Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| the role of human herpesvirus 8 in the pathogenesis of kaposi's sarcoma. | human herpesvirus 8 is a novel gamma-herpesvirus that has been linked with all clinical types of ks through both dna and serologic studies. by electron microscopic, in situ hybridization, and in situ pcr studies, virus is found in tumor spindle cells, in normal-appearing endothelial cells within lesions, and in tumor-infiltrating leukocytes. the fact that most tumor cells are latently, as opposed to lytically, infected with hhv8 makes the possibility of treating ks patients with antiherpesviral ... | 1999 | 10643499 |
| c-src mediates mitogenic signals and associates with cytoskeletal proteins upon vascular endothelial growth factor stimulation in kaposi's sarcoma cells. | vascular endothelial growth factor (vegf) appears to be a critical cytokine modulating the growth and spread of kaposi's sarcoma (ks). furthermore, infection with the ks herpes virus results in up-regulation of vegf and triggering of vegf receptor activation. the molecular mechanisms regulating such cytokine-driven proliferation of ks cells are not well characterized. we investigated the role of src-related tyrosine kinases in vegf-mediated signaling in model ks 38 tumor cells. vegf stimulation ... | 2000 | 10640727 |
| aids-related body cavity-based lymphomas, herpesvirus-8 and hiv infection: a study of seven cases. | 1999 | 10630536 | |
| kaposi's sarcoma following malignant mesothelioma. | we report the unusual occurrence of kaposi's sarcoma following asbestos-related malignant mesothelioma, in a human deficiency virus (hiv)-negative italian man. seropositivity to human herpes virus 8 (hhv8) was documented at the time of mesothelioma diagnosis and preceded the onset of kaposi' sarcoma with a time lapse of 13 months. hhv8 dna was detected by polymerase chain reaction in lesional kaposi's sarcoma but not within mesothelioma. by immunostaining, mesothelioma cells expressed interleuki ... | 1999 | 10628804 |
| two distinct gamma-2 herpesviruses in african green monkeys: a second gamma-2 herpesvirus lineage among old world primates? | primate gamma-2 herpesviruses (rhadinoviruses) have so far been found in humans (kaposi's sarcoma-associated herpesvirus [kshv], also called human herpesvirus 8), macaques (macaca spp.) (rhesus rhadinovirus [rrv] and retroperitoneal fibromatosis herpesvirus [rfhv]), squirrel monkeys (saimiri sciureus) (herpesvirus saimiri), and spider monkeys (ateles spp.) (herpesvirus ateles). using serological screening and degenerate consensus primer pcr for the viral dna polymerase gene, we have detected seq ... | 2000 | 10627572 |
| the role of human herpesvirus-8 in the pathogenesis of multiple myeloma. | human herpesvirus-8 has been strongly implicated in the pathogenesis of ks, bcbl, and multicentric castleman's disease. evidence for its role in the pathogenesis of multiple myeloma is accumulating. human herpesvirus-8 is detectable in the nonmalignant bone marrow dendritic cells from most myeloma patients. in addition, hhv-8 is also detected in the peripheral blood of most myeloma patients. in contrast, this virus is rarely detected in close contacts of myeloma patients, healthy individuals, or ... | 1999 | 10626142 |
| the human herpesvirus 8 homolog of epstein-barr virus sm protein (ks-sm) is a posttranscriptional activator of gene expression. | homologs of the epstein-barr virus (ebv) sm protein exist in several human and nonhuman herpesviruses. structure and function differ significantly among these proteins. we have cloned and characterized the human herpesvirus 8 (hhv8) gene, ks-sm, which is homologous to the ebv sm and herpes simplex virus icp27 genes, from an hhv8-infected primary effusion lymphoma. ks-sm is shown to be a posttranscriptional activator of gene expression in cotransfection studies. ks-sm activated gene expression in ... | 2000 | 10623771 |
| gammaherpesviruses and "hit-and-run" oncogenesis. | 2000 | 10623645 | |
| p53 inhibition by the lana protein of kshv protects against cell death. | kaposi's sarcoma-associated herpesvirus (kshv), or human herpesvirus 8, has been implicated in the development of kaposi's sarcoma (ks) and several b-cell lymphoproliferative diseases. most cells in lesions derived from these malignancies are latently infected, and different viral gene products have been identified in association with lytic or latent infection by kshv. the latency-associated nuclear antigen (lana), encoded by open reading frame 73 of the kshv genome, is a highly immunogenic prot ... | 1999 | 10622254 |
| human immunodeficiency virus-seropositive individual with persistent human herpesvirus 8 infection for >11 years without development of kaposi's sarcoma. | 2000 | 10619767 | |
| kaposi sarcoma of major salivary gland origin: a clinicopathologic series of six cases. | kaposi sarcoma (ks), one of the defining tumors of acquired immune deficiency syndrome (aids), is rarely identified in the major salivary glands. to the authors' knowledge, no previous published series has evaluated the clinicopathologic aspects of this tumor in major salivary glands. | 2000 | 10618601 |
| inflammatory cytokines and the reactivation of kaposi's sarcoma-associated herpesvirus lytic replication. | kaposi's sarcoma (ks) is a complex proliferative lesion long suspected of being dependent on exogenous paracrine signaling molecules to stimulate its proliferative, angiogenic, and inflammatory components. in particular, both clinical and experimental observations have pointed to a potential role for inflammatory cytokines as permissive factors for ks development, but ks pathogenesis is also critically dependent on infection by an exogenous herpesvirus, the ks-associated herpesvirus (kshv). to e ... | 2000 | 10612656 |
| structure and function of the cc chemokine receptor (ccr) 8. | the cc chemokine receptor (ccr) 8 belongs to the seven transmembrane-spanning receptor family and functionally responds to the eukaryotic cc chemokines i-309, thymus and activation-regulated chemokine, macrophage inflammatory protein-1 beta (mip-1b) and to the products viral mip-i and viral mip-ii of the kaposi-associated herpesvirus (hhv-8). although it has not yet been fully characterised, its restricted expression to lymphoid tissues, i.e. thymus, spleen and lymph nodes, and its abundant up-r ... | 1999 | 10611408 |
