Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| identification of diagnostic proteins in mycobacterium avium subspecies paratuberculosis by a whole genome analysis approach. | mycobacterium avium subspecies paratuberculosis (m. paratuberculosis) is an economically significant veterinary pathogen that causes johne's disease in cattle and sheep. there is a critical need for improved diagnostic tests to detect m. paratuberculosis infection in these animals. as with many other animal diseases, efforts need to be concentrated on the development of simple, rapid, noninvasive tests that can be performed by veterinarians or animal producers without expensive laboratory equipm ... | 2006 | 16957356 |
| transgenic cows that produce recombinant human lactoferrin in milk are not protected from experimental escherichia coli intramammary infection. | this is the first study describing an experimental mastitis model using transgenic cows expressing recombinant human lactoferrin (rhlf) in their milk. the aim of the study was to investigate the concentrations in milk and protective effects of bovine and recombinant human lactoferrin in experimental escherichia coli mastitis. experimental intramammary infection was induced in one udder quarter of seven first-lactating rhlf-transgenic cows and six normal cows, using an e. coli strain isolated fro ... | 2006 | 16954396 |
| decomposition of biological macromolecules by plasma generated with helium and oxygen. | in this study, we attempted to characterize the biomolecular effects of an atmospheric pressure cold plasma (apcp) system which utilizes helium/oxygen (he/o(2)). apcp using he/o(2) generates a low level of uv while generating reactive oxygen radicals which probably serve as the primary factor in sterilization; these reactive oxygen radicals have the advantage of being capable to access the interiors of the structures of microbial cells. the damaging effects of plasma exposure on polypeptides, dn ... | 2006 | 16953185 |
| a counterintuitive mg2+-dependent and modification-assisted functional folding of mitochondrial trnas. | mitochondrial trnas (mtrnas) often lack domains and posttranscriptional modifications that are found in cytoplasmic trnas. these structural and chemical elements normally stabilize the folding of cytoplasmic trnas into canonical structures that are competent for aminoacylation and translation. for example, the dihydrouridine (d) stem and loop domain is involved in the tertiary structure of cytoplasmic trnas through hydrogen bonds and a mg2+ bridge to the ribothymidine (t) stem and loop domain. t ... | 2006 | 16949614 |
| use of routine beef carcase escherichia coli monitoring data to investigate the relationship between hygiene status of incoming stock and processing efficacy. | in australian export-registered abattoirs microbiological monitoring is carried out within the e. coli and salmonella monitoring (esam) program. during the calendar year 2003, the esam database indicated a national prevalence of escherichia coli of around 3.0% for steers/heifers and 7.1% for cows/bulls. an investigation was carried out to attempt to elucidate why some establishments had e. coli prevalence markedly higher or markedly lower than the national average. the investigation was based on ... | 2006 | 16949171 |
| expression and properties of recombinant ovine uterine serpin. | ovine uterine serpin (ovus) is produced in the uterus of sheep under the influence of progesterone. it weakly inhibits pepsin and reduces proliferation of lymphocytes, tumor cell lines, and preimplantation embryos. when purified from uterine fluid, the concentration required for its antiproliferative effect in vitro is approximately 0.25-1 mg/ml. here we show that recombinant (r) ovus is a more potent regulator of cell proliferation than native (n) ovus purified from uterine fluid. to produce ro ... | 2006 | 16946400 |
| effect of lactoferrin on enteroaggregative e. coli (eaec). | we previously demonstrated that lactoferrin inhibits adherence of enteropathogenic escherichia coli to hep-2 cells and decreases invasiveness of shigella flexneri in hela cells by disruption of the type iii secretory system (ttss) of both enteropathogens. to determine whether these effects were specific to the ttss, we assessed the activity of bovine lactoferrin on enteroaggregative e. coli (eaec), enteropathogens whose virulence is not ttss dependent. bovine lactoferrin at a concentration of 1. ... | 2006 | 16936809 |
| escherichia coli o157: burger bug or environmental pathogen? | the three main pathways of escherichia coli o157 infection are foodborne, environmental (including direct contact with animals and their faeces and contaminated water supplies) or person to person contact. the disease is often nicknamed the 'burger bug' but it appears that environmental risk factors may be more important. in this study we use four techniques (outbreak analysis, case-control studies, disease mapping and quantitative microbial risk assessment (qmra)) to determine whether burgers o ... | 2006 | 16934897 |
| factors affecting the relationship between caeruloplasmin activity and plasma copper concentration in cattle. | 2006 | 16921015 | |
| the cytokine response of circulating peripheral blood mononuclear cells is changed after intravenous injection of lipopolysaccharide in cattle. | the aim of the study was to investigate lipopolysaccharide (lps)-induced short and long term changes in capacity for intracellular cytokine-production of bovine circulating peripheral blood mononuclear cells (pbmcs). eight dairy cows each received three intravenous injections of escherichia coli lps (10, 100 and 1000ng/kg, consecutively) at 3week intervals. intracellular cytokine production was determined by flow cytometry in pbmcs obtained 0, 2, 6 and 24h after each lps challenge. after lps adm ... | 2007 | 16914332 |
| escherichia coli isolates' serotypes, genotypes, and virulence genes and clinical coliform mastitis severity. | dairy cattle with clinical mastitis caused by escherichia coli exhibit a wide range of disease severity, from mild, with only local inflammatory changes of the mammary gland, to severe, with significant systemic derangement. the present study was designed to examine the relationship between serotype and virulence genes of e. coli mastitis isolates, different levels of systemic disease severity, and farm from which the e. coli strain was obtained. one hundred twenty-three e. coli milk isolates we ... | 2006 | 16899673 |
| bacterial production of biologically active canine interleukin-1beta by seamless sumo tagging and removal. | interleukin 1beta (il-1beta) is a potent stimulator of extracellular matrix degradation in models of osteoarthritis (oa). in contrast to bovine explant models which effectively respond to recombinant human il-1beta, canine models are relatively refractory to human il-1beta stimulation. canine il-1beta cdna was cloned in order to produce a fully potent species matched preparation of il-1beta for use specifically in canine models of oa. established methods for the production of various orthologous ... | 2006 | 16893658 |
| ribotyping of fusobacterium necrophorum strains isolated from bovine and ovine hepatic abscesses. | a microbiological study was made of 100 strains of fusobacterium necrophorum isolated from hepatic abscesses in bovine and ovine herds. differences between the biological activity and ribotypes within the two f. necrophorum subspecies were studied. conventional methods identified 89 isolates as f. necrophorum subsp. necrophorum and 11 as f. necrophorum subsp. funduliforme. for ribotyping, 50 strains (35 f.n. subsp. necrophorum, 11 f.n. subsp. funduliforme and 4 reference strains) were digested w ... | 1998 | 16887645 |
