Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| blurred lines: pathogens, commensals, and the healthy gut. | 2015 | 26664968 | |
| identification of the main quinolone resistance determinant in campylobacter jejuni and campylobacter coli by mama-deg pcr. | among zoonotic diseases, campylobacteriosis stands out as the major bacterial infection producing human gastroenteritis. antimicrobial therapy, only recommended in critical cases, is challenged by resistance mechanisms that should be unambiguously detected for achievement of effective treatments. quinolone (ciprofloxacin) resistance of campylobacter jejuni and campylobacter coli, the 2 main campylobacter detected in humans, is conferred by the mutation gyra c-257-t, which can be genotyped by sev ... | 2016 | 26658311 |
| comparative evaluation of the antimicrobial activity of different antimicrobial peptides against a range of pathogenic bacteria. | the rapid emergence of resistance to classical antibiotics has increased the interest in novel antimicrobial compounds. antimicrobial peptides (amps) represent an attractive alternative to classical antibiotics and a number of different studies have reported antimicrobial activity data of various amps, but there is only limited comparative data available. the mode of action for many amps is largely unknown even though several models have suggested that the lipopolysaccharides (lps) play a crucia ... | 2015 | 26656394 |
| the sweet branch of metabolic engineering: cherry-picking the low-hanging sugary fruits. | in the first science review on the then nascent metabolic engineering field in 1991, dr. james e. bailey described how improving erythropoietin (epo) glycosylation can be achieved via metabolic engineering of chinese hamster ovary (cho) cells. in the intervening decades, metabolic engineering has brought sweet successes in glycoprotein engineering, including antibodies, vaccines, and other human therapeutics. today, not only eukaryotes (cho, plant, insect, yeast) are being used for manufacturing ... | 2015 | 26655367 |
| sensitive detection of campylobacter jejuni using nanoparticles enhanced qcm sensor. | a quartz crystal microbalance (qcm) sensor platform was used to develop an immunosensor for the detection of food pathogen campylobacter jejuni. rabbit polyclonal antibodies and commercially available mouse monoclonal antibodies against c. jejuni were investigated to construct direct, sandwich and gold-nanoparticles (aunps) amplified sandwich assays. the performance of the qcm immunosensor developed using sandwich assay by utilising the rabbit polyclonal antibody as the capture antibody and conj ... | 2016 | 26649490 |
| epidemiology, detection, and control of foodborne microbial pathogens. | 2015 | 26649309 | |
| persistent microbial dysbiosis in preterm premature rupture of membranes from onset until delivery. | background. preterm premature rupture of membranes (pprom) is a major leading cause of preterm births. while the cause for pprom remains unidentified, it is anticipated to be due to subclinical infection, since a large proportion of pprom patients display signs of chorioamnionitis. since subclinical infections can be facilitated by dysbiosis, our goal was to characterize the vaginal microbiome and amniotic fluid discharge upon pprom, through latency antibiotic treatment, and until delivery, to d ... | 2015 | 26644969 |
| immunoglobulin g response in patients with campylobacter concisus diarrhea. | limited information is available on the systemic immunoglobulin response in patients infected with the emerging pathogen campylobacter concisus. the aim of the present study was to detect anti-c. concisus antibodies in serum of 88 patients with c. concisus gastroenteritis. specific igg antibodies to c. concisus were measured in serum using an in-house enzyme-linked immunosorbent assay, and pooled donor serum was used as a control. the mean optical density was 0.135 (sem: 0.020) for the 88 adult ... | 2016 | 26643061 |
| how the sequence of a gene specifies structural symmetry in proteins. | internal symmetry is commonly observed in the majority of fundamental protein folds. meanwhile, sufficient evidence suggests that nascent polypeptide chains of proteins have the potential to start the co-translational folding process and this process allows mrna to contain additional information on protein structure. in this paper, we study the relationship between gene sequences and protein structures from the viewpoint of symmetry to explore how gene sequences code for structural symmetry in p ... | 2015 | 26641668 |
| repairing oxidized proteins in the bacterial envelope using respiratory chain electrons. | the reactive species of oxygen and chlorine damage cellular components, potentially leading to cell death. in proteins, the sulfur-containing amino acid methionine is converted to methionine sulfoxide, which can cause a loss of biological activity. to rescue proteins with methionine sulfoxide residues, living cells express methionine sulfoxide reductases (msrs) in most subcellular compartments, including the cytosol, mitochondria and chloroplasts. here we report the identification of an enzymati ... | 2015 | 26641313 |
| molecular genotyping of giardia duodenalis isolates from symptomatic individuals attending two major public hospitals in madrid, spain. | the flagellate protozoan giardia duodenalis is an enteric parasite causing human giardiasis, a major gastrointestinal disease of global distribution affecting both developing and industrialised countries. in spain, sporadic cases of giardiasis have been regularly identified, particularly in pediatric and immigrant populations. however, there is limited information on the genetic variability of circulating g. duodenalis isolates in the country. | 2015 | 26641082 |
| my 40-year history with cronobacter/enterobacter sakazakii - lessons learned, myths debunked, and recommendations. | much has been learned about organism in the cronobacter/enterobacter sakazakii complex since i first named and described enterobacter sakazakii in 1980. however, there are still wide knowledge gaps. one of the most serious is that are still many uncertainties associated with assessing the public health risk posed by these bacteria, particularly in neonatal meningitis. over the last few decades, cronobacter contamination of commercial powdered infant formula products has apparently been reduced, ... | 2015 | 26640778 |
| genomic features of environmental and clinical vibrio parahaemolyticus isolates lacking recognized virulence factors are dissimilar. | vibrio parahaemolyticus is a bacterial pathogen that can cause illness after the consumption or handling of contaminated seafood. the primary virulence factors associated with v. parahaemolyticus illness are thermostable direct hemolysin (tdh) and tdh-related hemolysin (trh). however, clinical strains lacking tdh and trh have recently been isolated, and these clinical isolates are poorly understood. to help understand the emergence of clinical tdh- and trh-negative isolates, a genomic approach w ... | 2016 | 26637607 |
