Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| nitrogen fixation and translocation in young sugarcane (saccharum officinarum l.) plants associated with endophytic nitrogen-fixing bacteria. | the tracer (15)n(2) was used to investigate sites of n(2) fixation and the possible translocation of the fixed n. young sugarcane plants (saccharum officinarum l.) from a stem cutting were exposed to (15)n(2)-labeled air in a 500 ml plastic cylinder. plants fed (15)n(2) for 7 days were grown in normal air for a further chase period. after 21 days, about half of the n originating in the stem cutting had been transported to the shoot and roots, suggesting that the cutting played a role in supplyin ... | 2009 | 21566377 |
| impact of organic fertilizers with and without chemical fertilizers on soil chemical properties and the establishment of nitrogen-fixing bacteria in the rhizosphere. | effects of organic fertilizers with and without the application of chemical fertilizers for seven years as part of a wheat-pearl millet cropping sequence on soil chemical properties and the establishment of nitrogen fixing bacteria in the rhizosphere were examined. the application of farmyard manure, poultry manure, and sugarcane filter cake alone or in combination with chemical fertilizers improved the soil organic c, total n, p, and k status. larger populations of azotobacter chroococcum and r ... | 2008 | 21558724 |
| infection, multiplication and evaluation of the nitrogen-fixing ability of herbaspirillum sp. strain b501gfp1 in sugarcane stems inoculated by the vacuum infiltration method. | the present study was conducted with the aim of finding a suitable delivery method for introducing endophytic bacteria into new sugarcane stalks. stem pieces of cultivars nif8 and ni15 were inoculated with herbaspirillum sp. strain b501gfp1 by the vacuum infiltration method. the inoculated bacteria showed higher levels of nitrogenase activity in cultivar ni15 than nif8. the population density immediately after inoculation (10(7) cfu g [fresh weight](-1)) showed that strain b501gfp1 successfully ... | 2008 | 21558698 |
| broad distribution and phylogeny of anaerobic endophytes of cluster xiva clostridia in plant species including crops. | endophytic clostridia present on various plants as obligate anaerobes were surveyed by terminal restriction fragment length polymorphism (trflp) analysis specific to the clostridial 16s rrna gene. endophytic clostridia were detected in 10 plant types: sugarcane, cultivated rice, corn, tobacco, soybean, bermuda grass, tall fescue, and three mangrove species. phylogenetically, cluster xiva clostridia were detected more frequently than cluster i clostridia in aerial parts. isolation of clostridia f ... | 2008 | 21558691 |
| population genetics of eldana saccharina walker (lepidoptera: pyralidae) and the implications for management using biocontrol. | the african sugarcane stalk borer, eldona saccharina walker (lepidoptera: pyralidae), is widely distributed throughout sub-saharan africa and is an important insect pest of maize and sugarcane. the insect shows significant variation in behaviour, host plant and natural enemy guild in different regions. several attempts to redistribute the natural enemies of e. saccharina from west africa to south africa were unsuccessful. the significant behavioural, host plant and natural enemy variations as we ... | 2010 | 21539263 |
| f(2) screening for resistance to pyramided bacillus thuringiensis maize in louisiana and mississippi populations of diatraea saccharalis (lepidoptera: crambidae). | background: in the mid-southern region of the united states, sugarcane borer, diatraea saccharalis (f.), is a major target pest of transgenic maize expressing bacillus thuringiensis (bt) proteins. novel transgenic maize technologies containing two or more pyramided bt genes for controlling lepidopteran pests have recently become commercially available. insect resistance management (irm) is an important issue in the sustainable use of bt crop technologies. the objective of this study was to deter ... | 2011 | 21538799 |
| molecular analysis of the complete genomic sequences of four isolates of gooseberry vein banding associated virus. | the presence of gooseberry vein banding associated virus (gvbav), a badnavirus in the family caulimoviridae, is strongly correlated with gooseberry vein banding disease in ribes spp. in this study, full-length genomic sequences of four gvbav isolates from different hosts and geographic regions were determined to be 7649-7663 nucleotides. these isolates share identities of 96.4-97.3% for the complete genomic sequence, indicating low genetic diversity among them. the gvbav genome contains three op ... | 2011 | 21533750 |
| injection of air into the headspace improves fermentation of phosphoric acid pretreated sugarcane bagasse by escherichia coli mm170. | microaeration (injecting air into the headspace) improved the fermentation of hemicellulose hydrolysates obtained from the phosphoric acid pretreatment of sugarcane bagasse at 170°c for 10min. in addition, with 10% slurries of phosphoric acid pretreated bagasse (180°c, 10min), air injection into the headspace promoted xylose utilization and increased ethanol yields from 0.16 to 0.20g ethanol/g bagasse dry weight using a liquefaction plus simultaneous saccharification and co-fermentation process ... | 2011 | 21531547 |
| prevalence of plasmid mediated pesticide resistant bacterial assemblages in crop fields. | three crop fields namely paddy sugarcane and tomato exposed to bavistin [methyl (1h-benzimidazol-2-yl) carbomate], monocrotophos[dimethyl(e)-1-methyl-2-(methyl-carbamoyl) vinyl phosphate] and kinado plus [(ez)-2-chloro-3-dimethoxyphosphinoyloxy-x1, x1-diethylbut-2-enamide], respectively were chosen for the present investigation to know the bacterial population and degradation of pesticides. the chemical nature of the soil and water samples from the pesticide contaminated fields was analysed alon ... | 2010 | 21506482 |
| production of sophorolipid glycolipid biosurfactants from sugarcane molasses using starmerella bombicola nbrc 10243. | biosurfactants (bs) are produced by a variety of microorganisms from renewable resources, and have unique properties compared to chemical surfactants. in order to attain efficient production of bs from low-cost materials, we focused our attention on the use of sugarcane molasses. fifteen yeast strains that are known as bs producers were examined for bs productivity from a culture medium consisting of only molasses and water. among the strains tested, only starmerella bombicola nbrc 10243 produce ... | 2011 | 21502725 |
| biological control of red rot in sugarcane by native pyoluteorin-producing pseudomonas putida strain nh-50 under field conditions and its potential modes of action. | background: rhizobacteria have a good potential to suppress soilborne diseases, but their efficacy against sugarcane pests is rarely reported. bacterial strains isolated from the rhizosphere of sugarcane were evaluated for their potential to suppress red rot disease on two susceptible varieties, co-1148 and spf-234, under field conditions. the strains were also characterised for the production of secondary metabolites associated with their antagonistic activity. results: one out of four strains, ... | 2011 | 21495154 |
| mutagenic activity of airborne particulate matter (pm(10)) in a sugarcane farming area (araraquara city, southeast brazil). | brazil contains 25% of the total land planted with sugarcane in the world and is thus one of the major producers. the annual burning of sugarcane fields prior to harvesting emits huge amounts of pyrogenic particles. biomass burning is an important primary and secondary source of aerosol particles. the presence of carbonaceous particles in the inhalable size range makes it important to study this fraction in view of the possible effects on human health and the climate. in this study, the mutageni ... | 2011 | 21481367 |
