Publications

[the presence of genital mycoplasmas in women of reproductive age].	the authors examined 70 original samples obtained from 70 women seen at the nuevo veranillo community health center because of suspected mycoplasma infection. 23 (33%) of the cultures were positive for mycoplasma. the microscopic and chemical characteristics identified 3 (13%) as ureaplasma urealyticum, 9 (39%) were mycoplasma hominis and 11 (48%) were positive for both organisms. the rate of positive cultures for mycoplasma was high, which could be an important finding since these organisms are ...	1993	8146353
bacterial vaginosis and vaginal microorganisms in idiopathic premature labor and association with pregnancy outcome.	the vaginal microflora of 49 women in idiopathic preterm labor was compared with that of 38 term controls to determine whether the presence of bacterial vaginosis (bv) and/or specific microorganisms would influence the rate of preterm delivery. demographic factors, pregnancy outcome, and reproductive history were also studied. bv, as defined by the presence of clue cells in a vaginal wet mount and characteristic microbial findings in a stained vaginal smear and vaginal culture, was more common i ...	1994	8126176
expectant management of premature rupture of membranes with amniotic fluid cultures positive for ureaplasma urealyticum alone.	our purpose was to determine maternal and neonatal outcome in patients with premature rupture of membranes and amniotic fluid cultures positive for ureaplasma urealyticum alone.	1994	8116718
analysis of a mycoplasma hominis membrane protein, p120.	the monoclonal antibody mab 26.7d generated against a clinical isolate of mycoplasma hominis 7488 was shown to react with a surface-exposed epitope on a 120-kda protein (p120). the gene encoding the protein was cloned and sequenced, and the transcriptional start point was determined by primer extension analysis. the gene contained an open reading frame of 3237 bp encoding a peptide of 1079 amino acids with a deduced molecular mass of 123 kda. a putative amino-terminal signal peptide and cleavage ...	1994	8082822
changes in vaginal flora during pregnancy and association with preterm birth.	a prospective study of the vaginal flora changes between midtrimester and labor was undertaken in 560 women; 337 had intact membranes and were in labor when swabs were taken. of these, 45 delivered preterm. gardnerella vaginalis, ureaplasma urealyticum (both associated with preterm birth when present in midtrimester), and mycoplasma hominis commonly persisted between midtrimester and labor, particularly in the preterm cohort (24 [65%] of 37 midtrimester isolates), and these organisms were seldom ...	1994	8077737
experimentally induced septic arthritis in chimpanzees infected with mycoplasma hominis, mycoplasma pneumoniae, and ureaplasma urealyticum.	mycoplasma hominis was isolated in pure culture from septic synovial aspirates from an individual (patient a) during 16 different bouts of exacerbation over a 70-month period of observation. two isolates, 10(7) and also 10(6) color-changing units (ccu) of the 1620 isolate and 5 x 10(4) ccu of the 1628 isolate, caused inflammation in chimpanzees inoculated intraarticularly. inflammation was also induced with 10(7) ccu of the 2010b isolate, serovar vii of ureaplasma urealyticum, recovered from an ...	1994	8075258
[mycoplasma hominis and ureaplasma urealyticum in pregnant and infertile women. differences in tubal pathology].	we studied the frequency of cervicovaginal bacteria in 47 pregnant women (16-41 weeks of gestational age), in 47 infertile patients and in 4 with gynecological diseases (not infections). when we compared the frequency of bacteria between pregnant and infertile women, we found that the biggest difference was with u. urealyticum. in fact the frequency of this germ was 61% in the total population studied, in pregnant women was of 72% which is statistically higher than the frequency in infertile wom ...	1993	7991855
bacterial vaginosis-associated microflora and effects of topical intravaginal clindamycin.	the goals of this study were as follows: (1) to further define the microbiologic characteristics of bacterial vaginosis in nonpregnant women, (2) to evaluate the in vivo activity of topical intravaginal clindamycin and reference this activity to clinical cure, and (3) to evaluate for clindamycin-related emergence of species.	1994	7977519
prevalence of genital chlamydia and mycoplasma infection in couples attending a male infertility clinic.	to investigate the prevalence of genital mycoplasma (ureaplasma urealyticum and mycoplasma hominis) and chlamydia trachomatis infections in infertile and fertile couples, 135 infertile couples from a male infertility clinic and 88 fertile couples from a prenatal care clinic were randomly screened. semen, urethral and cervical smears were cultured for genital mycoplasma and chlamydia. antichlamydia iga, igg and igm antibodies were tested in blood and semen. although the overall prevalence of urea ...	1994	7957794
detection of chlamydia trachomatis antigen & toxoplasma gondii (igm) & mycoplasma hominis (igg) antibodies by elisa in women with bad obstetric history.	a total of 365 non pregnant women with bad obstetrics history (boh) were studied with a control group of 100 women who had delivered full term clinically normal infants. the presence of c. trachomatis antigen and t. gondii (igm) and m. hominis (igg) antibodies was assessed by elisa test. c. trachomatis antigen was detected in 28.2 per cent of women with boh whereas t. gondii and m. hominis specific antibodies were found in 43.83 and 27.1 per cent respectively, these were highly significant (p < ...	1994	7927547
[investigations on the present status of infections with chlamydia trachomatis (ct), mycoplasma hominis (mh) and ureaplasma urealyticum (uu) in patients with venereal diseases, sexual abusers and healthy people in da lian].	from january to june 1992, we conducted the detection of serum antibodies against ct, mh and uu in patients with venereal diseases, sexual abusers and healthy people. the results showed that the positive rates of antibodies against ct, mh and uu in patients were the highest, while in healthy people those of antibodies against ct and uu were the lowest. the positive rates between the antibody against uu and that against mh have no obvious difference between male and female. all samples have dual ...	1994	7923339
