Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| identification of risk factors associated with disclosure of false positive bovine tuberculosis reactors using the gamma-interferon (ifnγ) assay. | the gamma-interferon assay (ifnγ) is often used as an ancillary diagnostic test alongside the tuberculin skin test in order to detect mycobacterium bovis infected cattle. the performance of the ifnγ test has been evaluated in many countries worldwide and wider usage as a disease surveillance tool is constrained due to the relatively low and inconsistent specificity at a herd and area level. this results in disclosure of a higher proportion of false positive reactors when compared with the skin t ... | 2013 | 24308747 |
| tuberculosis vaccines: hopes and hurdles. | tuberculosis (tb) remains as one of the most serious public health problems worldwide. it is one of the main causes of death in poor and developing countries, especially in sub-saharan africa, where it may be associated with the human immunodeficiency virus (hiv). it has been estimated that one third of the world population is infected by mycobacterium tuberculosis (mtb), and there were about 8.7 million new tb cases, and about 1.4 million yearly deaths due to tb in 2011. dots is the currently u ... | 2013 | 24304353 |
| draft genome sequence of mycobacterium bovis 04-303, a highly virulent strain from argentina. | mycobacterium bovis strain 04-303 was isolated from a wild boar living in a free-ranging field in argentina. this work reports the draft genome sequence of this highly virulent strain and the genomic comparison of its major virulence-related genes with those of m. bovis strain af2122/97 and mycobacterium tuberculosis strain h37rv. | 2013 | 24285661 |
| immunogenicity and protective efficacy conferred by a novel recombinant mycobacterium bovis bacillus calmette-guérin strain expressing interleukin-12p70 of human cytokine and ag85a of mycobacterium tuberculosis fusion protein. | mycobacterium bovis bacillus calmette-guérin (bcg) immunization provides protection against tuberculosis (tb) in infants, but the antituberculosis protective immunity wanes gradually after initial immunization and lasts less than 15 years. therefore, more efficacious vaccines are urgently needed. in this study, we constructed a new tuberculosis vaccine of recombinant bcg strain (rbcg-ia), which could express il-12p70 of human cytokine and ag85a of m. tuberculosis fusion protein, and investigated ... | 2013 | 24283772 |
| enhanced detection of tuberculous mycobacteria in animal tissues using a semi-nested probe-based real-time pcr. | bovine tuberculosis has been tackled for decades by costly eradication programs in most developed countries, involving the laboratory testing of tissue samples from allegedly infected animals for detection of mycobacterium tuberculosis complex (mtc) members, namely mycobacterium bovis. definitive diagnosis is usually achieved by bacteriological culture, which may take up to 6-12 weeks, during which the suspect animal carcass and herd are under sanitary arrest. in this work, a user-friendly dna e ... | 2013 | 24278425 |
| comparison of the immunogenicity and protection against bovine tuberculosis following immunization by bcg-priming and boosting with adenovirus or protein based vaccines. | there is a requirement for vaccines or vaccination strategies that confer better protection against tb than the current live attenuated mycobacterium bovis bacillus calmette-guerin (bcg) vaccine for use in cattle. boosting with recombinant viral vectors expressing mycobacterial proteins, such as ag85a, has shown a degree of promise as a strategy for improving on the protection afforded by bcg. experiments in small animal models have indicated that broadening the immune response to include mycoba ... | 2014 | 24269321 |
| mycobacterium bovis infections in domesticated non-bovine mammalian species. part 1: review of epidemiology and laboratory submissions in great britain 2004-2010. | mycobacterium bovis, the causative agent of bovine tuberculosis (btb), can infect a broad range of mammalian species in addition to domestic and feral cattle and badgers. since legislation introduced in 2006 in great britain requires animal keepers, meat inspectors and veterinarians to notify the authorities of suspect btb lesions or the isolation of m. bovis in any mammal excluding humans, the organism has been increasingly identified in domestic species other than cattle. although in most case ... | 2013 | 24268485 |
| sex-related differences in body condition and serum biochemical parameters in red deer (cervus elaphus) naturally infected with mycobacterium bovis. | although mycobacterium bovis infection is commonly reported in red deer (cervus elaphus), potential differences in the effects of infection on male and female animals in terms of body condition and clinical biochemistry have not been reported. between november 2000 and january 2006, serum and biometrical data were collected post-mortem from 88 red deer. m. bovis-infected deer, particularly males, were typically older, heavier and in poorer body condition than uninfected animals. serum triglyceri ... | 2013 | 24262346 |
| synthesis and antimycobacterial activity of symmetric thiocarbohydrazone derivatives against mycobacterium bovis bcg. | in this work, we reported the synthesis and evaluation of antimycobacterial and antifungal activity of a series of thiocarbohydrazone derivatives which are thiacetazone congeners. the target compounds were synthesized in superior yields by reacting thiocarbohydrazide with different aromatic aldehydes and methyl ketones. compounds 8, 19 and 25 were found to be the most potent derivatives, exhibiting acceptable activity against mycobacterium bovis bcg compared to thiacetazone and ethambutol as ref ... | 2013 | 24250608 |
| identification of the target protein of agelasine d, a marine sponge diterpene alkaloid, as an anti-dormant mycobacterial substance. | one of the major reasons for the wide epidemicity of tuberculosis and for the necessity for extensive chemotherapeutic regimens is that the causative agent, mycobacterium tuberculosis, has an ability to become dormant. therefore, new lead compounds that are anti-bacterial against m. tuberculosis in both active and dormant states are urgently needed. marine sponge diterpene alkaloids, agelasines b, c, and d, from an indonesian marine sponge of the genus agelas were rediscovered as anti-dormant-my ... | 2014 | 24243718 |
| potent antimycobacterial activity of the pyridoxal isonicotinoyl hydrazone analog 2-pyridylcarboxaldehyde isonicotinoyl hydrazone: a lipophilic transport vehicle for isonicotinic acid hydrazide. | the rise in drug-resistant strains of mycobacterium tuberculosis is a major threat to human health and highlights the need for new therapeutic strategies. in this study, we have assessed whether high-affinity iron chelators of the pyridoxal isonicotinoyl hydrazone (pih) class can restrict the growth of clinically significant mycobacteria. screening a library of pih derivatives revealed that one compound, namely, 2-pyridylcarboxaldehyde isonicotinoyl hydrazone (pcih), exhibited nanomolar in vitro ... | 2014 | 24243647 |
| evaluation of single and comparative intradermal tuberculin tests for tuberculosis eradication in caprine flocks in castilla y león (spain). | goats can act as reservoirs for tuberculosis (tb) infection. the main etiological agents of tb in goats are mycobacterium caprae and mycobacterium bovis and they infect also a wide range of domestic and wild animals and humans. control programmes based mainly on the application of single and comparative intradermal tuberculin (sit and scit respectively) tests are being implemented in certain regions of spain with a high density of caprine flocks as castilla y león, including goats with epidemiol ... | 2014 | 24239314 |
