Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| agrobacterium-mediated transformation of quaking aspen (populus tremuloides) and regeneration of transgenic plants. | agrobacterium-mediated gene transformation of populus tremuloides michx was accomplished by co-cultivation of leaf disks excised from greenhouse plants with agrobacterium tumefaciens containing a binary ti-plasmid vector harboring chimeric neomycin phosphotransferase (npt ii) and ß-glucuronidase (gus) genes. shoot regeneration in the presence of kanamycin was achieved when thidiazuron (tdz) was used as a plant growth regulator. transformation was verified by amplification of npt ii and gus gene ... | 1994 | 24192872 |
| transformation of sunflower (helianthus annuus l.): a reliable protocol. | a reliable protocol for the transformation of cultivated sunflower (helianthus annuus l.) has been established, based on microprojectile bombardment of half shoot apices in combination with agrobacterium tumefaciens coculture. transgenic shoots have been obtained from 5 inbred lines, although transformation efficiencies varied with the genotype. plants expressing the transgenes could be recovered from up to 7% of the explants. a minority of plants was shown to be chimaeric for expression of ß-gl ... | 1994 | 24192870 |
| construction of a designer chromosome in tobacco. | the tobacco (nicotiana tabacum l.) breeding line nc 152 is a doubled haploid that possesses an addition chromosome from n. africana [merxm. and buttler]. a gene on this chromosome confers potyvirus resistance (poty(r)). our objective was to use the addition chromosome as a base on which to construct a designer chromosome containing a foreign gene linkage package. a mutant dhfr gene conferring resistance to methotrexate (mtx) was inserted into nc 152-haploid (n = 25) leaf tissue via agrobacterium ... | 1994 | 24190470 |
| virulence of different agrobacterium strains on hairy root formation of hyoscyamus muticus. | hyoscyamus muticus accession was evaluated for its response to inoculation with agrobacterium rhizogenes strains lba9402, a4, 15834, and agrobacterium tumefaciens strain c58 ci prt gus104. the agrobacterium strain used for the transformation has a significant influence on the phenotype of the clone as well as on the growth rate and hyoscyamine production of these root culture clones. the most virulent strains were c58 ci prt gus104 and lba9402. more roots were obtained on lso medium than on b50 ... | 1995 | 24190302 |
| transformation of sunflower (helianthus annuus l.) following wounding with glass beads. | a procedure was developed for transformation of helianthus annuus (sunflower) using agrobacterium tumefaciens. cotyledons were removed from young seedlings, and the remaining tissue was uniformly wounded by shaking with glass beads. the wounded tissue was then co-cultivated with a hypervirulent strain of agrobacterium tumefaciens harboring the binary plasmid pcnl56. minimal use of defined medium was required, and no callus was observed. the polymerase chain reaction (pcr) followed by dna hybridi ... | 1995 | 24186761 |
| agrobacterium-mediated transformation of almond leaf pieces. | a protocol for agrobacterium-mediated transformation of leaf pieces from two almond cultivars has been developed. this protocol gave a transformation percentage of 48.6 for the cultivar mn51 and 27.9 for the cultivar 'supernova'. the agrobacterium tumefaciens strain lba 4404 carrying a binary vector plasmid pbingusint has been used. a critical step for optimal transformation was the pre-culture period of leaf pieces prior selection on medium containing kanamycin. both cultivars showed an almost ... | 1995 | 24186758 |
| biosynthesis, processing and targeting of the g-protein of vesicular stomatitis virus in tobacco protoplasts. | leaf protoplasts of tobacco (nicotlana tabacum l.) were employed for transfection of chimeric transcriptional gene fusions comprising the 35s promoter from cauliflower mosaic virus, the coding sequence of the g-protein from vesicular stomatitis virus (vsvg) and the transcriptional terminator from the agrobacterium tumefaciens nopaline-synthetase gene. transient expression of the chimeric gene was monitored through northern analysis of total protoplast rna using a labeled vsv cdna probe, and thro ... | 1992 | 24186728 |
| agrobacterium-mediated transformation of peanut (arachis hypogaea l.) embryo axes and the development of transgenic plants. | transgenic peanut (arachis hypogaea l.) plants have been produced using an agrobacterium-mediated transformation system. zygotic embryo axes from mature seed were cocultured with agrobacterium tumefaciens strain eha101 harboring a binary vector that contained the genes for the scorable marker b-glucuronidase (gus) and the selectable marker neomycin phosphotransferase ii. nine percent of the germinated seedlings were gus+. polymerase chain reaction analysis confirmed that gus+ shoots and t1 proge ... | 1995 | 24186625 |
| expression of a wild-type gbss gene introduced into an amylose-free potato mutant by agrobacterium tumefaciens and the inheritance of the inserts at the microsporic level. | granule-bound starch synthase (gbss) catalyses the synthesis of amylose in starch granules. transformation of a diploid amylose-free (amf) potato mutant with the gene encoding gbss leads to the restoration of amylose synthesis. transformants were obtained which had wild-type levels of both gbss activity and amylose content. it proved to be difficult to increase the amylose content above that of the wild-type potato by the introduction of additional copies of the wild-type gbss gene. staining of ... | 1994 | 24186021 |
| modification of competence for in vitro response to fusarium oxysporum in tomato cells. ii. effect of the integration of agrobacterium tumefaciens genes for auxin and cytokinin synthesis. | we have studied the effect of a change in the endogenous hormone equilibria on the competence of tomato (lycopersicon esculentum) cells to defend themselves against the fungal pathogen fusarium oxysporum f. sp. lycopersici. calluses from cvs 'davis' and 'red river', respectively resistant and susceptible to fusarium and transgenic for an auxin- or cytokinin-synthesizing gene from agrobacterium tumefaciens, were used. the integration of agrobacterium hormone-related genes into susceptible cv 'red ... | 1994 | 24185887 |
| agrobacterium tumefaciens-mediated transformation of broccoli (brassica oleracea var. italica) and cabbage (b. oleracea var. capitata). | transgenic broccoli (brassica oleracea var. italica) was produced by two agrobacterium tumefaciens-mediated transformation methods. one used flowering stalk explants from mature plants; the other used hypocotyl and petiole explants from in vitro-grown seedlings. several hundred transformants containing a bacillus thuringiensis ∂-endotoxin gene (cryia(c)-type) and the neomycin phosphotransferase gene were recovered. rooted transformants were obtained in as little as 3 months using seedling explan ... | 1995 | 24185794 |
| transformation of peas (pisum sativum l.) using immature cotyledons. | a reliable agrobacterium tumefaciens-mediated transformation method has been developed for peas (pisum sativum) using immature cotyledons as the explant source. transgenic plants were recovered from the four cultivars tested: bolero, trounce, bohatyr and huka. the method takes approximately 7 months from explant to seed-bearing primary regenerant. the binary vector used carried genes for kanamycin and phosphinothricin resistance. transformed pea plants were selected on 10 mg/l phosphinothricin. ... | 1995 | 24185786 |