| biodegradation of basic violet 3 by candida krusei isolated from textile wastewater. | basic violet 3 (bv) belongs to the most important group of synthetic colorants and is used extensively in textile industries. it is considered as xenobiotic compound which is recalcitrant to biodegradation. as candida krusei could not use bv as sole carbon source, experiments were conducted to study the effect of cosubstrates on decolorization of bv in semi synthetic medium using glucose, sucrose, lactose, maltose, yeast extract, peptone, urea and ammonium sulphate. maximum decolorization (74%) ... | 2011 | 21479732 |
| peroxisomal polyhydroxyalkanoate biosynthesis is a promising strategy for bioplastic production in high biomass crops. | polyhydroxyalkanoates (phas) are bacterial carbon storage polymers with diverse plastic-like properties. pha biosynthesis in transgenic plants is being developed as a way to reduce the cost and increase the sustainability of industrial pha production. the homopolymer polyhydroxybutyrate (phb) is the simplest form of these biodegradable polyesters. plant peroxisomes contain the substrate molecules and necessary reducing power for phb biosynthesis, but peroxisomal phb production has not been explo ... | 2011 | 21447054 |
| in vivo genotoxicity evaluation of atrazine and atrazine-based herbicide on fish carassius auratus using the micronucleus test and the comet assay. | atrazine is a selective triazine herbicide used to control broadleaf and grassy weeds mainly in corn, sorghum, sugarcane, pineapple, and other crops, and in conifer reforestation planting fields. it has been showed that atrazine is one of the most frequently detected pesticides in agricultural streams and rivers, over the past two decades. although the toxic properties of atrazine are well known, the data on the genotoxic effects of atrazine on aquatic organisms are rather scarce. thus, in the p ... | 2011 | 21443921 |
| conservation and divergence of plant lhp1 protein sequences and expression patterns in angiosperms and gymnosperms. | floral transition is a critical and strictly regulated developmental process in plants. mutations in arabidopsis like heterochromatin protein 1 (atlhp1)/terminal flower 2 (tfl2) result in early and terminal flowers. little is known about the gene expression, function and evolution of plant lhp1 homologs, except for arabidopsis lhp1. in this study, the conservation and divergence of plant lhp1 protein sequences was analyzed by sequence alignments and phylogeny. lhp1 expression patterns were compa ... | 2011 | 21416255 |
| global climate niche estimates for bioenergy crops and invasive species of agronomic origin: potential problems and opportunities. | the global push towards a more biomass-based energy sector is ramping up efforts to adopt regionally appropriate high-yielding crops. as potential bioenergy crops are being moved around the world an assessment of the climatic suitability would be a prudent first step in identifying suitable areas of productivity and risk. additionally, this assessment also provides a necessary step in evaluating the invasive potential of bioenergy crops, which present a possible negative externality to the bioec ... | 2011 | 21408056 |
| enhanced ethanol production from sugarcane juice by galactose adaptation of a newly isolated thermotolerant strain of pichia kudriavzevii. | the thermotolerant yeast strain isolated from sugarcane juice through enrichment technique was identified as a strain of pichiakudriavzevii (issatchenkiaorientalis) through molecular characterization. the p. kudriavzevii cells adapted to galactose medium produced about 30% more ethanol from sugarcane juice than the non-adapted cells. the recycled cells could be used for four successive cycles without a significant drop in ethanol production. fermentation in a laboratory fermenter with galactose ... | 2011 | 21398115 |
| a rare autosomal recessive condition, congenital erythropoietic porphyria, found in the canefield rat rattus sordidus gould 1858. | congenital erythropoietic porphyria (cep) is a rare autosomal recessive condition that has been reported in humans and in some animals, in which uroporphyrin 1 is deposited in the bones, teeth and urine, resulting in pink coloration and fluorescence of the tissues and urine under long-wave ultraviolet (uv) light. we observed red teeth in nine of 450 canefield rats (rattus sordidus) captured in a small, isolated patch of sugarcane in tully, north queensland, australia. the skeletons of these anim ... | 2008 | 21396071 |
| improvement of ethanol production using saccharomyces cerevisiae by enhancement of biomass and nutrient supplementation. | optimization of ethanol production through addition of substratum and protein-lipid additives was studied. oilseed meal extract was used as protein lipid supplement, while rice husk was used as substratum. the effect of oil seed meal extract and rice husk was observed at varying concentration of medium sugar from 8% to 20%. of the three oil seed meal extracts used, viz. groundnut, safflower, and sunflower, safflower was found to be most efficient. the use of oilseed meal extract at 4% was found ... | 2011 | 21373793 |
| duplex-immunocapture-rt-pcr for detection and discrimination of two distinct potyviruses naturally infecting sugarcane (saccharum spp. hybrid). | a sensitive duplex-immunocapture-rt-pcr (d-ic-rt-pcr) technique was developed for detection and discrimination of taxonomically distinct sugarcane streak mosaic virus (scsmv) and sugarcane mosaic virus (scmv) that naturally infect sugarcane. d-ic-rt-pcr was performed using polyclonal antisera for capture of virions. oligo 5'-d(t)18(agc)-3' as a common reverse primer for both viruses and virus specific forward primers, 5'-aagtggttaaacgcctgtgg-3' and 5'-atgtc(ga)aagaa(ga)atgcgcttgc-3' were used fo ... | 2011 | 21365999 |
| anaerobic thermophilic fermentation for carboxylic acid production from in-storage air-lime-treated sugarcane bagasse. | wet storage and in situ lime pretreatment (50 °c, 1-atm air, 56 days, excess lime loading of 0.3 g ca(oh)(2)/g dry biomass) of sugarcane bagasse (4,000 g dry weight) was performed in a bench-scale pile pretreatment system. under thermophilic conditions (55 °c, nh(4)hco(3) buffer, methane inhibitors), air-lime-treated bagasse (80 wt.%) and chicken manure (20 wt.%) were anaerobically co-digested in 1-l rotary fermentors by a mixed culture of marine microorganisms (galveston, tx). during four-stage ... | 2011 | 21365471 |
| d(-)-lactic acid production by leuconostoc mesenteroides b512 using different carbon and nitrogen sources. | sugar concentration from sugarcane juice and yeast autolysate increased lactic acid production more than the other agro-industrial substrates tested. the concentrations of these two components were further optimized using the plackett-burman design and response surface method. a second-order polynomial regression model estimated that a maximal lactic acid production of 66.11 g/l would be obtained when the optimal values of sugar and yeast autolysate were 116.9 and 44.25 g/l, respectively. to val ... | 2011 | 21360091 |
| immunotherapeutic effects of some sugar cane (saccharum officinarum l.) extracts against coccidiosis in industrial broiler chickens. | present paper reports the effects of aqueous and ethanolic extracts of sugar cane (saccharum officinarum l.) juice and bagasse, respectively on protective immune responses in industrial broiler chickens against coccidiosis. immunotherapeutic efficacies of the extracts were measured by evaluating their effect on body weight gain, oocyst shedding, lesion score, anti-coccidial indices, per cent protection and elicited serum antibody responses against coccidiosis. results revealed a significantly lo ... | 2011 | 21354144 |