| secretory phosphatases deficient mutant of mycobacterium tuberculosis imparts protection at the primary site of infection in guinea pigs. | the failure of mycobacterium bovis bacille calmette-guérin to impart satisfactory protection against adult pulmonary tuberculosis has necessitated the development of more effective tb vaccines. the assumption that the vaccine strain should be antigenically as similar as possible to the disease causing pathogen has led to the evaluation of m.tuberculosis mutants as candidate tuberculosis vaccines. | 2013 | 24205032 |
| juxtaposition between host population structures: implications for disease transmission in a sympatric cervid community. | sympatric populations of phylogenetically related species are often vulnerable to similar communicable diseases. although some host populations may exhibit spatial structure, other hosts within the community may have unstructured populations. thus, individuals from unstructured host populations may act as interspecific vectors among discrete subpopulations of sympatric alternate hosts. we used a cervid-bovine tuberculosis (mycobacterium bovis) system to investigate the landscape-scale potential ... | 2013 | 24187583 |
| sub-speciation of mycobacterium tuberculosis complex from tuberculosis patients in japan. | mycobacterium tuberculosis is the major causative agent of tuberculosis in humans. it is well known that mycobacterium bovis and other species in the m. tuberculosis complex (mtc) can cause respiratory diseases as zoonosis. we analyzed the mtc isolates collected from tuberculosis patients from japan in 2002 using a multiplex pcr system that detected cfp32, rd9 and rd12. a total of 970 mtc isolates that were representative of the tuberculosis cases throughout japan, were examined using this metho ... | 2014 | 24183617 |
| enhancement of antimycobacterial th1-cell responses by a mycobacterium bovis bcg prime-protein boost vaccination strategy. | tuberculosis is a major global health problem, and the only available vaccine bacille calmette-guérin (bcg) is not sufficiently effective against the disease. it is extremely urgent to develop novel vaccine approaches. previous research demonstrated that there were several regions of difference (rd1-16) between the substrains of bcg and mycobacterium tuberculosis or mycobacterium bovis. the orfs rv1769 and rv1772 are located in the rd14 deletions and have not been major targets of study. however ... | 2013 | 24177251 |
| protective immunity induced by a recombinant bcg vaccine encoding the cyclophilin gene of toxoplasma gondii. | the investigation of toxoplasma gondii virulence factors can elucidate the immunopathology of t. gondii infection and identify potential candidates for effective human vaccines. the adjuvant is an important component of an effective vaccine. in this study, attenuated mycobacterium bovis was used as a live vaccine vector with both antigen and adjuvant characteristics. following amplification of the t. gondii cyclophilin gene, the shuttle expression plasmid pmv261-tgcyp and integrative expression ... | 2013 | 24176493 |
| use of antigen-specific interleukin-2 to differentiate between cattle vaccinated with mycobacterium bovis bcg and cattle infected with m. bovis. | we describe here the application of a novel bovine interleukin-2 (il-2) enzyme-linked immunosorbent assay (elisa) for the measurement of antigen-specific il-2 in cattle naturally infected with mycobacterium bovis and in cattle vaccinated with mycobacterium bovis bcg and then experimentally challenged with pathogenic m. bovis. supernatants from whole-blood cultures stimulated with mycobacterial antigen (bovine purified protein derivative [ppdb] or the peptide cocktail esat6-cfp10) were assessed u ... | 2014 | 24173026 |
| assessment of an oral mycobacterium bovis bcg vaccine and an inactivated m. bovis preparation for wild boar in terms of adverse reactions, vaccine strain survival, and uptake by nontarget species. | wildlife vaccination is increasingly being considered as an option for tuberculosis control. we combined data from laboratory trials and an ongoing field trial to assess the risk of an oral mycobacterium bovis bcg vaccine and a prototype heat-inactivated mycobacterium bovis preparation for eurasian wild boar (sus scrofa). we studied adverse reactions, bcg survival, bcg excretion, and bait uptake by nontarget species. no adverse reactions were observed after administration of bcg (n = 27) or inac ... | 2013 | 24173022 |
| overview of vaccination trials for control of tuberculosis in cattle, wildlife and humans. | vaccination is a key strategy for control of tuberculosis (tb), and considerable progress has been made in the past 5 years to develop improved vaccines for humans and animals, differentiate vaccinated animals from those infected with mycobacterium bovis and deliver vaccines to wildlife. studies have moved from testing vaccines in small animal models to clinical trials in humans and from experimental challenge studies in cattle and wildlife to evaluation of vaccines in the field. candidate vacci ... | 2013 | 24171859 |
| control strategies for wildlife tuberculosis in ireland. | the principal domestic maintenance host for mycobacterium bovis is infected cattle. in countries where comprehensive surveillance schemes have been applied, tuberculosis rarely affects an animal to the extent that it presents with clinical disease. in the latter stages of an eradication campaign, the aim is to maintain the disease-free status of clear herds and eliminate foci of infection in herds as well as restricting movement of infected animals from these herds, other than to slaughter. howe ... | 2013 | 24171858 |
| evaluation of the discriminatory power of variable number of tandem repeat typing of mycobacterium bovis isolates from southern africa. | the usefulness of variable number tandem repeat (vntr) typing based on limited numbers of loci has previously proven inferior compared to is6110-rflp typing when applied to the study of the molecular epidemiology of bovine tuberculosis (btb) in both livestock and wildlife in southern africa. in this study, the discriminatory power of 29 published vntr loci in the characterization of 131 mycobacterium bovis strains isolated predominantly from wildlife and a smaller number from livestock in southe ... | 2013 | 24171856 |
| prevalence and distribution of non-tuberculous mycobacteria (ntm) in cattle, african buffaloes (syncerus caffer) and their environments in south africa. | it has been hypothesized that a variety of non-tuberculous mycobacteria (ntm) species to which livestock and wildlife species are naturally exposed induce broadly cross-reactive anti-mycobacterial immune responses which interfere with current standard diagnostic assays. non-tuberculous mycobacteria have also been implicated in mycobacterium bovis-specific immune responsiveness, hence potentially the development of tuberculosis. cattle and african buffaloes are both maintenance hosts of bovine tu ... | 2013 | 24171852 |
| performance of diagnostic tests for bovine tuberculosis in north american furbearers and implications for surveillance. | risks of bovine tuberculosis (btb) transmission from free-ranging wildlife to livestock remain a concern in the united states, in both known endemic areas and where spillover from recently-infected livestock herds occurs. federal agriculture officials in the united states (us) have recommended surveillance of non-cervid furbearers to determine whether free-ranging wildlife in the vicinity of cattle herd breakdowns are btb infected, yet the efficacy of common diagnostic tests in these species is ... | 2013 | 24171851 |
