Publications

ongoing outbreak of invasive listeriosis due to serotype 1/2a listeria monocytogenes, ancona province, italy, january 2015 to february 2016.	in the first seven weeks of 2016, five serotype 1/2a listeria monocytogenes isolates were collected from patients with invasive listeriosis in ancona province in italy. these strains and six 1/2a isolates identified in 2015 in the same area were typed by eric-pcr and pfge. a clonal relationship, documented between the two sets of isolates, suggested a listeriosis outbreak in ancona that started most probably in 2015. investigation into the source of infection is still ongoing.	2016	27168589
listeria monocytogenes in different specimens from healthy red deer and wild boars.	in the past, listeria monocytogenes has been isolated from game feces and meat. however, less information is available on the occurrence of l. monocytogenes in other specimens originating from game animals. hence, the aim of this study was to get an overview of the occurrence and distribution of l. monocytogenes in game animals by characterization of isolates from different matrices. for that purpose, samples were collected from red deer (cervus elaphus), wild boars (sus scrofa), and feed during ...	2016	27159352
probiotic properties of lactic acid bacteria isolated from human milk.	the objective of this study was to identify and characterize lactic acid bacteria isolated from human milk, with an emphasis on their probiotic properties.	2016	27159339
endothelial cells use a formin-dependent phagocytosis-like process to internalize the bacterium listeria monocytogenes.	vascular endothelial cells act as gatekeepers that protect underlying tissue from blood-borne toxins and pathogens. nevertheless, endothelial cells are able to internalize large fibrin clots and apoptotic debris from the bloodstream, although the precise mechanism of such phagocytosis-like uptake is unknown. we show that cultured primary human endothelial cells (huvec) internalize both pathogenic and non-pathogenic listeria bacteria comparably, in a phagocytosis-like process. in contrast with pr ...	2016	27152864
the absence of a mature cell wall sacculus in stable listeria monocytogenes l-form cells is independent of peptidoglycan synthesis.	l-forms are cell wall-deficient variants of otherwise walled bacteria that maintain the ability to survive and proliferate in absence of the surrounding peptidoglycan sacculus. while transient or unstable l-forms can revert to the walled state and may still rely on residual peptidoglycan synthesis for multiplication, stable l-forms cannot revert to the walled form and are believed to propagate in the complete absence of peptidoglycan. l-forms are increasingly studied as a fundamental biological ...	2016	27149671
preliminary study on the effect of fermented cheese whey on listeria monocytogenes, escherichia coli o157:h7, and salmonella goldcoast populations inoculated onto fresh organic lettuce.	cheese whey fermented by an industrial starter consortium of lactic acid bacteria was evaluated for its antibacterial capacity to control a selection of pathogenic bacteria. for their relevance on outbreak reports related to vegetable consumption, this selection included listeria monocytogenes, serotype 4b, escherichia coli o157:h7, and salmonella goldcoast. organically grown lettuce was inoculated with an inoculum level of ∼10(7) colony-forming unit (cfu)/ml and was left for about 1 h in a safe ...	2016	27149657
use of lactobacillus plantarum strains as a bio-control strategy against food-borne pathogenic microorganisms.	lactobacillus plantarum is one of the most versatile species extensively used in the food industry both as microbial starters and probiotic microorganisms. several l. plantarum strains have been shown to produce different antimicrobial compounds such as organic acids, hydrogen peroxide, diacetyl, and also bacteriocins and antimicrobial peptides, both denoted by a variable spectrum of action. in recent decades, the selection of microbial molecules and/or bacterial strains able to produce antagoni ...	2016	27148172
antimicrobial hyaluronic acid-cefoxitin sodium thin films produced by electrospraying.	the healing properties of hyaluronic acid (ha) in the recovery of wounds are well known. cefoxitin (cef), a cephalosporin antibiotic, is generally used to prevent and treat postoperative infections. in this study, we describe the incorporation of cef in ha thin films (cef-haf) by using electrospraying. scanning electron microscopy images showed that ha-containing thin films (haf) were composed of numerous nanoparticles (255 ± 177 nm in diameter) with irregular surfaces, connected to each other w ...	2016	27146506
biochemical properties and mechanism of action of enterocin ld3 purified from enterococcus hirae ld3.	enterocin ld3 was purified using activity-guided multistep chromatography techniques such as cation-exchange and gel-filtration chromatography. the preparation's purity was tested using reverse-phase ultra-performance liquid chromatography. the specific activity was tested to be 187.5 au µg(-1) with 13-fold purification. purified enterocin ld3 was heat stable up to 121 °c (at 15 psi pressure) and ph 2-6. the activity was lost in the presence of papain, reduced by proteinase k, pepsin and trypsin ...	2016	27145777
two listeria outbreaks caused by smoked fish consumption-using whole-genome sequencing for outbreak investigations.	listeria monocytogenes may contaminate and persist in food production facilities and cause repeated, seemingly sporadic, illnesses over extended periods of time. we report on the investigation of two such concurrent outbreaks. we compared patient isolates and available isolates from foods and food production facilities by use of whole-genome sequencing and subsequent multilocus sequence type and single nucleotide polymorphism analysis. outbreak cases shared outbreak strains, defined as listeria ...	2016	27145209
bacteriocin from epidemic listeria strains alters the host intestinal microbiota to favor infection.	listeria monocytogenes is responsible for gastroenteritis in healthy individuals and for a severe invasive disease in immunocompromised patients. among the three identified l. monocytogenes evolutionary lineages, lineage i strains are overrepresented in epidemic listeriosis outbreaks, but the mechanisms underlying the higher virulence potential of strains of this lineage remain elusive. here, we demonstrate that listeriolysin s (lls), a virulence factor only present in a subset of lineage i stra ...	2016	27140611
anti-infective activity of 2-cyano-3-acrylamide inhibitors with improved drug-like properties against two intracellular pathogens.	due to the rise of antibiotic resistance and the small number of effective antiviral drugs, new approaches for treating infectious diseases are urgently needed. identifying targets for host-based therapies represents an emerging strategy for drug discovery. the ubiquitin-proteasome system is a central mode of signaling in the eukaryotic cell and may be a promising target for therapies that bolster the host's ability to control infection. deubiquitinase (dub) enzymes are key regulators of the hos ...	2016	27139470
characterization of two listeria innocua chitinases of different sizes that were expressed in escherichia coli.	two putative chitinase genes, lin0153 and lin1996, from the nonpathogenic bacterium listeria innocua were expressed in escherichia coli, and the gene products were characterized. the genes were close homologs of chitinases from the pathogenic bacterium listeria monocytogenes, in which chitinases and chitin-binding proteins play important roles in pathogenesis in mice-infection models. the purified recombinant enzymes that are different in size, linchi78 (lin0153 product) and linchi35 (lin1996 pr ...	2016	27138200
