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ospex11, a peroxisomal biogenesis factor 11, contributes to salt stress tolerance in oryza sativa.peroxisomes are single membrane-bound organelles, whose basic enzymatic constituents are catalase and h2o2-producing flavin oxidases. previous reports showed that peroxisome is involved in numerous processes including primary and secondary metabolism, plant development and abiotic stress responses. however, knowledge on the function of different peroxisome genes from rice and its regulatory roles in salt and other abiotic stresses is limited. here, a novel prey protein, ospex11 (os03g0302000), w ...201627695459
energetics of acclimation to nacl by submerged, anoxic rice seedlings.our aim was to elucidate how plant tissues under a severe energy crisis cope with imposition of high nacl, which greatly increases ion fluxes and hence energy demands. the energy requirements for ion regulation during combined salinity and anoxia were assessed to gain insights into ion transport processes in the anoxia-tolerant coleoptile of rice.201727694332
the abcs of flower development: mutational analysis of ap1/ful-like genes in rice provides evidence for a homeotic (a)-function in grasses.the well-known abc model describes the combinatorial interaction of homeotic genes in specifying floral organ identities. while the b- and c-functions are highly conserved throughout flowering plants and even in gymnosperms, the a-function, which specifies the identity of perianth organs (sepals and petals in eudicots), remains controversial. one reason for this is that in most plants that have been investigated thus far, with arabidopsis being a remarkable exception, one does not find recessive ...201727689766
studies on the probing stimulants for the white-backed planthopper, sogatella furcifera (homoptera: delphacidae) in rice plant (oryza sativa l.).to elucidate the probing stimulants in rice plants for the white-backed planthopper, sogatella furcifera, bioassay-guided separations were conducted, which led to the isolation of four active compounds. using nmr and lc-ms spectra, their structures were determined as isoorientin 2″-o-(6‴-(e)-feruloyl)glucoside, isoorientin 2″-o-(6‴-(e)-p-coumaroyl)glucoside, tricin 5-o-glucoside, and isoscoparin 2″-o-(6‴-(e)-feruloyl)glucoside.201627685157
an easy method for plant polysome profiling.translation of mrna to protein is a fundamental and highly regulated biological process. polysome profiling is considered as a gold standard for the analysis of translational regulation. the method described here is an easy and economical way for fractionating polysomes from various plant tissues. a sucrose gradient is made without the need for a gradient maker by sequentially freezing each layer. cytosolic extracts are then prepared in a buffer containing cycloheximide and chloramphenicol to im ...201627684295
genome-wide identification and characterization of trna-derived rna fragments in land plants.the manuscript by alves et al. entitled "genome-wide identification and characterization of trna-derived rna fragments in land plants" describes the identification and characterization of trnaderived srna fragments in plants. by combining bioinformatic analysis and genetic and molecular approaches, we show that trf biogenesis does not rely on canonical microrna/sirna processing machinery (i.e., independent of dicer-like proteins). moreover, we provide evidences that the arabidopsis s-like ribonu ...201727681945
fine mapping and candidate gene analysis of qhd5, a novel major qtl with pleiotropism for yield-related traits in rice (oryza sativa l.).a major qtl for heading date, qhd5, was fine-mapped to a 52.59-kb region on the short arm of rice chromosome 5. heading date (hd) is one of the most important traits that enables rice to adapt to seasonal differences and specific growth conditions in diverse growing regions. in this study, a major-effect quantitative trait locus (qtl), qhd5, was resolved as a single medelian factor that causes nil(bg1) and nil(xlj) (two near-isogenic lines (nils) used in our study) to have at a minimum of 10-day ...201727677631
enhanced detoxification and degradation of herbicide atrazine by a group of o-methyltransferases in rice.atrazine (atr) as a toxic herbicide has become one of the seriously environmental contaminants worldwide due to its long-term intensive use in crop production. this study identified novel methyltransferases (mts) involved in detoxification and degradation of atr residues in rice plants. from a subset of mts differentially expressed in atr-exposed rice, forty-four o-methyltransferase genes were investigated. total activities were significantly enhanced by atr in rice tissues. to prove detoxifying ...201627677124
analysis of allelic imbalance in rice hybrids under water stress and association of asymmetrically expressed genes with drought-response qtls.information on the effect of stress on the allele-specific expression (ase) profile of rice hybrids is limited. more so, the association of allelically imbalanced genes to important traits is yet to be understood. here we assessed allelic imbalance (ai) in the heterozygote state of rice under non- and water-stress treatments and determined association of asymmetrically expressed genes with grain yield (gy) under drought stress by in-silico co-localization analysis and selective genotyping. the g ...201627671164
the active space of mexican rice borer pheromone traps.the mexican rice borer, eoreuma loftini (dyar) (lepidoptera: crambidae), is an invasive pest of sugarcane, saccharum spp., rice, oryza sativa l., and other graminaceous crops in the united states. traps baited with the synthetic female sex pheromone of e. loftini are used for monitoring and management of this invasive pest. however, the active space, or radius of attraction, of these traps is not known. two field experiments examined the effect of intertrap distance on trap captures with hexagon ...201627665613
bio-protective microbial agents from rhizosphere eco-systems trigger plant defense responses provide protection against sheath blight disease in rice (oryza sativa l.).sheath blight of rice (oryza sativa l.) caused by rhizoctonia solani is a major disease and attempts are being made to develop microbe based technologies for biocontrol of this pathogen. however, the mechanisms of biocontrol are not fully understood and still require indepth study in the backdrop of emerging concepts in biological systems. the present investigation was aimed at deciphering the mechanisms of biocontrol of sheath blight of rice employing pseudomonas fluorescens and trichoderma har ...201627664749
rare earth elements and (87)sr/(86)sr isotopic characterization of indian basmati rice as potential tool for its geographical authenticity.the increasing demand for premium priced indian basmati rice (oryza sativa) in world commodity market causing fraudulent activities like adulteration, mislabelling. in order to develop authentication method for indian basmati rice, (87)sr/(86)sr ratios and rees composition of basmati rice, soil and water samples were determined and evaluated their ability as geographical tracer in the present study. in addition, the possible source of sr in rice plant has also been examined. basmati rice samples ...201727664633
