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genetic manipulation of campylobacter: evaluation of natural transformation and electro-transformation.two methods, natural transformation and electro-transformation, for the introduction of dna into nine strains of campylobacter jejuni were compared. both methods were successful with a limited number of strains. natural transformation was efficient only for the introduction of c. jejuni chromosomal dna, while electro-transformation was also applicable for the introduction of escherichia coli-derived vector dna into at least one c. jejuni strain. the efficiency of dna recombination after entry wa ...19938406035
studies on the pathophysiological mechanism of campylobacter jejuni-induced fluid secretion in rat ileum.calcium has been reported to play an important role in regulating the intestinal electrolyte transport via ca2+/calmodulin (cam) and/or protein kinase c (pkc) systems. the role of ca2+, cam and pkc in the pathogenesis of campylobacter jejuni-induced fluid accumulation was studied in vivo in ligated rat ileal loops. calcium ionophore a23187 (5 microm) and pkc activator, phorbol-12-myristate-13-acetate (pma, 100 micrograms kg-1) when injected alone induced fluid accumulation in the control loops. ...19938405940
isolation of campylobacter jejuni strains resistant to nalidixic acid and fluoroquinolones from children with diarrhea in athens, greece. 19938404924
chemical structures of the core regions of campylobacter jejuni serotypes o:1, o:4, o:23, and o:36 lipopolysaccharides. 19938404908
cloning and nucleotide sequence of the campylobacter jejuni gyra gene and characterization of quinolone resistance mutations.the gyra gene of campylobacter jejuni ua580, which encodes the a subunit of dna gyrase, was cloned and its nucleotide sequence was determined. an open reading frame of 2,589 nucleotides was identified, which could code for a polypeptide of 863 amino acids with a m(r) of 97 kda. both the nucleotide sequence and the putative amino acid sequence show ca. 50% identity with those of other gyra genes from gram-positive and gram-negative bacteria. the locations of the gyra gene on genome maps of both c ...19938384814
rotavirus-inhibitory activity in serial milk samples from mexican women and rotavirus infections in their children during their first year of life.a total of 75 children born in rural mexico were followed for diarrheal diseases and rotavirus (rv) excretion during the first year of life. for 18 children, an average of 14 serial breast milk samples were obtained between days 2 and 360 after delivery and were tested for rv-inhibitory activity. of these samples, 70, 62, and 85% showed inhibitory activity against serotype (st) 1 human rv, st4 human rv, and st3 simian rv, respectively; the median titers were 10, 10, and 20, respectively. some 89 ...19938384626
helicobacter acinonyx sp. nov., isolated from cheetahs with gastritis.four strains of a novel helicobacter species were isolated from the stomachs of cheetahs (acinonyx jubilatus) with gastritis. these isolates were phenotypically similar to helicobacter pylori. the isolates were gram-negative, spiral bacteria which grew under microaerophilic conditions at 37 degrees c, but not at 25 or 42 degrees c, and produced urease, catalase, oxidase, alkaline phosphatase, and gamma-glutamyl transpeptidase. the isolates did not ferment glucose, mannitol, inositol, sorbitol, r ...19938379970
tales of campylobacter jejuni. 19938379884
kinetic and antigenic characterization of altered protein synthesis by campylobacter jejuni during cultivation with human epithelial cells.cultivation of campylobacter jejuni with int 407 cell monolayer cultures results in new or enhanced synthesis of a number of proteins compared with bacteria cultured in the absence of the epithelial cells. these proteins were detected within 60 min after the addition of the bacteria to the epithelial cell cultures, and their synthesis was temporally associated with an increase in c. jejuni internalization. a rabbit antiserum raised against bacteria that were cultivated with int 407 cells recogni ...19938376841
application of polymerase chain reaction for the correlation of salmonella serovars recovered from greyhound feces with their diet.the polymerase chain reaction was employed to correlate salmonella serovars isolated from fecal material of greyhounds suffering from gastroenteritis with those isolated from the diet fed to the greyhounds prior to onset of diarrhea. kennels around the abilene, kansas, area were contacted and supplied with materials needed to collect a portion of the diet each day. with the onset of diarrhea, the kennels were instructed to ship the fecal material and diet from the previous 10 days to the laborat ...19938373851
