Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| kissing bug (triatoma spp.) intrusion into homes: troublesome bites and domiciliation. | kissing bugs (triatoma spp.) frequently enter homes and bite human and pet occupants. bites may lead to severe allergic reactions and, in some cases, death. kissing bugs are also vectors of trypanosoma cruzi, the cause of chagas disease. in general, modern houses in the united states are not conducive to domiciliation of kissing bugs (bugs living out their entire life within the home with the presence of eggs, nymphs, adults, and exuviae). construction features such as concrete foundations, soli ... | 2016 | 27042091 |
| american tegumentary leishmaniasis: t-cell differentiation profile of cutaneous and mucosal forms-co-infection with trypanosoma cruzi. | american tegumentary leishmaniasis displays two main clinical forms: cutaneous (cl) and mucosal (ml). ml is more resistant to treatment and displays a more severe and longer evolution. since both forms are caused by the same leishmania species, the immunological response of the host may be an important factor determining the evolution of the disease. herein, we analyzed the differentiation and memory profile of peripheral cd4(+) and cd8(+) t lymphocytes of patients with cl and ml and their leish ... | 2016 | 27040974 |
| modulation of inflammatory and oxidative status by exercise attenuates cardiac morphofunctional remodeling in experimental chagas cardiomyopathy. | the rational basis that explains the benefits of exercise therapy on chagas cardiomyopathy (chc) is poorly understood. this study investigated the impact of an exercise program on exercise performance, heart parasitism, immunoinflammatory response, fibrogenesis, oxidative damage, and cardiomyocytes contractility in experimental chc. | 2016 | 27040670 |
| analysis of the transmission of trypanosoma cruzi infection through hosts and vectors. | calculating epidemiological measures of infection by trypanosoma cruzi, the causative agent of chagas disease, is complex, because it involves several species, different stages of infection in humans and multiple transmission routes. using the next-generation matrix method, we analysed a model which considers the three stages of human infection, triatomines and dogs (the main domestic reservoirs of t. cruzi when triatomines are present) and the main transmission routes. we derived r 0 and type-r ... | 2016 | 27039662 |
| host cell invasion and oral infection by trypanosoma cruzi strains of genetic groups tci and tciv from chagasic patients. | outbreaks of acute chagas disease by oral infection have been reported frequently over the last ten years, with higher incidence in northern south america, where trypanosoma cruzi lineage tci predominates, being responsible for the major cause of resurgent human disease, and a small percentage is identified as tciv. mechanisms of oral infection and host-cell invasion by these parasites are poorly understood. to address that question, we analyzed t. cruzi strains isolated from chagasic patients i ... | 2016 | 27038796 |
| chagas disease. | 2016 | 27037106 | |
| how trypanosoma cruzi deals with oxidative stress: antioxidant defence and dna repair pathways. | trypanosoma cruzi, the causative agent of chagas disease, is an obligatory intracellular parasite with a digenetic life cycle. due to the variety of host environments, it faces several sources of oxidative stress. in addition to reactive oxygen species (ros) produced by its own metabolism, t. cruzi must deal with high ros levels generated as part of the host's immune responses. hence, the conclusion that t. cruzi has limited ability to deal with ros (based on the lack of a few enzymes involved w ... | 2016 | 27036062 |
| trypanosoma cruzi needs a signal provided by reactive oxygen species to infect macrophages. | during trypanosoma cruzi infection, macrophages produce reactive oxygen species (ros) in a process called respiratory burst. several works have aimed to elucidate the role of ros during t. cruzi infection and the results obtained are sometimes contradictory. t. cruzi has a highly efficiently regulated antioxidant machinery to deal with the oxidative burst, but the parasite macromolecules, particularly dna, may still suffer oxidative damage. guanine (g) is the most vulnerable base and its oxidati ... | 2016 | 27035573 |
| development of a novel multiplex immunoassay multi-cruzi for the serological confirmation of chagas disease. | chagas disease is due to the parasite trypanosoma cruzi, a protist disseminated by a triatome vector. this disease is endemic to latin america and considered by who as one of the 17 world's neglected diseases. in europe and in north america, imported cases are also detected, due to migration of population outside of the endemic region. diagnosis of t. cruzi infection is usually made indirectly by the detection of specific antibodies to t. cruzi antigens. following initial diagnostic evaluation o ... | 2016 | 27035146 |
| il18 gene variants influence the susceptibility to chagas disease. | chagas disease is a parasitic disorder caused by the infection with the flagellated protozoan trypanosoma cruzi. according to the world health organization, more than six million people are currently infected in endemic regions. genetic factors have been proposed to influence predisposition to infection and development of severe clinical phenotypes like chronic chagas cardiomyopathy (ccc). interleukin 18 (il18) encodes a proinflammatory cytokine that has been proposed to be involved in controlli ... | 2016 | 27027876 |
| marking triatoma brasiliensis, triatoma pseudomaculata and rhodnius nasutus nymphs with trace elements: element persistence and effects of marking on insect mortality. | field ecologists often rely on mark-release-recapture (mrr) experiments to estimate population dynamics parameters for a given species. in the case of a medically important taxon, i.e., a disease vector, inferences on species survival and dispersal rates are particularly important as they have the potential to provide insights into disease transmission dynamics in endemic areas. medical entomologists have traditionally used fluorescent dusts to externally mark the cuticle of insects. however, du ... | 2016 | 27027503 |
| status of vaccine research and development of vaccines for chagas disease. | 2016 | 27026146 | |
| antichagasic and trichomonacidal activity of 1-substituted 2-benzyl-5-nitroindazolin-3-ones and 3-alkoxy-2-benzyl-5-nitro-2h-indazoles. | two series of new 5-nitroindazole derivatives, 1-substituted 2-benzylindazolin-3-ones (6-29, series a) and 3-alkoxy-2-benzyl-2h-indazoles (30-37, series b), containing differently functionalized chains at position 1 and 3, respectively, have been synthesized starting from 2-benzyl-5-nitroindazolin-3-one 5, and evaluated against the protozoan parasites trypanosoma cruzi and trichomonas vaginalis, etiological agents of chagas disease and trichomonosis, respectively. many indazolinones of series a ... | 2016 | 27017556 |
| value of cardiac mri for evaluation of chronic chagas disease cardiomyopathy. | to determine whether cardiac magnetic resonance imaging (cmri) is more sensitive than electrocardiogram (ecg) and echocardiogram (echo) for detecting myocardial involvement in a latin american migrant population with untreated chagas disease (cd) in the united states. | 2016 | 27017480 |