| experimental transmission of kaposi's sarcoma-associated herpesvirus (kshv/hhv-8) to scid-hu thy/liv mice. | kaposi's sarcoma-associated herpesvirus (kshv/hhv-8) is a novel human lymphotropic herpesvirus linked to several human neoplasms. to date, no animal model for infection by this virus has been described. we have examined the susceptibility of c.b-17 scid/scid mice implanted with human fetal thymus and liver grafts (scid-hu thy/liv mice) to kshv infection. kshv virions were inoculated directly into the implants, and viral dna and mrna production was assayed using real-time quantitative polymerase ... | 1999 | 10601360 |
| [the genomic sequences of the human herpesvirus 8 in biological samples from hiv-positive and -negative subjects in sicily]. | 1999 | 10596444 | |
| human interleukin-6 is in vivo an autocrine growth factor for human herpesvirus-8-infected malignant b lymphocytes. | human interleukin-6 (hil-6) acts as a growth factor in several human b lymphoid cancers. as human herpesvirus-8 (hhv-8) encodes for a viral il-6 (vil-6), the viral cytokine may be responsible for several manifestations of hhv-8-related disorders. using an anti-hil-6 mab (b-e8) which does not recognize vil-6, we investigated the involvement of the human cytokine in the proliferation of hhv-8-positive primary effusion lymphoma (pel) cells. in vitro, 5/5 pel cell lines produced hil-6 (4 to 1,200 pg ... | 1999 | 10586116 |
| no evidence for a role of human herpesvirus type 8 in sarcoidosis: molecular and serological analysis. | the aim of this study was to analyse the association between human herpesvirus type 8 (hhv8) and sarcoidosis. using nested polymerase chain reaction (pcr), we tested the presence of hhv8 dna sequences in 13 skin specimens and peripheral blood mononuclear cells from eight patients suffering from sarcoidosis. we also looked for the presence of hhv8 antibodies in the sera of 28 patients with sarcoidosis using three techniques: two indirect immunofluorescence assays and an enzyme-linked immunosorben ... | 1999 | 10583053 |
| the latency-associated nuclear antigen tethers the kaposi's sarcoma-associated herpesvirus genome to host chromosomes in body cavity-based lymphoma cells. | viruses that establish latent infection must maintain their dna in the host nucleus through many cellular generations. here we identify a novel mechanism by which the gammaherpesvirus kaposi's sarcoma-associated herpesvirus (kshv) may achieve this persistence in latently infected body cavity-based lymphoma (bcbl) cells. we find that kshv genomic dna is associated with host chromosomes and colocalizes with the latency-associated nuclear antigen (lana). furthermore, a region at the left end of the ... | 1999 | 10562490 |
| latent nuclear antigen of kaposi's sarcoma-associated herpesvirus interacts with ring3, a homolog of the drosophila female sterile homeotic (fsh) gene. | kaposi's sarcoma-associated herpesvirus (kshv/hhv-8) is the likely infectious cause of kaposi's sarcoma, primary effusion lymphoma, and some cases of multicentric castleman's disease. its latent nuclear antigen (lana) is expressed in the nuclei of latently infected cells and may play a role in the persistence of episomal viral dna in dividing cells. here we report that lana interacts with ring3, a nuclear protein and member of the drosophila fsh (female sterile homeotic) family of proteins, some ... | 1999 | 10559289 |
| seroprevalence of human herpesvirus-8 (hhv-8) in countries of southeast asia compared to the usa, the caribbean and africa. | seroprevalence of hhv-8 has been studied in malaysia, india, sri lanka, thailand, trinidad, jamaica and the usa, in both healthy individuals and those infected with hiv. seroprevalence was found to be low in these countries in both the healthy and the hiv-infected populations. this correlates with the fact that hardly any aids-related kaposi's sarcoma has been reported in these countries. in contrast, the african countries of ghana, uganda and zambia showed high seroprevalences in both healthy a ... | 1999 | 10555764 |
| motility induced by human immunodeficiency virus-1 tat on kaposi's sarcoma cells requires platelet-activating factor synthesis. | in the present study, we evaluated whether motility of kaposi's sarcoma (ks) spindle cells induced by hiv-1 tat protein is dependent on the synthesis of platelet-activating factor (paf). the results obtained indicate that tat induced a dose-dependent synthesis of paf from ks cells at a concentration as low as 0.1 ng/ml. paf production started rapidly after tat stimulation, peaking at 30 minutes and declining thereafter. tat-induced cell migration was also a rapid event starting at 30 minutes. th ... | 1999 | 10550329 |
| cytokines in the evolution and treatment of aids-lymphoma. | aids-lymphoma is a heterogeneous disease that most likely results from the complex interaction of several contributing factors, including chronic antigenic activation of b lymphocytes, dysregulated cytokine and co-stimulatory networks, infection with potentially oncogenic viruses (human herpesvirus-8 [hhv-8], epstein-barr virus), and accumulation of secondary genetic mutations. cytokines are believed to play an important role in the immunologic decline that favors opportunistic infection and mal ... | 1999 | 10550017 |
| [poems syndrome (polyneuropathy, organomegaly , endocrinopathy, monoclonal gammopathy, skin changes), multicentric castleman disease, renal chromophobe carcinoma and herpes virus type 8 infection]. | 1999 | 10544776 | |
| gene expression from the orf50/k8 region of kaposi's sarcoma-associated herpesvirus. | the orf50 gene of kaposi's sarcoma (ks)-associated herpesvirus, or human herpesvirus 8 (kshv), activates viral replication and is weakly homologous to the herpesvirus family of r transactivators; therefore, the transcription and translation events from this region of kshv are key events in viral reactivation. we demonstrate that orf50 is expressed in a bicistronic message after induction of the viral lytic cycle. orf50 migrated as a series of polypeptides: the major ones as 119 and 101 kda, resp ... | 1999 | 10544116 |