| effect of reduced sulfur compounds on the fermentation of phosphoric acid pretreated sugarcane bagasse by ethanologenic escherichia coli. | the addition of reduced sulfur compounds (thiosulfate, cysteine, sodium hydrosulfite, and sodium metabisulfite) increased growth and fermentation of dilute acid hydrolysate of sugarcane bagasse by ethanologenic escherichia coli (strains ly180, emfr9, and mm160). with sodium metabisulfite (0.5mm), toxicity was sufficiently reduced that slurries of pretreated biomass (10% dry weight including fiber and solubles) could be fermented by e. coli strain mm160 without solid-liquid separation or cleanup ... | 2011 | 21353535 |
| production and optimization of cellulase enzyme using aspergillus niger usm ai 1 and comparison with trichoderma reesei via solid state fermentation system. | novel design solid state bioreactor, fermsostat, had been evaluated in cellulase production studies using local isolate aspergillus niger usm ai 1 grown on sugarcane bagasse and palm kernel cake at 1 : 1 (w/w) ratio. under optimised ssf conditions of 0.5 kg substrate; 70% (w/w) moisture content; 30°c; aeration at 4 l/h · g fermented substrate for 5 min and mixing at 0.5 rpm for 5 min, about 3.4 u/g of filter paper activity (fpase) was obtained. at the same time, comparative studies of the enzyme ... | 2010 | 21350665 |
| enzymatic hydrolysis of chemithermomechanically pretreated sugarcane bagasse and samples with reduced initial lignin content. | chemithermomechanical (ctm) processing was used to pretreat sugarcane bagasse with the aim of increasing cell wall accessibility to hydrolytic enzymes. yields of the pretreated samples were in the range of 75-94%. disk refining and alkaline-ctm and alkaline/sulfite-ctm pretreatments yielded pretreated materials with 21.7, 17.8, and 15.3% of lignin, respectively. hemicellulose content was also decreased to some extent. fibers of the pretreated materials presented some external fibrillation, fiber ... | 2011 | 21344677 |
| [sugarcane biopolymer membrane: experimental evaluation in the middle ear]. | new developments on biomaterials are important in surgery. the behavior of a new membrane produced from sugarcane will be evaluated in the middle ear of rats. | 2011 | 21340188 |
| use of soybean vinasses as a germinant medium for a geobacillus stearothermophilus atcc 7953 sterilization biological indicator. | a novel low-cost medium was developed from by-products and wastes from the ethanol agro-industry to replace commercial media in the production of a steam sterilization biological indicator (bi). various recovery media were developed using soybean or sugarcane molasses and vinasse to prepare a self-contained bi. media performance was evaluated by viability and heat resistance (d(121 °c) value) according to regulatory standards. a medium produced with a soybean vinasse ratio of 1:70 (1.4%) (w/v) p ... | 2011 | 21336685 |
| phosphorus speciation in sequentially extracted agro-industrial by-products: evidence from x-ray absorption near edge structure spectroscopy. | the phosphorus (p) in agro-industrial by-products--a potential source of freshwater eutrophication but also a valuable fertilizer--needs to be speciated to evaluate its fate in the environment. we investigated to what extent x-ray absorption near edge structure (xanes) spectroscopy at the p k- and l2.3-edges reflected differences in sequentially extracted filter cakes from sugarcane (saccharum officinarum l.) (fic) and niger seed (guizotia abyssinica cass.; nic) processing industry in ethiopia. ... | 2013 | 21284316 |
| production of astaxanthin from cellulosic biomass sugars by mutants of the yeast phaffia rhodozyma. | astaxanthin is a potential high-value coproduct in an ethanol biorefinery. three mutant strains of the astaxanthin-producing yeast phaffia rhodozyma, which were derived from the parent strain atcc 24202 (ucd 67-210) and designated jtm166, jtm185, and ssm19, were tested for their capability of utilizing the major sugars that can be generated from cellulosic biomass, including glucose, xylose, and arabinose, for astaxanthin production. while all three strains were capable of metabolizing these sug ... | 2011 | 21274657 |
| digestive physiology and characterization of digestive cathepsin l-like proteinase from the sugarcane weevil sphenophorus levis. | sugarcane is an important crop that has recently become subject to attacks from the weevil sphenophorus levis, which is not efficiently controlled with chemical insecticides. this demands the development of new control devices for which digestive physiology data are needed. in the present study, ion-exchange chromatography of s. levis whole midgut homogenates, together with enzyme assays with natural and synthetic substrates and specific inhibitors, demonstrated that a cysteine proteinase is a m ... | 2011 | 21256130 |
| ammonium carboxylate production from sugarcane trash using long-term air-lime pretreatment followed by mixed-culture fermentation. | sugarcane trash (st) was converted to ammonium carboxylates using a novel bioprocessing strategy known as long-term air-lime pretreatment/mixed-culture fermentation. at mild conditions (50°c, 5 weeks, 1-atm air, and excess lime loading of 0.4 g ca(oh)(2)/(g dry biomass)), air-lime pretreatment of st had moderate delignification (64.4%) with little loss in polysaccharides. without employing detoxification, sterility, expensive nutrients, or costly enzymes, the feedstock (80% treated st/20% chicke ... | 2010 | 21232948 |
| short distance movement of genomic negative strands in a host and nonhost for sugarcane mosaic virus (scmv). | in order to obtain an initial and preliminary understanding of host and nonhost resistance in the initial step of potyvirus replication, both positive and negative sugarcane mosaic virus (scmv) strands where traced in inoculated and systemic leaves in host and nonhost resistant maize and sugarcane for one mexican potyviral isolate (scmv-ver1). intermediary replication forms, such as the negative viral strand, seem to only move a short distance as surveyed by rt-pcr analysis and elisa in differen ... | 2011 | 21232133 |
| biotechnology tools in agriculture: recent patents involving soybean, corn and sugarcane. | the technological opportunities opened up by biotechnology in agriculture are diverse, including plant breeding, the partial or total relief of pesticides chemicals usage, the improvement of soil fertility, the improvement of the quality attributes of various foods. specifically, various tricks of biotechnology can be used for higher seed yield, resistance to diseases and insects, better stems and roots, tolerance to drought and heat, and better agronomic quality. a number of recent works consid ... | 2011 | 21226663 |
| bioaccumulation of the synthetic dye basic violet 3 and heavy metals in single and binary systems by candida tropicalis grown in a sugarcane bagasse extract medium: modelling optimal conditions using response surface methodology (rsm) and inhibition kinetics. | single and binary effects of dye basic violet 3 and heavy metals, 'namely', pb(ii) and cd(ii), were investigated for their role in dye and heavy metal bioaccumulation by candida tropicalis that was grown in a sugarcane bagasse extract medium containing 8 g/l, 16 g/l or 24 g/l of sugar. the optimum ph was found to be 4.0 in the single system and 5.0 in the binary system. a central composite design was successfully used to analyse the experimental results. four numerical correlations that were fit ... | 2010 | 21215516 |
| the genome sequence of e. coli w (atcc 9637): comparative genome analysis and an improved genome-scale reconstruction of e. coli. | escherichia coli is a model prokaryote, an important pathogen, and a key organism for industrial biotechnology. e. coli w (atcc 9637), one of four strains designated as safe for laboratory purposes, has not been sequenced. e. coli w is a fast-growing strain and is the only safe strain that can utilize sucrose as a carbon source. lifecycle analysis has demonstrated that sucrose from sugarcane is a preferred carbon source for industrial bioprocesses. | 2011 | 21208457 |