| soil survival of escherichia coli o157:h7 acquired by a child from garden soil recently fertilized with cattle manure. | this investigation was conducted to determine the survival of a naturally occurring escherichia coli o157:h7 in garden soil linked to a sporadic case of e. coli o157 infection in minnesota. | 2006 | 16882151 |
| dissemination and persistence of shiga toxin-producing escherichia coli (stec) strains on french dairy farms. | some shiga toxin-producing escherichia coli strains (stec), and in particular e. coli o157:h7, are known to cause severe illness in humans. stec have been responsible for large foodborne outbreaks and some of these have been linked to dairy products. the aim of the present study was to determine the dissemination and persistence of stec on 13 dairy farms in france, which were selected out of 151 randomized dairy farms. a total of 1309 samples were collected, including 415 faecal samples from cat ... | 2006 | 16837144 |
| bovine serum amyloid a3 gene structure and promoter analysis: induced transcriptional expression by bacterial components and the hormone prolactin. | regulation of the bovine saa3 promoter in response to signaling molecules associated with lactation or bacterial infection was assessed using a luciferase reporter system. although the liver is the primary site for the production of acute phase proteins, typically serum amyloid a1 (saa1) and serum amyloid a2 (saa2), analysis of the differential expression of serum amyloid a3 (saa3) by mammary epithelial cells is limited. gram-negative bacterial lipopolysaccharide (lps) and the gram-positive bact ... | 2006 | 16837143 |
| mouse stefins a1 and a2 (stfa1 and stfa2) differentiate between papain-like endo- and exopeptidases. | stefin a (stfa) acts as a competitive inhibitor of intracellular papain-like cysteine proteases which play important roles in normal cellular functions such as general protein turnover, antigen processing and ovarian follicular growth and maturation. in the mouse there are at least three different variants of stfa (stfa1, stfa2 and stfa3). recent genetic studies identified structural polymorphisms in stfa1 and stfa2 as candidates for aod1b, a locus controlling susceptibility to day three thymect ... | 2006 | 16831429 |
| use of a nonmedicated dietary supplement correlates with increased prevalence of streptomycin-sulfa-tetracycline-resistant escherichia coli on a dairy farm. | we examined how a dietary supplement affects the prevalence of antibiotic-resistant escherichia coli on a dairy farm in washington state. between 2001 and 2004 the prevalence of fecal e. coli strains resistant to streptomycin, sulfadiazine, and tetracycline (ssut strains) declined from 59.2% to 26.1% in the calf population. in 2003 the dairy discontinued use of a dietary supplement, and we hypothesized that the decline in prevalence of ssut strains was related to this change in management. to te ... | 2006 | 16820447 |
| simulation of normal, carrier and affected controls for large-scale genotyping of cattle for factor xi deficiency. | an insertion mutation within exon 12 of the factor xi gene has been described in holstein cattle. this has opened the prospect for large-scale screening of cattle using the polymerase chain reaction (pcr) technique for the rapid identification of heterozygous animals. to facilitate such a screening process, the mutant and normal alleles of factor xi gene, represented by 244- and 320-bp pcr amplified fragments, were individually cloned in escherichia coli using a multicopy plasmid cloning vehicle ... | 2006 | 16819712 |
| simulating escherichia coli o157:h7 transmission to assess effectiveness of interventions in dutch dairy-beef slaughterhouses. | beef contamination with escherichia coli o157:h7 (vtec) is an important food-safety issue. to investigate the effectiveness of interventions against vtec in dutch beef industrial slaughterhouses that slaughter 500 dairy cattle per day, a monte carlo simulation model was built. we examined seven carcass-antimicrobial interventions, namely: hot-water wash, lactic-acid rinse, trim, steam-vacuum, steam-pasteurization, hide-wash with ethanol and gamma irradiation, and their combinations. the estimate ... | 2006 | 16814887 |
| isolation and characterization of recombinant drosophila copia aspartic proteinase. | the wild type copia gag precursor protein of drosophila melanogaster expressed in escherichia coli was shown to be processed autocatalytically to generate two daughter proteins with molecular masses of 33 and 23 kda on sds/page. the active-site motif of aspartic proteinases, asp-ser-gly, was present in the 23 kda protein corresponding to the c-terminal half of the precursor protein. the coding region of this daughter protein (152 residues) in the copia gag gene was expressed in e. coli to produc ... | 2006 | 16813567 |
| bioassay-guided purification and identification of antimicrobial components in chinese green tea extract. | the chinese green tea extract was found to strongly inhibit the growth of major food-borne pathogens, escherichia coli o157:h7, salmonella typhimurium dt104, listeria monocytogenes, staphylococcus aureus, and a diarrhoea food-poisoning pathogen bacillus cereus, by 44-100% with the highest activity found against s. aureus and lowest against e. coli o157:h7. a bioassay-guided fractionation technique was used for identifying the principal active component. a simple and efficient reversed-phase high ... | 2006 | 16797571 |
| shiga toxin-producing escherichia coli in ground beef and lamb cuts: results of a one-year study. | shiga toxin-producing escherichia coli (stec) have been associated with a broad spectrum of diarrhoeal syndromes. some of these cases have been attributed to foods of bovine origin or other foods cross-contaminated by beef products or cow manure. the purpose of this study was to determine the pattern of stec distribution in selected red meats over time. samples of ground beef and lamb cuts were collected over a 52-week period from 31 different outlets and 25 g portions were assayed for stec. ste ... | 2006 | 16793157 |
| adherent and invasive escherichia coli are associated with persistent bovine mastitis. | bovine mastitis caused by escherichia coli has traditionally been viewed as a transient infection. however, e. coli can also cause clonal persistent intramammary infection (imi) in dairy cows. in this study, we explored the possibility that e. coli strains associated with persistent imi are better able to adhere to, invade, survive and replicate in cultured mammary epithelial cells (mac-t) than transient strains, and examined their serotype, overall genotype, phylogenetic group, and the presence ... | 2006 | 16787715 |
| microbiological status of fresh beef cuts. | fresh beef samples (n = 1,022) obtained from two processing plants in the midwest (july to december 2003) were analyzed for levels of microbial populations (total aerobic plate count, total coliform count, and escherichia coli count) and for the presence or absence of e. coli o157:h7 and salmonella. a fresh beef cut sample was a 360-g composite of 6-g portions excised from the surface of 60 individual representative cuts in a production lot. samples of fresh beef cuts yielded levels of 4.0 to 6. ... | 2006 | 16786874 |