| kaposi's sarcoma-associated herpesvirus-encoded g protein-coupled receptor activation of c-jun amino-terminal kinase/stress-activated protein kinase and lyn kinase is mediated by related adhesion focal tyrosine kinase/proline-rich tyrosine kinase 2. | the kaposi's sarcoma-associated herpesvirus (kshv) (also known as human herpesvirus 8) has been implicated in the pathogenesis of kaposi's sarcoma and b cell primary effusion lymphomas. kshv encodes a g protein-coupled receptor (gpcr) that acts as an oncogene and constitutively activates two protein kinases, c-jun amino-terminal kinase (jnk)/stress-activated protein kinase (sapk) and p38 mitogen-activated protein kinase. it also induces the production of vascular endothelial growth factor. these ... | 1999 | 10542211 |
| infection, immunity, and cancer. | a significant percentage of human cancers worldwide are associated with infections due to known viruses, including human papillomaviruses (cervical cancer and other skin cancers), human t-lymphotropic viruses (adult t-cell leukemias and lymphomas in endemic areas), hepatitis b virus (liver cancer), and epstein-barr virus (burkitt lymphoma and nasopharyngeal carcinoma). the fraction of human cancers attributable to infection may now need to be revised in light of the fact that new viral associati ... | 1999 | 10539899 |
| etiology of multiple myeloma: what's new. | both the tumor cells and the microenvironment of dendritic stromal cells appear to be involved in the etiology of multiple myeloma. switch translocations in myeloma tumor cells often involve oncogenes. these translocations have a clearly established role in the etiology of lymphoma and may prove to have a role in the transformation process of myeloma. dendritic stromal cells infected with human herpesvirus-8 may provide a growth and antiapoptosis advantage for myeloma bone marrow stromal cells v ... | 1999 | 10528889 |
| the spectrum of cutaneous lymphomas in hiv infection: a study of 21 cases. | we studied 21 hiv-associated lymphomas with cutaneous presentation to determine whether they showed features of primary cutaneous lymphoma arising fortuitously or whether they represented the cutaneous involvement of aids systemic lymphoma. besides rare mycosis fungoides (n = 3), which shared typical clinicopathologic lesions, nonepidermotropic large-cell lymphomas (n = 18) were predominant. they frequently presented as a solitary nodule or tumor. seven of the eight large t-cell lymphomas had a ... | 1999 | 10524521 |
| modulation of the nf-kappa b pathway by virally encoded death effector domains-containing proteins. | death effector domains (deds) have been known to mediate the recruitment of caspase 8 and its homologs to the aggregated death-inducing signaling complex (disc), consisting of the death domain (dd)-containing receptors and various signaling proteins. in addition, several viruses were recently shown to encode proteins with deds (also called flice inhibitory proteins or vflips) which have the ability of blocking cell death induced by dd-containing receptors. we provide evidence that vflips can als ... | 1999 | 10523854 |
| involvement of interleukin-10 (il-10) and viral il-6 in the spontaneous growth of kaposi's sarcoma herpesvirus-associated infected primary effusion lymphoma cells. | primary effusion lymphoma (pel) is a distinct type of lymphoma associated with kaposi's sarcoma-associated herpesvirus (kshv) infection. to determine the factors responsible for the unrestrained proliferation of pel, we have studied the growth factor requirements of the pel-derived bcbl-1 and bc-1 cell lines. both cell lines were found to be autocrine growth factor dependent and to release human interleukin-6 (il-6), viral il-6 (vil-6), and human il-10 in the culture supernatant. to establish wh ... | 1999 | 10515891 |
| molecular polymorphism of kaposi's sarcoma-associated herpesvirus (human herpesvirus 8) latent nuclear antigen: evidence for a large repertoire of viral genotypes and dual infection with different viral genotypes. | molecular polymorphism was found in kaposi's sarcoma-associated herpesvirus (kshv) latent nuclear antigen (lna), mapped to the internal repeat domain of the encoding orf73 gene, and used to develop a novel genotyping technique, kshv lna genotyping (kvnatyping). kvnatype was stable during latent and lytic viral replication in cell culture and in humans. diverse kvnatypes were identified in 43 specimens: 6 kshv cell lines and 6 kaposi's sarcoma (ks) and 4 primary effusion lymphoma (pel) tumor samp ... | 1999 | 10515805 |
| the inhibitor of death receptor signaling, flice-inhibitory protein defines a new class of tumor progression factors. | death receptor-mediated apoptosis can be modulated by several antiapoptotic proteins, such as the flice (fadd [fas-associated death domain]-like il-1beta-converting enzyme)-inhibitory proteins (flips). the flip family includes both cellular and viral members. the kaposi's sarcoma-associated herpesvirus protein (kshv)-flip is expressed by human herpesvirus 8 (hhv-8), which is associated with malignancies such as kaposi's sarcoma and certain lymphomas. in this paper, we demonstrate that kshv-flip ... | 1999 | 10510092 |
| clinical and biological characteristics of human immunodeficiency virus-infected and uninfected intravascular drug users in ho chi minh city, vietnam. | to define the medical characteristics of intravascular drug users in ho chi minh city, vietnam, we examined 280 men, of whom 235 were infected with human immunodeficiency virus (hiv), being treated in a rehabilitation center. the patients used mainly opium, often in shooting galleries (50%). the prevalence of oral candidiasis (58%) and zoster infection (20%) was high in hiv-seropositive patients, whereas oral hairy leukoplasia and kaposi's sarcoma were absent. the prevalence of acquired immunode ... | 1999 | 10497983 |
| absence of kaposi's sarcoma-associated herpesvirus dna in bacillary angiomatosis-peliosis lesions. | bartonella henselae and b. quintana induce an unusual vascular proliferative tissue response known as bacillary angiomatosis (ba) and bacillary peliosis (bp) in some human hosts. the mechanisms of bartonella-associated vascular proliferation remain unclear. although host factors probably play a role, microbial coinfection has not been ruled out. because of the vascular proliferative characteristics noted in both kaposi's sarcoma (ks) and ba and occasional colocalization of ks and ba, the possibi ... | 1999 | 10479179 |
