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bt toxin cry1ie causes no negative effects on survival, pollen consumption, or olfactory learning in worker honey bees (hymenoptera: apidae).the honey bee (apis mellifera l.) is a key nontarget insect in environmental risk assessments of insect-resistant genetically modified crops. in controlled laboratory conditions, we evaluated the potential effects of cry1ie toxin on survival, pollen consumption, and olfactory learning of young adult honey bees. we exposed worker bees to syrup containing 20, 200, or 20,000 ng/ml cry1ie toxin, and also exposed some bees to 48 ng/ml imidacloprid as a positive control for exposure to a sublethal con ...201627122497
[the pension bee is spontaneously provided for]. 201627119681
the bee microbiome: impact on bee health and model for evolution and ecology of host-microbe interactions.as pollinators, bees are cornerstones for terrestrial ecosystem stability and key components in agricultural productivity. all animals, including bees, are associated with a diverse community of microbes, commonly referred to as the microbiome. the bee microbiome is likely to be a crucial factor affecting host health. however, with the exception of a few pathogens, the impacts of most members of the bee microbiome on host health are poorly understood. further, the evolutionary and ecological for ...201627118586
sublethal effects of acaricides and nosema ceranae infection on immune related gene expression in honeybees.nosema ceranae is an obligate intracellular parasite and the etiologic agent of nosemosis that affects honeybees. beside the stress caused by this pathogen, honeybee colonies are exposed to pesticides under beekeeper intervention, such as acaricides to control varroa mites. these compounds can accumulate at high concentrations in apicultural matrices. in this work, the effects of parasitosis/acaricide on genes involved in honeybee immunity and survival were evaluated. nurse bees were infected wi ...201627118545
linking pollen quality and performance of neoseiulus californicus (acari: phytoseiidae) in two-spotted spider mite management programmes.it has been shown that pollen as a dietary supplement may increase the establishment of generalist predatory mites, and therefore pest control by these mites can be provided. life table studies were performed to evaluate the nutritional value of seven different pollens (almond, castor-bean, date-palm, maize, bitter-orange, sunflower and mixed bee pollen) as a supplementary food source for the spider mite predator neoseiulus californicus mcgregor. in addition, the nutritional quality of each poll ...201727117203
genome sequencing and analysis of the first complete genome of lactobacillus kunkeei strain mp2, an apis mellifera gut isolate.background. the honey bee (apis mellifera) is the most important pollinator in agriculture worldwide. however, the number of honey bees has fallen significantly since 2006, becoming a huge ecological problem nowadays. the principal cause is ccd, or colony collapse disorder, characterized by the seemingly spontaneous abandonment of hives by their workers. one of the characteristics of ccd in honey bees is the alteration of the bacterial communities in their gastrointestinal tract, mainly due to t ...201627114887
the dual function of orchid bee ocelli as revealed by x-ray microtomography.visually guided flight control in the rainforest is arguably one of the most complex insect behaviors: illumination varies dramatically depending on location [1], and the densely cluttered environment blocks out most of the sky [2]. what visual information do insects sample for flight control in this habitat? to begin answering this question, we determined the visual fields of the ocelli-thought to play a role in attitude stabilization of some flying insects [3-5]-of an orchid bee, euglossa impe ...201627112298
electronic biosensing with functionalized rgo fets.in the following we give a short summary of examples for biosensor concepts in areas in which reduced graphene oxide-based electronic devices can be developed into new classes of biosensors, which are highly sensitive, label-free, disposable and cheap, with electronic signals that are easy to analyze and interpret, suitable for multiplexed operation and for remote control, compatible with nfc technology, etc., and in many cases a clear and promising alternative to optical sensors. the presented ...201627110828
therapeutic potency of bee pollen against biochemical autistic features induced through acute and sub-acute neurotoxicity of orally administered propionic acid.it is now well documented that postnatal exposure to certain chemicals has been reported to increase the risk of autism spectrum disorder. propionic acid (pa), as a metabolic product of gut microbiotaandas a commonly used food additive, has been reported to mediate the effects of autism. results from animal studies may help to identify environmental neurotoxic agents and drugs that can ameliorate neurotoxicity and may thereby aid in the treatment of autism. the present study investigated the ame ...201627107819
parasite resistance and tolerance in honeybees at the individual and social level.organisms living in large groups, such as social insects, are particularly vulnerable to parasite transmission. however, they have evolved diverse defence mechanisms which are not only restricted to the individual's immune response, but also include social defences. here, we review cases of adaptations at the individual and social level in the honeybee apis mellifera against the ectoparasitic mite varroa destructor and the endoparasitic microsporidians nosema ceranae and nosema apis. they are co ...201627106014
the dental hygiene scholarly identity and roadblocks to achieving it.progress in the dental hygiene discipline is predicated on the development of a community of scholars with a dental hygiene scholarly identity who explore questions central to the art and science of dental hygiene and in doing so create conceptual models to expand the discipline's structural hierarchy of knowledge. graduate dental hygiene education is challenged to develop programs that stimulate and nurture dental hygiene scholars as well as scientists. the need for the development of dental hy ...201627105786
dual effect of phenolic nectar on three floral visitors of elsholtzia rugulosa (lamiaceae) in sw china.some plants secrete toxic nectar to appeal to most effective pollinators and deter non-pollinators or nectar thieves; however available information about ecological function of toxic nectar remains scarce. elsholtzia rugulosa stands out as a plant with toxic nectar recorded in sw china. we focused on the functional significance of the phenolic compound that imparts toxic to the nectar of e. rugulosa. the effects of phenolic nectar were studied in three visitors of the flowers of the winter-bloom ...201627105024