changes of phenolic-acids and vitamin e profiles on germinated rough rice (oryza sativa l.) treated by high hydrostatic pressure.this study was performed to investigate changes in the phenolic acid and vitamin e profiles of germinated rough rice following high hydrostatic pressure treatment (hpt). rough rice was germinated at 37°c for two days and subjected to 0.1, 10, 30, 50, and 100mpa pressures for 24h. the total phenolic acid content increased from 85.37μg/g at 0.1mpa to 183.52μg/g at 100mpa. the highest gallic acid (4.29μg/g), catechin (9.55μg/g), p-coumaric acid (8.36μg/g), ferulic acid (14.99μg/g), salicylic acid ( ...201727664614
a rice ca2+ binding protein is required for tapetum function and pollen formation.in flowering plants, successful male reproduction requires the sophisticated interaction between somatic anther wall layers and reproductive cells. timely degradation of the innermost tissue of the anther wall layer, the tapetal layer, is critical for pollen development. ca(2+) is a well-known stimulus for plant development, but whether it plays a role in affecting male reproduction remains elusive. here we report a role of defective in exine formation 1 (osdex1) in rice (oryza sativa), a ca(2+) ...201627663411
manganese-induced cadmium stress tolerance in rice seedlings: coordinated action of antioxidant defense, glyoxalase system and nutrient homeostasis.the accumulation of cadmium (cd) alters different physiological and biochemical attributes that affect plant growth and yield. in our study, we investigated the regulatory role of supplemental manganese (mn) on hydroponically grown rice (oryza sativa l. cv. brri dhan29) seedlings under cd-stress conditions. exposure of 14-d-old seedlings to 0.3mm cdcl2 for three days caused growth inhibition, chlorosis, nutrient imbalance, and higher cd accumulation. higher cd uptake caused oxidative stress thro ...201727662772
genomewide association analysis for awn length linked to the seed shattering gene qsh1 in rice.awn is one of the most important domesticated traits in rice (oryza sativa). understanding the genetic basis of awn length is important for grain harvest and production, because long awn length is disadvantageous for both grain harvest and milling. we investigated the awn length of 529 rice cultivars and performed a genomewide association studies (gwas) in the indica and japonica subpopulations, and the whole population. in total, we found 17 loci associated with awn length. of these loci, seven ...201627659335
genomewide analysis of the lateral organ boundaries domain gene family in vitis vinifera.in plants, the transcription factor families have been implicated in many important biological processes. these processes include morphogenesis, signal transduction and environmental stress responses. proteins containing the lateral organ boundaries domain (lbd), which encodes a zinc finger-like domain are only found in plants. this finding indicates that this unique gene family regulates only plant-specific biological processes. lbd genes play crucial roles in the growth and development of plan ...201627659322
egrins (environmental gene regulatory influence networks) in rice that function in the response to water deficit, high temperature, and agricultural environments.environmental gene regulatory influence networks (egrins) coordinate the timing and rate of gene expression in response to environmental signals. egrins encompass many layers of regulation, which culminate in changes in accumulated transcript levels. here, we inferred egrins for the response of five tropical asian rice (oryza sativa) cultivars to high temperatures, water deficit, and agricultural field conditions by systematically integrating time-series transcriptome data, patterns of nucleosom ...201627655842
structure and catalytic mechanism of monodehydroascorbate reductase, mdhar, from oryza sativa l. japonica.ascorbic acid (asa) maintains redox homeostasis by scavenging reactive oxygen species from prokaryotes to eukaryotes, especially plants. the enzyme monodehydroascorbate reductase (mdhar) regenerates asa by catalysing the reduction of monodehydroascorbate, using nadh or nadph as an electron donor. the detailed recycling mechanism of mdhar remains unclear due to lack of structural information. here, we present the crystal structures of mdhar in the presence of cofactors, nicotinamide adenine dinuc ...201627652777
abiotic stress and phytohormones affect enzymic activity of 1-o-(indole-3-acetyl)-β-d-glucose: myo-inositol indoleacetyl transferase from rice (oryza sativa).indole-3-acetic acid (iaa) conjugation is a part of mechanism regulating free auxin concentration. 1-o-(indole-3-acetyl)-β-d-glucose: myo-inositol indoleacetyl transferase (iainos synthase) is an enzyme involved in iaa-ester conjugates biosynthesis. biotic and abiotic stress conditions can modulate auxin conjugates formation in plants. in this study, we investigated effect of plant hormones (iaa, aba, sa and 2,4-d) and abiotic stress (drought and salt stress: 150mm nacl and 300mm nacl) on expres ...201627649324
rice bran protein as a potent source of antimelanogenic peptides with tyrosinase inhibitory activity.rice (oryza sativa) is consumed as a staple food globally, and rice bran, the byproduct, is an unused biomass that is ultimately discarded as waste. thus, in the present study, a technique for producing tyrosinase inhibitory peptides from rice bran protein (rbp) was developed. simultaneous treatment of rbp with chymotrypsin and trypsin produced numerous peptides. subsequently, six tyrosinase inhibitory peptides were isolated from the hydrolysate fractions in a multistep purification protocol, an ...201627648609
transcriptomic comparison between brassica oleracea and rice (oryza sativa) reveals diverse modulations on cell death in response to sclerotinia sclerotiorum.sclerotinia stem rot caused by sclerotinia sclerotiorum is a devastating disease of brassica crops, but not in rice. the leaves of a rice line, a partial resistant (r) and a susceptible (s) brassica oleracea pool that bulked from a resistance-segregating f2 population were employed for transcriptome sequencing before and after inoculation by s. sclerotiorum for 6 and 12 h. distinct transcriptome profiles were revealed between b. oleracea and rice in response to s. sclerotiorum. enrichment analys ...201627647523
rice nac transcription factor onac095 plays opposite roles in drought and cold stress tolerance.the nac (nam, ataf and cuc) transcriptional factors constitute a large family with more than 150 members in rice and some of them have been demonstrated to play crucial roles in plant abiotic stress response. here, we report the characterization of a rice stress-responsive nac gene, onac095, and the exploration of its function in drought and cold stress tolerance.201627646344
differential deposition of h2a.z in combination with histone modifications within related genes in oryza sativa callus and seedling.as a histone variant, h2a.z is highly conserved among species and plays a significant role in diverse cellular processes. here, we generated genome-wide maps of h2a.z in oryza sativa (rice) callus and seedling by combining chromatin immunoprecipitation using h2a.z antibody and high-throughput sequencing. we found a significantly high peak and a small peak of h2a.z distributed at the 5' and 3' ends of highly expressed genes, respectively. h2a.z was also associated with inactive genes in both tiss ...201727643852
overexpression of a glyoxalase gene, osgly i, improves abiotic stress tolerance and grain yield in rice (oryza sativa l.).glyoxalase i (gly i) is a component of the glyoxalase system which is involved in the detoxification of methylglyoxal, a byproduct of glycolysis. in the present study, a gene of rice (oryza sativa l., cv. nipponbare) encoding gly i was cloned and characterized. the quantitative real-time pcr analysis indicated that rice gly i (osgly i) was ubiquitously expressed in root, stem, leaf, leaf sheath and spikelet with varying abundance. osgly i was markedly upregulated in response to nacl, zncl2 and m ...201627639962