blue-light inhibition of listeria monocytogenes growth is mediated by reactive oxygen species and is influenced by σb and the blue-light sensor lmo0799.	listeria monocytogenes senses blue light via the flavin mononucleotide-containing sensory protein lmo0799, leading to activation of the general stress response sigma factor sigb (σ(b)). in this study, we investigated the physiological response of this foodborne pathogen to blue light. we show that blue light (460 to 470 nm) doses of 1.5 to 2 mw cm(-2) cause inhibition of growth on agar-based and liquid culture media. the inhibitory effects are dependent on cell density, with reduced effects evid ...	2016	27129969
characteristics and distribution of listeria spp., including listeria species newly described since 2009.	the genus listeria is currently comprised of 17 species, including 9 listeria species newly described since 2009. genomic and phenotypic data clearly define a distinct group of six species (listeria sensu strictu) that share common phenotypic characteristics (e.g., ability to grow at low temperature, flagellar motility); this group includes the pathogen listeria monocytogenes. the other 11 species (listeria sensu lato) represent three distinct monophyletic groups, which may warrant recognition a ...	2016	27129530
[increased apoptosis and down-regulation of rhoa in hepg2 cells infected by listeria monocytogenes].	to explore the apoptosis of hepg2 cells infected by listeria monocytogenes egd strain (lm-egd) as well as rho family small gtpases rhoa expression.	2016	27126939
interrupting cd28 costimulation before antigen rechallenge affects cd8(+) t-cell expansion and effector functions during secondary response in mice.	the role of cd28-mediated costimulation in secondary cd8(+) t-cell responses remains controversial. here, we have used two tools - blocking mouse anti-mouse cd28-specific antibodies and inducible cd28-deleting mice - to obtain definitive answers in mice infected with ovalbumin-secreting listeria monocytogenes. we report that both blockade and global deletion of cd28 reveal its requirement for full clonal expansion and effector functions such as degranulation and ifn-γ production during the secon ...	2016	27122236
term-seq reveals abundant ribo-regulation of antibiotics resistance in bacteria.	riboswitches and attenuators are cis-regulatory rna elements, most of which control bacterial gene expression via metabolite-mediated, premature transcription termination. we developed an unbiased experimental approach for genome-wide discovery of such ribo-regulators in bacteria. we also devised an experimental platform that quantitatively measures the in vivo activity of all such regulators in parallel and enables rapid screening for ribo-regulators that respond to metabolites of choice. using ...	2016	27120414
restraining reactive oxygen species in listeria monocytogenes promotes the apoptosis of glial cells.	listeria monocytogenes is a facultative anaerobic foodborne pathogen that can traverse the blood-brain barrier and cause brain infection. l. monocytogenes infection induces host cell apoptosis in several cell types. in this study, we investigated the apoptosis of human glioma cell line u251 invaded by l. monocytogenes and evaluated the function of bacterial reactive oxygen species (ros) during infection.	2016	27120397
the continuous challenge of characterizing the foodborne pathogen listeria monocytogenes.	listeria monocytogenes is an important foodborne pathogen commonly isolated from food processing environments and food products. this organism can multiply at refrigeration temperatures, form biofilms on different materials and under various conditions, resist a range of environmental stresses, and contaminate food products by cross-contamination. l. monocytogenes is recognized as the causative agent of listeriosis, a serious disease that affects mainly individuals from high-risk groups, such as ...	2016	27120361
the microbiological quality of pasteurized milk sold by automatic vending machines.	the microbiological quality of pasteurized milk samples (n = 39) collected during 13 weekly intervals from three automatic vending machines (avm) in greece was investigated. microbiological counts (total aerobic (tac), total psychrotrophic (tpc), enterobacteriaceae (ec), and psychrotrophic aerobic bacterial spore counts (pabsc)) were obtained at the time of sampling and at the end of shelf-life (3 days) after storage of the samples at 4 or 8°c. tac were found to be below the 10(7 ) cfu ml(-1) li ...	2016	27116619
cellulose aerogels functionalized with polypyrrole and silver nanoparticles: in-situ synthesis, characterization and antibacterial activity.	green porous and lightweight cellulose aerogels have been considered as promising candidates to substitute some petrochemical host materials to support various nanomaterials. in this work, waste wheat straw was collected as feedstock to fabricate cellulose hydrogels, and a green inexpensive naoh/polyethylene glycol solution was used as cellulose solvent. prior to freeze-drying treatment, the cellulose hydrogels were integrated with polypyrrole and silver nanoparticles by easily-operated in-situ ...	2016	27112885
preparations and characterization of alginate/silver composite films: effect of types of silver particles.	alginate-based films reinforced with different types of silver particles such as metallic silver (agm), silver zeolite (agz), citrate reduced silver nanoparticles (agnp(c)), laser ablated silver nanoparticles (agnp(la)), and silver nitrate (agno3) were prepared using a solvent casting method and the effect of silver particles on the optical, mechanical, water vapor barrier, and antimicrobial properties the composite films was evaluated. size and shape of the silver particles were varied dependin ...	2016	27112867
fully automated and colorimetric foodborne pathogen detection on an integrated centrifugal microfluidic device.	this work describes fully automated and colorimetric foodborne pathogen detection on an integrated centrifugal microfluidic device, which is called a lab-on-a-disc. all the processes for molecular diagnostics including dna extraction and purification, dna amplification and amplicon detection were integrated on a single disc. silica microbeads incorporated in the disc enabled extraction and purification of bacterial genomic dna from bacteria-contaminated milk samples. we targeted four kinds of fo ...	2016	27112702
potential antimicrobial and antiproliferative activities of autochthonous starter cultures and protease epg222 in dry-fermented sausages.	this work studied the presence of nitrogen compounds with bioactive properties in iberian pork sausages that were manufactured using different autochthonous starter cultures (pediococcus acidilactici ms200 and staphylococcus vitulus rs34) and protease epg222. nitrogen compounds were extracted and evaluated for their antimicrobial effect against spoilage and pathogenic bacteria, such as bacillus cereus, escherichia coli, salmonella choleraesuis, staphylococcus aureus and listeria monocytogenes, a ...	2016	27112426