| transformation of solanum brevidens using agrobacterium tumefaciens. | leaf pieces of in vitro-cultured plantlets of the wild potato species solanum brevidens phil. were cocultivated with agrobacterium tumefaciens that contained nptii and uida genes on the disarmed plasmid pbi121. independent transgenic shoots were regenerated from solidified and liquid medium that contained 50 mg l(-1) kanamycin. two agrobacterium strains were investigated for transformation efficiency. gv2260, which contained p35sgusint, resulted in a 11% transformation frequency, compared with 1 ... | 1995 | 24185775 |
| genetic transformation ofpelargonium x hortorum. | transgenicpelargonium x hortorum have been producedvia agrobacterium tumefaciens-mediated transformation. the regeneration protocol used provided a regeneration frequency approximately to 95 percent. clumps of regenerants, from cotyledons and hypocotyls ofpelargonium x hortorum seedlings, were inoculated with the disarmed strain eha101 ofagrobacterium tumefaciens. this strain contains a binary vector carrying neomycin phosphotransferase ii, hygromycin b phosphotransferase and ß-glucuronidase gen ... | 1995 | 24185656 |
| transformation of grape (vitis vinifera l.) zygotic-derived somatic embryos and regeneration of transgenic plants. | transgenic grape plants were regenerated from somatic embryos derived from immature zygotic embryos of seedless grape (vitis vinifera l.) selections. somatic embryos were bombarded twice with 1 μm gold particles using the biolistic pds-1000/he device (bio-rad laboratories) and then exposed to agrobacterium tumefaciens strain c58/z707 containing the binary plasmid pga482gg or pcgn7314. following cocultivation, secondary embryos were allowed to proliferate on emershad/ramming proliferation (erp) m ... | 1995 | 24185603 |
| transformation of grapevine rootstocks with the coat protein gene of grapevine fanleaf nepovirus. | control of fanleaf disease induced by the grapevine fanleaf nepovirus (gflv) today is based on sanitary selection and soil disinfection with nematicides. this way of control is not always efficient and nematicides can be dangerous pollutants. coat protein (cp) mediated protection could be an attractive alternative. we have transferred a chimeric cp gene of gflv-f13 via agrobacterium tumefaciens lba4404 into two rootstock varieties: vitis rupestris and 110 richter (v. rupestris x v. berlandieri). ... | 1995 | 24185595 |
| efficient genetic transformation of red raspberry, rubus ideaus l. | we have developed an efficient transformation system for red raspberry (rubus ideaus l.) using agrobacterium mediated gene transfer. using this system we have successfully introduced a gene that encodes an enzyme, s-adenosylmethionine hydrolase (samase), in raspberry cultivars meeker (mk), chilliwack (ch) and canby (cy). leaf and petiole expiants were inoculated with disarmed agrobacterium tumefaciens strain eha 105 carrying either of two binary vectors, pag1452 or pag1552, encoding gene sequenc ... | 1995 | 24185514 |
| regeneration of transgenic plants from the commercial apple cultivar royal gala. | a transformation system was developed for the commercial apple (malus x domestica borkh.) cultivar royal gala. leaf pieces from in vitro-grown shoots were cocultivated for 2 days with agrobacterium tumefaciens strain lba4404 containing the binary vectors pkiwi105 or pkiwi110. shoots were produced on a shooting medium containing kanamycin (50 mg·l(-1)). a 2-day incubation period on kanamycin-free medium prior to antibiotic selection enhanced the regeneration of kanamycin-resistant shoots. the maj ... | 1995 | 24185446 |
| forskolin synthesis in in vitro cultures of coleus forskohlii briq transformed with agrobacterium tumefaciens. | agrobacterium tumefaciens mediated tumor tissue and shooty teratomas of coleus forskohlii were cultured in vitro. forskolin was detected in tumorous callus (0.002%), rhizogenic callus (0.011%) and root cultures (0.014%), but not in shooty teratomas. forskolin synthesis and accumulation in tumorous c. forskohlii cultures may permit the elucidation of diterpene metabolism in this species. | 1996 | 24178612 |
| production of fertile transgenic peanut (arachis hypogaea l.) plants using agrobacterium tumefaciens. | fertile transgenic plants of peanut (arachis hypogaea l. cv. new mexico valencia a) were produced using an agrobacterium-mediated transformation system. leaf section explants were inoculated with a. tumefaciens strain eha105 harboring the binary vector pbi121 containing the genes for β-glucuronidase (gus) and neomycin phosphotransferase ii (nptii). approximately 10% of the shoots regenerated on selection medium were gus-positive. five independent transformation events resulted in the production ... | 1996 | 24178604 |
| shoot regeneration and agrobacterium-mediated transformation of fragaria vesca l. | an efficient and reliable method for shoot regeneration from leaf disks of fragaria vesca l. has been developed. this protocol has been successfully employed to obtain transformed plants using agrobacterium tumefaciens as gene vector. murashige and skoog basal medium supplemented with benzyladenine (4 mg/l) and indole-3-butyric acid (0.25 mg/l) induced the maximum percentage of shoot regeneration (98%) and the highest number of shoot colonies per explant (4.6) after 8 weeks of culture. isolated ... | 1996 | 24178534 |
| genetic transformation of begonia tuberhybrida by ri rol genes. | we have developed an agrobacterium -mediated transformation system for commercial begonia species. the leaf explants of begonia semperflorens, begonia x hiemalis and b. tuberhybrida were inoculated with agrobacterium tumefaciens lba4404 harboring a binary vector pbi121 which contains rola, b and c genes of an agropine type ri plasmid (pria4b). kanamycin resistant shoots of b. tuberhybrida were obtained on ms agar medium supplemented with 0.1 mg/l naa, 0.5 mg/l ba, 500 mg/l claforan and 100 mg/l ... | 1996 | 24178527 |
| transformation of pickling cucumber with chitinase-encoding genes using agrobacterium tumefaciens. | transformation of cucumber cv. endeavor was attempted using three agrobacterium tumefaciens strains (a supervirulent leucinopine type, an octopine type and a nopaline type), each harbouring one of three binary vectors which contained an acidic chitinase gene from petunia, and basic chitinase genes from tobacco and bean, respectively, driven by the camv 35s promoter. petiole explants were inoculated with a bacterial suspension (10(8) cells·ml(-1)), cocultivated for 48-96 h and placed on murashige ... | 1996 | 24178524 |
| agrobacterium tumefaciens-mediated transformation of solanum gilo raddi as influenced by explant type. | an efficient system for agrobacterium tumefaciens-mediated transformation of solanum gilo was established. the marker genes for kanamycin resistance and ß-glucuronidase expression were introduced. a comparison between cotyledon and hypocotyl explants showed that while regeneration was better from hypocotyl explants, cotyledon explants gave better transformation efficiency (46% vs. 32%). four levels of kanamycin selection (100, 150, 200 and 250 mg/l) were tested for effect on transformation effic ... | 1996 | 24178522 |
| improvements in the transformation of arabidopsis thaliana c24 leaf-discs by agrobacterium tumefaciens. | we report here an efficient arabidopsis leafdisc transformation protocol yielding an average transformation frequency of 1.6 transgenic shoots per leaf explant 4 weeks after the bacterial infection period. subsequent cultivation in vitro is such that a high percentage (85-90%) of the primary transformants produces seeds with an average seed yield of 100-300 seeds per plant. this improved transformation protocol yields mainly (70%) transformants segregating for a single t-dna locus of which 68% a ... | 1996 | 24178520 |