| expression of human herpesvirus-8 (hhv-8) encoded pathogenic genes in kaposi's sarcoma (ks) primary lesions. | transcription of six different hhv-8 specific mrnas was examined in early- and late-stage ks primary lesions. expression of the latency-associated t0.7 mrna and of vp23 mrna which is a specific marker of lytic/productive infection suggested that hhv-8 is secondarily recruited into the ks lesions by productively infected monocytes, macrophages. from these cells hhv-8 is transmitted to the ks spindle cells, which are latently infected. v-bcl-2, v-mcp-1 and v-il-6 were not expressed in latently inf ... | 1999 | 10470382 |
| human herpesvirus 8 and cancer. | 1999 | 10469737 | |
| absence of human herpes virus 8 in semen from healthy danish donors. | epidemiological data indicate a sexual route of transmission of acquired immune deficiency syndrome (aids) associated kaposi's sarcoma. recently human herpes virus 8 (hhv-8) has been proposed as the aetiological agent for development of kaposi's sarcoma. further the virus has been reported in semen obtained from healthy men. in denmark strict biochemical and microbiological criteria are used in combination with an intensive interview to select semen donors. despite these strict criteria, hhv-8 m ... | 1999 | 10469694 |
| human herpes virus 8 (hhv8) serology in allogeneic bone marrow transplant recipients. | human herpes virus 8 (hhv8) may be sexually transmitted, but transmission via blood cells has not yet been excluded. we used a modified immunofluorescence assay to detect ab to hhv8 latency-associated nuclear ag in sera of 200 allogeneic bmt recipients and their related donors. in control subjects, ab were found in 85% of patients with aids-related kaposi sarcoma (n = 52), 34% of hiv-1 infected subjects without kaposi sarcoma (n = 56) and 9. 5% of blood donors (n = 42). among bmt donors, 14.5% w ... | 1999 | 10467321 |
| transcriptional activation by the human herpesvirus-8-encoded interferon regulatory factor. | human herpesvirus-8 (hhv-8), a gammaherpesvirus that is thought to be the viral aetiologic agent of kaposi's sarcoma and primary effusion lymphoma, encodes a homologue to cellular interferon regulatory factors (irfs). the hhv-8 irf homologue (virf; orf k9) has previously been shown to inhibit gene induction by interferons and irf-1 and to transform nih3t3 cells or rat-1 cells. additionally, expression of antisense to virf in bcbl-1 cells results in the repression of certain hhv-8 genes, suggesti ... | 1999 | 10466820 |
| hhv-8 infection and multiple myeloma. | human herpes virus 8 (hhv-8) also known as kaposi's sarcoma-associated herpes virus has been strongly implicated in the pathogenesis of kaposi's sarcoma (ks), primary effusion lymphoma, and multicentric castleman disease. recently, this gamma-herpes virus was also found in the nonmalignant bone marrow dendritic cells of the majority of myeloma patients. in addition, hhv-8 is also detectable in the peripheral blood of most myeloma patients. in contrast, this virus is rarely detected in close cont ... | 1999 | 10449181 |
| unraveling immunity to gamma-herpesviruses: a new model for understanding the role of immunity in chronic virus infection. | murine gamma-herpesvirus 68 (gammahv68) infection is a new model for understanding how immunity and chronic gamma-herpesvirus infection inter-relate. gammahv68 is closely related to the human epstein-barr virus and kaposi's sarcoma herpesvirus and is associated with tumors, vasculitis of the great elastic arteries and splenic fibrosis. advances in the past year have provided an even stronger foundation for believing that gammahv68 infection of normal and mutant mice will become the pre-eminent a ... | 1999 | 10448140 |
| inflammatory cytokines synergize with the hiv-1 tat protein to promote angiogenesis and kaposi's sarcoma via induction of basic fibroblast growth factor and the alpha v beta 3 integrin. | the tat protein of hiv-1, a transactivator of viral gene expression, is released by acutely infected t cells and, in this form, exerts angiogenic activities. these have linked the protein to the pathogenesis of kaposi's sarcoma (ks), a vascular tumor frequent and aggressive in hiv-1-infected individuals (aids-ks). in this study, we show that a combination of the same inflammatory cytokines increased in ks lesions, namely il-1 beta, tnf-alpha, and ifn-gamma, synergizes with tat to promote in nude ... | 1999 | 10438928 |
| functional analysis of human herpesvirus 8-encoded viral interferon regulatory factor 1 and its association with cellular interferon regulatory factors and p300. | human herpesvirus 8/kaposi sarcoma-associated virus (hhv-8/kshv) contains, in addition to genes required for viral replication, a unique set of nonstructural genes which may be part of viral mimicry and contribute to viral replication and pathogenesis in vivo. among these, hhv-8 encodes four open reading frames (orfs) that showed homology to the transcription factors of the interferon regulatory factor (irf) family. the orf k9, viral irf 1 (virf-1), has been cloned, and it was shown that, when o ... | 1999 | 10438822 |
| [human herpes virus 8 and kaposi's sarcoma]. | 1999 | 10436595 | |
| oncological complications of human immunodeficiency virus disease and hematologic consequences of their treatment. | despite advances in antiretroviral therapy and in the treatment and prevention of opportunistic infections, oncological and consequent hematologic complications of human immunodeficiency virus (hiv) infection continue to occur and are of significant clinical importance. virus-related tumors (e.g., kaposi's sarcoma, induced by human herpesvirus 8; non-hodgkin's lymphoma, linked to epstein-barr virus; and anogenital tumors, linked to human papillomavirus) are frequent in patients with hiv-induced ... | 1999 | 10433562 |
| overview of the epidemiology of malignancy in immune deficiency. | immune-suppressed populations experience higher rates of cancer than expected. the most common malignancies are non-hodgkin's lymphoma (nhl) in those with hiv infection, in organ transplant recipients, and in those with primary immune deficiencies; kaposi's sarcoma (ks) in those with hiv infection; and nonmelanoma skin cancer (sc) in transplant patients. these cancers are associated with infection with the epstein-barr virus (ebv) in nhl, human herpesvirus type 8 (hhv-8) in ks, and the human pap ... | 1999 | 10430211 |