| addressing the neglect: chagas disease in london, uk. | 2016 | 27013306 | |
| survival and immune response of the chagas vector meccus pallidipennis (hemiptera: reduviidae) against two entomopathogenic fungi, metarhizium anisopliae and isaria fumosorosea. | chagas disease is a key health problem in latin america and is caused and transmitted by trypanosoma cruzi and triatomine bugs, respectively. control of triatomines has largely relied on the use pyrethroids, which has proved to be ineffective in the long term. alternatively, the use of entomopathogenic fungi has been implemented to control triatomine bugs. these fungi are highly efficient as they induce a reduction in immune response on insects. meccus pallidipennis is the main triatomine vector ... | 2016 | 27012246 |
| transient superdiffusion and long-range correlations in the motility patterns of trypanosomatid flagellate protozoa. | we report on a diffusive analysis of the motion of flagellate protozoa species. these parasites are the etiological agents of neglected tropical diseases: leishmaniasis caused by leishmania amazonensis and leishmania braziliensis, african sleeping sickness caused by trypanosoma brucei, and chagas disease caused by trypanosoma cruzi. by tracking the positions of these parasites and evaluating the variance related to the radial positions, we find that their motions are characterized by a short-tim ... | 2016 | 27007779 |
| overexpression of bromodomain factor 3 in trypanosoma cruzi (tcbdf3) affects differentiation of the parasite and protects it against bromodomain inhibitors. | the bromodomain is the only protein domain known to bind acetylated lysine. in the last few years many bromodomain inhibitors have been developed in order to treat diseases such as cancer caused by aberrant acetylation of lysine residues. we have previously characterized trypanosoma cruzi bromodomain factor 3 (tcbdf3), a bromodomain with an atypical localization that binds acetylated α-tubulin. in the present work we show that parasites overexpressing tcbdf3 exhibit altered differentiation patte ... | 2016 | 27007774 |
| kinetic and molecular characterization of the pyruvate phosphate dikinase from trypanosoma cruzi. | trypanosoma cruzi, like other trypanosomatids analyzed so far, can use both glucose and amino acids as carbon and energy source. in these parasites, glycolysis is compartmentalized in glycosomes, authentic but specialized peroxisomes. the major part of this pathway, as well as a two-branched glycolytic auxiliary system, are present in these organelles. the first enzyme of one branch of this auxiliary system is the ppi-dependent pyruvate phosphate dikinase (ppdk) that converts phosphoenolpyruvate ... | 2016 | 27003459 |
| cost-effectiveness of blood donation screening for trypanosoma cruzi in mexico. | an estimated 2 million inhabitants are infected with chagas disease in mexico, with highest prevalence coinciding with highest demographic density in the southern half of the country. after vector-borne transmission, trypanosoma cruzi is principally transmitted to humans via blood transfusion. despite initiation of serological screening of blood donations or donors for t. cruzi since 1990 in most latin american countries, mexico only finally included mandatory serological screening nationwide in ... | 2016 | 27002523 |
| curcumin enhances the anti-trypanosoma cruzi activity of benznidazole-based chemotherapy in acute experimental chagas disease. | although curcumin can increase the effectiveness of drugs against malaria, combination therapies using the molecule have never been investigated in chagas disease (chd). therefore, we evaluated the efficacy of curcumin as a complementary strategy to benznidazole (bz)-based chemotherapy in mice acutely infected with trypanosoma cruzi eighty-four 12-week-old swiss mice were equally randomized into seven groups: uninfected (ni), t. cruzi infected and untreated (inf), infected and treated with 100 m ... | 2016 | 27001816 |
| binding mode and selectivity of steroids towards glucose-6-phosphate dehydrogenase from the pathogen trypanosoma cruzi. | glucose-6-phosphate dehydrogenase (g6pdh) plays a housekeeping role in cell metabolism by generating reducing power (nadph) and fueling the production of nucleotide precursors (ribose-5-phosphate). based on its indispensability for pathogenic parasites from the genus trypanosoma, g6pdh is considered a drug target candidate. several steroid-like scaffolds were previously reported to target the activity of g6pdh. epiandrosterone (ea) is an uncompetitive inhibitor of trypanosomal g6pdh for which it ... | 2016 | 26999093 |
| identification of "preferred" human kinase inhibitors for sleeping sickness lead discovery. are some kinases better than others for inhibitor repurposing? | a kinase-targeting cell-based high-throughput screen (hts) against trypanosoma brucei was recently reported, and this screening set included the published kinase inhibitor set (pkis). from the pkis was identified 53 compounds with pec50 ≥ 6. utilizing the published data available for the pkis, a statistical analysis of these active antiparasitic compounds was performed, allowing identification of a set of human kinases having inhibitors that show a high likelihood for blocking t. brucei cellular ... | 2016 | 26998514 |
| tgf-β receptor type ii costameric localization in cardiomyocytes and host cell tgf-β response is disrupted by trypanosoma cruzi infection. | transforming growth factor beta (tgf-β) cytokine is involved in chagas disease establishment and progression. since trypanosoma cruzi can modulate host cell receptors, we analysed the tgf-β receptor type ii (tβrii) expression and distribution during t. cruzi - cardiomyocyte interaction. tβrii immunofluorescent staining revealed a striated organization in cardiomyocytes, which was co-localized with vinculin costameres and enhanced (38%) after tgf-β treatment. cytochalasin d induced a decrease of ... | 2016 | 26996782 |
| molecular docking and binding mode analysis of plant alkaloids as in vitro and in silico inhibitors of trypanothione reductase from trypanosoma cruzi. | trypanothione reductase (tryr) is a key enzyme in the metabolism of trypanosoma cruzi, the parasite responsible for chagas disease. the available repertoire of tryr inhibitors relies heavily on synthetic substrates of limited structural diversity, and less on plant-derived natural products. in this study, a molecular docking procedure using a lamarckian genetic algorithm was implemented to examine the protein-ligand binding interactions of strong in vitro inhibitors for which no x-ray data is av ... | 2016 | 26996020 |
| differential parasitological, molecular, and serological detection of trypanosoma cruzi i, ii, and iv in blood of experimentally infected mice. | trypanosoma cruzi is the etiological agent of american trypanosomiasis (chagas' disease), which affects 6-7 million people worldwide, mainly in latin america. it presents great genetic and biological variability that plays an important role in the clinical and epidemiological features of the disease. our working hypothesis is that the genetic diversity of t. cruzi has an important impact on detection of the parasite using diagnostic techniques. the present study evaluated the diagnostic performa ... | 2016 | 26995535 |