ghd2, a constans-like gene, confers drought sensitivity through regulation of senescence in rice.constans (co)-like genes have been intensively investigated for their roles in the regulation of photoperiodic flowering, but very limited information has been reported on their functions in other biological processes. here, we found that a co-like gene, ghd2 (grain number, plant height, and heading date2), which can increase the yield potential under normal growth condition just like its homologue ghd7, is involved in the regulation of leaf senescence and drought resistance. ghd2 is expressed m ...201627638689
a new recessive gene conferring resistance against rice blast.rice blast (causative pathogen magnaporthe oryzae) represents a major biotic constraint over rice production. while numerous genes for resistance have been found in both japonica and indica germplasm, as yet the diversity harbored by aus germplasm has not been widely exploited.201627637926
gad1 encodes a secreted peptide that regulates grain number, grain length, and awn development in rice domestication.cultivated rice (oryza sativa) was domesticated from wild rice (oryza rufipogon), which typically displays fewer grains per panicle and longer grains than cultivated rice. in addition, wild rice has long awns, whereas cultivated rice has short awns or lacks them altogether. these changes represent critical events in rice domestication. here, we identified a major gene, grain number, grain length and awn development1 (gad1), that regulates those critical changes during rice domestication. gad1 is ...201627634315
the germin-like protein osglp2-1 enhances resistance to fungal blast and bacterial blight in rice.this is the first report that glp gene (osglp2-1) is involved in panicle blast and bacterial blight resistance in rice. in addition to its resistance to blast and bacterial blight, osglp2-1 has also been reported to co-localize with a qtls for sheath blight resistance in rice. these suggest that the disease resistance provided by osglp2-1 is quantitative and broad spectrum. its good resistance to these major diseases in rice makes it to be a promising target in rice breeding. rice (oryza sativa) ...201627631432
the effect of green synthesized cuo nanoparticles on callogenesis and regeneration of oryza sativa l.in this study, we have investigated the effect of copper oxide nanoparticles (cuo-nps) on callogenesis and regeneration of oryza sativa l (super basmati, basmati 2000, basmati 370, and basmati 385). in this regard, cuo-nps have been bio-synthesized via azadirachta indica leaf extract. scanning electron microscope (sem) analysis depicts average particle size of 40 ± 5 nm with highly homogenous and spherical morphology. x-ray diffraction (xrd) and fourier transform infrared spectroscopy (ftir) hav ...201627630655
overexpression of oshox32 results in pleiotropic effects on plant type architecture and leaf development in rice.the class iii homeodomain leu zipper (hd-zip iii) gene family plays important roles in plant growth and development. here, we analyze the function of oshox32, an hd-zip iii family member, and show that it exhibits pleiotropic effects on regulating plant type architecture and leaf development in rice.201627624698
phytohormones signaling and crosstalk regulating leaf angle in rice.leaf angle is an important agronomic trait in rice (oryza sativa l.). it affects both the efficiency of sunlight capture and nitrogen reservoirs. the erect leaf phenotype is suited for high-density planting and thus increasing crop yields. many genes regulate leaf angle by affecting leaf structure, such as the lamina joint, mechanical tissues, and the midrib. signaling of brassinosteroids (br), auxin (iaa), and gibberellins (ga) plays important roles in the regulation of lamina joint bending in ...201627623811
building two indica rice reference genomes with pacbio long-read and illumina paired-end sequencing data.over the past 30 years, we have performed many fundamental studies on two oryza sativa subsp. indica varieties, zhenshan 97 (zs97) and minghui 63 (mh63). to improve the resolution of many of these investigations, we generated two reference-quality reference genome assemblies using the most advanced sequencing technologies. using pacbio smrt technology, we produced over 108 (zs97) and 174 (mh63) gb of raw sequence data from 166 (zs97) and 209 (mh63) pools of bac clones, and generated ~97 (zs97) a ...201627622467
the hvnramp5 transporter mediates uptake of cadmium and manganese, but not iron.the natural resistance associated macrophage protein (nramp) represents a transporter family for metal ions in all organisms. here, we functionally characterized a member of nramp family in barley (hordeum vulgare), hvnramp5. this member showed different expression patterns, transport substrate specificity, and cellular localization from its close homolog in rice (oryza sativa), osnramp5, although hvnramp5 was also localized to the plasma membrane. hvnramp5 was mainly expressed in the roots and ...201627621428
two different transcripts of a lammer kinase gene play opposite roles in disease resistance.alternative splicing of genes can increase protein diversity and affect mrna stability. genome-wide transcriptome sequencing has demonstrated that alternative splicing occurs in a large number of intron-containing genes of different species. however, despite the phenomenon having been known for decades, it is largely unknown how the alternatively spliced transcripts function differently. here, we report that two alternatively spliced transcripts of the rice (oryza sativa) lammer kinase gene osdr ...201627621422
constans-like 9 (col9) delays the flowering time in oryza sativa by repressing the ehd1 pathway.flowering or heading is one of most important agronomic traits in rice. it has been characterized that constans (co) and constans-like (col) proteins are critical flowering regulators in response to photoperiodic stress in plants. we have previously identified that the col family member oscol9 can positively enhance the rice blast resistance. in the present study, we aimed to explore the functional role of oscol9 in modulating the photoperiodic flowering. our data showed that overexpression of o ...201627620492
comparative proteomic analysis reveals alterations in development and photosynthesis-related proteins in diploid and triploid rice.polyploidy has pivotal influences on rice (oryza sativa l.) morphology and physiology, and is very important for understanding rice domestication and improving agricultural traits. diploid (dp) and triploid (tp) rice shows differences in morphological parameters, such as plant height, leaf length, leaf width and the physiological index of chlorophyll content. however, the underlying mechanisms determining these morphological differences are remain to be defined. to better understand the proteomi ...201627619227
linking hydrogen-mediated boron toxicity tolerance with improvement of root elongation, water status and reactive oxygen species balance: a case study for rice.boron is essential for plant growth but hazardous when present in excess. as the antioxidant properties of hydrogen gas (h2) were recently described in plants, oxidative stress induced by excess boron was investigated along with other biological responses during rice (oryza sativa) seed germination to study the beneficial role of h2 methods: rice seeds were pretreated with exogenous h2 using physiological, pharmacological and molecular approaches, the production of endogenous h2, growth status, ...201627616208
