and young neonates have an increased predisposition to respiratory distress syndrome (rds) associated with an immature development of lung surfactant. glucocorticoids (gcs) are the major immunomodulatory agents used to increase lung development and reduce the mortality and morbidity of preterm infants with rds. however, their safety remains uncertain, and the precise mechanisms by which they improve lung function are unclear. in previous studies, we found that vascular endothelial growth ... | 2009 | 19189846 |
| respiratory syncytial virus outbreak in a long-term care facility detected using reverse transcriptase polymerase chain reaction: an argument for real-time detection methods. | to report an outbreak of respiratory synctyial virus (rsv) in a long-term care facility (ltcf) during ongoing routine respiratory illness surveillance. | 2009 | 19187415 |
| comparison of cytokine responses in nasopharyngeal aspirates from children with viral lower respiratory tract infections. | to determine whether nasopharyngeal aspirates (npas) cytokine response is different according to the causative viruses in children with lower respiratory tract infections (lrti). | 2009 | 19183120 |
| evidence that selective changes in the lipid composition of raft-membranes occur during respiratory syncytial virus infection. | we examined the structure of lipid-raft membranes in respiratory syncytial virus infected cells. cholesterol depletion studies using methyl-beta-cyclodextrin suggested that membrane cholesterol was required for virus filament formation, but not inclusion bodies. in addition, virus filament formation coincided with elevated 3-hydroxy-3-methylglutaryl-coenzyme a reductase expression, suggesting an increase in requirement for endogenous cholesterol synthesis during virus assembly. lipid raft membra ... | 2009 | 19178924 |
| delayed exposure to infections and childhood lymphomas: a case-control study. | delayed exposure to common infections during childhood, have been implied to cause strong immunological response to a single infectious agent that eventually triggers leukemogenesis. the aim of the present study was to investigate whether decreased exposure to infections, as reflected in a more seronegative spectrum to several common infectious agents, is associated with increased risk for the development of childhood lymphomas. | 2009 | 19169895 |
| an unusual respiratory syncytial virus nosocomial outbreak in an adult psychiatry ward. | herein we report our experience in containing an outbreak of nosocomial respiratory syncytial virus (rsv) infection in a psychiatric ward in central taiwan during a non-widespread rsv seasonal occurrence. a total of 8 patients and 4 healthcare workers in the psychiatric ward developed febrile illness or upper respiratory tract infection symptoms between august 23 and 29, 2005. rsv was identified by either viral culture or reverse transcriptase-polymerase chain reaction (rt-pcr) assay. rsv was is ... | 2009 | 19168963 |
| integrin alphavbeta1 promotes infection by human metapneumovirus. | human metapneumovirus (hmpv) is a recently described paramyxovirus that causes lower respiratory infections in children and adults worldwide. the hmpv fusion (f) protein is a membrane-anchored glycoprotein and major protective antigen. all hmpv f protein sequences determined to date contain an arg-gly-asp (rgd) sequence, suggesting that f engages rgd-binding integrins to mediate cell entry. the divalent cation chelator edta, which disrupts heterodimeric integrin interactions, inhibits infectivit ... | 2009 | 19164533 |
| ability of low-molecular-weight heparin to alleviate proteinuria by inhibiting respiratory syncytial virus infection. | low-molecular-weight heparin (lmwh) is a negatively charged glycoprotein and has a very similar structure to that of cell surface heparin sulfate (hs). thus, lmwh, an analog of hs, may inhibit positively charged respiratory syncytial virus (rsv) infection through cooperative electrostatic association. | 2008 | 19161362 |
| broad spectrum antiviral fractions from the lichen ramalina farinacea (l.) ach. | an ethylacetate-soluble fraction (et4) from the lichen ramalina farinacea has previously been shown to inhibit the infectivity of lentiviral and adenoviral vectors, as well as wild-type hiv-1. we now determined the antiviral activity of et4 against other wild-type viruses, including the herpes simplex virus type 1 (hsv-1) and the respiratory syncytial virus (rsv). | 2009 | 19155620 |
| regulatory t cells promote early influx of cd8+ t cells in the lungs of respiratory syncytial virus-infected mice and diminish immunodominance disparities. | in addition to regulating autoimmunity and antitumor immunity, cd4(+) cd25(+) foxp3(+) natural regulatory t (treg) cells are global regulators of adaptive immune responses. depletion of these cells with the anti-cd25 antibody pc61 prior to primary respiratory syncytial virus (rsv) infection was partial but had several effects on the rsv-specific cd8(+) response in a hybrid mouse model. mediastinal lymph node and spleen epitope-specific cd8(+) t-cell responses were enhanced in treg-cell-depleted ... | 2009 | 19153229 |