| risk assessment of soulatrolide and mammea (a/ba+a/bb) coumarins from calophyllum brasiliense by a toxicogenomic and toxicological approach. | calophyllum brasiliense (calophyllaceae) is a tropical rain forest tree distributed in central and south america. it is an important source of tetracyclic dipyrano coumarins (soulatrolide) and mammea type coumarins. soulatrolide is a potent inhibitor of hiv-1 reverse transcriptase and displays activity against mycobacterium tuberculosis. meanwhile, mammea a/ba and a/bb, pure or as a mixture, are highly active against several human leukemia cell lines, trypanosoma cruzi and leishmania amazonensis ... | 2016 | 26995226 |
| kdr mutations in triatoma infestans from the gran chaco are distributed in two differentiated foci: implications for pyrethroid resistance management. | point mutations in the voltage-gated sodium channel, the primary target of pyrethroid insecticides, have been associated with the resistance in triatoma infestans, an important vector of chagas' disease. hence, the sustainability of vector control programs requires the implementation of resistance management strategies. we determined the sensitivity of the molecular assays previously designed for early resistance detection to be used in pooled samples from a wide area of the endemic region, and ... | 2016 | 26992297 |
| mechanisms of vascular dysfunction in acute phase of trypanosoma cruzi infection in mice. | vascular disorders have a direct link to mortality in the acute phase of trypanosoma cruzi infection. however, the underlying mechanisms of vascular dysfunction in this phase are largely unknown. we hypothesize that t. cruzi invades endothelial cells causing dysfunction in contractility and relaxation of the mouse aorta. immunodetection of t. cruzi antigen tcrbp28 was observed in endothelial cells. there was a decreased endothelial nitric oxide synthase (enos)-derived no-dependent vascular relax ... | 2016 | 26988253 |
| identifying gaps in research prioritization: the global burden of neglected tropical diseases as reflected in the cochrane database of systematic reviews. | neglected tropical diseases (ntds) impact disadvantaged populations in resource-scarce settings. availability of synthesized evidence is paramount to end this disparity. the aim of the study was to determine whether ntd systematic reviews or protocols in the cochrane database of systematic reviews (cdsr) reflect disease burden. | 2017 | 26985407 |
| trypanosoma cruzi-infected panstrongylus geniculatus and rhodnius robustus adults invade households in the tropics of cochabamba region of bolivia. | there are hardly any data available on the relationships between the parasite and the vector or regarding potential reservoirs involved in the natural transmission cycle of trypanosoma cruzi in the tropics of cochabamba, bolivia. local families from communities were responsible for the capture of triatomine specimens, following a strategic methodology based on entomological surveillance with community participation developed by the national chagas programme of the ministry of health of bolivia. | 2016 | 26984679 |
| trypanosoma cruzi polyamine transporter: its role on parasite growth and survival under stress conditions. | trypanosoma cruzi is the etiological agent of chagas disease, a major health problem in latin america. polyamines are polycationic compounds that play a critical role as regulators of cell growth and differentiation. in contrast with other protozoa, t. cruzi is auxotrophic for polyamines because of its inability to synthesize putrescine due to the lack of both, arginine and ornithine decarboxylase; therefore, the intracellular availability of polyamines depends exclusively on transport processes ... | 2016 | 26983938 |
| trypanocidal and leishmanicidal activities of flavonoids isolated from stevia satureiifolia var. satureiifolia. | context chagas' disease and leishmaniasis produce significant disability and mortality with great social and economic impact. the genus stevia (asteraceae) is a potential source of antiprotozoal compounds. objective aerial parts of four stevia species were screened on trypanosoma cruzi. stevia satureiifolia (lam.) sch. bip. var. satureiifolia (asteraceae) dichloromethane extract was selected for a bioassay-guided fractionation in order to isolate its active compounds. additionally, the antileish ... | 2016 | 26983579 |
| risk factors associated with seropositivity for leishmania spp. and trypanosoma cruzi in dogs in the state of paraiba, brazil. | the aim of this survey was to determine the seropositivity and risk factors for leishmania spp. and trypanosoma cruzi in dogs in the state of paraíba, northeastern brazil. a total of 1,043 dogs were tested, and the serological diagnoses of chagas disease (cd) and canine visceral leishmaniasis (cvl) was performed by the indirect fluorescent antibody test (ifat). animals that tested seropositive for both diseases (by ifat) were further subjected to elisa. of the 1,043 dogs 81 (7.8%; 95% ci = 6.1-9 ... | 2016 | 26982555 |
| pharmacokinetic and pharmacodynamic responses in adult patients with chagas disease treated with a new formulation of benznidazole. | pharmacological treatment of chagas disease with benznidazole (bnz) is effective in children in all stages, but it is controversial in chronically infected adults. we report the pharmacokinetics and pharmacodynamics in six adult patients with chagas disease treated with the new bnz formulation (abarax®) in doses between 2.5-5.5 mg/kg/day. all but one patient had plasmatic bnz concentrations within the expected range. all patients finalised treatment with nondetectable trypanosoma cruzi quantitat ... | 2016 | 26982179 |
| regulation of rna binding proteins in trypanosomatid protozoan parasites. | posttranscriptional mechanisms have a critical role in the overall outcome of gene expression. these mechanisms are especially relevant in protozoa from the genus trypanosoma, which is composed by death threatening parasites affecting people in sub-saharan africa or in the americas. in these parasites the classic view of regulation of transcription initiation to modulate the products of a given gene cannot be applied. this is due to the presence of transcription start sites that give rise to lon ... | 2016 | 26981203 |
| a novel vaccine approach for chagas disease using rare adenovirus serotype 48 vectors. | due to the increasing amount of people afflicted worldwide with chagas disease and an increasing prevalence in the united states, there is a greater need to develop a safe and effective vaccine for this neglected disease. adenovirus serotype 5 (ad5) is the most common adenovirus vector used for gene therapy and vaccine approaches, but its efficacy is limited by preexisting vector immunity in humans resulting from natural infections. therefore, we have employed rare serotype adenovirus 48 (ad48) ... | 2016 | 26978385 |
| chronic chagas disease diagnosis: a comparative performance of commercial enzyme immunoassay tests. | there is a significant heterogeneity in reported performance of serological assays for chagas disease diagnosis. the conventional serology testing in laboratory diagnosis and in blood banks is unsatisfactory because of a high number of inconclusive and misclassified results. we aimed to assess the quality of four commercially available enzyme-linked immunosorbent assay tests for their ability to detect trypanosoma cruzi antibodies in 685 sera samples. cross-reactivity was assessed by using 748 s ... | 2016 | 26976886 |