effects of alkaline and bioorganic amendments on cadmium, lead, zinc, and nutrient accumulation in brown rice and grain yield in acidic paddy fields contaminated with a mixture of heavy metals.paddy soils and rice (oryza sativa l.) contaminated by mixed heavy metals have given rise to great concern. field experiments were conducted over two cultivation seasons to study the effects of steel slag (ss), fly ash (fa), limestone (ls), bioorganic fertilizer (bf), and the combination of ss and bf (ssbf) on rice grain yield, cd, pb, and zn and nutrient accumulation in brown rice, bioavailability of cd, pb, and zn in soil as well as soil properties (ph and catalase), at two acidic paddy fields ...201627614643
genetic diversity and phenotypic variation in an introgression line population derived from an interspecific cross between oryza glaberrima and oryza sativa.the introduction of closely related species genomic fragments is an effective way to enrich genetic diversity and creates new germplasms in crops. here, we studied the genetic diversity of an introgression line (il) population composed of 106 ils derived from an interspecific tetra cross between o. glaberrima and o. sativa (ram3/jin23b//jin23b///yuetaib). the proportion of o. glaberrima genome (pgg) in the ils ranged from 0.3% to 36.7%, with an average value of 12.32% which is close to the theor ...201627603678
comparison of saccharification and fermentation of steam exploded rice straw and rice husk.rice cultivation produces two waste streams, straw and husk, which could be exploited more effectively. chemical pretreatment studies using rice residues have largely focussed on straw exploitation alone, and often at low substrate concentrations. moreover, it is currently not known how rice husk, the more recalcitrant residue, responds to steam explosion without the addition of chemicals.201627602056
p31comet, a member of the synaptonemal complex, participates in meiotic dsb formation in rice.the human mitotic arrest-deficient 2 (mad2) binding protein p31(comet) participates in the spindle checkpoint and coordinates cell cycle events in mitosis although its function in meiosis remains unknown in all organisms. here, we reveal p31(comet) as a synaptonemal complex (sc) protein in rice (oryza sativa l.). in p31(comet), homologous pairing and synapsis are eliminated, leading to the homologous nondisjunction and complete sterility. the failure in loading of histone h2ax phosphorylation (γ ...201627601671
morphogenic regulators baby boom and wuschel improve monocot transformation.while transformation of the major monocot crops is currently possible, the process typically remains confined to one or two genotypes per species, often with poor agronomics, and efficiencies that place these methods beyond the reach of most academic laboratories. here, we report a transformation approach involving overexpression of the maize (zea mays) baby boom (bbm) and maize wuschel2 (wus2) genes, which produced high transformation frequencies in numerous previously non-transformable maize i ...201627600536
dna transposon activity is associated with increased mutation rates in genes of rice and other grasses.dna (class 2) transposons are mobile genetic elements which move within their 'host' genome through excising and re-inserting elsewhere. although the rice genome contains tens of thousands of such elements, their actual role in evolution is still unclear. analysing over 650 transposon polymorphisms in the rice species oryza sativa and oryza glaberrima, we find that dna repair following transposon excisions is associated with an increased number of mutations in the sequences neighbouring the tran ...201627599761
phosphoenolpyruvate carboxylase regulation in c4-pepc-expressing transgenic rice during early responses to drought stress.phosphoenolpyruvate carboxylase (pepc; ec 4.1.1.31) has important functions in c4 photosynthesis and biosynthesis of intermediate metabolites. in this study, the drought resistance of c4-pepc-expressing transgenic rice (oryza sativa, line pc) plants was assessed using simulated drought conditions [i.e. polyethylene glycol (peg)-6000 treatment]. the dry weight of pc plants was higher than that of wild-type (wt) plants following treatment with 15% peg-6000 for 16 days. furthermore, the water use e ...201727592839
phosphorus uptake, partitioning and redistribution during grain filling in rice.in cultivated rice, phosphorus (p) in grains originates from two possible sources, namely exogenous (post-flowering root p uptake from soil) or endogenous (p remobilization from vegetative parts) sources. this study investigates p partitioning and remobilization in rice plants throughout grain filling to resolve contributions of p sources to grain p levels in rice.201627590335
population genomics identifies the origin and signatures of selection of korean weedy rice.weedy rice is the same biological species as cultivated rice (oryza sativa); it is also a noxious weed infesting rice fields worldwide. its formation and population-selective or -adaptive signatures are poorly understood. in this study, we investigated the phylogenetics, population structure and signatures of selection of korean weedy rice by determining the whole genomes of 30 weedy rice, 30 landrace rice and ten wild rice samples. the phylogenetic tree and results of ancestry inference study c ...201727589078
plastidic phosphoglucomutase and adp-glucose pyrophosphorylase mutants impair starch synthesis in rice pollen grains and cause male sterility.to elucidate the starch synthesis pathway and the role of this reserve in rice pollen, we characterized mutations in the plastidic phosphoglucomutase, osppgm, and the plastidic large subunit of adp-glucose (adp-glc) pyrophosphorylase, osagpl4 both genes were up-regulated in maturing pollen, a stage when starch begins to accumulate. progeny analysis of self-pollinated heterozygous lines carrying the osppgm mutant alleles, osppgm-1 and osppgm-2, or the osagpl4 mutant allele, osagpl4-1, as well as ...201627588462
a nucleus-encoded chloroplast protein yl1 is involved in chloroplast development and efficient biogenesis of chloroplast atp synthase in rice.chloroplast atp synthase (cpatpase) is an importance thylakoid membrane-associated photosynthetic complex involved in the light-dependent reactions of photosynthesis. in this study, we isolated and characterized a rice (oryza sativa) mutant yellow leaf 1 (yl1), which exhibits chlorotic leaves throughout developmental stages. the yl1 mutation showed reduced chlorophyll contents, abnormal chloroplast morphology, and decreased photochemical efficiency. moreover, yl1 deficiency disrupts the expressi ...201627585744
association analysis in rice: from application to utilization.association analysis based on linkage disequilibrium (ld) is an efficient way to dissect complex traits and to identify gene functions in rice. although association analysis is an effective way to construct fine maps for quantitative traits, there are a few issues which need to be addressed. in this review, we will first summarize type, structure, and ld level of populations used for association analysis of rice, and then discuss the genotyping methods and statistical approaches used for associa ...201627582745