| [escherichia coli o157:h7 detection in fresh ground beef and hamburgers]. | escherichia coli o157:h7 is an emergent pathogen associated with food transmitted diseases. in 1982, escherichia coli o157:h7 was for the first time identified as the cause of two hemorrhagic colitis outbreaks in the united states. it is now well known that most cases of hemolytic uremic syndrome are caused by these bacteria. the objective of this work was to detect the microorganism in fresh ground beef and hamburgers. from april 2003 to august 2004 samples were taken at sale points of our supe ... | 2006 | 16784134 |
| [isolation of shiga toxin-producing escherichia coli strains from healthy cattle of the region of lower silesia]. | the aim of the study was to determine if cattle from the region of lower silesia is the reservoir of shiga toxin-producing e. coli strains (stec) and the analysis of virulence factors of isolated stec strains. the ability of tested animal strains to shiga toxin synthesis was analysed in cytotoxicity assay in vitro on vero cell line and then confirmed by detection of shiga toxin-encoding genes by pcr. stec strains were isolated from 12 (15,2%) of animals examined, 21,4% of these strains were obta ... | 2005 | 16773830 |
| ultra-fast tandem mass spectrometry scanning combined with monolithic column liquid chromatography increases throughput in proteomic analysis. | liquid chromatography combined with electrospray ionization mass spectrometry (lc/esi-ms) has been used successfully for the characterization of biomolecules in proteomics in the last few years. this methodology relied largely on the use of reversed-phase chromatography, in particular c18-based resins, which are suitable for separation of peptides. here we show that polymeric [polystyrene divinylbenzene] monolithic columns can be used to separate peptide mixtures faster and at a higher resolutio ... | 2006 | 16773668 |
| crystal structure of the second lns/lg domain from neurexin 1alpha: ca2+ binding and the effects of alternative splicing. | neurexins mediate protein interactions at the synapse, playing an essential role in synaptic function. extracellular domains of neurexins, and their fragments, bind a distinct profile of different proteins regulated by alternative splicing and ca2+. the crystal structure of n1alpha_lns#2 (the second lns/lg domain of bovine neurexin 1alpha) reveals large structural differences compared with n1alpha_lns#6 (or n1beta_lns), the only other lns/lg domain for which a structure has been determined. the ... | 2006 | 16772286 |
| development of bacteroides 16s rrna gene taqman-based real-time pcr assays for estimation of total, human, and bovine fecal pollution in water. | bacteroides species are promising indicators for differentiating livestock and human fecal contamination in water because of their high concentration in feces and potential host specificity. in this study, a real-time pcr assay was designed to target bacteroides species (allbac) present in human, cattle, and equine feces. direct pcr amplification (without dna extraction) using the allbac assay was tested on feces diluted in water. fecal concentrations and threshold cycle were linearly correlated ... | 2006 | 16751534 |
| molecular epidemiology of ceftiofur-resistant escherichia coli isolates from dairy calves. | healthy calves (n = 96, 1 to 9 weeks old) from a dairy herd in central pennsylvania were examined each month over a five-month period for fecal shedding of ceftiofur-resistant gram-negative bacteria. ceftiofur-resistant escherichia coli isolates (n = 122) were characterized by antimicrobial resistance (disk diffusion and mic), serotype, pulsed-field gel electrophoresis subtypes, beta-lactamase genes, and virulence genes. antibiotic disk diffusion assays showed that the isolates were resistant to ... | 2006 | 16751500 |
| field trial evaluating the influence of prophylactic and therapeutic antimicrobial administration on antimicrobial resistance of fecal escherichia coli in dairy calves. | the objective of this study was to describe the influence of in-feed and therapeutic antimicrobials on resistance in commensal fecal escherichia coli isolated from preweaned calves. four groups of 30, day-old calf-ranch calves were enrolled and raised until 4 weeks of age. groups 1 to 3 were raised without antimicrobials in the feed. group 1 was isolated from the other groups and received no antimicrobial therapy. group 2 was housed on the calf ranch and did not receive antimicrobial therapy, wh ... | 2006 | 16751491 |
| use of a simulation model to evaluate sampling strategies for characterization of antimicrobial resistance in non-type-specific escherichia coli isolated from dairy cows. | to evaluate various sampling strategies for potential use in measuring prevalence of antimicrobial susceptibility in cattle. | 2006 | 16740086 |
| demonstration of methods to reduce e. coli runoff from dairy manure application sites. | contamination by bacteria is a leading cause of impairment in u.s. waters, particularly in areas of livestock agriculture. we evaluated the effectiveness of several practices in reducing escherichia coli levels in runoff from fields receiving liquid dairy (bos taurus) manure. runoff trials were conducted on replicated hay and silage corn (zea mays l.) plots using simulated rainfall. levels of e. coli in runoff were approximately 10(4) to 10(6) organisms per 100 ml, representing a significant pol ... | 2006 | 16738394 |
| hybridase activity of human ribonuclease-1 revealed by a real-time fluorometric assay. | human ribonuclease-1 (hrnase-1) is an extracellular enzyme found in exocrine pancreas, blood, milk, saliva, urine and seminal plasma, which has been implicated in digestion of dietary rna and in antiviral host defense. the enzyme is characterized by a high catalytic activity toward both single-stranded and double-stranded rna. in this study, we explored the possibility that hrnase-1 may also be provided with a ribonuclease h activity, i.e. be able to digest the rna component of rna:dna hybrids. ... | 2006 | 16738129 |
| thionein can serve as a reducing agent for the methionine sulfoxide reductases. | it has been generally accepted, primarily from studies on methionine sulfoxide reductase (msr) a, that the biological reducing agent for the members of the msr family is reduced thioredoxin (trx), although high levels of dtt can be used as the reductant in vitro. preliminary experiments using both human recombinant msrb2 (hmsrb2) and msrb3 (hmsrb3) showed that although dtt can function in vitro as the reducing agent, trx works very poorly, prompting a more careful comparison of the ability of dt ... | 2006 | 16735467 |
| cloning and expression of recombinant mpb70 protein antigen from mycobacterium bovis bcg for diagnosis of tuberculosis. | in a search for developing new skin test reagents, mpb70 protein antigen was a candidate antigen for the diagnosis of bovine tuberculosis. first m. bovis bcg genomic dna was extracted purified and the mpb70 gene was amplified by pcr. the gene was then ligated to an expression vector, pqe. after transformation of the expression e. coil m15 host strain with the pqe plasmid, the expression was induced using 10 mm of iptg. two bands were seen in the sds-page analysis the 44 and 50 kda represents the ... | 2004 | 16734114 |