effect of bee venom and its fractions on the release of pro-inflammatory cytokines in pma-differentiated u937 cells co-stimulated with lps.the venom of apis mellifera (honey bee) has been reported to play a role in immunotherapy, but existing evidence to support its immuno-modulatory claims is insufficient. four fractions from whole bee venom (bv) were separated using medium pressure liquid chromatography. their ability to induce the production of cytokines tnfα, il-1β and il-6 in phorbol-12-myristate-13-acetate (pma)-treated u937 cells was assessed. the levels of the three cytokines produced by stimulation with the four fractions ...201627104574
[acute toxicity of lds, a molluscicide, to non-target organisms].to evaluate the acute toxicity of 10% lds, a new molluscicide, to non-target organisms.201527097472
airflow and optic flow mediate antennal positioning in flying honeybees.to maintain their speeds during navigation, insects rely on feedback from their visual and mechanosensory modalities. although optic flow plays an essential role in speed determination, it is less reliable under conditions of low light or sparse landmarks. under such conditions, insects rely on feedback from antennal mechanosensors but it is not clear how these inputs combine to elicit flight-related antennal behaviours. we here show that antennal movements of the honeybee, apis mellifera, are g ...201627097104
impact of the phoretic phase on reproduction and damage caused by varroa destructor (anderson and trueman) to its host, the european honey bee (apis mellifera l.).varroa destructor is a parasitic mite of the honeybee that causes thousands of colony losses worldwide. the parasite cycle is composed of a phoretic and a reproductive phase. during the former, mites stay on adult bees, mostly on nurses, to feed on hemolymph. during the latter, the parasites enter brood cells and reproduce. we investigated if the type of bees on which varroa stays during the phoretic phase and if the duration of this stay influenced the reproductive success of the parasite and t ...201627096154
characterization of unexplored amidohydrolase enzyme-pterin deaminase.pterin deaminase is an amidohydrolase enzyme hydrolyzing pteridines to form lumazine derivatives and ammonia. the enzyme captured the attention of scientists as early as 1959 and had been patented for its application as an anticancer agent. it is ubiquitously present in prokaryotes and has been reported in some eukaryotes such as honey bee, silkworm and rats. the enzyme has been observed to have a spectrum of substrates with the formation of respective lumazines. the role of the substrates of th ...201627094187
the effects of bt cry1ie toxin on bacterial diversity in the midgut of apis mellifera ligustica (hymenoptera: apidae).the honey bee has been regarded as a key species in the environmental risk assessment of biotech crops. here, the potential adverse effects of cry1ie toxin on the midgut bacteria of the worker bees (apis mellifera ligustica) were investigated under laboratory conditions. newly emerged bees were fed with different concentrations of cry1ie toxin syrups (20 ng/ml, 200 ng/ml, and 20 μg/ml), pure sugar syrup, and 48 ppb of imidacloprid syrups, then sampled after 15 and 30 d. we characterized the domi ...201627090812
impacts of chronic sublethal exposure to clothianidin on winter honeybees.a wide application of systemic pesticides and detection of their residues in bee-collected pollen and nectar at sublethal concentrations led to the emergence of concerns about bees' chronic exposure and possible sublethal effects on insect pollinators. therefore, special attention was given to reducing unintentional intoxications under field conditions. the sensitivity of winter bees throughout their long lifespan to residual exposure of pesticides is not well known, since most previous studies ...201627090425
phospholipid-based prodrugs for drug targeting in inflammatory bowel disease: computational optimization and in-vitro correlation.in inflammatory bowel disease (ibd) patients, the enzyme phospholipase a2 (pla2) is overexpressed in the inflamed intestinal tissue, and hence may be exploited as a prodrug-activating enzyme allowing drug targeting to the site(s) of gut inflammation. the purpose of this work was to develop powerful modern computational approaches, to allow optimized a-priori design of phospholipid (pl) based prodrugs for ibd drug targeting. we performed simulations that predict the activation of pl-drug conjugat ...201627086789
honey, bee pollen and vegetable oil unsaponifiables in wound healing.we would like to remark on the mechanisms and therapeutic properties of honey, bee pollen and unsaponifiable fractions of vegetable oils in wound healing.201627085425
turkish propolis protects human endothelial cells in vitro from homocysteine-induced apoptosis.chronic cardiovascular and neurodegenerative complications induced by hyperhomocysteinemia have been most relatively associated with endothelial cell injury. elevated homocysteine (hcy) generates reactive oxygen species (ros) accompanying with oxidative stress which is hallmarks of the molecular mechanisms responsible for cardiovascular disease. propolis is a natural product, obtained by honeybee from various oils, pollens, special resins and wax materials, conventionally used with the purpose o ...201627085254
the occurrence of pesticides and persistent organic pollutants in italian organic honeys from different productive areas in relation to potential environmental pollution.bee products, such as honey, are widely consumed as food and consumer interest is currently oriented towards organic foods. regarding this, the european commission establishes that the qualification of organic honey and other beekeeping products as being from organic production is closely bound with the characteristics of hive treatments as well as the quality of the environment. agricultural contamination with pesticides is a challenging problem that needs to be fully addressed, in particular i ...201627085062
involvement of phosphorylated apis mellifera creb in gating a honeybee's behavioral response to an external stimulus.the transcription factor camp-response element-binding protein (creb) is involved in neuronal plasticity. phosphorylation activates creb and an increased level of phosphorylated creb is regarded as an indicator of creb-dependent transcriptional activation. in honeybees(apis mellifera)we recently demonstrated a particular high abundance of the phosphorylated honeybee creb homolog (pamcreb) in the central brain and in a subpopulation of mushroom body neurons. we hypothesize that these high pamcreb ...201627084927
the pollen of metaphor: box, cage, and trap as containment in the eighteenth century.this article uses the concept of "the pollen of metaphor" to discuss three forms of non-human animal containment in the eighteenth century: françois huber's leaf or book hive bee box first described in his nouvelles observations sur les abeilles (1792, english translation 1806), sarah trimmer's bird cages in her didactic children's book, fabulous histories; or, the story of the robins (1786), and a mouse trap in anna letitia barbauld's 1773 poem, "the mouse's petition, found in the trap where he ...201627084048