nutritional value and proteases of lentinus citrinus produced by solid state fermentation of lignocellulosic waste from tropical region.this paper examined the growth and yield performance of lentinus citrinus on cupuaçu exocarp (theobroma grandiflorum) mixed with litter (ce + li) or rice bran (oryza sativa) (ce + rb) in the ratio of 2:1 (800 g:200 g) to investigate the nutritional composition and proteolytic potential of the fruiting body produced. significance values of yield were determined on substrate combinations. in ce + li the biological efficiency of the mushrooms was 93.5% and the content of fat (4.5%), fiber (11.0%), ...201627579012
the rice yabby4 gene regulates plant growth and development through modulating the gibberellin pathway.yabby genes encode seed plant-specific transcription factors that play pivotal roles in diverse aspects of leaf, shoot, and flower development. members of the yabby gene family are primarily expressed in lateral organs in a polar manner and function to specify abaxial cell fate in dicotyledons, but this polar expression is not conserved in monocotyledons. the function of yabby genes is therefore not well understood in monocotyledons. here we show that overexpression of the rice (oryza sativa l.) ...201627578842
young seedling stripe1 encodes a chloroplast nucleoid-associated protein required for chloroplast development in rice seedlings.young seedling stripe1 (yss1) was characterized as an important regulator of plastid-encoded plastid rna polymerase (pep) activity essential for chloroplast development at rice seedling stage. chloroplast development is coordinately regulated by plastid- and nuclear-encoding genes. although a few regulators have been reported to be involved in chloroplast development, new factors remain to be identified, given the complexity of this process. here, we report the characterization of a temperature- ...201727578095
phosphate uptake and allocation - a closer look at arabidopsis thaliana l. and oryza sativa l.this year marks the 20th anniversary of the discovery and characterization of the two arabidopsis pht1 genes encoding the phosphate transporter in arabidopsis thaliana. so far, multiple inorganic phosphate (pi) transporters have been described, and the molecular basis of pi acquisition by plants has been well-characterized. these genes are involved in pi acquisition, allocation, and/or signal transduction. this review summarizes how pi is taken up by the roots and further distributed within two ...201627574525
wsl3, a component of the plastid-encoded plastid rna polymerase, is essential for early chloroplast development in rice.plastid-encoded plastid rna polymerase (pep), a dominant rna polymerase in mature chloroplasts, consists of core subunits and peripheral subunits. despite the importance of the peripheral subunits in control of pep activity it is unclear how they interact with one another to exert physiological effects on chloroplast development and plant growth, especially in rice. here, we report a mutant, designated wsl3 that lacks a peripheral subunit in rice. we isolated the wsl3 gene encoding an essential ...201627573887
involvement of polyamine oxidase-produced hydrogen peroxide during coleorhiza-limited germination of rice seeds.seed germination is a complicated biological process that requires regulated enzymatic and non-enzymatic reactions. the action of polyamine oxidase (pao) produces hydrogen peroxide (h2o2), which promotes dicot seed germination. however, whether and, if so, how paos regulate monocot seed germination via h2o2 production is unclear. herein, we report that the coleorhiza is the main physical barrier to radicle protrusion during germination of rice seed (a monocot seed) and that it does so in a manne ...027570530
mirna: a novel link between rice ragged stunt virus and oryza sativa.rice ragged stunt disease caused leads to severe loss of rice yield. recently, rice ragged stunt virus (rrsv) were found to be increasingly common in rice-growing regions of china and vietnam. rrsv may cause problem by interacting with micrornas (mirnas) of host cells and the mechanism is not clear yet. in this study we identified 11 mirnas in response to rrsv infection and predicted their possible targets to viral rna segments (s1-s10) through the bioinformatics analysis. interestingly, we foun ...201627570315
reconstruction of the astaxanthin biosynthesis pathway in rice endosperm reveals a metabolic bottleneck at the level of endogenous β-carotene hydroxylase activity.astaxanthin is a high-value ketocarotenoid rarely found in plants. it is derived from β-carotene by the 3-hydroxylation and 4-ketolation of both ionone end groups, in reactions catalyzed by β-carotene hydroxylase and β-carotene ketolase, respectively. we investigated the feasibility of introducing an extended carotenoid biosynthesis pathway into rice endosperm to achieve the production of astaxanthin. this allowed us to identify potential metabolic bottlenecks that have thus far prevented the ac ...201727567632
oral immunisation of mice with transgenic rice calli expressing cholera toxin b subunit fused to consensus dengue cediii antigen induces antibodies to all four dengue serotypes.dengue virus (denv) infection is an emerging global health threat. denv consists of four distinct serotypes, necessitating a tetravalent vaccine. in this study, expression of consensus envelope protein domain iii (cediii) fused to cholera toxin b subunit (ctb) in transgenic rice calli was improved using the luminal binding protein bip at the n-terminus and the sekdel signal sequences at the c-terminus, targeting the recombinant protein to endoplasmic reticulum (er). we found that the fusion prot ...201627566485
differential regulation of protochlorophyllide oxidoreductase abundances by virescent 5a (osv5a) and virescent 5b (osv5b) in rice seedlings. 201627565208
damage by tibraca limbativentris stål (pentatomidae) to upland rice cultivated in amazon rainforest region (brazil) at different growth stages.in this study, we evaluated the damage caused in the field by tibraca limbativentris stål adults at different levels of infestation (0, 1, 2, and 4 stink bugs) per three rice plants during three growth stages (v8, v13, and r4 stages) of upland rice cultivated in southwestern of pará state, amazon rainforest region, brazil. heading time (panicle exertion) was affected by t. limbativentris infestations mainly in the vegetative stage and the whiteheads percentage in treatments ranged from 18.2 to 3 ...201727558273
one-step generation of chromosomal rearrangements in rice.the combination of the dna sequence-specific recombination system cre/loxp and the dna transposon system activator (ac)/dissociation (ds) has been used for insertional and deletional mutagenesis, as well as for the generation of artificial ring chromosomes in model plants such as arabidopsis and tobacco. however, it takes a long time to complete this process, even in arabidopsis. to overcome this issue, a new binary vector, pdlhc, has been developed to induce chromosomal rearrangements for a sho ...201627557686
generation and analysis of transposon ac/ds-induced chromosomal rearrangements in rice plants.closely-located transposable elements (tes) have been known to induce chromosomal breakage and rearrangements via alternative transposition. to study genome rearrangements in rice, an ac/ds system has been employed. this system comprises an immobile ac element expressed under the control of camv 35s promoter, and a modified ds element. a starter line carried ac and a single copy of ds at the osrlg5 (oryza sativa receptor-like gene 5). to enhance the transpositional activity, seed-derived calli w ...201627557685