| towards establishing a rhinoceros-specific interferon-gamma (ifn-γ) assay for diagnosis of tuberculosis. | mycobacterium bovis is the causal agent of bovine tuberculosis (btb), with a diverse host range, extending from livestock to domestic and captive wild animals as well as free-ranging wildlife species. in south africa, btb is endemic in the kruger national park (knp) and the hluluwe imfolozi national park (hip), where the high prevalence of m. bovis infections in buffalo herds has led to infection of a number of wildlife species. this has raised concerns about the spillover into the rhinoceros po ... | 2013 | 24171850 |
| sex-related heterogeneity in the life-history correlates of mycobacterium bovis infection in european badgers (meles meles). | heterogeneity in the progression of disease amongst individual wild animals may impact on both pathogen and host dynamics at the population level, through differential effects on transmission, mortality and reproductive output. the role of the european badger (meles meles) as a reservoir host for mycobacterium bovis infection in the uk and ireland has been the focus of intense research for many years. here, we investigate life-history correlates of infection in a high-density undisturbed badger ... | 2013 | 24171847 |
| preliminary assessment of bovine tuberculosis at the livestock/wildlife interface in two protected areas of northern botswana. | protected areas of northern botswana such as the okavango delta (od) or chobe national park (cnp) are well-known hot spots for the conservation of african wildlife. however, their infection status regarding bovine tuberculosis (btb) at the domestic/wildlife interface has never been investigated. to provide preliminary baseline data on the circulation of mycobacterium bovis in those sites, we performed a cross-sectional survey on 130 buffalo in both protected areas (60 individuals from cnp and 70 ... | 2013 | 24171846 |
| mycobacterium bovis: characteristics of wildlife reservoir hosts. | mycobacterium bovis is the cause of tuberculosis in animals and sometimes humans. many developed nations have long-standing programmes to eradicate tuberculosis in livestock, principally cattle. as disease prevalence in cattle decreases these efforts are sometimes impeded by passage of m. bovis from wildlife to cattle. in epidemiological terms, disease can persist in some wildlife species, creating disease reservoirs, if the basic reproduction rate (r0) and critical community size (ccs) threshol ... | 2013 | 24171844 |
| utility of a fecal real-time pcr protocol for detection of mycobacterium bovis infection in african buffalo (syncerus caffer). | a real-time pcr protocol for detecting mycobacterium bovis in feces was evaluated in bovine tuberculosis-infected african buffalo (syncerus caffer). fecal samples spiked with 1.42 × 10(3) cells of m. bovis culture/g and bacille calmette-guérin standards with 1.58 × 10(1) genome copies/well were positive by real-time pcr but all field samples were negative. | 2014 | 24171565 |
| clinical utility of rd1, rd9 and hsp65 based pcr assay for the identification of bcg in vaccinated children. | mycobacterium bovis bacille calmette-guérin (bcg) vaccine is widely administered to prevent tuberculosis. vaccine complications are rare. however, when bcg-related adverse reactions arise there is a need to rapidly and reliably identify bcg from other members of the mycobacterium tuberculosis complex (tbc). pcr assays based on the detection of the regions of difference (rd), in particular rd1 and rd9, have been invaluable in the identification of bcg. prior to this study, specimens were identifi ... | 2013 | 24168785 |
| in vitro anti-mycobacterial activity of selected medicinal plants against mycobacterium tuberculosis and mycobacterium bovis strains. | tuberculosis (tb) is a global burden with one -third of the world's population infected with the pathogen mycobacterium tuberculosis complex and annually 1.4 million deaths occur due to the disease. this high incidence of infection and the increased rate of multi-drug resistant and extensively-drug resistant strains of the organism further complicated the problem of tb control and have called for an urgent need to develop new anti-tb drugs from plants. in this study, the in vitro activity of roo ... | 2013 | 24168665 |
| genotypic characterization by spoligotyping and vntr typing of mycobacterium bovis and mycobacterium caprae isolates from cattle of tunisia. | this work is an approach to the molecular epidemiology of mycobacterium tuberculosis complex (mtbc) bovine infections in tunisia. a total of 35 mtbc isolates from both lateral retropharyngeal lymph node samples of cattle slaughtered in different tunisian regions were genotyped by spoligotyping and variable number tandem repeat typing (vntr)-typing. spoligotyping allowed to identify two profiles not previously registered, namely sb2024, a mycobacterium caprae isolate from nabeul region (north eas ... | 2014 | 24158359 |
| mycobacterium bovis infection: everything but the cow. | 2013 | 24157342 | |
| efficient activation of human t cells of both cd4 and cd8 subsets by urease-deficient recombinant mycobacterium bovis bcg that produced a heat shock protein 70-m. tuberculosis-derived major membrane protein ii fusion protein. | for the purpose of obtaining mycobacterium bovis bacillus calmette-guérin (bcg) capable of activating human naive t cells, urease-deficient bcg expressing a fusion protein composed of mycobacterium tuberculosis-derived major membrane protein ii (mmp-ii) and heat shock protein 70 (hsp70) of bcg (bcg-dhtm) was produced. bcg-dhtm secreted the hsp70-mmp-ii fusion protein and effectively activated human monocyte-derived dendritic cells (dcs) by inducing phenotypic changes and enhanced cytokine produc ... | 2014 | 24152387 |
| isolation and molecular characterization of mycobacterium bovis from kafue lechwe (kobus leche kafuensis) from zambia. | bovine tuberculosis (btb) is a chronic bacterial disease caused by mycobacterium bovis. infections due to m. bovis, which serves as a stable reservoir, can pose serious challenge to control and eradicate in both wildlife and livestock at the interface. this study aimed at isolating and characterizing m. bovis from kafue lechwe (kobus leche kafuensis) and black lechwe (kobus leche smithemani) at the animal/human interface in zambia. the samples with lesions compatible with btb collected during th ... | 2014 | 24146292 |
| immunopathology of granulomas produced by mycobacterium bovis in naturally infected wild boar. | limited information has been published on the wild boar immune response against bovine tuberculosis (btb) and the immunopathogenesis of the pathological hallmark (granuloma) in this species. the main objectives of this study were, on the one hand, to characterize the histopathological features (number of acid-fast bacilli (afb) and multinucleated giant cells (mngcs) and the immunohistochemical distribution of different cell subsets (cd3+, cd79a+ and mac387+) and chemical mediators (inos and ifn- ... | 2013 | 24144683 |