click-em for imaging metabolically tagged nonprotein biomolecules.	em has long been the main technique for imaging cell structures with nanometer resolution but has lagged behind light microscopy in the crucial ability to make specific molecules stand out. here we introduce click-em, a labeling technique for correlative light microscopy and em imaging of nonprotein biomolecules. in this approach, metabolic labeling substrates containing bioorthogonal functional groups are provided to cells for incorporation into biopolymers by endogenous biosynthetic machinery. ...	2016	27110681
listeria monocytogenes infection differentially affects expression of ligands for nk cells and nk cell responses, depending on the cell type infected.	the pivotal role of nk cells in viral infection is extensively studied, whereas the role of nk cells in bacterial infection has been poorly investigated. here, we have examined how listeria monocytogenes (lm) affects expression of ligands for nk cell receptors and subsequent nk cell responses, depending on the type of cell infected. lm infected rat cell lines derived from different tissues were coincubated with splenic nk cells, and nk cell proliferation and ifn-γ production were measured. in ad ...	2016	27106671
rifampicin- and rifabutin-resistant listeria monocytogenes strains isolated from food products carry mutations in rpob gene.	the aim of this study was to investigate the mechanism of rifampicin resistance in listeria monocytogenes strains isolated from different types of food and the impact of specific mutations in the rpob gene on susceptibility to different antimicrobial agents and on fitness cost. fifteen spontaneous rifampicin-resistant strains were selected. the dna regions corresponding to clusters i-ii, iii, and n-terminal end of the rpob gene of escherichia coli were amplified and sequenced, leading to the ide ...	2016	27105395
listeriolysin o membrane damaging activity involves arc formation and lineaction -- implication for listeria monocytogenes escape from phagocytic vacuole.	listeriolysin-o (llo) plays a crucial role during infection by listeria monocytogenes. it enables escape of bacteria from phagocytic vacuole, which is the basis for its spread to other cells and tissues. it is not clear how llo acts at phagosomal membranes to allow bacterial escape. the mechanism of action of llo remains poorly understood, probably due to unavailability of suitable experimental tools that could monitor llo membrane disruptive activity in real time. here, we used high-speed atomi ...	2016	27104344
rifampin phosphotransferase is an unusual antibiotic resistance kinase.	rifampin (rif) phosphotransferase (rph) confers antibiotic resistance by conversion of rif and atp, to inactive phospho-rif, amp and pi. here we present the crystal structure of rph from listeria monocytogenes (rph-lm), which reveals that the enzyme is comprised of three domains: two substrate-binding domains (atp-grasp and rif-binding domains); and a smaller phosphate-carrying his swivel domain. using solution small-angle x-ray scattering and mutagenesis, we reveal a mechanism where the swivel ...	2016	27103605
chemical composition, antioxidant and antibacterial activities of essential oils from ferulago angulata.	ferulago angulata boiss. (apiaceae), a perennial aromatic herb, grows wild in iran. the aerial parts of f. angulata are used as a flavouring in foods, especially dairy foods by indigenous people in western and southwestern iran.	2016	27102982
cip2a promotes t-cell activation and immune response to listeria monocytogenes infection.	the oncoprotein cancerous inhibitor of protein phosphatase 2a (cip2a) is overexpressed in most malignancies and is an obvious candidate target protein for future cancer therapies. however, the physiological importance of cip2a-mediated pp2a inhibition is largely unknown. as pp2a regulates immune responses, we investigated the role of cip2a in normal immune system development and during immune response in vivo. we show that cip2a-deficient mice (cip2ahoz) present a normal immune system developmen ...	2016	27100879
in vivo efficacy of trans-cinnamaldehyde, carvacrol, and thymol in attenuating listeria monocytogenes infection in a galleria mellonella model.	listeria monocytogenes is a major foodborne pathogen that causes life-threatening illnesses in humans. with emergence of antibiotic resistance in l. monocytogenes, there is considerable interest in testing the efficacy of alternative therapies for controlling listeriosis in humans. this study investigated the efficacy of three phytochemicals, namely trans-cinnamaldehyde (tc), carvacrol (cr), and thymol (ty) in reducing l. monocytogenes virulence in the recently established invertebrate model, ga ...	2016	27094514
inhibition of listeria monocytogenes in hot dogs by surface application of freeze-dried bacteriocin-containing powders from lactic acid bacteria.	six lactic acid bacteria (lab) strains, lactococcus lactis bfe 920, l. lactis subsp. lactis atcc 11454, l. lactis subsp. cremoris atcc 14365, lactobacillus curvatus l442, lact. curvatus lth 1174, and lact. bavaricus mn, were grown in cheddar cheese whey supplemented with complex nutrient sources. cell-free culture supernatants were freeze-dried, and the resulting bacteriocin-containing powders were applied on the surface of hot dogs that were inoculated (~4 log cfu/hot dog) with a five-strain li ...	2016	27094263
previous homologous and heterologous stress exposure induces tolerance development to pulsed light in listeria monocytogenes.	as one of the emerging non-thermal technologies, pulsed light (pl) facilitates rapid, mild and residue-free microbial surface decontamination of food and food contact materials. while notable progress has been made in the characterization of the inactivation potential of pl, experimental data available on the tolerance development to the same (homologous) stress or to different (heterologous) stresses commonly applied in food manufacturing (e.g., acid, heat, salt) is rather controversial. the fi ...	2016	27092137
biocontrol and rapid detection of food-borne pathogens using bacteriophages and endolysins.	bacteriophages have been suggested as natural food preservatives as well as rapid detection materials for food-borne pathogens in various foods. since listeria monocytogenes-targeting phage cocktail (listshield) was approved for applications in foods, numerous phages have been screened and experimentally characterized for phage applications in foods. a single phage and phage cocktail treatments to various foods contaminated with food-borne pathogens including e. coli o157:h7, salmonella enterica ...	2016	27092128
implementation of nationwide real-time whole-genome sequencing to enhance listeriosis outbreak detection and investigation.	listeria monocytogenes (lm) causes severe foodborne illness (listeriosis). previous molecular subtyping methods, such as pulsed-field gel electrophoresis (pfge), were critical in detecting outbreaks that led to food safety improvements and declining incidence, but pfge provides limited genetic resolution. a multiagency collaboration began performing real-time, whole-genome sequencing (wgs) on all us lm isolates from patients, food, and the environment in september 2013, posting sequencing data i ...	2016	27090985
agricultural by-products with bioactive effects: a multivariate approach to evaluate microbial and physicochemical changes in a fresh pork sausage enriched with phenolic compounds from olive vegetation water.	the use of phenolic compounds derived from agricultural by-products could be considered as an eco-friendly strategy for food preservation. in this study a purified phenol extract from olive vegetation water (peovw) was explored as a potential bioactive ingredient for meat products using italian fresh sausage as food model. the research was developed in two steps: first, an in vitro delineation of the extract antimicrobial activities was performed, then, the peovw was tested in the food model to ...	2016	27088870
