Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| action pattern of valencia orange pme de-esterification of high methoxyl pectin and characterization of modified pectins. | valencia pectinmethylesterase (pme) fractions, b-pme, containing 36 and 13 kda protein bands and u-pme, containing a 36 and 27 kda protein bands, were used to de-esterify original pectin (o-pec) from 73% degree of esterification (%de) to 63% (b-pec) and 61% de (u-pec), respectively. most o-pec eluted from ion exchange chromatography at low salt concentration and a smaller component eluted at higher ionic strength. b-pec and u-pec eluted as one broad peak at higher ionic strength. pme modificatio ... | 2005 | 16216228 |
| biological activity of carotenoids in red paprika, valencia orange and golden delicious apple. | carotenoid fractions were extracted from red paprika, valencia orange peel and the peel of golden delicious apple. thus, hypophasic carotenoids of paprika (pm1), orange (pm3) and apple (pm4), and epiphasic extractions of paprika (pm2) and apple (pm5) were obtained by extraction, saponification and partition between meoh-h(2)o (9:1) (hypophasic) and hexane (epiphasic). a high content of capsanthin was quantified in hypophasic carotenoids (pm1) from red spice paprika, whereas the hypophasic fracti ... | 2005 | 16177974 |
| soil water repellency induced by long-term irrigation with treated sewage effluent. | this study describes soil water repellency developed under prolonged irrigation with treated sewage effluent in a semiarid environment. soil surface layer (0-5 cm) and soil profile (0-50 cm) transects were sampled at a high resolution at the close of the irrigation season and rainy winter season. samples from 0- to 5-cm transects were subdivided into 1-cm slices to obtain fine scale resolution of repellency and organic matter distribution. extreme to severe soil water repellency in the 0- to 5-c ... | 2005 | 16151242 |
| lycopene-epsilon-cyclase pre-mrna is alternatively spliced in cara cara navel orange (citrus sinensis osbeck). | lycopene-epsilon-cyclase is one of the key enzymes related to alpha-carotene metabolism in plants. a full-length cdna of 1300 bp encoding lycopene-epsilon-cyclase (lyce) was generated from cara cara navel orange, a unique navel orange containing both lycopene and beta-carotene in its pulp, with little or no alpha-carotene. the gene had a 14 bp nucleotides deletion and caused a terminal mutation. dna sequence corresponding to the deletion region revealed that two repeats of 6 bp (aggtgt) were fla ... | 2005 | 16086259 |
| [creation and hybrid identification of the sexual progenies using citrus somatic hybrid as pollen parent]. | 110 plantlets were obtained from the cross between citrus allotetraploid somatic hybrid [hamlin sweet orange (citrus sinensis osback) + rough lemon (c. jambhiri luss)] (as the pollen parent) and mono-embryonic diploid type iben no.4 [huanongbendizao tangerine (c. reticulata blanco) x ichang papeda (c. ichangensis swingle)] by the means of young embryo culture in vitro, and there were 93 triploids and 17 diploids through chromosome counting and the ploidy analyser identification. rapd analyses sh ... | 2005 | 16044915 |
| analysis of flavanone-7-o-glycosides in citrus juices by short-end capillary electrochromatography. | the separation of the major flavanone-7-o-glycoside constituents of citrus was carried out by isocratic reversed phase capillary electrochromatography using a 75 microm i.d. silica fused column packed with 5 microm ods silica gel. in comparison to hplc mode, capillary electrochromatography resolution of flavanone glycosides was obtained with a high selectivity factor. optimum separation conditions were found using a mixture of ammonium formate (ph 2.5)--acetonitrile (8:2, v/v) as the mobile phas ... | 2005 | 16013605 |
| [control effects of mikania micrantha alcohol extract on citrus red mite panonychus citri]. | this study showed that spraying 0.2 and 0.4 g x l(-1) mikania micrantha alcohol extracts on panonychus citri could significantly decrease the survival rates of p. citri eggs, larvae and nymphs, and 0.1 g x l(-1) m. micrantha alcohol extract could also significantly decrease the survival rates of larvae and nymphs. in a no-choice test, both the fecundity and the longevity of female p. citri fed on the leaves treated with all three test concentrations 0.1, 0.2 and 0.4 g x l(-1) of m. micrantha alc ... | 2005 | 16011182 |
| nobiletin: efficient and large quantity isolation from orange peel extract. | it is known that nobiletin possesses anticancer, antiviral and anti-inflammatory activities. recently, the demand for nobiletin in large quantities and high purity has increased. however, conventional normal-phase silica gel chromatography and c(18)-reverse-phase separation methods cannot satisfy the requirement of pure and gram scale nobiletin in a timely manner. in exploring the composition and the biological activities of polymethoxyflavones from sweet orange (citrus sinensis) peel, we develo ... | 2006 | 15999338 |
| involvement of ethylene biosynthesis and perception in the susceptibility of citrus fruits to penicillium digitatum infection and the accumulation of defence-related mrnas. | citrus fruits infected with the fungus penicillium digitatum substantially increase the production of the plant hormone ethylene. in this study, the regulation of ethylene biosynthesis in citrus sinensis-infected fruits and its putative involvement in an active defence response against p. digitatum infection is examined. ethylene production is demonstrated as being the result of the co-ordinated and differential up-regulation of at least three ethylene biosynthetic genes: acs1, acs2, and aco. bl ... | 2005 | 15983011 |
| [changes in polyamine levels in citrus sinensis osb. cv. valencia callus during somatic embryogenesis]. | somatic embryogenetic capability and changes in polyamine level and their relationship were analyzed using the long-term (8 years) subcultured calli of citrus sinensis osb. cv. valencia as materials. the results showed that endogenous polyamine contents in embryogenic calli were higher than those in non-embryogenic calli, and the embryogenetic capability was positively correlated to the levels of endogenous polyamines. when the calli were transferred to a differentiation medium, the putrescine c ... | 2005 | 15961902 |
| antioxidant activity of the flavonoid hesperidin in chemical and biological systems. | the antioxidant hesperidin, a major flavonoid in sweet orange and lemon, was evaluated using chemical and biological systems. the chemical assay evaluates the hesperidin capacity to sequester 1,1-diphenyl-2-picrylhydrazyl (dpph*). biological studies were done using the eukaryotic cells of superoxide-dismutase proficient and deficient strains of saccharomyces cerevisiae treated with hesperidin and the stressing agents hydrogen peroxide or paraquat (methylviologen; 1,1'-dimethyl-4,4'-bipyridinium ... | 2005 | 15941311 |
| g-protein-coupled alpha2a-adrenoreceptor agonists differentially alter citrus leaf and fruit abscission by affecting expression of acc synthase and acc oxidase. | temporal and spatial expression patterns of genes encoding 1-aminocyclopropane-1-carboxylate (acc) synthase (acs1 and acs2) and acc oxidase (aco), acc concentration, and ethylene production in leaves and fruit of 'valencia' orange (citrus sinensis [l.] osbeck) were examined in relation to differential abscission after treatment with 2-chloroethylphosphonic acid (ethephon) alone or in combination with guanfacine or clonidine, two g-protein-coupled alpha(2a)-adrenoreceptor selective agonists. guan ... | 2005 | 15928018 |
