Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| complete mitochondrial genome of a natural triploid crucian carp mutant, carassius auratus var. pingxiangnensis, and phylogenetic analysis of different ploidies in crucian carp. | carassius auratus var. pingxiangnensis is a natural triploid crucian carp mutant. in order to understand its placement and genetic background at the gene level, the characteristics of mitochondrial dna sequences and phylogenetic relationship were examined. the results showed that the mitochondrial dna is a circular double-stranded dna molecule that is 16,576 bp in length with 13 protein-coding genes, 22 transfer rna genes, 2 ribosomal rna genes, and a non-coding control region. mitochondrial gen ... | 2014 | 25117343 |
| solution and high-pressure nmr studies of the structure, dynamics, and stability of the cross-reactive allergenic cod parvalbumin gad m 1. | beta-parvalbumins from different fish species have been identified as the main elicitors of ige-mediated reactions in fish-allergic individuals. here, we report for the first time the nmr determination of the structure and dynamics of the major atlantic cod (gadus morhua) allergen gad m 1 and compare them with other known parvalbumins. although the gad m 1 structure and accessibility of putative ige epitopes are similar to parvalbumins in mackerel and carp, the charge distribution at the putativ ... | 2014 | 25116395 |
| investigation of effect of 17α-ethinylestradiol on vigilin expression using an isolated recombinant antibody. | vigilin, a highly conserved protein from yeast to mammals, is a multifunctional protein in eukaryotic organisms. one biological function of vigilin is to stabilize the expression level of vitellogenin (vtg). this study aimed to develop vigilin as a new estrogen-inducible biomarker that correlates with the widely applied estrogen-inducible biomarker vtg and expand the ability to detect it in various species. here, a recombinant monoclonal antibody with high specificity against the conserved c-ter ... | 2014 | 25112681 |
| isolation and characterization of glycophorin from carp red blood cell membranes. | we isolated a high-purity carp glycophorin from carp erythrocyte membranes following extraction using the lithium diiodosalicylate (lis)-phenol method and streptomycin treatment. the main carp glycophorin was observed to locate at the position of the carp and human band-3 proteins on an sds-polyacrylamide gel. only the n-glycolylneuraminic acid (neugc) form of sialic acid was detected in the carp glycophorin. the oligosaccharide fraction was separated into two components (p-1 and p-2) using a gl ... | 2014 | 25110961 |
| therapeutic uses of animal biles in traditional chinese medicine: an ethnopharmacological, biophysical chemical and medicinal review. | forty-four different animal biles obtained from both invertebrates and vertebrates (including human bile) have been used for centuries for a host of maladies in traditional chinese medicine (tcm) beginning with dog, ox and common carp biles approximately in the zhou dynasty (c. 1046-256 bce). overall, different animal biles were prescribed principally for the treatment of liver, biliary, skin (including burns), gynecological and heart diseases, as well as diseases of the eyes, ears, nose, mouth ... | 0 | 25110425 |
| [a comparison of methods for identifying mast cells of cyprinus carpio]. | to compare different identifying methods of the mast cells of red crucian carp (cyprinus carpio). | 2014 | 25108443 |
| the effect of nc₆₀ on tissue distribution of ibuprofen in cyprinus carpio. | the potential transfer of fullerene aqueous suspension (nc₆₀) associated contaminants in vivo is one of the most significant and currently not well-understood environmental risks. in this study, the carrier function of nc₆₀ for ibuprofen (ibu) was examined through whole-body and tissue distribution determination of nc₆₀ and ibu in cyprinus carpio. the results showed that the presence of nc₆₀ only slightly increased the whole-body burden of ibu, because the amount of soluble ibu far exceeds that ... | 2014 | 25108247 |
| potential effects of hydroelectric dam development in the mekong river basin on the migration of siamese mud carp (henicorhynchus siamensis and h. lobatus) elucidated by otolith microchemistry. | the migration of siamese mud carp (henicorhynchus siamensis and h. lobatus), two of the most economically important fish species in the mekong river, was studied using an otolith microchemistry technique. fish and river water samples were collected in seven regions throughout the whole basin in thailand, laos and cambodia over a 4 year study period. there was coherence between the elements in the ambient water and on the surface of the otoliths, with strontium (sr) and barium (ba) showing the st ... | 2014 | 25099147 |
| [macroscopic and microscopic identification of amomum tsao-ko and amomum paratsao-ko fruits]. | to compare macroscopic and microscopic characteristics of the fruits of amomum tsao-ko and amomum paratsao-ko. | 2014 | 25095341 |
| generation of oxyradicals (o2. and h2o2), mitochondrial activity and induction of apoptosis of pbmc of cyprinus carpio carpio treated in vivo with halomethanes and with recombinant hsp60 kda and with lps of klebsiella pneumoniae. | halomethanes (hm) can be immunotoxic in mammals; however, in the fish immune system hm effects are unknown. in the current study, we evaluated the mitochondrial activity (ma) by mtt, induction of apoptosis by subg0 technique and quantified serum ros concentration (o2. and h2o2) and ros production in pbmc of cyprinus carpio carpio treated i.p. with ch2cl2, chcl3 and brchcl2 (0.004-40.0 mg/kg) for 96 h. positive controls were recombinant heat shock protein of 60 kda (rhsp60 kda) of klebsiella pneu ... | 2014 | 25093392 |
| complete mitochondrial genome of paramisgurnus dabryanus. | in this study, the complete mitochondrial genome of paramisgurnus dabryanus was obtained by pcr base on 18 pairs of primers. among the 18 primers, the 14 primers were from the previously published universal primers for cyprinus carpio l. mitogenome amplification. the remaining 4 primers were designed on the basis of related species mtdna sequences. the genome is 16,570 bp in length, including 2 ribosomal rna genes. 13 proteins-coding genes, 22 transfer rna genes, and a non-coding control region, ... | 2016 | 25090380 |
| micro structure analysis of the ovaries of common carp, cyprinus carpio l. inhabiting a polluted reservoir, umiam in meghalaya, india. | the study uses microscopy to analyze damage caused to the ovaries of a fresh water fish, cyprinus carpio l., owing to its exposure to municipal wastes in a polluted reservoir, umiam in meghalaya, india. histological analysis of the ovary showed atretic oocytes, detached ovarian wall, detached follicular linings, and necrosis of nuclei. scanning electron microscopy revealed deformed oocytes with a rough and distorted surface. transmission electron microscopy showed a poorly developed chorion, pre ... | 2014 | 25089745 |
| cytosolic carp promotes angiotensin ii- or pressure overload-induced cardiomyocyte hypertrophy through calcineurin accumulation. | the gene ankyrin repeat domain 1 (ankrd1) is an enigmatic gene and may exert pleiotropic function dependent on its expression level, subcellular localization and even types of pathological stress, but it remains unclear how these factors influence the fate of cardiomyocytes. here we attempted to investigate the role of carp on cardiomyocyte hypertrophy. in neonatal rat ventricular cardiomyocytes (nrvcs), angiotensin ii (ang ii) increased the expression of both calpain 1 and carp, and also induce ... | 2014 | 25089522 |