varroa destructor and viruses association in honey bee colonies under different climatic conditions.honey bee colonies are threatened by multiple factors including complex interactions between environmental and diseases such as parasitic mites and viruses. we compared the presence of honeybee-pathogenic viruses and varroa infestation rate in four apiaries: commercial colonies that received treatment against varroa and non-treated colonies that did not received any treatment for the last 4 years located in temperate and subtropical climate. in addition, we evaluated the effect of climate and va ...201627083139
effects of a honeybee lactic acid bacterial microbiome on human nasal symptoms, commensals, and biomarkers.lactic acid bacteria (lab) can restore commensal microbiomes and prevent infections. arguably, nasal administrations of lab may therefore be beneficial in chronic rhinosinusitis (crs). previous studies have examined effects of topical/nasal lab in children with secretory otitis media, but little is as yet known about their effects on the human nasal airway. the aim of this pilot study was to examine effects on nasal symptoms and commensal bacteria in healthy subjects of nasal administration of a ...201627080343
rising atmospheric co2 is reducing the protein concentration of a floral pollen source essential for north american bees.at present, there is substantive evidence that the nutritional content of agriculturally important food crops will decrease in response to rising levels of atmospheric carbon dioxide, ca however, whether ca-induced declines in nutritional quality are also occurring for pollinator food sources is unknown. flowering late in the season, goldenrod (solidago spp.) pollen is a widely available autumnal food source commonly acknowledged by apiarists to be essential to native bee (e.g. bombus spp.) and ...201627075256
intensively cultivated landscape and varroa mite infestation are associated with reduced honey bee nutritional state.as key pollinators, honey bees are crucial to many natural and agricultural ecosystems. an important factor in the health of honey bees is the availability of diverse floral resources. however, in many parts of the world, high-intensity agriculture could result in a reduction in honey bee forage. previous studies have investigated how the landscape surrounding honey bee hives affects some aspects of honey bee health, but to our knowledge there have been no investigations of the effects of intens ...201627070422
spiking with recombinant allergens to improve allergen extracts: benefits and limitations for the use in routine diagnostics: part 19 of the series molecular allergology.essentially, allergen components offer three possibilities to improve in vitro ige diagnostics: allergen components can be used individually for ige determination.allergen components can be combined as a mix in one test.individual allergen components can be specifically added to the extract. option (a) is currently being used most extensively in practice, while (b) represents more of a theoretical possibility. the specific addition ("spiking") of allergen components to an allergy extract (c) has ...201727069840
drug leads agents from methanol extract of nigerian bee (apis mellifera) propolis.propolis is a bee (apis mellifera) product of plant origin with varied chemical composition depending on the ecology of the botanical origin. it has been reported in literature to possess various therapeutic effects both traditionally, clinical trial, and animal study.201727069724
dna barcoding to identify leaf preference of leafcutting bees.leafcutting bees (megachile: megachilidae) cut leaves from various trees, shrubs, wildflowers and grasses to partition and encase brood cells in hollow plant stems, decaying logs or in the ground. the identification of preferred plant species via morphological characters of the leaf fragments is challenging and direct observation of bees cutting leaves from certain plant species are difficult. as such, data are poor on leaf preference of leafcutting bees. in this study, i use dna barcoding of th ...201627069650
hit-and-run trophallaxis of small hive beetles.some parasites of social insects are able to exploit the exchange of food between nestmates via trophallaxis, because they are chemically disguised as nestmates. however, a few parasites succeed in trophallactic solicitation although they are attacked by workers. the underlying mechanisms are not well understood. the small hive beetle (=shb), aethina tumida, is such a parasite of honey bee, apis mellifera, colonies and is able to induce trophallaxis. here, we investigate whether shb trophallacti ...201527069599
population structure of honey bees in the carpathian basin (hungary) confirms introgression from surrounding subspecies.carniolan honey bees (apis mellifera carnica) are considered as an indigenous subspecies in hungary adapted to most of the ecological and climatic conditions in this area. however, during the last decades hungarian beekeepers have recognized morphological signs of the italian honey bee (apis mellifera ligustica). as the natural distribution of the honey bee subspecies can be affected by the importation of honey bee queens or by natural gene flow, we aimed at determining the genetic structure and ...201527069597
design of an harmonic radar for the tracking of the asian yellow-legged hornet.the yellow-legged asian hornet is an invasive species of wasps, indigenous to the southeast asia but recently spreading in southern europe. because of its exponential diffusion and its serious threat to the local honeybee colonies (and to humans as well), restraint measures are currently under investigation. we developed and tested an harmonic radar capable of tracking the flying trajectory of these insects, once equipped with a small transponder, in their natural environment. several hornets we ...201627069583
africanization of a feral honey bee (apis mellifera) population in south texas: does a decade make a difference?the arrival to the united states of the africanized honey bee, a hybrid between european subspecies and the african subspecies apis mellifera scutellata, is a remarkable model for the study of biological invasions. this immigration has created an opportunity to study the dynamics of secondary contact of honey bee subspecies from african and european lineages in a feral population in south texas. an 11-year survey of this population (1991-2001) showed that mitochondrial haplotype frequencies chan ...201627069571
leaf fertilizers affect survival and behavior of the neotropical stingless bee friesella schrottkyi (meliponini: apidae: hymenoptera).the ongoing concern about bee decline has largely focused on honey bees and neonicotinoid insecticides, while native pollinators such as neotropical stingless bees and agrochemicals such as other insecticide groups, pesticides in general, and fertilizers-especially leaf fertilizers-remain neglected as potential contributors to pollination decline. in an effort to explore this knowledge gap, we assessed the lethal and sublethal behavioral impact of heavy metal-containing leaf fertilizers in a nat ...201627069099