| altered cardiomyocyte function and trypanosoma cruzi persistence in chagas disease. | chagas disease, caused by the triatominae trypanosoma cruzi, is one of the leading causes of heart malfunctioning in latin america. the cardiac phenotype is observed in 20-30% of infected people 10-40 years after their primary infection. the cardiac complications during chagas disease range from cardiac arrhythmias to heart failure, with important involvement of the right ventricle. interestingly, no studies have evaluated the electrical properties of right ventricle myocytes during chagas disea ... | 2016 | 26976879 |
| knowledge and experiences of chagas disease in bolivian women living in spain: a qualitative study. | in europe, spain has the highest number of people with chagas disease (cd). bolivian migrants account for 81% of the reported cases. one of the priorities in controlling the disease is prevention of mother-to-child transmission. despite under-diagnosis in spain being estimated at 90%, there are currently few studies that explore the social and cultural dimensions of this disease. | 2016 | 26976265 |
| resveratrol inhibits trypanosoma cruzi arginine kinase and exerts a trypanocidal activity. | arginine kinase catalyzes the reversible transphosphorylation between adp and phosphoarginine which plays a critical role in the maintenance of cellular energy homeostasis. arginine kinase from the protozoan parasite trypanosoma cruzi, the etiologic agent of chagas disease, meets the requirements to be considered as a potential therapeutic target for rational drug design including being absent in its mammalian hosts. in this study a group of polyphenolic compounds was evaluated as potential inhi ... | 2016 | 26976067 |
| trypanosoma cruzi differentiates and multiplies within chimeric parasitophorous vacuoles in macrophages coinfected with leishmania amazonensis. | the trypanosomatids leishmania amazonensis and trypanosoma cruzi are excellent models for the study of the cell biology of intracellular protozoan infections. after their uptake by mammalian cells, the parasitic protozoan flagellates l. amazonensis and t. cruzi lodge within acidified parasitophorous vacuoles (pvs). however, whereas l. amazonensis develops in spacious, phagolysosome-like pvs that may enclose numerous parasites, t. cruzi is transiently hosted within smaller vacuoles from which it ... | 2016 | 26975994 |
| evaluation of parasiticide treatment with benznidazol in the electrocardiographic, clinical, and serological evolution of chagas disease. | chagas disease is one of the most important endemic parasitic diseases in latin america. in its chronic phase, progression to cardiomyopathy has high morbidity and mortality. the persistence of a normal electrocardiogram (ecg) provides a similar prognosis to that of a non-diseased population. benznidazole (bnz) is the only drug with trypanocidal action available in brazil. | 2016 | 26974551 |
| promiscuous recognition of a trypanosoma cruzi cd8+ t cell epitope among hla-a2, hla-a24 and hla-a1 supertypes in chagasic patients. | tctle is a nonamer peptide from trypanosoma cruzi kmp-11 protein that is conserved among different parasite strains and that is presented by different hla-a molecules from the a2 supertype. because peptides presented by several major histocompatibility complex (mhc) supertypes are potential targets for immunotherapy, the aim of this study was to determine whether mhc molecules other than the a2 supertype present the tctle peptide. | 2016 | 26974162 |
| the role of the c-terminal region on the oligomeric state and enzymatic activity of trypanosoma cruzi hypoxanthine phosphoribosyl transferase. | hypoxanthine phosphoribosyl transferase from trypanosoma cruzi (tchprt) is a critical enzyme for the survival of the parasite. this work demonstrates that the full-length form in solution adopts a stable and enzymatically active tetrameric form, exhibiting large inter-subunit surfaces. although this protein irreversibly aggregates during unfolding, oligomerization is reversible and can be modulated by low concentrations of urea. when the c-terminal region, which is predicted as a disordered stre ... | 2016 | 26969784 |
| cardiac sympathetic denervation for intractable ventricular arrhythmias in chagas disease. | autonomic modulation is a valuable therapeutic option for the management of ventricular arrhythmias. bilateral cardiac sympathetic denervation (bcsd) has shown promising results in the acute, intermediate, and long-term management of polymorphic and monomorphic ventricular tachycardia (vt) in patients with structural heart disease. cardiomyopathy (cm) due to chagas disease (cd), and associated vt, is thought to be in part due to autonomic neuronal destruction and dysfunction. | 2016 | 26969783 |
| novel drug discovery for chagas disease. | chagas disease is a chronic infection associated with long-term morbidity. increased funding and advocacy for drug discovery for neglected diseases have prompted the introduction of several important technological advances, and chagas disease is among the neglected conditions that has mostly benefited from technological developments. a number of screening campaigns, and the development of new and improved in vitro and in vivo assays, has led to advances in the field of drug discovery. | 2016 | 26967915 |
| compound profiling and 3d-qsar studies of hydrazone derivatives with activity against intracellular trypanosoma cruzi. | chagas disease is a tropical disease caused by the parasite trypanosoma cruzi, which is endemic in central and south america. few treatments are available with effectiveness limited to the early (acute) stage of disease, significant toxicity and widespread drug resistance. in this work we report the outcome of a hts-ready assay chemical library screen to identify novel, nontoxic, small-molecule inhibitors of t. cruzi. we have selected 50 compounds that possess hydrazone as a common group. the co ... | 2016 | 26964673 |
| the driving of immune response by th1 adjuvants in immunization of mice with trypanosoma cruzi marinkellei elicits a controversial infection control. | in previous studies, we have demonstrated that inoculation with a trypanosoma cruzi marinkellei (avirulent rm1 strain) was able to reduce parasitemia in mice challenged with t. cruzi, although it was not able to prevent histopathological lesions. th1 response stimulation by immunization is necessary for t. cruzi infection control, but the resistance is also dependent on immunoregulatory mechanisms, which can be induced by adjuvants. thus, we evaluated whether inoculation of t. cruzi marinkellei ... | 2016 | 26959861 |
| myocardial infarction-associated transcript, a long noncoding rna, is overexpressed during dilated cardiomyopathy due to chronic chagas disease. | long noncoding rnas (lncrnas) modulate gene expression at the epigenetic, transcriptional, and posttranscriptional levels. dysregulation of the lncrna known as myocardial infarction-associated transcript (miat) has been associated with myocardial infarction. chagas disease causes a severe inflammatory dilated chronic cardiomyopathy (ccc). we investigated the role of miat in ccc. a whole-transcriptome analysis of heart biopsy specimens and formalin-fixed, paraffin-embedded samples revealed that m ... | 2016 | 26951817 |