| spontaneous uterine infections are associated with elevated prostaglandin f(2)alpha metabolite concentrations in postpartum dairy cows. | postpartum holstein (n=21) and jersey (n=4) cows were used to determine if uterine infections are associated with elevated plasma concentrations of 13,14-dihydro-15-keto-prostaglandin f(2)alpha (pgfm). based upon clinical examinations and bacterial content of intrauterine fluid samples, cows detected with uterine infections between 21 and 28 d post partum were used (infected; n=14). these cows were matched with herdmates that were free of infection (control; n=11). beginning on the day the cows ... | 1994 | 16727399 |
| absorption of escherichia coli endotoxin (lipopolysaccharide) from the uteri of postpartum dairy cows. | an experiment was conducted to test the hypothesis that escherichia coli (e. coli ) endotoxin is readily absorbed from uteri of early postpartum cows and that the absorbed endotoxin provokes systemic relcase of prostaglandins. eleven postpartum holstein dairy cows (aged 3 to 7 yr) with normal puerperium were selected and divided into a treatment group (n=7), which received intrauterine infusions of e. coli endotoxin, and a control group (n=4), which received intrauterine infusions of 10 ml of sa ... | 1990 | 16726796 |
| the effect of escherichia coli endotoxin on luteal function in holstein heifers. | this study was undertaken to elucidate the possible role of endotcxin in mediating premature luteolysis in the well- documented phenomenon of short estrous cycles in postpartum dairy cows. four groups of holstein heifers (n = 4 to 6 each) received either intrauterine infusion of sterile culture medium (group i); intrauterine infusion of escherichia coli (e. coli ) endotoxin (5 mug/kg) in sterile culture medium (group ii); intrauterine administration of 10 ml of a 24-h culture of a strain of e. c ... | 1990 | 16726760 |
| incidence and treatments of postpartum reproductive problems in a dairy herd. | incidence of assisted births, retained fetal membranes (rfm), and metritis were recorded in one hundred dairy cows from parturition through 14 days post-calving. manual removal of rfm was not attempted. all rfm were excised inside the vulva and observations of natural rfm expulsion were recorded. fifteen of 100 cows (15%) had assisted births, 27 (27%) had rfm, 8 (8%) had primary metritis not associated with other postpartum reproductive problems, and 26 (26%) had secondary metritis. uterine swab ... | 1982 | 16725716 |
| the effect of the presence of globular proteins and elongated polymers on enzyme activity. | we have studied the effect of a crowded (macromolecular) solution on reaction rates of the decarboxylating enzymes urease, pyruvate decarboxylase and glutamate decarboxylase. a variety of crowding agents were used including haemoglobin, lysozyme, various dextrans and polyethylene glycol. enzyme reaction rates of all three enzymes show two different types of effect that separate the globular proteins from the polysaccharides/polymers. increasing concentration of globular proteins caused a dramati ... | 2006 | 16720113 |
| characterization of escherichia coli isolated from carcasses of beef cattle during their processing at an abattoir in brazil. | beef carcass sponge samples collected between march 2003 and august 2005 at an abattoir in brazil were surveyed for the presence of shiga toxin-producing escherichia coli (stec). only one carcass among the 80 tested showed a stec, stx2-encoding gene by pcr amplification. the frequency of carcass contamination by e. coli during processing was tested at three situations, respectively: preevisceration, postevisceration and postprocessing, during the rain and dry seasons. the prevalence of e. coli a ... | 2006 | 16720056 |
| inhibition of bacteriophage m13 replication with esterified milk proteins. | esterified milk proteins [methylated (met) or ethylated (et) alpha-lactalbumin (ala), beta-lactoglobulin (blg), and beta-casein (bcn)], unmodified native milk proteins, and native basic proteins (calf thymus histone and hen egg white lysozyme) were tested for their antiviral activity against the bacteriophage m13 and for their influence on its replication (except bcn). all esterified milk proteins showed an antiviral activity against the bacteriophage m13, proportional to the extent of esterific ... | 2006 | 16719499 |
| thermal inactivation studies of escherichia coli o157:h7, salmonella, and listeria monocytogenes in ready-to-eat chicken-fried beef patties. | thermal inactivation studies were used to determine the d- and z-values of escherichia coli o157:h7, salmonella, and listeria monocytogenes in ready-to-eat chicken-fried beef patties. inoculated meat was packaged in sterile bags, which were immersed in a circulated water bath and held at 55, 57.5, 60, 62.5, 65, 67.5, and 70 degrees c for different lengths of time. d- and z-values were determined with a linear regression model. average d-values at temperatures 55 to 70 degrees c were 27.62 to 0.0 ... | 2006 | 16715808 |
| attachment of shiga toxigenic escherichia coli to beef muscle and adipose tissue. | shiga toxigenic escherichia coli (stec) serotypes are important foodborne pathogens that cause gastrointestinal disease worldwide. an understanding of how stec strains attach to surfaces may provide insight into the potential persistence of and contamination with stec in food environments. the initial attachment of a selection of stec serotypes to beef muscle and adipose tissue was evaluated for isolates grown in planktonic and sessile culture. initial experiments were performed to determine whe ... | 2006 | 16715795 |
| the peripheral stalk of the mitochondrial atp synthase. | the peripheral stalk of f-atpases is an essential component of these enzymes. it extends from the membrane distal point of the f1 catalytic domain along the surface of the f1 domain with subunit a in the membrane domain. then, it reaches down some 45 a to the membrane surface, and traverses the membrane, where it is associated with the a-subunit. its role is to act as a stator to hold the catalytic alpha3beta3 subcomplex and the a-subunit static relative to the rotary element of the enzyme, whic ... | 2006 | 16697972 |
| analysis of fimbrial gene clusters and their expression in enterohaemorrhagic escherichia coli o157:h7. | the sequence of two enterohaemorrhagic escherichia coli (ehec) o157:h7 strains reveals the possession of at least 16 fimbrial gene clusters, many of the chaperone/usher class. the first part of this study examined the distribution of these clusters in a selection of ehec/epec (enteropathogenic e. coli) serotypes to determine if any were likely to be unique to e. coli o157:h7. six of the clusters, as determined by the presence of amplified main subunit or usher gene sequences, were detected only ... | 2006 | 16689724 |
| interleukin-8 expression by mammary gland endothelial and epithelial cells following experimental mastitis infection with e. coli. | epithelial and endothelial cells play a pivotal role in initiating and controlling the movement of leukocytes into tissues during inflammation through the production of cytokines and chemokines such as interleukin-8 (il-8). in situ hybridization with an il-8 riboprobe was used to determine il-8 mrna expression by mammary gland epithelial and endothelial cells in cows with experimental escherichia coli mastitis. epithelial cells of the gland, especially surrounding the alveoli, had increased il-8 ... | 2006 | 16675015 |