host choice in a bivoltine bee: how sensory constraints shape innate foraging behaviors.many insects have multiple generations per year and cohorts emerging in different seasons may evolve their own phenotypes if they are subjected to different selection regimes. the bivoltine bee andrena bicolor is reported to be polylectic and oligolectic (on campanula) in the spring and summer generations, respectively. neurological constraints are assumed to govern pollen diet in bees. however, evidence comes predominantly from studies with oligolectic bees. we have investigated how sensory con ...201627068328
caffeic acid phenethyl ester increases radiosensitivity of estrogen receptor-positive and -negative breast cancer cells by prolonging radiation-induced dna damage.breast cancer is an important cause of death among women. the development of radioresistance in breast cancer leads to recurrence after radiotherapy. caffeic acid phenethyl ester (cape), a polyphenolic compound of honeybee propolis, is known to have anticancer properties. in this study, we examined whether cape enhanced the radiation sensitivity of mda-mb-231 (estrogen receptor-negative) and t47d (estrogen receptor-positive) cell lines.201627066092
specific immune stimulation by endogenous bacteria in honey bees (hymenoptera: apidae).honey bees are highly important pollinators in agroecosystems, but they are currently under growing environmental pressures (e.g., from pesticides, poor nutrition, and parasites). due to the multiplicity of environmental stress factors, their protection requires diverse and integrative approaches. among those is the development of immunomodulatory tools, as immunosuppression is often observed in stressed bees. toward this goal, the use of exogenous bacteria with immunomodulatory potential has re ...201627063842
effect of honey on febrile neutropenia in children with acute lymphoblastic leukemia: a randomized crossover open-labeled study.febrile neutropenia (fn) is a common adverse effect of chemotherapy. current management of fn is expensive and may induce side effects. honey, as a natural honeybee product, has antioxidant, antimicrobial, immunomodulator and anticancer effects. additionally, honey is not expensive. the aim of this study is to test the effects of a 12-week honey consumption on children with acute lymphoblastic leukemia (all) particularly with regard to fn.201627062956
first reported case of fatal stinging by the large carpenter bee xylocopa tranquebarica.in the order hymenoptera, bees, hornets, and wasps are well-known stinging insects whose envenoming can be fatal. their stinging attacks are common in rural and forested areas of sri lanka. however, fatal stinging by the large-bodied carpenter bees is unreported. we report the first known case of a fatal sting by the large carpenter bee, xylocopa tranquebarica, in a forested area in puttalam (north western province) in the dry zone of sri lanka. a 59-year-old healthy male manual laborer accompan ...201627061039
an experimental platform using human intestinal epithelial cell lines to differentiate between hazardous and non-hazardous proteins.human intestinal epithelial cell lines (t84, caco-2, and hct-8) grown on permeable transwell™ filters serve as models of the gastrointestinal barrier. in this study, this in vitro model system was evaluated for effectiveness at distinguishing between hazardous and non-hazardous proteins. indicators of cytotoxicity (ldh release, mtt conversion), monolayer barrier integrity ([(3)h]-inulin flux, horseradish peroxidase flux, trans-epithelial electrical resistance [teer]), and inflammation (il-8, il- ...201627060235
evolution of the microstructure of unmodified and polymer modified asphalt binders with aging in an accelerated weathering tester.this paper presents findings on the evolution of the surface microstructure of two asphalt binders, one unmodified and one polymer modified, directly exposed to aging agents with increasing durations. the aging is performed using an accelerated weathering tester, where ultraviolet radiation, oxygen and an increased temperature are applied to the asphalt binder surface. ultraviolet and dark cycles, which simulated the succession of day and night, alternated during the aging process, and also the ...201627059404
mitochondrial structure and dynamics as critical factors in honey bee (apis mellifera l.) caste development.the relationship between nutrition and phenotype is an especially challenging question in cases of facultative polyphenism, like the castes of social insects. in the honey bee, apis mellifera, unexpected modifications in conserved signaling pathways revealed the hypoxia response as a possible mechanism underlying the regulation of body size and organ growth. hence, the current study was designed to investigate possible causes of why the three hypoxia core genes are overexpressed in worker larvae ...201627058771
exploring the ontogenetic scaling hypothesis during the diversification of pollination syndromes in caiophora (loasaceae, subfam. loasoideae).phenotypic diversification of flowers is frequently attributed to selection by different functional groups of pollinators. during optimization of floral phenotype, developmental robustness to genetic and non-genetic perturbations is expected to limit the phenotypic space available for future evolutionary changes. although adaptive divergence can occur without altering the basic developmental programme of the flower (ontogenetic scaling hypothesis), the rarity of reversion to ancestral states fol ...201627056974
safety and tolerability during build-up phase of a rush venom immunotherapy.safety and tolerability of venom immunotherapy (vit) in patients with concomitant disease and comedications, especially β-blockers (bbs) and angiotensin-converting enzyme inhibitors (aceis), are under discussion.201627055991
role of the cationic c-terminal segment of melittin on membrane fragmentation.the widespread distribution of cationic antimicrobial peptides capable of membrane fragmentation in nature underlines their importance to living organisms. in the present work, we determined the impact of the electrostatic interactions associated with the cationic c-terminal segment of melittin, a 26-amino acid peptide from bee venom (net charge +6), on its binding to model membranes and on the resulting fragmentation. in order to detail the role played by the c-terminal charges, we prepared a m ...201627054924
pathogen prevalence and abundance in honey bee colonies involved in almond pollination.honey bees are important pollinators of agricultural crops. since 2006, us beekeepers have experienced high annual honey bee colony losses, which may be attributed to multiple abiotic and biotic factors, including pathogens. however, the relative importance of these factors has not been fully elucidated. to identify the most prevalent pathogens and investigate the relationship between colony strength and health, we assessed pathogen occurrence, prevalence, and abundance in western us honey bee c ...201727053820
finding the gap: a brightness-based strategy for guidance in cluttered environments.the ability to move safely between obstacles is critical for animals that fly rapidly through cluttered environments but surprisingly little is known about how they achieve this. do they reactively avoid obstacles or do they instead fly towards the gaps between them? if they aim towards gaps, what information do they use to detect and fly through them? here, we aim to answer these questions by presenting orchid bees with different apertures. when negotiating gaps, orchid bees locate and fly clos ...201627053748