| toward validation of process criteria for high-pressure processing of orange juice with predictive models. | mathematical models were developed to predict time to inactivation (tti) by high-pressure processing of salmonella in australian valencia orange juice (ph 4.3) and navel orange juice (ph 3.7) as a function of pressure magnitude (300 to 600 mpa) and inoculum level (3 to 7 log cfu/ml). for each model, the tti was found to increase with increasing inoculum level and decrease with increasing pressure magnitude. the u.s. food and drug administration juice hazard analysis and critical control point re ... | 2005 | 15895726 |
| protoplast transformation and regeneration of transgenic valencia sweet orange plants containing a juice quality-related pectin methylesterase gene. | valencia orange [citrus sinensis (l.) osbeck] is the leading commercial citrus species in the world for processed juice products; however, the presence of thermostable pectin methylesterase (tspme) reduces its juice quality. a long-term strategy of this work is to eliminate or greatly reduce tspme activity in valencia orange. previous work resulted in the isolation of a putative tspme gene, cspme4, associated with a thermostable protein fraction of valencia orange juice. to begin research design ... | 2005 | 15875190 |
| effect of certain bioactive plant extracts on clinical isolates of beta-lactamase producing methicillin resistant staphylococcus aureus. | ethanolic extracts and some fractions from 10 indian medicinal plants, known for antibacterial activity, were investigated for their ability to inhibit clinical isolates of beta-lactamase producing methicillin-resistant staphylococcus aureus (mrsa) and methicillin-sensitive s. aureus (mssa). synergistic interaction of plant extracts with certain antibiotics was also evaluated. the mrsa test strains were found to be multi-drug resistant and also exhibited high level of resistance to common beta-l ... | 2005 | 15812867 |
| relationships between nutrient composition of flowers and fruit quality in orange trees grown in calcareous soil. | to determine if flower nutrient composition can be used to predict fruit quality, a field experiment was conducted over three seasons (1996-1999) in a commercial orange orchard (citrus sinensis (l.) osbeck cv. 'valencia late', budded on troyer citrange rootstock) established on a calcareous soil in southern portugal. flowers were collected from 20 trees during full bloom in april and their nutrient composition determined, and fruits were harvested the following march and their quality evaluated. ... | 2005 | 15805096 |
| a set of primers for analyzing chloroplast dna diversity in citrus and related genera. | chloroplast simple sequence repeat (cpssr) markers in citrus were developed and used to analyze chloroplast diversity of citrus and closely related genera. fourteen cpssr primer pairs from the chloroplast genomes of tobacco (nicotiana tabacum l.) and arabidopsis were found useful for analyzing the citrus chloroplast genome (cpdna) and recoded with the prefix spcc (ssr primers for citrus chloroplast). eleven of the 14 primer pairs revealed some degree of polymorphism among 34 genotypes of citrus, ... | 2005 | 15805086 |
| transcription analysis of pils and xpsel genes from xylella fastidiosa. | xylella fastidiosa is a xylem-limited phytopathogen responsible for diseases in several plants such as citrus and coffee. analysis of the bacterial genome revealed some putative pathogenicity-related genes that could help to elucidate the molecular mechanisms of plant-pathogen interactions. in the present work, the transcription of three genes of the bacterium, grown in defined and rich media and also in media containing host plant extracts (sweet orange, 'ponkan' and coffee) was analyzed by rt- ... | 2005 | 15803391 |
| citrus huanglongbing in são paulo state, brazil: pcr detection of the 'candidatus' liberibacter species associated with the disease. | symptoms of huanglongbing (hlb), one of the most serious diseases of citrus in asia and africa, have been noticed in march 2004 in the araraquara region of são paulo state, brazil. hlb has not been reported previously from america. the causal hlb bacteria, candidatus liberibacter africanus in africa and candidatus liberibacter asiaticus in asia, can be detected in symptomatic citrus leaves by pcr amplification of their 16s rdna with previously described primers. when this technique was applied t ... | 2005 | 15797817 |
| isolation, characterization, and pectin-modifying properties of a thermally tolerant pectin methylesterase from citrus sinensis var. valencia. | the thermally tolerant pectin methylesterase (tt-pme) was isolated as a monocomponent enzyme from sweet orange fruit (citrus sinensis var. valencia). it was also isolated from flower and vegetative tissue. the apparent molecular weight of fruit tt-pme was 40800 by sds-page and the isoelectric point estimated as pi 9.31 by ief-page. maldi-tof ms identified no tryptic-peptide ions from tt-pme characteristic of previously described citrus pmes. tt-pme did not absolutely require supplemented salt fo ... | 2005 | 15769165 |
| leaf volatile compounds of six citrus somatic allotetraploid hybrids originating from various combinations of lime, lemon, citron, sweet orange, and grapefruit. | volatile compounds were extracted by a pentane/ether (1:1) mixture from the leaves of six citrus somatic allotetraploid hybrids resulting from various combinations of lime, lemon, citron, sweet orange, and grapefruit. extracts were examined by gas chromatography-mass spectrometry (gc-ms) and compared with those of their respective parents. all hybrids having an acid citrus parent exhibit the same relative contents in hydrocarbons and oxygenated compounds as the acid citrus, while the (grapefruit ... | 2005 | 15769160 |
| varietal and interspecific influence on micronutrient contents in citrus from the mediterranean area. | to specify the genotypic variation of mediterranean citrus juices, the contents of carotenoids, flavonoids, and vitamin c were determined by high-performance liquid chromatography. a selection of orange varieties and mandarin species from the mediterranean area (citrus sinensis, citrus deliciosa ten, and citrus clementina hort. ex tan) was evaluated using carotenoid profiles and flavanones contents. among the eight varieties of orange (salustiana, hamlin, shamouti, pera, valencia, maltaise, sang ... | 2005 | 15769147 |
| the climacteric-like behaviour of young, mature and wounded citrus leaves. | although leaves and other vegetative tissues are generally considered as non-climacteric, citrus leaves show a climacteric system ii behaviour after detachment. upon harvest, young, fully expanded 'valencia' orange (citrus sinensis) leaves ( approximately 60-d-old) exhibited two phases of ethylene production. the first phase, up to 6 d after detachment, was characterized by a low and constant ethylene production (system i pathway), associated with a constitutive expression of acc synthase 2 (csa ... | 2005 | 15767320 |
| establishment of a genetically marked insect-derived symbiont in multiple host plants. | alcaligenes xylosoxidans subsp. denitrificans, originally isolated from the cibarial region of the foregut of the glassy-winged sharpshooter (homalodisca coagulata), was transformed using the himar1 transposition system to express egfp. seedlings of six potential host plants were inoculated with transformed bacteria and 2 weeks later samples were taken 5 cm away and analyzed by quantitative real-time pcr using primers designed to amplify the gene insert. the largest colony of 3,591,427 cells/2 c ... | 2005 | 15723145 |