| occupational exposure to swine, poultry, and cattle and antibody biomarkers of campylobacter jejuni exposure and autoimmune peripheral neuropathy. | foodborne campylobacter jejuni infection has been associated with an increased risk of autoimmune peripheral neuropathy, but risks of occupational exposure to c. jejuni have received less attention. this study compared anti-c. jejuni iga, igg, and igm antibody levels, as well as the likelihood of testing positive for any of five anti-ganglioside autoantibodies, between animal farmers and non-farmers. anti-c. jejuni antibody levels were also compared between farmers with different animal herd or ... | 2015 | 26636679 |
| [potential use of mass spectrometry for subtyping of campylobacter]. | molecular epidemiology is a field that uses results of typing techniques to obtain information on detailed characterization of bacterial strains for determining the identity, similarity or difference in bacteria of the same genus, species or serotype. nowadays, the most commonly used methods are based on monitoring differences in bacterial genotypes. however, most of these techniques are time-consuming and costly. a method increasingly used in routine microbiological testing is matrix-assisted l ... | 2015 | 26636631 |
| interaction of intestinal microorganisms with the human host in the framework of autoimmune diseases. | autoimmune diseases, such as systemic lupus erythematosus (sle), are caused by a complex interaction of environmental-, genetic-, and sex-related factors. although sle has traditionally been considered independent from the microbiota, recent work published during the last 5 years suggests a strong connection between sle and the composition of our gut commensals as one of the main environmental factors linked to this disease. preliminary data have evidenced that (i) interaction of certain microbi ... | 2015 | 26635808 |
| elucidating host-pathogen interactions based on post-translational modifications using proteomics approaches. | microbes with the capability to survive in the host tissue and efficiently subvert its innate immune responses can cause various health hazards. there is an inherent need to understand microbial infection patterns and mechanisms in order to develop efficient therapeutics. microbial pathogens display host specificity through a complex network of molecular interactions that aid their survival and propagation. co-infection states further lead to complications by increasing the microbial burden and ... | 2015 | 26635773 |
| important role of a putative lytic transglycosylase cj0843c in β-lactam resistance in campylobacter jejuni. | beta-lactam antibiotics are an important class of antibiotics for treating bacterial infections. despite prevalent β-lactam resistance in campylobacter jejuni, the leading bacterial cause of human diarrhea in developed countries, molecular mechanism of β-lactam resistance in c. jejuni is still largely unknown. in this study, c. jejuni 81-176 was used for random transposon mutagenesis. screening of a 2,800-mutant library identified 22 mutants with increased susceptibility to ampicillin. of these ... | 2015 | 26635760 |
| campylobacter jejuni sequence types show remarkable spatial and temporal stability in blackbirds. | the zoonotic bacterium campylobacter jejuni has a broad host range but is especially associated with birds, both domestic and wild. earlier studies have indicated thrushes of the genus turdus in europe to be frequently colonized with c. jejuni, and predominately with host-associated specific genotypes. the european blackbird turdus merula has a large distribution in europe, including some oceanic islands, and was also introduced to australia by european immigrants in the 1850s. | 2015 | 26634844 |
| updated campylobacter jejuni capsule pcr multiplex typing system and its application to clinical isolates from south and southeast asia. | campylobacter jejuni produces a polysaccharide capsule that is the major determinant of the penner serotyping scheme. this passive slide agglutination typing system was developed in the early 1980's and was recognized for over two decades as the gold standard for c. jejuni typing. a preliminary multiplex pcr technique covering 17 serotypes was previously developed in order to replace this classic serotyping scheme. here we report the completion of the multiplex pcr technology that is able to ide ... | 2015 | 26630669 |
| utilizing cmp-sialic acid analogs to unravel neisseria gonorrhoeae lipooligosaccharide-mediated complement resistance and design novel therapeutics. | neisseria gonorrhoeae deploys a novel immune evasion strategy wherein the lacto-n-neotetraose (lnnt) structure of lipooligosaccharide (los) is capped by the bacterial sialyltransferase, using host cytidine-5'-monophosphate (cmp)-activated forms of the nine-carbon nonulosonate (nulo) sugar n-acetyl-neuraminic acid (neu5ac), a sialic acid (sia) abundant in humans. this allows evasion of complement-mediated killing by recruiting factor h (fh), an inhibitor of the alternative complement pathway, and ... | 2015 | 26630657 |
| x-ray-induced catalytic active-site reduction of a multicopper oxidase: structural insights into the proton-relay mechanism and o2-reduction states. | during x-ray data collection from a multicopper oxidase (mco) crystal, electrons and protons are mainly released into the system by the radiolysis of water molecules, leading to the x-ray-induced reduction of o2 to 2h2o at the trinuclear copper cluster (tnc) of the enzyme. in this work, 12 crystallographic structures of thermus thermophilus hb27 multicopper oxidase (tth-mco) in holo, apo and hg-bound forms and with different x-ray absorbed doses have been determined. in holo tth-mco structures w ... | 2015 | 26627648 |
| molecular characterization, antimicrobial resistance and caco-2 cell invasion potential of campylobacter jejuni/coli from young children with diarrhea. | campylobacter is a major cause of bacterial gastroenteritis worldwide. young children represent a particular age group affected by campylobacter infection because of their limited diets and weak immune systems. | 2016 | 26627197 |
| fosfomycin: uses and potentialities in veterinary medicine. | fosfomycin (fos) is a natural bactericidal broad-spectrum antibiotic which acts on proliferating bacteria by inhibiting cell wall and early murein/peptidoglycan synthesis. bactericidal activity is evident against gram positive and gram negative bacteria and can also act synergistically with other antibiotics. bacterial resistance to fos may be natural or acquired. other properties of this drug include inhibition of bacterial adhesion to epithelial cells, exopolysaccharide biofilm penetration, im ... | 2014 | 26623336 |
| bacterial exotoxins and the inflammasome. | the inflammasomes are intracellular protein complexes that play an important role in innate immune sensing. activation of inflammasomes leads to activation of caspase-1 and maturation and secretion of the pro-inflammatory cytokines interleukin (il)-1β and il-18. in certain myeloid cells, this activation can also lead to an inflammatory cell death (pyroptosis). inflammasome sensor proteins have evolved to detect a range of microbial ligands and bacterial exotoxins either through direct interactio ... | 2015 | 26617605 |