| skeletal muscle plasticity induced by seasonal acclimatization involves igf1 signaling: implications in ribosomal biogenesis and protein synthesis. | one of the most fundamental biological processes in living organisms that are affected by environmental fluctuations is growth. in fish, skeletal muscle accounts for the largest proportion of body mass, and the growth of this tissue is mainly controlled by the insulin-like growth factor (igf) system. by using the carp (cyprinus carpio), a fish that inhabits extreme conditions during winter and summer, we assessed the skeletal muscle plasticity induced by seasonal acclimatization and the relation ... | 2014 | 25088252 |
| the in vitro interference of synthetic progestogens with carp steroidogenic enzymes. | synthetic progestogens represent a class of pharmaceuticals widely used in oral contraceptives and in hormone replacement therapies. they reach the aquatic environment through wastewater effluents; however, environmental concentrations and effects on non-target organisms are poorly known. given the important role of progestogens regulating fish spawning processes, this study aimed at assessing the in vitro interference of four currently used progestogens-drospirenone (dro), levonorgestrel (lng), ... | 2014 | 25087002 |
| copper exposure induces oxidative injury, disturbs the antioxidant system and changes the nrf2/are (cuznsod) signaling in the fish brain: protective effects of myo-inositol. | the brain is the center of the nervous system in all vertebrates, and homeostasis of the brain is crucial for fish survival. copper (cu) is essential for normal cellular processes in most eukaryotic organisms but is toxic in excess. although cu is indicated as a potent neurotoxicant, information regarding its threat to fish brain and underlying mechanisms is still scarce. in accordance, the objective of this study was to assess the effects and the potential mechanism of cu toxicity by evaluating ... | 2014 | 25087001 |
| analysis of immune-related ests and differential expression analysis of few important genes in lines of rohu (labeo rohita) selected for resistance and susceptibility to aeromonas hydrophila infection. | a total of 137,629 contigs generated via de novo transcriptome assembly from resistant and susceptible lines of rohu (first generation) raised against aeromoniasis were further analyzed in terms of defence-related genes. out of 1,939 contigs showing homology to genes involved in immune processes, 1,866 were further categorised into different functional subgroups. comparative analysis revealed five genes for the first time in any carp species out of which apolipoprotein h, septin 4 isoform 3 and ... | 2014 | 25081649 |
| integrated biomarkers in wild crucian carp for early warning of water quality in hun river, north china. | metabolizing enzymes play important roles in the detoxification of various pollutants in aquatic organisms, thereby they can also be used to provide early-warning signals of environmental risks. real-time quantitative reverse-transcription polymerase chain reaction assays were developed to quantify cytochrome p450 1a (cyp1a), superoxide dismutase (sod), glutathione peroxidase (gpx), catalase (cat), and glutathione-s-transferase (gst) in crucian carp (carassius auratus). the methods were then use ... | 2014 | 25079422 |
| protection of common carp (cyprinus carpio l.) spermatozoa motility under oxidative stress by antioxidants and seminal plasma. | the protective influence of seminal plasma and the antioxidants catalase (cat), superoxide dismutase (sod), and glutathione (gth) on quality parameters, oxidative stress indices, and antioxidant activity was studied in common carp (cyprinus carpio) spermatozoa exposed to the xanthine-xanthine oxidase (x-xo) system. fish spermatozoa were incubated for 5 and 20 min at 4 °c with x-xo concentrations of 1 mm x-0.1 u/ml, 0.6 mm x-0.05 u/ml, 0.3 mm x-0.025 u/ml, and 0.1 mm x-0.0125 u/ml. a dose-depende ... | 2014 | 25079247 |
| qtl mapping for economically important traits of common carp (cyprinus carpio l.). | quantitative trait loci (qtl) were analyzed for three economically important traits, i.e., body weight (bw), body length (bl), and body thickness (bt), in an f1 family of common carp holding the 190 progeny. a genetic linkage map spanning 3,301 cm in 50 linkage groups with 627 markers and an average distance of 5.6 cm was utilized for qtl mapping. sixteen qtls associated with all three growth-related traits were scattered across ten linkage groups, lg6, lg10, lg17, lg19, lg25, lg27, lg28, lg29, ... | 2015 | 25078056 |
| determination of pesticide residues in fish tissues by modified quechers method and dual-d-spe clean-up coupled to gas chromatography-mass spectrometry. | the aim of this research was to modify the quick, easy, cheap, effective, rugged and safe (quechers) method for the determination of organochlorine and organophosphate pesticides in fatty animal matrices such as fish muscle tissues of carp and sturgeon collected from carp valley, lesser poland. pesticides extraction effectiveness was evaluated at 0.030 mg kg(-1) spiking level and efficiency of the dispersive-solid-phase extraction (d-spe) clean-up step was evaluated by comparison testing two dif ... | 2015 | 25074831 |
| pathogenesis of acute and chronic diseases caused by cyprinid herpesvirus-3. | the pathogenesis of cyprinid herpesvirus-3 (cyhv-3) was studied using different lineages of carp/koi. after exposure to the virus, infected cells were first found in the skin by histopathology and by in situ hybridization. the epidermis of the skin was most severely damaged and often sloughed off in the fish sampled on days 5 through 8, and the fish that were highly sensitive to the virus died within 8 or 10 days after infection. serum osmolality of the infected fish, particularly just before de ... | 2015 | 25073413 |
| effectiveness of a sediment time critical removal action-pcb reduction in fish tissue, surface water, and sediment via wet excavation. | documenting successful remediation of polychlorinated biphenyl (pcb)-contaminated sediments is limited; potentially due to inadequate monitoring methods, complexities associated with the environment, and selected remedial techniques. at some sites, absence of appropriate baseline and postremoval monitoring limits proper evaluation of remedial efficacy. accurate interpretation of interactions between media, space, time, species, lipid content, and remedial technique requires robust study design a ... | 2015 | 25070026 |
| toll-like receptor 22 in labeo rohita: molecular cloning, characterization, 3d modeling, and expression analysis following ligands stimulation and bacterial infection. | toll-like receptors (tlrs) are a class of innate immune receptors that sense pathogens or their molecular signatures and activate signaling cascades to induce a quick and non-specific immune response in the host. among various types of tlrs, tlr22 is exclusively present in teleosts and amphibians and is expected to play the distinctive role in innate immunity. this report describes molecular cloning, three-dimensional (3d) modeling, and expression analysis of tlr22 in rohu (labeo rohita), the mo ... | 2014 | 25064133 |
| additive effects of predator cues and dimethoate on different levels of biological organisation in the non-biting midge chironomus riparius. | the combined effects of a pesticide and predation risk on sublethal endpoints in the midge chironomus riparius were investigated using a combination of predator-release kairomones from common carp (cyprinus carpio) and alarm substances from conspecifics together with the pesticide dimethoate. midge larvae were exposed for 30 days to three sublethal dimethoate concentrations (0.01, 0.1 and 0.25 mg l(-1)) in the presence or absence of predator cues. sublethal endpoints were analysed at different l ... | 2014 | 25063887 |