antibodies to ureaplasma urealyticum in women with intraamniotic infection and adverse pregnancy outcome.	to determine the association between antibody response to ureaplasma urealyticum and pregnancy outcome in women with ureaplasmal invasion of the amniotic cavity.	1995	7900509
mycoplasma hominis septic arthritis: two case reports and review.	mycoplasma hominis is normally a commensal of humans. when the organism is pathogenic, it primarily causes disease in the genitourinary tract. septic arthritis caused by m. hominis is a rare condition that occurs chiefly in the postpartum period, in immunosuppressed hosts, or in patients who have recently undergone urinary tract manipulation. arthritis caused by m. hominis is clinically indistinguishable from septic arthritis caused by other bacteria. diagnosis is often delayed because infection ...	1994	7888535
in vitro antimycoplasmal activities of rufloxacin and its metabolite mf 922.	the in vitro activities of rufloxacin and its metabolite, mf 922, were compared with those of ofloxacin, ciprofloxacin, erythromycin, and minocycline against mycoplasma pneumoniae, mycoplasma hominis, mycoplasma fermentans, and ureaplasma urealyticum. rufloxacin, mf 922, and ciprofloxacin shared similar activities against all mycoplasmas tested. (mics for 90% of isolates tested [mic90s], 0.5 to 4 micrograms/ml. ofloxacin had the lowest mic90s for u. urealyticum, m. fermentans, and m. hominis (mi ...	1994	7872762
susceptibilities of mycoplasma hominis, mycoplasma pneumoniae, and ureaplasma urealyticum to new glycylcyclines in comparison with those to older tetracyclines.	the glycylcyclines are new tetracycline derivatives that include the n,n-dimethylglycylamido derivative of minocycline (dmg-mino) and the n,n-dimethylglycylamido derivative of 6-demethyl-6-deoxytetracycline (dmg-dmdot). the susceptibilities of mycoplasma pneumoniae, mycoplasma hominis, and ureaplasma urealyticum to dmg-mino, dmg-dmdot, tetracycline, doxycycline, and minocycline were determined by the agar dilution method. the glycylcyclines with mics at which 50% of the isolates are inhibited of ...	1994	7872759
[epidemiological study on the infections of mycoplasma and chlamydia in immoral persons and healthy controls in seven areas of china].	1,284 immoral persons and 1,161 healthy controls, from seven areas of china, were detected for the antibodies to ureaplasma urealyticum (uu), mycoplasma hominis (mh), and chlamydia trachomatis (ct) by indirect hemoagglutination (iha). the results showed that: (1) in the immoral persons, the positive rates of antibody was the highest in uu (25.47%), higher in ct (18.22%), and lower in mh (8.80%); (2) the levels of antibody to mh, uu, and ct were significantly higher in the immoral persons than in ...	1994	7859257
agammaglobulinaemia.	agammaglobulinaemia is the most common of the primary immunodeficiencies. three major types can be distinguished: x-linked agammaglobulinaemia, early-onset agammaglobulinaemia and late-onset agammaglobulinaemia. in x-linked agammaglobulinaemia, the molecular defect has been elucidated, and genetic counseling, prenatal diagnosis and carrier detection have become important issues. the pathogenesis of early- and late-onset agammaglobulinaemia is heterogeneous and usually not within the b-cell linea ...	1994	7838240
[detection of mycoplasma genitalium from male patients with non-gonococcal urethritis by polymerase chain reaction].	mycoplasma genitalium causes urethritis in non-human primates, but studies on its pathogenicity in man have been hampered by the difficulty in isolating this oragnism in culture. we have used a specific polymerase chain reaction to examine the role of m. genitalium in non-gonococcal urethritis (ngu). oligonucleotide primers were used to amplify a 281 bp of 140-kda adhesin gene of m. genitalium. a characteristic pcr product was amplified, when m. genitalium dna was template for the pcr. no amplif ...	1994	7829906
a longitudinal study of bacterial vaginosis during pregnancy.	to determine the longitudinal changes in the incidence of vaginosis in pregnancy.	1994	7826957
genital flora in pregnancy and its association with intrauterine growth retardation.	a study of risk factors for intrauterine growth retardation (iugr) was conducted among a cohort of 13,914 pregnant women enrolled in the multicenter vaginal infections and prematurity study. from 23 through 26 weeks of gestational age, cultures of specimens from the vagina and cervix were done for group b streptococci, neisseria gonorrhoeae, chlamydia trachomatis, trichomonas vaginalis, candida albicans, gardnerella vaginalis, mycoplasma hominis, ureaplasma urealyticum, and anaerobic gram-negati ...	1994	7814541
mycoplasma hominis in pelvic inflammatory disease.	fifty one cases of pelvic inflammatory disease (pid) and sixty one control subjects were screened for the genital mycoplasmas. cervical swabs, urine & blood were collected from the patients and the control subjects and were processed by standard techniques for isolation and identification of bacteria, fungi and mycoplasmas. the rate of isolation of m. hominis from the pid and controls were 54.90% and 16.39% respectively, and this difference was statistically significant (p. < 0.001). metabolic i ...	1994	7814061
[no detection of mycoplasma in 205 conjunctival swabs].	mycoplasma is known to cause pulmonary and urogenital infections in humans. a pathogenicity for the human eye never has been assessed, although they have been observed in the peripheral eye in a few cases of acute and non-specific conjunctivitis, in patients with reiter's disease and in newborns. due to the absence of cell walls, mycoplasma is only sensitive to a few antibiotics. therefore, we presume that mycoplasma has a role in non-specific and chronic conjunctivitis. it is conceivable that i ...	1994	7812104
[genital microbial flora in a population of high obstetrical risk].	there is increasing evidence showing the association between maternal genital infection, prematurity and neonatal or maternal morbidity. to know the genital flora of women attending a high risk pregnancy service, 73 consecutive pregnant women attending the high risk pregnancy service at the hospital san borja-arriarán from may to august 1992 were included in this study, 75.3% of the vaginal discharges corresponded to fluids of normal appearance and 24.7% were altered. the microorganisms most fre ...	1994	7809430