| isolation, characterization, and epidemiological assessment of shiga toxin-producing escherichia coli o84 isolates from new zealand. | shiga toxin-producing escherichia coli o84 isolates (n = 22) were examined using culture- and molecularly based methods in order to compare their phenotypic and genotypic characteristics. these analyses directly linked shiga toxin-producing escherichia coli o84 isolates from cattle and sheep with human isolates indicating that new zealand livestock may be a reservoir of infection. | 2006 | 16672424 |
| molecular cloning and functional expression of rabbit alpha2-antiplasmin. | the rabbit is frequently employed in small animal models of in-vivo coagulation and fibrinolysis, but the degree to which its plasma proteins resemble their human counterparts is incompletely known. our aims were: to determine the nucleotide sequence of a full-length rabbit liver alpha(2)-antiplasmin (alpha(2)ap) cdna; compare it with its human, murine, and bovine counterparts; and express it in functional form. partial cdnas encoding 5' and 3' portions of the alpha(2)ap mrna were obtained by re ... | 2006 | 16651871 |
| real-time reverse transcription-multiplex pcr for simultaneous and specific detection of rfbe and eae genes of escherichia coli o157:h7. | a real-time reverse transcription multiplex polymerase chain reaction (rrt-mpcr) was developed for detection of mrna encoded by rfbe and eae genes of enterohemorrhagic escherichia coli (ehec) o157:h7. a 129-bp and a 106-bp sequence specific to rfbe and eae, respectively, were targeted for reverse transcription, amplification, and real-time detection. a single-step rt-pcr kit containing a mixture of reverse transcriptases converted mrna into cdna, which was subsequently amplified by taq polymeras ... | 2006 | 16644181 |
| atr-ftir redox difference spectroscopy of yarrowia lipolytica and bovine complex i. | atr-ftir spectroscopy in combination with electrochemistry has been applied to the redox centers of yarrowia lipolytica complex i. the redox spectra show broad similarities with previously published data on escherichia coli complex i and with new data here on bovine complex i. the spectra are dominated by amide i/ii protein backbone changes. comparisons with redox ir spectra of small model ferredoxins demonstrate that these amide i/ii changes arise primarily from characteristic structural change ... | 2006 | 16634627 |
| the escherichia coli o157 flagellar regulatory gene flhc and not the flagellin gene flic impacts colonization of cattle. | a virulent european escherichia coli o157:h- isolate is nonmotile due to a 12-bp deletion in the flagellar regulatory gene flhc. to investigate the contribution of flhc in the relationship between e. coli o157:h7 and cattle, we constructed a similar flhc regulatory mutant in the well-characterized strain atcc 43894. there was no difference in the growth rate between the wild type and this regulatory mutant, but phenotypic arrays showed substrate utilization differences. survival in the bovine ga ... | 2006 | 16622228 |
| interferon-alpha genes from bos and bubalus bubalus. | interferon-a genes were cloned from six breeds of three species of two genera (three chinese native cattle breeds of yellow cattle, wild yak and huanhu domestic yak, one european breed of holstein cow, and two water buffalo breeds of fuan water buffalo and fuzhong water buffalo) by direct pcr. the pcr products were directly inserted into the expression vector to be sequenced and expressed. sequence analysis showed that ifn-a genes of six clones were composed of 498 nucleotides, encoding a mature ... | 2006 | 16621760 |
| development of a polymerase chain reaction assay for the detection of antibiotic resistance genes in community dna. | many methods are used to detect antibiotic resistance genes in samples. the objective of the study reported here was to compare polymerase chain reaction (pcr) analysis of community dna with fecal culturing for detecting antibiotic resistance genes in cattle samples. in the laboratory-based portion of this study, known concentrations of an escherichia coli strain with 3 antibiotic resistance genes (cmy-2, flo, and cat) were added to feces from dairy cattle. these genes were used to assess the ef ... | 2006 | 16617698 |
| toxicological impact studies based on escherichia coli bacteria in ultrafine zno nanoparticles colloidal medium. | we report here preliminary studies of biocidal effects and cellular internalization of zno nanoparticles on escherichia coli bacteria. zno nanoparticles were synthesized in di(ethylene glycol) (deg) medium by forced hydrolysis of ionic zn2+ salts. particle size and shape were controlled by addition of small molecules and macromolecules such as tri-n-octylphosphine oxide, sodium dodecyl sulfate, polyoxyethylene stearyl ether, and bovine serum albumin. transmission electron microscopy (tem) and x- ... | 2006 | 16608300 |
| evaluation of a pcr detection method for escherichia coli o157:h7/h- bovine faecal samples. | combinations of pcr primer sets were evaluated to establish a multiplex pcr method to specifically detect escherichia coli o157:h7 genes in bovine faecal samples. | 2006 | 16599993 |
| clonal dissemination of escherichia coli o157:h7 subtypes among dairy farms in northeast ohio. | to ascertain the extent to which indistinguishable strains of escherichia coli o157:h7 are shared between farms, molecular characterization was performed on e. coli o157:h7 isolates recovered during a longitudinal study of 20 dairy farms in northeast ohio. of the 20 dairy farms sampled, 16 were located in a primary area and 4 were located in two other distant geographical areas. a total of 92 e. coli o157:h7 isolates obtained from bovine fecal samples, water trough sediment samples, free-stall b ... | 2006 | 16597966 |
| purification and characterization of bovine steroid 21-hydroxylase (p450c21) efficiently expressed in escherichia coli. | steroid 21-hydroxylase, p450c21, is responsible for the conversion of progesterone and 17alpha-hydroxyprogesterone to their 21-hydroxylated derivatives. p450c21 has been poorly investigated because of difficulty in obtaining sufficient quantities of purified protein. to solve the problem, we have attempted to express the bovine p450c21 in escherichia coli as a stable form. the n-terminal membrane anchor and basic regions of p450c21 were replaced by the basic region of cyp2c3. the engineered p450 ... | 2006 | 16597434 |
| serotypes and analysis of distribution of shiga toxin producing escherichia coli from cattle and sheep in the lower north island, new zealand. | to serotype a subset of shiga toxin-producing escherichia coli (stec) isolates from cattle and sheep to determine whether any corresponding serotypes have been implicated in human diarrhoeal disease, both in new zealand and worldwide, and to examine the distribution of stec and enteropathogenic escherichia coli (epec) amongst cattle (calves, heifers and dairy) and sheep (lambs, rams and ewes), to assess whether carriage of identified bacterial genotypes may be associated with a particular age of ... | 2006 | 16596159 |
| characterization of escherichia coli isolates from different fecal sources by means of classification tree analysis of fatty acid methyl ester (fame) profiles. | microbial source tracking (mst) methods need to be rapid, inexpensive and accurate. unfortunately, many mst methods provide a wealth of information that is difficult to interpret by the regulators who use this information to make decisions. this paper describes the use of classification tree analysis to interpret the results of a mst method based on fatty acid methyl ester (fame) profiles of escherichia coli isolates, and to present results in a format readily interpretable by water quality mana ... | 2006 | 16570218 |