| starter substrate specificities of wild-type and mutant polyketide synthases from rutaceae. | chalcone synthases (chss) and acridone synthases (acss) belong to the superfamily of type iii polyketide synthases (pkss) and condense the starter substrate 4-coumaroyl-coa or n-methylanthraniloyl-coa with three malonyl-coas to produce flavonoids and acridone alkaloids, respectively. acss which have been cloned exclusively from ruta graveolens share about 75-85% polypeptide sequence homology with chss from other plant families, while 90% similarity was observed with chss from rutaceae, i.e., r. ... | 2005 | 15680984 |
| metal-binding proteins scanning and determination by combining gel electrophoresis, synchrotron radiation x-ray fluorescence and atomic spectrometry. | in the present work, protein bands from in vitro embriogenic callus (citrus sinensis l. osbeck) were investigated using micro-synchrotron radiation x-ray fluorescence (musr-xrf) after sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) separation. metal-binding protein quantification was done after microwave oven decomposition of gel by synchrotron radiation total reflection x-ray fluorescence (sr-txrf), flame atomic absorption spectrometry (faas) and flame atomic emission spect ... | 2005 | 15680280 |
| inotropic effect of citrus sinensis (l.) osbeck leaf extracts on the guinea pig atrium. | the objective of the present investigation was to determine the contractile effect of crude and acetone leaf extracts of citrus sinensis (l.) osb. on mammalian myocardium. crude leaf extracts have been used in folk medicine to treat neurological disorders. some flavonoids isolated from this plant presented a positive inotropic effect on myocardium. this motivated us to test the extracts on the atria of guinea pigs of both sexes (300-500 g) and surprisingly we observed inotropic depression instea ... | 2005 | 15665997 |
| effect of nigerian citrus (citrus sinensis osbeck) honey on ethanol metabolism. | the effect of nigerian citrus (citrus sinensis osbeck) honey on ethanol metabolism was tested using 45 consenting individuals in apparent good health and between the ages of 25 and 35 years. the subjects were moderate social drinkers matched in terms of body weight and build. the results obtained showed that on average, honey significantly (p < 0.05) increased the blood ethanol clearance rate by 68% and decreased the intoxication period by 43%, but insignificantly (p > 0.05) reduced the degree o ... | 2004 | 15662998 |
| repellency and toxicity of aromatic plant extracts against the mosquito culex pipiens molestus (diptera: culicidae). | the insecticidal activities of essential oil extracts from leaves, flowers and roots of aromatic plants against fourth-instar larvae of the mosquito culex pipiens molestus forskal were determined. extracts of foeniculum vulgare mill were the most toxic, followed by those of ferula hermonis boiss, citrus sinensis osbeck, pinus pinea l, laurus nobilis l and eucalyptus spp with lc50 values of 24.5, 44.0, 60.0, 75.0, 117.0 and 120.0 mg litre(-1), respectively. combination tests between the lc50 and ... | 2005 | 15662650 |
| identification and aroma impact of norisoprenoids in orange juice. | four norisoprenoids, alpha-ionone, beta-ionone, beta-cyclocitral, and beta-damascenone, along with their putative carotenoid precursors, were identified in valencia orange juice using time-intensity gc-o, gc-ms, and photodiode array hplc. alpha-ionone and beta-cyclocitral are reported in orange juice for the first time. gc-o aroma peaks were categorized into seven groups with similar sensory qualities: citrus/minty, metallic/mushroom/geranium, roasted/cooked/meaty/spice, fatty/soapy/green, sulfu ... | 2005 | 15656678 |
| cover crops and natural vegetation mulch effect achieved by mechanical management with lateral rotary mower in weed population dynamics in citrus. | the aim of this study was to obtain information on practical weed management in order to reduce the use of herbicides thereby contributing to the sustainable development of citrus crop. the experiment was carried out under field conditions at the experimental area of the department of vegetal production at the college of agriculture "luiz de queiroz," piracicaba, sp, brazil, during the season 2002-2003. influence of mulches produced by four types of vegetations on the dynamic population of weeds ... | 2005 | 15656179 |
| characterization of anthocyanins and pyranoanthocyanins from blood orange [citrus sinensis (l.) osbeck] juice. | anthocyanins from blood orange [citrus sinensis (l.) osbeck] juices were isolated and purified by means of high-speed countercurrent chromatography and preparative high-performance liquid chromatography. structures of the pigments were then elucidated by electrospray ionization multiple mass spectrometry and nuclear magnetic resonance (nmr) spectroscopy. the major anthocyanins of the juice were characterized as cyanidin 3-glucoside and cyanidin 3-(6"-malonylglucoside). furthermore, six minor ant ... | 2004 | 15563216 |
| analysis of antioxidant compounds in sweet orange peel by hplc-diode array detection-electrospray ionization mass spectrometry. | hplc-diode array detection-electrospray ionization mass spectrometry was used to determine qualitatively and quantitatively the flavonoid content of several fractions and residues of extracts of greek navel sweet orange peel (citrus sinensis) from the region of southern greece (leonidi-tripoli). the main groups of flavonoids found according to hplc retention times, spectral data and literature references were polymethoxylated flavones, c-glycosylated flavones, o-glycosylated flavones, o-glycosyl ... | 2005 | 15515108 |
| biochemical and molecular analysis of carotenoid biosynthesis in flavedo of orange (citrus sinensis l.) during fruit development and maturation. | changes in carotenoid content and composition and expression of carotenoid biosynthetic genes were analyzed in the flavedo of sweet orange (citrus sinensis l. osbeck, cv. navelate) fruit during development and maturation. lutein and all-e-violaxanthin were the major carotenoids in chloroplast-containing tissues. during fruit coloration, phytoene, beta-cryptoxanthin, zeaxanthin, and mainly (9z)-violaxanthin progressively accumulated, and a large proportion of apocarotenoids was also found in the ... | 2004 | 15506808 |
| a new potato virus in a new lineage of picorna-like viruses. | on constructing a cdna library for potato, 'contaminating' sequences with a significant identity to apple latent spherical virus (alsv) were found. determination of the remaining genome sequence indicated the presence of a bipartite virus with an rna1 and 2 of 7034 and 3315 nucleotides, respectively, excluding a poly(a)tail. rna1 encodes a single polyprotein (233 kda) and shares highest amino acid identity with alsv at 65%. conserved amino acid motifs typical for helicase, protease and rna-depen ... | 2004 | 15503203 |
| impact of genetically modified enterobacter cloacae on indigenous endophytic community of citrus sinensis seedlings. | enterobacter cloacae (strain pr2/7), a genetically modified endophyte (gme) in citrus plants, carrying different plasmids (pec3.0/18, pcele, pegla and pgfp), was inoculated into citrus sinensis seedlings under greenhouse conditions. the impact of this on the indigenous bacterial endophytic community was studied by analyses of 2 different morphologic groups. the germination rates of inoculated seeds were evaluated in greenhouse, and plasmid stability under in vitro conditions. results demonstrate ... | 2004 | 15459643 |