| prokaryotic expression and functional analysis of the mb1514 gene in mycobacterium bovis. | the ability of mycobacteria to grow and invade target tissues is the key component in the process of mycobacterium bovis infection. therefore, analysis of the proteins responsible for cell invasion will assist clinicians in combating bovine tuberculosis. the mb1514 gene of m. bovis encodes a hypothetical invasion protein (designated here as mbinv protein), whose function has not yet been directly identified. in this study, the mb1514 gene from m. bovis was cloned, and expressed in e. coli. the r ... | 2014 | 24141863 |
| mycobacterium bovis in an egyptian mongoose. | 2013 | 24141165 | |
| mycobacterium bovis infections in domesticated non-bovine mammalian species. part 2: a review of diagnostic methods. | despite the large host range of mycobacterium bovis, ante-mortem diagnostic tests for the infection mostly lack sensitivity/specificity and/or remain unvalidated in non-bovine species. the epidemiology and importance of m. bovis infection in these species are discussed in the first part of this two-part review. this second part focuses on the diagnostic options available to identify infected species such as sheep, goats, dogs, cats, and camelids, and highlights the significant challenges posed, ... | 2013 | 24135547 |
| th1-skewed tissue responses to a mycolyl glycolipid in mycobacteria-infected rhesus macaques. | trehalose 6,6'-dimycolate (tdm) is a major glycolipid of the cell wall of mycobacteria with remarkable adjuvant functions. to avoid detection by the host innate immune system, invading mycobacteria down-regulate the expression of tdm by utilizing host-derived glucose as a competitive substrate for their mycolyltransferases; however, this enzymatic reaction results in the concomitant biosynthesis of glucose monomycolate (gmm) which is recognized by the acquired immune system. gmm-specific, cd1-re ... | 2013 | 24134838 |
| genome-level analyses of mycobacterium bovis lineages reveal the role of snps and antisense transcription in differential gene expression. | bovine tuberculosis (btb) is a disease with major implications for animal welfare and productivity, as well as having the potential for zoonotic transmission. in great britain (gb) alone, controlling btb costs in the region of £ 100 million annually, with the current control scheme seemingly unable to stop the inexorable spread of infection. one aspect that may be driving the epidemic is evolution of the causative pathogen, mycobacterium bovis. to understand the underlying genetic changes that m ... | 2013 | 24134787 |
| evaluation of a commercial enzyme-linked immunosorbent assay for the diagnosis of bovine tuberculosis from milk samples from dairy cows. | milk samples from dairy cows provide a ready source of material for measuring antibody responses to mycobacterium bovis antigens. in this study, we evaluated the idexx enzyme-linked immunosorbent assay (elisa) for the measurement of antibody responses to m. bovis antigens mpb70 and mpb83 in milk samples from new zealand cattle. test sensitivities for individual milk and serum samples were assessed in samples collected from 44 m. bovis-infected cows, and test specificities were assessed in milk s ... | 2013 | 24132605 |
| clinical and diagnostic developments of a gamma interferon release assay for use in bovine tuberculosis control programs. | currently, the bovigam assay is used as an official supplemental test within bovine tuberculosis control programs. the objectives of the present study were to evaluate two mycobacterium bovis-specific peptide cocktails and purified protein derivatives (ppds) from two sources, liquid and lyophilized antigen preparations. ppds and peptide cocktails were also used for comparison of a second-generation gamma interferon (ifn-γ) release assay kit with the currently licensed first-generation kit (bovig ... | 2013 | 24132602 |
| development of miliary tuberculosis under infliximab in a patient with spondyloarthritis and suspected crohn's disease. | tuberculosis (tb) infection is a major concern in patients with chronic autoimmune conditions under immunosuppressive therapy. gastrointestinal tuberculosis can be misdiagnosed as crohn's disease with detrimental consequences for the patient. we report on a 40-year old ethnic turkish patient with hla-b27 positive spondyloarthritis who developed gastrointestinal symptoms under immunosuppressive treatment with infliximab. crohn's disease was diagnosed at a primary care hospital and immunosuppressi ... | 2013 | 24122379 |
| differential tlr2 downstream signaling regulates lipid metabolism and cytokine production triggered by mycobacterium bovis bcg infection. | the nuclear receptor pparγ acts as a key modulator of lipid metabolism, inflammation and pathogenesis in bcg-infected macrophages. however, the molecular mechanisms involved in pparγ expression and functions during infection are not completely understood. here, we investigate signaling pathways triggered by tlr2, the involvement of co-receptors and lipid rafts in the mechanism of pparγ expression, lipid body formation and cytokine synthesis in macrophages during bcg infection. bcg induces nf-κb ... | 2014 | 24120921 |
| mycobacterium bovis infection in cattle induces differential expression of prolactin receptor isoforms in macrophages. | prolactin receptor (prlr) is a member of the cytokine receptor superfamily 1 showing tissue specific structural diversity. expression of prlr isoforms in lymphoid tissues has been associated with immunomodulatory function of prolactin. bovine tuberculosis (btb) is characterized by chronic inflammation caused by the persistent infection of lymphoid tissues with mycobacterium bovis. to test the hypothesis of the influence of prlr in the pathogenesis of btb, the aim of this study was to identify pr ... | 2013 | 24119726 |
| a new plant-derived antibacterial is an inhibitor of efflux pumps in staphylococcus aureus. | an in-depth evaluation was undertaken of a new antibacterial natural product (1) recently isolated and characterised from the plant hypericum olympicum l. cf. uniflorum. minimum inhibitory concentrations (mics) were determined for a panel of bacteria, including: meticillin-resistant and -susceptible strains of staphylococcus aureus, staphylococcus epidermidis and staphylococcus haemolyticus; vancomycin-resistant and -susceptible enterococcus faecalis and enterococcus faecium; penicillin-resistan ... | 2013 | 24119569 |
| concurrent granulomatous hepatitis, pneumonitis and sepsis as a complication of intravesical bcg immunotherapy. | intravesical administration of bcg is a relatively simple procedure used successfully in the treatment of superficial transitional cell carcinoma of the urinary bladder. it is usually well tolerated with few major side effects. the authors report the case of an 80-year-old man who presented with sepsis, jaundice, hepatic and pulmonary failure 10 days after his last bcg instillation therapy, that was attributed to concurrent granulomatous hepatitis and pneumonitis due to mycobacterium bovis disse ... | 2013 | 24114600 |
| poly-l-glutamate/glutamine synthesis in the cell wall of mycobacterium bovis is regulated in response to nitrogen availability. | the cell wall of pathogenic mycobacteria is known to possess poly-l-glutamine (plg) layer. plg synthesis has been directly linked to glutamine synthetase (gs) enzyme. glna1 gene encodes for gs enzyme in mycobacteria. plg layer is absent in cell wall of avirulent mycobacterium smegmatis, although m. smegmatis strain expressing gs enzyme of pathogenic mycobacteria can synthesize plg layer in the cell wall. the role of gs enzyme has been extensively studied in mycobacterium tuberculosis, however, l ... | 2013 | 24112767 |