| respiratory syncytial virus induces oxidative stress by modulating antioxidant enzymes. | oxidative stress plays an important role in the pathogenesis of lung inflammation. respiratory syncytial virus (rsv) infection induces reactive oxygen species (ros) production in vitro and oxidative injury in lungs in vivo; however, the mechanism of rsv-induced cellular oxidative stress has not been investigated. therefore, we determined whether rsv infection of airway epithelial cells modified the expression and/or activities of antioxidant enzymes (aoe). a549 cells, a human alveolar type ii-li ... | 2009 | 19151318 |
| seasonal variation of maternally derived respiratory syncytial virus antibodies and association with infant hospitalizations for respiratory syncytial virus. | this study used 459 prospectively sampled cord blood samples to examine the association between maternally derived respiratory syncytial virus (rsv)-neutralizing antibodies and the rsv hospitalization season in denmark. we found a clear temporal association and suggest that rsv-neutralizing antibody level plays a role in the rsv seasonal pattern. | 2009 | 19150677 |
| [association of inf-gamma/a+874t gene polymorphisms with respiratory syncytial virus bronchiolitis]. | a deficient interferon-gamma (ifn-gamma) response has been involved in the pathogenesis of severe respiratory syncytial virus (rsv) infection. gene polymorphisms in ifn-gamma/a+874t have been associated with the susceptibility to asthma and might be related to disease severity of rsv infection. this study investigated the single nucleotide polymorphisms (snps) of ifn-gamma/a+874t in han children in wenzhou area and to explore the correlation between gene polymorphisms of ifn-gamma/a+874t and the ... | 2009 | 19149916 |
| progress towards recombinant anti-infective antibodies. | the global market for monoclonal antibody therapeutics reached a total of $11.2 billion in 2004, with an impressive 42% growth rate over the previous five years and is expected to reach approximately $34 billion by 2010. coupled with this growth are stream-lined product development, production scale-up and regulatory approval processes for the highly conserved antibody structure. while only one of the 21 current fda-approved antibodies, and one of the 38 products in advanced clinical trials targ ... | 2009 | 19149692 |
| risk of respiratory syncytial virus in survivors with severe congenital diaphragmatic hernia. | during the follow-up period in surviving patients with severe congenital diaphragmatic hernia (cdh), respiratory complications, such as recurrence of cdh or chronic lung disease, have been reported to occur as a late complication. although some risk factors for deterioration of respiratory condition have been reported in cdh, the risk of respiratory syncytial virus (rsv) in postoperative cdh patients has not as yet been reported upon. | 2008 | 19143967 |
| lower levels of plasmacytoid dendritic cells in peripheral blood are associated with a diagnosis of asthma 6 yr after severe respiratory syncytial virus bronchiolitis. | plasmacytoid dendritic cells (pdc) play a crucial role in antiviral immunity and promoting th1 polarization, possibly protecting against development of allergic disease. examination of the relationship between peripheral blood plasmacytoid dc levels and manifestations of asthma and atopy early in life. we have isolated peripheral blood mononuclear cells (pbmc) from 73 children (mean age +/- sd: 6.6 +/- 0.5 yr old) participating in the rsv bronchiolitis in early life (rbel) study. flow cytometry ... | 2009 | 19140903 |
| efficacy of oral ribavirin in lung transplant patients with respiratory syncytial virus lower respiratory tract infection. | respiratory syncytial virus (rsv) can cause severe lower respiratory tract infection (lri) and is a risk factor for the development of bronchiolitis obliterans syndrome (bos) after lung transplantation (ltx). currently, the most widely used therapy for rsv is inhaled ribavirin. however, this therapy is costly and cumbersome. we investigated the utility of using oral ribavirin for the treatment of rsv infection after ltx. | 2009 | 19134533 |
| cigarette smoke alters respiratory syncytial virus-induced apoptosis and replication. | individuals exposed to cigarette smoke have a greater number and severity of viral infections, including respiratory syncytial virus (rsv) infections, than do nonsmokers, but the cellular mechanism is unknown. our objective was to determine the mechanism by which cigarette smoke augments viral infection. we hypothesize that cigarette smoke causes necrosis and prevents virus-induced cellular apoptosis, and that this is associated with increased inflammation and viral replication. primary airway e ... | 2009 | 19131644 |