| risk factors and screening for trypanosoma cruzi infection of dutch blood donors. | blood donors unaware of trypanosoma cruzi infection may donate infectious blood. risk factors and the presence of t. cruzi antibodies in at-risk dutch blood donors were studied to assess whether specific blood safety measures are warranted in the netherlands. | 2016 | 26950434 |
| paleopathology of pre-columbian mummies at the museum of anthropology and ethnology in florence. | we performed a histopathological study on the mummified tissue specimens of seven pre-columbian mummies which arrived in italy in the second half of the 19th century and are housed in the section of anthropology and ethnology of the museum of natural history of the university of florence. the results confirm that the modern techniques of pathological anatomy can be successfidly applied on mummifed tissues, so as to perform important paleopathological diagnoses. among the results obtained from th ... | 2015 | 26946597 |
| investigation of calcium channel blockers as antiprotozoal agents and their interference in the metabolism of leishmania (l.) infantum. | leishmaniasis and chagas disease are neglected parasitic diseases endemic in developing countries; efforts to find new therapies remain a priority. calcium channel blockers (ccbs) are drugs in clinical use for hypertension and other heart pathologies. based on previous reports about the antileishmanial activity of dihydropyridine-ccbs, this work aimed to investigate whether the in vitro anti-leishmania infantum and anti-trypanosoma cruzi activities of this therapeutic class would be shared by ot ... | 2016 | 26941821 |
| the mitogen-activated protein kinase (mapk) pathway: role in immune evasion by trypanosomatids. | leishmania spp. and trypanosoma cruzi are the causative agents of leishmaniasis and chagas disease, respectively, two neglected tropical diseases that affect about 25 million people worldwide. these parasites belong to the family trypanosomatidae, and are both obligate intracellular parasites that manipulate host signaling pathways and the innate immune system to establish infection. mitogen-activated protein kinases (mapks) are serine and threonine protein kinases that are highly conserved in e ... | 2016 | 26941717 |
| comparative proteomic analysis of the saliva of the rhodnius prolixus, triatoma lecticularia and panstrongylus herreri triatomines reveals a high interespecific functional biodiversity. | triatomines are hematophagous arthropods that transmit trypanosoma cruzi and trypanosoma rangeli. feeding behavior and pathogen transmission is known to vary between the different species, and this characteristic is directly or indirectly dependent on the bioactive molecules of the saliva that facilitate the vector-host-parasite interaction. here, we identify, characterize and compare the sialoproteomic (from the greek sialo: saliva) repertoire of important species of the main triatomine genera ... | 2016 | 26940473 |
| towards a toolkit for cross-neglected tropical disease morbidity and disability assessment. | neglected tropical diseases (ntds) are a group of often chronic and disabling infectious conditions, closely related to poverty and inequities. while it is estimated that millions of people are affected, accurate and internationally comparable data about ntd-related morbidity and disability are lacking. therefore we aimed to develop and pilot a toolkit to assess and monitor morbidity and disability across ntds. | 2016 | 26940312 |
| social stigma towards neglected tropical diseases: a systematic review. | people affected by neglected tropical diseases (ntds) are frequently the target of social stigmatization. to date not much attention has been given to stigma in relation to ntds. the objective of this review is to identify the extent of social stigma and the similarities and differences in the causes, manifestations, impact of stigma and interventions used between the ntds. | 2016 | 26940310 |
| mode of action of the sesquiterpene lactones psilostachyin and psilostachyin c on trypanosoma cruzi. | trypanosoma cruzi is the causative agent of chagas' disease, which is a major endemic disease in latin america and is recognized by the who as one of the 17 neglected tropical diseases in the world. psilostachyin and psilostachyin c, two sesquiterpene lactones isolated from ambrosia spp., have been demonstrated to have trypanocidal activity. considering both the potential therapeutic targets present in the parasite, and the several mechanisms of action proposed for sesquiterpene lactones, the ai ... | 2016 | 26939119 |
| control and management of congenital chagas disease in europe and other non-endemic countries: current policies and practices. | identifying pregnant women infected with trypanosoma cruzi is one of the major challenges for preventing and controlling chagas disease (cd) in non-endemic countries. the aim of this paper was to perform a policy evaluation of the current practices of congenital chagas disease (ccd) control in non-endemic countries and to propose specific targets for enhanced interventions to tackle this emerging health problem outside the endemic areas of latin america. | 2016 | 26932338 |
| impact of trypanosoma cruzi on antimicrobial peptide gene expression and activity in the fat body and midgut of rhodnius prolixus. | rhodnius prolixus is a major vector of trypanosoma cruzi, the causative agent of chagas disease in latin america. in natural habitats, these insects are in contact with a variety of bacteria, fungi, virus and parasites that they acquire from both their environments and the blood of their hosts. microorganism ingestion may trigger the synthesis of humoral immune factors, including antimicrobial peptides (amps). the objective of this study was to compare the expression levels of amps (defensins an ... | 2016 | 26931761 |
| modeling chagas disease at population level to explain venezuela's real data. | in this paper we present an age-structured epidemiological model for chagas disease. this model includes the interactions between human and vector populations that transmit chagas disease. | 2015 | 26929912 |
| correction for mesquita et al., genome of rhodnius prolixus, an insect vector of chagas disease, reveals unique adaptations to hematophagy and parasite infection. | 2016 | 26929356 | |
| domestic pig (sus scrofa) as an animal model for experimental trypanosoma cruzi infection. | pigs were infected with a bolivian strain of trypanosoma cruzi (genotype i) and evaluated up to 150 days postinoculation (dpi) to determine the use of pigs as an animal model of chagas disease. parasitemia was observed in the infected pigs during the acute phase (15-40 dpi). anti-t. cruzi immunoglobulin m was detected during 15-75 dpi; high levels of anti-t. cruzi immunoglobulin g were detected in all infected pigs from 75 to 150 dpi. parasitic dna was observed by western blot (58%, 28/48) and p ... | 2016 | 26928841 |
| everybody loves sugar: first report of plant feeding in triatomines. | triatomines, which are the vectors of trypanosoma cruzi, have been considered to be exclusive blood feeders for more than 100 years, since the discovery of chagas disease. | 2016 | 26928036 |