| survival with a helping hand: campylobacter and microbiota. | campylobacteriosis is the most important bacterial food-borne disease in the developed world. consumption of chicken meat, beef or raw milk, direct contact with ruminants and exposure to contaminated surface water or even consumption of tap water have been identified as risk factors for human disease. however, the most important risk factor is consumption of and/or handling contaminated chicken. campylobacter spp. are fastidious microorganisms but must somehow survive outside the host, especiall ... | 2015 | 26617600 |
| high prevalence of hyper-aerotolerant campylobacter jejuni in retail poultry with potential implication in human infection. | campylobacter jejuni is a leading cause of foodborne illnesses around the world. since c. jejuni is microaerophilic and sensitive to oxygen, aerotolerance is important in the transmission of c. jejuni to humans via foods under aerobic conditions. in this study, 70 c. jejuni strains were isolated from retail raw chicken meats and were subject to multilocus sequence typing (mlst) analysis. in the aerotolerance testing by aerobic shaking at 200 rpm, 50 (71.4%) isolates survived after 12 h (i.e., ae ... | 2015 | 26617597 |
| antimicrobial resistance and genotypic diversity of campylobacter isolated from pigs, dairy, and beef cattle in tanzania. | foodborne campylobacter infections pose a serious threat to public health worldwide. however, the occurrence and characteristics of campylobacter in food animals and products remain largely unknown in tanzania. the objective of this study was to determine the prevalence, antibiotic resistance, and genetic profiles (sequence types, sts) of campylobacter isolated from feces of pigs and dairy and beef cattle in tanzania. overall, 259 (~30%) of 864 samples were positive for campylobacter spp, which ... | 2015 | 26617582 |
| parasitic diarrheal disease: drug development and targets. | diarrhea is the manifestation of gastrointestinal infection and is one of the major causes of mortality and morbidity specifically among the children of less than 5 years age worldwide. moreover, in recent years there has been a rise in the number of reports of intestinal infections continuously in the industrialized world. these are largely related to waterborne and food borne outbreaks. these occur by the pathogenesis of both prokaryotic and eukaryotic organisms like bacteria and parasites. th ... | 2015 | 26617574 |
| guillain barré syndrome (gbs): new insights in the molecular mimicry between c. jejuni and human peripheral nerve (hpn) proteins. | profile and immunoreactivity of proteins from hpn tissue, and from campylobacter jejuni (o:19) were investigated. proteins were extracted, separated by sds-page, their cross reactivity monitored by western blotting, and identified by nhplc-nesi-hrms analysis. proteins from c. jejuni, at mw ~70 kda were chaperone/co-chaperone proteins (groel, dnak and htpg). in the corresponding hpn band were serum albumin, neurofilament light peptide, cytoskeletal keratins and one hsp 70 and one hsp60. these cha ... | 2015 | 26616887 |
| guillain-barre syndrome following dengue fever and literature review. | dengue is an arboviral infection that classically presents with fever, joint pain, headaches, skin flush and morbilliform rashes. the incidence of neurological symptoms and complications in dengue varies from 1 to 25% that include encephalopathy, guillain-barre syndrome (gbs), acute motor weakness, seizures, neuritis, hypokalaemic paralysis, pyramidal tract signs, and a few more. dengue fever as an antecedent infection in gbs is uncommon. | 2015 | 26613722 |
| crystal structure and comparative sequence analysis of gmha from colwellia psychrerythraea strain 34h provides insight into functional similarity with diaa. | the psychrophilic organism colwellia psychrerythraea strain 34h produces extracellular polysaccharide substances to tolerate cold environments. sedoheptulose 7-phosphate isomerase (gmha) is essential for producing d-glycero-d-mannoheptose 7-phosphate, a key mediator in the lipopolysaccharide biosynthetic pathway. we determined the crystal structure of gmha from c. psychrerythraea strain 34h (cpsgmha, uniprotkb code: q47vu0) at a resolution of 2.8 å. the tetrameric structure is similar to that of ... | 2015 | 26612680 |
| cultured enterocytes internalise bacteria across their basolateral surface for, pathogen-inhibitable, trafficking to the apical compartment. | in vitro- and in vivo-polarised absorptive epithelia (enterocytes) are considered to be non-phagocytic towards bacteria with invasive pathogenic strains relying on virulence factors to 'force' entry. here, we report a serendipitous discovery that questions these beliefs. thus, we uncover in well-established models of human small (caco-2; tc-7) and large (t84) intestinal enterocytes a polarization-dependent mechanism that can transfer millions of bacteria from the basal to apical compartment. ant ... | 2015 | 26612456 |
| survey of physicochemical characteristics and microbial contamination in selected food locally vended in morogoro municipality, tanzania. | raw milk, raw fruit juice and raw fish are enriched with essential nutrients for human diet but are prone to microbial contamination along the value chain. this cross sectional study was conducted to assess physicochemical characteristics and microbial quality of raw milk, fruit juice and fish from food vendors in morogoro municipality, tanzania. the physicochemical assessment of food samples was done by smell, colour, presence of debris, turbidity, consistence, ph and clot on alcohol test. hygi ... | 2015 | 26611537 |
| functional analysis of n-linking oligosaccharyl transferase enzymes encoded by deep-sea vent proteobacteria. | bacterial n-linking oligosaccharyl transferases (otase enzymes) transfer lipid-linked glycans to selected proteins in the periplasm and were first described in the intestinal pathogen campylobacter jejuni, a member of the ε-proteobacteria-subdivision of bacteria. more recently, orthologues from other ε-proteobacterial campylobacter and helicobacter species and a δ-proteobacterium, desulfovibrio desulfuricans, have been described, suggesting that these two subdivisions of bacteria may be a source ... | 2015 | 26610891 |
| functional analysis of n-linking oligosaccharyl transferase enzymes encoded by deep-sea vent proteobacteria. | bacterial n-linking oligosaccharyl transferases (otase enzymes) transfer lipid-linked glycans to selected proteins in the periplasm and were first described in the intestinal pathogen campylobacter jejuni, a member of the ε-proteobacteria-subdivision of bacteria. more recently, orthologues from other ε-proteobacterial campylobacter and helicobacter species and a δ-proteobacterium, desulfovibrio desulfuricans, have been described, suggesting that these two subdivisions of bacteria may be a source ... | 2015 | 26610891 |