| agrobacterium-mediated transformation of apple (malus x domestica borkh.): an assessment of factors affecting regeneration of transgenic plants. | we have previously developed a protocol for efficient gene transfer and regeneration of transgenic calli following cocultivation of apple (cv. jonagold) explants with agrobacterium tumefaciens (de bondt et al. 1994, plant cell reports 13: 587-593). now we report on the optimization of postcultivation conditions for efficient and reproducible regeneration of transgenic shoots from the apple cultivar jonagold. factors which were found to be essential for efficient shoot regeneration were the use o ... | 1996 | 24178471 |
| agrobacterium t-dna mutation causes the loss of gus expression in transgenic tobacco. | a new freezer stock of an agrobacterium tumefaciens clone of lba4404/pbi121, designated 8999, was found to contain a mutation in the t-dna region. gus activity in agrobacterium 8999 was reduced to levels in negative controls of lba4404. additionally, gus activity in t1 seedlings from tobacco plants transformed with 8999 was reduced to that of untransformed plants. southern and northern blotting showed that agrobacterium clone 8999 transferred its t-dna into the plant, that the correct sizes of 3 ... | 1996 | 24178420 |
| agrobacterium tumefaciens-mediated transformation of vigna mungo (l.) hepper. | transformed vigna mungo (blackgram) calli were obtained by cocultivating segments of primary leaves with agrobacterium tumefaciens vir helper strains harbouring the binary vector pga472 having kanamycin resistance gene as plant transformation marker. transformed calli were selected on murashige and skoog medium supplemented with 50 mg/l kanamycin and 500 mg/l carbenicillin. transformed calli were found to be resistant to kanamycin up to 900 mg/l concentration. expression of kanamycin resistance ... | 1996 | 24178351 |
| transformation of barrel medic (medicago truncatula gaertn.) by agrobacterium tumefaciens and regeneration via somatic embryogenesis of transgenic plants with the mtenod12 nodulin promoter fused to the gus reporter gene. | fertile and stable transgenic plants of the model legume medicago truncatula gaertn. were obtained through transformation of leaf tissue with the disarmed agrobacterium tumefaciens strain lba4404 and in vitro regeneration via somatic embryogenesis. an optimised transformation/regeneration protocol has been established for two genotypes of the cultivar jemalong, including a previously described highly embryogenic line (nolan et al. 1989, plant cell rep. 8: 278-281). using this protocol, transgeni ... | 1996 | 24178347 |
| genetic transformation of cotyledon explants of cowpea (vigna unguiculata l. walp) using agrobacterium tumefaciens. | mature de-embryonated cotyledons with intact proximal end of vigna unguiculata were cultured on b5 basal medium containing varying concentrations of bap. thirty-six percent of the explants produced shoots on b5 medium supplemented with 8× 10(-6) m bap. cotyledon explants were pre-incubated for 24 h, inoculated with a. tumefaciens pucd2614 carrying pucd2340, co-cultivated for 48 h and transferred to hygromycin-b (25 mg/l) containing shoot induction medium. approximately 15-19% of the explants pro ... | 1996 | 24178287 |
| agrobacterium tumefaciens-mediated transformation of artemisia annua l. and regeneration of transgenic plants. | a transformation system was developed for artemisia annua l. plants. leaf explants from in vitro grown plants developed callus and shoots on medium with 0.05 mg/l naphthaleneacetic acid and 0.5 mg/l n(6)-benzyladenine after transformation with the c58c1 rif(r) (pgv2260) (ptjk136) agrobacterium tumefaciens strain. a concentration of 20 mg/l kanamycin was added in order to select transformed tissue. kanamycin resistant shoots were rooted on naphthaleneacetic acid 0.1 mg/l. polymerase chain reactio ... | 1996 | 24178277 |
| transformation of cucumber (cucumis sativus l.) plants using agrobacterium tumefaciens and regeneration from hypocotyl explants. | transgenic cucumber (cucumis sativus l.) plants were successfully obtained from hypocotyl explants inoculated with agrobacterium tumefaciens, which harbored a binary vector plasmid with nos-nptii, camv 35s-i-gus and camv 35s-hph genes. acetosyringone enhanced the efficiency of transformation at the cut surface cells of hypocotyl explants during five days of co-cultivation. transformed cells were more effectively selected using 20-30 mg/l hygromycin b than using 50-100 mg/l kanamycin. shoot regen ... | 1996 | 24178213 |
| transgenic plant production from leaf discs of moricandia arvensis using agrobacterium tumefaciens. | a high frequency shoot regeneration (80%) was developed from callus of leaf discs and stem internodes of moricandia arvensis. leaf discs were shown to be a preferable starting material for transformation experiments. agrobacterium tumefaciens strain gv3101/pmp90 used in this study contained a binary vector with genes for kanamycin resistance, hygromycin resistance and β-glucuronidase (gus). maximum transformation efficiency (10.3%) was achieved by using kanamycin at the rate of 200 mg/l as a sel ... | 1996 | 24178211 |
| auxin autonomy in cultured tobacco teratoma tissues transformed by an auxin-mutant strain of agrobacterium tumefaciens. | we have studied the mechanism of auxin autonomy in tobacco (nicotiana tabacum l.) crowngall tissues transformed by the auxin-mutant (tms (-)) a66 strain of agrobacterium tumefaciens. normally, tms (-) tobacco tumor tissues require the formation of shoots to exhibit auxin-independent growth in culture. we have isolated from tms (-) tobacco cells several stable variants that are fully hormone-independent and grow rapidly as friable, unorganized tissues, thus mimicking the growth and morphology of ... | 1992 | 24178208 |
| transgenic plant production mediated by agrobacterium in indica rice. | a reproducible system has been developed for the production of transgenic plants in indica rice using agrobacterium-mediated gene transfer. three-week-old scutella calli served as an excellent starting material. these were infected with an agrobacterium tumefaciens strain eha101 carrying a plasmid pig121hm containing genes for β-glucuronidase (gus) and hygromycin resistnace (hygr). hygromycin (50 mg/l) was used as a selectable agent. inclusion of acetosyringone (50μm) in the agrobacterium suspen ... | 1996 | 24178159 |
| expression of soybean-embryo lipoxygenase 2 in transgenic tobacco tissue. | to assess the role of lipoxygenase (lox; ec 1.13.11.12) in plants, we increased the expression of lox in the tissues of nicotiana tabacum l. cv. 'ky 14' by over-expression of the lox2 gene from the soybean (glycine max (l.) merrill) embryo. the lox2 cdna was manipulated by replacing its 5'-untranslated sequence with the translational enhancer of the alfalfa mosaic virus (amv), and subcloned into a plant expression vector, 3' to a duplicated cauliflower mosaic virus 35s promoter. the amv-lox2 con ... | 1992 | 24178044 |
| genetic transformation of major cereal crops. | of the more than 50,000 edible plant species in the world, at least 10,000 species are cereal grains. three major cereal crops, rice (oryza sativa), maize (zea mays), and wheat (triticum sp.), provide two-thirds of the world's food energy intake. although crop yields have improved tremendously thanks to technological advances in the past 50 years, population increases and climate changes continue to threaten the sustainability of current crop productions. whereas conventional and marker-assisted ... | 2013 | 24166432 |