| genetic association study for rsv bronchiolitis in infancy at the 5q31 cytokine cluster. | the pathophysiological basis of severe respiratory syncytial virus (rsv) bronchiolitis in infancy is poorly understood and has hindered vaccine development. studies implicate the cell-mediated immune response in the pathogenesis of the disease. a recent twin study estimated a heritable contribution of 22% to rsv bronchiolitis. genetic epidemiology provides a new approach to identifying important immune determinants of disease severity. | 2009 | 19131452 |
| respiratory syncytial virus inhibits lung epithelial na+ channels by up-regulating inducible nitric-oxide synthase. | respiratory syncytial virus (rsv) infection has been shown to reduce na+-driven alveolar fluid clearance in balb/c mice in vivo. to investigate the cellular mechanisms by which rsv inhibits amiloride-sensitive epithelial na+ channels (enac), the main pathways through which na+ ions enter lung epithelial cells, we infected human clara-like lung (h441) cells with rsv that expresses green fluorescent protein (rra2). 3-6 days later patch clamp recordings showed that infected cells (i.e. cells expres ... | 2009 | 19131335 |
| the causal direction in the association between respiratory syncytial virus hospitalization and asthma. | earlier studies have reported an increased risk of asthma after respiratory syncytial virus (rsv) hospitalization. other studies found that asthmatic disposition and propensity to wheeze increase the risk of rsv hospitalization. | 2009 | 19130934 |
| pc4, a putative movement protein of rice stripe virus, interacts with a type i dnaj protein and a small hsp of rice. | rice stripe virus (rsv) infects rice and causes great yield reduction in some asian countries. in this study, rice cdna library was screened by a gal4-based yeast two-hybrid system using pc4, a putative movement protein of rsv, as the bait. a number of positive colonies were identified and sequence analysis revealed that they might correspond to ten independent proteins. two of them were selected and further characterized. the two proteins were a j protein and a small hsp, respectively. interact ... | 2009 | 19130198 |
| viral pathogens of acute lower respiratory tract infection in china. | to document the viral etiology of acute lower respiratory tract infection (alris) in chinese children. | 2008 | 19129564 |
| comparison of viral isolation and multiplex real-time reverse transcription-pcr for confirmation of respiratory syncytial virus and influenza virus detection by antigen immunoassays. | we evaluated the prodesse proflu-1 real-time reverse transcription-pcr multiplex assay with the smartcycler instrument for the detection of human respiratory syncytial virus (rsv) and influenza a and b viruses in comparison to conventional cell culture and antigen immunoassays with the bd directigen a+b and binax now rsv assays over two successive respiratory virus seasons. ninety-two percent of the 361 specimens tested were nasopharyngeal aspirates obtained from individual patients, of which 11 ... | 2009 | 19129410 |
| [viral respiratory tract infections in the first six months of life]. | respiratory syncytial virus and influenza virus infections are known causes of hospital admission in infants. it is less well known the pattern of virus infections in infants under 6 months of age in the outpatient setting. | 2008 | 19128739 |
| temperature affects expression of symptoms induced by soybean mosaic virus in homozygous and heterozygous plants. | seven strains (g1 to g7) of soybean mosaic virus (smv) and 3 resistance loci (rsv1, rsv3, and rsv4) have been identified in soybean. the interaction of smv strains and host resistance genes results in resistant (symptomless), susceptible (mosaic), or necrotic (leaf and stem necrosis) reactions. the necrotic reaction may be gene dosage dependent and influenced by temperature. using a set of soybean isolines and hybrids containing homozygous or heterozygous alleles of rsv, rsv1, rsv1-n, rsv3, or r ... | 2009 | 19126638 |
| pulmonary v gamma 4+ gamma delta t cells have proinflammatory and antiviral effects in viral lung disease. | host defenses, while effecting viral clearance, contribute substantially to inflammation and disease. this double action is a substantial obstacle to the development of safe and effective vaccines against many agents, particularly respiratory syncytial virus (rsv). rsv is a common cold virus and the major cause of infantile bronchiolitis worldwide. the role of alphabeta t cells in rsv-driven immunopathology is well studied, but little is known about the role of "unconventional" t cells. during p ... | 2009 | 19124761 |