| effects of aspirin-triggered resolvin d1 on peripheral blood mononuclear cells from patients with chagas' heart disease. | chagas disease is caused by trypanosoma cruzi (t. cruzi). in some patients with chagas disease, symptoms progress to chronic chagasic cardiomyopathy. endogenously, inflammation is resolved in the presence of lipid mediators such as aspirin-triggered rvd1 (at-rvd1) which has anti-inflammatory and pro-resolution effects. here, we demonstrated, for the first time, the effects of at-rvd1 on t. cruzi antigen-stimulated peripheral blood mononuclear cells (pbmcs) from patients with chagas heart disease ... | 2016 | 26927755 |
| here, there, and everywhere: the ubiquitous distribution of the immunosignaling molecule lysophosphatidylcholine and its role on chagas disease. | chagas disease is a severe illness, which can lead to death if the patients are not promptly treated. the disease is caused by the protozoan parasite trypanosoma cruzi, which is mostly transmitted by a triatomine insect vector. there are 8-10 million people infected with t. cruzi in the world, but the transmission of such disease by bugs occurs only in the americas, especially latin america. chronically infected patients will develop cardiac diseases (30%) and up digestive, neurological, or mixe ... | 2016 | 26925065 |
| norfloxacin and n-donor mixed-ligand copper(ii) complexes: synthesis, albumin interaction, and anti-trypanosoma cruzi activity. | copper(ii) complexes with the first-generation quinolone antibacterial agent norfloxacin containing a nitrogen donor heterocyclic ligand 2,2'-bipyridine (bipy) or 1,10-phenanthroline (phen) were prepared and characterized by ir, epr spectra, molar conductivity, and elemental analyses. the experimental data suggest that norfloxacin was coordinated to copper(ii) through the carboxylato and ketone oxygen atoms. the interaction of the copper(ii) complexes with bovine serum albumin (bsa) and human se ... | 2016 | 26924953 |
| in vitro screening of 2-(1h-imidazol-1-yl)-1-phenylethanol derivatives as antiprotozoal agents and docking studies on trypanosoma cruzi cyp51. | sterol 14α-demethylase (cyp51) is a key enzyme involved in the survival and virulence of many parasite protozoa, such as trypanosoma and leishmania species, thus representing a valuable drug target for the treatment of kinetoplastid diseases. a set of azole-based compounds selected from an in-house compound library was in vitro screened against different human protozoan parasites. several compounds showed selective activity against trypanosoma cruzi, with compound 7 being the most active (ic50 = ... | 2016 | 26922226 |
| antennal phenotype of mexican haplogroups of the triatoma dimidiata complex, vectors of chagas disease. | triatoma dimidiata (latreille) is a species complex that spans north, central, and south america and which is a key vector of all known discrete typing units (dtu) of trypanosoma cruzi, the etiologic agent of chagas disease. morphological and genetic studies indicate that t. dimidiata is a species complex with three principal haplogroups (hg) in mexico. different markers and traits are still inconclusive regarding if other morphological differentiation may indicate probable behavioral and vector ... | 2016 | 26921798 |
| impact of vectorborne parasitic neglected tropical diseases on child health. | chagas disease, leishmaniasis, onchocerciasis and lymphatic filariasis are all vectorborne neglected tropical diseases (ntds) that are responsible for significant disease burden in impoverished children and adults worldwide. as vectorborne parasitic diseases, they can all be targeted for elimination through vector control strategies. examples of successful vector control programmes for these diseases over the past two decades have included the southern cone initiative against chagas disease, the ... | 2016 | 26921274 |
| chronic trypanosoma cruzi infection potentiates adipose tissue macrophage polarization toward an anti-inflammatory m2 phenotype and contributes to diabetes progression in a diet-induced obesity model. | chronic obesity and chagas disease (caused by the protozoan trypanosoma cruzi) represent serious public health concerns. the interrelation between parasite infection, adipose tissue, immune system and metabolism in an obesogenic context, has not been entirely explored. a novel diet-induced obesity model (dio) was developed in c57bl/6 wild type mice to examine the effect of chronic infection (dio+i) on metabolic parameters and on obesity-related disorders. dyslipidemia, hyperleptinemia, and cardi ... | 2016 | 26921251 |
| changes in proteome profile of peripheral blood mononuclear cells in chronic chagas disease. | trypanosoma cruzi (tc) infection causes chagasic cardiomyopathy; however, why 30-40% of the patients develop clinical disease is not known. to discover the pathomechanisms in disease progression, we obtained the proteome signature of peripheral blood mononuclear cells (pbmcs) of normal healthy controls (n/h, n = 30) and subjects that were seropositive for tc-specific antibodies, but were clinically asymptomatic (c/a, n = 25) or clinically symptomatic (c/s, n = 28) with cardiac involvement and le ... | 2016 | 26919708 |
| use of a chagas urine nanoparticle test (chunap) to correlate with parasitemia levels in t. cruzi/hiv co-infected patients. | early diagnosis of reactivated chagas disease in hiv patients could be lifesaving. in latin america, the diagnosis is made by microscopical detection of the t. cruzi parasite in the blood; a diagnostic test that lacks sensitivity. this study evaluates if levels of t. cruzi antigens in urine, determined by chunap (chagas urine nanoparticle test), are correlated with parasitemia levels in t. cruzi/hiv co-infected patients. | 2016 | 26919324 |
| host and parasite genetics shape a link between trypanosoma cruzi infection dynamics and chronic cardiomyopathy. | host and parasite diversity are suspected to be key factors in chagas disease pathogenesis. experimental investigation of underlying mechanisms is hampered by a lack of tools to detect scarce, pleiotropic infection foci. we developed sensitive imaging models to track trypanosoma cruzi infection dynamics and quantify tissue-specific parasite loads, with minimal sampling bias. we used this technology to investigate cardiomyopathy caused by highly divergent parasite strains in balb/c, c3h/hen and c ... | 2016 | 26918803 |
| evolution of the assassin's arms: insights from a phylogeny of combined transcriptomic and ribosomal dna data (heteroptera: reduvioidea). | assassin bugs (reduvioidea) are one of the most diverse (>7,000 spp.) lineages of predatory animals and have evolved an astounding diversity of raptorial leg modifications for handling prey. the evolution of these modifications is not well understood due to the lack of a robust phylogeny, especially at deeper nodes. we here utilize refined data from transcriptomes (370 loci) to stabilize the backbone phylogeny of reduvioidea, revealing the position of major clades (e.g., the chagas disease vecto ... | 2016 | 26916580 |
| total artificial heart as bridge to heart transplantation in chagas cardiomyopathy: case report. | chagas disease (cd) is becoming an increasingly recognized cause of dilated cardiomyopathy outside of latin america, where it is endemic, due to population shifts and migration. heart transplantation (htx) is a therapeutic option for end-stage cardiomyopathy due to cd, but may be considered a relative contraindication due to potential reactivation of the causative organism with immunosuppression therapy. the total artificial heart (tah) can provide mechanical circulatory support in decompensated ... | 2016 | 26915885 |