| expression of green fluorescent protein in xylella fastidiosa is affected by passage through host plants. | xylella fastidiosa, a gram-negative bacterial plant pathogen, causes pierce's disease of grapevine in north america. in south america the pathogen causes citrus variegated chlorosis, which is widespread in brazil. we have introduced into xylella fastidiosa a mini-tn5 transposon that encodes a green fluorescent protein (gfp) gene optimized for expression in bacteria. the mini-tn5 derivative was inserted into different sites of the genome in independent transconjugants as determined by southern bl ... | 2004 | 15386107 |
| genetic transformation of mature citrus plants. | most woody fruit species have long juvenile periods that drastically prolong the time required to analyze mature traits. evaluation of characteristics related to fruits is a requisite to release any new variety into the market. because of a decline in regenerative and transformation potential, genetic transformation procedures usually employ juvenile material as the source of plant tissue, therefore resulting in the production of juvenile plants. direct transformation of mature material could en ... | 2005 | 15310921 |
| interstock-induced mechanism of increased growth and salt resistance of orange (citrus sinensis) trees. | interstocks improve the growth and salt resistance of lemon (citrus limon (l.) burm. f.) trees, but their effects on orange (citrus sinensis (l.) osbeck) trees are unknown. we grew 'cleopatra' mandarin (cm) seedlings, budded trees of 'salustiano' orange (sao) on cm, 'valencia late' orange (vlo) on cm (vlo/cm), and interstock trees vlo/sao/cm in pots of sand watered with nutrient solution containing 5 (control) or 50 mm nacl for 12 weeks. plants were harvested on six successive occasions and the ... | 2004 | 15294757 |
| introduction of a citrus blight-associated gene into carrizo citrange [citrus sinensis (l.) osbc. x poncirus trifoliata (l.) raf.] by agrobacterium-mediated transformation. | the protein p12 accumulates in leaves of trees with citrus blight (cb), a serious decline of unknown cause. the function of p12 is not known, but sequence analysis indicates it may be related to expansins. in studies to determine the function of p12, sense and antisense constructs were used to make transgenic carrizo citrange using an agrobacterium-mediated transformation system. homogeneous beta-glucuronidase+ (gus+) sense and antisense transgenic shoots were regenerated using kanamycin as a se ... | 2004 | 15248084 |
| the salt-stress signal transduction pathway that activates the gpx1 promoter is mediated by intracellular h2o2, different from the pathway induced by extracellular h2o2. | several genes encoding putative glutathione peroxidase have been isolated from a variety of plants, all of which show the highest homology to the phospholipid hydroperoxide isoform. several observations suggest that the proteins are involved in biotic and abiotic stress responses. previous studies on the regulation of gpx1, the citrus sinensis gene encoding phospholipid hydroperoxide isoform, led to the conclusion that salt-induced expression of gpx1 transcript and its encoded protein is mediate ... | 2004 | 15247370 |
| isolation and characterization of a terminal flower homolog and its correlation with juvenility in citrus. | terminal flower is a key regulator of floral timing in arabidopsis and other herbaceous species. a homolog of this gene, cstfl, was isolated from the hybrid perennial tree crop washington navel orange (citrus sinensis l. osbeck). the deduced amino acid sequence of cstfl was 65% identical to the arabidopsis tfl1 protein. wild-type arabidopsis plants ectopically expressing cstfl showed late-flowering phenotypes similar to those described for overexpression of arabidopsis tfl1. in addition, the 35s ... | 2004 | 15235113 |
| nitrate improves growth in salt-stressed citrus seedlings through effects on photosynthetic activity and chloride accumulation. | we analyzed the effects of nitrate availability on growth of navelina (citrus sinensis (l.) osbeck) scions grafted on three citrus rootstocks differing in salt tolerance: carrizo citrange (citrus sinensis (l.) osbeck x poncirus trifoliata (l.) raf.), citrus macrophylla wester and cleopatra mandarin (citrus reshni hort. ex tanaka). salt stress reduced total plant biomass by 27-38%, whereas potassium nitrate supplementation partially counteracted this effect by increasing dry matter and new leaf a ... | 2004 | 15234900 |
| a new virus related to satsuma dwarf virus: the nucleotide sequence of the 3'-terminal regions of hyuganatsu virus rnas 1 and 2. brief report. | the sequences of the 3'-terminal 1306 and 2160 nucleotides of rnas 1 and 2 of a virus serologically related to satsuma dwarf virus (sdv) from hyuganatsu ( citrus tamurana hort. ex tan.) were determined, respectively. we found that the partial rna-dependent rna polymerase region in rna1 and the coat proteins (cps) region in rna2 of the virus tentatively named hyuganatsu virus (hv) have 78.3-84.0% and 76.9-80.7% amino acid sequence identities to those of known sdv-related viruses (sdv-rvs), i.e., ... | 2004 | 15221545 |
| a beta-galactosidase gene is expressed during mature fruit abscission of 'valencia' orange (citrus sinensis). | beta-galactosidases have been detected in a wide range of plants and are characterized by their ability to hydrolyse terminal non-reducing beta-d-galactosyl residues from beta-d-galactosides. these enzymes have been detected in a wide range of plant organs and tissues. in a search for differentially expressed genes during the abscission process in citrus, sequences encoding beta-galactosidase were identified. three cdna fragments of a beta-galactosidase gene were isolated from a cdna subtraction ... | 2004 | 15208347 |
| interaction between endophytic bacteria from citrus plants and the phytopathogenic bacteria xylella fastidiosa, causal agent of citrus-variegated chlorosis. | to isolate endophytic bacteria and xylella fastidiosa and also to evaluate whether the bacterial endophyte community contributes to citrus-variegated chlorosis (cvc) status in sweet orange (citrus sinensis [l.] osbeck cv. pera). | 2004 | 15189288 |
| potent inhibition by star fruit of human cytochrome p450 3a (cyp3a) activity. | there has been very limited information on the capacities of tropical fruits to inhibit human cytochrome p450 3a (cyp3a) activity. thus, the inhibitory effects of tropical fruits on midazolam 1'-hydroxylase activity of cyp3a in human liver microsomes were evaluated. eight tropical fruits such as common papaw, dragon fruit, kiwi fruit, mango, passion fruit, pomegranate, rambutan, and star fruit were tested. we also examined the inhibition of cyp3a activity by grapefruit (white) and valencia orang ... | 2004 | 15155547 |
| changes in the levels of polymethoxyflavones and flavanones as part of the defense mechanism of citrus sinensis (cv. valencia late) fruits against phytophthora citrophthora. | phytophthora citrophthora causes serious losses in citrus fruits through brown rot lesion. the effect of infection with p. citrophthora on citrus sinensis (cv. valencia late) fruits was studied, with particular reference to the levels of the flavanones hesperidin and isonaringin and the polymethoxyflavones sinensetin, nobiletin, tangeretin, and heptamethoxyflavone, because flavonoids are most probably involved as natural defense or resistance mechanisms in this genus. changes in the levels of th ... | 2004 | 15053528 |