| pseudomonas aeruginosa facilitates campylobacter jejuni growth in biofilms under oxic flow conditions. | we investigated the growth of campylobacter jejuni in biofilms with pseudomonas aeruginosa under oxic flow conditions. we observed the growth of c. jejuni in mono-culture, deposited on pre-established p. aeruginosa biofilms, and co-inoculated with p. aeruginosa. in mono-culture, c. jejuni was unable to form biofilms. however, deposited c. jejuni continuously grew on pre-established p. aeruginosa biofilms for a period of 3 days. the growth of scattered c. jejuni clusters was strictly limited to t ... | 2015 | 26610432 |
| reads2type: a web application for rapid microbial taxonomy identification. | identification of bacteria may be based on sequencing and molecular analysis of a specific locus such as 16s rrna, or a set of loci such as in multilocus sequence typing. in the near future, healthcare institutions and routine diagnostic microbiology laboratories may need to sequence the entire genome of microbial isolates. therefore we have developed reads2type, a web-based tool for taxonomy identification based on whole bacterial genome sequence data. | 2015 | 26608174 |
| complete genome sequence of campylobacter jejuni rm1285, a rod-shaped morphological variant. | campylobacter jejuni is a spiral shaped gram-negative food-borne bacterial pathogen of humans found on poultry products. strain rm1285 is a rod-shaped variant of this species. the genome of rm1285 was determined to be 1,635,803 bp, with a g+c content of 30.5%. | 2015 | 26607886 |
| differential roles of the hemerythrin-like proteins of mycobacterium smegmatis in hydrogen peroxide and erythromycin susceptibility. | hemerythrin-like proteins are oxygen-carrying non-heme di-iron binding proteins and their functions have effect on oxidation-reduction regulation and antibiotic resistance. recent studies using bioinformatic analyses suggest that multiple hemerythrin-like protein coding sequences might have been acquired by lateral gene transfer and the number of hemerythrin-like proteins varies amongst different species. mycobacterium smegmatis contains three hemerythrin-like proteins, msmeg_3312, msmeg_2415 an ... | 2015 | 26607739 |
| draft genome sequence of janthinobacterium lividum strain mtr reveals its mechanism of capnophilic behavior. | janthinobacterium lividum is a gram-negative bacterium able to produce violacein, a pigment with antimicrobial and antitumor properties. janthinobacterium lividum colonizes the skin of some amphibians and confers protection against fungal pathogens. the mechanisms underlying this association are not well understood. in order to identify the advantages for the bacterium to colonize amphibian skin we sequenced janthinobacterium lividum strain mtr, a strain isolated from cajón del maipo, chile. the ... | 2015 | 26605004 |
| probiotics, prebiotics and synbiotics- a review. | the health benefits imparted by probiotics and prebiotics as well as synbiotics have been the subject of extensive research in the past few decades. these food supplements termed as functional foods have been demonstrated to alter, modify and reinstate the pre-existing intestinal flora. they also facilitate smooth functions of the intestinal environment. most commonly used probiotic strains are: bifidobacterium, lactobacilli, s. boulardii, b. coagulans. prebiotics like fos, gos, xos, inulin; fru ... | 2015 | 26604335 |
| seroprevalence in chickens against campylobacter jejuni flagellar capping protein (flid) in selected areas of the united states. | campylobacter jejuni is a causative pathogen of human acute bacterial gastroenteritis. infected poultry products are regarded as a major source for human c. jejuni infection. the flagellar capping protein (flid) is highly conserved among c. jejuni strains/isolates and is antigenic as analysed by immunoblot. in this study, we used the flid protein as a probe to survey the prevalence of c. jejuni antibodies in chickens from two areas in the united states. a total of 394 samples were tested. sera f ... | 2016 | 26603949 |
| comparative genomics and genome biology of invasive campylobacter jejuni. | campylobacter jejuni is a major pathogen in bacterial gastroenteritis worldwide and can cause bacteremia in severe cases. c. jejuni is highly structured into clonal lineages of which the st677cc lineage has been overrepresented among c. jejuni isolates derived from blood. in this study, we characterized the genomes of 31 c. jejuni blood isolates and 24 faecal isolates belonging to st677cc in order to study the genome biology related to c. jejuni invasiveness. we combined the genome analyses with ... | 2015 | 26603914 |
| isolation and molecular identification of potentially pathogenic escherichia coli and campylobacter jejuni in feral pigeons from an urban area in the city of lima, peru. | feral pigeons (columbia livia) live in close contact with humans and other animals. they can transmit potentially pathogenic and zoonotic agents. the objective of this study was to isolate and detect strains of diarrheagenic escherichia coli and campylobacter jejuni of urban feral pigeons from an area of lima, peru. fresh dropping samples from urban parks were collected for microbiological isolation of e. coli strains in selective agar, and campylobacter by filtration method. molecular identific ... | 2015 | 26603225 |
| conservation and covariance in small bacterial phosphoglycosyltransferases identify the functional catalytic core. | phosphoglycosyltransferases (pgts) catalyze the transfer of a c1'-phosphosugar from a soluble sugar nucleotide diphosphate to a polyprenol phosphate. these enzymes act at the membrane interface, forming the first membrane-associated intermediates in the biosynthesis of cell-surface glycans and glycoconjugates, including glycoproteins, glycolipids, and the peptidoglycan in bacteria. pgts vary greatly in both their membrane topologies and their substrate preferences. pgts, such as mray and weca, a ... | 2015 | 26600273 |
| the gut-brain axis: the missing link in depression. | the gut microbiota is essential to human health and the immune system and plays a major role in the bidirectional communication between the gut and the brain. based on evidence, the gut microbiota is associated with metabolic disorders such as obesity, diabetes mellitus and neuropsychiatric disorders such as schizophrenia, autistic disorders, anxiety disorders and major depressive disorders. in the past few years, neuroscientific research has shown the importance of the microbiota in the develop ... | 2015 | 26598580 |
| description of campylobacter jejuni bf, an atypical aero-tolerant strain. | campylobacter jejuni is a leading cause of bacterial enteritis worldwide. this microaerophilic bacterium can survive in aerobic environments, suggesting it has protective mechanisms against oxidative stress. the clinical c. jejuni bf strain is characterized by an increased resistance to oxygen. this study aimed to characterize the behavior of the clinical c. jejuni bf strain under an aerobic atmosphere and in response to ros-promoter agents. | 2015 | 26594244 |
| a seventeen-year observation of the antimicrobial susceptibility of clinical campylobacter jejuni and the molecular mechanisms of erythromycin-resistant isolates in beijing, china. | to investigate the dynamic development of the antimicrobial resistance of campylobacter jejuni isolated from human diarrhea in beijing, china, between 1994 and 2010, and to further analyze the molecular mechanisms of erythromycin-resistant strains. | 2016 | 26594011 |