| higher plant transformation: principles and molecular tools. | in higher plants, genetic transformation, which is part of the toolbox for the study of living organisms, had been reported only 30 years ago, boosting basic plant biology research, generating superior crops, and leading to the new discipline of plant biotechnology. here, we review its principles and the corresponding molecular tools. in vitro regeneration, through somatic embryogenesis or organogenesis, is discussed because they are prerequisites for the subsequent agrobacterium tumefaciens-med ... | 2013 | 24166431 |
| the roles of bacterial and host plant factors in agrobacterium-mediated genetic transformation. | the genetic transformation of plants mediated by agrobacterium tumefaciens represents an essential tool for both fundamental and applied research in plant biology. for a successful infection, culminating in the integration of its transferred dna (t-dna) into the host genome, agrobacterium relies on multiple interactions with host-plant factors. extensive studies have unraveled many of such interactions at all major steps of the infection process: activation of the bacterial virulence genes, cell ... | 2013 | 24166430 |
| from the tumor-inducing principle to plant biotechnology and its importance for society. | this dialogue was held between the guest editors of the special issue on "plant transgenesis" of the int. j. dev. biol. and marc van montagu. research in the group of marc van montagu and jeff schell in the 1970s was essential to reveal how the phytopathogenic bacterium agrobacterium tumefaciens transfers dna to host plants to cause crown gall disease. knowledge of the molecular mechanism underlying gene transfer, subsequently led to the development of plant transgene technology, an indispensabl ... | 2013 | 24166428 |
| fundamental discoveries and simple recombination between circular plasmid dnas led to widespread use of agrobacterium tumefaciens as a generalized vector for plant genetic engineering. | fundamental research aimed to determine the limits of the agrobacterium transfer dna (t-dna) element that stably inserted into plant nuclear dna to cause crown gall tumor formation. the t-dna borders were discovered to be exceedingly precise, revealing that t-dna insertion into the plant genome was reproducible and exact. deletion of the internal regions of the t-dna, to remove the tumor forming genes, while retaining the t-dna borders, resulted again in efficient dna transfer to plant cells, bu ... | 2013 | 24166427 |
| thirty years of transgenic research in plants. | in 1983, the first transgenic tissues and plants were generated by means of disarmed agrobacterium tumefaciens strains, in which the oncogenes had been replaced by antibiotic resistance markers. hence, this special issue of the international journal of developmental biology celebrates 30 years of transgenic research in plants! eminent scientists working in the field of plant transformation or plant biotechnology have contributed to this publication and reviewed the state of the art of their part ... | 2013 | 24166426 |
| targeted gene deletion in aspergillus fumigatus using microbial machinery and a recyclable marker. | the emerging invasive fungal pathogen aspergillus fumigatus causes very serious infections among immunocompromised patient populations. while the genome of this pathogen has been sequenced, a major barrier to better understanding the complex biology of this eukaryotic organism is a lack of tools for efficient genetic manipulation. to improve upon this, we have generated a new gene deletion system for a. fumigatus using yeast recombinational cloning and agrobacterium tumefaciens mediated transfor ... | 2013 | 24161898 |
| development of transgenic sweet potato with multiple virus resistance in south africa (sa). | multiple infections of sweet potato feathery mottle virus (spfmv), sweet potato chlorotic stunt virus (spcsv), sweet potato virus g (spvg) and sweet potato mild mottle virus (spmmv) cause a devastating synergistic disease complex of sweet potato (ipomoea batatas lam.) in kwazulu-natal, south africa. in order to address the problem of multiple virus infections and synergism, this study aimed to develop transgenic sweet potato (cv. blesbok) plants with broad virus resistance. coat protein gene seg ... | 2014 | 24158330 |
| agrobacterium tumefaciens-mediated transgenic plant and somaclone production through direct and indirect regeneration from leaves in stevia rebaudiana with their glycoside profile. | agrobacterium tumefaciens (eha-105 harboring pcambia 1304)-mediated transgenic plant production via direct regeneration from leaf and elite somaclones generation through indirect regeneration in stevia rebaudiana is reported. optimum direct regeneration frequency along with highest transformation frequency was found on ms + 1 mg/l bap + 1 mg/l naa, while indirect regeneration from callus was obtained on ms + 1 mg/l bap + 2 mg/l naa. successful transfer of gus-positive (gus assay and pcr-based co ... | 2014 | 24154495 |
| production of human tissue plasminogen activator (tpa) in cucumis sativus. | tissue plasminogen activator (tpa) as a serine protease with 72 kd molecular mass and 527 amino acids plays an important role in the fibrinolytic system and the dissolution of fibrin clots in human body. the collective production of this drug in plants such as cucumber, one of the most important vegetables in the world, could reduce its production costs. in this study, after scrutiny of the appropriate regeneration of cucumber plant (isfahan variety) on ms medium with naphthalene acetic acid hor ... | 2014 | 24152103 |
| generating phenotypic diversity in a fungal biocatalyst to investigate alcohol stress tolerance encountered during microbial cellulosic biofuel production. | consolidated bioprocessing (cbp) of lignocellulosic biomass offers an alternative route to renewable energy. the crop pathogen fusarium oxysporum is a promising fungal biocatalyst because of its broad host range and innate ability to co-saccharify and ferment lignocellulose to bioethanol. a major challenge for cellulolytic cbp-enabling microbes is alcohol inhibition. this research tested the hypothesis that agrobacterium tumefaciens--mediated transformation (atmt) could be exploited as a tool to ... | 2013 | 24147009 |
| spectroscopic and mechanistic studies of dinuclear metallohydrolases and their biomimetic complexes. | an enhanced understanding of the metal ion binding and active site structural features of phosphoesterases such as the glycerophosphodiesterase from enterobacter aerogenes (gpdq), and the organophosphate degrading agent from agrobacterium radiobacter (opda) have important consequences for potential applications. coupled with investigations of the metalloenzymes, programs of study to synthesise and characterise model complexes based on these metalloenzymes can add to our understanding of structur ... | 2014 | 24135968 |
| insertional mutagenesis and cloning of the gene required for the biosynthesis of the non-host-specific toxin in cochliobolus lunatus that causes maize leaf spot. | the maize pathotype cochliobolus lunatus causes curvularia leaf spot by producing a non-host-specific toxin known as methyl 5-(hydroxymethyl) furan-2-carboxylate (m5hf2c). however, related research that explores the genes that control the production of this toxin is rare. in the current work, agrobacterium tumefaciens-mediated transformation (atmt) was employed to tag the gene required for the biosynthesis of the toxin. of the 3,000 atmt transformants recovered, 4 showed a significant decline in ... | 2014 | 24134718 |