| c4 plants as biofuel feedstocks: optimising biomass production and feedstock quality from a lignocellulosic perspective. | the main feedstocks for bioethanol are sugarcane (saccharum officinarum) and maize (zea mays), both of which are c(4) grasses, highly efficient at converting solar energy into chemical energy, and both are food crops. as the systems for lignocellulosic bioethanol production become more efficient and cost effective, plant biomass from any source may be used as a feedstock for bioethanol production. thus, a move away from using food plants to make fuel is possible, and sources of biomass such as w ... | 2011 | 21205189 |
| genomic evidence of intraspecific recombination in sugarcane mosaic virus. | the sugarcane mosaic virus (scmv) of the genus potyvirus, which primarily affects maize, sugarcane, sorghum, abaca, and grasses, occurs worldwide and causes significant economic loss. using the full genome sequences of scmv and several recombination detection methods, in this study we report that recombination is the major driving force in the evolution and emergence of several new variants of scmv. we reported eight highly significant (p < 0.001) recombination break points, majority of which ar ... | 2010 | 21193953 |
| genetic and phenotypic diversity of plant growth promoting rhizobacteria isolated from sugarcane plants growing in pakistan. | bacteria were isolated from roots of sugarcane varieties grown in the fields of punjab. they were identified by using api20e/ne bacterial identification kits and from sequences of 16s rrna and amplicons of the cpn60 gene. the majority of bacteria were found to belong to the genera of enterobacter, pseudomonas, and klebsiella, but members of genera azospirillum, rhizobium, rahnella, delftia, caulobacter, pannonibacter, xanthomonas, and stenotrophomonas were also found. the community, however, was ... | 2010 | 21193815 |
| identification of new candidate pathogenicity factors in the xylem-invading pathogen xanthomonas albilineans by transposon mutagenesis. | xanthomonas albilineans is a xylem-invading pathogen that produces the toxin albicidin that blocks chloroplast differentiation, resulting in disease symptoms of sugarcane leaf scald. in contrast to other xanthomonads, x. albilineans does not possess a hypersensitive response and pathogenicity type iii secretion system and does not produce xanthan gum. albicidin is the only previously known pathogenicity factor in x. albilineans, yet albicidin-deficient mutant strains are still able to efficientl ... | 2011 | 21190440 |
| quantitative proteomic analysis of the interaction between the endophytic plant-growth-promoting bacterium gluconacetobacter diazotrophicus and sugarcane. | gluconacetobacter diazotrophicus is a plant-growth-promoting bacterium that colonizes sugarcane. in order to investigate molecular aspects of the g. diazotrophicus-sugarcane interaction, we performed a quantitative mass spectrometry-based proteomic analysis by (15)n metabolic labeling of bacteria, root samples, and co-cultures. overall, more than 400 proteins were analyzed and 78 were differentially expressed between the plant-bacterium interaction model and control cultures. a comparative analy ... | 2011 | 21190439 |
| bioconversion of saccharum spontaneum (wild sugarcane) hemicellulosic hydrolysate into ethanol by mono and co-cultures of pichia stipitis ncim3498 and thermotolerant saccharomyces cerevisiae-vs(3). | the lignocellulosic biomass is a low-cost renewable resource for eco-benign liquid fuel 'ethanol'. to resolve the hydrolysis of mixed sugars in lignocellulosic substrate saccharum spontaneum, the microbial co-cultures of pichia stipitis ncim 3498 and thermotolerant saccharomyces cerevisiae-vs(3) were analyzed for efficient bioconversion of mixed sugars into ethanol. among the hydrolysis conditions, the acid hydrolysis released maximum sugars along with furans, phenolics and acetic acid. the acid ... | 2010 | 21185411 |
| weedy lignocellulosic feedstock and microbial metabolic engineering: advancing the generation of 'biofuel'. | lignocellulosic materials are the most abundant renewable organic resources (~200 billion tons annually) on earth that are readily available for conversion to ethanol and other value-added products, but they have not yet been tapped for the commercial production of fuel ethanol. the lignocellulosic substrates include woody substrates such as hardwood (birch and aspen, etc.) and softwood (spruce and pine, etc.), agro residues (wheat straw, sugarcane bagasse, corn stover, etc.), dedicated energy c ... | 2010 | 21181146 |
| a specific, robust, and automated method for routine at-line monitoring of the concentration of cellulases in genetically modified sugarcane plants. | bagasse is one of the waste crop materials highlighted as commercially viable for cellulosic bio-ethanol production via enzymatic conversion to release fermentable sugars. genetically modified sugarcane expressing cellobiohydrolases (cbh), endoglucanase (eg), and β-glucosidases (bg) provide a more cost-effective route to cellulose breakdown compared to culturing these enzymes in microbial tanks. hence, process monitoring of the concentration profile of these key cellulases in incoming batches of ... | 2010 | 21136205 |
| simplified process for ethanol production from sugarcane bagasse using hydrolysate-resistant escherichia coli strain mm160. | hexose and pentose sugars from phosphoric acid pretreated sugarcane bagasse were co-fermented to ethanol in a single vessel (sscf), eliminating process steps for solid-liquid separation and sugar cleanup. an initial liquefaction step (l) with cellulase was included to improve mixing and saccharification (l+sscf), analogous to a corn ethanol process. fermentation was enabled by the development of a hydrolysate-resistant mutant of escherichia coli ly180, designated mm160. strain mm160 was more res ... | 2010 | 21111615 |
| the effect of biomass moisture content on bioethanol yields from steam pretreated switchgrass and sugarcane bagasse. | this study aimed to determine the effect of moisture content of three different feedstocks on overall ethanol yield. switchgrass and sugarcane bagasse from two sources were either soaked in water (∼80% moisture) or left dry (∼12% moisture), and half each of these were impregnated with 3%w/w so(2) and all were steam pretreated. the twelve resulting substrates were compared based on overall sugar recovery after pretreatment, cellulose conversion following enzymatic hydrolysis, and ethanol yield fo ... | 2010 | 21109425 |
| developmental and photosynthetic regulation of δ-endotoxin reveals that engineered sugarcane conferring resistance to 'dead heart' contains no toxins in cane juice. | the phosphoenolpyruvate (pep) carboxylase is regulated at the levels of transcription and post-translation in c4 plants in light and abundantly accumulates in leaf mesophyll cells. we report here developmental and photosynthetic regulation of stably accumulated bacillus thuringiensis δ-endotoxin under the control of pep-c promoter in transgenic sugarcane. in young leaves of plants, the transprotein is accumulated to 39% of the levels in mature leaves (135 ng mg(-1)), and is induced with the cell ... | 2010 | 21104030 |
| gluconacetobacter diazotrophicus levansucrase is involved in tolerance to nacl, sucrose and desiccation, and in biofilm formation. | gluconacetobacter diazotrophicus is a nitrogen-fixing bacterium and endophyte of sugarcane, which expresses levansucrase, a fructosyltransferase exoenzyme with sucrose hydrolytic and levan biosynthetic activities. as a result of their physical properties, the levan can provide protection against stress caused by abiotic or biotic factors and participate in the formation of biofilms. in this study, we investigated the construction and function of a levansucrase-defective mutant of g. diazotrophic ... | 2010 | 21103984 |