how many parameters does it take to describe disease tolerance?	the study of infectious disease has been aided by model organisms, which have helped to elucidate molecular mechanisms and contributed to the development of new treatments; however, the lack of a conceptual framework for unifying findings across models, combined with host variability, has impeded progress and translation. here, we fill this gap with a simple graphical and mathematical framework to study disease tolerance, the dose response curve relating health to microbe load; this approach hel ...	2016	27088212
a molluscan calreticulin ortholog from haliotis discus discus: molecular characterization and transcriptional evidence for its role in host immunity.	calreticulin (calr), a ca(2+) binding chaperone of the endoplasmic reticulum (er) and mainly involved in ca(2+) storage and signaling. in this study, we report the molecular characterization and immune responses of calr homolog from disk abalone (abcalr). the full length abcalr cdna (1837 bp) had an orf of 1224 bp. according to the multiple alignments analysis, n- and p-domains were highly conserved in all the selected members of calrs. in contrast, the c-domain which terminated with the charact ...	2016	27086846
how nacl and water content determine water activity during ripening of gouda cheese, and the predicted effect on inhibition of listeria monocytogenes.	this study describes the diffusion of nacl and water in gouda cheese during brining and ripening. furthermore, we established water activity as a function of the nacl-in-moisture content in gouda cheese during ripening. we determined nacl content, water content, and water activity in block-type gouda cheeses that were brined for 3.8d and foil-ripened for a period of 26 wk, and in wheel-type gouda cheeses that were brined for 0.33, 2.1, or 8.9d and subsequently nature-ripened for a period of 26 w ...	2016	27085417
listeria monocytogenes remodels the cell surface in the blood-stage.	after crossing the intestinal barrier, the bacterial pathogen listeria monocytogenes disseminates via the blood to the liver, spleen, brain and placenta. transcriptomic studies have shown that l. monocytogenes changes expression of many genes during this blood-stage. however, no comparable data at the protein level are known. as main interactors with the environment, we focused in surface proteins produced by l. monocytogenes in an ex vivo bovine blood model. bacteria exposed to blood alter sele ...	2016	27085096
increased biomass production by mesophilic food-associated bacteria through lowering the growth temperature from 30°c to 10°c.	five isolates from chilled food and refrigerator inner surfaces and closely related reference strains of the species escherichia coli, listeria monocytogenes, staphylococcus xylosus, bacillus cereus, pedobacter nutrimenti, and pedobacter panaciterrae were tested for the effect of growth temperature (30°c and 10°c) on biomass formation. growth was monitored via optical density, and biomass formation was measured at the early stationary phase based on the following parameters in complex and define ...	2016	27084015
simultaneous detection of waddlia chondrophila and listeria monocytogenes in aborted ruminant samples by real-time quantitative pcr.		2016	27083520
international interlaboratory study comparing single organism 16s rrna gene sequencing data: beyond consensus sequence comparisons.	this study presents the results from an interlaboratory sequencing study for which we developed a novel high-resolution method for comparing data from different sequencing platforms for a multi-copy, paralogous gene. the combination of pcr amplification and 16s ribosomal rna gene (16s rrna) sequencing has revolutionized bacteriology by enabling rapid identification, frequently without the need for culture. to assess variability between laboratories in sequencing 16s rrna, six laboratories sequen ...	2015	27077030
proteolysis triggers self-assembly and unmasks innate immune function of a human α-defensin peptide.	human α-defensin 6 (hd6) is a unique peptide of the defensin family that provides innate immunity in the intestine by self-assembling to form high-order oligomers that entrap bacteria and prevent host cell invasion. here, we report critical steps in the self-assembly pathway of hd6. we demonstrate that hd6 is localized in secretory granules of small intestinal paneth cells. hd6 is stored in these granules as an 81-residue propeptide (prohd6), and is recovered from ileal lumen as a 32-residue mat ...	2016	27076903
validation of the applied biosystems 7500 fast instrument for detection of <em>listeria monocytogenes</em> with the suretect <em>listeria monocytogenes</em> pcr assay.	in 2013, the thermo scientific™ suretect™ listeria monocytogenes real-time pcr assay was certified by the aoac research institute (ri) performance tested methods (sm) program as a rapid method for the detection of l. monocytogenes from a wide range of food matrixes and surface samples. this report details the method modification studies undertaken to extend the analysis of this pcr assay to the applied biosystems™ 7500 fast pcr instrument and applied biosystems rapidfinder™ express 2.0 software ...	2016	27075433
il-12p40/il-10 producing precd8α/clec9a+ dendritic cells are induced in neonates upon listeria monocytogenes infection.	infection by listeria monocytogenes (lm) causes serious sepsis and meningitis leading to mortality in neonates. this work explored the ability of cd11c(high) lineage dcs to induce cd8+ t-cell immune protection against lm in mice before 7 days of life, a period symbolized by the absence of murine il-12p70-producing cd11c(high)cd8α+ dendritic cells (dcs). we characterized a dominant functional batf3-dependent precursor of cd11c(high) dcs that is clec9a+cd205+cd24+ but cd8α- at 3 days of life. afte ...	2016	27074026
picoliter well array chip-based digital recombinase polymerase amplification for absolute quantification of nucleic acids.	absolute, precise quantification methods expand the scope of nucleic acids research and have many practical applications. digital polymerase chain reaction (dpcr) is a powerful method for nucleic acid detection and absolute quantification. however, it requires thermal cycling and accurate temperature control, which are difficult in resource-limited conditions. accordingly, isothermal methods, such as recombinase polymerase amplification (rpa), are more attractive. we developed a picoliter well a ...	2016	27074005
microbiological quality of the water used in agriculture in sardinia.	the microbiological quality of the water used in irrigation is crucial for the safety of products, such as fruit and vegetables, especially when destined to be consumed raw. however, the microbiological quality of this water is not defined at a community regulatory level or at a national level.	2016	27071326
inhibition of listeria monocytogenes in fresh cheese using chitosan-grafted lactic acid packaging.	a chitosan from biologically obtained chitin was successfully grafted with d,l-lactic acid (la) in aqueous media using p-toluenesulfonic acid as catalyst to obtain a non-toxic, biodegradable packaging material that was characterized using scanning electron microscopy, water vapor permeability, and relative humidity (rh) losses. additionally, the grafting in chitosan with la produced films with improved mechanical properties. this material successfully extended the shelf life of fresh cheese and ...	2016	27070568