mycoplasma hominis abscess secondary to respiratory tract infection.		1994	7806885
a 135-kilodalton surface antigen of mycoplasma hominis pg21 contains multiple directly repeated sequences.	a monoclonal antibody was used to characterize a 135-kda surface-located membrane protein (lmp1) generally present in mycoplasma hominis strains. the monoclonal antibody, 552, was applied to identify the corresponding gene in an expression library of m. hominis pg21 dna. the m. hominis pg21 lmp1 gene was sequenced, and its gene product was characterized with the goal of elucidating the structure and function of lmp1. a total of 7,196 bp in the lmp1 region was sequenced. an open reading frame of ...	1995	7806360
prevalence and influence of mycoplasma hominis and ureaplasma urealyticum in 218 african pregnant women and their infants.	to estimate the prevalence of ureaplasma urealyticum (uu) and mycoplasma hominis (mh) in the lower genital tract of pregnant women, their evolution during pregnancy, and the effect of these pathogens on the outcome of pregnancy in equatorial africa.	1994	7805974
site of localization of mycoplasma pulmonis and mycoplasma hominis in the genital tract of female mice demonstrated by culture and scanning and immuno-electron microscopy.	thirty young adult mice, of strain balb/c, treated previously with progesterone, were inoculated intravaginally (10 mice) or directly into the uterus (10 mice) with mycoplasma pulmonis and 10 mice remained uninoculated. ten mice not treated with the hormone were also inoculated intrauterinely with m. pulmonis. the same numbers of mice treated with oestradiol were inoculated in the same ways with m. hominis. vaginal swab specimens were obtained from all mice 7, 14 and 28 days after inoculation an ...	1995	7786763
[mycoplasma hominis bacteremia in a multiple-trauma patient].		1995	7783475
the genital flora of women with intraamniotic infection. vaginal infection and prematurity study group.	the relationship of genital flora assessed at the end of the second trimester of pregnancy and intraamniotic infection diagnosed by clinical signs and symptoms during labor was evaluated. women were enrolled at 23-26 weeks of gestation and followed through delivery in the multi-center vaginal infections and prematurity study (1984-1989). among the cohort of 11,989 followed through delivery, 286 (2.4%) developed intraamniotic infection. the recovery of gardnerella vaginalis (relative risk [rr] = ...	1995	7769281
in-vitro antimycoplasmal activity of flurithromycin.	the in vitro activity of flurithromycin, a 14-membered macrolide drug, was found to be similar to that of erythromycin against 41 strains of mycoplasma spp. and 100 strains of ureaplasma urealyticum. all 28 strains of mycoplasma hominis were uniformly resistant to both macrolides with mics > 256 mg/l, u. urealyticum showed intermediate resistance with mic50s of 0.5 and 1 mg/l for erythromycin and flurithromycin, respectively, whereas the ten strains of mycoplasma pneumonia were susceptible to < ...	1995	7768764
[gardnerella vaginalis infection in heterosexual couples. ultrastructural study of desquamation cells of the stratified epithelium].	bacterial vaginosis is one of the most frequent infections during the woman's reproductive age. lactobacilli normal flora is substituted by relatively elevated gardnerella vaginalis (gv), anaerobic bacteroids, mobiluncus and mycoplasma. the purpose of this study is to perform a morphological analysis of possible mechanisms for adhesion and penetration of gv in the heterosexual couple, in squamous epithelium at vaginal wall, as well as in seminal fluid. ten couples with positive gv culture with t ...	1995	7768469
infertility evaluation in fertile women: a model for assessing the efficacy of infertility testing.	a standard infertility evaluation consists of a semen analysis, hysterosalpingogram, post-coital test, endometrial biopsy and laparoscopy. although these tests are well grounded in clinical experience, information on their ability to discriminate between fertile and infertile couples is limited. in this study, we performed standard infertility tests plus two others--sperm antibodies and cervical culture for mycoplasma hominis and ureaplasma urealyticum--on fertile and infertile couples. women in ...	1994	7714149
[use of a diagnostic prospective algorithm for patients with recurrent miscarriage].	the application of a diagnostic algorithm to couples with recurrent pregnancy loss, was evaluated in this descriptive, prospective, clinical trial, at infertility clinic and assisted reproduction unit, instituto nacional de perinatología (inper), méxico city. fifty couples with primary or secondary recurrent pregnancy loss, were studied. a study protocol was applied to evaluate the following factors of miscarriage: anatomic, endocrine, infectious, genetic and immunologic. the frequency of altere ...	1995	7698682
mycoplasma fermentans--hela cell interactions.	a survey of previous evidence for the intracellular localization of mycoplasmas within nonphagocytic cells indicated that it was insufficient to conclude unequivocally that such localization occurred. illustrations of the seemingly intracellular existence of mycoplasma fermentans in the tissues of patients with aids and other patients rekindled interest in the topic of mycoplasmal entrance into epithelial cells. accordingly, the "incognitus" and pg18 strains of m. fermentans were sought and demo ...	1993	7691197
spermicidal and antiviral properties of cholic acid: contraceptive efficacy of a new vaginal sponge (protectaid) containing sodium cholate.	cholic acid (sodium cholate) exhibits a strong spermicidal and antiviral [anti-human immunodeficiency virus (hiv)-1] activity. the same effects are observed for f-5 gel, the active mixture of a new contraceptive sponge (protectaid), which contains sodium cholate in association with low concentrations (0.5%) of nonoxynol-9 and benzalkonium chloride. both cholic acid and the f-5 gel exert a dose-dependent, in-vitro inhibitory effect (i) on the activity of hiv-1 associated reverse transcriptase in ...	1993	7688380
evaluation of intraspecies genetic variation within the 16s rrna gene of mycoplasma hominis and detection by polymerase chain reaction.	mycoplasma hominis is a heterogeneous species with dna-dna hybridization values ranging from 51 to 100%. we report here the sequencing of the 16s rrna gene of a strain (183) that greatly differs from the type strain (pg21) of this species. comparison of 16s rdna sequences from these two strains showed limited differences, indicating that the two strains belong to the same rrna species complex. using these nucleotide sequence data, we established a rapid method for the detection of m. hominis by ...	1993	7684753