| establishing and characterizing a cd30-positive cell line harboring hhv-8 from a primary effusion lymphoma. | primary effusion lymphoma (pel, or body-cavity-based lymphoma [bcbl]) is a new subtype of non-hodgkin's lymphoma in which tumor cells locate in the body cavity exclusively. pel/bcbl is widely accepted as one of the neoplastic complications of aids, associated mostly with human herpesvirus 8 (hhv-8/kaposi's sarcoma-associated herpesvirus [kshv]) and epstein-barr virus (ebv). we established and characterized a pel cell line named ty-1 from a 47-year-old patient with aids. ty-1 exhibits indetermina ... | 1999 | 10421407 |
| absence of a link between human herpesvirus 8 and pemphigus. | 1999 | 10417537 | |
| dominant human herpesvirus type 8 rna transcripts in classical and aids-related kaposi's sarcoma. | skin biopsy sections of kaposi's sarcoma (ks) from 25 patients (5 aids-related, 20 classical cases) were histologically staged and hybridized in situ with oligonucleotide probes for constitutively transcribed human herpesvirus 8 (hhv-8) mrna t0.7 and t1.1 using a colourimetric technique. t1.1 increases during experimental induction of the viral lytic phase in the hhv-8-infected lymphocytes of primary effusion lymphoma and its colourimetric detection in ks cells presumably corresponds to virion p ... | 1999 | 10398125 |
| cancer (or lack thereof) and viruses. | 1999 | 10390633 | |
| the human herpesvirus-type 8 is not involved in malignant melanoma. | malignant melanomas were supposed to harbour the human herpesvirus-type 8 (hhv-8) genome, as melanoma cells were reported to express interleukin-6 and a homologue of interleukin-6 was found in the hhv-8 genome. we therefore investigated 33 primary malignant melanomas by polymerase chain reaction, but could not find this tumorigenic gamma-herpesvirus in any tumour. | 1999 | 10389979 |
| an unusual cluster of cases of castleman's disease during highly active antiretroviral therapy for aids. | 1999 | 10375310 | |
| [factors associated with survival in kaposi's sarcoma in patients infected with the human immunodeficiency virus. clinical epidemiological group for aids in aquitaine]. | to study behavioral risk factors of kaposi's sarcoma (ks) among hiv infected homosexuals in bordeaux, southwest france. | 1999 | 10367298 |
| identification and rapid quantification of early- and late-lytic human herpesvirus 8 infection in single cells by flow cytometric analysis: characterization of antiherpesvirus agents. | human herpesvirus 8 (hhv-8) infection is associated with kaposi's sarcoma, primary effusion lymphoma (pel), and multicentric castleman's disease. in this study, we used monoclonal antibodies (mabs) directed against hhv-8 lytic cycle-associated proteins encoded by open reading frame (orf) 59 (nuclear pf-8 protein) and orf k8.1 (viral envelope glycoprotein k8.1 [gpk8.1]) to investigate hhv-8 lytic infection in single cells. lytically infected cells were labeled with mabs, stained with fluorescentl ... | 1999 | 10364341 |
| identification of the immediate-early transcripts of kaposi's sarcoma-associated herpesvirus. | in the immediate-early phase of reactivation or primary infection, herpesviruses express a small number of genes without requiring prior viral protein synthesis. immediate-early genes usually encode regulatory proteins critical for the viral life cycle. kaposi's sarcoma-associated herpesvirus (kshv) gene transcription in the immediate-early stage of viral reactivation was examined by using a chemical induction combined with a gene expression screening method. rna transcripts from at least four k ... | 1999 | 10364304 |
| reactivation and persistence of human herpesvirus-8 infection in b cells and monocytes by th-1 cytokines increased in kaposi's sarcoma. | patients with kaposi's sarcoma (ks) have a human herpesvirus-8 (hhv-8) load higher than patients without ks and present a cd8(+) t-cell activation with production of th1-type cytokines both in tissues and peripheral blood mononuclear cells (pbmc). because in tissues of ks patients detection of inflammatory cytokines (ic) can precede detection of hhv-8 dna and because signs of immunoactivation and/or dysregulation can precede ks development, we investigated the effect of ic on hhv-8 infection. to ... | 1999 | 10361101 |
| viral encoded cyclins. | cyclins are known effectors of cellular proliferation. while originally considered as the product of cellular genes, it is now clear that representatives of this class of proteins can be encoded by certain viruses. one of these viruses is hhv-8, a gamma herpesvirus implicated as a causative agent of kaposi's sarcoma and lymphomas in humans. the significance of the virally encoded cyclin proteins in viral propagation is as yet unclear. however, the fact that deregulation of cellular cyclin expres ... | 1999 | 10343073 |
| the descent of human herpesvirus 8. | analysis of conserved herpesvirus genes shows that human herpesvirus 8 (hhv8) belongs to a clade of primate herpesviruses within the gamma2 sublineage. overall, this clade has developed by cospeciation with host species. seventeen genes of hhv8 and its nearest relatives lack simple homologues in other herpesviruses, and eight of these have known mammalian homologues, presumed to be the sources of the hhv8 versions. comparative analyses yield further insights into the source or time of acquisitio ... | 1999 | 10343071 |
| the role of kaposi's sarcoma-associated herpesvirus (kshv/hhv-8) in lymphoproliferative diseases. | the kaposi's sarcoma-associated herpesvirus (kshv), also called human herpesvirus 8 (hhv-8), has been found to be present in a limited subset of lymphoproliferative disorders. among these are the primary effusion lymphomas, formerly designated body cavity-based lymphomas, a rare type of malignant lymphoma which possesses an unusual set of clinical and biologic features, suggesting that they represent a distinct disease entity. this virus is also present in a large proportion of cases of multicen ... | 1999 | 10343068 |
| human herpes virus-8 and other risk factors for kaposi's sarcoma in kidney transplant recipients. groupe cooperatif de transplantation d' ile de france (gcif). | the exact reasons for the high incidence of kaposi's sarcoma (ks) after kidney transplantation are still unknown. immunosuppression is classically considered as the main risk factor, but the relative risk contributed by the patient's geographic origin and by human herpes virus (hhv)-8 infection still has to be determined. | 1999 | 10342315 |