| synthesis and antimycobacterial activity of novel thiadiazolylhydrazones of 1-substituted indole-3-carboxaldehydes. | a series of novel thiocarbohydrazones of substituted indoles and their corresponding thiadiazole derivatives were prepared, and their structures were confirmed by different analytical and spectroscopic methods. the derivatives were prepared by a sequential synthetic strategy including substitution at n-1 position of indole ring by various aliphatic and benzylic substituents, followed by condensation with thiocarbohydrazide, and finally cyclization by triethyl orthoformate. the derivatives were t ... | 2014 | 24103309 |
| systems-based approaches to probing metabolic variation within the mycobacterium tuberculosis complex. | the mycobacterium tuberculosis complex includes bovine and human strains of the tuberculosis bacillus, including mycobacterium tuberculosis, mycobacterium bovis and the mycobacterium bovis bcg vaccine strain. m. bovis has evolved from a m. tuberculosis-like ancestor and is the ancestor of the bcg vaccine. the pathogens demonstrate distinct differences in virulence, host range and metabolism, but the role of metabolic differences in pathogenicity is poorly understood. systems biology approaches h ... | 2013 | 24098743 |
| the contribution of non-conventional t cells and nk cells in the mycobacterial-specific ifnγ response in bacille calmette-guérin (bcg)-immunized infants. | the mycobacterium bovis bacille calmette-guérin (bcg) vaccine is given to >120 million infants each year worldwide. most studies investigating the immune response to bcg have focused on adaptive immunity. however the importance of tcr-gamma/delta (γδ) t cells and nk cells in the mycobacterial-specific immune response is of increasing interest. | 2013 | 24098583 |
| mycobacterium bovis infection in domestic pigs in great britain. | mycobacterium bovis, the causative agent of bovine tuberculosis (tb), infects a wide range of wild and domestic mammals. despite a control programme spanning decades, m. bovis infection levels in cattle in great britain (gb) have continued to rise over recent years. as the incidence of infection in cattle and wildlife may be linked to that in swine, data relating to infection of pigs identified at slaughter were examined in this study. between 2007 and 2011, almost all m. bovis-infected pigs ori ... | 2013 | 24095608 |
| lipoproteins of slow-growing mycobacteria carry three fatty acids and are n-acylated by apolipoprotein n-acyltransferase bcg_2070c. | lipoproteins are virulence factors of mycobacterium tuberculosis. bacterial lipoproteins are modified by the consecutive action of preprolipoprotein diacylglyceryl transferase (lgt), prolipoprotein signal peptidase (lspa) and apolipoprotein n- acyltransferase (lnt) leading to the formation of mature triacylated lipoproteins. lnt homologues are found in gram-negative and high gc-rich gram-positive, but not in low gc-rich gram-positive bacteria, although n-acylation is observed. in fast-growing my ... | 2013 | 24093492 |
| comparison of the membrane proteome of virulent mycobacterium tuberculosis and the attenuated mycobacterium bovis bcg vaccine strain by label-free quantitative proteomics. | the mycobacterium tuberculosis membrane is rich in antigens that are potential targets for diagnostics and the development of new vaccines. to better understand the mechanisms underlying mtb virulence and identify new targets for therapeutic intervention, we investigated the differential composition of membrane proteomes between virulent m. tuberculosis h37rv (mtb) and the mycobacterium bovis bcg vaccine strain. to compare the membrane proteomes, we used lc-ms/ms analysis in combination with lab ... | 2013 | 24093440 |
| field-isolated genotypes of mycobacterium bovis vary in virulence and influence case pathology but do not affect outbreak size. | strains of many infectious agents differ in fundamental epidemiological parameters including transmissibility, virulence and pathology. we investigated whether genotypes of mycobacterium bovis (the causative agent of bovine tuberculosis, btb) differ significantly in transmissibility and virulence, combining data from a nine-year survey of the genetic structure of the m. bovis population in northern ireland with detailed records of the cattle population during the same period. we used the size of ... | 2013 | 24086351 |
| trial watch: toll-like receptor agonists for cancer therapy. | toll-like receptors (tlrs) have long been known for their ability to initiate innate immune responses upon exposure to conserved microbial components such as lipopolysaccharide (lps) and double-stranded rna. more recently, this family of pattern recognition receptors has been attributed a critical role in the elicitation of anticancer immune responses, raising interest in the development of immunochemotherapeutic regimens based on natural or synthetic tlr agonists. in spite of such an intense wa ... | 2013 | 24083080 |
| molecular characterization of mycobacterium bovis isolates from pastoral livestock at mikumi-selous ecosystem in the eastern tanzania. | domestic ruminants and wild ungulates can act as reservoir hosts for mycobacterium bovis, causative agent for bovine tuberculosis (btb) in animals and man. bovine tuberculosis (btb) is endemic in pastoral livestock in tanzania. there is lack of information on genotypic distribution of m. bovis in animals at wildlife-livestock interface areas and in wildlife conserved areas. the objectives of this study were to characterize and determine the spatial distribution of m. bovis isolates. tissue sampl ... | 2013 | 24080121 |
| first-time detection of mycobacterium bovis in livestock tissues and milk in the west bank, palestinian territories. | bovine tuberculosis, btb, is classified by the who as one of the seven neglected zoonontic diseases that cause animal health problems and has high potential to infect humans. in the west bank, btb was not studied among animals and the prevalence of human tuberculosis caused by m. bovis is unknown. therefore, the aim of this study was to estimate the prevalence of btb among cattle and goats and identify the molecular characteristics of btb in our area. | 2013 | 24069475 |
| vaccines against diseases transmitted from animals to humans: a one health paradigm. | this review focuses on the immunization of animals as a means of preventing human diseases (zoonoses). three frameworks for the use of vaccines in this context are described, and examples are provided of successes and failures. framework i vaccines are used for protection of humans and economically valuable animals, where neither plays a role in the transmission cycle. the benefit of collaborations between animal health and human health industries and regulators in developing such products is di ... | 2013 | 24060567 |
| micror-142-3p down-regulates irak-1 in response to mycobacterium bovis bcg infection in macrophages. | micrornas (mirnas) have been demonstrated to play a pivotal role in the regulation of target gene expression at the post-transcriptional level. in order to better understand the role of mirna in the immunological regulation of macrophages against mycobacterium bovis bcg infection, we explored the alteration of immune-related mirna profile in macrophage raw264.7 cells in response to bcg infection in this study. our results demonstrated that mir-142-3p was a potential to negatively regulate the pr ... | 2013 | 24053976 |