| estimation of the number of aphids carrying citrus tristeza virus that visit adult citrus trees. | aphid species were counted on citrus trees in orchards in valencia, spain, in the spring and autumn of 1997, 1998 and 1999. moericke yellow water traps, the 'sticky shoot' method and counts of established colonies were used in extensive surveys in which 29,502 aphids were recorded and identified. aphis spiraecola and aphis gossypii were the most abundant aphid species. the numbers of aphid species landing on mature trees of grapefruit, sweet orange, lemon and clementine and satsuma mandarins, we ... | 2004 | 15036840 |
| isolation of a dehydrin cdna from orange and grapefruit citrus fruit that is specifically induced by the combination of heat followed by chilling temperatures. | dehydrins (dhns; late embryogenesis abundant d-11) are a family of plant proteins induced in response to environmental stresses such as water stress, salinity and freezing or which occur during the late stages of embryogenesis. previously, it was reported that citrus contains a small gene family encoding a unique class of dehydrins that differs from most other plant dehydrins in various respects, such as having an unusual k-segment similar to that of gymnosperms. in the present study, we identif ... | 2004 | 15032860 |
| production of intergeneric somatic hybrids between round kumquat (fortunella japonica swingle) and 'morita navel' orange (citrus sinensis osbeck). | intergeneric somatic hybrids between embryogenic callus-derived protoplasts of round kumquat (fortunella japonica swingle) and 'morita navel' orange (citrus sinensis osbeck) were produced by electrofusion. among the eight different fusion strains obtained, six showed normal morphology, whereas the remaining two showed malformation. all the regenerated plants were intermediate in leaf morphology and had thick and round leaves, which are typical characteristics of polyploids. ploidy analyses by fl ... | 2004 | 14997338 |
| adult diet and male-female contact effects on female reproductive potential in mexican fruit fly (anastrepha ludens loew) (diptera tephritidae). | wild strains of fruit flies (diptera: tephritidae) placed into laboratory rearing conditions are subjected to selection pressures caused by the diet, cages, density of flies, and other factors. selection that changes mating behavior of the strain may result in less effective males released in sterile insect programs. tests were performed to examine the effects of protein in diet and adult interactions on egg production and mating during sexual maturation of the mexican fruit fly (anastrepha lude ... | 2003 | 14994799 |
| nonhost status of citrus sinensis cultivar valencia and c. paradisi cultivar ruby red to mexican anastrepha fraterculus (diptera: tephritidae). | anastrepha fraterculus (wiedemann) is recognized as a pest of citrus, apples, and blackberries in south america. in mexico, it is mainly found in fruit of the family myrtaceae and has never been reported infesting citrus. here, we sought to determine whether females stemming from mexican a. fraterculus populations (collected in the state of veracruz) would lay eggs in 'valencia' oranges and 'ruby red' grapefruit and, if so, whether larvae would hatch and develop. we worked under laboratory and s ... | 2003 | 14977105 |
| application of mri to monitor the process of ripening and decay in citrus treated with chitosan solutions. | chitosan is a biopolymer, which has numerous applications in agriculture and agroindustries. coating fruit and vegetables with chitosan has some positive advantages for the long-term storage of foods, because the film of chitosan provides a kind of an active package, which allows a gradual release of preservatives, thus inhibiting fungal growth and maintaining the external appearance of the fruit for a longer time in this study, two varieties of citrus, fortune mandarins and valencia oranges wer ... | 2004 | 14972402 |
| constitution of the juice lipids of the florida valencia orange (citrus sinensis l.). | 2016 | 14840502 | |
| induction of apoptosis by citrus paradisi essential oil in human leukemic (hl-60) cells. | limonene is a primary component of citrus essential oils (eos) and has been reported to induce apoptosis on tumor cells. little is known about induction of apoptosis by citrus eos. in this study, we examined induction of apoptosis by citrus aurantium var. dulcis (sweet orange) eo, citrus paradisi (grapefruit) eo and citrus limon (lemon) eo. these eos induced apoptosis in hl-60 cells and the apoptosis activities were related to the limonene content of the eos. moreover, sweet orange eo and grapef ... | 2003 | 14758720 |
| citrus leprosis and its status in florida and texas: past and present. | according to published reports from 1906 to 1968, leprosis nearly destroyed the florida citrus industry prior to 1925. this was supported with photographs showing typical leprosis symptoms on citrus leaves, fruit, and twigs. support for the past occurrence of citrus leprosis in florida includes: (1) presence of twig lesions in affected orange blocks in addition to lesions on fruits and leaves and corresponding absence of similar lesions on grapefruit; (2) yield reduction and die-back on infected ... | 2003 | 14756416 |
| brevipalpus californicus, b. obovatus, b. phoenicis, and b. lewisi (acari: tenuipalpidae): a review of their biology, feeding injury and economic importance. | the genus brevipalpus includes most of the economically important species of tenuipalpidae. many brevipalpus species reproduce by theletokous parthenogenesis while other species reproduce by male fertilization of female eggs. previous researchers have determined that brevipalpus californicus (banks), b. obovatus donnadieu, and b. phoenicis (geijskes) females were haploid with two chromosomes. the life cycle and developmental times for these three species are reviewed. longevity of each brevipalp ... | 2003 | 14756411 |
| accumulation of carotenoids and expression of carotenoid biosynthetic genes during maturation in citrus fruit. | the relationship between carotenoid accumulation and the expression of carotenoid biosynthetic genes during fruit maturation was investigated in three citrus varieties, satsuma mandarin (citrus unshiu marc.), valencia orange (citrus sinensis osbeck), and lisbon lemon (citrus limon burm.f.). we cloned the cdnas for phytoene synthase (citpsy), phytoene desaturase (citpds), zeta-carotene (car) desaturase (citzds), carotenoid isomerase (citcrtiso), lycopene beta-cyclase (citlcyb), beta-ring hydroxyl ... | 2004 | 14739348 |
| effects of 15n application frequency on nitrogen uptake efficiency in citrus trees. | two irrigation systems were used to compare nitrogen uptake efficiency in citrus trees and to evaluate the no3- runoff in "navelina" orange trees [citrus sinensis (l.) osbeck] on carrizo citrange rootstock (citrus sinensis x poncirus trifoliata raf.). these were fertilized with 125 g n as labelled k15no3 and grown outdoors in containers filled with a sand-loamy soil. two groups of 3 trees received this n dose either in five equally split applications by a flooding irrigation system or in 66 appl ... | 2003 | 14717433 |