chronic neonicotinoid pesticide exposure and parasite stress differentially affects learning in honeybees and bumblebees.learning and memory are crucial functions which enable insect pollinators to efficiently locate and extract floral rewards. exposure to pesticides or infection by parasites may cause subtle but ecologically important changes in cognitive functions of pollinators. the potential interactive effects of these stressors on learning and memory have not yet been explored. furthermore, sensitivity to stressors may differ between species, but few studies have compared responses in different species. here ...201627053744
myocardial infarction following a bee sting: a case report of kounis syndrome.in this case report study a 41-year-old man envenomed by a bee sting and diagnosed as kounis syndrome secondary to hymenoptera envenomation. the patient developed a typical course of myocardial infarction, but the electrocardiogram changes were reversed to almost normal limits. he had a nonsignificant mild lesion in the proximal port of right coronary artery in coronary angiography. the case recovered and discharged after 6 days hospitalization. the clinical implications and pathophysiology of t ...201727052090
the genome and methylome of a subsocial small carpenter bee, ceratina calcarata.understanding the evolution of animal societies, considered to be a major transition in evolution, is a key topic in evolutionary biology. recently, new gateways for understanding social evolution have opened up due to advances in genomics, allowing for unprecedented opportunities in studying social behavior on a molecular level. in particular, highly eusocial insect species (caste-containing societies with nonreproductives that care for siblings) have taken center stage in studies of the molecu ...201627048475
enhancing legume ecosystem services through an understanding of plant-pollinator interplay.legumes are bee-pollinated, but to a different extent. the importance of the plant-pollinator interplay (ppi), in flowering crops such as legumes lies in a combination of the importance of pollination for the production service and breeding strategies, plus the increasing urgency in mitigating the decline of pollinators through the development and implementation of conservation measures. to realize the full potential of the ppi, a multidisciplinary approach is required. this article assembles an ...201627047514
test of the invasive pathogen hypothesis of bumble bee decline in north america.emergent fungal diseases are critical factors in global biodiversity declines. the fungal pathogennosema bombiwas recently found to be widespread in declining species of north american bumble bees (bombus), with circumstantial evidence suggesting an exotic introduction from europe. this interpretation has been hampered by a lack of knowledge of global genetic variation, geographic origin, and changing prevalence patterns ofn. bombiin declining north american populations. thus, the temporal and s ...201627044096
bee venom promotes hair growth in association with inhibiting 5α-reductase expression.alopecia is an important issue that can occur in people of all ages. recent studies show that bee venom can be used to treat certain diseases including rheumatoid arthritis, neuralgia, and multiple sclerosis. in this study, we investigated the preventive effect of bee venom on alopecia, which was measured by applying bee venom (0.001, 0.005, 0.01%) or minoxidil (2%) as a positive control to the dorsal skin of female c57bl/6 mice for 19 d. growth factors responsible for hair growth were analyzed ...201627040904
landscape and local correlates of bee abundance and species richness in urban gardens.urban gardens may preserve biodiversity as urban population densities increase, but this strongly depends on the characteristics of the gardens and the landscapes in which they are embedded. we investigated whether local and landscape characteristics are important correlates of bee (hymenoptera: apiformes) abundance and species richness in urban community gardens. we worked in 19 gardens in the california central coast and sampled bees with aerial nets and pan traps. we measured local characteri ...201627034445
pathological evidence of foreign body reaction induced by bee sting therapy. 201627031826
larval starvation improves metabolic response to adult starvation in honey bees (apis mellifera l.).environmental changes during development have long-term effects on adult phenotypes in diverse organisms. some of the effects play important roles in helping organisms adapt to different environments, such as insect polymorphism. others, especially those resulting from an adverse developmental environment, have a negative effect on adult health and fitness. however, recent studies have shown that those phenotypes influenced by early environmental adversity have adaptive value under certain (anti ...201627030776
starvation stress during larval development facilitates an adaptive response in adult worker honey bees (apis mellifera l.).most organisms are constantly faced with environmental changes and stressors. in diverse organisms, there is an anticipatory mechanism during development that can program adult phenotypes. the adult phenotype would be adapted to the predicted environment that occurred during organism maturation. however, whether this anticipatory mechanism is present in eusocial species is questionable because eusocial organisms are largely shielded from exogenous conditions by their stable nest environment. in ...201627030775
linking measures of colony and individual honey bee health to survival among apiaries exposed to varying agricultural land use.we previously characterized and quantified the influence of land use on survival and productivity of colonies positioned in six apiaries and found that colonies in apiaries surrounded by more land in uncultivated forage experienced greater annual survival, and generally more honey production. here, detailed metrics of honey bee health were assessed over three years in colonies positioned in the same six apiaries. the colonies were located in north dakota during the summer months and were transpo ...201627027871
existence of paenibacillus larvae genotypes eric i-st2, eric i-st15 and eric ii-st10 in the western region of aichi prefecture, japan.american foulbrood is the most destructive honeybee bacterial disease. the etiological agent, paenibacillus larvae, has been classified into four genotypes by a repetitive-element pcr (eric i-iv) and 21 sequence types by multilocus sequence typing (st1-21). in this study, we genotyped japanese p. larvae isolates for the first time and revealed the presence of three genotypes (eric i-st2, eric i-st15 and eric ii-st10) in the western region of aichi prefecture. eric i-st15 and eric ii-st10 are glo ...201627020320