matrix metalloproteinase-9 plays a role in protecting zebrafish from lethal infection with listeria monocytogenes by enhancing macrophage migration.	zebrafish could serve as an alternative animal model for pathogenic bacteria in multiple infectious routes. our previous study showed that immersion infection in zebrafish with listeria monocytogenes did not cause lethality but induced transient expression of several immune response genes. we used an affymetrix gene chip to examine the expression profiles of genes of zebrafish immersion-infected with l. monocytogenes. a total of 239 genes were up-regulated and 56 genes down-regulated compared wi ...	2016	27068748
the metalloprotease mpl supports listeria monocytogenes dissemination through resolution of membrane protrusions into vacuoles.	listeria monocytogenes is an intracellular pathogen that disseminates within the intestinal epithelium through acquisition of actin-based motility and formation of plasma membrane protrusions that project into adjacent cells. the resolution of membrane protrusions into vacuoles from which the pathogen escapes results in bacterial spread from cell to cell. this dissemination process relies on the mlp-acta-plcb operon, which encodes acta, a bacterial nucleation-promoting factor that mediates actin ...	2016	27068088
role of host type ia phosphoinositide 3-kinase pathway components in invasin-mediated internalization of yersinia enterocolitica.	many bacterial pathogens subvert mammalian type ia phosphoinositide 3-kinase (pi3k) in order to induce their internalization into host cells. how pi3k promotes internalization is not well understood. also unclear is whether type ia pi3k affects different pathogens through similar or distinct mechanisms. here, we performed an rna interference (rnai)-based screen to identify components of the type ia pi3k pathway involved in invasin-mediated entry of yersinia enterocolitica, an enteropathogen that ...	2016	27068087
isolation, identification, and characterization of listeria spp. from various animal origin foods.	the present study was undertaken with the prime objective of isolating and identifying listeria spp. from various foods of animal origin sold at retail market outlets in the city of navsari, gujarat.	2015	27065632
role of proapoptotic bh3-only proteins in listeria monocytogenes infection.	the ability of pathogens to influence host cell survival is a crucial virulence factor. listeria monocytogenes (lm) infection is known to be associated with severe apoptosis of hepatocytes and spleen cells. this impairs host defense mechanisms and thereby facilitates the spread of intracellular pathogens. the general mechanisms of apoptosis elicited by lm infection are understood, however, the roles of bh3-only proteins during primary lm infection have not been examined. to explore the roles of ...	2016	27064265
an experimental platform using human intestinal epithelial cell lines to differentiate between hazardous and non-hazardous proteins.	human intestinal epithelial cell lines (t84, caco-2, and hct-8) grown on permeable transwell™ filters serve as models of the gastrointestinal barrier. in this study, this in vitro model system was evaluated for effectiveness at distinguishing between hazardous and non-hazardous proteins. indicators of cytotoxicity (ldh release, mtt conversion), monolayer barrier integrity ([(3)h]-inulin flux, horseradish peroxidase flux, trans-epithelial electrical resistance [teer]), and inflammation (il-8, il- ...	2016	27060235
characterization of antimicrobial resistance of listeria monocytogenes strains isolated from a pork processing plant and its respective meat markets in southern china.	a total of 78 listeria monocytogenes isolates from a pork processing plant and the respective meat markets in southern china were examined. this number includes 60 isolates from pork at markets, 5 from cooked pork products at markets, 10 from pork at a processing plant, and 3 from food-contact surfaces at the processing plant. all isolates were subjected to serotyping, antimicrobial susceptibility testing, pulsed-field gel electrophoresis (pfge), and molecular basis of antibiotic resistance. fou ...	2016	27058266
listeria monocytogenes merr-like regulator nmlrlm: its transcriptome and role in stress response.	nmlr, a negative transcription regulator in the merr family, is involved in oxidative and nitrosative stress response in neisseria gonorrhoeae and haemophilus influenzae. in this study, the objective was to characterize the role and the regulon of nmlr in the foodborne listeria monocytogenes. an l. monocytogenes nmlr null mutant strain was constructed. transcriptomes of strain 10403s wild type (wt) and δnmlrlm strains grown to the stationary phase were determined by mrna sequencing. differential ...	2016	27058117
genome sequences for a cluster of human isolates of listeria monocytogenes identified in south africa in 2015.	listeria monocytogenesis a gram-positive bacterium with a ubiquitous presence in the environment. there is growing concern about the increasing prevalence ofl. monocytogenesassociated with food-borne outbreaks. here we report genome sequences for a cluster of human isolates ofl. monocytogenesidentified in south africa in 2015.	2016	27056221
listeria meningitis in an immunocompetent child: a case report.	listeria monocytogenes is a facultative anerobic, gram-positive bacillus that is isolated from the soil, vegetables, and wild or domestic animals. listeria infection is usually found in the older adults, immunocompromised patients, pregnant women, and newborns, whereas it is rare in healthy infants and children. listeria monocytogenes may cause meningitis, meningoencephalitis, brain abscess, pyogenic arthritis, osteomyelitis, and liver abscess in children. the course of meningoencephalitis by li ...	2016	27055168
hygiene and safety in the meat processing environment from butcher shops: microbiological contamination and listeria monocytogenes.	the quality and safety of meat products can be estimated by assessing their contamination by hygiene indicator microorganisms and some foodborne pathogens, with listeria monocytogenes as a major concern. to identify the main sources of microbiological contamination in the processing environment of three butcher shops, surface samples were obtained from the hands of employees, tables, knives, inside butcher displays, grinders, and meat tenderizers (24 samples per point). all samples were subjecte ...	2016	27052868
viability of listeria monocytogenes on boneless, water-added hams, commercially prepared with and without food-grade chemicals, during extended storage at 4 and/or -2.2°c.	viability of listeria monocytogenes was monitored during refrigerated (4°c) and/or frozen (i.e., deep chilling at -2.2°c) storage on casing-cooked hams that were commercially prepared with and without potassium lactate and sodium diacetate (1.6%), buffered vinegar (2.2%), buffered vinegar and potassium lactate (1.7%), or a blend of potassium lactate, potassium acetate, and sodium diacetate (1.7%). a portion of these hams were subsequently surface treated with lauric arginate ester (lae; 44 ppm). ...	2016	27052866