qtl detection and elite alleles mining for stigma traits in oryza sativa by association mapping.stigma traits are very important for hybrid seed production in oryza sativa, which is a self-pollinated crop; however, the genetic mechanism controlling the traits is poorly understood. in this study, we investigated the phenotypic data of 227 accessions across 2 years and assessed their genotypic variation with 249 simple sequence repeat (ssr) markers. by combining phenotypic and genotypic data, a genome-wide association (gwa) map was generated. large phenotypic variations in stigma length (stl ...201627555858
a mutagenic study identifying critical residues for the structure and function of rice manganese transporter osmtp8.1.rice (oryza sativa) mtp8.1 (osmtp8.1) is a tonoplast-localized manganese transporter of the cation diffusion facilitator family. here we present a structure-function analysis of osmtp8.1 based on the site-directed and random mutagenesis and complementation assays in manganese hypersensitive yeast, in combination with three-dimensional (3d) structure modeling based on the crystal structure of the escherichia coli cdf family member, ecyiip. two metal-binding sites are conserved in osmtp8.1 with ec ...201627555514
rolling-circle amplification of centromeric helitrons in plant genomes.the unusual eukaryotic helitron transposons can readily capture host sequences and are, thus, evolutionarily important. they are presumed to amplify by rolling-circle replication (rcr) because some elements encode predicted proteins homologous to rcr prokaryotic transposases. in support of this replication mechanism, it was recently shown that transposition of a bat helitron generates covalently closed circular intermediates. another strong prediction is that rcr should generate tandem helitron ...201627553634
lanthanum regulates the reactive oxygen species in the roots of rice seedlings.in this study, the effects of la(3+) on the reactive oxygen species (ros) and antioxidant metabolism were studied in the roots of rice (oryza sativa l. cv shengdao 16) exposed to increasing concentrations of la(3+) (0.05, 0.1, 0.5, 1.0, and 1.5 mm). the level of hydrogen peroxide, superoxide anion, and malondialdehyde was increased by 0.5, 1.0 and 1.5 mm la(3+), and the activity of catalase and peroxidase was increased by 0.05 and 0.1 mm la(3+). however, la(3+) treatments stimulated superoxide d ...201627546334
abnormal vascular bundles regulates cell proliferation and procambium cell establishment during aerial organ development in rice.to understand the molecular mechanisms of rice aerial organ development, we identified a mutant gene that caused a significant decrease in the width of aerial organs, termed abnormal vascular bundles (avb). histological analysis showed that the slender aerial organs were caused by cell number reduction. in avb, the number of vascular bundles in aerial organs was reduced, whereas the area of the vascular bundles was increased. ploidy analysis and the in situ expression patterns of histone h4 conf ...201727545518
genome-wide association study of eating and cooking qualities in different subpopulations of rice (oryza sativa l.).starch and protein are two major components of polished rice, and the amylose and protein contents affect eating and cooking qualities (ecqs). in the present study, genome-wide association study with high-quality re-sequencing data was performed for 10 ecqs in a panel of 227 non-glutinous rice accessions and four derived panels.201627544770
albino leaf 2 is involved in the splicing of chloroplast group i and ii introns in rice.chloroplasts play an essential role in plant growth and development through manipulating photosynthesis and the production of hormones and metabolites. although many genes or regulators involved in chloroplast biogenesis and development have been isolated and characterized, identification of novel components is still lacking. we isolated a rice (oryza sativa) mutant, termed albino leaf 2 (al2), using genetic screening. phenotypic analysis revealed that the al2 mutation caused obvious albino leav ...201627543605
accumulation and translocation of methylmercury and inorganic mercury in oryza sativa: an enriched isotope tracer study.methylmercury (mehg) accumulation in rice is an emerging human health issue, but uptake pathways and translocation into the grain remain poorly understood. we grew oryza sativa plants in pots of wetland soil amended with an enriched mercury isotope (94.3% 200hg) tracer, alongside unvegetated control pots, and assessed both ambient and tracer mehg and inorganic hg (ihg) concentrations in soil and plant tissues at three growth stages. based on similar ratios of ambient:tracer mehg concentrations i ...201727542632
overexpression of glycolate oxidase confers improved photosynthesis under high light and high temperature in rice.while glycolate oxidase (glo) is well known as a key enzyme for the photorespiratory metabolism in plants, its physiological function and mechanism remains to be further clarified. our previous studies have shown that suppression of glo in rice leads to stunted growth and inhibited photosynthesis (pn) which is positively and linearly correlated with decreased glo activities. it is, therefore, of interest to further understand whether pn can be improved when glo is up-regulated? in this study, fo ...201627540387
socio-economic characterisation of date palm (phoenix dactylifera l.) growers and date value chains in pakistan.increasing food production to feed its rapidly growing population is a major policy goal of pakistan. the production of traditional staples such as rice (oryza sativa l.) and bread wheat (triticum aestivum l.) has been intensified in many regions, but not in remote, drought-ridden areas. in these arid, marginal environments dates and their by-products are an option to complement staples given their high nutritive value and storability. to fill knowledge gaps about the role of date palm in the ho ...201627536506
whole-plant dynamic system of nitrogen use for vegetative growth and grain filling in rice plants (oryza sativa l.) as revealed through the production of 350 grains from a germinated seed over 150 days: a review and synthesis.a single germinated rice (oryza sativa l) seed can produce 350 grains with the sequential development of 15 leaves on the main stem and 7-10 leaves on four productive tillers (forming five panicles in total), using nitrogen (n) taken up from the environment over a 150-day growing season. nitrogen travels from uptake sites to the grain through growing organ-directed cycling among sequentially developed organs. over the past 40 years, the dynamic system for n allocation during vegetative growth an ...201627536309
extensive sequence divergence between the reference genomes of two elite indica rice varieties zhenshan 97 and minghui 63.asian cultivated rice consists of two subspecies: oryza sativa subsp. indica and o. sativa subsp. japonica despite the fact that indica rice accounts for over 70% of total rice production worldwide and is genetically much more diverse, a high-quality reference genome for indica rice has yet to be published. we conducted map-based sequencing of two indica rice lines, zhenshan 97 (zs97) and minghui 63 (mh63), which represent the two major varietal groups of the indica subspecies and are the parent ...201627535938