[prospective study of neonatal genital mycoplasma colonization and infection].	genital mycoplasmas have been implicated in different neonatal diseases as pneumonia, sepsis and meningitis. this prospective study was conducted to specify their role in these diseases. population and methods--a pharyngeal or tracheal swab specimen for mycoplasmas culture was obtained from 100 infants admitted consecutively to the neonatal care unit (ncu) during the first 24 hours of life. mycoplasma culture of blood and cerebrospinal fluid was also performed. pharyngeal and/or tracheal specime ...	1995	7663651
mycoplasma hominis orbital abscess.		1995	7661738
[the phenotypic traits of mollicutes as their possible phylogenetic markers].	interaction of cells of mollicutes acholeplasma laidlawii pg 8, a. laidlawii var. granulum 18, mycoplasma hominis pg 21, m. pneumoniae fh, m. fermentans pg 118 and their extracellular products with different carbohydrates, plant lectins of different carbohydrate specificity with glycocalix carbohydrates of the same microorganisms has been studied. basing on this study and data from literature a conclusion is made that such phenotypical characteristics as the ability to form extracellular fructos ...	1995	7655655
infection of the amniotic cavity with ureaplasma urealyticum in the midtrimester of pregnancy.	the purpose of this study was to determine the clinical significance of invasion of the amniotic fluid (af) with ureaplasma urealyticum in the midtrimester of pregnancy. amniotic fluid and cervical swabs obtained from 214 asymptomatic women in the midtrimester of pregnancy (16-20 weeks) were cultured for u urealyticum and mycoplasma hominis. inoculum size was determined by quantitative culture. six of the 214 women (2.8%) had ureaplasma in high titers in the af. the pregnancy outcomes of 129 wom ...	1995	7608879
acute pyelonephritis caused by mycoplasma hominis.	a 59-yr-old male alcoholic with bilateral nephrocalcinosis and upper urinary tract stones presented with fever, acute abdominal signs and ascites. laparotomy revealed the presence of 1.5 litres of ascitic fluid and confirmed right-sided acute pyelonephritis. culture of urine from the renal pelvis obtained during surgery was positive for mycoplasma hominis. initial therapy with cefuroxime failed and doxycycline was later initiated when culture was positive for mycoplasma hominis, with definite cl ...	1995	7603755
mycoplasma hominis pneumonia in aboriginal adults.	mycoplasma hominis (m. hominis) is a well recognized extragenital pathogen. its role as a pathogen of the respiratory tract, however, remains difficult to determine. four cases of pneumonia are presented in which m. hominis was isolated from blood, tracheal aspirates or post-mortem lung tissue. all 4 of these isolates were in young aboriginal adult males. m. hominis is the least fastidious of the human mycoplasmas and grows well on most blood-containing bacteriological media. the recognition and ...	1995	7603754
anti-tumor activity of arginine deiminase from mycoplasma argini and its growth-inhibitory mechanism.	two kinds of arginine deiminase (ad, ec 3.5.3.6) were purified from cell extracts of mycoplasma arginini (a-ad) and mycoplasma hominis (h-ad), and their enzymic properties and anti-tumor activities were compared. the a-ad enzyme strongly inhibited the growth of mouse hepatoma cell line mh134 in vitro, and its concentration required for 50% growth inhibition (ic50) was estimated to be about 10 ng/ml. the ic50 value of h-ad against the same cell line was estimated to be about 100 ng/ml, due to its ...	1995	7591961
sternotomy infection due to mycoplasma hominis and ureaplasma urealyticum.	mycoplasma hominis infections outside the urogenital tract are uncommon. an unusual case of sternal infection caused by both mycoplasma hominis and ureaplasma urealyticum is described. this is the first report found in the literature of mixed infection due to these microorganisms at this site. the outcome was favourable after drainage of the surgical wound and antibiotic therapy with clindamycin, gentamicin and doxycycline.	1995	7588844
maternal assessment in the prediction of intrauterine infection in preterm prelabor amniorrhexis.	the objective of this study was to evaluate maternal temperature, heart rate, leukocyte count and c-reactive protein in the prediction of fetal bacteraemia and positive amniotic fluid cultures in 75 pregnancies complicated by preterm prelabor amniorrhexis. cordocentesis and amniocentesis were performed and fetal blood and amniotic fluid were cultured for aerobic and anaerobic bacteria. amniotic fluid was also cultured for ureaplasma urealyticum and mycoplasma hominis. patients were classified in ...	1995	7576165
selection of mycoplasma hominis pg21 deletion mutants by cultivation in the presence of monoclonal antibody 552.	three mutants of mycoplasma hominis pg21 were isolated and shown to contain alterations in the size of a repeat-containing gene encoding a surface-localized 135-kda antigen designated lmp1. the mutants were isolated by cultivating m. hominis for a 3-month period in the presence of lmp1-specific monoclonal antibody (mab) 552. the epitope for mab 552 was localized at the repeated part of the protein. the gene encoding lmp1 is part of a transcriptional complex that contains 9.5 direct repeats of 47 ...	1995	7543881
sequencing analysis reveals a unique gene organization in the gyrb region of mycoplasma hominis.	the homolog of the gyrb gene, which has been reported to be present in the vicinity of the initiation site of replication in bacteria, was mapped on the mycoplasma hominis genome, and the region was subsequently sequenced. five open reading frames were identified flanking the gyrb gene, one of which showed similarity to that which encodes the lica protein of haemophilus influenzae. the organization of the genes in the region showed no resemblance to that in the corresponding regions of other bac ...	1994	7521872