| development of a yeast strain for xylitol production without hydrolysate detoxification as part of the integration of co-product generation within the lignocellulosic ethanol process. | the present study verified an applicable technology of xylitol bioconversion as part of the integration of co-product generation within second-generation bioethanol processes. a newly isolated yeast strain, candida tropicalis jh030, was shown to have a capacity for xylitol production from hemicellulosic hydrolysate without detoxification. the yeast gives a promising xylitol yield of 0.71 g(p) g(s)(-1) from non-detoxified rice straw hydrolysate that had been prepared by the dilute acid pretreatme ... | 2010 | 21095119 |
| identification and expression analysis of micrornas and targets in the biofuel crop sugarcane. | micrornas (mirnas) are small regulatory rnas, some of which are conserved in diverse plant genomes. therefore, computational identification and further experimental validation of mirnas from non-model organisms is both feasible and instrumental for addressing mirna-based gene regulation and evolution. sugarcane (saccharum spp.) is an important biofuel crop with publicly available expressed sequence tag and genomic survey sequence databases, but little is known about mirnas and their targets in t ... | 2010 | 21092324 |
| bioethanol production from dedicated energy crops and residues in arkansas, usa. | globally, one of the major technologic goals is to achieve cost-effective lignocellulosic ethanol production from biomass feedstocks. lignocellulosic biomass of four dedicated energy crops [giant reed (arundo donax l.), elephantgrass (pennisetum purpureum (schumach), miscanthus × giganteus (illinois clone), and (clone q42641) {hybrid of miscanthus sinensis anderss. and miscanthus sacchariflorus (maxim)}, hack. called giant miscanthus, and sugarcane clone us 84-1028 (saccharum l. spp. hybrid)] an ... | 2011 | 21086455 |
| steam pretreatment and fermentation of the straw material "paja brava" using simultaneous saccharification and co-fermentation. | pretreatment, enzymatic hydrolysis and simultaneous saccharification and fermentation (ssf) of the south american straw material paja brava were investigated. suitable process conditions for an so₂-catalyzed steam pretreatment of the material were determined and assessed by enzymatic digestibility of obtained fiber slurries for 72 h at a water insoluble solids (wis) content of 2%. the best pretreatment conditions obtained (200 °c, 5 min holding time and 2.5% so₂) gave an overall glucose yield fo ... | 2010 | 21081285 |
| production of rhamnolipids in solid-state cultivation using a mixture of sugarcane bagasse and corn bran supplemented with glycerol and soybean oil. | rhamnolipid biosurfactants are attracting attention due to their low toxicity, high biodegradability, and good ecological acceptability. however, production in submerged culture is made difficult by severe foaming problems. solid-state cultivation (ssc) is a promising alternative production method. in the current work, we report the optimization of rhamnolipid production by pseudomonas aeruginosa ufpeda 614 on a solid substrate containing sugarcane bagasse and corn bran. the best rhamnolipid pro ... | 2010 | 21080163 |
| microbial production of xylitol from d-xylose and sugarcane bagasse hemicellulose using newly isolated thermotolerant yeast debaryomyces hansenii. | a thermotolerant yeast capable of fermenting xylose to xylitol at 40°c was isolated and identified as a strain of debaryomyces hansenii by its sequencing. this paper reports the production of xylitol from d-xylose and sugarcane bagasse hemicellulose by free and ca-alginate immobilized cells of d. hansenii. the efficiency of free and immobilized cells were compared for xylitol production from d-xylose and hemicellulose in batch culture at 40°c. the maximum xylitol produced by free cells was 68.6 ... | 2010 | 21067918 |
| high-yield endoglucanase production by trichoderma harzianum ioc-3844 cultivated in pretreated sugarcane mill byproduct. | the low-cost production of cellulolytic complexes presenting high action at mild conditions and well-balanced cellulase activities is one of the major bottlenecks for the economical viability of the production of cellulosic ethanol. in the present paper, the filamentous fungus trichoderma harzianum ioc-3844 was used for the production of cellulases from a pretreated sugarcane bagasse (namely, cellulignin), by submerged fermentation. this fungal strain produced high contents of endoglucanase acti ... | 2010 | 21048871 |
| production of fibrolytic enzymes by aspergillus japonicus c03 using agro-industrial residues with potential application as additives in animal feed. | solid-state fermentation obtained from different and low-cost carbon sources was evaluated to endocellulases and endoxylanases production by aspergillus japonicus c03. regarding the enzymatic production the highest levels were observed at 30 °c, using soy bran added to crushed corncob or wheat bran added to sugarcane bagasse, humidified with salt solutions, and incubated for 3 days (xylanase) or 6 days (cellulase) with 70% relative humidity. peptone improved the xylanase and cellulase activities ... | 2010 | 21046416 |
| transgenic sugarcane plants expressing high levels of modified cry1ac provide effective control against stem borers in field trials. | to improve transgene expression level, we synthesized a truncated insecticidal gene m-cry1ac by increasing its gc content from 37.4 to 54.8%, based on the codon usage pattern of sugarcane genes, and transferred it into two sugarcane cultivars (roc16 and yt79-177) by microprojectile bombardment. the integration sites and expression pattern of the transgene were determined, respectively, by southern, northern and western blot analyses. the transgenic sugarcane lines produced up to 50 ng cry1ac pro ... | 2010 | 21046242 |
| modeling of phosphorus loads in sugarcane in a low-relief landscape using ontology-based simulation. | water flow and p dynamics in a low-relief landscape manipulated by extensive canal and ditch drainage systems were modeled utilizing an ontology-based simulation model. in the model, soil water flux and processes between three soil inorganic p pools (labile, active, and stable) and organic p are represented as database objects. and user-defined relationships among objects are used to automatically generate computer code (java) for running the simulation of discharge and p loads. our objectives w ... | 2013 | 21043280 |
| [fermentation of two insoluble dietary fibers by colonic microflora in vitro]. | to investigate the rule of insoluble dietary fiber fermentation in intestinal tract. | 2010 | 21033443 |
| c4gem, a genome-scale metabolic model to study c4 plant metabolism. | leaves of c(4) grasses (such as maize [zea mays], sugarcane [saccharum officinarum], and sorghum [sorghum bicolor]) form a classical kranz leaf anatomy. unlike c(3) plants, where photosynthetic co(2) fixation proceeds in the mesophyll (m), the fixation process in c(4) plants is distributed between two cell types, the m cell and the bundle sheath (bs) cell. here, we develop a c(4) genome-scale model (c4gem) for the investigation of flux distribution in m and bs cells during c(4) photosynthesis. c ... | 2010 | 20974891 |
| unprecedented enhancement of transient gene expression from minimal cassettes using a double terminator. | the potential of using vector-free minimal gene cassettes (mgcs) with a double terminator for the enhancement and stabilization of transgene expression was tested in sugarcane biolistic transformation. the mgc system used consisted of the enhanced yellow fluorescent protein (eyfp) reporter gene driven by the maize ubiquitin-1 (ubi) promoter and a single or double terminator from nopaline synthase (tnos) or/and cauliflower mosaic virus 35s (35st). transient eyfp expression from tnos or 35st singl ... | 2010 | 20967448 |