| effects of atrazine and chlorpyrifos on dna methylation in the liver, kidney and gill of the common carp (cyprinus carpio l.). | pesticide exposure has repeatedly been associated with cancers, although the molecular mechanisms behind this association are largely undetermined. abnormal dna methylation plays a key role in the process of some disease. however, little was known about the effect of pesticides on dna methylation in the common carp. in this study, we investigated the mrna levels of dna methyltransferases (dnmts) and methyl-cpg-binding protein dna-binding domain protein 2 (mbd2) as well as the dna methylation lev ... | 2014 | 25062446 |
| analyzing cold tolerance mechanism in transgenic zebrafish (danio rerio). | low temperatures may cause severe growth inhibition and mortality in fish. in order to understand the mechanism of cold tolerance, a transgenic zebrafish tg (smyd1:m3ck) model was established to study the effect of energy homeostasis during cold stress. the muscle-specific promoter smyd1 was used to express the carp muscle form iii of creatine kinase (m3-ck), which maintained enzymatic activity at a relatively low temperature, in zebrafish skeletal muscle. in situ hybridization showed that m3-ck ... | 2014 | 25058652 |
| differential effects of lipid fractions from silver carp brain on human cervical carcinoma cells in vitro. | previous research has revealed that n3 polyunsaturated fatty acids (pufas) exhibit anticancer activities. lipids from a fish brain contain substantial n3 pufas. however, no research has been conducted on the action and mechanism of their potent anticancer activities. in this study, total lipids (tls) from silver carp brain were isolated into polar lipids (pls) and neutral lipids (nls), and the anticancer potential of the lipid fractions (lfs) was investigated using the human cervical carcinoma h ... | 2014 | 25047635 |
| autophagy-inducing peptides from mammalian vsv and fish vhsv rhabdoviral g glycoproteins (g) as models for the development of new therapeutic molecules. | it has not been elucidated whether or not autophagy is induced by rhabdoviral g glycoproteins (g) in vertebrate organisms for which rhabdovirus infection is lethal. our work provides the first evidence that both mammalian (vesicular stomatitis virus, vsv) and fish (viral hemorrhagic septicemia virus, vhsv, and spring viremia carp virus, svcv) rhabdoviral gs induce an autophagic antiviral program in vertebrate cell lines. the transcriptomic profiles obtained from zebrafish genetically immunized w ... | 2014 | 25046110 |
| physical changes in specimens of five species of cyprinidae preserved in ethanol and frozen. | preservation in 30% ethanol and freezing to a temperature of -20 ± 2° c is an appropriate method for measurement of fish eggs, larvae and juveniles. egg diameter of the common carp cyprinus carpio increased insignificantly by 1·32% after preservation compared with live size. the total length (lt ) of 1 day post-hatching (dph) larvae as well as the standard length (ls) of 16 dph larvae of c. carpio increased significantly (2·95 and 1·50%, respectively) after preservation. egg diameter as well as ... | 2014 | 25041177 |
| occurrence of additive brominated flame retardants in aquatic organisms from tai lake and yangtze river in eastern china, 2009-2012. | since the phase-out of pbdes, reports regarding occurrences of these compounds in the environment have become less frequent. to characterize potential influences of the phase-out of pbdes' on concentrations in the environment, trends in concentrations as a function of time were investigated for two additive brominated flame retardants, pbdes and hbcds. three aquatic species, including shrimp, common carp, and yellow catfish, were collected from meiliang bay of tai lake, 2009-2012. the analysis o ... | 2014 | 25038592 |
| up-regulation of nuclear factor e2-related factor 2 upon svcv infection. | nuclear factor e2 - related factor 2 (nrf2) is a crucial transcription factor that regulates the basal and inducible expression of many antioxidant response element (are)-dependent genes, including heme oxygenase-1 (ho-1) and superoxide dismutase 1 (sod1). the nrf2/are pathway has been regarded as a critical switch in the initiation of cellular defence systems for surviving oxidative insults and viral infection. in this study, the nrf2 gene of epc cells, which is originally derived from pimephal ... | 2014 | 25038284 |
| bioaccumulation, distribution and metabolism of bde-153 in the freshwater fish carassius auratus after dietary exposure. | polybrominated diphenyl ethers (pbdes) are of great environmental concern due to bioaccumulation and biomagnification in different food chains. however, significant biotransformation of some congeners via reductive debromination has been observed during in vivo and in vitro laboratory exposures. little is known about the fate of 2,2',4,4',5,5'-hexabromodiphenyl ether (bde-153) in fish. in the present study, crucian carp (carassius auratus) were exposed to bde-153 at a concentration of 10μg/g in ... | 2014 | 25038267 |
| characterization and expression pattern of a novel β-defensin in common carp (cyprinus carpio l.): implications for its role in mucosal immunity. | β-defensins are a group of cysteine-rich cationic antimicrobial peptides that play antibacterial and antiviral roles in immune systems of vertebrates. here, we report the cloning and identification of a β-defensin 3 cdna sequence from the common carp (cyprinus carpio l.). sequence alignment and phylogenetic analysis indicated that this β-defensin 3 belonged to the bd-2 group of fish. real-time pcr showed that the β-defensin 3 mrna was expressed in all the tissues of normal common carp that we ex ... | 2014 | 25036829 |
| characterization and expression analysis of an interferon-γ2 induced chemokine receptor cxcr3 in common carp (cyprinus carpio l.). | chemokine and chemokine receptor signalling pairs play a crucial role in regulation of cell migration, morphogenesis, and cell activation. expressed in mammals on activated t and nk cells, chemokine receptor cxcr3 binds interferon-γ inducible chemokines cxcl9-11 and ccl21. here we sequenced the carp cxcr3 chemokine receptor and showed its relationship to cxcr3a receptors found in other teleosts. we found high expression of the cxcr3 gene in most of the organs and tissues of the immune system and ... | 2014 | 25036761 |
| pierisins and carp-1: adp-ribosylation of dna by artcs in butterflies and shellfish. | the cabbage butterfly, pieris rapae, and related species possess a previously unknown adp-ribosylating toxin, guanine specific adp-ribosyltransferase. this enzyme toxin, known as pierisin, consists of enzymatic n-terminal domain and receptor-binding c-terminal domain, or typical ab-toxin structure. pierisin efficiently transfers an adp-ribosyl moiety to the n(2) position of the guanine base of dsdna. receptors for pierisin are suggested to be the neutral glycosphingolipids, globotriaosylceramide ... | 2015 | 25033755 |
| mechanisms of neuroblastoma cell growth inhibition by carp-1 functional mimetics. | neuroblastomas (nbs) are a clinically heterogeneous group of extra cranial pediatric tumors. patients with high-risk, metastatic nbs have a long-term survival rate of below 40%, and are often resistant to current therapeutic modalities. due to toxic side effects associated with radiation and chemotherapies, development of new agents is warranted to overcome resistance and effectively treat this disease in clinic. carp-1 functional mimetics (cfms) are an emerging class of small molecule compounds ... | 2014 | 25033461 |
| gill remodeling in three freshwater teleosts in response to high environmental ammonia. | the present study aimed to determine whether gill macro- and microstructure show compensatory responses in three freshwater fish differing in their sensitivity to high environmental ammonia (hea). the highly ammonia-sensitive salmonid oncorhynchus mykiss (rainbow trout), the less ammonia-sensitive cyprinid cyprinus carpio (common carp) and the highly ammonia-resistant cyprinid carassius auratus (goldfish) were used as test species and were exposed for 0 h (control), 3h, 12h, 24h, 48 h, 84 h and ... | 2014 | 25033244 |