| kaposi's sarcoma-associated herpesvirus infection in the lung in multicentric castleman's disease. | a 32-year-old female was admitted for evaluation of multiple infiltrates on a chest radiograph. a diagnosis of multicentric castleman's disease was made on the basis of typical clinical manifestations. transbronchial lung biopsy (tblb) revealed histological findings reported in lymphocytic interstitial pneumonia. both the polymerase chain reaction and in situ hybridization with a probe specific for kaposi's sarcoma-associated herpesvirus (kshv) sequences demonstrated the presence of kshv in the ... | 1999 | 10337942 |
| paclitaxel in the treatment of human immunodeficiency virus 1-associated kaposi's sarcoma--drug-drug interactions with protease inhibitors and a nonnucleoside reverse transcriptase inhibitor: a case report study. | to describe the pharmacokinetics of paclitaxel and to investigate the interaction potential with protease inhibitors (indinavir, ritonavir, saquinavir) and the nonnucleoside reverse transcriptase inhibitor nevirapine, for which strong theoretical indications for clinically relevant drug interactions exist. | 1999 | 10321513 |
| kaposi's sarcoma-associated herpesvirus and multiple myeloma: lack of criteria for causality. | 1999 | 10233868 | |
| relationship of hhv8 replication and kaposi's sarcoma after solid organ transplantation. | hhv8 dna sequences have recently been isolated from all types of kaposi's sarcomas, and its association in the etiopathogenesis of this tumor has been established. however, little is known about the regulation of hhv8 replication in immunocompromised patients seropositive for this virus, and its impact on the development of kaposi's sarcoma (ks). through the study of a heart transplant patient who developed ks and in whom peripheral blood lymphocytes (pbls) had been prospectively collected befor ... | 1999 | 10232577 |
| human herpesvirus-8 (hhv-8) gene expression in kaposi's sarcoma (ks) primary lesions: an in situ hybridization study. | 1999 | 10232382 | |
| detection of human herpesvirus 8 in cervicovaginal secretions and seroprevalence in human immunodeficiency virus type 1-seropositive and -seronegative women. | epidemiologic studies suggest that human herpesvirus 8 (hhv-8) may be sexually transmitted. to study the potential for hhv-8 transmission through cervicovaginal (cv) secretions, the presence of hhv-8 dna was investigated by nested polymerase chain reaction in the cellular fraction of cv secretions from 36 human immunodeficiency virus type 1 (hiv-1)-seropositive and 29 hiv-1-seronegative women. the same patients were tested for antibodies to two defined hhv-8 antigens (latency-associated nuclear ... | 1999 | 10228077 |
| identification and characterization of human herpesvirus 8 open reading frame k9 viral interferon regulatory factor by a monoclonal antibody. | to identify and characterize an open reading frame (orf) in association with its mrna and a coding protein recognized by anti-human herpesvirus 8 (hhv-8) monoclonal antibody b291. | 1999 | 10225208 |
| pleural fluid findings in patients with the acquired immunodeficiency syndrome: correlation with concomitant pulmonary disease. | we sought to define the spectrum of conditions associated with pleural effusion (pe) in patients with the acquired immunodeficiency syndrome (aids) who submitted to diagnostic thoracentesis. | 1999 | 10219358 |
| absence of human herpesvirus-8 dna in angiosarcoma. | 1999 | 10215793 | |
| localization of human herpesvirus type 8 (hhv-8) in the kaposi's sarcoma tissues and the semen specimens of hiv-1 infected and uninfected individuals by utilizing in situ polymerase chain reaction. | kaposi's sarcoma (ks) is a form of skin cancer, most commonly found in individuals suffering from acquired immunodeficiency syndrome, or aids. however, before the worldwide infection of human immunodeficiency virus (hiv), the rare occurrence of ks was confined to two distinct groups of individuals. in the western world, the classical form of ks was often found in older men (60-70 years of age) from the mediterranean area. another form called endemic ks, was found in equatorial africa. currently, ... | 1998 | 10213307 |
| human herpesvirus 8 (hhv-8) in familial kaposi's sarcoma. | human herpesvirus 8 (hhv-8) has been detected in various epidemiological forms of kaposi's sarcoma (ks). since familial ks cases are exceedingly rare and the occurrence of familial ks in siblings has thought to depend rather on genetic factors than on a viral factor, familial ks has not been investigated for the presence of hhv-8. to investigate whether hhv-8 is present also in this rare form of ks, we examined tumor biopsies of 2 siblings with familial ks for the presence of hhv-8 specific dna ... | 1999 | 10206723 |
| cellular tropism and viral interleukin-6 expression distinguish human herpesvirus 8 involvement in kaposi's sarcoma, primary effusion lymphoma, and multicentric castleman's disease. | human herpesvirus 8 (hhv-8) infection has been implicated in the etiology of kaposi's sarcoma (ks), primary effusion lymphoma (pel), and multicentric castleman's disease (mcd), three diseases that frequently develop in immunocompromised, human immunodeficiency virus-positive individuals. one hypothesis that would account for different pathological manifestations of infection by the same virus is that viral genes are differentially expressed in heterogeneous cell types. to test this hypothesis, w ... | 1999 | 10196314 |
| kaposi's sarcoma in the gambia, west africa is less frequent in human immunodeficiency virus type 2 than in human immunodeficiency virus type 1 infection despite a high prevalence of human herpesvirus 8. | to investigate the distribution of kaposi's sarcoma (ks) cases in patients with human immunodeficiency virus types 1 and 2 (hiv-1 and hiv-2) infection in the gambia; to document the prevalence of human herpesvirus 8 (hhv-8) infection in various population groups in the gambia. | 1998 | 10195242 |
| aids-related kaposi's sarcoma patients with visceral manifestations. response to human chorionic gonadotropin preparations. | in vitro cell culture studies and a murine model for human kaposi's sarcoma (ks) have shown that human chorionic gonadotropin (hcg)-associated factor (haf) isolated from commercial hcg preparations has antiproliferative and cell killing effects on neoplastic ks cells, without toxic effects on normal endothelial cells and lymphocytes. these findings prompted preliminary study of hcg preparations for patients with early-stage ks with skin lesions only and no known visceral involvement. complete or ... | 1998 | 10195236 |