miocamycin. a review of its antimicrobial activity, pharmacokinetic properties and therapeutic potential.	miocamycin is an orally administered 16-membered macrolide antimicrobial drug. it has a spectrum of in vitro activity similar to that of erythromycin, inhibiting a range of gram-positive and gram-negative organisms, atypical microbes and some anaerobes. importantly, miocamycin demonstrates greater in vitro potency than erythromycin against several pathogens including legionella pneumophila, mycoplasma hominis, and ureaplasma urealyticum. equally noteworthy is its activity against erythromycin-re ...	1993	7506653
association between bacterial vaginosis and preterm delivery of a low-birth-weight infant. the vaginal infections and prematurity study group.	bacterial vaginosis is believed to be a risk factor for preterm delivery. we undertook a study of the association between bacterial vaginosis and the preterm delivery of infants with low birth weight after accounting for other known risk factors.	1995	7491137
male urethritis with and without discharge: a clinical and microbiological study.	the definition of male urethritis in the absence of urethral discharge has not been well established. the sensitivity of urethral swabs and first-catch urine is controversial.	1995	7482108
experimental pelvic inflammatory disease provoked by chlamydia trachomatis and mycoplasma hominis in grivet monkeys.	pelvic inflammatory disease was produced by inoculation with mycoplasma hominis and chlamydia trachomatis directly into the fallopian tubes of grivet monkeys. the effects of various routes of infection were examined. the method of inoculation with the infecting agent influenced the resulting clinical spectrum. in these monkeys significant changes in humoral antibodies occurred with inflammation caused by both m. hominis and c. trachomatis. development of cellular antibodies against m. hominis co ...	1980	7468686
the preparation of transforming dna from mycoplasma hominis strain sprott tetr and quantitative studies of the factors affecting the genetic transformation of mycoplasma salivarium strain s9 tets to tetracycline resistance.	dna extracted by a standard method from mycoplasma hominis sprott, resistant to 100 micrograms tetracycline, permitted the quantitative genetic transformation of tetracycline-sensitive mycoplasma salivarium to resistance. the yield was 1 microgram dna/10(9) cells. this dna enabled determination of the optimum conditions for making m. salivarium competent with cacl2 and for studying some factors affecting transformation. mycoplasma salivarium was transformed to resistance to 10, 20, and 30 microg ...	1980	7459727
placental infection with mycoplasma homonis and ureaplasma urealyticum: clinical correlation.	placentas from a clinical study group of 446 high-risk pregnancies and 108 normal pregnancies were cultured for mycoplasma hominis and ureaplasma urealyticum and examined histologically. results were compared and correlated with the clinical history. the recovery rate of u urealyticum, but not of m hominis, was significantly higher in the clinical study than in the control group. isolation of both mycoplasmas was associated with polymorphonuclear leukocyte infiltration of placental membranes, fe ...	1980	7422192
[mycoplasma-induced urethritis].	abacterial urethritis and colpitis in man and woman is frequently produced by ureaplasma urealyticum and mycoplasma hominis. these agents are transmitted by sexual intercourse and they have to be classified as facultative pathogenic. the clinical value and the therapeutical necessity depend on the clinical picture as well as on an exactly follow up microbiological and immunological diagnosis. as in all sexually transmitted diseases the sex-partner too should be examined and given a corresponding ...	1980	7399916
the killing of ureaplasma urealyticum and mycoplasma hominis by povidone-iodine.		1980	7380768
meningitis in a newborn infant caused by mycoplasma hominis.	when 10 days old an infant born after 34-35 weeks of gestation developed meningitis with pleocytosis and a low glucose concentration in the cerebrospinal fluid. mycoplasma hominis was recovered from the cerebrospinal fluid and treatment with doxycycline was given. the strain was later found to be resistant to tetracycline. after institution of lincomycin, cultures for mycoplasmas were negative. the infant, who during the course of the meningitis had developed a transient increase in intracranial ...	1980	7376869
colony morphology, ultrastructure and morphogenesis in mycoplasma hominis, acholeplasma laidlawii and ureaplasma urealyticum.	colonies of mycoplasma hominis, acholeplasma laidlawii (three strains) and ureaplasma urealyticum were examined by light and electron microscopy and their characteristic morphology, ultrastructure and morphogenesis are described. mycoplasma hominis and a. laidlawii, pg8 and oral strains, developed typical 'fried-egg' colonies which were remarkably heterogeneous in size. the colonies of a. laidlawii strain nctc 10116 were more homogeneous and grew mainly on the surface of the agar showing a fine ...	1980	7373281
preliminary study of the flora in the lower genital tracts of sexually active adolescent females in relation to symptoms and inflammatory response.	the lower genital tracts of 20 sexually active adolescent females were examined for the presence of mycoplasma hominis, ureaplasma urealyticum, corynebacterium genitalium, and corynebacterium pseudogenitalium. fifty percent of the asymptomatic adolescent females and 50% of the symptomatic adolescent females were colonized with m. hominis, which is higher than the percentage reported in adult females. none of the asymptomatic adolescent females and only 10% of the symptomatic adolescent females w ...	1981	7333925
infection of a brain abscess of mycoplasma hominis.	persistent fever in a young man after evacuation of a subdural hematoma caused by a depressed skull fracture made it necessary to carry out a computerized tomographic exam of the head that demonstrated a left frontal lobe brain abscess. mycoplasma hominis was recovered from this abscess as the sole infecting organism. serial computerized tomographic scans showed resolution after aspiration and antibiotic therapy.	1981	7309852
mycoplasma hominis septicemia in a burned infant.		1981	7299550