| spatial and temporal interactions between livestock and wildlife in south central spain assessed by camera traps. | the diversification of livestock farms into hunting estates in south central spain (scs) may impede the success of mycobacterium bovis eradication programmes by facilitating transmission between wildlife and livestock. in this observational study we aimed to provide information of relevance about the nature and frequency of interactions (observed visits to study points) between livestock (cattle and domestic pigs) and wildlife (wild boar and red deer). the study was conducted in an extensive cat ... | 2013 | 24050782 |
| [evaluation of the reliability of thiophen-2-carboxylic acid hydrazine result for the identification of mycobacterium bovis]. | to evaluate the reliability of thiophen-2-carboxylic acid hydrazine (tch) test for identification of mycobacterium bovis (m. bovis). | 2013 | 24047811 |
| immunological evaluation of a novel mycobacterium tuberculosis antigen, rv3117, absent in mycobacterium bovis bcg. | tuberculosis (tb) remains a global infectious disease. to investigate the value of a novel mycobacterium tuberculosis (m. tuberculosis) region of difference 5 (rd5)-encoded antigen, rv3117, in the development of effective immuno-diagnostics and vaccines against tb, the immune responses to the antigen were examined in human subjects, as well as in c57bl/6 mice. the results showed that rv3117 was able to evoke specific humoral and cellular immune responses. consistent with the results from the rd1 ... | 2013 | 24045507 |
| mycobacterium bovis infection in goats from the northeast region of brazil. | a total of 8,058 male and female mixed-breed goats and 1-4 years of age were slaughtered over a period of 7 months at the public slaughterhouse of patos city, paraíba state, in the northeast region of brazil; 822 animals were inspected for gross lesions of tuberculosis, and 12 (1.46%) had lesions suggestive of tuberculosis in the mammary gland, lungs, liver and mediastinal, mesenteric, submandibular, parotid and prescapular lymph nodes. presence of granulomatous lesions was confirmed in the subm ... | 2011 | 24031775 |
| tuberculosis determined by mycobacterium bovis in captive waterbucks (kobus ellipsiprymnus) in são paulo, brazil. | two waterbucks from são paulo zoo foundation exhibited respiratory symptoms in july 2004. after euthanasia, granulommas in lungs and mediastinic lymph nodes were observed. acid-fast bacilli isolated were identified as mycobacterium bovis spoligotype sb0121 by pra and spoligotyping. they were born and kept in the same enclosure with the same group, without any contact to other species housed in the zoo. this is the first detailed description of m. bovis infection in kobus ellipsiprymnus. | 2011 | 24031687 |
| potential application of new diagnostic methods for controlling bovine tuberculosis in brazil. | bovine tuberculosis, a chronic infection in cattle caused by mycobacterium bovis, remains an economic and public health problem for several countries. due to its economic impact on international trade, contagious nature, and implications for human health, global programs to eradicate the disease were implemented worldwide. those programs are based on slaughtering ppd-reactive animals. despite the national programs in brazil, complete eradication has not been achieved, and the disease remains, al ... | 2010 | 24031527 |
| detection of mycobacterium bovis dna in nasal swabs from tuberculous cattle by a multiplex pcr. | detection of tuberculosis in cattle relies on the intradermal tuberculin test (itt), but a definitive diagnosis requires identification of the pathogen after the animal is slaughtered. dna in nasal swabs from 50 cows was analyzed by m-pcr, targeting for the rvd1-rv2031c and is6110 sequences. m. bovis was identified in two of 34 tuberculous cows (5.9%). the use of mpcr of nasal swabs as an in vivo diagnostic tool for bovine tuberculosis is suggested. | 2010 | 24031509 |
| identification of mycobacterium bovis isolates by a multiplex pcr. | isolates from suggestive bovine tuberculosis lesions were tested by a multiplex polymerase chain reaction (m-pcr) targeting for rvd1rv2031c and is6110 sequences, specific for m. bovis and mycobacterium tuberculosis complex respectively. the m-pcr successfully identified as m. bovis 88.24% of the isolates. | 2009 | 24031349 |
| comparison of three decontamination methods for mycobacterium bovis isolation. | sixty samples of tissue fragments with lesions suggestive of tuberculosis from bovine abattoirs, kept in saturated solution of sodium borate, were subjected to four treatments: 4% naoh (petroff method), 12 % h2so4 and 1.5% hpc (1-hexadecylpyridinium chloride) decontamination, and physiological saline solution (control). the hpc method showed the lowest contamination rate (3%) when compared to control (88%, p<0.001), naoh (33%, p<0.001) and h2so4 (21.7%, p<0.002). regarding the isolation success, ... | 2008 | 24031209 |
| immunisation with id83 fusion protein induces antigen-specific cell mediated and humoral immune responses in cattle. | in this study we have investigated the potential of mycobacterial proteins as candidate subunit vaccines for bovine tuberculosis. in addition, we have explored the use of tlr-ligands as potential adjuvants in cattle. in vitro screening assays with whole blood from mycobacterium bovis-infected and bcg-vaccinated cattle demonstrated that fusion protein constructs were most commonly recognised, and the id83 fusion protein was selected for further immunisation studies. furthermore, glucopyranosyl li ... | 2013 | 24012566 |
| wild boar tuberculosis in iberian atlantic spain: a different picture from mediterranean habitats. | infections with mycobacterium bovis and closely related members of the mycobacterium tuberculosis complex (mtc) are shared between livestock, wildlife and sporadically human beings. wildlife reservoirs exist worldwide and can interfere with bovine tuberculosis (tb) eradication efforts. the eurasian wild boar (sus scrofa) is a mtc maintenance host in mediterranean iberia (spain and portugal). however, few systematic studies in wild boar have been carried out in atlantic regions. we describe the p ... | 2013 | 24010539 |
| [disseminated tuberculosis in an infant with ifng-il-12/il 23 axis defect]. | bcg vaccine contains low virulence mycobacterium bovis bacillus. in mexico it is given at birth with few reported adverse reactions in immunocompetent children; however, in immuno-compromised patients it can cause serious local or systemic adverse reactions. the most frequent related diseases include chronic granulomatous disease (cgd), human immunodeficiency virus infection (hiv), severe combined immunodeficiency (scid) and mendelian susceptibility to mycobacterial disease (msmd). at this time, ... | 2013 | 24007964 |
| mycobacteria-infected dendritic cells attract neutrophils that produce il-10 and specifically shut down th17 cd4 t cells through their il-10 receptor. | neutrophils participate in the control of mycobacterial infection both by directly eliminating bacilli and by interacting with macrophages and dendritic cells (dcs). despite host defenses, slow-growing mycobacteria can persist in the host for decades, mostly inside macrophages and dcs, and eventually destroy tissues after exacerbated inflammation. il-17a-driven neutrophil recruitment may participate in this process. we report that mouse bone marrow-derived dcs infected with live mycobacterium bo ... | 2013 | 23997221 |
| non-contiguous genome sequence of mycobacterium simiae strain dsm 44165(t.). | mycobacterium simiae is a non-tuberculosis mycobacterium causing pulmonary infections in both immunocompetent and imunocompromized patients. we announce the draft genome sequence of m. simiae dsm 44165(t). the 5,782,968-bp long genome with 65.15% gc content (one chromosome, no plasmid) contains 5,727 open reading frames (33% with unknown function and 11 orfs sizing more than 5000 -bp), three rrna operons, 52 trna, one 66-bp tmrna matching with tmrna tags from mycobacterium avium, mycobacterium t ... | 2013 | 23991261 |