cooperation between the h3k27me3 chromatin mark and non-cg methylation in epigenetic regulation.h3k27me3 is a repressive chromatin mark of genes and is catalyzed by homologs of enhancer of zeste [e(z)], a component of polycomb-repressive complex 2 (prc2), while dna methylation that occurs in cg and non-cg (chg and chh, where h is a, c, or t) contexts is a hallmark of transposon silencing in plants. however, the relationship between h3k27me3 and dna methylation in gene repression remains unclear. in addition, the mechanism of prc2 recruitment to specific genes is not known in plants. here, ...201627535791
snp-based discovery of salinity-tolerant qtls in a bi-parental population of rice (oryza sativa).breeding for salt tolerance is the most promising approach to enhance the productivity of saline prone areas. however, polygenic inheritance of salt tolerance in rice acts as a bottleneck in conventional breeding for salt tolerance. hence, we set our goals to construct a single nucleotide polymorphism (snp)-based molecular map employing high-throughput snp marker technology and to investigate salinity tolerant qtls with closest flanking markers using an elite rice background. seedling stage sali ...201627535768
three zinc-finger rna-binding proteins in cabbage (brassica rapa) play diverse roles in seed germination and plant growth under normal and abiotic stress conditions.despite the increasing understanding of the stress-responsive roles of zinc-finger rna-binding proteins (rzs) in several plant species, such as arabidopsis thaliana, wheat (triticum aestivum) and rice (oryza sativa), the functions of rzs in cabbage (brassica rapa) have not yet been elucidated. in this study, the functional roles of the three rz family members present in the cabbage genome, designated as brrz1, brrz2 and brrz3, were investigated in transgenic arabidopsis under normal and environm ...201727528428
two novel antimicrobial defensins from rice identified by gene coexpression network analyses.defensins form an antimicrobial peptides (amp) family, and have been widely studied in various plants because of their considerable inhibitory functions. however, their roles in rice (oryza sativa l.) have not been characterized, even though rice is one of the most important staple crops that is susceptible to damaging infections. additionally, a previous study identified 598 rice genes encoding cysteine-rich peptides, suggesting there are several uncharacterized amps in rice. we performed in si ...201627527801
population structure analysis and association mapping of blast resistance in indica rice (oryza sativa l.) landraces.rice blast caused by magnaporthe oryzae is one of the most devastating rice diseases worldwide. to understand the genetic diversity of indica landrace accessions and identify simple sequence repeat (ssr) markers that are associated with blast resistance, a population of 276 indica landraces from across the world was constructed. this population was then used to evaluate the blast-resistance phenotype through artificial inoculation under controlled conditions in 2012 and 2013. the genetic diversi ...201627525942
photosynthesis enhanced oxidative stress tolerance in high-yield rice varieties (oryza sativa var. japonica l.) in the field.the objective of this study was to understand varietal differences in photosynthetic characteristics, chlorophyll fluorescence, antioxidant capability, and yield of japonica rice varieties. nanjing 44, oryza sativa var. japonica (average yield of 12.7 t/ha), nanjing 46, and nanjing 5055 (average yields of 11.3 and 11.5 t/ha) were included as "super" and high-yield varieties, respectively, whereas wuyunjing 7 (average yield of 10.2 t/ha) was included as a control variety. these varieties were gro ...201627525885
rapid changes in root hydraulic conductivity and aquaporin expression in rice (oryza sativa l.) in response to shoot removal - xylem tension as a possible signal.it is not clear how plants adjust the rate of root water uptake to that of shoot water loss. the aim of this study on rice was to test the idea that root aquaporins (aqps) and xylem tension play a role in this adjustment.201627524161
polar patterning of the spikelet is disrupted in the two opposite lemma mutant in rice.angiosperms produce diverse flowers and the pattern of floral symmetry is a major factor for flower diversification. bilaterally symmetric flowers have evolved multiple times in different angiosperm lineages from radially symmetric ancestors. whereas most monocots produce radially symmetric flowers, grasses such as rice (oryza sativa) and maize (zea mays) generate bilaterally symmetric flowers and spikelets. in this paper, we focused on the two opposite lemma (tol) mutant, which displays a pleio ...201727522958
enhanced salt tolerance under nitrate nutrition is associated with apoplast na+ content in canola (brassica. napus l.) and rice (oryza sativa l.) plants.to analyze the effect of nitrogen form on salt stress, we studied the response of two different plant species, canola (brassica napus l.), a dicotyledon which prefers no3(-) nutrition, and rice (oryza sativa l.), a monocotyledon which prefers nh4(+) nutrition, to salt stress under no3(-) (nn) and nh4(+) (an) nutrition. salt stress was simulated by the addition of 150 and 100 mm nacl to nn (nns) and an (ans) in canola and rice seedlings, respectively. salt stress induced reductions of shoot and r ...201627519313
evaluation of the role of the lysm receptor-like kinase, osnfr5/osrlk2 for am symbiosis in rice.in legume-specific rhizobial symbiosis, host plants perceive rhizobial signal molecules, nod factors, by a pair of lysm receptor-like kinases, nfr1/lyk3 and nfr5/nfp, and activate symbiotic responses through the downstream signaling components also required for arbuscular mycorrhizal (am) symbiosis. recently, the rice nfr1/lyk3 ortholog, oscerk1, was shown to play crucial roles for am symbiosis. on the other hand, the roles of the nfr5/nfp ortholog in rice have not been elucidated, while it has ...201627519312
ricebase: a breeding and genetics platform for rice, integrating individual molecular markers, pedigrees and whole-genome-based data.ricebase (http://ricebase.org) is an integrative genomic database for rice (oryza sativa) with an emphasis on combining datasets in a way that maintains the key links between past and current genetic studies. ricebase includes dna sequence data, gene annotations, nucleotide variation data and molecular marker fragment size data. rice research has benefited from early adoption and extensive use of simple sequence repeat (ssr) markers; however, the majority of rice ssr markers were developed prior ...201627515824
defence responses in rice plants in prior and simultaneous applications of cladosporium sp. during leaf blast suppression.an alternative method to control rice blast (magnaporthe oryzae) is to include biological agent in the disease management strategy. the objective of this study was to assess the leaf blast-suppressing effects of rice phylloplane fungi. one cladosporium sp. phylloplane fungus was shown to possess biocontrolling traits based on its morphological characteristics and an analysis of its 18s ribosomal dna. experiments aimed at determining the optimal time to apply the bioagent and the mechanisms invol ...201627515526
first comprehensive proteome analyses of lysine acetylation and succinylation in seedling leaves of brachypodium distachyon l.protein acetylation and succinylation are the most crucial protein post-translational modifications (ptms) involved in the regulation of plant growth and development. in this study, we present the first lysine-acetylation and lysine-succinylation proteome analysis of seedling leaves in brachypodium distachyon l (bd). using high accuracy nano lc-ms/ms combined with affinity purification, we identified a total of 636 lysine-acetylated sites in 353 proteins and 605 lysine-succinylated sites in 262 ...201627515067