| hhv-8 and multiple myeloma. | recently, a new member of the gamma-herpesvirus family, human herpesvirus 8 (hhv-8), was identified in a case of kaposi's sarcoma. this virus has also been found in the nonmalignant dendritic cells of the bone marrow from myeloma patients. in addition, hhv-8 is also detectable in the peripheral blood of most patients although its absence suggests earlier stage disease. by contrast, this virus is undetectable in the blood of family members and sexual partners of myeloma patients. sequencing of hh ... | 1999 | 10192878 |
| comparison of human sera reactivities in immunoblots with recombinant human herpesvirus (hhv)-8 proteins associated with the latent (orf73) and lytic (orfs 65, k8.1a, and k8.1b) replicative cycles and in immunofluorescence assays with hhv-8-infected bcbl-1 cells. | the development of reliable, sensitive, and specific serological methods for the detection of human herpesvirus-8 (hhv-8) antibodies is critical for a thorough understanding of hhv-8 prevalence and pathogenesis. to evaluate the potential usefulness of hhv-8 proteins in measuring the responses against both latent and lytic antigens, we selected 1 latent [open reading frame (orf) 73] antigen and 3 hhv-8 lytic antigens (orfs 65, k8.1a, and k8.1b) previously identified as immunogenic [virology (1998 ... | 1999 | 10191203 |
| human herpesvirus 8 and renal transplantation. | 1999 | 10189288 | |
| distinct serum cytokines in aids-related skin diseases. | to determine whether common skin diseases associated with human immunodeficiency virus (hiv) were distinguishable based on the pattern of serum cytokine expression, we studied patients with psoriasis, pruritus, and kaposi's sarcoma (ks) for levels of tumor necrosis factor (tnf)-alpha, interferon-gamma (ifn-y), interleukin (il)-10, and il-4. thirty-two hiv-positive (hiv+) patients including 8 with ks, 11 with psoriasis, and 13 with pruritus along with 16 hiv-negative subjects with psoriasis were ... | 1998 | 10189188 |
| seroprevalence of human immunodeficiency virus type 1 infection in childhood malignancy in zimbabwe. | to determine the association between malignancy and the human immunodeficiency virus type 1 (hiv-1) infection in children. | 1998 | 10101432 |
| hydrocortisone activation of human herpesvirus 8 viral dna replication and gene expression in vitro. | patients undergoing chronic steroid therapy for organ transplantation are at increased risk for development of human herpes virus 8(hhv-8)-associated kaposi's sarcoma (ks). it has also been reported that following steroid withdrawal, ks lesions often undergo partial or complete regression. | 1999 | 10096517 |
| viruses and human cancer. | several kinds of viruses cause cancer in humans, accounting for 10-20% of cancer worldwide. human cancer viruses include human papillomavirus, hepatitis b virus, epstein-barr virus, human t cell lymphotropic virus, and kaposi's sarcoma-associated herpesvirus. cancer viruses alter the machinery of the infected cell to promote their own survival and growth. in the process, they interfere with normal cellular control mechanisms, leading to abnormal growth, genetic alterations and malignancy. studie ... | 1998 | 10093516 |
| ebv gene expression not altered in rheumatoid synovia despite the presence of ebv antigen-specific t cell clones. | t cells infiltrating the rheumatoid arthritis (ra) joint are oligoclonal, implicating an ag-driven process, but the putative joint-specific ags remain elusive. here we examine expression of selected ebv genes in ra synovia and find no abnormal expression in ra. dna of cmv and ebv was detectable by pcr in the synovial tissue of ra. rna of several latent and lytic ebv genes was also detectable. however, there were no differences in ebv gene expression in synovial tissues or peripheral blood when c ... | 1999 | 10092832 |
| kaposi's sarcoma-associated herpesvirus (human herpesvirus-8) encodes a homologue of the epstein-barr virus bzip protein eb1. | analysis of the recently completed genomic sequence of kaposi's sarcoma-associated herpesvirus (human herpesvirus-8) revealed that orf 50 encodes a protein with homology to the epstein-barr virus (ebv) transcription factor r. in this report, we show that orf k8, contiguous to orf 50, is interrupted by two introns and that the spliced rna is translated into a bzip protein that has homology to the ebv transcription factor eb1. the newly characterized k8 protein forms homodimers but does not hetero ... | 1999 | 10091993 |
| [hhv8 infection (kaposi's sarcoma)]. | 1999 | 10088335 | |
| kaposi's sarcoma: breeding ground of herpesviridae - a tour de force over viral evolution (review). | after reviewing the molecular biological basis of prominent theories for the integration of viruses into the earliest forms of living matter, an account is given on the immunoevasive strategies viruses have had to acquire in order to secure their existence against the most sophisticated anti-viral defensive mechanisms evolving in their hosts. herpes-viridae and kaposi's sarcoma illustrate the complexity of host-virus relationship. in following the evolutionary steps of simians and hominoids to h ... | 1999 | 10087309 |
| transplantation-associated kaposi's sarcoma: herpesvirus 8 transmission through renal allografts. | 1999 | 10083406 | |
| kaposi's sarcoma in women: a clinicopathologic study. | although all epidemiologic subsets of kaposi's sarcoma (ks) (i.e., sporadic, endemic, epidemic, and iatrogenic) have an association with human herpes virus 8 (hhv8), these subsets occur in patient populations with distinctive clinical features. to a variable degree men outnumber women in all subsets. | 1999 | 10082593 |
| human herpesvirus 8 seroprevalence and evaluation of nonsexual transmission routes by detection of dna in clinical specimens from human immunodeficiency virus-seronegative patients from central and southern italy, with and without kaposi's sarcoma. | in order to investigate the seroprevalence of human herpesvirus 8 (hhv-8) infection in central and southern italy, sera from human immunodeficiency virus (hiv)-seronegative subjects, with and without kaposi's sarcoma (ks), were analyzed by immunofluorescence assay, using bc-3, a cell line latently infected with hhv-8. high titers of antibody against hhv-8 lytic and latent antigens were detected in all 50 ks patients studied, while in 50 hiv-seronegative subjects without ks, 32 (64%) were found p ... | 1999 | 10074540 |