| targeting fungal genes by diced sirnas: a rapid tool to decipher gene function in aspergillus nidulans. | gene silencing triggered by chemically synthesized small interfering rnas (sirnas) has become a powerful tool for deciphering gene function in many eukaryotes. however, prediction and validation of a single sirna duplex specific to a target gene is often ineffective. rna interference (rnai) with synthetic sirna suffers from lower silencing efficacy, off-target effects and is cost-intensive, especially for functional genomic studies. with the explosion of fungal genomic information, there is an i ... | 2013 | 24130711 |
| high throughput agrobacterium tumefaciens-mediated germline transformation of mechanically isolated meristem explants of cotton (gossypium hirsutum l.). | agrobacterium tumefaciens mediates high frequency of germline transformation of cotton meristem explants. the meristem transformation system we developed is rapid, high throughput and genotype-flexible. we have developed a high throughput cotton transformation system based on direct agrobacterium inoculation of mechanically isolated meristem explants of cotton (gossypium hirsutum l.). the explants were inoculated with a disarmed a. tumefaciens strain, ab33 harboring a 2 t-dna binary vector pmon1 ... | 2014 | 24129847 |
| agrobacterium t-dna-encoded protein atu6002 interferes with the host auxin response. | several genes in the agrobacterium tumefaciens transferred (t)-dna encode proteins that are involved in developmental alterations, leading to the formation of tumours in infected plants. we investigated the role of the protein encoded by the atu6002 gene, the function of which is completely unknown. atu6002 expression occurs in agrobacterium-induced tumours, and is also activated on activation of plant cell division by growth hormones. within the expressing plant cells, the atu6002 protein is ta ... | 2013 | 24128370 |
| evaluation of a new high-throughput method for identifying quorum quenching bacteria. | quorum sensing (qs) is a population-dependent mechanism for bacteria to synchronize social behaviors such as secretion of virulence factors. the enzymatic interruption of qs, termed quorum quenching (qq), has been suggested as a promising alternative anti-virulence approach. in order to efficiently identify qq bacteria, we developed a simple, sensitive and high-throughput method based on the biosensor agrobacterium tumefaciens a136. this method effectively eliminates false positives caused by in ... | 2013 | 24121744 |
| differences in differential gene expression between young and mature arabidopsis c58 tumours. | tumorigenesis of plants triggered by agrobacterium tumefaciens has been investigated for over a century, but a global study on changes in gene expression in plant tumours during growth and development has received little attention so far due to technical difficulties. recently a great advance in 'omic' technologies, e.g. microarray, proteome and transcriptome analyses, has allowed differential expression profiling of genes for metabolic regulation during plant tumour growth and development. deek ... | 2014 | 24119138 |
| paracoccidioides brasiliensis pbp27 gene: knockdown procedures and functional characterization. | paracoccidioides brasiliensis pbp27 gene encodes a protein localized in both the fungal cytoplasm and cell wall. the parasitic infectious form produces this protein preferentially with the gene's expression varying between the fungus phylogenetic species. the biological function of the native p27 has yet to be determined during either growth of the yeast or host infection. therefore, in this study, through the use of antisense rna technology and agrobacterium tumefaciens-mediated transformation, ... | 2014 | 24118983 |
| concerted transfer of the virulence ti plasmid and companion at plasmid in the agrobacterium tumefaciens-induced plant tumour. | the plant pathogen agrobacterium tumefaciens c58 harbours three independent type iv secretion (t4ss) machineries. t4sst-dna promotes the transfer of the t-dna to host plant cells, provoking tumour development and accumulation of opines such as nopaline and agrocinopines. t4sspti and t4sspat control the bacterial conjugation of the ti and at plasmids respectively. expression of t4sspti is controlled by the agrocinopine-responsive transcriptional repressor accr. in this work, we compared the genom ... | 2013 | 24118167 |
| diverse profiles of n-acyl-homoserine lactone molecules found in cnidarians. | many marine habitats, such as the surface and tissues of marine invertebrates, including corals, harbour diverse populations of microorganisms, which are thought to play a role in the health of their hosts and influence mutualistic and competitive interactions. investigating the presence and stability of quorum sensing (qs) in these ecosystems may shed light on the roles and control of these bacterial communities. samples of 13 cnidarian species were screened for the presence and diversity of n- ... | 2014 | 24117852 |
| secondary peritonitis due to rhizobium radiobacter. | rhizobium radiobacter rarely causes human infections, most of which develop in immunocompromised patients, and especially those with intravenous catheters. although r. radiobacter has been reported in association with peritonitis, all such reported cases have been due to intra-peritoneal devices in patients undergoing peritoneal dialysis. herein we report the first case of a perforated ulcer complicated by peritonitis caused by r. radiobacter in a healthy adult, which was treated successfully wi ... | 2014 | 24116854 |
| antitumor characterization of various fractions of launaea procumbens. | the antitumor activity of various six fractions of launaea procumbens were studied using the potato disk bioassay technique. three concentrations (10, 100, and 1000 ppm) of all these six fractions were used for characterization of antitumor activity. among these fractions, methanolic fraction exhibited significant inhibition of crown gall tumors caused by agrobacterium tumefaciens followed by butanolic fraction. these results revealed that methanolic and butanolic fractions contain bioactive con ... | 2016 | 24097358 |
| agrobacterium-mediated transformation of promising oil-bearing marine algae parachlorella kessleri. | parachlorella kessleri is a unicellular alga which grows in fresh as well as marine water and is commercially important as biomass/lipid feedstock and in bioremediation. the present study describes the successful transformation of marine p. kessleri with the help of agrobacterium tumefaciens. transformed marine p. kessleri was able to tolerate more than 10 mg l(-1) hygromycin concentration. co-cultivation conditions were modulated to allow the simultaneous growth of both marine p. kessleri and a ... | 2013 | 24097049 |
| the involvement of β-amyrin 28-oxidase (cyp716a52v2) in oleanane-type ginsenoside biosynthesis in panax ginseng. | panax species are the most popular medicinal herbs. the root of these plants contains pharmacologically active triterpene saponins, also known as ginsenosides, compounds that are divided into dammarane- and oleanane-type triterpenes. two cyp716a subfamily genes (cyp716a47 and cyp716a53v2) were recently characterized, encoding an enzyme catalyzing the hydroxylation of dammarane-type triterpenes in panax ginseng. herein, we report that one cyp716a subfamily gene (cyp716a52v2) isolated from p. gins ... | 2013 | 24092881 |