| diversity and relatedness of shiga toxin-producing escherichia coli and campylobacter jejuni between farms in a dairy catchment. | the aim of this study was to examine the population structure, transmission and spatial relationship between genotypes of shiga toxin-producing escherichia coli (stec) and campylobacter jejuni, on 20 dairy farms in a defined catchment. pooled faecal samples (n = 72) obtained from 288 calves were analysed by real-time polymerase chain reaction (rtpcr) for e. coli serotypes o26, o103, o111, o145 and o157. the number of samples positive for e. coli o26 (30/72) was high compared to e. coli o103 (7/7 ... | 2016 | 26593403 |
| influence of enteric bacteria, parasite infections and nutritional status on diarrhoea occurrence among 6-60 months old children admitted at a regional hospital in morogoro, tanzania. | while nutritional, microbiological and immunological factors have been implicated in childhood diarrhoea in many countries, there is limited aetiological information in morogoro region of tanzania. a case-control study was conducted to establish whether diarrhoea in 6-60 months old children admitted at a regional hospital in morogoro, was attributable to enteric bacteria and/or parasites and the contribution of under-nutrition, as measured by weight-for-age below -2 sd. from january to september ... | 2012 | 26591731 |
| colombos v3.0: leveraging gene expression compendia for cross-species analyses. | colombos is a database that integrates publicly available transcriptomics data for several prokaryotic model organisms. compared to the previous version it has more than doubled in size, both in terms of species and data available. the manually curated condition annotation has been overhauled as well, giving more complete information about samples' experimental conditions and their differences. functionality-wise cross-species analyses now enable users to analyse expression data for all species ... | 2015 | 26586805 |
| colombos v3.0: leveraging gene expression compendia for cross-species analyses. | colombos is a database that integrates publicly available transcriptomics data for several prokaryotic model organisms. compared to the previous version it has more than doubled in size, both in terms of species and data available. the manually curated condition annotation has been overhauled as well, giving more complete information about samples' experimental conditions and their differences. functionality-wise cross-species analyses now enable users to analyse expression data for all species ... | 2015 | 26586805 |
| the type 2 dutpase of bacteriophage ϕnm1 initiates mobilization of staphylococcus aureus bovine pathogenicity island 1. | staphylococcus aureus pathogenicity islands (sapis) are genetic elements that are mobilized by specific helper phages. the initial step in mobilization is the derepression of the sapi by the interaction of a phage protein with the sapi master repressor stl. stl proteins are highly divergent between different sapis and respond to different phage-encoded derepressors. one such sapi, sapibov1, is derepressed by the dutpase (dut) of bacteriophage 80α (dut80α) and its phage ϕ11 homolog, dut11. we pre ... | 2015 | 26585401 |
| the type 2 dutpase of bacteriophage ϕnm1 initiates mobilization of staphylococcus aureus bovine pathogenicity island 1. | staphylococcus aureus pathogenicity islands (sapis) are genetic elements that are mobilized by specific helper phages. the initial step in mobilization is the derepression of the sapi by the interaction of a phage protein with the sapi master repressor stl. stl proteins are highly divergent between different sapis and respond to different phage-encoded derepressors. one such sapi, sapibov1, is derepressed by the dutpase (dut) of bacteriophage 80α (dut80α) and its phage ϕ11 homolog, dut11. we pre ... | 2015 | 26585401 |
| comprehensive analysis of flagellin glycosylation in campylobacter jejuni nctc 11168 reveals incorporation of legionaminic acid and its importance for host colonization. | campylobacter jejuni is the leading cause of bacterial gastroenteritis. it relies on several virulence factors for host colonization, including glycosylated flagella. c. jejuni nctc 11168 modifies its flagellins with pseudaminic acid derivatives. it is also presumed to modify these proteins with legionaminic acid, although no glycopeptide evidence was available at the onset of this study. the enzyme encoded by cj1319 can be used to make legionaminic acid in vitro, but the pathway for legionamini ... | 2016 | 26582606 |
| eric-pcr genotyping of some campylobacter jejuni isolates of chicken and human origin in egypt. | the public health importance of the genus campylobacter is attributed to several species causing diarrhea in consumers. poultry and their meat are considered the most important sources of human campylobacteriosis. in this study, 287 samples from chicken (131 cloacal swabs, 39 chicken skin, 78 chicken meat, and 39 cecal parts) obtained from retail outlets as well as 246 stool swabs from gastroenteritis patients were examined. a representative number of the biochemically identified campylobacter j ... | 2015 | 26579615 |
| an insight into the isolation, enumeration, and molecular detection of listeria monocytogenes in food. | listeria monocytogenes, a foodborne pathogen that can cause listeriosis through the consumption of food contaminated with this pathogen. the ability of l. monocytogenes to survive in extreme conditions and cause food contaminations have become a major concern. hence, routine microbiological food testing is necessary to prevent food contamination and outbreaks of foodborne illness. this review provides insight into the methods for cultural detection, enumeration, and molecular identification of l ... | 2015 | 26579116 |
| multiplex detection of nine food-borne pathogens by mpcr and capillary electrophoresis after using a universal pre-enrichment medium. | routine microbiological quality analyses in food samples require, in some cases, an initial incubation in pre-enrichment medium. this is necessary in order to ensure that small amounts of pathogenic strains are going to be detected. in this work, a universal pre-enrichment medium has been developed for the simultaneous growth of bacillus cereus, campylobacter jejuni, clostridium perfringens, cronobacter sakazakii, escherichia coli, enterobacteriaceae family (38 species, 27 genera), listeria mono ... | 2015 | 26579100 |
| lineage-specific methyltransferases define the methylome of the globally disseminated escherichia coli st131 clone. | escherichia coli sequence type 131 (st131) is a clone of uropathogenic e. coli that has emerged rapidly and disseminated globally in both clinical and community settings. members of the st131 lineage from across the globe have been comprehensively characterized in terms of antibiotic resistance, virulence potential, and pathogenicity, but to date nothing is known about the methylome of these important human pathogens. here we used single-molecule real-time (smrt) pacbio sequencing to determine t ... | 2015 | 26578678 |