| the natural history of adp-ribosyltransferases and the adp-ribosylation system. | catalysis of nad(+)-dependent adp-ribosylation of proteins, nucleic acids, or small molecules has evolved in at least three structurally unrelated superfamilies of enzymes, namely adp-ribosyltransferase (art), the sirtuins, and probably tm1506. of these, the art superfamily is the most diverse in terms of structure, active site residues, and targets that they modify. the primary diversification of the art superfamily occurred in the context of diverse bacterial conflict systems, wherein arts pla ... | 2015 | 25027823 |
| duplication and differentiation of common carp (cyprinus carpio) myoglobin genes revealed by bac analysis. | two distinct myoglobin (mb) transcripts have been reported in common carp, cyprinus carpio, which is a hypoxia-tolerant fish living in habitats with greatly fluctuant dissolved oxygen levels. recombinant protein analysis has shown functional specialization of the two mb transcripts. in this work, analysis for mb-containing bacterial artificial chromosome (bac) clones indicated different genome loci for common carp myoglobin-1 (mb-1) and myoglobin-2 (mb-2) genes. fluorescence in situ hybridizatio ... | 2014 | 25026501 |
| antibodies specific for carbamylated proteins precede the onset of clinical symptoms in mice with collagen induced arthritis. | the immune response to post-translationally modified antigens is a key characteristic of rheumatoid arthritis. carbamylation is such a posttranslational modification. recently, we demonstrated that autoantibodies recognizing carbamylated proteins are present in sera of rheumatoid arthritis. the molecular mechanisms underlying the break of tolerance and hence the induction of anti-carp antibody responses are unknown as well as their appearance in mouse models for systemic arthritis. therefore we ... | 2014 | 25025869 |
| pouchitis: prevention and treatment. | pouchitis, representing a spectrum of disease phenotypes, is the most common long-term complication in patients who have undergone restorative proctocolectomy with ileal pouch-anal anastomosis (ipaa). its management and prevention are challenging. | 2014 | 25023187 |
| characterization and expression analysis of two distinct neuropeptide ya paralogues in jian carp (cyprinus carpio var. jian). | two distinct neuropeptide ya paralogues (jlnpya1 and jlnpya2) were cloned and characterized in jian carp (cyprinus carpio var. jian), with a highly conserved organization encoded by four exons and three introns. the cdnas for jlnpya1 and jlnpya2 were 693 and 730 bp in size, respectively. jlnpya1 and jlnpya2 both encoded a 96-amino acid protein, which shared 97.9 % identity. phylogenetic tree showed that it has two npya genes, called jlnpya1 and jlnpya2, that presumably resulted from the tetraplo ... | 2014 | 25015546 |
| interaction of diet and the masou salmon δ5-desaturase transgene on δ6-desaturase and stearoyl-coa desaturase gene expression and n-3 fatty acid level in common carp (cyprinus carpio). | the masou salmon δ5-desaturase-like gene (d5d) driven by the common carp β-actin promoter was transferred into common carp (cyprinus carpio) that were fed two diets. for p1 transgenic fish fed a commercial diet, δ6-desaturase-like gene (d6d) and stearoyl-coa desaturase (scd) mrna levels in muscle were up-regulated (p < 0.05) 12.7- and 17.9-fold, respectively, and the d6d mrna level in the gonad of transgenic fish was up-regulated 6.9-fold (p < 0.05) compared to that of non-transgenic fish. in co ... | 2014 | 25011564 |
| protective effect of shrimp carotenoids against ammonia stress in common carp, cyprinus carpio. | this study is aimed at evaluating the protective effect of shrimp carotenoids on ammonia stress in common carp. crude carotenoid extract from shrimp exoskeleton, astaxanthin and astaxanthin ester fractionated from crude extract was fed to the common carp fingerlings at 100 and 200ppm concentration by incorporating carotenoids into feed. experimental and control fish were then exposed to sublethal dose of ammonia. serum total antioxidant status (tas), activities of superoxide dismutase (sod) and ... | 2014 | 25011116 |
| bioaccumulation and risk assessment of per- and polyfluoroalkyl substances in wild freshwater fish from rivers in the pearl river delta region, south china. | per- and polyfluoroalkyl substances (pfass) are used in various industries, which results in their ubiquitous occurrence in the environment. this study determined the concentrations of eighteen pfass in muscle and liver of nine wild freshwater fish species collected from rivers in the pearl river delta (prd) region, south china, and assessed their bioaccumulation and potential health risks to local people. the results showed that eight and twelve pfass were detected in the fish muscle and liver ... | 2014 | 25011114 |
| perfluoroalkyl acid contamination and polyunsaturated fatty acid composition of french freshwater and marine fishes. | in this study, french marine and freshwater fish perfluoroalkyl acid (pfaa) contamination are presented along with their fatty acid (fa) composition to provide further elements for a risk/benefit balance of fish consumption to be assessed. the 29 most consumed marine fish species were collected in four metropolitan french coastal areas in 2004 to constitute composite samples. geographical differences in terms of consumed species and contamination level were taken into account. three hundred and ... | 2014 | 25004121 |
| first evidence of comparative responses of toll-like receptor 22 (tlr22) to relatively resistant and susceptible indian farmed carps to argulus siamensis infection. | toll-like receptor 22 (tlr22) is present in teleost but not in mammals. among indian farmed carps, catla catla is relatively more resistant than labeo rohita to argulus siamensis lice infection. tlr22 is believed to be associated with innate immunity against ectoparasite infection. to investigate the tlr22 mediated immunity against argulosis, we have cloned and characterized tlr22 genes of l. rohita (rtlr22) and c. catla (ctlr22). the full-length cdnas of rtlr22 and ctlr22 contained an open read ... | 2014 | 24998226 |
| the bioaccumulation and biotransformation of synthetic estrogen quinestrol in crucian carp. | the occurrence and fate of endocrine disrupting chemicals (edcs) in aquatic species have attracted close attention during the last decades. in this study, the bioaccumulation and biotransformation of synthetic estrogen quinestrol, one of the typical edcs, in the plasma and liver of crucian carp, were investigated by a newly developed and validated reversed-phase high performance liquid chromatography with fluorescent detection method. crucian carp were exposed to quinestrol in concentration of 2 ... | 2014 | 24997302 |
| c-reactive protein and complement as acute phase reactants in common carp cyprinus carpio during cyhv-3 infection. | cyprinid herpesvirus 3 (cyhv-3) is the aetiological agent of a highly virulent and lethal disease of common carp cyprinus carpio and its ornamental koi varieties. however, specific knowledge about immune mechanisms behind the infection process is very limited. we aimed to evaluate the effect of the cyhv-3 infection on the profile of 2 major components of the common carp immune acute phase response: the c-reactive protein (crp) and the complement system. common carp were infected with cyhv-3 by b ... | 2014 | 24991845 |
| identification of promoter within the first intron of plzf gene expressed in carp spermatogonial stem cells. | promyelocytic leukemia zinc finger (plzf), a transcriptional repressor, is involved in survival and maintenance of pluripotent stem cells including embryonic and spermatogonial stem cells in mammals. its cdna was characterized and expression in proliferating spermatogonial stem cells of rohu (labeo rohita), a farmed carp, was documented. in teleost, the information on its promoter activity is lacking. here, we have isolated, sequenced and performed the first characterization of regulatory elemen ... | 2014 | 24990695 |