| the dwarfing mechanism of citrus rootstocks f&a 418 and #23 is related to competition between vegetative and reproductive growth. | the annual development of navelina (citrus sinensis (l.) osbeck) trees budded on three hybrid citrus rootstocks was studied. two rootstocks, named #23 and #24, were obtained from the cross of troyer citrange (c. sinensis x poncirus trifoliata (l.) raf.) x cleopatra mandarin (c. reshni hort. ex tan.). the third rootstock, named f&a 418, came from a cross of troyer citrange x common mandarin (c. deliciosa ten.). rootstocks #23 and f&a 418 are dwarfing rootstocks and reduce the size of the scion by ... | 2004 | 14676038 |
| gus gene remains stable in transgenic citrus callus recovered from cryopreservation. | the conservation of transgenic materials is very important, paticularly for their potential future use in crop development. in this study, transgenic callus cultures of 'newhall' navel orange (citrus sinensis osbeck) were cryopreserved by a vitrification method. transgenic calluses survived cryopreservation and recovered under normal culture conditions. the results of pcr (polymerase chain reaction) amplification, southern blotting and sscp (single-strand conformation polymorphism) assay showed ... | 2006 | 14671689 |
| a new abscisic acid catabolic pathway. | we report the discovery of a new hydroxylated abscisic acid (aba) metabolite, found in the course of a mass spectrometric study of aba metabolism in brassica napus siliques. this metabolite reveals a previously unknown catabolic pathway for aba in which the 9'-methyl group of aba is oxidized. analogs of (+)-aba deuterated at the 8'-carbon atom and at both the 8'- and 9'-carbon atoms were fed to green siliques, and extracts containing the deuterated oxidized metabolites were analyzed to determine ... | 2004 | 14671016 |
| a simple method for in vivo expression studies of xanthomonas axonopodis pv. citri. | a major problem in studying bacterial plant pathogens is obtaining the microorganism directly from the plant tissue to perform in vivo expression (protein or mrna) analyses. here we report an easy and fast protocol to isolate xanthomonas axonopodis pv. citri directly from the host plant, in sufficient amounts to perform protein fingerprinting by 2-d gel electrophoresis as well as rna expression assays. the protein profile obtained was very similar to that of x. axonopodis pv. citri grown in the ... | 2003 | 14669917 |
| polymorphism of a specific region in gene p23 of citrus tristeza virus allows discrimination between mild and severe isolates. | the pathogenicity determinants of citrus tristeza virus (ctv) are presently unknown, although transgenic mexican limes over-expressing ctv p23, an rna-binding protein involved in regulating the asymmetrical accumulation of viral rna strands, display typical ctv symptoms. here we compared the predominant sequence variants of gene p23 from 18 ctv isolates of different geographic origin and pathogenicity characteristics. phylogenetic analysis of these sequences revealed three groups of isolates: i) ... | 2003 | 14648289 |
| genetically modified crops: environmental and human health concerns. | about 10,000 years ago subsistence farmers started to domesticate plants and it was only much later, after the discovery of the fundaments of genetics, those organisms were submitted to rational genetic improvement mainly by selecting of traits of interest. breeders used appropriate gene combinations to produce new animal races, plant varieties and hybrids, as well as improved microorganisms such as yeasts. after the introduction of recombinant dna techniques, the transfer of dna between species ... | 2003 | 14644324 |
| expressed sequence enrichment for candidate gene analysis of citrus tristeza virus resistance. | several studies have reported markers linked to a putative resistance gene from poncirus trifoliata ( ctv-r) located at linkage group 4 that confers resistance against one of the most important citrus pathogens, citrus tristeza virus (ctv). to be successful in both marker-assisted selection and transformation experiments, its accurate mapping is needed. several factors may affect its localization, among them two are considered here: the definition of resistance and the genetic background of prog ... | 2004 | 14624336 |
| qtl analysis of citrus tristeza virus-citradia interaction. | citrus tristeza virus (ctv) has caused the death of millions of trees grafted on sour orange ( citrus aurantium). however, this rootstock is very well adapted to the mediterranean, semi-arid conditions. the aim of the present research is to genetically analyze the accumulation of ctv in a progeny derived from the cross between c. aurantium and poncirus trifoliata, both resistant to ctv isolate t-346. graft propagation of 104 hybrids was done on healthy sweet orange as a rootstock. three months l ... | 2004 | 14614564 |
| factors influencing agrobacterium-mediated embryogenic callus transformation of valencia sweet orange (citrus sinensis) containing the pta29-barnase gene. | valencia sweet orange (citrus sinensis (l.) osbeck) calluses were used as explants to develop a new transformation system for citrus mediated by agrobacterium tumefaciens. factors affecting agrobacterium-mediated transformation efficiency included mode of pre-cultivation, temperature of cocultivation and presence of acetosyringone (as). the highest transformation efficiency was obtained with a 4-day pre-cultivation period in liquid medium. transformation efficiency was higher when cocultivation ... | 2003 | 14597430 |
| effect of benzyladenine on recovery of cryopreserved shoot tips of grapevine and citrus cultured in vitro. | the effect of n6-benzyladenine (ba) on the recovery of cryopreserved shoot tips of the ln33 hybrid (vitis l.) and troyer citrange [poncirus trifoliata (l.) raf. x citrus sinensis [l.] osbeck.] cultured in vitro was examined. for the ln33 hybrid, the presence of ba in the recovery medium was essential for survival of control and cryopreserved shoot tips, although the ba concentration did not influence the survival percentage. ba at 5, 2, and 5 microm or higher induced callus formation in control, ... | 2016 | 14566389 |
| desiccation and freezing tolerance of embryonic axes from citrus sinensis [l.] osb. pretreated with sucrose. | embryonic axes of citrus sinensis l. were successfully cryopreserved. while fully hydrated unfrozen axes germinated 100%, survival decreased as axes water content dropped, and total loss of viability was observed when the water content dropped to 0.04 and 0.10 mg h2o/mg dry mass, for axes without and with sucrose preculture, respectively. fully hydrated axes did not survive exposure to liquid nitrogen. highest seedling recovery (93-100%) for untreated axes was observed at 0.26 to 0.15 mg h2o/mg ... | 2009 | 14566388 |
| in vitro hypoglycemic effects of different insoluble fiber-rich fractions prepared from the peel of citrus sinensis l. cv. liucheng. | insoluble fiber-rich fractions (frfs), including insoluble dietary fiber, alcohol-insoluble solid, and water-insoluble solid, were isolated from the peel of citrus sinensis l. cv. liucheng. we found that these three frfs could effectively adsorb glucose, retard glucose diffusion, and inhibit the activity of alpha-amylase to different extents. these mechanisms might create a concerted benefit in decreasing the rate of glucose absorption and eventually lower the concentration of postprandial serum ... | 2003 | 14558787 |