| adrenomedullin (am) and adrenomedullin binding protein (am-bp) in the bovine mammary gland and milk: effects of stage of lactation and experimental intramammary e. coli infection. | adrenomedullin (am) has been characterized as an endogenous tissue survival factor and modulator of many inflammatory processes. because of the increased susceptibility of the mammary gland to infection during the time surrounding parturition in the cow, we investigated how milk and tissue content of am and its binding protein (am-bp) might be affected by the stage of lactation and the udder health status. milk and mammary biopsy samples were obtained from holstein cows 21 days prior to and at v ... | 2007 | 16569490 |
| the influence of the nucleotide sequences of random shine-dalgarno and spacer region on bovine growth hormone gene expression. | to investigate the effects of the nucleotide sequences in shine-dalgarno (sd) and the spacer region (sd-atg) on bovine growth hormone (bgh) gene expression, the expression vectors under the control of the t7 promoter (pt7-7 vector) were constructed using bgh derivatives (bgh1 & bgh14) which have different 5'-coding regions and were induced in e. coli bl21(de3). oligonucleotides containing random sd sequences and a spacer region were chemically synthesized and the distance between the sd region a ... | 2006 | 16554719 |
| comparison of pcr-rflp and pfge for determining the clonality of enterohemorrhagic escherichia coli strains. | we report here on a comparative evaluation of pcr-restriction fragment length polymorphism (pcr-rflp) and pulsed-field gel electrophoresis (pfge) assays, and ascertain the clonal relationship between 13 enterohemorrhagic escherichia coli o157 : h7 strains isolated from fecal samples collected from three cows over a period of 2 months. pcr-rflp analysis was carried out with either bgli or ecorv digested la-pcr amplicons, generated by targeting region v of the stx-phage. while pcr-rflp analysis pl ... | 2006 | 16553842 |
| wildlife identified as major source of escherichia coli in agriculturally dominated watersheds by box a1r-derived genetic fingerprints. | the presence of escherichia coli in recreational and potable waters is a major concern to the general public as elevated levels of e. coli suggest the presence of pathogenic bacteria and viruses. unfortunately, traditional microbial techniques do not allow specific identification of the source of e. coli. this reduces the ability to target management practices that reduce bacterial contamination. in the finger lakes region of western new york, usa, wildlife resides in relatively high densities o ... | 2007 | 16551490 |
| microarray based comparison of two escherichia coli o157:h7 lineages. | previous research has identified the potential for the existence of two separate lineages of escherichia coli o157:h7. clinical isolates tended to cluster primarily within one of these two lineages. to determine if there are virulence related genes differentially expressed between the two lineages we chose to utilize microarray technology to perform an initial screening. | 2006 | 16539702 |
| coliform bacteria in sierra nevada wilderness lakes and streams: what is the impact of backpackers, pack animals, and cattle? | the presence of coliform bacteria indicates a watershed risk for harboring microbes capable of causing human disease. we hypothesized that water from watersheds that have different human- or animal-use patterns would have differing risks for the presence of coliform bacteria. methods; water was collected in wilderness areas of the sierra nevada range in california. a total of 60 sites from lakes or streams were selected to statistically differentiate the risk categories: 1) high use by backpacke ... | 2006 | 16538940 |
| results of the antimicrobial agent susceptibility study raised in a representative, cross-sectional monitoring study on a national basis. | the use of antimicrobial substances in human and veterinary medicine inevitably results in a selection pressure for drug resistance in exposed bacteria. preventive measures, apt to avoid the consequent development of new resistances and selection for existing ones, respectively, have to be elaborated. moreover, it has to be ensured that neither resistant bacteria nor resistance genes are spread to and consequently via the food chain. respiratory diseases as well as mastitis in dairy cattle belon ... | 2006 | 16531116 |
| [recombinant non-structural 3a, 3b and 3ab proteins of foot-and-mouth disease virus: use for differentiation of vaccinated and infected cattle]. | recombinant proteins 3a, 3b and 3ab were obtained by expression in escherichia coli and purified by metal-chelate chromatography. the proteins were used as antigens in indirect elisa to differentiate vaccinated and infected cattle. while testing 200 sera from cattle 3a-elisa was more sensitive and specific than 3b- and 3ab-elisa. compared with "chekit fmd-3abc", 3a-elisa showed the same level of specificity and higher level of sensitivity. | 2006 | 16523703 |
| a novel adsorbent of circulating bacterial toxins and cytokines: the effect of direct hemoperfusion with ctr column for the treatment of experimental endotoxemia. | the current study examined the ability of a new adsorbent, ctr, to remove enterotoxins, toxic shock syndrome toxin-1 (tsst-1), and cytokines from blood and/or serum in vitro and the effects of the extracorporeal treatment with ctr column on mortality rate and inflammatory responses to endotoxic shock in vivo. | 2006 | 16521274 |
| analysis of the clonal relationship of shiga toxin-producing escherichia coli serogroup o165:h25 isolated from cattle. | variations in time and space of a clonal group of escherichia coli o165:h25 on a cattle farm were monitored. the virulence marker pattern (stx genes, eae gene, hly(ehec) gene, katp gene, espp gene, efa gene) suggests that e. coli o165:h25 of bovine origin may represent a risk for human infection. | 2006 | 16517683 |
| a retrospective study of the aetiology and temporal distribution of bovine clinical mastitis in smallholder dairy herds in the dar es salaam region of tanzania. | a 31-year record-based retrospective study was carried out to determine the aetiology and temporal distribution of bovine clinical mastitis in smallholder dairy herds in the dar es salaam region of tanzania over the period november 1971-december 2002. laboratory information on 1964 quarter samples from 1365 cows in 281 smallholder dairy herds were retrieved, compiled and studied. eighty-eight percent of the quarter samples were culture-positive and the predominant mastitis pathogens isolated wer ... | 2007 | 16516507 |
| in vivo expression of the antimicrobial defensin and lactoferrin proteins allowed by the strategic insertion of introns adequately spliced. | a major limitation of conventional shuttle expression system, when cloning a bactericidal gene, is the basal expression level in bacteria, which is lethal. although the expression level is low, the bactericidal feature inherent to the molecule leads to subsequent failure to recover intact transformants when the related gene is cloned into a conventional expression vector. contrary to popular belief, the human cytomegalovirus immediate-early region 1 promoter (cmv), which is to date one of the mo ... | 2006 | 16516411 |