| investigation of the effect of mycobacterium bovis infection on bovine neutrophils functions. | bovine tuberculosis is a disease in cattle caused by infection with mycobacterium bovis. the disease has posed significant economic losses and remains a public health hazard worldwide. interactions between m. bovis and bovine macrophages have been extensively characterized in various studies, while similar analyses in neutrophils, which are one of the other types of white blood cells in mammals, were often overlooked. neutrophils provide defense against all microbes and can present a diverse col ... | 2013 | 23988278 |
| inhibition of mycobacterial growth in vitro following primary but not secondary vaccination with mycobacterium bovis bcg. | despite the widespread use of the mycobacterium bovis bcg vaccine, there are more than 9 million new cases of tuberculosis (tb) every year, and there is an urgent need for better tb vaccines. tb vaccine candidates are selected for evaluation based in part on the detection of an antigen-specific gamma interferon (ifn-γ) response. the measurement of mycobacterial growth in blood specimens obtained from subjects immunized with investigational tb vaccines may be a better in vitro correlate of in viv ... | 2013 | 23986316 |
| development of an unbiased antigen-mining approach to identify novel vaccine antigens and diagnostic reagents for bovine tuberculosis. | previous experiments for the identification of novel diagnostic or vaccine candidates for bovine tuberculosis have followed a targeted approach, wherein specific groups of proteins suspected to contain likely candidates are prioritized for immunological assessment (for example, with in silico approaches). however, a disadvantage of this approach is that the sets of proteins analyzed are restricted by the initial selection criteria. in this paper, we describe a series of experiments to evaluate a ... | 2013 | 23986315 |
| splitting of a prevalent mycobacterium bovis spoligotype by variable-number tandem-repeat typing reveals high heterogeneity in an evolving clonal group. | mycobacterium bovis populations in countries with persistent bovine tuberculosis usually show a prevalent spoligotype with a wide geographical distribution. this study applied mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (miru-vntr) typing to a random panel of 115 m. bovis isolates that are representative of the most frequent spoligotype in the iberian peninsula, sb0121. vntr typing targeted nine loci: etr-a (alias vntr2165), etr-b (vntr2461), etr-d (miru4, vntr580), ... | 2013 | 23985914 |
| abdominal aortic aneurysmal and endovascular device infection with iliopsoas abscess caused by mycobacterium bovis as a complication of intravesical bacillus calmette-guérin therapy. | vascular complications after the intravesical instillation of bacillus calmette-guérin (bcg) therapy are extremely rare. we experienced a case of abdominal aortic aneurysmal infection excluded by a stent graft with an iliopsoas abscess after intravesical instillation of bcg therapy that required reoperation. five years ago, an 81-year-old man was diagnosed with transitional cell carcinoma of the bladder. after transurethral resection of the bladder tumor and intravesical bcg therapy, a radical c ... | 2013 | 23972639 |
| mycobacterial etiology of pulmonary tuberculosis and association with hiv infection and multidrug resistance in northern nigeria. | objective. data on pulmonary tuberculosis (tb) caused by mycobacterium tuberculosis (mtb) complex in nigeria are limited. we investigated species of mtb complex in tb cases from northern nigeria. methods. new tb suspects were enrolled, screened for hiv and their sputum samples were cultured after routine microscopy. genotypes mtbc and mtbdrplus were used to characterize the mtb complex species and their resistance to isoniazid and rifampicin. results. of the 1,603 patients enrolled, 375 (23%) ha ... | 2013 | 23970967 |
| comparison of 14 molecular assays for detection of mycobacterium tuberculosis complex in bronchoalveolar lavage fluid. | we compared 14 molecular assays for their ability to detect the mycobacterium tuberculosis complex in bronchoalveolar lavage fluid samples. three approaches were followed. first, by using dna from mycobacterium bovis bcg, we determined the detection limits of the assays using routine molecular methods. second, in order to determine the analytical sensitivities of the assays, we added one of four m. tuberculosis isolates with various numbers of the insertion sequence is6110 to n-acetyl-l-cysteine ... | 2013 | 23966510 |
| mycobacterium bovis bcg killed by extended freeze-drying induces an immunoregulatory profile and protects against atherosclerosis. | atherosclerosis is an inflammatory disease of the arterial wall that leads to myocardial infarction and stroke. regulatory t cells (tregs) and il-10 exert significant anti-atherogenic effects in experimental models of atherosclerosis by modulating vascular inflammation. we have previously shown that mycobacterium bovis bcg killed by extended freeze-drying (efd bcg) decreases lung and colon inflammation by recruiting il-10-producing tregs. therefore, the aim of this study was to investigate the e ... | 2014 | 23962000 |
| mechanism for recognition of an unusual mycobacterial glycolipid by the macrophage receptor mincle. | binding of the macrophage lectin mincle to trehalose dimycolate, a key glycolipid virulence factor on the surface of mycobacterium tuberculosis and mycobacterium bovis, initiates responses that can lead both to toxicity and to protection of these pathogens from destruction. crystallographic structural analysis, site-directed mutagenesis, and binding studies with glycolipid mimics have been used to define an extended binding site in the c-type carbohydrate recognition domain (crd) of bovine mincl ... | 2013 | 23960080 |
| comparison of pcr versus culture for detection of mycobacterium bovis after experimental inoculation of various matrices held under environmental conditions for extended periods. | the purpose of this study was to compare the performance of a molecular detection technique (nested pcr) with that of mycobacterial culture in the detection of mycobacterium bovis dna in a set of 687 samples of experimentally inoculated environmental substrates (hay, soil, corn, water) exposed to natural weather conditions in michigan. four replicates of each substrate were used; half were autoclaved for sterilization, all were inoculated with 50,000 cfu of m. bovis isolated from michigan livest ... | 2013 | 23956383 |
| wildlife hosts for mycobacterium bovis. | 2013 | 23956289 | |
| eimeria maxima: efficacy of recombinant mycobacterium bovis bcg expressing apical membrane antigen1 against homologous infection. | coccidiosis is one of the most important protozoan diseases and inflicts severe economic losses on the poultry industry. the aim of this study was to evaluate the capacity of bacillus calmette-guerin (bcg) to deliver apical membrane antigen1 (ama1) of eimeria maxima to stimulate specific cellular and humoral immune responses in chickens. day-old birds were immunized twice with rbcg/pmv261-ama1, rbcg/pmv361-ama1, or bcg via oral, intranasal, and subcutaneous routes and then orally challenged with ... | 2013 | 23949244 |