| toxic effects of paraquat on cytokine expression in common carp, cyprinus carpio l. | in this study, the acute toxicity of paraquat (pq) in common carp was determined. then, the contents and mrna levels of the cytokines interleukin-1β (il-1β), interferon-γ (ifn-γ), and tumor necrosis factor-α (tnf-α) were evaluated following subacute exposure to pq. the results show that the lc50 of pq for common carp at 72 and 96 h was 15.962 and 15.106 mg/l, respectively. moreover, after 7 days of subacute pq exposure, the il-1β content in the fish liver and kidney increased, although it decrea ... | 2014 | 24989943 |
| mapping qtls for swimming ability related traits in cyprinus carpio l. | body height (bh), head length (hl), snout length (sl), and tail length (tl) are important traits related with swimming ability of fish. therefore, improving these traits will increase the production which is the basic goal of aquaculture breeding. to understand the genetic basis of swimming ability related traits in cyprinus carpio l., a high-density linkage map spanning 3,301 cm in 50 linkage groups was utilized for quantitative trait locus (qtl) mapping. mapping family comprised 190 offspring ... | 2014 | 24985693 |
| a linkage map of transcribed single nucleotide polymorphisms in rohu (labeo rohita) and qtl associated with resistance to aeromonas hydrophila. | production of carp dominates world aquaculture. more than 1.1 million tonnes of rohu carp, labeo rohita (hamilton), were produced in 2010. aeromonas hydrophila is a bacterial pathogen causing aeromoniasis in rohu, and is a major problem for carp production worldwide. there is a need to better understand the genetic mechanisms affecting resistance to this disease, and to develop tools that can be used with selective breeding to improve resistance. here we use a 6 k snp array to genotype 21 full-s ... | 2014 | 24984705 |
| effects of different concentrations of salt and sugar on biogenic amines and quality changes of carp (cyprinus carpio) during chilled storage. | biogenic amines have gained a great deal of attention due to their toxic potential in humans. carp is one of the most important freshwater fish species in china. salt and sugar are capable of preserving food. there is a limited amount of information on the changes of biogenic amines in freshwater fish influenced by salt and sugar. this study aimed to detect the changes in biogenic amines, sensory attributes, total volatile basic nitrogen (tvb-n) and total viable counts (tvc) of carp influenced b ... | 2015 | 24976408 |
| characterizing the transcriptome of yellow-cheek carp (elopichthys bambusa) enables evolutionary analyses within endemic east asian cyprinidae. | the identification of genes that may be responsible for the divergence of closely related species is one of the central goals of evolutionary biology. the species of endemic east asian cyprinidae diverged less than 8millionyears ago, and the morphological differences among these species are great. however, the genetic basis of their divergence remains unknown. in this report, we investigated the transcriptome of one endemic east asian cyprinid - the yellow-cheek carp elopichthys bambusa. a compa ... | 2014 | 24973763 |
| modulation of biochemical indices in common carp (cyprinus carpio l.) under the influence of toxic cyanobacterial biomass in diet. | cyanobacteria are producers of potent and environmentally abundant microcystins, representing an emerging global health issue. in the present study, we investigated the impact of cyanobacterial biomass on biochemical indices of common carp (cyprinus carpio l., average weight of 246 ± 73 g) under laboratory conditions. the fish were fed a diet containing cyanobacterial biomass with microcystins in high concentration (0.4 mg/kg of fish weight and day) for 28 days. statistical evaluation of the inf ... | 2014 | 24972534 |
| potential human health risk by metal(loid)s, 234,238u and 210po due to consumption of fish from the "luis l. leon" reservoir (northern méxico). | concentrations of as, cu, fe, hg, pb and zn and activity concentrations from 234,238u and 210po in water, fillet, liver and gills were determined in three stocked fish species from the luis l. leon reservoir, located in northern mexico. the considered species were lepomis cyanellus, cyprinus carpio and ictalurus furcatus. 238u and 234u activity concentration (ac) in fillet samples showed values of 0.007-0.014 and 0.01-0.02 bq∙kg-1 wet weight (ww), respectively. liver samples for l. cyanellus, c. ... | 2014 | 24968208 |
| identification of a novel fish granzyme involved in cell-mediated immunity. | granzymes (gzms) are serine proteases released from cytoplasmic granules within cytotoxic t lymphocytes and natural killer (nk) cells. gzms induce apoptosis within virus-infected and transformed cells. in fish as well as mammals, gzms appear to play a major role in inducing target cell death. however, information on the function of fish gzms is limited, although gzm-like genes have been reported in several species. we identified and characterized a fish gzm (termed gcgzm) in ginbuna crucian carp ... | 2014 | 24968079 |
| interaction between dopamine and neuropeptide y in the telencephalon of the indian major carp, cirrhinus cirrhosus. | in teleosts, while neuropeptide y (npy) has emerged as one of the potent regulators of gnrh-lh axis, entopeduncular nucleus (en) in the ventral telencephalon serves as major site for npy synthesis/storage. neurons of the en innervate preoptic area and pituitary, respond to gonadal steroids, undergo reproduction phase-related changes, and are believed to convey sex steroid-borne information to gnrh neurons. in spite of the importance of en, the neural circuitry associated with the nucleus has not ... | 2015 | 24967949 |
| changes in physiochemical properties of water-soluble proteins from crucian carp (carassius auratus) during heat treatment. | in order to understand physicochemical properties of water-soluble proteins obtained from crucian carp, turbidity, total sulfhydryl content, hydrophobicity and sds-page of crucian carp water-soluble proteins during heat treatment were investigated. turbidity remained unchanged up to 44°c and considerably increased from 46°c to 54°c, one peak of increase rate was found at 50°c; total sh content decreased rapidly when heated from 50°c to 55°c; hydrophobicity increased sharply when heated up to 45° ... | 2014 | 24966436 |
| ifn regulatory factor 10 is a negative regulator of the ifn responses in fish. | ifn regulatory factor (irf) 10 belongs to the irf family and exists exclusively in birds and fish. most irfs have been identified as critical regulators in the ifn responses in both fish and mammals; however, the role of irf10 is unclear. in this study, we identified irf10 in zebrafish (danio rerio) and found that it serves as a negative regulator to balance the innate antiviral immune responses. zebrafish irf10 (drirf10) was induced by intracellular polyinosinic:polycytidylic acid in zf4 (zebra ... | 2014 | 24958903 |
| assessment of microwave cooking on the bioaccessibility of cadmium from various food matrices using an in vitro digestion model. | bioaccessibility represents the maximum amount of pollutant ingested with food that is available for intestinal absorption. the measurement of bioaccessibility can achieve a more accurate risk assessment. thus, in this study, the bioaccessibility of raw/microwave-cooked store-bought food including carrot, potato, white radish, lotus root, sweet corn, long grain rice, soybean, fleshy prawn, eastern oyster, kelp, and common carp were investigated by applying an in vitro digestion method. a validat ... | 2014 | 24958019 |