the full moon as a synchronizer of circa-monthly biological rhythms: chronobiologic perspectives based on multidisciplinary naturalistic research.biological rhythmicity is presumed to be an advantageous genetic adaptation of fitness and survival value resulting from evolution of life forms in an environment that varies predictably-in-time during the 24 h, month, and year. the 24 h light/dark cycle is the prime synchronizer of circadian periodicities, and its modulation over the course of the year, in terms of daytime photoperiod length, is a prime synchronizer of circannual periodicities. circadian and circannual rhythms have been the maj ...201727019304
optimizing drone fertility with spring nutritional supplements to honey bee (hymenoptera: apidae) colonies.supplemental feeding of honey bee (apis melliferal., hymenoptera: apidae) colonies in spring is essential for colony buildup in northern apicultural regions. the impact of pollen and syrup feeding on drone production and sperm quality is not well-documented, but may improve fecundation of early-bred queens. we measured the impact of feeding sucrose syrup, and protein supplements to colonies in early spring in eastern canada. drones were reared under different nutritional regimes, and mature indi ...201627018435
floral colours in a world without birds and bees: the plants of macquarie island.we studied biotically pollinated angiosperms on macquarie island, a remote site in the southern ocean with a predominately or exclusively dipteran pollinator fauna, in an effort to understand how flower colour affects community assembly. we compared a distinctive group of cream-green macquarie island flowers to the flora of likely source pools of immigrants and to a continental flora from a high latitude in the northern hemisphere. we used both dipteran and hymenopteran colour models and phyloge ...201627016399
immune thrombocytopenia after bee venom therapy: a case report.immune thrombocytopenia (itp) is a hematological disorder with an isolated decrease in number of circulating platelets. bee venom therapy (bvt) is a form of alternative medicine. it is still being practiced in the middle east and other parts of asia. in bvt, acupuncture points are used to inject diluted bee venom into the body. the pharmacological basis behind bvt is not fully understood. however, it has been used to treat various medical conditions such as arthritis and low back pain. on the ot ...201627015661
honey bee inhibitory signaling is tuned to threat severity and can act as a colony alarm signal.alarm communication is a key adaptation that helps social groups resist predation and rally defenses. in asia, the world's largest hornet, vespa mandarinia, and the smaller hornet, vespa velutina, prey upon foragers and nests of the asian honey bee, apis cerana. we attacked foragers and colony nest entrances with these predators and provide the first evidence, in social insects, of an alarm signal that encodes graded danger and attack context. we show that, like apis mellifera, a. cerana possess ...201627014876
intraspecific variability of floral nectar volume and composition in rapeseed (brassica napus l. var. oleifera).numerous angiosperms rely on pollinators to ensure efficient flower fertilization, offering a reward consisting of nourishing nectars produced by specialized floral cells, known as nectaries. nectar components are believed to derive from phloem sap that is enzymatically processed and transformed within nectaries. an increasing body of evidence suggests that nectar composition, mainly amino acids, may influence pollinator attraction and fidelity. this notwithstanding, little is known about the ra ...201627014311
biological and therapeutic properties of bee pollen: a review.natural products, including bee products, are particularly appreciated by consumers and are used for therapeutic purposes as alternative drugs. however, it is not known whether treatments with bee products are safe and how to minimise the health risks of such products. among others, bee pollen is a natural honeybee product promoted as a valuable source of nourishing substances and energy. the health-enhancing value of bee pollen is expected due to the wide range of secondary plant metabolites (t ...201627013064
whole-genome sequence of bacillus sp. sdli1, isolated from the social bee scaptotrigona depilis.we announce the complete genome sequence ofbacillussp. strain sdli1, isolated from larval gut of the stingless beescaptotrigona depilis the 4.13-mb circular chromosome harbors biosynthetic gene clusters for the production of antimicrobial compounds.201627013050
safety of ultrarush venom immunotherapy: comparison between children and adults.the ultrarush protocol is an attractive approach in the buildup phase of venom immunotherapy (vit-ur). however, the degree of risk of vit-ur in children remains unknown. the objective of this study was to compare the safety of vit-ur in children and adults.201627012015
sdf1-cxcr4 signaling contributes to the transition from acute to chronic pain state.emerging evidence has demonstrated the involvement of stromal cell-derived factor 1 (sdf1, also known as cxcl12)-cxcr4 signaling in a variety of pain state. however, the underlying mechanisms of sdf1-cxcr4 signaling leading to the maintenance of chronic pain states are poorly understood. in the present study, we sought to explore the role of sdf1-cxcr4 signaling in the forming of neuroplasticity by applying a model of the transition from acute to chronic pain state, named as hyperalgesic priming ...201627011380
laboratory bioassays on the impact of cadmium, copper and lead on the development and survival of honeybee (apis mellifera l.) larvae and foragers.honeybees (apis mellifera l.) have been widely distributed around the world to serve as pollinators for agriculture. they can encounter metal pollutants through various routes of exposure, including foraging on contaminated plant resources. chronic and acute toxicity tests were conducted on larvae using artificial diets and on foragers using solutions of 50% sucrose, which contained cadmium (cd), copper (cu) and lead (pb). we found that mortality increased in both larvae and foragers in a dose-d ...201627011322
development of wax-incorporated emulsion gel beads for the encapsulation and intragastric floating delivery of the active antioxidant from tamarindus indica l.in this study, tamarind (tamarindus indica l.) seed extracts with potential antioxidant activity and toxicity to cancer cells were developed as functional foods and nutraceutical ingredients in the form of emulsion gel beads. three extracts were obtained from ethanol and water: tsch50, tsch95 and tsch. all extracts exhibited high potential for superoxide anion scavenging activity over the ic50 range < 5-11 µg/ml and had no toxic effects on normal cells, however, the water extract (tsch) was the ...201627011162
evaluation of apis mellifera syriaca levant region honeybee conservation using comparative genome hybridization.apis mellifera syriaca is the native honeybee subspecies of jordan and much of the levant region. it expresses behavioral adaptations to a regional climate with very high temperatures, nectar dearth in summer, attacks of the oriental wasp and is resistant to varroa mites. the a. m. syriaca control reference sample (crs) in this study was originally collected and stored since 2001 from "wadi ben hammad", a remote valley in the southern region of jordan. morphometric and mitochondrial dna markers ...201627010806