| an overview of pathogen recognition receptors for innate immunity in dental pulp. | pathogen recognition receptors (prrs) are a class of germ line-encoded receptors that recognize pathogen-associated molecular patterns (pamps). the activation of prrs is crucial for the initiation of innate immunity, which plays a key role in first-line defense until more specific adaptive immunity is developed. prrs differ in the signaling cascades and host responses activated by their engagement and in their tissue distribution. currently identified prr families are the toll-like receptors (tl ... | 2015 | 26576076 |
| disruptions of host immunity and inflammation by giardia duodenalis: potential consequences for co-infections in the gastro-intestinal tract. | giardia duodenalis (syn. g. intestinalis, or g. lamblia) is a leading cause of waterborne diarrheal disease that infects hundreds of millions of people annually. research on giardia has greatly expanded within the last few years, and our understanding of the pathophysiology and immunology on this parasite is ever increasing. at peak infection, giardia trophozoites induce pathophysiological responses that culminate in the development of diarrheal disease. however, human data has suggested that th ... | 2015 | 26569316 |
| species shift and multidrug resistance of campylobacter from chicken and swine, china, 2008-14. | the objective of this study was to investigate the prevalence and antimicrobial resistance of campylobacter isolated from broiler chickens and swine during 2008-14. | 2016 | 26568567 |
| characterisation of worldwide helicobacter pylori strains reveals genetic conservation and essentiality of serine protease htra. | htra proteases and chaperones exhibit important roles in periplasmic protein quality control and stress responses. the genetic inactivation of htra has been described for many bacterial pathogens. however, in some cases such as the gastric pathogen helicobacter pylori, htra is secreted where it cleaves the tumour-suppressor e-cadherin interfering with gastric disease development, but the generation of htra mutants is still lacking. here, we show that the htra gene locus is highly conserved in wo ... | 2015 | 26568477 |
| characterisation of worldwide helicobacter pylori strains reveals genetic conservation and essentiality of serine protease htra. | htra proteases and chaperones exhibit important roles in periplasmic protein quality control and stress responses. the genetic inactivation of htra has been described for many bacterial pathogens. however, in some cases such as the gastric pathogen helicobacter pylori, htra is secreted where it cleaves the tumour-suppressor e-cadherin interfering with gastric disease development, but the generation of htra mutants is still lacking. here, we show that the htra gene locus is highly conserved in wo ... | 2015 | 26568477 |
| cytological findings of 140 bile samples from dogs and cats and associated clinical pathological data. | cholecystocentesis can be part of the diagnostic workup of hepatobiliary disease in small animals, but literature on cytological evaluation of bile is scant. | 2015 | 26566964 |
| cytological findings of 140 bile samples from dogs and cats and associated clinical pathological data. | cholecystocentesis can be part of the diagnostic workup of hepatobiliary disease in small animals, but literature on cytological evaluation of bile is scant. | 2015 | 26566964 |
| phenotypic characters and molecular epidemiology of campylobacter jejuni in east china. | in this study, we investigated the distribution, phenotypic and molecular typing characters of campylobacter jejuni in domestic fowl, and livestock populations in east china, to provide some reference for researches on its molecular epidemiology. a total of 1250 samples were collected from different animal sources, and c. jejuni strains were then isolated and tested for antibiotic sensitivity. antibiotics-resistance gene and pathogenic genes were detected by polymerase chain reaction. phylogenic ... | 2016 | 26565657 |
| campylobacter species isolated from poultry and humans, and their analysis using pfge in southern brazil. | campylobacteriosis is a bacterial disease transmitted to humans through ingestion of contaminated food. six hundred samples were collected, 200 from human stool samples, 200 from poultry products and 200 from poultry feces in southern brazil, and then inoculated on blood agar plates. a total of 58% of the poultry feces, 17% of the poultry meat, and 2% the of human stools tested positive for campylobacter. positive campylobacter colonies were identified as campylobacter jejuni or campylobactercol ... | 2016 | 26561789 |
| electrochemical evidence that pyranopterin redox chemistry controls the catalysis of yedy, a mononuclear mo enzyme. | a long-standing contradiction in the field of mononuclear mo enzyme research is that small-molecule chemistry on active-site mimic compounds predicts ligand participation in the electron transfer reactions, but biochemical measurements only suggest metal-centered catalytic electron transfer. with the simultaneous measurement of substrate turnover and reversible electron transfer that is provided by fourier-transformed alternating-current voltammetry, we show that escherichia coli yedy is a monon ... | 2015 | 26561582 |
| animal models to study acute and chronic intestinal inflammation in mammals. | acute and chronic inflammatory diseases of the intestine impart a significant and negative impact on the health and well-being of human and non-human mammalian animals. understanding the underlying mechanisms of inflammatory disease is mandatory to develop effective treatment and prevention strategies. as inflammatory disease etiologies are multifactorial, the use of appropriate animal models and associated metrics of disease are essential. in this regard, animal models used alone or in combinat ... | 2015 | 26561503 |
| [molecular mimicry and guillain-barré syndrome]. | one-thirds of patients develop guillain-barré syndrome subsequent to campylobacter jejuni enteritis. molecular mimicry exists between c. jejuni lipo-oligosaccharides and human peripheral nerve gangliosides gm1 and gd1a. igg antibodies against gm1 or gd1a are produced in one out of 5,000 patients with c. jejuni enteritis. the autoantibodies bind to gangliosides at the nodes of ranvier in the peripheral motor nerves and activate complement in situ. this is followed by the disappearance of the volt ... | 2015 | 26560949 |
| [clinical phenotypes in guillain-barré syndrome]. | guillain-barré syndrome can be classified into several phenotypes according to the type of predominantly disturbed nerve fibers, distribution of muscular weakness, and electrophysiological and pathological findings. although several regional variants including fisher syndrome and pharyngeal-cervical-brachial weakness were initially reported in western researchers, many labor-intensive studies by japanese researchers have significantly contributed to defining and highlighting these variants. this ... | 2015 | 26560946 |