| [vesical tuberculosis after bacillus calmette-guerin intravesical instillation therapy for bladder cancer]. | an 82-year-old man who had undergone transurethral resection (tur) for superficial bladder cancer (pt1 g3) in june and luly 2011 received intravesical bacillus calmette-guerin therapy (80 mg weekly for 6 weeks) in september 2011. seven months later, a follow-up cystoscopy revealed a slowly growing torose lesion at the site of the previous tur. the lesion was removed by tur in june 2012. pathological examination showed an inflammatory response with small granulomatous lesions. the specimen staine ... | 2013 | 23945329 |
| exosomes carrying mycobacterial antigens can protect mice against mycobacterium tuberculosis infection. | approximately 2 billion people are infected with mycobacterium tuberculosis, the etiological agent of tuberculosis (tb), and an estimated 1.5 million individuals die annually from tb. presently, mycobacterium bovis bcg remains the only licensed tb vaccine; however, previous studies suggest its protective efficacy wanes over time and fails in preventing pulmonary tb. therefore, a safe and effective vaccine is urgently required to replace bcg or boost bcg immunizations. our previous studies reveal ... | 2013 | 23943377 |
| heparin-binding haemagglutinin, a new tool for the detection of latent mycobacterium tuberculosis infection in hemodialysis patients. | patients with end-stage renal disease (esrd) and latently infected with mycobacterium tuberculosis (ltbi) are at higher risk to develop tuberculosis (tb) than healthy subjects. interferon-gamma release assays (igras) were reported to be more sensitive than tuberculin skin tests for the detection of infected individuals in dialysis patients. | 2013 | 23940693 |
| the phylogeny and population structure of mycobacterium bovis in the british isles. | to further understand the epidemic of bovine tuberculosis in great britain, northern ireland and the republic of ireland, we identified 16 mutations that are phylogenetically informative for mycobacterium bovis strains from these regions. we determined the status of these mutations among a collection of 501 strains representing the molecular diversity found in these three regions of the british isles. the resulting linear phylogenies from each region were concordant, showing that the same lineag ... | 2013 | 23933404 |
| simultaneous detection of mycobacterium bovis and m. tuberculosis in an apparently immunocompetent patient. | 2013 | 23928172 | |
| subcutaneous administration of a 10-fold-lower dose of a commercial human tuberculosis vaccine, mycobacterium bovis bacillus calmette-guerin danish, induced levels of protection against bovine tuberculosis and responses in the tuberculin intradermal test similar to those induced by a standard cattle dose. | vaccination of cattle with a commercial human tuberculosis (tb) vaccine, mycobacterium bovis bacillus calmette-guérin (bcg) danish, at a dose equivalent to 5 human doses of bcg has protected these animals against tb in field and experimental trials. there is interest in determining whether a 10-fold-lower dose could still protect cattle but not induce a tuberculin intradermal test response. two groups of calves (n = 9/group) were vaccinated subcutaneously with a lyophilized bcg danish vaccine co ... | 2013 | 23925885 |
| effect of iron concentration on the expression and activity of catalase-peroxidases in mycobacteria. | mycobacterial catalases are known to exist in different isoforms. we studied the influence of iron concentration on the expression and activity of the different isoforms in mycobacterium bovis bcg, m. smegmatis, m. fortuitum, m. kansasii and m. vaccae by growing them under iron-sufficient (4 microg fe/ml) and iron-deficient (0.02 microg fe/ml) conditions. upon iron deprivation, significant differences were observed in the catalase/peroxidase activities in both quantitative spectrophotometric ass ... | 2005 | 23923578 |
| cd30 is required for activation of a unique subset of interleukin-17a-producing γδ t cells in innate immunity against mycobacterium bovis bacillus calmette-guerin infection. | interleukin-17a (il-17a)-producing γδ t cells are known to be activated following mycobacterium bovis bacillus calmette-guérin (bcg) infection. here, we show that cd30, a member of the tumor necrosis factor (tnf) receptor superfamily, is important for activation of il-17a-producing γδ t cells after bcg infection. vγ1(-) vγ4(-) γδ t cells preferentially expressing vγ6/vδ1 genes were identified as the major source of il-17a in the peritoneal cavity during the early stage of bcg infection. the numb ... | 2013 | 23918785 |
| concomitant fungal and mycobacterium bovis infections in beef cattle in kenya. | bovine tuberculosis is an important zoonosis and accurate diagnosis is important for its surveillance. post-mortem diagnosis may, however, be compromised by lesions caused by other pathogens. in an investigation on its prevalence in slaughter cattle in kenya, mycobacterium bovis and dimorphic fungi were inadvertently identified separately or concurrently in tuberculous lesions. beef carcasses were inspected for lesions in two abattoirs in nairobi. tissues with lesions were collected and transpor ... | 2013 | 23902371 |
| the aim2 inflammasome is involved in macrophage activation during infection with virulent mycobacterium bovis strain. | mycobacterium bovis, the causative agent of bovine tuberculosis, infects host macrophages and triggers production of the proinflammatory cytokine interleukin 1β (il-1β). the mechanism by which macrophages become activated and secrete il-1β in tuberculosis has not yet been elucidated. | 2013 | 23901081 |
| non-tuberculous mycobacteria in wild boar (sus scrofa) from southern spain: epidemiological, clinical and diagnostic concerns. | non-tuberculous mycobacteria (ntm) are widely distributed in the environment, particularly in wet soil, marshland, rivers or streams, but also are causative agents of a wide variety of infections in animals and humans. little information is available regarding the ntm prevalence in wildlife and their effects or significance in the bovine tuberculosis (btb) epidemiology and diagnosis. this research shows the most frequently ntm isolated in lymph nodes of wild boar (sus scrofa) from southern spain ... | 2015 | 23895110 |
| natural killer cell cytokine response to m. bovis bcg is associated with inhibited proliferation, increased apoptosis and ultimate depletion of nkp44(+)cd56(bright) cells. | mycobacterium bovis bcg, a live attenuated strain of m. bovis initially developed as a vaccine against tuberculosis, is also used as an adjuvant for immunotherapy of cancers and for treatment of parasitic infections. the underlying mechanisms are thought to rely on its immunomodulatory properties including the recruitment of natural killer (nk) cells. in that context, we aimed to study the impact of m. bovis bcg on nk cell functions. we looked at cytotoxicity, cytokine production, proliferation ... | 2013 | 23874793 |
| notch signaling regulates expression of mcl-1 and apoptosis in ppd-treated macrophages. | macrophages are cellular targets for infection by bacteria and viruses. the fate of infected macrophages plays a key role in determining the outcome of the host immune response. apoptotic cell death of macrophages is considered to be a protective host defense that eliminates pathogens and infected cells. in this study, we investigated the involvement of notch signaling in regulating apoptosis in macrophages treated with tuberculin purified protein derivative (ppd). murine bone marrow-derived mac ... | 2013 | 23872918 |