| pectin lyase production by recombinant penicillium griseoroseum strain 105. | recombinant penicillium griseoroseum strain 105 overproduces an extracellular pectin lyase (pl) under the transcriptional control of the strong gpda promoter of aspergillus nidulans. our aim was to evaluate pl production by recombinant p. griseoroseum strain 105 in submerged fermentation system bioreactors biofloiii and biofloiv using 2 or 10 l working volumes under different growth conditions and to analyze the production of cellulase, polygalacturonase, pectin methylesterase, and protease. pl ... | 2010 | 20962906 |
| genomic and evolutionary features of the spi-1 type iii secretion system that is present in xanthomonas albilineans but is not essential for xylem colonization and symptom development of sugarcane leaf scald. | xanthomonas albilineans is the causal agent of sugarcane leaf scald. interestingly, this bacterium, which is not known to be insect or animal associated, possesses a type iii secretion system (t3ss) belonging to the injectisome family salmonella pathogenicity island 1 (spi-1). the t3ss spi-1 of x. albilineans shares only low similarity with other available t3ss spi-1 sequences. screening of a collection of 128 plant-pathogenic bacteria revealed that this t3ss spi-1 is present in only two species ... | 2011 | 20955079 |
| characterization of commercial cellulases and their use in the saccharification of a sugarcane bagasse sample pretreated with dilute sulfuric acid. | this study aimed to correlate the efficiency of enzymatic hydrolysis of the cellulose contained in a sugarcane bagasse sample pretreated with dilute h(2)so(4) with the levels of independent variables such as initial content of solids and loadings of enzymes and surfactant (tween 20), for two cellulolytic commercial preparations. the preparations, designated cellulase i and cellulase ii, were characterized regarding the activities of total cellulases, endoglucanase, cellobiohydrolase, cellobiase, ... | 2011 | 20953894 |
| seroprevalence of toxoplasmosis, leptospirosis and brucellosis in sugarcane field-workers in trinidad and tobago. | sugarcane field-workers, like rice field-workers, livestock farmers and abattoir workers are known to be occupationally exposed to zoonotic agents. the study determined the seroprevalence of immunoglobulins to toxoplasma gondii (igm), leptospira spp (igm) and brucella abortus (igg) in sugarcane field-workers across weighing stations in the island of trinidad. in addition, the association of risk factors to infections by the three zoonoses was investigated. blood samples were collected from conse ... | 2010 | 20931907 |
| succinic acid production from sugarcane bagasse hemicellulose hydrolysate by actinobacillus succinogenes. | succinic acid, a four-carbon diacid, has been the focus of many research projects aimed at developing more economically viable methods of fermenting sugar-containing natural materials. succinic acid fermentation processes also consume co(2), thereby potentially contributing to reductions in co(2) emissions. succinic acid could also become a commodity used as an intermediate in the chemical synthesis and manufacture of synthetic resins and biodegradable polymers. much attention has been given rec ... | 2010 | 20882312 |
| categorizing sugarcane cultivar resistance to the sugarcane aphid and yellow sugarcane aphid (hemiptera: aphididae). | sugarcane (saccharum spp.) in louisiana is colonized by two aphid species, the sugarcane aphid, melanaphis sacchari (zehntner), and the yellow sugarcane aphid, sipha flava (forbes) (hemiptera: aphididae). the main problem associated with m. sacchari is transmission of sugarcane yellow leaf virus, a casual agent of yellow leaf disease whose absence has been added to certification standards for micropropagated sugarcane in louisiana. greenhouse studies were conducted to categorize dominant commerc ... | 2010 | 20857758 |
| expression analysis of sugarcane shaggy-like kinase (susk) gene identified through cdna subtractive hybridization in sugarcane (saccharum officinarum l.). | identification of genes whose expression enables plants to adapt to any kind of stresses is integral to developing stress tolerance in crop plants. in this study, pcr-based cdna suppression subtractive hybridization technique was used to construct sugarcane salt (nacl) stress specific forward and reverse subtracted cdna library. for this, mrnas were pooled from the shoot and root tissues stressed with nacl (200 mm) for various time intervals (0.5 to 18 h). sequencing the clones from the forward ... | 2011 | 20853012 |
| comparative evaluations of cellulosic raw materials for second generation bioethanol production. | to evaluate sugar recoveries and fermentabilities of eight lignocellulosic raw materials following mild acid pretreatment and enzyme hydrolysis using a recombinant strain of zymomonas mobilis. | 2010 | 20849394 |
| structural characteristics and phylogenetic analysis of the mitochondrial genome of the sugarcane borer, diatraea saccharalis (lepidoptera: crambidae). | the sugarcane borer, diatraea saccharalis (f.), is an economically important pest of several major crops in north and south america. there is great concern over potential resistance development to the valuable transgenic bacillus thuringiensis corn. cost-effective monitoring methods, especially regarding molecular markers that could detect early changes in resistance allele frequency in field populations of d. saccharalis, are needed. this article reports the complete mitochondrial genome sequen ... | 2011 | 20849253 |
| biological detoxification of different hemicellulosic hydrolysates using issatchenkia occidentalis cctcc m 206097 yeast. | this work had as its main objective to contribute to the development of a biological detoxification of hemicellulose hydrolysates obtained from different biomass plants using issatchenkia occidentalis cctcc m 206097 yeast. tests with hemicellulosic hydrolysate of sugarcane bagasse in different concentrations were carried out to evaluate the influence of the hydrolysate concentration on the inhibitory compounds removal from the sugarcane bagasse hydrolysate, without reduction of sugar concentrati ... | 2011 | 20844925 |
| sugarcane bagasse enzymatic hydrolysis: rheological data as criteria for impeller selection. | the aim of this work was to select an efficient impeller to be used in a stirred reactor for the enzymatic hydrolysis of sugar cane bagasse. all experiments utilized 100 g (dry weight)/l of steam-pretreated bagasse, which is utilized in brazil for cattle feed. the process was studied with respect to the rheological behavior of the biomass hydrolysate and the enzymatic conversion of the bagasse polysaccharides. these parameters were applied to model the power required for an impeller to operate a ... | 2010 | 20844924 |
| isotachophoresis-based sample preparation of cellulases in sugarcane juice using bovine serum albumin as a model protein. | the foremost requirement of quantification of cellulases expressed in genetically modified sugarcane is an efficient sample clean-up. this work investigates the feasibility of isotachophoresis for this purpose. an electrolyte system comprising a leading electrolyte of 10mm formic acid at ph 9.0 and a terminating electrolyte of 10mm β-alanine was devised and used to perform isotachophoresis of cellulases. the use of a simple front cutting method removed a majority of interfering species in the ju ... | 2010 | 20833399 |