| membrane topology and biochemical characterization of the escherichia coli baca undecaprenyl-pyrophosphate phosphatase. | several integral membrane proteins exhibiting undecaprenyl-pyrophosphate (c55-pp) phosphatase activity were previously identified in escherichia coli that belonged to two distinct protein families: the baca protein, which accounts for 75% of the c55-pp phosphatase activity detected in e. coli cell membranes, and three members of the pap2 phosphatidic acid phosphatase family, namely pgpb, ybjg and lpxt. this dephosphorylation step is required to provide the c55-p carrier lipid which plays a centr ... | 2015 | 26560897 |
| use of next generation sequencing data to develop a qpcr method for specific detection of eu-unauthorized genetically modified bacillus subtilis overproducing riboflavin. | recently, the presence of an unauthorized genetically modified (gm) bacillus subtilis bacterium overproducing vitamin b2 in a feed additive was notified by the rapid alert system for food and feed (rasff). this has demonstrated that a contamination by a gm micro-organism (gmm) may occur in feed additives and has confronted for the first time,the enforcement laboratories with this type of rasff. as no sequence information of this gmm nor any specific detection or identification method was availab ... | 2015 | 26559764 |
| antibiotic susceptibility and molecular characterization of campylobacter jejuni strain isolated from a guillain barré syndrome child. | 2016 | 26558915 | |
| high genetic similarity of ciprofloxacin-resistant campylobacter jejuni in central europe. | campylobacteriosis is the leading zoonosis in the european union with the majority of cases attributed to campylobacter jejuni. although the disease is usually self-limiting, some severe cases need to be treated with antibiotics, primarily macrolides and quinolones. however, the resistance to the latter is reaching alarming levels in most of the eu countries. to shed light on the expansion of antibiotic resistance in central europe, we have investigated genetic similarity across 178 ciprofloxaci ... | 2015 | 26557112 |
| gut microbiota and host metabolism in liver cirrhosis. | the gut microbiota has the capacity to produce a diverse range of compounds that play a major role in regulating the activity of distal organs and the liver is strategically positioned downstream of the gut. gut microbiota linked compounds such as short chain fatty acids, bile acids, choline metabolites, indole derivatives, vitamins, polyamines, lipids, neurotransmitters and neuroactive compounds, and hypothalamic-pituitary-adrenal axis hormones have many biological functions. this review focuse ... | 2015 | 26556989 |
| conserved histidine residues at the ferroxidase centre of the campylobacter jejuni dps protein are not strictly required for metal binding and oxidation. | iron is an essential micronutrient for living organisms as it is involved in a broad variety of important biological processes. however, free iron inside the cell could be potentially toxic, generating hydroxyl radicals through the fenton reaction. dps (dna-binding protein from starved cells) belongs to a subfamily of ferritins and can store iron atoms inside the dodecamer. the presence of a ferroxidase centre, composed of highly conserved residues, is a signature of this protein family. in this ... | 2016 | 26555736 |
| tetracyclines in malaria. | malaria, a parasite vector-borne disease, is one of the greatest health threats in tropical regions, despite the availability of malaria chemoprophylaxis. the emergence and rapid extension of plasmodium falciparum resistance to various anti-malarial drugs has gradually limited the number of potential malaria therapeutics available to clinicians. in this context, doxycycline, a synthetically derived tetracycline, constitutes an interesting alternative for malaria treatment and prophylaxis. doxycy ... | 2015 | 26555664 |
| abstracts. | 2015 | 26553501 | |
| abstracts. | 2015 | 26553501 | |
| the stringent response regulator dksa is required for salmonella enterica serovar typhimurium growth in minimal medium, motility, biofilm formation, and intestinal colonization. | salmonella enterica serovar typhimurium is a facultative intracellular human and animal bacterial pathogen posing a major threat to public health worldwide. salmonella pathogenicity requires complex coordination of multiple physiological and virulence pathways. dksa is a conserved gram-negative regulator that belongs to a distinct group of transcription factors that bind directly to the rna polymerase secondary channel, potentiating the effect of the signaling molecule ppgpp during a stringent r ... | 2015 | 26553464 |
| the stringent response regulator dksa is required for salmonella enterica serovar typhimurium growth in minimal medium, motility, biofilm formation, and intestinal colonization. | salmonella enterica serovar typhimurium is a facultative intracellular human and animal bacterial pathogen posing a major threat to public health worldwide. salmonella pathogenicity requires complex coordination of multiple physiological and virulence pathways. dksa is a conserved gram-negative regulator that belongs to a distinct group of transcription factors that bind directly to the rna polymerase secondary channel, potentiating the effect of the signaling molecule ppgpp during a stringent r ... | 2015 | 26553464 |
| [antimicrobial susceptibility and resistance mutations in campylobacter jejuni and c. coli isolates from human and meat sources]. | recently, there has been a marked increase in the number of reports of fluoroquinolone-resistant campylobacter jejuni and campylobacter coli. the aim of this study was to evaluate the prevalence of antimicrobial resistance and its genetic determinants in campylobacter species isolated from meat and human subjects in fukuoka prefecture, japan. between 2011 and 2013, 55 and 64 isolates were collected from meat (chicken meat and beef liver) and humans, respectively, in this prefecture. antimicrobia ... | 2015 | 26552121 |
| a hepatic cancer patient with guillain-barré syndrome during the perioperative period of partial hepatectomy: a case report. | we reported a case of hepatic cancer patient with guillain-barré syndrome during the perioperative period of partial hepatectomy in the present study. we analyzed the clinical data and described the characteristics of this patient. | 2015 | 26550403 |
| host-microbe protein interactions during bacterial infection. | interspecies protein-protein interactions are essential mediators of infection. while bacterial proteins required for host cell invasion and infection can be identified through bacterial mutant library screens, information about host target proteins and interspecies complex structures has been more difficult to acquire. using an unbiased chemical crosslinking/mass spectrometry approach, we identified interspecies protein-protein interactions in human lung epithelial cells infected with acinetoba ... | 2015 | 26548613 |