| analysis of gene expression in two growth states of xylella fastidiosa and its relationship with pathogenicity. | xylella fastidiosa is a plant pathogen responsible for diseases of economically important crops. although there is considerable disagreement about its mechanism of pathogenicity, blockage of the vessels is one of the most accepted hypotheses. loss of virulence by this bacterium was observed after serial passages in axenic culture. to confirm the loss of pathogenicity of x. fastidiosa, the causing agent of citrus variegated chlorosis (cvc), freshly-isolated bacteria (first passage [fp] condition) ... | 2003 | 14558688 |
| fruit load and canopy shading affect leaf characteristics and net gas exchange of 'spring' navel orange trees. | five-year-old 'spring' navel (citrus sinensis (l.) osbeck) orange trees were completely defruited, 50% defruited or left fully laden to study effects of fruit load on concentrations of nitrogen (n) and carbohydrate, net assimilation of co2 (ac) and stomatal conductance (gs) of mature leaves on clear winter days just before fruit harvest. leaves on defruited trees were larger, had higher starch concentrations and greater leaf dry mass per area (ldma) than leaves on fruited trees. both ac and gs w ... | 2003 | 14532013 |
| improvement in growth and salt resistance of lemon (citrus limon) trees by an interstock-induced mechanism. | interstocks can reduce toxic ion accumulations in leaves of budded citrus trees, but the mechanism is not understood. we grew sour orange (citrus aurantium l.; so) seedlings, budded trees of 'salustiano' orange (citrus sinensis l. osbeck; sao) on so, 'verna' lemon (citrus limon l. burm. f; vl/so) and interstock trees (vl/sao/so) in pots of sand watered with nutrient solution containing 5 (control) or 50 mm nacl (saline treatment) for 12 weeks. plants were harvested in six successive harvests and ... | 2003 | 14532011 |
| native and introduced host plants of anastrepha fraterculus and ceratitis capitata (diptera: tephritidae) in northwestern argentina. | wild or commercially grown, native and exotic fruit were collected in 30 localities in the tucumán province (nw argentina) from january 1990 to december 1995 to determine their status as hosts of anastrepha fraterculus (wiedemann) and/or ceratitis capitata (wiedemann), the only two fruit fly species of economic and quarantine importance in argentina. a total of 84,094 fruit (3,466.1 kg) representing 33 species (7 native and 26 exotic) in 15 plant families were sampled. we determined the followin ... | 2003 | 14503581 |
| isolation and identification of alcohols in cold-pressed valencia orange oil by liquid-liquid extraction and gas chromatography. | 1965 | 14274120 | |
| purification and identification of flavanone glycosides in the peel of the sweet orange. | 1960 | 13818662 | |
| oil of sweet orange: a possible role in carcinogenesis. | 1959 | 13662498 | |
| leaf volatile compounds of seven citrus somatic tetraploid hybrids sharing willow leaf mandarin (citrus deliciosa ten.) as their common parent. | volatile compounds were extracted by a pentane/ether (1:1) mixture from the leaves of seven citrus somatic tetraploid hybrids sharing mandarin as their common parent and having lime, eurêka lemon, lac lemon, sweet orange, grapefruit, kumquat, or poncirus as the other parent. extracts were examined by gc-ms and compared with those of their respective parents. all hybrids were like their mandarin parent, and unlike their nonmandarin parents, in being unable to synthesize monoterpene aldehydes and ... | 2003 | 13129309 |
| [first supplement to the bibliography on citrus sinensis]. | 2009 | 13125380 | |
| [recurrent dermatitis of the hands caused by contact with oranges; intolerance to sweet orange juice]. | 1953 | 13118905 | |
| [effects of high temperature stress on net photosynthetic rate and photosystem ii activity in citrus]. | table adc-225-mk3 co2 gas analyzer and pam-2000 portable fluorescence measurement system were used to measure the net photosynthetic rate (pn), initial fluorescence (fo), maximal photochemical efficiency of ps ii (fv/fm) and electron transport rate (etr) of satsuma mandarin (citrus unshiu marc.) and navel orange (c. sinensis osbeck) leaves. the results showed that exposure of citrus plants to high temperature (38-40 degrees c) led to a lowering of pn, fv/fm, and etr, whereas fo increased. after ... | 2003 | 12973985 |
| sodium sulphite yields improved dna of higher stability for pcr detection of citrus yellow mosaic virus from citrus leaves. | citrus yellow mosaic virus (cymv), a non-enveloped bacilliform dna virus causes a severe mosaic disease in sweet oranges in india. cymv is weakly immunogenic, thus serodiagnosis is not a preferred method for its detection. as an alternative a rapid and reliable detection protocol by polymerase chain reaction (pcr) was developed. however, high levels of polyphenolics and tannins in citrus leaves generally interfered with obtaining good quality dna, and thus affected the reliable detection of viru ... | 2003 | 12951224 |
| chiral separation of diastereomeric flavanone-7-o-glycosides in citrus by capillary electrophoresis. | the 2s- and 2r-diastereomers of major flavanone-7-o-glycosides found in sweet orange (citrus sinensis), mandarine (citrus deliciosa), grapefruit (citrus paradisi), lemon (citrus limon), and sour or bitter orange juice (citrus aurantium) were separated for the first time by chiral capillary electrophoresis (ce) employing various buffers with combined chiral selectors. native cyclodextrins (cds), neutral and charged cd derivatives were examined as chiral additives to the background electrolyte (bg ... | 2003 | 12900888 |
| the use of the pmi/mannose selection system to recover transgenic sweet orange plants (citrus sinensis l. osbeck). | a new method for obtaining transgenic sweet orange plants was developed in which positive selection (positech) based on the escherichia coli phosphomannose-isomerase (pmi) gene as the selectable marker gene and mannose as the selective agent was used. epicotyl segments from in vitro-germinated plants of valencia, hamlin, natal and pera sweet oranges were inoculated with agrobacterium tumefaciens eha101-pnov2116 and subsequently selected on medium supplemented with different concentrations of man ... | 2003 | 12879258 |
| the compositional characterisation and antioxidant activity of fresh juices from sicilian sweet orange (citrus sinensis l. osbeck) varieties. | epidemiological evidence has suggested that consumption of fruit and vegetables reduces the risk of both cancer and cardiovascular diseases, potentially through the biological actions of components such as vitamin c, vitamin e, flavonoids and carotenoids. citrus species are extremely rich sources in vitamin c and flavanones, a class of compounds which belongs to the flavonoids family. a comparison of the phenolic compositions, the ascorbic acid contents and the antioxidant activities of fresh si ... | 2003 | 12868495 |
| molecular characterization of cytoplasmic and nuclear genomes in phenotypically abnormal valencia orange (citrus sinensis) + meiwa kumquat (fortunella crassifolia) intergeneric somatic hybrids. | organelle dna inheritance of four 10-year-old somatic hybrid trees between valencia orange [citrus sinensis (l.) osbeck] and meiwa kumquat (fortunella crassifolia swingle) was analyzed by cleaved amplified polymorphic sequence (caps) and restriction fragment length polymorphisms (rflps). five chloroplast (cp) and three mitochondrial (mt) universal primer pairs were amplified, but no polymorphisms were detected. when the polymerase chain reaction products were digested by 15 restriction enzymes, ... | 2003 | 12789447 |