| symbiont-mediated rna interference in insects. | rna interference (rnai) methods for insects are often limited by problems with double-stranded (ds) rna delivery, which restricts reverse genetics studies and the development of rnai-based biocides. we therefore delegated to insect symbiotic bacteria the task of: (i) constitutive dsrna synthesis and (ii) trauma-free delivery. rnaseiii-deficient, dsrna-expressing bacterial strains were created from the symbionts of two very diverse pest species: a long-lived blood-sucking bug, rhodnius prolixus, ... | 2016 | 26911963 |
| study of mortality from infectious diseases in brazil from 2005 to 2010: risks involved in handling corpses. | in the wake of disasters, the lack of information on how to handle and dispose of corpses leads the professionals involved in emergency operations to uncertainty about associated risks and safety precautions. the article seeks to establish the risks of the etiologic agents involved in brazilian mortality due to infectious diseases and identify and discuss the main protection measures for professionals involved in handling of corpses in disaster situations. it involved a survey of deaths by infec ... | 2016 | 26910156 |
| mortality from neglected tropical diseases in brazil, 2000-2011. | to describe mortality from neglected tropical diseases (ntds) in brazil, 2000-2011. | 2016 | 26908960 |
| structures of trypanosome vacuolar soluble pyrophosphatases: antiparasitic drug targets. | trypanosomatid parasites are the causative agents of many neglected tropical diseases, including the leishmaniases, chagas disease, and human african trypanosomiasis. they exploit unusual vacuolar soluble pyrophosphatases (vsps), absent in humans, for cell growth and virulence and, as such, are drug targets. here, we report the crystal structures of vsp1s from trypanosoma cruzi and t. brucei, together with that of the t. cruzi protein bound to a bisphosphonate inhibitor. both vsp1s form a hybrid ... | 2016 | 26907161 |
| individual and combined antiparasitic effect of six plant metabolites against leishmania amazonensis and trypanosoma cruzi. | six plant metabolites including isobavachalcone (1), 4-hydroxylonchocarpine (2), and (e)-1-(2,2-dimethyl-2h-chromen-6-yl)-3-(4-hydroxyphenyl)prop-2-en-1-one (3), 6,8-(di-3-methyl-but-2-enyl)eriodictyol (4), damnacanthal (5), and buesgenine (6) were evaluated for their leishmanicidal and trypanocidal activities against intracellular amastigotes of leishmania amazonensis and trypanosoma cruzi. compounds 2-4 and 6 displayed antileishmanial activity while 3 and 5 showed trypanocidal effect. the leis ... | 2016 | 26906638 |
| a new antimicrobial protein from the anterior midgut of triatoma infestans mediates trypanosoma cruzi establishment by controlling the microbiota. | the reduviid triatoma infestans is a vector for the protozoan trypanosoma cruzi, the etiological agent of chagas disease. the parasite must address the defense molecules and microbiota that colonize the anterior midgut of t. infestans. to obtain insight into t. cruzi - microbiota interactions in triatomine insects, we characterized a new antimicrobial product from the anterior midgut of t. infestans (tiap) that may be involved in these relationships. the tiap dna fragment was cloned and expresse ... | 2016 | 26905205 |
| anti-parasitic peptides from arthropods and their application in drug therapy. | africa, asia, and latin america are regions highly affected by endemic diseases, such as leishmaniasis, malaria, and chagas' disease. they are responsible for the death of 1000s of patients every year, as there is not yet a cure for them and the drugs used are inefficient against the pathogenic parasites. during the life cycle of some parasitic protozoa, insects become the most important host and disseminator of the diseases triggered by these microorganisms. as infected insects do not develop n ... | 2016 | 26903970 |
| inhibition of carbonic anhydrase from trypanosoma cruzi for the management of chagas disease: an underexplored therapeutic opportunity. | an α-carbonic anhydrases (cas, ec 4.2.1.1) was recently discovered, cloned and characterized in the genome of the protozoan parasite trypanosoma cruzi, the causative agent of chagas disease, a neglected but widespread tropical disease. inhibition of this α-cas (tcca) with anions, sulfonamides, sulfamates, thiols and hydroxamates has been investigated in detail, with several low nanomolar in vitro inhibitors. although the sulfonamides were the best in vitro inhibitors, they showed no ex vivo anti ... | 2016 | 26898220 |
| the catalonian expert patient programme for chagas disease: an approach to comprehensive care involving affected individuals. | the catalonian expert patient programme on chagas disease is a initiative, which is part of the chronic disease programme. it aims to boost responsibility of patients for their own health and to promote self-care. the programme is based on nine sessions conducted by an expert patient. evaluation was focusing in: habits and lifestyle/self-care, knowledge of disease, perception of health, self-esteem, participant satisfaction, and compliance with medical follow-up visits. eighteen participants ini ... | 2017 | 26895150 |
| discovery of novel polyamine analogs with anti-protozoal activity by computer guided drug repositioning. | chagas disease is a parasitic infection caused by the protozoa trypanosoma cruzi that affects about 6 million people in latin america. despite its sanitary importance, there are currently only two drugs available for treatment: benznidazole and nifurtimox, both exhibiting serious adverse effects and limited efficacy in the chronic stage of the disease. polyamines are ubiquitous to all living organisms where they participate in multiple basic functions such as biosynthesis of nucleic acids and pr ... | 2016 | 26891837 |
| molecular individual-based approach on triatoma brasiliensis: inferences on triatomine foci, trypanosoma cruzi natural infection prevalence, parasite diversity and feeding sources. | we used an individual-based molecular multisource approach to assess the epidemiological importance of triatoma brasiliensis collected in distinct sites and ecotopes in rio grande do norte state, brazil. in the semi-arid zones of brazil, this blood sucking bug is the most important vector of trypanosoma cruzi--the parasite that causes chagas disease. first, cytochrome b (cytb) and microsatellite markers were used for inferences on the genetic structure of five populations (108 bugs). second, we ... | 2016 | 26891047 |
| a therapeutic nanoparticle vaccine against trypanosoma cruzi in a balb/c mouse model of chagas disease. | chagas disease, caused by trypanosoma cruzi, results in an acute febrile illness that progresses to chronic chagasic cardiomyopathy in 30% of patients. current treatments have significant side effects and poor efficacy during the chronic phase; therefore, there is an urgent need for new treatment modalities. a robust th1-mediated immune response correlates with favorable clinical outcomes. a therapeutic vaccine administered to infected individuals could bolster the immune response, thereby slowi ... | 2016 | 26890466 |