self-powered analogue smart skin.the progress of smart skin technology presents unprecedented opportunities for artificial intelligence. resolution enhancement and energy conservation are critical to improve the perception and standby time of robots. here, we present a self-powered analogue smart skin for detecting contact location and velocity of the object, based on a single-electrode contact electrification effect and planar electrostatic induction. using an analogue localizing method, the resolution of this two-dimensional ...201627010713
occurrence of moulds from bee pollen in central italy--a preliminary study.the present study aimed to preliminary evaluate the occurrence of fungi in 40 specimens of trapped pollen collected from april-september 2015 in 40 apiaries from tuscany (central italy). cultural and microscopical examinations allowed the recognition of cladosporium sp., alternaria sp., humicola sp. mucoraceae and acremonium sp. penicillium brevicompactum, penicillium chrysogenum, aspergillus flavus , aspergillus nidulans, aspergillus niger and aspergillus terreus were also identified. yeasts an ...201627010410
immune and clinical response to honeybee venom in beekeepers.the aim of the study was to assess immune response to honeybee venom in relation to the degree of exposure, time after a sting and clinical symptoms.201627007529
could caffeic acid phenethyl ester expand the antitumor effect of tamoxifen in breast carcinoma?despite tamoxifen (tam) is beneficial in treating a significant proportion of patients with breast cancer, many women still relapse after long-term therapy. caffeic acid phenethyl ester (cape) is a component of honeybee propolis, with a plethora of important biological actions including anticancer activity. this study aimed to explore the cytotoxicity, the type of drugs interaction as well as the apoptotic and autophagic pathways of the combined treatment of tam and cape in mcf-7 cells. their an ...201627007181
determination of alkaloids in onion nectar by micellar electrokinetic chromatography.nectar is the most important floral reward offered by plants to insects. minor components such as alkaloid compounds in nectar affect bee foraging, with great influence in seed production. ce is an advantageous tool for the analysis of unexplored samples such as onion nectar due to the limited amounts of samples. considering the importance of these compounds, a simultaneous determination of nicotine, theophylline, theobromine, caffeine, harmaline, piperine in onion nectar by mekc-uv is herein re ...201627005835
magnetic sensing through the abdomen of the honey bee.honey bees have the ability to detect the earth's magnetic field, and the suspected magnetoreceptors are the iron granules in the abdomens of the bees. to identify the sensing route of honey bee magnetoreception, we conducted a classical conditioning experiment in which the responses of the proboscis extension reflex (per) were monitored. honey bees were successfully trained to associate the magnetic stimulus with a sucrose reward after two days of training. when the neural connection of the ven ...201627005398
gigantobilharzia, possible cause of cercarial dermatitis: case report.cercarial dermatitis (swimmer's itch) is a worldwide, often neglected parasitic skin disease characterized by strong maculopapular skin eruption accompanied by intensive itching. a fisherman suffered from forearm dermatitis. clinical history associated with the recovery of the avian schistosome; gigantobilharzia from little green bee-eater (merops orientalis najdanus) and collected lymnaea snails supported the authors' opinion that patient clinical signs are most likely due to the invasion of av ...201627004065
synergistic effects induced by combined treatments of aqueous extract of propolis and venom.breast cancer is a heterogeneous disease and the leading cause of cancer mortality worldwide. triple negative breast cancer (tnbc) is considered to be one of the most aggressive breast neoplasia due to failure of chemotherapy response. thus, there is an urgent need of finding alternative therapies for tnbc. this study was designed to evaluate the synergistic effect induced by propolis and bee venom on luminal (mcf-7) and tnbc (hs578t) cell lines.201627004032
a new family of insect muscarinic acetylcholine receptors.most currently used insecticides are neurotoxic chemicals that target a limited number of sites and insect cholinergic neurotransmission is the major target. a potential target for insecticide development is the muscarinic acetylcholine receptor (machr), which is a metabotropic g-protein-coupled receptor. insects have a- and b-type machrs and the five mammalian machrs are close to the a-type. we isolated a cdna (cg12796) from the fruit fly, drosophila melanogaster. after heterologous expression ...201627003873
a petrinet-based approach for supporting traceability in cyber-physical manufacturing systems.with the growing popularity of complex dynamic activities in manufacturing processes, traceability of the entire life of every product has drawn significant attention especially for food, clinical materials, and similar items. this paper studies the traceability issue in cyber-physical manufacturing systems from a theoretical viewpoint. petri net models are generalized for formulating dynamic manufacturing processes, based on which a detailed approach for enabling traceability analysis is presen ...201626999141
antimicrobial peptide from the wild bee hylaeus signatus venom and its analogues: structure-activity study and synergistic effect with antibiotics.venoms of hymenopteran insects have attracted considerable interest as a source of cationic antimicrobial peptides (amps). in the venom of the solitary bee hylaeus signatus (hymenoptera: colletidae), we identified a new hexadecapeptide of sequence gly-ile-met-ser-ser-leu-met-lys-lys-leu-ala-ala-his-ile-ala-lys-nh2. named hyl, it belongs to the category of α-helical amphipathic amps. hyl exhibited weak antimicrobial activity against several strains of pathogenic bacteria and moderate activity aga ...201626998557
systemic immediate hypersensitive reactions after treatment with sweet bee venom: a case report.a previous study showed that bee venom (bv) could cause anaphylaxis or other hypersensitivity reactions. although hypersensitivity reactions due to sweet bee venom (sbv) have been reported, sbv has been reported to be associated with significantly reduced sensitization compared to bv. although no systemic immediate hypersensitive response accompanied by abnormal vital signs has been reported with respect to sbv, we report a systemic immediate hypersensitive response that we experienced while try ...201526998391
honey bee venom (apis mellifera) contains anticoagulation factors and increases the blood-clotting time.bee venom (bv) is a complex mixture of proteins and contains proteins such as phospholipase and melittin, which have an effect on blood clotting and blood clots. the mechanism of action of honey bee venom (hbv, apis mellifera) on human plasma proteins and its anti-thrombotic effect were studied. the purpose of this study was to investigate the anti-coagulation effect of bv and its effects on blood coagulation and purification.201526998384