| irregular and semi-regular polyhedral models for rous sarcoma virus cores. | whereas many viruses have capsids of uniquely defined sizes that observe icosahedral symmetry, retrovirus capsids are highly polymorphic. nevertheless, they may also be described as polyhedral foldings of a fullerene lattice on which the capsid protein (ca) is arrayed. lacking the high order of symmetry that facilitates the reconstruction of icosahedral capsids from cryo-electron micrographs, the three-dimensional structures of individual retrovirus capsids may be determined by cryo-electron tom ... | 2008 | 19122884 |
| enhancement of mucosal and cellular immune response in mice by vaccination with respiratory syncytial virus dna encapsulated with transfersome. | respiratory syncytial virus (rsv) is one of the principal causes of bronchiolitis and pneumonia in young children, and currently there is no safe and effective vaccine. dna vaccines encoding rsv surface glycoproteins are one option being examined. we evaluated the topical delivery of transfersome encapsulated dna vaccine for its ability to confer protection against rsv challenge in mice and to determine whether such delivery could induce strong and specific immunity against rsv. after topical va ... | 2008 | 19115938 |
| dscg reduces rsv-induced illness in rsv-infected mice. | respiratory syncytial virus (rsv) is one of the pathogens generally associated with the common cold, lower respiratory infection, and exacerbation of asthma. disodium cromoglycate (dscg) is a safe and widely used drug for the prevention of bronchial asthma and allergic rhinitis attacks. the effect of dscg on acute upper respiratory tract viral infections remains controversial. the purpose of the study was to investigate the effects of dscg on parameters of rsv induced-illness. using a well-chara ... | 2009 | 19107959 |
| [antibiotics prescription in infants hospitalized with respiratory syncytial virus pneumonia]. | although not useful, antibiotics are commonly prescribed in infants with viral pneumonia. | 2008 | 19107304 |
| genetic diversity of the host and severe respiratory syncytial virus-induced lower respiratory tract infection. | respiratory syncytial virus (rsv)-induced lower respiratory tract disease is a common problem in children and adults in western societies. the clinical range of rsv infection from asymptomatic to respiratory distress syndrome is believed to be the outcome of viral and host immunity interactions. genes associated with immune response are of particular interest regarding genetic predisposition to severe rsv infection. several investigators have sought to identify genetic markers for high-risk pati ... | 2009 | 19106772 |
| [construction and preliminary panning of fab phage display antibody library against respiratory syncytial virus]. | to construct a human phage display antibody library, which will help to develop new drugs and vaccines against respiratory syncytial virus (rsv) and solve many of the issues that have limited the progression and application of murine monoclonal antibodies (mcabs) in the clinic. this can provide a platform for human antibody preparation and diagnosis, prophylaxis and therapy of rsv infection in children. | 2008 | 19102829 |
| respiratory syncytial virus neutralizing antibodies in cord blood, respiratory syncytial virus hospitalization, and recurrent wheeze. | respiratory syncytial virus (rsv) hospitalization is associated with wheeze. | 2009 | 19101023 |
| [association of rantes gene promoter -28c/g polymorphism with respiratory syncytial virus bronchiolitis]. | respiratory syncytial virus (rsv) infects nearly all children under two years of age. it is poorly understood why a few children who were infected with rsv develop bronchiolitis that require hospital admission while most have a relatively minor illness. several recent studies have obtained some indications for the involvement of genetic heterogeneity in rsv bronchiolitis, implying that the clinical outcome of rsv infection perhaps is determined by genetic factors. regulated on activation, normal ... | 2008 | 19099677 |
| [lower respiratory tract infections with influenza and respiratory syncytial viruses in hospitalized elderly patients during the 2005-2006 winter season]. | to assess the proportion of lower respiratory tract infections (lrti) attributable to influenza virus and respiratory syncytial virus (rsv) during the 2005-2006 winter period, among hospitalized elderly in a geriatric unit of a french hospital near paris and and describe the characteristics of these infections. | 2009 | 19097850 |
| respiratory syncytial virus activity-- united states, july 2007-december 2008. | respiratory syncytial virus (rsv) is the most common cause of bronchiolitis and pneumonia in children aged <1 year and is a major cause of respiratory illness in older adults. rsv is transmitted person-to-person via close contact, droplets, and fomites. each year in the united states, an estimated 75,000-125,000 children aged <1 year are hospitalized with rsv. those at increased risk for hospitalization include premature infants meeting certain criteria and persons of any age with compromised re ... | 2008 | 19092759 |