| improving the fermentability of enzymatic hydrolysates of lignocellulose through chemical in-situ detoxification with reducing agents. | inhibitory lignocellulose hydrolysates were treated with the reducing agents dithionite and sulfite to achieve improved fermentability. addition of these reducing agents (in the concentration range 5.0-17.5 mm) to enzymatic hydrolysates of spruce wood or sugarcane bagasse improved processes based on both shf (simultaneous hydrolysis and fermentation) and ssf (simultaneous saccharification and fermentation). the approach was exemplified in ethanolic fermentations with saccharomyces cerevisiae and ... | 2011 | 20822900 |
| bioconversion of novel substrate saccharum spontaneum, a weedy material, into ethanol by pichia stipitis ncim3498. | rising fuel prices and environmental issues have paved the way for the exploration of cellulosic ethanol. however, challenges involving substrate hydrolysis and cost-effectiveness still limit the efficient bioconversion and utilization of cellulosic ethanol. we aimed to evaluate a cheaper and abundantly available wild sugarcane variety, saccharum spontaneum, as the raw substrate for bioconversion of ethanol by pichia stipitis ncim3498. three different strategies for substrate hydrolysis using ac ... | 2011 | 20822899 |
| heat shock modulates phosphorylation status and activity of nucleoside diphosphate kinase in cultured sugarcane cells. | nucleoside diphosphate kinase (ndpk) is involved in the regeneration of nucleoside triphosphates (ntps) through its phosphotransferase activity via an autophosphorylating histidine residue. additionally, autophosphorylation of serine and/or threonine residues is documented for ndpks from various organisms. however, the metabolic significance of serine/threonine phosphorylation has not been well characterized. in this study we report the cloning and characterization of ndpki from cultured sugarca ... | 2010 | 20821213 |
| pretreatment of synthetic dairy wastewater using the sophorolipid-producing yeast candida bombicola. | the presence of high strength fats and oils in dairy industry wastewaters poses serious challenges for biological treatment systems, and, therefore, its pretreatment is necessary in order to remove them. in the present study, synthetic dairy wastewater prepared in the laboratory was pretreated using the sophorolipid-producing yeast candida bombicola in a laboratory-scale bioreactor under batch, fed-batch, and continuous modes of operation. to support the yeast growth, the wastewater was suppleme ... | 2011 | 20821070 |
| a new glycosidic flavonoid from jwarhar mahakashay (antipyretic) ayurvedic preparation. | the aqueous extract of jwarhar mahakashay ayurvedic preparation (from the roots of hemidesmus indicus r. br., rubia cordifolia l., cissampelos pareira l.; fruits of terminalia chebula retz., emblica officinalis gaertn., terminalia bellirica roxb., vitis vinifera l., grewia asiatica l., salvadora persica l. and granules of saccharum officinarum l.) has been used as a traditional antipyretic. experimental studies confirmed its antipyretic-analgesic effect with very low ulcerogenicity and toxicity. ... | 2010 | 20814525 |
| kinetics of lime pretreatment of sugarcane bagasse to enhance enzymatic hydrolysis. | the objective of this work was to determine the optimum conditions of sugarcane bagasse pretreatment with lime to increase the enzymatic hydrolysis of the polysaccharide component and to study the delignification kinetics. the first stage was an evaluation of the influence of temperature, reaction time, and lime concentration in the pretreatment performance measured as glucose release after hydrolysis using a 2(3) central composite design and response surface methodology. the maximum glucose yie ... | 2011 | 20803263 |
| production of cellulases and xylanases under catabolic repression conditions from mutant pr-22 of cellulomonas flavigena. | derepressed mutant pr-22 was obtained by n-methyl-n'-nitro-n-nitrosoguanidine (mnng) mutagenic treatment of cellulomonas flavigena pn-120. this mutant improved its xylanolytic activity from 26.9 to 40 u mg(-1) and cellulolytic activity from 1.9 to 4 u mg(-1); this represented rates almost 2 and 1.5 times higher, respectively, compared to its parent strain growing in sugarcane bagasse. either glucose or cellobiose was added to cultures of c. flavigena pn-120 and mutant pr-22 induced with sugarcan ... | 2011 | 20803244 |
| phylogenetic relationships among saccharum clones in pakistan revealed by rapd markers. | forty sugarcane genotypes (clones), including elite lines, commercial cultivars of saccharum officinarum and s. barberi clones, were fingerprinted with 30 rapd markers, using a pcr-based marker assay. the genetic distance for rapd data was determined according to nei, and relationships between accessions were graphed in a dendrogram. genetic distance values ranging from 16.2 to 86.3% were observed among the 40 sugarcane accessions. the lowest genetic distance was found between genotypes us-406 a ... | 2010 | 20799164 |
| fermentation of cellulosic hydrolysates obtained by enzymatic saccharification of sugarcane bagasse pretreated by hydrothermal processing. | this work aims to evaluate the fermentability of cellulosic hydrolysates obtained by enzymatic saccharification of sugarcane bagasse pretreated by hydrothermal processing using candida guilliermondii fti 20037 yeast. the inoculum was obtained from yeast culture in a medium containing glucose as a carbon source supplemented with rice bran extract, cacl(2).2h(2)o and (nh(4))(2)so(4) in 50 ml erlenmeyer flasks, containing 20 ml of medium, initial 5.5 ph under agitation of an orbital shaker (200 rpm ... | 2010 | 20740373 |
| bacterially expressed dsrna protects maize against scmv infection. | rna interference (rnai) is a sequence-specific, posttranscriptional gene silencing (ptgs) process in plants that is mediated by dsrna homologous to the silenced gene(s). in this study, we report an efficient method to produce dsrna using a bacterial expression system. two fragments of the sugarcane mosaic virus (scmv) cp (coat protein) gene were amplified by rt-pcr, and cloned into the inverted-repeat cloning vector puccrnai. the two recombinant plasmids were transformed individually into e. col ... | 2010 | 20734050 |
| evaluating the use of predatory insects as bioindicators of metals contamination due to sugarcane cultivation in neotropical streams. | streams located in areas of sugarcane cultivation receive high concentrations of metal ions from soils of the adjacent areas causing accumulation of metals in the aquatic sediment. this impact results in environmental problems and leads to bioaccumulation of metal ions in aquatic organisms. in the present study, metal concentrations in different predatory insects were studied in streams near sugarcane cultivation and compared to reference sites. possible utilisation of predatory insects as bioin ... | 2011 | 20711857 |
| nutritional value of sugarcane silage enriched with corn grain, urea, and minerals as feed supplement on growth performance of beef steers grazing stargrass. | the objectives were to evaluate the silage quality of sugarcane silage enriched (as fed) with corn grain ground (10%), urea (1.5%), and mineral premix (0.5%) and its effects as a feed supplement on growth performance of beef steers grazing stargrass. firstly, in micro-silages, whole sugarcane enriched with corn grain, urea, and minerals (wsce) were ensilaged by 0, 20, 40, and 60 days. crude protein (cp) and lactic acid (la) increased linearly (p < 0.05) and true protein decreased linearly (p < 0 ... | 2011 | 20711753 |
| antioxidant pathways are up-regulated during biological nitrogen fixation to prevent ros-induced nitrogenase inhibition in gluconacetobacter diazotrophicus. | gluconacetobacter diazotrophicus, an endophyte isolated from sugarcane, is a strict aerobe that fixates n(2). this process is catalyzed by nitrogenase and requires copious amounts of atp. nitrogenase activity is extremely sensitive to inhibition by oxygen and reactive oxygen species (ros). however, the elevated oxidative metabolic rates required to sustain biological nitrogen fixation (bnf) may favor an increased production of ros. here, we explored this paradox and observed that ros levels are, ... | 2010 | 20697694 |