infection with chlamydia trachomatis, mycoplasma hominis and neisseria gonorrhoeae in patients with acute pelvic inflammatory disease.	of 166 women with acute pelvic inflammatory disease, mycoplasma hominis was isolated from the cervix of 91 (54.8%), chlamydia trachomatis was isolated from 37 (22.3%), and neisseria gonorrhoeae was isolated from nine (5.4%). of the 91 women from whom m. hominis was recovered, 39 developed a significant change in titer of serum antibodies to the organism within one month after admission. of the 85 patients from whose cervix m. hominis was not isolated, 11 displayed a significant change in titer o ...	1981	7292212
urogenital mycoplasma infections of man: a review with observations on a recently discovered mycoplasma.	ureaplasma urealyticum organisms (ureaplasmas), mycoplasma hominis, m. fermentans, m. primatum, m. salivarium and m. pneumoniae have been isolated from the genitourinary tract. the first two of these microorganisms are found most frequently. m. hominis is a cause of some cases of postpartum and postabortal fever, acute pyelonephritis and pelvic inflammatory disease. ureaplasmas have been associated with chorioamnionitis, habitual spontaneous abortion, low birthweight, the urethral syndrome in wo ...	1981	7287395
[mycoplasma hominis as a possible cause of spontaneous abortions and premature labor].		1981	7283069
behavior of mycoplasma hominis in a human diploid cell culture system.	the behavior of mycoplasma hominis in normal human embryonic lung fibroblast (hain-55) cell cultures was investigated. multiplication patterns of cell-associated mycoplasmas and of extracellular mycoplasmas in the hain-55 cultures depended upon the size of the inoculum. this relationship did not vary with the number of days the cells had been cultured, nor with the number of hain-55 cell passages. the maximum mycoplasmal growth was obtained with inoculum sizes of 10(5) to 10(6) colony-forming un ...	1981	7278706
acute salpingitis with chlamydia trachomatis isolated from the fallopian tubes: clinical, cultural, and serologic findings.	chlamydia trachomatis was recovered from the fallopian tubes of ten women with acute salpingitis. the median age of the patients was 19 years. the duration of pelvic pain before consulting a physician ranged from three to 27 days (median, seven days). half of the patients complained of irregular bleeding, and nine reported increased vaginal discharge. one patient had a rectal temperature of greater than 38 c, and one had an erythrocyte sedimentation rate of less than 15 mm/hr. at laparoscopy, mi ...	1981	7256492
the effect of genital mycoplasmas on human fetal growth.	the relation between maternal genital colonization by mycoplasmas and fetal growth was examined in a study of 195 women. swabs were taken from the endocervix on three occasions during pregnancy and once post partum. ureaplasma urealyticum organisms (ureaplasmas) were recovered from 42.7 per cent of caucasian women and from 34.6 per cent of asian women at their first antenatal visit. these isolation rates remained similar throughout pregnancy, although there was a decrease in isolation after deli ...	1981	7248235
ureaplasma urealyticum incriminated in perinatal morbidity and mortality.	perinatal morbidity and mortality are associated with colonization of the chorionic surface of the placenta by ureaplasma urealyticum or mycoplasma hominis or both. these organisms are more strongly associated with unfavourable gestational outcome than group b streptococci. chlamydia trachomatis does not appear to be important in the etiology of reproductive casualties. the mechanisms linking the mycoplasmas to perinatal disorders and death are not clear but merit investigation.	1981	7244646
[serological types of mycoplasma hominis isolated from humans].		1980	7206901
the prevalence of ureaplasma urealyticum and mycoplasma hominis in the cervix and anal canal of women.		1981	7185968
infectious disease and perinatal morbidity.	excess perinatal morbidity and mortality continue to be major problems in developed and developing nations. most perinatal deaths occur in infants born weighing less than 2500 g. large expenditures of time, equipment, and personnel have led to striking reductions in neonatal mortality. however, rates of prematurity have not declined. exploration of proven causes of prematurity and low birth weight suggests a role for infection that has hitherto not received sufficient attention. women with sympt ...	1982	7180023
[mycoplasma hominis and spontaneous abortion].		1982	7168470
comparison of two methods for enumeration of mycoplasmas.	two methods of viable cell counts were evaluated for mycoplasma hominis and ureaplasma urealyticum: color change unit50 and colony-forming unit. the color change unit50 method gave higher estimates of cell numbers; furthermore, the color change unit50 values correlated better with the dna content of the cell pellet and the published genome sizes.	1982	7153345
attempts to detect by physicochemical methods plasmid dna in mycoplasmas of human origin before and after transformation to tetracycline resistance.	physicochemical methods have been used to compare mycoplasma dna capable of the genetic transformation of tetracycline resistance with dna from tetracycline-sensitive mycoplasmas and their transformants. these mycoplasmas were isolated from human patients. the dna extracted from mycoplasma hominis tetr resistant to 100 microgram/ml tetracycline transforms tetracycline resistance to sensitive strains of mycoplasma salivarium tets and mycoplasma hominis tets but not mycoplasma fermentans tets. bul ...	1982	7139411
mycoplasma hominis septicemia.	we report a case of mycoplasma hominis septicemia in a patient with chronic lymphocytic leukemia and prostatic obstruction. signs of sepsis followed urinary catheterization, and m. hominis was recovered repeatedly from blood, urine, and pleural fluid. detection in blood was accomplished by routine subculture from grossly negative blood culture bottles.	1982	7130369
[studies on the aetiology of non-gonococcal urethritis (author's transl)].	tests performed on urethral secretion and morning urine specimen identified the causative micro-organism in 143 of 164 males with non-gonococcal urethritis. in 59 patients (36%) there was an infection with chlamydia trachomatis, in 48 (29.2%) with ureaplasma urealyticum in significantly high bacterial counts (greater than or equal to 10(4) kbu/ml) urethral secretion (greater than or equal to 10(3) kbu/ml first urine sample), and in a further 18 patients (11%) double infections with chlamydia tra ...	1982	7106003
[etiological role of mycoplasma hominis in inflammatory diseases of the urogenital tract in pregnant and nonpregnant women].		1982	7102971