| structural characterization of the rous sarcoma virus rna stability element. | in eukaryotic cells, an mrna bearing a premature termination codon (ptc) or an abnormally long 3' untranslated region (utr) is often degraded by the nonsense-mediated mrna decay (nmd) pathway. despite the presence of a 5- to 7-kb 3' utr, unspliced retroviral rna escapes this degradation. we previously identified the rous sarcoma virus (rsv) stability element (rse), an rna element downstream of the gag natural translation termination codon that prevents degradation of the unspliced viral rna. ins ... | 2009 | 19091866 |
| comprehensive detection of causative pathogens using real-time pcr to diagnose pediatric community-acquired pneumonia. | we have developed a real-time reverse transcription-pcr (rt-pcr) method to detect 13 respiratory viruses: influenza virus a and b; respiratory syncytial virus (rsv) subgroup a and b; parainfluenza virus (piv) 1, 2, and 3; adenovirus; rhinovirus (rv); enterovirus; coronavirus (oc43); human metapneumovirus (hmpv); and human bocavirus (hbov). the new method for detection of these viruses was applied simultaneously with real-time pcr for the detection of six bacterial pathogens in clinical samples f ... | 2008 | 19089556 |
| [evolution of the number of rotavirus and respiratory syncytial virus infections in children hospitalised in a french university hospital between 1998 and 2005]. | respiratory syncytial virus (rsv) and rotavirus infections represent up to 30% of cross infections in pediatric units. as they are a major public health problem, we studied their evolution and distribution at the dijon university hospital. | 2010 | 19081201 |
| high seroprevalence of human metapneumovirus infection in children in chongqing, china. | the human metapneumovirus (hmpv) is a newly discovered respiratory viral pathogen that was first isolated in 2001 in the netherlands. its global distribution and long history of infection in humans have been well documented. in this study, we assessed the seropositivity of hmpv igg antibodies in children in chongqing, china. | 2008 | 19080178 |
| lack of antibody affinity maturation due to poor toll-like receptor stimulation leads to enhanced respiratory syncytial virus disease. | respiratory syncytial virus (rsv) is a leading cause of hospitalization in infants. a formalin-inactivated rsv vaccine was used to immunize children and elicited nonprotective, pathogenic antibody. immunized infants experienced increased morbidity after subsequent rsv exposure. no vaccine has been licensed since that time. a widely accepted hypothesis attributed the vaccine failure to formalin disruption of protective antigens. here we show that the lack of protection was not due to alterations ... | 2009 | 19079256 |
| protective t cell immunity against respiratory syncytial virus is efficiently induced by recombinant bcg. | respiratory syncytial virus (rsv) is one of the leading causes of childhood hospitalization and a major health burden worldwide. unfortunately, because of an inefficient immunological memory, rsv infection provides limited immune protection against reinfection. furthermore, rsv can induce an inadequate th2-type immune response that causes severe respiratory tract inflammation and obstruction. it is thought that effective rsv clearance requires the induction of balanced th1-type immunity, involvi ... | 2008 | 19075247 |
| excess drug prescriptions during influenza and rsv seasons in the netherlands: potential implications for extended influenza vaccination. | influenza and respiratory syncytial virus (rsv) infections are responsible for considerable morbidity, mortality and health-care resource use. for the netherlands, we estimated age and risk-group specific numbers of antibiotics, otologicals and cardiovascular prescriptions per 10,000 person-years during periods with elevated activity of influenza or rsv, and compared these with peri-season rates. data were taken from the university of groningen in-house prescription database (www.iadb.nl) and vi ... | 2009 | 19071185 |
| [action of an active compound rg2-1 from radix gentianae on antirespiratory syncytial virus in vitro]. | to observe an action of an active compound rg2-1 from radix gentianae on the antirespiratory syncytial virus(rsv) in vitro. | 2008 | 19069662 |
| antiviral activities of extracts from hong kong seaweeds. | we extracted six hong kong brown seaweed species with hot water for their antiviral properties. the cytotoxicity and antiviral activity of these extracts were tested by mtt [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenlytetrezolium bromide] method, cytopathic effect reduction assay, and plaque reduction assay. the antiviral effect was further determined by flow cytometric analysis. the results showed that most of these extracts inhibited the propagation of herpes simplex virus types 1 and 2 (hsv-1 an ... | 2008 | 19067465 |