| gnih and gnrh expressions in the central nervous system and pituitary of indian major carp, labeo rohita during ontogeny: an immunocytochemical study. | gonadotropin-releasing hormone (gnrh) is the major hypothalamic neuropeptide stimulating gonadotropin secretion in vertebrates. in 2000, gonadotropin-inhibitory hormone (gnih) was discovered as a hypothalamic neuropeptide that inhibits gonadotropin secretion in birds. subsequent studies have shown that gnih is present in the brain of other vertebrates. we show for the first time gnih immunoreactivity in the central nervous system and pituitary during development of indian major carp, labeo rohit ... | 2015 | 24955881 |
| immune response and disease resistance of carotenoids supplementation diet in cyprinus carpio against aeromonas hydrophila. | the effect of carotenoid-supplementation diet on immune response and disease resistance in common carp, cyprinus carpio against aeromonas hydrophila at weeks 1, 2, and 4 is reported. the cumulative mortality was 10% when fish were fed with 50 or 100 mg kg(-1) supplementation diets while the un-supplementation diet treated group suffered 90% mortality against the pathogen. the phagocytic activity and complement activity significantly increased with 50 and 100 mg kg(-1) diet groups from weeks 2 an ... | 2014 | 24954837 |
| genomic evidence of homologous recombination in spring viremia of carp virus: a negatively single stranded rna virus. | a new strain of spring viraemia of carp virus, denominated svcv-265, was isolated from an ornamental common carp (cyprinus carpio) in shanghai, china, 2013. the isolate could produce obvious cytopathic effects on epc cells, while was shown to be of low virulence for juvenile koi. complete genome sequencing revealed the genome of the svcv-265 strain is 11,029 nucleotides in length and phylogenetic analysis showed the isolate was clustered within asia clade but was divergent from chinese a1, a2 an ... | 2014 | 24954789 |
| genotoxicological response of the common carp (cyprinus carpio) exposed to spring water in tlaxcala, méxico. | this study evaluated the genotoxic impact of anthropic activities in huactzinco spring, using cyprinus carpio as a biomonitor. in situ and in vivo experimental designs were compared by means of simultaneous 2-week exposures. the water from the spring generated mean micronuclei frequency values (108.6 ± 32 mn/1,000) and dna fragmentation values (143.4 ± 35 au) which were statistically higher than those for the negative control (10.9 ± 6 mn/1,000 and 67.6 ± 23 au). the in situ and in vivo experime ... | 2014 | 24950945 |
| innate immune response of indian major carp, labeo rohita infected with oomycete pathogen aphanomyces invadans. | the fish pathogenic oomycete aphanomyces invadans is the causative agent of epizootic ulcerative syndrome (eus), a fish disease of international significance and reportable to the world organisation for animal health. in spite of the current and potential impact of a. invadans infection on fisheries and aquaculture sectors of the world, very little is known about the host-a. invadans interactions. in the present study, following experimental infection with a. invadans in one of the indian major ... | 2014 | 24945572 |
| epidemiology of fishborne trematodiasis in kazakhstan. | fish borne trematodes are an important cause of morbidity in kazakhstan. the number of human cases of opisthorchiidosis (infection with parasites of the family opisthorchiidae) reached a peak of 2521 recorded cases (17 cases per 100,000) in 2002 with a gradual decline to 1225 cases (7.4 cases per 100,000) in 2011. most human cases are found in the north and north east part of kazakhstan in areas drained by the irtysh river and it tributaries. a further focus is found in the north west in the ura ... | 2014 | 24943190 |
| the activity of carp muscle-specific creatine kinase at low temperature is enhanced by decreased hydrophobicity of residue 268. | abstract the muscle-specific forms of creatine kinase in rabbit (rm-ck) and carp (m1-ck) exhibit different temperature-dependent functional properties. replacing the glycine at residue 268 of rm-ck with asparagine increases the enzyme's activity at 10°c. in this study, we investigated how hydrophobicity of residue 268 affects the biochemical properties of rm-ck and m1-ck at low temperature. we generated three mutants of both rm-ck and m1-ck: asp268, lys268, and leu268. the secondary structures o ... | 2014 | 24940915 |
| immune responses and protective efficacy of recombinant outer membrane protein r (rompr)-based vaccine of aeromonas hydrophila with a modified adjuvant formulation in rohu (labeo rohita). | despite the importance and success of developing a candidate vaccine against aeromonas hydrophila infection in fish, little is known about the molecular mechanisms of the vaccine-induced immunoprotection in indian major carp, labeo rohita, primarily due to lack of information on most of the immune related genes of the species. in this study, a novel candidate antigen recombinant outer membrane protein r (rompr) of a. hydrophila was evaluated as a vaccine candidate along with a modified adjuvant ... | 2014 | 24937805 |
| a new growth curve model for biological growth: some inferential studies on the growth of cirrhinus mrigala. | growth of living organisms is a fundamental biological process. it depicts the physiological development of the species related to the environment. mathematical development of growth curve models has a long history since its birth. we propose a mathematical model to describe the evolution of relative growth rate as a function of time based on a real life experiment on a major indian carp cirrhinus mrigala. we establish that the proposed model is able to describe the fish growth dynamics more acc ... | 2014 | 24933474 |
| in vitro glucuronidation kinetics of deoxynivalenol by human and animal microsomes and recombinant human ugt enzymes. | the mycotoxin deoxynivalenol (don), formed by fusarium species, is one of the most abundant mycotoxins contaminating food and feed worldwide. upon ingestion, the majority of the toxin is excreted by humans and animal species as glucuronide conjugate. first in vitro data indicated that don phase ii metabolism is strongly species dependent. however, kinetic data on the in vitro metabolism as well as investigations on the specific enzymes responsible for don glucuronidation in human are lacking. in ... | 2015 | 24927789 |
| draft genome sequence of aeromonas hydrophila strain ae34, isolated from a septicemic and moribund koi carp (cyprinus carpio koi), a freshwater aquarium fish. | aeromonas hydrophila is an important opportunistic pathogen that infects a variety of aquatic and terrestrial animals, including humans. we report here the draft genome sequence of a. hydrophila ae34, a multidrug-resistant isolate from the kidney of a moribund koi carp (ciprinus carpio koi) with signs of hemorrhagic septicemia. | 2014 | 24926056 |
| mechanisms involved in apoptosis of carp leukocytes upon in vitro and in vivo immunostimulation. | during inflammation leukocyte activity must be carefully regulated, as high concentrations and/or prolonged action of pro-inflammatory mediators e.g. reactive oxygen species (ros) can be detrimental not only for pathogens but also for host tissues. programmed cell death - apoptosis is a most effective regulatory mechanism for down regulation of leukocyte activity, but little is known about this process in fish. we aimed to reveal the mechanisms of initiation and regulation of apoptosis in carp n ... | 2014 | 24925760 |