| genome sequence of the quorum-sensing-signal-producing nonpathogen agrobacterium tumefaciens strain p4. | agrobacterium tumefaciens p4 is a quorum-sensing-signal-producing bacterium that has been isolated from the tobacco rhizosphere. this strain belongs to genomospecies 1 of the a. tumefaciens complex; it is avirulent on various putative host plants, devoid of the ti plasmid, and contains a luxi homolog on the at plasmid. | 2013 | 24092790 |
| biological activity of the tzs gene of nopaline agrobacterium tumefaciens gv3101 in plant regeneration and genetic transformation. | agrobacterium tumefaciens has been widely used in plant genetic transformation. hormone-encoding genes residing in the t-dna region have been removed, resulting in disarmed agrobacterium strains that are used in various transformation experiments. nopaline agrobacterium strains, however, carry another hormone gene, trans-zeatin synthesizing (tzs), that codes for trans-zeatin in the virulence region of the tumor-inducing plasmids. we investigated the activity and function of the tzs gene of a nop ... | 2013 | 24088018 |
| stacking resistance to crown gall and nematodes in walnut rootstocks. | crown gall (cg) (agrobacterium tumefaciens) and the root lesion nematodes (rlns) (pratylenchus vulnus) are major challenges faced by the california walnut industry, reducing productivity and increasing the cost of establishing and maintaining orchards. current nematode control strategies include nematicides, crop rotation, and tolerant cultivars, but these methods have limits. developing genetic resistance through novel approaches like rna interference (rnai) can address these problems. rnai-med ... | 2013 | 24083348 |
| an improved plant regeneration and agrobacterium - mediated transformation of red pepper (capsicum annuum l.). | capsicum annuum (red pepper) is an important spice cum vegetable crop in tropical and subtropical countries. here, we report an effective and reproducible auxin free regeneration method for six different red pepper cultivars (aca-10, kashi anmol, lca-235, pbc-535, pusa jwala and supper) using hypocotyl explants and an efficient agrobacterium-mediated transformation protocol. the explants (hypocotyls, cotyledonary leaves and leaf discs) collected from axenic seedlings of six red pepper cultivars ... | 2012 | 24082498 |
| brachypodium sylvaticum, a model for perennial grasses: transformation and inbred line development. | perennial species offer significant advantages as crops including reduced soil erosion, lower energy inputs after the first year, deeper root systems that access more soil moisture, and decreased fertilizer inputs due to the remobilization of nutrients at the end of the growing season. these advantages are particularly relevant for emerging biomass crops and it is projected that perennial grasses will be among the most important dedicated biomass crops. the advantages offered by perennial crops ... | 2013 | 24073248 |
| an orange fluorescent protein tagging system for real-time pollen tracking. | monitoring gene flow could be important for future transgenic crops, such as those producing plant-made-pharmaceuticals (pmps) in open field production. a nicotiana hybrid (nicotiana. tabacum × nicotiana glauca) shows limited male fertility and could be used as a bioconfined pmp platform. effective assessment of gene flow from these plants is augmented with methods that utilize fluorescent proteins for transgenic pollen identification. | 2013 | 24070251 |
| retraction: agrobacterium tumefaciens-mediated transformation and efficient regeneration of a timber yielding plant dalbergia sissoo roxb. | 2013 | 24068859 | |
| [study on human afgf fusion gene transformation with soybean 24 kda oleosin and expression in safflower]. | to investigate the expression of acidic fibroblast growth factor (afgf) in transgenic safflower and lay the foundation for the use of the plant bioreactor large-scale production afgf. | 2013 | 24066580 |
| development of transgenic papaya through agrobacterium-mediated transformation. | transgenic papaya plants were regenerated from hypocotyls and immature zygotic embryo after cocultivation with agrobacterium tumefaciens lba-4404 carrying a binary plasmid vector system containing neomycin phosphotransferase (nptii) gene as the selectable marker and β-glucuronidase (gus) as the reporter gene. the explants were co-cultivated with agrobacterium tumefaciens on regeneration medium containing 500 mg/l carbenicillin + 200 mg/l cefotaxime for one week. the cocultivated explants were tr ... | 2013 | 24066284 |
| agrobacterium tumefaciens-mediated genetic transformation of the taxol-producing endophytic fungus ozonium sp efy21. | an efficient agrobacterium tumefaciens-mediated genetic transformation method was successfully established for a newly isolated taxol-producing fungus, ozonium sp efy21. a specific hygromycin b resistance expression vector, pcambia1304'an7-1, was constructed for fungal transformation. key factors affecting transformation efficiency were thoroughly investigated and optimized. pcr amplification and southern hybridization were used to verify the transformation events. this study should pave the way ... | 2013 | 24065647 |
| trans-kingdom horizontal dna transfer from bacteria to yeast is highly plastic due to natural polymorphisms in auxiliary nonessential recipient genes. | with the rapid accumulation of genomic information from various eukaryotes in the last decade, genes proposed to have been derived from recent horizontal gene transfer (hgt) events have been reported even in non-phagotrophic unicellular and multicellular organisms, but the molecular pathways underlying hgt remain to be explained. the development of in vitro hgt detection systems, which permit the molecular and genetic analyses of donor and recipient organisms and quantify hgt, are helpful in ord ... | 2013 | 24058593 |
| generation and characterization of arabidopsis t-dna insertion mutants. | transfer dna (t-dna) insertion mutants are often used in forward and reverse genetics to reveal the molecular mechanisms of a particular biological process in plants. to generate t-dna insertion mutants, t-dna must be inserted randomly in the genome through transformation mediated by agrobacterium tumefaciens. during generation of a t-dna insertion mutant, agrobacterium competent cells are first prepared and plasmids containing the t-dna introduced into agrobacterium cells. agrobacterium contain ... | 2014 | 24057370 |
| agrobacterium tumefaciens-mediated transient transformation of arabidopsis thaliana leaves. | transient assays provide a convenient alternative to stable transformation. compared to the generation of stably transformed plants, agroinfiltration is more rapid, and samples can be analyzed a few days after inoculation. nevertheless, at difference of tobacco and other plant species, arabidopsis thaliana remains recalcitrant to routine transient assays. in this chapter, we describe a transient expression assay using simple infiltration of intact arabidopsis leaves with agrobacterium tumefacien ... | 2014 | 24057365 |
| antitumour and antioxidant potential of some selected pakistani honeys. | antitumour potential of honey is attributed to its excellent antioxidant activity which in turn depends on the geographical origin. the present study focuses on exploration of antioxidant and antitumour potential as well as total phenolic contents (tpc) of 58 pakistani honeys involving spectrochemical techniques and potato disk assay. agrobacterium tumefaciens was used to induce tumours in potato disks. all analysed honey samples exhibited 1.33±0.00-155.16±0.98mg/100g of tpc, 50% 2,2-diphenyl pi ... | 2014 | 24054252 |