isolation and preliminary characterisation of mycoplasmavirus 20-p.	a mycoplasmavirus, mv 20-p, was isolated from the fastidious, slow-growing human mycoplasma 20-p, previously identified by the growth inhibition test as mycoplasma hominis type 2. its broth culture was ultracentrifuged into 35% saccharose, the pellet was resuspensed in buffer, and the suspension was dropped onto laws of the virus indicator acholeplasma laidlawii bcl-13. the virus was washed off the plaque areas by buffer. mv 20-p appeared in electron microscopy as spherical, enveloped particles ...	1982	7098542
evidence for an association between chlamydia trachomatis and cervical intraepithelial neoplasia.	attempts were made to isolate chlamydia trachomatis, mycoplasma hominis and ureaplasma urealyticum from women with cervical intraepithelial neoplasia (cin), from those with microinvasive or invasive cervical cancer, and from a control group of similar women with other gynaecological problems. c. trachomatis was found in a significantly greater proportion of women with cin (8%) or cervical cancer (18%) than in the control group (1%), whereas m. hominis and u. urealyticum were approximately as com ...	1982	7082604
late spontaneous abortion associated with mycoplasma hominis infection of the fetus.	a 35-year-old gravida 4 was admitted in pregnancy week 25 because of premature rupture of the fetal membranes. two days later an intra-uterine infection developed. treatment with clindamycin and ampicillin was started. however, she aborted within a few hours. the fetus was of normal size and without malformations. mycoplasma hominis could be isolated from the placenta, cerebrospinal fluid and lungs of the fetus during sterile autopsy, and also from the genital tract of the patient, who had a hig ...	1982	7071530
massive pericardial effusion due to mycoplasma hominis in a newborn.		1982	7064954
isolation of mycoplasma hominis from the blood of men with multiple trauma and fever.		1982	7053444
aetiology and management of non-specific vaginitis.	in a study of 100 women with non-specific vaginitis, characterised by a vaginal discharge which was malodorous and pruritic in most cases and caused dyspareunia and dysuria in some, gardnerella vaginalis was isolated in 46% of patients. when present, g vaginalis was significantly associated with mycoplasma hominis and bacteroides species. isolation of g vaginalis was unrelated to the presenting symptoms. treatment with povidone-iodine pessaries for two weeks produced no pronounced benefit, eithe ...	1982	7034858
clinical and microbiological study of non-gonococcal urethritis with particular reference to non-chlamydial disease.	a double-blind placebo-controlled study of minocycline in 221 men with non-gonococcal urethritis (ngu) was undertaken. techniques were used which enabled diagnoses of chlamydial and mycoplasmal infections to be made within 24 hours of a patient attending a clinic. all patients from whom chlamydia trachomatis was isolated were treated with minocycline, while patients from whom ureaplasma urealyticum or mycoplasma hominis was isolated, or from whom no micro-organisms were isolated, were treated on ...	1981	7028208
comparison of serological tests for detection of mycoplasma hominis antibodies in female grivet monkeys with experimentally induced salpingitis.	four serological tests, viz. indirect hemagglutination (iha), metabolism inhibition (mi), immunofluorescence (imf), and a modification of the growth-inhibition (gi) test have been compared for the detection of antibodies against mycoplasma hominis in female grivet monkeys with experimentally induced pelvic inflammatory disease. moreover, cold hemagglutinins (cha), and immunoglobulins, m, g, and a have been determined. the iha test was found to be superior to the other methods used. the antibodie ...	1981	7020337
premature labor. ii. bacterial sources of phospholipase.	human term labor is thought to be initiated by amniotic and chorionic phospholipase a2, an enzyme that liberates arachidonic acid esters from the phospholipids of these membranes, leading to the synthesis of prostaglandins by the placental membranes. the striking association of premature labor with intrauterine infection or contamination, urinary tract infection, and early neonatal sepsis led us to study the microorganisms present in these infections for phospholipase a2 activity. activity was f ...	1981	7017516
treatment of acute salpingitis--with special reference to chlamydia trachomatis.	conservative treatment of acute salpingitis should be the rule, surgery the exception. neisseria gonorrhoeae, chlamydia trachomatis, and mycoplasma hominis, are common etiological agents in pelvic inflammatory disease, particularly in women below the age of 25 years. in women above that age as well as in those with two or more episodes of acute salpingitis, there is a higher proportion of tubal infections caused by aerobic and facultatively anaerobic bacteria. apart from some resistant strains, ...	1982	6958017
three years' experience of sexually transmitted diseases in seville, spain.	at present there are no reliable statistics on the relative prevalences of sexually transmitted diseases (stds) in spain. in a report of the first three years' experience in an std diagnostic centre between 1977 and 1979 a total of 879 patients (534 men adn 345 women) were seen. they mainly consisted of university students and the mean age was 22 years in 1977 and 23 years in the following two years. all the patients were examined for syphilis and all women for gonorrhoea and trichomoniasis. inv ...	1981	6894560
follicular cervicitis--colposcopic appearances and association with chlamydia trachomatis.	follicular cervicitis was recognised in 15 (44 per cent) of 34 women who were examined colposcopically and who were sexual partners of men with non-gonococcal urethritis. valid results of culture for chlamydia trachomatis were obtained in 26 cases: the organism was isolated from the cervix of five of 11 women in whom follicular cervicitis had been diagnosed, but from only one of 15 whose cervices did not have this change. a similar correlation was not found for infection with mycoplasma hominis ...	1981	6893939