assessment of the microbiological safety of precut fruit from retail and catering premises in the united kingdom.	fresh fruit has been associated with a number of foodborne outbreaks in recent years. in particular, a large outbreak of listeriosis in the united states in 2011 was associated with consumption of cantaloupe melon, and an outbreak of salmonella newport in the united kingdom and europe (also in 2011) was linked to watermelon consumption. a study of precut fruit products from catering and retail premises in the united kingdom was, therefore, carried out to assess their microbiological safety. betw ...	2016	27052864
effectiveness of broad-spectrum chemical produce sanitizers against foodborne pathogens as in vitro planktonic cells and on the surface of whole cantaloupes and watermelons.	over the past few years, foodborne disease outbreaks linked to enteric pathogens present on cantaloupe and watermelon surfaces have raised concerns in the melon industry. this research evaluated the effectiveness of commercially available produce sanitizers against selected foodborne pathogens, both in cell suspensions and on the outer rind surface of melons. the sanitizers (65 and 200 ppm of chlorine, 5 and 35% hydrogen peroxide, 5 and 50 ppm of liquid chlorine dioxide, various hydrogen peroxid ...	2016	27052854
efficacy of uv-c irradiation for inactivation of food-borne pathogens on sliced cheese packaged with different types and thicknesses of plastic films.	in this study, the efficacy of using uv-c light to inactivate sliced cheese inoculated with escherichia coli o157:h7, salmonella typhimurium, and listeria monocytogenes and, packaged with 0.07 mm films of polyethylene terephthalate (pet), polyvinylchloride (pvc), polypropylene (pp), and polyethylene (pe) was investigated. the results show that compared with pet and pvc, pp and pe films showed significantly reduced levels of the three pathogens compared to inoculated but non-treated controls. the ...	2016	27052716
microbial and sensorial models for head-on and gutted (hog) atlantic salmon (salmo salar) stored from 0 to 15 °c.	predictive models offer efficient means to manage the quality and safety of highly perishable seafood. salmon is an increasingly popular seafood, and relies on well managed domestic and international supply chains to minimize growth of spoilage and pathogenic bacteria. while the literature describes predictive models for smoked and modified atmosphere packaged salmon, there are no reported models for spoilage bacteria and listeria monocytogenes on head-on and gutted (hog) aerobically-stored atla ...	2016	27052713
metagenomic analysis of a mexican ripened cheese reveals a unique complex microbiota.	cotija cheese is a mexican handcrafted product made from raw cow milk whose ripening process occurs spontaneously and, presumably, it is influenced by environmental conditions. its sensory characteristics and safety are probably the result of the balance between microbial populations and their metabolic capacity. in this work, we studied the dominance and richness of the bacteria in the cotija cheese microbiome, as well as their metabolic potential by high-throughput sequencing. by the analysis ...	2016	27052710
persistent and non-persistent strains of listeria monocytogenes: a focus on growth kinetics under different temperature, salt, and ph conditions and their sensitivity to sanitizers.	this study aimed to investigate the effect of different conditions, including temperature (37 °c, 22 °c, and 4 °c), nacl concentrations (2.5%, 4%, and 8%), and acidity (ph = 5), on the growth response of persistent and non-persistent isolates of listeria monocytogenes. the resistance to two common sanitizers (benzalkonium chloride and hydrogen peroxide) was also investigated. a selected group of 41 persistent and non-persistent l. monocytogenes isolates recovered from three cheese processing pla ...	2016	27052708
inhibition of listeria monocytogenes on cooked cured chicken breasts by acidified coating containing allyl isothiocyanate or deodorized oriental mustard extract.	ready-to-eat meats are considered foods at high risk to cause life-threatening listeria monocytogenes infections. this study screened 5 l. monocytogenes strains for their ability to hydrolyze sinigrin (a glucosinolate in oriental mustard), which formed allyl isothiocyanate (aitc) and reduced l. monocytogenes viability on inoculated vacuum-packed, cooked, cured roast chicken slices at 4 °c. tests involved incorporation of 25-50 μl/g aitc directly or 100-250 mg/g oriental mustard extract in 0.5% ( ...	2016	27052706
h3k4me3 demethylase kdm5a is required for nk cell activation by associating with p50 to suppress socs1.	the h3k4me3 demethylase kdm5a regulates gene transcription and is implicated in carcinogenesis. however, the role of kdm5a in innate immune response remains poorly understood. here, we demonstrate that kdm5a deficiency impairs activation of natural killer (nk) cells, with decreased ifn-γ production. accordingly, kdm5a(-/-) mice are highly susceptible to listeria monocytogenes (lm) infection. during nk cell activation, loss of kdm5a profoundly impairs phosphorylation and nuclear localization of s ...	2016	27050510
optimizing application parameters for lactic acid and sodium metasilicate against pathogens on fresh beef, pork and deli meats.	lactic acid (la) and sodium metasilicate (sm) have been approved for use as antimicrobials on meat. the objectives were to determine optimum concentrations, temperatures and hot-water dips of la and sm for reduction of escherichia coli o157:h7, non-o157 shiga-toxin producing e. coli (stec), salmonella spp., and listeria monocytogenes on beef, pork and deli meats. la was applied at 1, 2, 3, and 4% and sm was applied at 2, 3, 4, and 5%. sm4 and la4 were the lowest concentrations most effective aga ...	2016	27050408
arginine patch predicts the rna annealing activity of hfq from gram-negative and gram-positive bacteria.	the sm-protein hfq facilitates interactions between small non-coding rna (srna) and target mrnas. in enteric gram-negative bacteria, hfq is required for srna regulation, and hfq deletion results in stress intolerance and reduced virulence. by contrast, the role of hfq in gram-positive is less established and varies among species. the rna binding and rna annealing activity of hfq from escherichia coli, pseudomonas aeruginosa, listeria monocytogenes, bacillus subtilis, and staphylococcus aureus we ...	2016	27049793
spovg is a conserved rna-binding protein that regulates listeria monocytogenes lysozyme resistance, virulence, and swarming motility.	in this study, we sought to characterize the targets of the abundantlisteria monocytogenesnoncoding rna rli31, which is required forl. monocytogeneslysozyme resistance and pathogenesis. whole-genome sequencing of lysozyme-resistant suppressor strains identified loss-of-expression mutations in the promoter ofspovg, and deletion ofspovgrescued lysozyme sensitivity and attenuationin vivoof therli31mutant. spovg was demonstrated to be an rna-binding protein that interacted with rli31in vitro.the rel ...	2016	27048798
nanoemulsions of thymol and eugenol co-emulsified by lauric arginate and lecithin.	lauric arginate (lae) is a cationic surfactant with excellent antimicrobial activities. to incorporate essential oil components (eocs) in aqueous systems, properties of eoc nanoemulsions prepared with a lae and lecithin mixture were studied. the lae-lecithin mixture resulted in stable translucent nanoemulsions of thymol and eugenol with spherical droplets smaller than 100nm, contrasting with the turbid emulsions prepared with individual emulsifiers. zeta-potential data suggested the formation of ...	2016	27041312