variable effects of nicotine, anabasine, and their interactions on parasitized bumble bees.secondary metabolites in floral nectar have been shown to reduce parasite load in two common bumble bee species. previous studies on the effects of nectar secondary metabolites on parasitized bees have focused on single compounds in isolation; however, in nature, bees are simultaneously exposed to multiple compounds. we tested for interactions between the effects of two alkaloids found in the nectar of nicotiana spp. plants, nicotine and anabasine, on parasite load and mortality in bumble bees ( ...201526998225
population genetic structure and long-distance dispersal of a recently expanding migratory bird.long-distance dispersal events and their derivable increases of genetic diversity have been highlighted as important ecological and evolutionary determinants that improve performances of range-expanding species. in the context of global environmental change, specific dispersal strategies have to be understood and foreseen if we like to prevent general biodiversity impoverishment or the spread of allochthonous diseases. we explored the genetic structure and potential population mixing on the rece ...201626994943
phenophysiological variation of a bee that regulates hive humidity, but not hive temperature.seasonal acclimatisation of thermal tolerance, evaporative water loss and metabolic rate, along with regulation of the hive environment, are key ways whereby hive-based social insects mediate climatic challenges throughout the year, but the relative importance of these traits remains poorly understood. here, we examined seasonal variation in metabolic rate and evaporative water loss of worker bees, and seasonal variation of hive temperature and relative humidity (rh), for the stingless bee austr ...201626994173
optimizing fluctuating thermal regime storage of developing megachile rotundata (hymenoptera: megachilidae).the alfalfa leafcutting bee, megachile rotundata (f.), is the primary pollinator for alfalfa seed production in north america. under current management practice, developing pupae are incubated at 29-30°c until the adults emerge for pollination. if unfavorable spring weather delays peak alfalfa bloom, managers will cool pupae to slow development, which can increase mortality and causes sublethal effects. previously, we demonstrated that exposure to a fluctuating thermal regime (ftr) increases sur ...201626994099
stinging insect identification: are the allergy specialists any better than their patients?it has been reported that the general population is not skillful at identifying stinging insects with the exception of the honeybee. no information is available to evaluate allergy physicians' accuracy with stinging insect identification.201626993171
larva-mediated chalkbrood resistance-associated single nucleotide polymorphism markers in the honey bee apis mellifera.chalkbrood is a disease affecting honey bees that seriously impairs brood growth and productivity of diseased colonies. although honey bees can develop chalkbrood resistance naturally, the details underlying the mechanisms of resistance are not fully understood, and no easy method is currently available for selecting and breeding resistant bees. finding the genes involved in the development of resistance and identifying single nucleotide polymorphisms (snps) that can be used as molecular markers ...201626991518
molecular effects of neonicotinoids in honey bees (apis mellifera).neonicotinoids are implicated in the decline of bee populations. as agonists of nicotinic acetylcholine receptors, they disturb acetylcholine receptor signaling leading to neurotoxicity. several behavioral studies showed the link between neonicotinoid exposure and adverse effects on foraging activity and reproduction. however, molecular effects underlying these effects are poorly understood. here we elucidated molecular effects at environmental realistic levels of three neonicotinoids and nicoti ...201626990785
effects of imidacloprid and varroa destructor on survival and health of european honey bees, apis mellifera.there has been growing concern over declines in populations of honey bees and other pollinators which are a vital part to our food security. it is imperative to identify factors responsible for accelerated declines in bee populations and develop solutions for reversing bee losses. while exact causes of colony losses remain elusive, risk factors thought to play key roles are ectoparasitic mites varroa destructor and neonicotinoid pesticides. the present study aims to investigate effects of a neon ...201626990560
biological properties and therapeutic applications of propolis.propolis is a resinous material collected by bees from bud and exudates of the plants, mixed with bee enzymes, pollen and wax. in this review, the biological properties of propolis and some therapeutic applications are discussed. the same biological activities have been investigated until today, using samples from different geographic regions. thus, the study of the biological properties of a given sample should always be associated with its chemical composition and botanical source, representin ...201626988443
phylogenetic systematics and a revised generic classification of anthidiine bees (hymenoptera: megachilidae).the bee tribe anthidiini (hymenoptera: megachilidae) is a large, cosmopolitan group of solitary bees that exhibit intriguing nesting behavior. we present the first molecular-based phylogenetic analysis of relationships within anthidiini using model-based methods and a large, multi-locus dataset (five nuclear genes, 5081 base pairs), as well as a combined analysis using our molecular dataset in conjunction with a previously published morphological matrix. we discuss the evolution of nesting behav ...201626988413
how methylglyoxal kills bacteria: an ultrastructural study.antibacterial activity of honey is due to the presence of methylglyoxal (mgo), h2o2, bee defensin as well as polyphenols. high mgo levels in manuka honey are the main source of antibacterial activity. manuka honey has been reported to reduce the swarming and swimming motility of pseudomonas aeruginosa due to de-flagellation. due to the complexity of honey it is unknown if this effect is directly due to mgo. in this ultrastructural investigation the effects of mgo on the morphology of bacteria an ...201626986806
the allometry of bee proboscis length and its uses in ecology.allometric relationships among morphological traits underlie important patterns in ecology. these relationships are often phylogenetically shared; thus quantifying allometric relationships may allow for estimating difficult-to-measure traits across species. one such trait, proboscis length in bees, is assumed to be important in structuring bee communities and plant-pollinator networks. however, it is difficult to measure and thus rarely included in ecological analyses. we measured intertegular d ...201626986000
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