| examination of the early infection stages of koi herpesvirus (khv) in experimentally infected carp, cyprinus carpio l. using in situ hybridization. | koi herpesvirus (khv) causes a highly infectious disease afflicting common carp and koi, cyprinus carpio l. various molecular and antibody-based detection methods have been used to elucidate the rapid attachment and dissemination of the virus throughout carp tissues, facilitating ongoing development of effective diagnostic approaches. in situ hybridization (ish) was used here to determine the target tissues of khv during very early infection, after infecting carp with a highly virulent khv isola ... | 2015 | 24925228 |
| the interaction between conditioned fish and naive schools of juvenile carp (cyprinus carpio, pisces). | in a circular experimental pool the behaviour of trained juvenile carp placed in naive schools of different size and of different behavioural motivation was studied. it could be shown that the time the trained fish needed to arrive at the feeding place was much shorter when it swam in a habituated school than in a frightened one. this is discussed as a result of the conflict between the conditioned reaction and the various levels of mutual attraction. in the same manner the time elapsing from cs ... | 1976 | 24923748 |
| caffeine-induced ca2+ oscillations in type i horizontal cells of the carp retina and the contribution of the store-operated ca2+ entry pathway. | the mechanisms of release, depletion, and refilling of endoplasmic reticulum (er) ca2+ were investigated in type i horizontal cells of the carp retina using a fluo-3-based ca2+ imaging technique. exogenous application of caffeine, a ryanodine receptor agonist, induced oscillatory intracellular free ca2+ concentration ([ca2+]i) responses in a duration- and concentration-dependent manner. in ca2+-free ringer's solution, [ca2+]i transients could also be induced by a brief caffeine application, wher ... | 2014 | 24918937 |
| metals and nonsteroidal anti-inflammatory pharmaceuticals drugs present in water from madín reservoir (mexico) induce oxidative stress in gill, blood, and muscle of common carp (cyprinus carpio). | many toxic xenobiotics that enter the aquatic environment exert their effects through redox cycling. oxidative stress, which incorporates both oxidative damage and antioxidant defenses, is a common effect induced in organisms exposed to xenobiotics in their environment. the results of the present study aimed to determine the oxidative stress induced in the common carp cyprinus carpio by contaminants [metals and nonsteroidal anti-inflammatory drugs (nsaids)] present in madín reservoir. five sampl ... | 2014 | 24916851 |
| tissue distribution, bioconcentration, metabolism, and effects of erythromycin in crucian carp (carassius auratus). | in this study, the tissue distribution, bioconcentration, metabolism and biological effects of the macrolide antibiotic erythromycin (ery) were investigated in fish using crucian carp (carassius auratus) as a model. crucian carp were exposed to various concentrations of ery (4, 20, and 100 μg/l) for 28 days. the uplc/ms/ms analysis of both water and tissue provided the bioconcentration of ery and its metabolites in the fish body. the results from tissue samples showed that a maximum tissue conce ... | 2014 | 24911771 |
| construction and analysis of high-density linkage map using high-throughput sequencing data. | linkage maps enable the study of important biological questions. the construction of high-density linkage maps appears more feasible since the advent of next-generation sequencing (ngs), which eases snp discovery and high-throughput genotyping of large population. however, the marker number explosion and genotyping errors from ngs data challenge the computational efficiency and linkage map quality of linkage study methods. here we report the highmap method for constructing high-density linkage m ... | 2014 | 24905985 |
| identification of b cells as a major site for cyprinid herpesvirus 3 latency. | cyprinid herpesvirus 3 (cyhv-3), commonly known as koi herpesvirus (khv), is a member of the alloherpesviridae, and is a recently discovered emerging herpesvirus that is highly pathogenic for koi and common carp. our previous study demonstrated that cyhv-3 becomes latent in peripheral white blood cells (wbc). in this study, cyhv-3 latency was further investigated in igm(+) wbc. the presence of the cyhv-3 genome in igm(+) wbc was about 20-fold greater than in igm(-) wbc. to determine whether cyhv ... | 2014 | 24899202 |
| susceptibility of koi and yellow perch to infectious hematopoietic necrosis virus by experimental exposure. | infectious hematopoietic necrosis virus (ihnv) is a novirhabdoviral pathogen that originated in western north america among anadromous pacific salmonids. severe disease epidemics in the late 1970s resulting from ihnv's invasion into farmed rainbow trout oncorhynchus mykiss in north america, asia, and europe emphasized ihnv's ability to adapt to new hosts under varying rearing conditions. yellow perch perca flavescens and koi carp cyprinus carpio (hereafter, "koi") are aquaculture-reared fish tha ... | 2014 | 24895860 |
| in vitro inhibition of cyprinid herpesvirus-3 replication by rnai. | cyprinid herpesvirus-3 (cyhv-3) is an etiological agent of a notifiable disease that causes high mortality rates affecting both the common and koi carp cyprinus carpio l. there is no current treatment strategy to save cyhv-3 infected fish. rna mediated interference (rnai) is an emerging strategy used for understanding gene function and is a promising method in developing novel therapeutics and antiviral medications. for this study, the possibility of activating the rnai pathway by the use of sma ... | 2014 | 24893110 |
| immune responses to live and inactivated nocardia seriolae and protective effect of recombinant interferon gamma (rifn γ) against nocardiosis in ginbuna crucian carp, carassius auratus langsdorfii. | looking into the fact that substantial mortality and morbidity is associated with intracellular gram +ve bacterium, nocardia seriolae infection, an effective vaccine against this pathogen is necessary to control the significant losses in aquaculture practices. therefore, an attempt was made to evaluate the effect of live (sub-lethal) and inactivated (antigenic form) n. seriolae on cellular and humoral immunity in ginbuna crucian carp, carassius auratus langsdorfii as well as the therapeutic pote ... | 2014 | 24882019 |
| [ecological risk assessment of organophosphorus pesticides in aquatic ecosystems of pearl river estuary]. | the risk quotient method and a probabilistic risk assessment method were applied for assessing aquatic ecological risk of nine organophosphorus pesticides, including thimet, dichlorovos, disulfoton, dimethoate, dimethyl parathion, chlorpyrifos, ethoprophos, sumithion and malathion on eight aquatic organisms in the pearl river estuary. results using the risk quotient method revealed that the risk level of opossum shrimp was the highest among eight aquatic organisms of the pearl river estuary. the ... | 2014 | 24881393 |
| in vitro cytotoxicity and multiplex pcr detection of virulence factors of yersinia ruckeri isolated from rainbow trout in north west germany. | the aim of this study was to investigate differences in presence and expression of virulence factors between biotype 1 and 2 strains of 82 yersinia (y.) ruckeri isolates, collected from north west germany during the period of 2004-2012, and to analyze the cytotoxicity of these strains to different fish cell lines. the common virulence factor genes, such as yhla and yhlb encoding for hemolysin yhla, rucc and rupg encoding for ruckerbactin, yrp1 and yrpdef for abc exporter protein system, and two ... | 2014 | 24881275 |
| prevalence of clonorchis sinensis infection in freshwater fishes in northeastern china. | the prevalence of clonorchis sinensis infection in freshwater fishes was surveyed in heilongjiang province, northeastern china, between august 2011 and september 2013. thirteen species of freshwater fish (n=3221) and one species of shrimp (n=93) were collected from songhua river, nenjiang river and other lakes or ponds in 37 sites of 15 representative cities in heilongjiang province. they were individually examined by digestion technique, and the c. sinensis metacercariae were identified morphol ... | 2014 | 24880648 |