| agrobacterium-mediated transformation of the white-rot fungus physisporinus vitreus. | the biotechnologically important white-rot fungus physisporinus vitreus was co-cultivated with agrobacterium tumefaciens agl-1 carrying plasmids with nourseothricin resistance as the selectable marker gene and red fluorescence protein as a visual marker. mitotically stable transformed isolates were obtained showing red fluorescence protein activity. | 2013 | 24050950 |
| highly efficient synthesis of (r)-3-quinuclidinol in a space-time yield of 916 g l(-1) d(-1) using a new bacterial reductase arqr. | a new keto reductase (arqr), identified from agrobacterium radiobacter ecu2556, can efficiently reduce 3-quinuclidinone in excellent enantioselectivity and high space-time yield for the synthesis of (r)-3-quinuclidinol, a chiral building block of many antimuscarinic agents. this is the first time that a high yield of (r)-3-quinuclidinol up to 916 g l(-1) d(-1) using a bioreduction approach is reported. | 2013 | 24050153 |
| vea and laea transcriptional factors regulate ochratoxin a biosynthesis in aspergillus carbonarius. | ochratoxin a (ota) is a mycotoxin with nephrotoxic, teratogenic and immunotoxic properties which represents a serious risk for human and animal health. aspergillus carbonarius is considered the main ota-producing species in grapes and products such as raisins, wine or juices, although it has also been isolated from coffee, cocoa and cereals. till now not much information is available about regulatory mechanisms of ota production by a. carbonarius. a better understanding of how environmental fact ... | 2013 | 24041999 |
| tropaeolum tops tobacco - simple and efficient transgene expression in the order brassicales. | transient expression systems are valuable tools in molecular biology. agrobacterial infiltration of leaves is well-established in tobacco, but has led to limited success in the model plant arabidopsis thaliana. an efficient expression system combining the advantages of arabidopsis (well-characterised) and the simplicity of leaf infiltration is desirable. here, i describe agrobacterium tumefaciens-mediated transformation of tropaeolummajus (nasturtium, order brassicales) as a remarkably simple, c ... | 2013 | 24039923 |
| agrobacterium-mediated transformation of the winter mushroom, flammulina velutipes. | flammulina velutipes was transformed efficiently by agrobacterium-mediated transformation system. the transformation frequency was about 16% with the gill tissues of the fungal fruiting body. southern hybridization and genetic analysis suggest that the introduced dna was inserted onto different locations of the fungal genome, and inherited stably to the next generation via basidiospores. transformation or gene tagging with agrobacterium t-dna based vector should be useful for wide ranges of gene ... | 2006 | 24039479 |
| celr, an ortholog of the diguanylate cyclase pled of caulobacter, regulates cellulose synthesis in agrobacterium tumefaciens. | cellulose fibrils play a role in attachment of agrobacterium tumefaciens to its plant host. while the genes for cellulose biosynthesis in the bacterium have been identified, little is known concerning the regulation of the process. the signal molecule cyclic di-gmp (c-di-gmp) has been linked to the regulation of exopolysaccharide biosynthesis in many bacterial species, including a. tumefaciens. in this study, we identified two putative diguanylate cyclase genes, celr (atu1297) and atu1060, that ... | 2013 | 24038703 |
| new bacteriocin from bacillus clausii straingm17: purification, characterization, and biological activity. | a bacteriocin-producing strain (9,000 au/ml) was isolated from the rhizosphere of algerian healthy plants ononis angustissima lam. and identified as bacillus clausii strain gm17. the bacteriocin, called bac-gm17, was purified from the culture supernatant after heat treatment, ammonium sulfate precipitation, sephadex g-50 chromatography and mono q fast-performance liquid chromatography (fplc). based on matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis, the puri ... | 2013 | 24037515 |
| high-level extracellular production of d-psicose-3-epimerase with recombinant escherichia coli by a two-stage glycerol feeding approach. | the aim of this study is to achieve high-level extracellular production of d-psicose-3-epimerase (dpe) with recombinant escherichia coli. high-level production of dpe is one of the key factors in d-psicose production. in the present study, the gene aal45544.1 from agrobacterium tumefaciens str. c58 was modified by artificial synthesis for overexpression in e. coli. the total dpe activity reached 3.96 u ml(-1) after optimization of the media composition, induction temperature, and concentration o ... | 2013 | 24037040 |
| unusual spectral properties of bacteriophytochrome agp2 result from a deprotonation of the chromophore in the red-absorbing form pr. | phytochromes are widely distributed photoreceptors with a bilin chromophore that undergo a typical reversible photoconversion between the two spectrally different forms, pr and pfr. the phytochrome agp2 from agrobacterium tumefaciens belongs to the group of bathy phytochromes that have a pfr ground state as a result of the pr to pfr dark conversion. agp2 has untypical spectral properties in the pr form reminiscent of a deprotonated chromophore as confirmed by resonance raman spectroscopy. uv/vis ... | 2013 | 24036118 |
| genetic diversity of siderophore-producing bacteria of tobacco rhizosphere. | the genetic diversity of siderophore-producing bacteria of tobacco rhizosphere was studied by amplified ribosomal dna restriction analysis (ardra), 16s rrna sequence homology and phylogenetics analysis methods. studies demonstrated that 85% of the total 354 isolates produced siderophores in iron limited liquid medium. a total of 28 ardra patterns were identified among the 299 siderophore-producing bacterial isolates. the 28 ardra patterns represented bacteria of 14 different genera belonging to ... | 2009 | 24031358 |
| indole inhibits bacterial quorum sensing signal transmission by interfering with quorum sensing regulator folding. | quorum sensing (qs)-dependent biofilm formation and motility were controlled by aqsr in acinetobacter oleivorans dr1. qs-controlled phenotypes appeared to be inhibited by indole and the aqsr mutant had the same phenotypes. we demonstrated that the turnover rate of aqsr became more rapid without the n-acylhomoserine lactone (ahl) signal, and that indole could increase the expression of many protease and chaperone proteins. the addition of exogenous indole decreased the expression of two aqsr-targ ... | 2013 | 24025605 |
| construction of an engineering strain producing high yields of α-transglucosidase via agrobacterium tumefaciens-mediated transformation of asperillus niger. | in this study, agrobacterium tumefaciens-mediated transformation (atmt) was used in breeding industrial strains for the purpose of improving α-transglucosidase production. firstly, an efficient atmt system for asperillus niger was established by optimization of several influencing factors, in which transformation efficiency was improved up to 14-fold compared with the initial conditions. furthermore, binary vector pbi-glu containing an α-transglucosidase expression cassette was constructed and t ... | 2013 | 24018680 |
| [analytical methods for control of foodstuffs made from bioengineered plants]. | foodstuffs made by modern biotechnology are requiring for special control. analytical methods used for these purposes are being constantly perfected. when choosing a strategy for the analysis, several factors have to be assessed: specificity, sensitivity, practically of the method and time efficiency. to date, the gmo testing methods are mainly based on the inserted dna sequences and newly produced proteins in gmos. protein detection methods are based mainly on elisa. the specific detection of a ... | 2013 | 24006752 |