| concerted efforts to control or eliminate neglected tropical diseases: how much health will be gained? | the london declaration (2012) was formulated to support and focus the control and elimination of ten neglected tropical diseases (ntds), with targets for 2020 as formulated by the who roadmap. five ntds (lymphatic filariasis, onchocerciasis, schistosomiasis, soil-transmitted helminths and trachoma) are to be controlled by preventive chemotherapy (pct), and four (chagas' disease, human african trypanosomiasis, leprosy and visceral leishmaniasis) by innovative and intensified disease management (i ... | 2016 | 26890362 |
| benznidazole extended-release tablets for improved treatment of chagas disease: preclinical pharmacokinetic study. | benznidazole (bnz) is the first-line drug for the treatment of chagas disease. the drug is available in the form of immediate-release tablets for 100-mg (adult) and 12.5-mg (pediatric) doses. the drug is administered two or three times daily for 60 days. the high frequency of daily administrations and the long period of treatment are factors that significantly contribute to the abandonment of therapy, affecting therapeutic success. accordingly, this study aimed to evaluate the preclinical pharma ... | 2016 | 26883698 |
| an nmr biochemical assay for fragment-based drug discovery: evaluation of an inhibitor activity on spermidine synthase of trypanosoma cruzi. | although nmr in fragment-based drug discovery is utilized almost exclusively to evaluate physical binding between molecules, it should be also a powerful tool for biochemical assay, evaluating inhibitory effect of compounds on enzymatic activity. time-dependent spectral change in real-time monitoring or inhibitor concentration-dependent spectral change after constant-time reaction was processed by factor analysis, by which reaction rate or ic50 value was obtained. applications to spermidine synt ... | 2016 | 26881725 |
| neglected tropical protozoan diseases: drug repositioning as a rational option. | neglected tropical diseases represent a major sanitary problem and a huge economic burden to endemic countries, and are currently expanding to non-endemic countries owing to migration currents. though long abandoned in the past, recent research on novel therapeutics has already started to show results. drug repositioning is one of the prominent, more successful strategies to approach the development of new treatments for these diseases. here we present an overview on the limitations of the curre ... | 2016 | 26881713 |
| low seroprevalence of trypanosoma cruzi infection and chronic chagasic cardiomyopathy in a region with abundance of triatomine vectors in yucatan peninsula of mexico. | the yucatan peninsula of mexico is endemic with chagas disease. the main vector responsible for trypanosoma cruzi transmission is triatoma dimidiata which is abundant in domestic, peridomestic and sylvan cycles. the abundance of vectors favours t. cruzi transmission and is a high risk for developing chronic chagasic cardiomyopathy (ccc). in the past 10 years, little information was available on parasite seroprevalence and the prevalence of ccc in the yucatan peninsula. in the present work, we st ... | 2015 | 26878624 |
| chemical characterization and trypanocidal, leishmanicidal and cytotoxicity potential of lantana camara l. (verbenaceae) essential oil. | drug resistance in the treatment of neglected parasitic diseases, such as leishmaniasis and trypanosomiasis, has led to the search and development of alternative drugs from plant origins. in this context, the essential oil extracted by hydro-distillation from lantana camara leaves was tested against leishmania braziliensis and trypanosoma cruzi. the results demonstrated that l. camara essential oil inhibited t. cruzi and l. braziliensis with ic50 of 201.94 μg/ml and 72.31 μg/ml, respectively. l. ... | 2016 | 26875978 |
| in vitro and in vivo identification of tetradentated polyamine complexes as highly efficient metallodrugs against trypanosoma cruzi. | in order to identify new compounds to treat chagas disease during the acute phase with higher activity and lower toxicity than the reference drug benznidazole (bz), a series of tetraamine-based compounds was prepared and their trypanocidal effects against trypanosoma cruzi were evaluated by light microscopy through the determination of ic50 values. cytotoxicity was determined by flow cytometry assays against vero cells. in vivo assays were performed in balb/c mice, in which the parasitemia level ... | 2016 | 26874306 |
| computational drug repositioning by target hopping: a use case in chagas disease. | drug repositioning aims to identify novel indications for existing drugs. one approach to repositioning exploits shared binding sites between the drug targets and other proteins. here, we review the principle and algorithms of such target hopping and illustrate them in chagas disease, an in latin america widely spread, but neglected disease. | 2016 | 26873186 |
| inactivation of the cytosolic and mitochondrial serine hydroxymethyl transferase genes in leishmania major. | leishmania has two serine hydroxylmethyl transferase (shmt) genes, one coding for a cytosolic and the other for a mitochondrial enzyme. trypanosoma cruzi has only the gene coding for the cytosolic enzyme and trypanosoma brucei has no shmt. we tested whether these genes were dispensable for growth in leishmania major. by gene inactivation we succeeded in generating three cells lines one without the cytosolic cshmt, one without the mitochondrial mshmt, and finally one l. major line without any shm ... | 2015 | 26868981 |
| amazonian triatomine biodiversity and the transmission of chagas disease in french guiana: in medio stat sanitas. | the effects of biodiversity on the transmission of infectious diseases now stand as a cornerstone of many public health policies. the upper amazonia and guyana shield are hot-spots of biodiversity that offer genuine opportunities to explore the relationship between the risk of transmission of chagas disease and the diversity of its triatomine vectors. over 730 triatomines were light-trapped in four geomorphological landscapes shaping french-guiana, and we determined their taxonomic status and in ... | 2016 | 26867025 |
| hplc-dad phenolic profile, cytotoxic and anti-kinetoplastidae activity of melissa officinalis. | context melissa officinalis subsp. inodora bornm. (lamiaceae) has been used since ancient times in folk medicine against various diseases, but it has not been investigated against protozoa. objective to evaluate the activities of m. officinalis against leishmania braziliensis, leishmania infantum and trypanosoma cruzi as well as its cytotoxicity in fibroblast cell line. materials and methods the fresh leaves were chopped into 1 cm(2) pieces, washed and macerated with 99.9% of ethanol for 72 h at ... | 2016 | 26864563 |
| low-dose benznidazole treatment results in parasite clearance and attenuates heart inflammatory reaction in an experimental model of infection with a highly virulent trypanosoma cruzi strain. | chagas disease, caused by trypanosoma cruzi, is the main cause of dilated cardiomyopathy in the americas. antiparasitic treatment mostly relies on benznidazole (bzl) due to nifurtimox shortage or unavailability. both induce adverse drug effects (ade) of varied severity in many patients, leading to treatment discontinuation or abandonment. since dosage may influence ade, we aimed to assess bzl efficacy in terms of parasiticidal and anti-inflammatory activity, using doses lower than those previous ... | 2016 | 26862474 |