evidence of metabolically active but non-culturable listeria monocytogenes in long-term growth at 10 °c.	cultures of listeria monocytogenes at low temperatures (10 °c) in a broth model revealed long-term survival at about 0.04% cell density in relation to the log phase. in contrast, direct viable counts and pma real-time pcr data suggested that 50% and 1% of the population retain membrane integrity, respectively. to elucidate the observed difference, the metabolic activity of the bacterial population was investigated by multiparametric flow cytometry, including the assessment of membrane integrity, ...	2016	27040843
evaluation of a cross contamination model describing transfer of salmonella spp. and listeria monocytogenes during grinding of pork and beef.	in a previous study, a model was developed to describe the transfer and survival of salmonella during grinding of pork (møller, c.o.a., nauta, m.j., christensen, b.b., dalgaard, p., hansen, t.b., 2012. modelling transfer of salmonella typhimurium dt104 during simulation of grinding of pork. journal of applied microbiology 112 (1), 90-98). the robustness of this model is now evaluated by studying its performance for predicting the transfer and survival of salmonella spp. and listeria monocytogene ...	2016	27035678
genome sequences of five nonvirulent listeria monocytogenes serovar 4 strains.	we present the complete genome sequences of five nonpathogeniclisteria monocytogenesserovar 4 strains: wslc 1018 (4e), 1019 (4c), 1020 (4a), 1033 (4d), and 1047 (4d). these sequences may help to uncover genes involved in the synthesis of the serovar antigens-phenotypic determinants of virulence deemed clinically relevant.	2016	27034489
mechanistic studies of the agmatine deiminase from listeria monocytogenes.	listeria monocytogenes is a gram-positive food-borne pathogen that is capable of living within extreme environments (i.e. low temperatures and ph). this ability to survive in such conditions may arise, at least in part, from agmatine catabolism via the agmatine deiminase system (agds). this catabolic pathway utilizes an agmatine deiminase (agd) to hydrolyse agmatine into n-carbamoylputrescine (ncp), with concomitant release of ammonia, which increases the ph, thus mitigating the ill effects of t ...	2016	27034081
the regulatory roles of ncrna rli60 in adaptability of listeria monocytogenes to environmental stress and biofilm formation.	listeria monocytogenes is a facultative anaerobic gram-positive bacterium. it is well adapted to external environments and able to infect both humans and animals. to understand the impacts of ncrna rli60 on the adaptability of l. monocytogenes to environmental stresses and biofilm formation, a rli60 deletion strain of l. monocytogenes (lm-δrli60) was constructed using splicing by overlap extension pcr (soe-pcr) and homologous recombination and then compared it with wild-type strain l. monocytoge ...	2016	27032404
retail deli slicer cleaning frequency--six selected sites, united states, 2012.	listeria monocytogenes (listeria) causes the third highest number of foodborne illness deaths (an estimated 255) in the united states annually, after nontyphoidal salmonella species and toxoplasma gondii (1). deli meats are a major source of listeriosis illnesses, and meats sliced and packaged at retail delis are the major source of listeriosis illnesses attributed to deli meat (4). mechanical slicers pose cross-contamination risks in delis and are an important source of listeria cross-contamina ...	2016	27031689
effect of fermented soybean products intake on the overall immune safety and function in mice.	various functional activities have been reported for the fermented soybean products doenjang (dj) and cheonggukjang (cgj), although no systemic investigations of their immune functions have been conducted to date. we examined the effects of an experimental diet of dj, cgj, or a mixture of unfermented raw material for 4 weeks on overall immunity and immune safety in mice. no significant alterations were observed in peripheral or splenic immune cells among groups. enhanced splenic natural killer c ...	2017	27030201
influence of sterilized human fecal extract on the sensitivity of salmonella enterica atcc 13076 and listeria monocytogenes atcc 15313 to enrofloxacin.	there is much debate on whether continuous exposure of commensal bacteria and potential pathogens residing in the human intestinal tract to low levels of antimicrobial agents from treated food animals pose a public health concern. to investigate antimicrobial effects on bacteria under colonic conditions, we studied resistance development in salmonella enterica and listeria monocytogenes exposed to enrofloxacin in the presence of fecal extract. the bacteria were incubated at 37 °c in mueller-hint ...	2013	27029316
efficacy of neutral electrolyzed water, quaternary ammonium and lactic acid-based solutions in controlling microbial contamination of food cutting boards using a manual spraying technique.	bactericidal activity of neutral electrolyzed water (new), quaternary ammonium (quat), and lactic acid-based solutions was investigated using a manual spraying technique against salmonella typhimurium, escherichia coli o157:h7, campylobacter jejuni, listeria monocytogenes and staphylococcus aureus that were inoculated onto the surface of scarred polypropylene and wooden food cutting boards. antimicrobial activity was also examined when using cutting boards in preparation of raw chopped beef, chi ...	2016	27027449
whole-genome sequencing used to investigate a nationwide outbreak of listeriosis caused by ready-to-eat delicatessen meat, denmark, 2014.	listeriosis is a serious foodborne infection. outbreaks of listeriosis occur rarely, but have often proved difficult to solve. in june 2014, we detected and investigated a listeriosis outbreak in denmark using patient interviews and whole-genome sequencing (wgs).	2016	27025820
spatial organization of cell wall-anchored proteins at the surface of gram-positive bacteria.	bacterial surface proteins constitute an amazing repertoire of molecules with important functions such as adherence, invasion, signalling and interaction with the host immune system or environment. in gram-positive bacteria, many surface proteins of the "lpxtg" family are anchored to the peptidoglycan (pg) by an enzyme named sortase. while this anchoring mechanism has been clearly deciphered, less is known about the spatial organization of cell wall-anchored proteins in the bacterial envelope. h ...	2016	27025379
a stable live bacterial vaccine.	formulating vaccines into a dry form enhances its thermal stability. this is critical to prevent administering damaged and ineffective vaccines, and to reduce its final cost. a number of vaccines in the market as well as those being evaluated in the clinical setting are in a dry solid state; yet none of these vaccines have achieved long-term stability at high temperatures. we used spray-drying to formulate a recombinant live attenuated listeria monocytogenes (lm; expressing francisella tularensi ...	2016	27020530
comparing listeriosis risks in at-risk populations using a user-friendly quantitative microbial risk assessment tool and epidemiological data.	although infection by the pathogenic bacterium listeria monocytogenes is relatively rare, consequences can be severe, with a high case-fatality rate in vulnerable populations. a quantitative, probabilistic risk assessment tool was developed to compare estimates of the number of invasive listeriosis cases in vulnerable canadian subpopulations given consumption of contaminated ready-to-eat delicatessen meats and hot dogs, under various user-defined scenarios. the model incorporates variability and ...	2016	27019157