| molecular responses of fishes to elevated carbon dioxide. | hypercarbia, or elevated carbon dioxide, is an environmental challenge that can have detrimental effects on the physiology and performance of aquatic organisms. with aquatic hypercarbia predicted to become more prevalent in the future due to global climate change, it is important to quantify how hypercarbia impacts aquatic organisms, especially fish. the impact of hypercarbia on the behavior and physiology of fishes has been well studied, but relatively few studies have examined the molecular pr ... | 2015 | 24875566 |
| taxonomic validity and phylogenetic relationships of a newly-described tooth-carp, aphanius mesopotamicus coad, 2009 (teleostei: cyprinodontidae). | variation among complete cytb sequences (1140 bp) of aphanius mesopotamicus coad, 2009 was compared with closely related species, to investigate the validity of this taxon as a newly-described tooth-carp based on morphological characteristics. maximum likelihood and bayesian likelihood trees supported the monophyly of a. mesopotamicus and its sister group relationship to a. sophiae. some 10-16 differences were found when compared to four different population samples of a. sophiae, whereas, intra ... | 2014 | 24871855 |
| carp is a potential tumor suppressor in gastric carcinoma and a single-nucleotide polymorphism in carp gene might increase the risk of gastric carcinoma. | the caspase-associated recruitment domain-containing protein (carp) is expressed in almost all tissues. recently, the tumor-suppressive function of carp was discovered and attracted increasing attention. this study aimed to investigate the role of carp in the carcinogenesis of human gastric carcinoma. | 2014 | 24870804 |
| two new species of the tooth-carp aphanius (teleostei: cyprinodontidae) and the evolutionary history of the iranian inland and inland-related aphanius species . | two new species of aphanius are described from the kol drainage in southern iran (aphanius darabensis n. sp.) and the endorheic kavir basin in northern iran (a. kavirensis n. sp.), and compared with eight closely related species. aphanius darabensis n. sp. is sister to a. shirini, from which it is distinguished by molecular characters (cytochrome b) and the combination of three morphological characters: 9-18 flank bars in males (vs. 7-10), females with irregular vertical patches of brown color o ... | 2014 | 24869537 |
| ph-responsive thiolated chitosan nanoparticles for oral low-molecular weight heparin delivery: in vitro and in vivo evaluation. | the aim of present study was to investigate a ph-responsive and mucoadhesive nanoparticle system for oral bioavailability enhancement of low-molecular weight heparin (lmwh). the thioglycolic acid (tga) was first covalently attached to chitosan (cs) with 396.97 ± 54.54 μmol thiol groups per gram of polymer and then the nanoparticles were prepared with thiolated chitosan (tcs) and ph-sensitive polymer hydroxypropyl methylcellulose phthalate (hpmcp) by ionic cross-linking method. the obtained nanop ... | 2016 | 24865290 |
| synthesis of (3s,3's)- and meso-stereoisomers of alloxanthin and determination of absolute configuration of alloxanthin isolated from aquatic animals. | in order to determine the absolute configuration of naturally occurring alloxanthin, a hplc analytical method for three stereoisomers 1a-c was established by using a chiral column. two authentic samples, (3s,3's)- and meso-stereoisomers 1b and 1c, were chemically synthesized according to the method previously developed for (3r,3'r)-alloxanthin (1a). application of this method to various alloxanthin specimens of aquatic animals demonstrated that those isolated from shellfishes, tunicates, and cru ... | 2014 | 24862182 |
| effects of cyhalothrin-based pesticide on early life stages of common carp (cyprinus carpio l.). | the effects of nexide (a.i. gamma-cyhalothrin 60 g l(-1)) on cumulative mortality, growth indices, and ontogenetic development of embryos and larvae of common carp (cyprinus carpio l.) were studied. levels of oxidative stress parameters glutathione reductase (gr), glutathione peroxidase (gpx), catalase (cat), glutathione-s-transferase (gst), and lipid peroxidation were determined. eggs of newly fertilised common carp were exposed to nexide at concentrations 5, 25, 50, 100, and 250 μ g l(-1) (0. ... | 2014 | 24860807 |
| lack of a contact requirement for direct antibacterial activity of lymphocyte subpopulations in ginbuna crucian carp. | cytotoxic t lymphocytes (ctl) recognize and kill cells infected with viruses, intracellular bacteria and tumors with mhc restriction and antigen specificity. in addition to these activities, recent studies in mammals have suggested that ctl can exhibit direct microbicidal activity. in our previous study we documented direct antibacterial activity of cd4(+) t cells and sigm(+) cells as well as cd8α(+) t cells from immunized fish. however, we also found weak non-specific killing activity of lympho ... | 2014 | 24859594 |
| species-specific bioaccumulation of halogenated organic pollutants and their metabolites in fish serum from an e-waste site, south china. | halogenated organic pollutants (hops)-including organochlorine pesticides (ocps), polychlorinated biphenyls (pcbs), polybrominated diphenylethers (pbdes), polybromobiphenyls (pbbs), dechlorane plus (dp), tetrabromobisphenol a (tbbpa), and hexabromocyclododecanes (hbcds) as well as pcb metabolites (methylsulfone [meso2-]) and hydroxylated (oh-) pcbs and oh-pbdes-were determined in the serum of mud carp and northern snakehead from an electronic-waste (e-waste) site in south china. the average conc ... | 2014 | 24859045 |
| rh versus ph: the role of rhesus glycoproteins in renal ammonia excretion during metabolic acidosis in a freshwater teleost fish. | increased renal ammonia excretion in response to metabolic acidosis is thought to be a conserved response in vertebrates. we tested the hypothesis that rhesus (rh) glycoproteins in the kidney of the freshwater common carp, cyprinus carpio, play a crucial role in regulating renal ammonia excretion during chronic metabolic acidosis. exposure to water ph 4.0 (72 h) resulted in a classic metabolic acidosis with reduced plasma arterial ph and [hco3(-)], no change in pco2 and large changes in renal fu ... | 2014 | 24855681 |
| effect of temperature on hypoxia tolerance and its underlying biochemical mechanism in two juvenile cyprinids exhibiting distinct hypoxia sensitivities. | it is increasingly important to investigate the effect of temperature on hypoxia tolerance in fish species, as worldwide hypoxia worsens with increases in global warming. we selected the hypoxia-tolerant crucian carp (carassius carassius) and the hypoxia-sensitive chinese bream (parabramis pekinensis) as model fish and investigated their hypoxia tolerance based on the critical oxygen tension of the routine metabolic rate (m˙o2rout) (pcrit), aquatic surface respiration (asrcrit) and loss of equil ... | 2015 | 24853206 |
| inhibition of the cardiac atp-dependent potassium current by kb-r7943. | kb-r7943 (2-[2-[4-(4-nitrobenzyloxy)phenyl]ethyl]isothiourea) was developed as a specific inhibitor of the sarcolemmal sodium-calcium exchanger (ncx) with potential experimental and therapeutic use. however, in cardiomyocytes kb-r7943 also effectively blocks several k(+) currents including the delayed rectifier, ikr, and background inward rectifier, ik1. in the present study we analyze the effects of kb-r7943 on the atp-dependent potassium current (ikatp) recorded by whole-cell patch-clamp in ve ... | 2014 | 24845199 |