| host range diversification within the incp-1 plasmid group. | broad-host-range plasmids play a critical role in the spread of antibiotic resistance and other traits. in spite of increasing information about the genomic diversity of closely related plasmids, the relationship between sequence divergence and host range remains unclear. incp-1 plasmids are currently classified into six subgroups based on the genetic distance of backbone genes. we investigated whether plasmids from two subgroups exhibit a different host range, using two incp-1γ plasmids, an inc ... | 2013 | 24002747 |
| increased 1-aminocyclopropane-1-carboxylate deaminase activity enhances agrobacterium tumefaciens-mediated gene delivery into plant cells. | agrobacterium-mediated transformation is a useful tool for the genetic modification in plants, although its efficiency is low for several plant species. agrobacterium-mediated transformation has three major steps in laboratory-controlled experiments: the delivery of t-dna into plant cells, the selection of transformed plant cells, and the regeneration of whole plants from the selected cells. each of these steps must be optimized to improve the efficiency of agrobacterium-mediated plant transform ... | 2013 | 24000136 |
| demonstration of crispr/cas9/sgrna-mediated targeted gene modification in arabidopsis, tobacco, sorghum and rice. | the type ii crispr/cas system from streptococcus pyogenes and its simplified derivative, the cas9/single guide rna (sgrna) system, have emerged as potent new tools for targeted gene knockout in bacteria, yeast, fruit fly, zebrafish and human cells. here, we describe adaptations of these systems leading to successful expression of the cas9/sgrna system in two dicot plant species, arabidopsis and tobacco, and two monocot crop species, rice and sorghum. agrobacterium tumefaciens was used for delive ... | 2013 | 23999092 |
| transient gene expression in medicago truncatula leaves via agroinfiltration. | transient expression is a powerful method for the functional characterization of genes. in this chapter, we outline a protocol for the transient expression of constructs in medicago truncatula leaves using agrobacterium tumefaciens infiltration. using quantitative real-time pcr we demonstrate that the infiltration of a construct containing the legume anthocyanin production 1 (lap1) transcription factor results in the strong upregulation of key biosynthetic genes and the accumulation of anthocyan ... | 2013 | 23996318 |
| stable transformation of medicago truncatula cv. jemalong for gene analysis using agrobacterium tumefaciens. | medicago truncatula is a model legume that has all the genomic resources to be suitable as a model for functional genomics. transformation to produce transgenic plants is part of this toolkit, enabling a spectrum of approaches to study gene function: including knockdown, overexpression, reporter genes for gene expression, and proteins tagged with fluorescent proteins such as gfp. a special genetic line is necessary for transformation and jemalong 2ha derived from cv. jemalong is used in the meth ... | 2013 | 23996317 |
| applying horizontal gene transfer phenomena to enhance non-viral gene therapy. | horizontal gene transfer (hgt) is widespread amongst prokaryotes, but eukaryotes tend to be far less promiscuous with their genetic information. however, several examples of hgt from pathogens into eukaryotic cells have been discovered and mimicked to improve non-viral gene delivery techniques. for example, several viral proteins and dna sequences have been used to significantly increase cytoplasmic and nuclear gene delivery. plant genetic engineering is routinely performed with the pathogenic b ... | 2013 | 23994344 |
| efficient gene targeting in penicillium chrysogenum using novel agrobacterium-mediated transformation approaches. | the industrial production of β-lactam antibiotics by penicillium chrysogenum has increased tremendously over the last decades, however, further optimization via classical strain and process improvement has reached its limits. the availability of the genome sequence provides new opportunities for directed strain improvement, but this requires the establishment of an efficient gene targeting (gt) system. recently, mutations affecting the non-homologous end joining (nhej) pathway were shown to incr ... | 2013 | 23994321 |
| molecular tools for functional genomics in filamentous fungi: recent advances and new strategies. | advances in genetic transformation techniques have made important contributions to molecular genetics. various molecular tools and strategies have been developed for functional genomic analysis of filamentous fungi since the first dna transformation was successfully achieved in neurospora crassa in 1973. increasing amounts of genomic data regarding filamentous fungi are continuously reported and large-scale functional studies have become common in a wide range of fungal species. in this review, ... | 2013 | 23988676 |
| antitumor, antioxidant and antimicrobial studies of substituted pyridylguanidines. | a series of n-pivaloyl-n'-(alkyl/aryl)-n''-pyridylguanidine of general formula c₄h₉conhc(nr¹r²)npy have been synthesized and characterized using elemental analysis, ft-ir, multinuclear nmr spectroscopy, and in the case of compounds 7 and 11, by single crystal x-ray diffraction (xrd). the synthesized guanidines were tested for antitumor activities against potato tumor, and showed excellent inhibition against agrobacterium tumefaciens (at10)-induced tumor. the antioxidant and antimicrobial activit ... | 2013 | 23985956 |
| genetic transformation in citrus. | citrus is one of the world's important fruit crops. recently, citrus molecular genetics and biotechnology work have been accelerated in the world. genetic transformation, a biotechnological tool, allows the release of improved cultivars with desirable characteristics in a shorter period of time and therefore may be useful in citrus breeding programs. citrus transformation has now been achieved in a number of laboratories by various methods. agrobacterium tumefaciens is used mainly in citrus tran ... | 2013 | 23983635 |
| live-cell imaging of microtubules and microtubule-associated proteins in arabidopsis thaliana. | microtubules and microtubule-associated proteins (maps) play fundamental roles in plant growth and morphogenesis. the ability to observe microtubules and maps in living cells using fluorescent protein fusions has propelled plant scientists forward and given them the opportunity to answer longstanding biological questions. in combination with the genetic resources available in the model plant arabidopsis thaliana, our mechanistic understanding of how the microtubule cytoskeleton affects plant lif ... | 2013 | 23973076 |
| functional analysis of autophagy genes via agrobacterium-mediated transformation in the vascular wilt fungus verticillium dahliae. | autophagy is a widely conserved intracellular process for degradation and recycling of proteins, organelles and cytoplasm in eukaryotic organisms and is now emerging as an important process in foliar infection by many plant pathogenic fungi. however, the role of autophagy in soil-borne fungal physiology and infection biology is poorly understood. here, we report the establishment of an agrobacterium tumefaciens-mediated transformation (atmt) system and its application to investigate two autophag ... | 2013 | 23969251 |