| volatile emissions of navel oranges as predictors of freeze damage. | volatile emissions of navel orange (citrus sinensis l. osbeck cv. washington) fruit were evaluated as a means for predicting and gauging freeze damage. the fruits were subjected to -5 or -7 degrees c treatments in a laboratory freezer for various time periods of 2-9.5 h and stored at 23 degrees c for 1, 2, or 7 days, after which time the emission of volatiles from the fruit was measured. following the final day of volatile measurements the fruits were stored at 5 degrees c for an additional 2-3 ... | 2003 | 12744668 |
| comparison of the chemical composition and physicochemical properties of different fibers prepared from the peel of citrus sinensis l. cv. liucheng. | fiber-rich fractions (frfs) including soluble and insoluble dietary fibers (sdf and idf), alcohol-insoluble solid (ais), and water-insoluble solid (wis) were isolated from the peel of citrus sinensis l. cv. liucheng for analysis and tests. the peel was rich in insoluble frfs (idf, ais, and wis; 476-515 g kg(-1) of peel), which were mainly composed of pectic substances and cellulose, and also contained pectic polysaccharide-rich sdf (94.1 g kg(-1) of peel). these insoluble frfs had water-holding ... | 2003 | 12696946 |
| biochemical characterization of blood orange, sweet orange, lemon, bergamot and bitter orange. | this paper reports on the composition of aroma compounds and fatty acids and some physico-chemical parameters (juice percentage, acidity and total sugars) in five varieties of citrus: blood orange, sweet orange, lemon, bergamot and bitter orange. volatile compounds and methyl esters have been analyzed by gas chromatography. limonene is the most abundant compound of monoterpene hydrocarbons for all of the examined juices. eighteen fatty acids have been identified in the studied citrus juices, the ... | 2003 | 12648552 |
| separation and characterization of a salt-dependent pectin methylesterase from citrus sinensis var. valencia fruit tissue. | a pectin methylesterase (pme) from sweet orange fruit rag tissue, which does not destabilize citrus juice cloud, has been characterized. it is a salt-dependent pme (type ii) and exhibits optimal activity between 0.1 and 0.2 m nacl at ph 7.5. the ph optimum shifted to a more alkaline range as the salt molarity decreased (ph 8.5-9.5 at 50 mm nacl). it has an apparent molecular mass of 32.4 kda as determined by gel filtration chromatography, an apparent molecular mass of 33.5 kda as determined by d ... | 2003 | 12643675 |
| juice components of a new pigmented citrus hybrid citrus sinensis (l.) osbeck x citrus clementina hort. ex tan. | fruit juice of a new pigmented citrus hybrid named omo-31 and those of its parents clementine cv. oroval (citrus clementina hort. ex tan.) and moro orange [citrus sinensis (l.) osbeck] were analyzed during fruit maturation to determine juice yield, total soluble solids (tss), total acidity (ta), tss/ta ratio (classical parameters of quality), and potential health beneficial components, such as vitamin c, flavanones, anthocyanins, and phenolic acids. results showed that juice yield, ta, tss, and ... | 2003 | 12617593 |
| simultaneous determination of cd(ii), cu(ii), pb(ii), and zn(ii) in citrus essential oils by derivative potentiometric stripping analysis. | citrus essential oils are widely used in the food, cosmetics, and pharmaceutical industries, so the determination of heavy metals content is of great importance to guarantee their quality. the present work deals with the quantification of cd(ii), cu(ii), pb(ii), and zn(ii) in different varieties of citrus essential oils, using derivative potentiometric stripping analysis. two different metals extraction procedures, involving concentrated hydrochloric acid treatment and acid-alcoholic dissolution ... | 2003 | 12590445 |
| chloride absorption in salt-sensitive carrizo citrange and salt-tolerant cleopatra mandarin citrus rootstocks is linked to water use. | in this work, seedlings of two citrus rootstocks, the salt-tolerant cleopatra mandarin (citrus reshni hort. ex tan.) and the salt-sensitive carrizo citrange (citrus sinensis [l.] osb. x poncirus trifoliata [l.] raf.) were used to study the relationship between chloride and water uptake. the results indicated that net chloride uptake rates in both genotypes were alike and decreased linearly with the time of salinity exposure, although they were more rapidly reduced in the tolerant genotype. in ea ... | 2003 | 12554725 |
| characterization of pinalate, a novel citrus sinensis mutant with a fruit-specific alteration that results in yellow pigmentation and decreased aba content. | the characterization of a novel mutant, named pinalate, derived from the orange (citrus sinensis l. osbeck) navelate, which produces distinctive yellow fruits instead of the typical bright orange colouration, is reported. the carotenoid content and composition, and aba content in leaf and flavedo tissue (coloured part of the skin) of fruits at different developmental and maturation stages were analysed. no important differences in leaf carotenoid pattern of both phenotypes were found. however, a ... | 2003 | 12554716 |
| [molecular cloning of full length capsanthin/capsorubin synthase homologous gene from orange (citrus sinensis)]. | the complete sequence of orange homologous capsanthin/capsorubin synthase gene is 3788 bp long with a coding sequence of 1512 bp, which encodes a polypeptide of 503 amino acids. the 5' upstream sequence is 1721 bp long and the 3' downstream sequence is 555 bp long. the amino acid sequence of this gene is 78% and 69% identical to the genes from carrot and pepper, respectively. it is also partially homologous to plant neoxanthin synthase, lycopene beta-cyclase and lycopene epsilon cyclase genes. i ... | 2001 | 12549109 |
| [research on the separation of limonoid glucosides by reversed-phase preparative high performance liquid chromatography]. | obacunone-17-beta-d-glucopyranoside (og) was isolated from the seeds of citrus sinensis osbeck by using solvent extraction, classical polymer adsorption column separation and weak base anion ion-exchange separation, og was finally purified by c18 reversed-phase preparative high performance liquid chromatography and was identified by thin-layer chromatography. the purity of og was analyzed by analytical reversed-phase hplc. at last the structure of og was determined by 1h and 13c nuclear magnetic ... | 2000 | 12541582 |
| vitamin c, provitamin a carotenoids, and other carotenoids in high-pressurized orange juice during refrigerated storage. | vitamin c, provitamin a carotenoids, and other carotenoids were measured in freshly squeezed juices from oranges (citrus sinensis l. var. valencia late) that were subjected to high-pressure (hp) treatment. also, the stability of these compounds was studied during refrigerated storage at 4 degrees c. hp treatment is an alternative to heat preservation methods for foods; therefore, it is essential to assess the impact of hp on bioactive compounds. several processes that combine hp treatment with h ... | 2003 | 12537436 |
| moderate shade can increase net gas exchange and reduce photoinhibition in citrus leaves. | daily variations in net gas exchange, chlorophyll a fluorescence and water relations of mature, sun-acclimated grapefruit (citrus paradisi macfady.) and orange (citrus sinensis l. osbeck) leaves were determined in tree canopies either shaded with 50% shade screens or left unshaded (sunlit). mean daily maximum photosynthetic photon flux density (ppfd) under shade varied from 500 to 700 micromol m-2 s-1 and was sufficient to achieve maximum net co2 assimilation rates (a co2). responses of grapefru ... | 2003 | 12533306 |