| sequence and genomic analysis of a rhesus macaque rhadinovirus with similarity to kaposi's sarcoma-associated herpesvirus/human herpesvirus 8. | we have sequenced the long unique region (lur) and characterized the terminal repeats of the genome of a rhesus rhadinovirus (rrv), strain 17577. the lur as sequenced is 131,364 bp in length, with a g+c content of 52.2% and a cpg ratio of 1.11. the genome codes for 79 open reading frames (orfs), with 67 of these orfs similar to genes found in both kaposi's sarcoma-associated herpesvirus (kshv) (formal name, human herpesvirus 8) and herpesvirus saimiri. eight of the 12 unique genes show similarit ... | 1999 | 10074154 |
| human herpesvirus-8 (hhv-8) associated with small non-cleaved cell lymphoma in a child with aids. | the association of human herpesvirus-8 (hhv-8) with a small non-cleaved cell lymphoma is described in a child with the acquired immunodeficiency syndrome (aids) who developed a malignant pleural effusion and radiologic evidence of multiple solid tumors. hhv-8 dna and epstein-barr virus dna were identified in pleural fluid cells by polymerase chain reaction (pcr) amplification. the serum antibody titer against lytic hhv-8 proteins was 1:640; antibodies to latent hhv-8 proteins were not detected. ... | 1999 | 10072113 |
| human immunodeficiency virus-associated pericardial effusion: report of 40 cases and review of the literature. | human immunodeficiency virus (hiv)-associated pericardial effusion is common. we present its clinical features, cause, and prognosis on the basis of a review of 40 cases at a single public hospital. | 1999 | 10047635 |
| pericardial involvement in human immunodeficiency virus infection. | previous studies have showed that the pericardium is frequently involved in hiv infection. however, the characteristics and etiology of the pericardial abnormalities that have been found remained poorly defined. we analyzed the features of pericardial involvement in these patients and investigated the clinical variables associated with moderate and severe effusions. | 1999 | 10027441 |
| absence of kaposi's sarcoma--associated herpesvirus dna sequences in multiple myeloma. | 1999 | 10025982 | |
| risk factors for rebleeding and mortality from acute upper gastrointestinal hemorrhage in human immunodeficiency virus infection. | in the general population, acute upper gastrointestinal hemorrhage (ugih) is a common problem that results in significant morbidity and mortality. the aim of this study was to determine the etiology, clinical outcome, and risk factors for rebleeding and mortality in a large cohort of human immunodeficiency virus (hiv)-infected patients with acute ugih. | 1999 | 10022629 |
| kaposi's sarcoma-associated herpesvirus encodes a bzip protein with homology to bzlf1 of epstein-barr virus. | kaposi's sarcoma-associated herpesvirus (kshv) is a recently discovered human gamma herpesvirus strongly implicated in aids-related neoplasms. we report here the identification in the kshv genome of a gene for a protein designated k-bzip and belonging to the basic-leucine zipper (bzip) family of transcription factors. k-bzip shows significant homology to bzlf1, which plays a key role in the replication and reactivation of epstein-barr virus. k-bzip is a homodimerizing protein of 237 amino acids ... | 1999 | 9971770 |
| classic kaposi's sarcoma as a second primary neoplasm. | although the incidence of classic kaposi's sarcoma (cks) has been investigated, its occurrence following a primary neoplasm and its association with this first neoplasm need to be determined. we analyzed a series of 124 patients with a secondary cks (8.4% of a total of 1485 incident cases) which occurred between 1961 and 1992 in the jewish israeli population. data on first neoplasms and subsequent kaposi's sarcoma were retrieved from the israel cancer registry. acquired-immune-deficiency-syndrom ... | 1999 | 9935196 |
| modulation of p27(kip1) levels by the cyclin encoded by kaposi's sarcoma-associated herpesvirus. | dna tumour viruses have evolved a number of mechanisms by which they deregulate normal cellular growth control. we have recently described the properties of a cyclin encoded by human herpesvirus 8 (also known as kaposi's sarcoma-associated herpesvirus) which is able to resist the actions of p16(ink4a), p21(cip1) and p27(kip1) cdk inhibitors. here we investigate the mechanism involved in the subversion of a g1 blockade imposed by overexpression of p27(kip1). we demonstrate that binding of k cycli ... | 1999 | 9927425 |
| transfusion of the hiv-seropositive patient: immunomodulation, viral reactivation, and limiting exposure to ebv (hhv-4), cmv (hhv-5), and hhv-6, 7, and 8. | 1999 | 9924760 | |
| epidemiology, etiology and pathogenesis, clinical presentations and therapeutic approaches in kaposi's sarcoma: 15-year lessons from aids. | kaposi's sarcoma (ks) is a leading cause of morbidity among 25-30% of aids patients and is by far the most common aids-associated malignancy. since the beginning of the 1990s the incidence of ks continuously declined, but it was the introduction of protease inhibitors that markedly reinforced this phenomenon. in our experience, the annual incidence was 59/1,000 patient-years in 1984 vs 21.2/1,000 patient-years in 1990, 12.5/1,000 patient-years in 1996, with a drop to 3.2/1,000 patient-years in 1 ... | 1998 | 9921413 |
| detection and sequence diversity of kaposi's sarcoma-associated herpesvirus (kshv)/human herpesvirus 8 (hhv-8) dna. | detection of kaposi's sarcoma (ks)-associated herpesvirus (kshv)/human herpesvirus (hhv-8) has been reported frequently in patients with ks associated with acquired immunodeficiency syndrome (aids). we examined the presence of the kshv sequence in 8 hiv-positive patients comprising 5 with ks, 2 with syphilis, 1 with prurigo, and 2 hiv-negative patients with angiosarcoma. using the polymerase chain reaction, we observed amplification of a dna fragment of the expected size in 4 patients with ks. s ... | 1999 | 9920979 |