| population diversity of campylobacter jejuni in poultry and its dynamic of contamination in chicken meat. | this study aimed to analyse the diversity of the campylobacter jejuni population in broilers and to evaluate the major source of contamination in poultry meat. eight rearing cycles over one year provided samples from three different broiler farms processed at the same slaughterhouse. a total of 707 c. jejuni were isolated from cloacal swabs before slaughter and from the breast skin of carcasses after slaughter and after chilling. all suspected campylobacter colonies were identified with pcr assa ... | 2015 | 26543870 |
| operomedb: a database of condition-specific transcription units in prokaryotic genomes. | background. in prokaryotic organisms, a substantial fraction of adjacent genes are organized into operons-codirectionally organized genes in prokaryotic genomes with the presence of a common promoter and terminator. although several available operon databases provide information with varying levels of reliability, very few resources provide experimentally supported results. therefore, we believe that the biological community could benefit from having a new operon prediction database with operons ... | 2015 | 26543854 |
| complete genome sequences of campylobacter jejuni strains rm3196 (233.94) and rm3197 (308.95) isolated from patients with guillain-barré syndrome. | infections with campylobacter jejuni subsp. jejuni are a leading cause of foodborne gastroenteritis and the most prevalent infection preceding guillain-barré syndrome (gbs). this study describes the genomes of c. jejuni subsp. jejuni hs:41 strains rm3196 (233.94) and rm3197 (308.95) that were isolated from patients with gbs in cape town, south africa. | 2015 | 26543130 |
| pacbio sequencing and its applications. | single-molecule, real-time sequencing developed by pacific biosciences offers longer read lengths than the second-generation sequencing (sgs) technologies, making it well-suited for unsolved problems in genome, transcriptome, and epigenetics research. the highly-contiguous de novo assemblies using pacbio sequencing can close gaps in current reference assemblies and characterize structural variation (sv) in personal genomes. with longer reads, we can sequence through extended repetitive regions a ... | 2015 | 26542840 |
| comparison of two forms of loperamide-simeticone and a probiotic yeast (saccharomyces boulardii) in the treatment of acute diarrhoea in adults: a randomised non-inferiority clinical trial. | acute diarrhoea is a frequent health problem in both travellers and residents that has a social and economic impact. this study compared the efficacy and tolerability of two loperamide-simeticone formulations and a saccharomyces boulardii capsule as symptomatic treatment. | 2015 | 26541878 |
| control strategies against campylobacter at the poultry production level: biosecurity measures, feed additives and vaccination. | campylobacteriosis is the most prevalent bacterial foodborne gastroenteritis affecting humans in the european union, and ranks second in the united states only behind salmonellosis. in europe, there are about nine million cases of campylobacteriosis every year, making the disease a major public health issue. human cases are mainly caused by the zoonotic pathogen campylobacter jejuni. the main source of contamination is handling or consumption of poultry meat. poultry constitutes the main reservo ... | 2016 | 26541243 |
| immunization with a double-mutant (r192g/l211a) of the heat-labile enterotoxin of escherichia coli offers partial protection against campylobacter jejuni in an adult mouse intestinal colonization model. | we have previously shown that antibodies to cholera toxin (ct) reacted with the major outer membrane proteins (momps) from campylobacter jejuni strains on western blot. further, oral immunization with ct significantly protected against challenge with c. jejuni in an adult mouse colonization model of infection. ct and the heat-labile enterotoxin (lt) of enterotoxigenic escherichia coli are structurally and functionally related. lt and its mutants including the double-mutant lt (r192g/l211a) (dmlt ... | 2015 | 26540197 |
| genetic diversity and incidence of virulence-associated genes of arcobacter butzleri and arcobacter cryaerophilus isolates from pork, beef, and chicken meat in poland. | incidence of 9 virulence-associated genes and genetic diversity was determined in 79 a. butzleri and 6 a. cryaerophilus isolates from pork, beef, and chicken meat. all a. butzleri isolates harboured the tlya gene, and most of them carried ciab, mvin, plda, cadf, and cj1349 genes. ciab was found to occur with higher frequency in poultry if compared with pork (p = 0.0007), while irga was more frequent in poultry than in beef (p = 0.007). all 6 a. cryaerophilus isolates harboured the ciab gene, whi ... | 2015 | 26539546 |
| the zinc transport systems and their regulation in pathogenic fungi. | zinc is an essential micronutrient required for many enzymes that play essential roles in a cell. it was estimated that approximately 3% of the total cellular proteins are required for zinc for their functions. zinc has long been considered as one of the key players in host-pathogen interactions. the host sequesters intracellular zinc by utilizing multiple cellular zinc importers and exporters as a means of nutritional immunity. to overcome extreme zinc limitation within the host environment, pa ... | 2015 | 26539032 |
| pathogenesis of myeloproliferative neoplasms: role and mechanisms of chronic inflammation. | myeloproliferative neoplasms (mpns) are a heterogeneous group of clonal diseases characterized by the excessive and chronic production of mature cells from one or several of the myeloid lineages. recent advances in the biology of mpns have greatly facilitated their molecular diagnosis since most patients present with mutation(s) in the jak2, mpl, or calr genes. yet the roles played by these mutations in the pathogenesis and main complications of the different subtypes of mpns are not fully eluci ... | 2015 | 26538820 |
| transcriptome analysis of campylobacter jejuni polyphosphate kinase (ppk1 and ppk2) mutants. | 2015 | 26537695 | |
| quorum sensing peptides selectively penetrate the blood-brain barrier. | bacteria communicate with each other by the use of signaling molecules, a process called 'quorum sensing'. one group of quorum sensing molecules includes the oligopeptides, which are mainly produced by gram-positive bacteria. recently, these quorum sensing peptides were found to biologically influence mammalian cells, promoting i.a. metastasis of cancer cells. moreover, it was found that bacteria can influence different central nervous system related disorders as well, e.g. anxiety, depression a ... | 2015 | 26536593 |
| genotyping of campylobacter coli strains isolated in brazil suggests possible contamination amongst environmental, human, animal and food sources. | campylobacter coli and campylobacter jejuni are two of the most common causative agents of food-borne gastroenteritis in numerous countries worldwide. in brazil, campylobacteriosis is under diagnosed and under-reported, and few studies have molecularly characterized campylobacter spp. in this country. the current study genotyped 63 c. coli strains isolated from humans (n512), animals (n521), food (n510) and the environment (n520) between 1995 and 2011 in brazil. the strains were genotyped using ... | 2016 | 26531157 |