overexpression of angiotensin-converting enzyme in myelomonocytic cells enhances the immune response.	angiotensin-converting enzyme (ace) converts angiotensin i to the vasoconstrictor angiotensin ii and thereby plays an important role in blood pressure control. however, ace is relatively non-specific in its substrate specificity and cleaves many other peptides. recent analysis of mice overexpressing ace in monocytes, macrophages, and other myelomonocytic cells shows that these animals have a marked increase in resistance to experimental melanoma and to infection by listeria monocytogenes or meth ...	2016	27018193
acidified nitrite inhibits proliferation of listeria monocytogenes - transcriptional analysis of a preservation method.	sodium nitrite (nano2) is added as a preservative during raw meat processing such as raw sausage production to inhibit growth of pathogenic bacteria. in the present study it was shown in challenge assays that the addition of sodium nitrite indeed inhibited growth and survival of listeria monocytogenes in short-ripened spreadable raw sausages. furthermore, in vitro growth analyses were performed, which took into account combinations of various parameters of the raw sausage ripening process like t ...	2016	27017279
antimicrobial properties of microemulsions formulated with essential oils, soybean oil, and tween 80.	it was previously found that blending soybean oil with cinnamon bark oil (cbo), eugenol or thyme oil, tween 80, and equal masses of water and propylene glycol could be used to prepare microemulsions. in the present study, the objective was to determine the antimicrobial activity of the microemulsions in tryptic soy broth (tsb) and 2% reduced fat milk. in tsb, the minimum inhibitory concentration (mic) of cbo solubilized in microemulsions was up to 625 ppm against cocktails of listeria monocytoge ...	2016	27016636
unexpected positive control of nfκb and mir-155 by dgkα and ζ ensures effector and memory cd8+ t cell differentiation.	signals from the t-cell receptor (tcr) and γ-chain cytokine receptors play crucial roles in initiating activation and effector/memory differentiation of cd8 t-cells. we report here that simultaneous deletion of both diacylglycerol kinase (dgk) α and ζ (dko) severely impaired expansion of cd8 effector t cells and formation of memory cd8 t-cells after listeria monocytogenes infection. moreover, ablation of both dgkα and ζ in preformed memory cd8 t-cells triggered death and impaired homeostatic pro ...	0	27014906
silver as antibacterial toward listeria monocytogenes.	listeria monocytogenes is a serious foodborne pathogen that can contaminate food during processing and can grow during food shelf-life. new types of safe and effective food contact materials embedding antimicrobial agents, like silver, can play an important role in the food industry. the present work aimed at evaluating the in vitro growth kinetics of different strains of l. monocytogenes in the presence of silver, both in its ionic and nano form. the antimicrobial effect was determined by assay ...	2016	27014230
acidic electrolyzed water as a novel transmitting medium for high hydrostatic pressure reduction of bacterial loads on shelled fresh shrimp.	acidic electrolyzed water (aew), a novel non-thermal sterilization technology, is widely used in the food industry. in this study, we firstly investigated the effect of aew as a new pressure transmitting medium for high hydrostatic pressure (aew-hhp) processing on microorganisms inactivation on shelled fresh shrimp. the optimal conditions of aew-hhp for vibrio parahaemolyticus inactivation on sterile shelled fresh shrimp were obtained using response surface methodology: nacl concentration to ele ...	2016	27014228
characterization of antimicrobial activity against listeria and cytotoxicity of native melittin and its mutant variants.	antimicrobial peptides (amps) are relatively short peptides that have the ability to penetrate the cell membrane, form pores leading to cell death. this study compares both antimicrobial activity and cytotoxicity of native melittin and its two mutants, namely, melittin i17k (gigavlkvlttglpalkswikrkrqq) with a higher charge and lower hydrophobicity and mutant g1i (iigavlkvlttglpaliswikrkrqq) of higher hydrophobicity. the antimicrobial activity against different strains of listeria was investigate ...	2016	27011349
a structural comparison of listeria monocytogenes protein chaperones prsa1 and prsa2 reveals molecular features required for virulence.	listeria monocytogenes is a gram-positive environmental bacterium that lives within soil but transitions into a pathogen upon contact with a mammalian host. the transition of l. monocytogenes from soil dweller to cytosolic pathogen is dependent upon secreted virulence factors that mediate cell invasion and intracellular growth. prsa1 and prsa2 are secreted bacterial lipoprotein chaperones that contribute to the folding of proteins translocated across the bacterial membrane; prsa2 is required for ...	2016	27007641
development of new 5-(chromene-3-yl)methylene-2,4-thiazolidinediones as antimicrobial agents.	in the context of the increasing phenomenon of microbial resistance to usual drugs, the development of new treatment strategies and new therapeutic protocols is a constant need. thiazolidinedione and chromone represent two important scaffolds in medicinal chemistry due to their large pharmacological applicability.	2016	27004035
effect of iranian ziziphus honey on growth of some foodborne pathogens.	honey has previously been shown to have wound healing and antimicrobial properties, but this is dependent on the type of honey, geographical location, and flower from which the final product is derived. we tested the antimicrobial activity of a natural honey originating from the ziziphus spina-christi tree, against selected strains of bacteria. ziziphus honey among more than a 100 verities of honey is known to have the greatest value of energy and minerals in it.	2017	27003970
eugenol in combination with lactic acid bacteria attenuates listeria monocytogenes virulence in vitro and in invertebrate model galleria mellonella.	listeria monocytogenes is a human enteric pathogen that causes severe foodborne illness in high-risk populations. crossing the intestinal barrier is the first critical step for listeria monocytogenes infection. therefore, reducing l. monocytogenes colonization and invasion of intestinal epithelium and production of virulence factors could potentially control listeriosis in humans. this study investigated the efficacy of sub-inhibitory concentration (sic) of the plant-derived antimicrobial eugeno ...	2016	27002648
structural basis of rifampin inactivation by rifampin phosphotransferase.	rifampin (rif) is a first-line drug used for the treatment of tuberculosis and other bacterial infections. various rif resistance mechanisms have been reported, and recently an rif-inactivation enzyme, rif phosphotransferase (rph), was reported to phosphorylate rif at its c21 hydroxyl at the cost of atp. however, the underlying molecular mechanism remained unknown. here, we solve the structures of rph from listeria monocytogenes (lmrph) in different conformations. lmrph comprises three domains: ...	2016	27001859