cervical chlamydia trachomatis and mycoplasmal infections in pregnancy. epidemiology and outcomes.	in a prospective study of chlamydial and mycoplasmal infections in pregnancy, chlamydia trachomatis occurred in 8.0%, mycoplasma hominis in 23.5%, and ureaplasma urealyticum in 72.3% of 1,365 enrollees. by multivariate analysis, c trachomatis was correlated with lower socioeconomic status, age 23 years or younger, and 12 years or less of schooling. ureaplasma urealyticum was correlated with age 23 years or younger and lower socioeconomic status. mycoplasma hominis was correlated with more than o ...	1983	6887487
septic arthritis due to mycoplasma hominis.		1983	6882481
mycoplasma hominis infection of the central nervous system in a neonate.	a newborn infant with group b streptococcal sepsis responded to the intravenous administration of antibiotics within 3 days, but then began to show signs of central nervous system infection. routine cultures of cerebrospinal fluid samples were negative, but others done for mycoplasma hominis yielded that organism. after 3 more days of antibiotic therapy, cultures for m. hominis were negative, and the child's recovery was uneventful.	1983	6871803
serologic evidence of ureaplasma urealyticum infection in women with spontaneous pregnancy loss.	among 71 couples with histories of pregnancy wastage, 84.5% were colonized with ureaplasma urealyticum and/or mycoplasma hominis; whereas in couples with successful deliveries the incidence was 25.4%. the distribution of u. urealyticum and m. hominis was comparable in the fertile and infertile populations. of women with positive cultures, 96% aborted, compared with an expected rate of 19% to 45%. serologic studies revealed that, at delivery, 42.9% of infants of mothers with pregnancy losses had ...	1983	6849359
septicemia due to mycoplasma hominis.	mycoplasma hominis was recovered from the blood of 7 patients with various underlying disorders. one patient had a legal abortion, another a caesarian section, both followed by septicemia. three patients had genital infections in pregnancy, followed by septicemia and spontaneous abortion. m. hominis was also recovered from cervix and urethra in 2 of these women. high serum antibody levels against m. hominis were demonstrated in 3 women. one female developed septicemia after cancer surgery, one m ...	1983	6844881
studies on the role of ureaplasma urealyticum and mycoplasma hominis in prostatitis.	it has definitely been demonstrated that ureaplasma urealyticum is one etiologic agent of nongonococcal urethritis, a sexually transmitted disease. for this reason it seemed possible that the organisms might cause ascending inflammatory reactions of the prostate. quantitative determinations of ureaplasmas and mycoplasma hominis, together with localization studies, were therefore performed to elucidate the importance of these microorganisms in patients with chronic prostatitis. u. urealyticum was ...	1983	6842018
a controlled study of genital mycoplasmas in amniotic fluid from patients with intra-amniotic infection.	amniotic fluid (af) was collected via a transcervical, intrauterine catheter from 52 patients with clinical intra-amniotic infection (iai) and from 52 matched, uninfected controls. the af was cultured for mycoplasma hominis and ureaplasma urealyticum as well as for aerobic and anaerobic bacteria. eighteen (35%) patients with iai and four (8%) controls had m. hominis in af (p less than 0.001). twenty-six (50%) patients with iai and 26 (50%) control patients had u. urealyticum in af (difference no ...	1983	6842005
association of genital mycoplasmas with exudative vaginitis in a 10 year old: a case of misdiagnosis.	a 10-year-old girl with a 1-year history of lower genitourinary tract symptoms suggestive of bacterial infection but with numerous negative urine cultures was referred to the university of alabama urology clinic after empirical treatment with multiple antibiotics failed to resolve her symptoms. an extensive urologic evaluation revealed no structural or physiologic abnormalities, but an exudative vaginitis was noted and large numbers of ureaplasma urealyticum and mycoplasma hominis were isolated ...	1983	6823429
attachment of mycoplasma hominis and m. orale to human diploid lung fibroblasts.	the process of attachment of mycoplasma hominis and m. orale to hain-55 cells, derived from normal embryonic human lung, was investigated quantitatively. the attachment reached its maximum within about 2-4 hr at 37 degrees c and increased linearly as a function of the number of organisms present in the system. the relative attachment efficiency of m. hominis was approximately 1% under our experimental conditions. trypsin and edta were effective in detaching particles of m. hominis and m. orale f ...	1981	6793814
antibodies to chlamydia trachomatis, mycoplasma hominis, and neisseria gonorrhoeae in sera from patients with acute salpingitis.	paired sera from 60 consecutive patients with acute salpingitis, confirmed by laparoscopy, were examined for serum antibodies to chlamydia trachomatis, mycoplasma hominis, and neisseria gonorrhoeae. by a microimmunofluorescence (mif) test igm or igg antibodies to c trachomatis or both were present in sera from 80% of the patients' by indirect haemagglutination (iha) tests antibodies to m hominis and n gonorrhoeae pilar antigens were present in 40% and 18% respectively. in a control group of 50 p ...	1981	6783261
sexually transmitted conditions among women college students.	we studied 500 unselected young women who consulted a gynecologist in a student health service. most participants were symptom-free and had normal physical examinations. few sexually transmitted infections were encountered. neisseria gonorrhoeae was recovered from two and trichomonas vaginalis was obtained from 14 of 500 women. chlamydia trachomatis was recovered from 20 (4.6%) of 439 participants. genital warts, genital herpes, and molluscum contagiosum, respectively, were noted in seven, four, ...	1981	6779634
the microbiology and therapy of acute pelvic inflammatory disease in hospitalized patients.	we examined microbial isolates from the endocervical and peritoneal cavity of 30 women hospitalized with acute pid. patients were randomly assigned to one of two antibiotic regimens: amoxicillin, 6 gm by mouth every 24 hours, or aqueous penicillin g, 30 million units and gentamicin, 180 to 240 mg intravenously every 24 hours. we measured response by quantifying physical examination findings. neisseria gonorrhoeae was isolated from the cervix of 24 patients (80%) and from the peritoneal cavity of ...	1980	6766274