| cloning, expression, purification, crystallization and preliminary crystallographic study of the protein module (biv2-helix) in the fusion core of bovine immunodeficiency-like virus (biv) gp40. | the fusion core of bovine immunodeficiency virus (biv) gp40 is proposed to be involved in membrane fusion. however, no crystal structures are yet available. a predicted protein module biv2-helix of bivgp40 has been expressed in escherichia coli and purified by chromatography. recombinant biv2-helix was crystallized using the hanging-drop vapour-diffusion technique at 291 k. the crystals were grown in mpd and belonged to the primitive rhombohedral space group r3, with unit-cell parameters a = 39. ... | 2005 | 16511008 |
| phosphorus and sediment loss in a catchment with winter forage grazing of cropland by dairy cattle. | the loss of phosphorus and sediment to surface waters can impair their quality. it was hypothesized that the practice of winter grazing dairy cattle on cropland of moderate slope (5-20%) would exacerbate the loss of p and suspended sediment (ss) from land to water. in a small (4.3 ha) catchment two flumes were installed, upstream and downstream of one field (about 2 ha) that had been cropped for 2 yr and grazed in winter (june-july) by dairy cattle. flow proportional samples were taken and measu ... | 2006 | 16510702 |
| comparison of e. coli o157 and shiga toxin-encoding genes (stx) prevalence between ohio, usa and norwegian dairy cattle. | environmental and food contamination with shiga toxin-producing escherichia coli (stec) pose a threat to public health worldwide, with notable geographic differences in incidence of human disease caused by these organisms. the prevalence of e. coli o157 and total stx-positive specimens collected from mature dairy cattle in ohio and norwegian dairy farms was compared using identical laboratory methods in a cross-sectional survey. e. coli o157 was isolated from 5/750 (0.66%) of ohio dairy cows fro ... | 2006 | 16504323 |
| prevalence and characterization of escherichia coli o157 isolates from minnesota dairy farms and county fairs. | samples were collected from 26 organic and conventional farms and 12 county fairs in minnesota during 2001 and 2002 to identify the presence of escherichia coli o157. immunomagnetic separation was used for isolation of e. coli o157. isolates were further characterized by the presence of virulence marker genes (stx1, stx2, eaea, e-hly, katp, etpd, and espp), antimicrobial susceptibility profiles, and genotypes. during 2001, e. coli o157 was isolated from 16 (5.2%) of 305 fecal samples and from 7 ... | 2006 | 16496562 |
| biosynthetic 13c labeling of aromatic side chains in proteins for nmr relaxation measurements. | site-specific 13c labeling offers a desirable means of eliminating unwanted relaxation pathways and coherent magnetization transfer in nmr relaxation experiments. here we use [1-13c]-glucose as the sole carbon source in the growth media for protein overexpression in escherichia coli. the approach results in specific incorporation of 13c at isolated positions in the side chains of aromatic amino acids, which greatly simplifies the measurements and interpretation of 13c relaxation rates in these s ... | 2006 | 16492013 |
| biochemical characterization of human enteropeptidase light chain. | the synthetic gene encoding human enteropeptidase light chain (l-hep) was cloned into plasmid pet-32a downstream from the gene of fusion partner thioredoxin immediately after the dna sequence encoding the enteropeptidase recognition site. the fusion protein thioredoxin (trx)/l-hep was expressed in escherichia coli bl21(de3). autocatalytic cleavage of the fusion protein and activation of recombinant l-hep were achieved by solubilization of inclusion bodies and refolding of trx/l-hep fusion protei ... | 2006 | 16489914 |
| fluorescently labeled collagen binding proteins allow specific visualization of collagen in tissues and live cell culture. | visualization of the formation and orientation of collagen fibers in tissue engineering experiments is crucial for understanding the factors that determine the mechanical properties of tissues. in this study, collagen-specific fluorescent probes were developed using a new approach that takes advantage of the inherent specificity of collagen binding protein domains present in bacterial adhesion proteins (cna35) and integrins (gst-alpha1i). both collagen binding domains were obtained as fusion pro ... | 2006 | 16476406 |
| characterization of cytokine expression in milk somatic cells during intramammary infections with escherichia coli or staphylococcus aureus by real-time pcr. | the expression of inflammatory cytokines, including interleukin (il)-6, il-8, il-12, granulocyte macrophage-colony stimulating factor (gm-csf), tumor necrosis factor (tnf)-alpha, and interferon (ifn)-gamma, by milk somatic cells was characterized by real-time polymerase chain reaction (pcr) in dairy cows experimentally challenged with either e. coli (n = 8) or s. aureus (n = 8). the mrna abundance of a target gene was calibrated with that of a reference gene (beta-actin) and expressed as fold of ... | 2006 | 16472521 |
| cesarean section and ovariohysterectomy after severe uterine torsion in four cows. | to report a technique for intra-partum ovariohysterectomy (ovh) in cows after severe uterine compromise after uterine torsion. | 2006 | 16472302 |
| assessing the efficacy of within-animal control strategies against e. coli o157: a simulation study. | a stochastic simulation model was used to assess the efficacy of potential measures to control the levels of escherichia coli o157 within the bovine host. the model described e. coli o157 population sizes at several sites along the bovine gut and therefore only interventions that operate at an individual animal level could be evaluated. in order to use the model to evaluate the control strategies, it was necessary to make assumptions about how each strategy affected e. coli o157 populations in v ... | 2006 | 16464511 |
| bedding and within-pen location effects on feedlot pen runoff quality using a rainfall simulator. | soluble salts, nutrients, and pathogenic bacteria in feedlot-pen runoff have the potential to cause pollution of the environment. a 2-yr study (1998-1999) was conducted at a beef cattle (bos taurus) feedlot in southern alberta, canada, to determine the effect of bedding material [barley (hordeum vulgare l.) straw versus wood chips] and within-pen location on the chemical and bacterial properties of pen-floor runoff. runoff was generated with a portable rainfall simulator and analyzed for chemica ... | 2006 | 16455851 |
| role of coresistance in the development of resistance to chloramphenicol in escherichia coli isolated from sick cattle and pigs. | to determine the cause of persistent resistance to chloramphenicol (cp) after the ban on its use in food-producing animals in several countries. | 2006 | 16454626 |
| selection of an escherichia coli o157:h7 bacteriophage for persistence in the circulatory system of mice infected experimentally. | a bacteriophage lytic for escherichia coli o157:h7 was isolated from bovine manure. following in-vivo selection, the phage acquired the capacity to persist in the circulatory system of mice for at least 38 days. when mice were infected experimentally with e. coli o157:h7 (10(7) cfu/mouse), simultaneous injection of the mice with phage (10(8) pfu/mouse) cleared e. coli o157:h7 from the mice within 48 h. | 2006 | 16451412 |