post-flowering night respiration and altered sink activity account for high night temperature-induced grain yield and quality loss in rice (oryza sativa l.).high night temperature (hnt) is a major constraint to sustaining global rice production under future climate. physiological and biochemical mechanisms were elucidated for hnt-induced grain yield and quality loss in rice. contrasting rice cultivars (n22, tolerant; gharib, susceptible; ir64, high yielding with superior grain quality) were tested under control (23°c) and hnt (29°c) using unique field-based tents from panicle initiation till physiological maturity. hnt affected 1000 grain weight, gr ...201727513992
the exonuclease homolog osrad1 promotes accurate meiotic double-strand break repair by suppressing nonhomologous end joining.during meiosis, programmed double-strand breaks (dsbs) are generated to initiate homologous recombination, which is crucial for faithful chromosome segregation. in yeast, radiation sensitive1 (rad1) acts together with radiation sensitive9 (rad9) and hydroxyurea sensitive1 (hus1) to facilitate meiotic recombination via cell-cycle checkpoint control. however, little is known about the meiotic functions of these proteins in higher eukaryotes. here, we characterized a rad1 homolog in rice (oryza sat ...201627512017
effects of germination and high hydrostatic pressure processing on mineral elements, amino acids and antioxidants in vitro bioaccessibility, as well as starch digestibility in brown rice (oryza sativa l.).the effects of germination and high hydrostatic pressure (hhp) processing on the in vitro bioaccessibility of mineral elements, amino acids (aas), antioxidants and starch in brown rice (br) were investigated. germinated br (gbr) was obtained by incubating at 37°c for 36h and then subjected to hhp treatments at 0.1, 100, 300 and 500mpa for 10min. the in vitro bioaccessibility of calcium and copper was increased by 12.59-52.17% and 2.87-23.06% after hhp, respectively, but bioaccessible iron was de ...201727507507
nitrogen can improve the rapid response of photosynthesis to changing irradiance in rice (oryza sativa l.) plants.to identify the effect of nitrogen (n) nutrition on the dynamic photosynthesis of rice plants, a pot experiment was conducted under two n conditions. the leaf n and chlorophyll levels, as well as steady-state photosynthesis, were significantly increased under high n. after the transition from saturating to low light levels, decreases in the induction state (is%) of leaf photosynthesis (a) and stomatal conductance (gs) were more severe under low than under high n supply. after the transition from ...201627506927
the effect of periphyton on seed germination and seedling growth of rice (oryza sativa) in paddy area.periphyton is widely distributed in paddy fields and its interactions with paddy soil and rice growth have been reported rarely. in this study, model paddy ecosystems with different additional soil substrates were simulated under controlled conditions to investigate the effects of periphyton on rice seed germination and seedling growth. results show that the selected soil substrates had significant effects on the metabolic activities and growth of periphyton in paddy fields. the addition of stra ...201727503628
transcriptome profiling of drought responsive noncoding rnas and their target genes in rice.plant transcriptome profiling has provided a tool for understanding the mechanisms by which plants respond to stress conditions. analysis of genome-wide transcriptome will provides a useful dataset of drought responsive noncoding rnas and their candidate target genes that may be involved in drought stress responses.201627501838
molecular cloning of melatonin 3-hydroxylase and its production of cyclic 3-hydroxymelatonin in rice (oryza sativa).melatonin is metabolized in animals to cyclic 3-hydroxymelatonin (3-ohm) not by an enzymatic pathway, but by interaction with hydroxyl radicals. the production of 3-ohm in animals suggests the possible presence of 3-ohm in plants. prior to the identification of 3-ohm in plants, we directly cloned the corresponding gene(s) responsible for 3-ohm synthesis using escherichia coli library strains expressing genes belonging to the 2-oxoglutarate-dependent dioxygenase (2-odd) superfamily from rice. thr ...201627500558
domestication history and geographical adaptation inferred from a snp map of african rice.african rice (oryza glaberrima steud.) is a cereal crop species closely related to asian rice (oryza sativa l.) but was independently domesticated in west africa ∼3,000 years ago. african rice is rarely grown outside sub-saharan africa but is of global interest because of its tolerance to abiotic stresses. here we describe a map of 2.32 million snps of african rice from whole-genome resequencing of 93 landraces. population genomic analysis shows a population bottleneck in this species that began ...201627500524
rice (oryza sativa) laccases involved in modification and detoxification of herbicides atrazine and isoproturon residues in plants.atrazine (atr) and isoproturon (ipu) as herbicides have become serious environmental contaminants due to their overuse in crop production. although atr and ipu in soils are easily absorbed by many crops, the mechanisms for their degradation or detoxification in plants are poorly understood. this study identified a group of novel genes encoding laccases (ec 1.10.3.2) that are possibly involved in catabolism or detoxification of atr and ipu residues in rice. transcriptome profiling shows at least ...201627499219
a gene encoding pentatricopeptide repeat protein partially restores fertility in rt98-type cytoplasmic male-sterile rice.cytoplasmic male sterility (cms) lines in rice, which have the cytoplasm of a wild species and the nuclear genome of cultivated rice, are of value for the study of genetic interactions between the mitochondrial and nuclear genomes. the rt98-type cms line rt98a and the fertility restorer line rt98c carry the cytoplasm of the wild species oryza rufipogon and the nuclear genome of the taichung 65 cultivar (oryza sativa l.). based on a classical crossing experiment, fertility is reported to be resto ...201627498808
rice putative methyltransferase gene ostsd2 is required for root development involving pectin modification.pectin synthesis and modification are vital for plant development, although the underlying mechanisms are still not well understood. here, we report the functional characterization of the ostsd2 gene, which encodes a putative methyltransferase in rice. all three independent t-dna insertion lines of ostsd2 displayed dwarf phenotypes and serial alterations in different zones of the root. these alterations included abnormal cellular adhesion and schizogenous aerenchyma formation in the meristematic ...201627497286
selenate mitigates arsenite toxicity in rice (oryza sativa l.) by reducing arsenic uptake and ameliorates amino acid content and thiol metabolism.arsenic (as) is a toxic element with the potential to cause health effects in humans. besides rice is a source of both amino acids (aas) and mineral nutrients, it is undesired source of as for billions of people consuming rice as the staple food. selenium (se) is an essential metalloid, which can regulate as toxicity by strengthening antioxidant potential. the present study was designed to investigate as(iii) stress mitigating effect of se(vi) in rice. the level of as, thiolic ligands and aas wa ...201627497079
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