| genome-wide sequencing data reveals virulence factors implicated in banana xanthomonas wilt. | banana xanthomonas wilt is a newly emerging disease that is currently threatening the livelihoods of millions of farmers in east africa. the causative agent is xanthomonas campestris pathovar musacearum (xcm), but previous work suggests that this pathogen is much more closely related to species xanthomonas vasicola than to x. campestris. we have generated draft genome sequences for a banana-pathogenic strain of xcm isolated in uganda and for a very closely related strain of x. vasicola pathovar ... | 2010 | 20695894 |
| setaria viridis: a model for c4 photosynthesis. | c(4) photosynthesis drives productivity in several major food crops and bioenergy grasses, including maize (zea mays), sugarcane (saccharum officinarum), sorghum (sorghum bicolor), miscanthus x giganteus, and switchgrass (panicum virgatum). gains in productivity associated with c(4) photosynthesis include improved water and nitrogen use efficiencies. thus, engineering c(4) traits into c(3) crops is an attractive target for crop improvement. however, the lack of a small, rapid cycling genetic mod ... | 2010 | 20693355 |
| combined effects of sugarcane bagasse extract and synthetic dyes on the growth and bioaccumulation properties of pichia fermentans mtcc 189. | bioaccumulation of synthetic dyes viz. acid blue 93, direct red 28 and basic violet 3 by growing cells of yeast, pichia fermentans mtcc 189 was investigated in growth media prepared from sugarcane bagasse extract. the maximum dye bioaccumulation was determined at ph 5.0 for all the dyes tested. two kinetic models viz. noncompetitive and uncompetitive models were tested in order to determine the toxic effects of dyes on the specific growth rate of p. fermentans mtcc 189. basic violet 3 was found ... | 2010 | 20692093 |
| production of pectate lyase by penicillium viridicatum rfc3 in solid-state and submerged fermentation. | pectate lyase (pl) was produced by the filamentous fungus penicillium viridicatum rfc3 in solid-state cultures of a mixture of orange bagasse and wheat bran (1 : 1 w/w), or orange bagasse, wheat bran and sugarcane bagasse (1 : 1 : 0.5 w/w), and in a submerged liquid culture with orange bagasse and wheat bran (3%) as the carbon source. pl production was highest (1,500 u ml(-1) or 300 ug(-1) of substrate) in solid-state fermentation (ssf) on wheat bran and orange bagasse at 96 hours. pl production ... | 2010 | 20689719 |
| molecular characterization and rna interference of three midgut aminopeptidase n isozymes from bacillus thuringiensis-susceptible and -resistant strains of sugarcane borer, diatraea saccharalis. | aminopeptidase n (apn) proteins located at the midgut epithelium of some lepidopteran species have been implicated as receptors for insecticidal proteins from bacillus thuringiensis. cdnas of three apn isoforms, dsapn1, dsapn2, and dsapn3, from cry1ab-susceptible (cry1ab-ss) and -resistant (cry1ab-rr) strains of the sugarcane borer, diatraea saccharalis (f.) (lepidoptera: crambidae), were identified and sequenced using reverse transcriptase polymerase chain reaction (rt-pcr) and 5' rapid amplifi ... | 2010 | 20685334 |
| evaluation of hexose and pentose in pre-cultivation of candida guilliermondii on the key enzymes for xylitol production in sugarcane hemicellulosic hydrolysate. | the evaluation of hexose and pentose in pre-cultivation of candida guilliermondii fti 20037 yeast on xylose reductase (xr) and xylitol dehydrogenase (xdh) enzymes activities was performed during fermentation in sugarcane bagasse hemicellulosic hydrolysate. the xylitol production was evaluated by using cells previously growth in 30.0 gl(-1) xylose, 30.0 gl(-1) glucose and in both sugars mixture (30.0 gl(-1) xylose and 2.0 gl(-1) glucose). the vacuum evaporated hydrolysate (80 gl(-1)) was detoxifi ... | 2010 | 20683763 |
| monitoring the colonization of sugarcane and rice plants by the endophytic diazotrophic bacterium gluconacetobacter diazotrophicus marked with gfp and gusa reporter genes. | to evaluate the colonization process of sugarcane plantlets and hydroponically grown rice seedlings by gluconacetobacter diazotrophicus strain pal5 marked with the gusa and gfp reporter genes. | 2010 | 20666985 |
| genetic transformation with untranslatable coat protein gene of sugarcane yellow leaf virus reduces virus titers in sugarcane. | sugarcane yellow leaf syndrome, characterized by a yellowing of the leaf midrib followed by leaf necrosis and growth suppression, is caused by sugarcane yellow leaf virus (scylv). we produced scylv-resistant transgenic sugarcane from a susceptible cultivar (h62-4671) and determined the amount of virus present following inoculation. the transgenic plants were produced through biolistic bombardment of cell cultures with an untranslatable coat protein gene. presence of the transgene in regenerated ... | 2011 | 20661641 |
| application of sequence-independent amplification (sia) for the identification of rna viruses in bioenergy crops. | miscanthus x giganteus, energycane, and panicum virgatum (switchgrass) are three potential biomass crops being evaluated for commercial cellulosic ethanol production. viral diseases are potentially significant threats to these crops. therefore, identification of viruses infecting these bioenergy crops is important for quarantine purposes, virus resistance breeding, and production of virus-free planting materials. the application is described of sequence-independent amplification, for the identif ... | 2010 | 20638415 |
| simple and efficient method for isolating cdna fragments of lea3 genes with potential for wide application in the grasses (poaceae). | cdna fragments of lea3 genes with a high gc content (from 68 to 77%) were found in several poaceae, including sorghum vulgare, saccharum officinarum, oryza officinalis, oryza meyeriana, ampelocalamus calcareus, cynodon dactylon, and zizania latifoli. they were successfully isolated by means of optimal experimental parameters, which included dimethyl sulfoxide as additive and degenerate primers "agetkas" and "agkdktg", and their sequences were analyzed. compared to the method of isolating genes b ... | 2010 | 20623457 |
| identification and characterization of yeasts in sugarcane silages. | to enumerate the micro-organisms and to identify the yeast species present during the ensilage of different sugarcane (saccharum spp.) cultivars. | 2010 | 20618886 |
| gish characterization of erianthus arundinaceus chromosomes in three generations of sugarcane intergeneric hybrids. | within erianthus, a genus close to saccharum, the species e. arundinaceus has the potential to contribute valuable traits to sugarcane, including adaptation to biotic and abiotic stresses and ratooning ability. sugarcane breeders have tried for a long time to use erianthus species in their breeding programs but until recently were constrained by a lack of fertile saccharum x erianthus hybrids. we report here for the first time the chromosome composition of fertile saccharum officinarum x e. arun ... | 2010 | 20616864 |
| valorization of an industrial organosolv-sugarcane bagasse lignin: characterization and use as a matrix in biobased composites reinforced with sisal fibers. | in the present study, the main focus was the characterization and application of the by-product lignin isolated through an industrial organosolv acid hydrolysis process from sugarcane bagasse, aiming at the production of bioethanol. the sugarcane lignin was characterized and used to prepare phenolic-type resins. the analysis confirmed that the industrial sugarcane lignin is of hgs type, with a high proportion of the less substituted aromatic ring p-hydroxyphenyl units, which favors further react ... | 2010 | 20589841 |