| comparison of effects of high-pressure processing and heat treatment on immunoactivity of bovine milk immunoglobulin g in enriched soymilk under equivalent microbial inactivation levels. | immunoglobulin-rich foods may provide health benefits to consumers. to extend the refrigerated shelf life of functional foods enriched with bovine immunoglobulin g (igg), nonthermal alternatives such as high-pressure processing (hpp) may offer advantages to thermal processing for microbial reduction. to evaluate the effects of hpp on the immunoactivity of bovine igg, a soymilk product enriched with milk protein concentrates, derived from dairy cows that were hyperimmunized with 26 human pathogen ... | 2006 | 16448177 |
| escherichia coli with anti-o157:h7 activity isolated from bovine colon. | to isolate bacteria from bovine gastrointestinal tract and investigate their inhibitory effect on escherichia coli o157:h7 in vitro. | 2006 | 16430515 |
| acute experimental mastitis is not causal toward the development of energy-related metabolic disorders in early postpartum dairy cows. | twenty holstein cows in early lactation (7 d in milk) were administered 100 microg of escherichia coli lipopolysaccharide (lps) dissolved in 10 ml of sterile 0.9% nacl saline (treatment; trt) or 10 ml of sterile saline (control) into both right mammary quarters to test the hypothesis that acute experimental mastitis would have negative impacts on aspects of energy metabolism that might lead to the development of metabolic disorders. a primed continuous intravenous infusion (14-micromol/kg of bw ... | 2006 | 16428629 |
| salvage of focal cerebral ischemic damage by transfusion of high o2-affinity recombinant hemoglobin polymers in mouse. | cell-free hemoglobin solutions with high oxygen affinity might be beneficial for selectively delivering oxygen to ischemic tissue. a recombinant hybrid hemoglobin molecule was designed using the human alpha-subunit and the bovine beta-subunit, with placement of surface cysteines to permit disulfide bond polymerization of the tetramers. the resulting protein generated from an escherichia coli expression system had a molecular mass >1 mda, a p50 of approximately 3 torr, and a cooperativity of n = ... | 2006 | 16424069 |
| differentiation of faecal escherichia coli from humans and animals by multiple antibiotic resistance analysis. | multiple antibiotic resistance (mar) was performed on 128 escherichia coli isolates, recovered from faecal samples of humans and animals (cattle, goat, sheep) to determine and compare their antibiotic resistance patterns and to evaluate them statistically in order to specify the source of the faecal material. | 2006 | 16411923 |
| super-shedding cattle and the transmission dynamics of escherichia coli o157. | the prevalence of escherichia coli o157 displays striking variability across the scottish cattle population. on 78% of farms, in a cross-sectional survey of 952, no shedding of e. coli o157 was detected, but on a small proportion, approximately 2%, very high prevalences of infection were found (with 90-100% of pats sampled being positive). we ask whether this variation arises from the inherent stochasticity in transmission dynamics or whether it is a signature of underlying heterogeneities in th ... | 2006 | 16409660 |
| geographical variation in antibiotic resistance profiles of escherichia coli isolated from swine, poultry, beef and dairy cattle farm water retention ponds in florida. | the aim of this study was to assess geographical variation in multiple antibiotic resistance (mar) profiles of livestock escherichia coli as well as to evaluate the ability of mar profiles to differentiate sources of faecal pollution. | 2006 | 16405684 |
| quantitative analysis and evaluation of the solubility of hydrophobic proteins recovered from brain, heart and urine using uv-visible spectrophotometry. | there is a need for a simple method that can directly quantify hydrophobic proteins. uv-visible spectrophotometry was applied in the present study for this purpose. absorbance at lambda = 280 nm (a280) was detected for both escherichia coli membrane proteins and bovine serum albumin, whereas absorbance at lambda = 620 nm (a620) was only detected for e. coli membrane proteins. the a620 values of the brain samples were greater than those of heart samples when equal concentrations were used, regard ... | 2006 | 16402178 |
| prevalence and virulence factors of escherichia coli serogroups o26, o103, o111, and o145 shed by cattle in scotland. | a national survey was conducted to determine the prevalence of escherichia coli o26, o103, o111, and o145 in feces of scottish cattle. in total, 6,086 fecal pats from 338 farms were tested. the weighted mean percentages of farms on which shedding was detected were 23% for e. coli o26, 22% for e. coli o103, and 10% for e. coli o145. the weighted mean prevalences in fecal pats were 4.6% for e. coli o26, 2.7% for e. coli o103, and 0.7% for e. coli o145. no e. coli o111 was detected. farms with catt ... | 2006 | 16391103 |
| modeling and predicting the simultaneous growth of escherichia coli o157:h7 and ground beef background microflora for various enrichment protocols. | the simultaneous growth of escherichia coli o157:h7 (o157) and the ground beef background microflora (bm) was described in order to characterize the effects of enrichment factors on the growth of these organisms. the different enrichment factors studied were basal medium (trypticase soy broth and e. coli broth), the presence of novobiocin in the broth, and the incubation temperature (37 degrees c or 40 degrees c). bm and o157 kinetics were simultaneously fitted by using a competitive growth mode ... | 2006 | 16391052 |
| [identification on the immunogenic activity of recombinant rltb/ctb-lipl41/ 1 to leptospira interrogans and detection of lipl41 gene with its production]. | to construct prokaryotic expression systems of ltb/ctb-lipl41/1 fusion genes, identify immunogenic and adjuvant activities of the products as well as to understand the frequencies of lipl41 gene that carrying and expressing in l. interrogans wild strains and specific antibody levels in sera from patients with leptospirosis. | 2005 | 16390012 |
| association between indicators of cattle density and incidence of paediatric haemolytic-uraemic syndrome (hus) in children under 15 years of age in france between 1996 and 2001: an ecological study. | over the past years shiga-like toxin-producing escherichia coli (stec) o157:h7 emerged as an important cause of severe gastrointestinal illnesses and haemolytic-uraemic syndrome (hus) with up to 10% of children infected with stec developing hus. we conducted a geographical ecological study using the district as the statistical unit. for each district, we estimated the incidence of hus among children <15 years for the period 1996-2001 from national hus surveillance data and data obtained on cattl ... | 2006 | 16371171 |
| antimicrobial resistance and class 1 integrons in pathogenic escherichia coli from dairy farms. | the goal of this study was to assess the prevalence of antimicrobial resistance and class 1 integrons, including integron-associated genes, in 24 escherichia coli isolates from dairy farms. escherichia coli isolates (n = 14) from dairy cows with mastitis (ecdm), shiga toxin-producing (stec) o157:h7 from cull dairy cow fecal samples (n = 9) and bulk tank milk (n = 1) were evaluated for sensitivity to 19 antimicrobial agents used commonly in human and/or veterinary medicine. multiplex pcr was used ... | 2005 | 16366857 |