pulsed-field gel electrophoretic analysis of campylobacter jejuni dna for use in epidemiological studies.the cleavage patterns of the genomic dna of 42 clinical isolates of campylobacter jejuni determined by pulsed-field gel electrophoresis (pfge) were compared with their lior and tck serotypes. the fragment patterns of dna obtained with sali and smai restriction enzymes did not always accord with the corresponding serotypes but strains of the same serotype could be further divided into subtypes by their cleavage patterns. this pfge method may prove useful for subclassifying c. jejuni.19938370943
a cytotoxicity test for the detection of campylobacter jejuni toxin.a 7-h 51cr-release cytotoxicity assay has been developed to detect the presence of campylobacter jejuni toxin in the culture supernatant. some of the culture supernatants have cytotoxic effects against labeled chicken lymphocytes, while some others do not have this effect. there is no direct correlation between the pathogenicity of the organisms and the presence of the toxin. the supernatant of c. jejuni retains its cytotoxicity after heating at 100 degrees c for 10 min.; therefore, it is a heat ...19938362493
colonization of chicks by motility mutants of campylobacter jejuni demonstrates the importance of flagellin a expression.campylobacter jejuni strain 81116 contains two flagellin genes, flaa and flab. wild-type (wt) bacteria express flaa only, but flab can be expressed under certain conditions. we have determined the importance of flagella for colonization of the avian caecum, which appears to be the natural environment for these bacteria. mutants in which flaa or flab, or both had been inactivated, and motility variants, were investigated. flagella are not a requisite for colonization, but mutants lacking both fla ...19938360610
peb1, the major cell-binding factor of campylobacter jejuni, is a homolog of the binding component in gram-negative nutrient transport systems.the protein peb1 (28 kda) is a common antigen and a major cell adherence molecule of campylobacter jejuni and campylobacter coli. we created a bank of chromosomal dna fragments of c. jejuni strain 81-176 using lambda gt11. screening this bank in escherichia coli y1090 cells with antibody raised against purified peb1 enabled us to isolate and to purify a clone with a 2.6-kilobase insert expressing an immunoreactive protein of 28 kda. dna sequencing revealed that the insert contains three complete ...19938360165
direct polymerase chain reaction detection of campylobacter jejuni and campylobacter coli in raw milk and dairy products.a polymerase chain reaction (pcr) method designed to sensitively detect and identify campylobacter jejuni and campylobacter coli without the need for isolating and culturing strains is described. the intergenic sequence between the flagellin genes flaa and flab was amplified and characterized with a triple primer or seminested primer approach. a total of 50 bacterial strains, 27 of c. jejuni and c. coli and 23 of other species, were tested, giving no false-positive or false-negative results. the ...19938357250
[campylobacter jejuni sepsis and chronic terminal kidney insufficiency]. 19938355552
cholecystitis caused by campylobacter jejuni. 19938353233
role of infection due to campylobacter jejuni in the initiation of guillain-barré syndrome.recent reports suggest that infection with campylobacter jejuni, a common enteric pathogen, may cause guillain-barré syndrome (gbs) by triggering demyelination of peripheral nerves. gbs is preceded by an acute infectious illness (due to a variety of agents) in 50%-75% of cases; the onset of neurological symptoms is preceded by diarrhea in 10%-30% of cases. in the last decade, more than 20 published anecdotal reports and case series have described patients with c. jejuni infection documented 1-3 ...19938353228
common diarrhea pathogens and the risk of dehydration in young children with acute watery diarrhea: a case-control study.the role of common diarrheal pathogens in dehydration was examined in children with acute watery diarrhea who attended the treatment center of the international centre for diarrhoeal disease research, bangladesh, in dhaka. two hundred sixty-nine children with moderate or severe dehydration were matched with 700 children with no dehydration. vibrio cholerae o1 infections were 5.5 times more likely to be associated with dehydration than in cases without this agent. no significant association could ...19938352397
enterotoxigenic bacteria in the sudden infant death syndrome.faecal samples from 123 infants who died with sudden infant death syndrome (sids) and from a comparative group of 52 age-matched babies were analysed for toxigenic bacteria and their toxins. serum samples from the sids infants were also analysed for these toxins. a significantly higher proportion of toxigenic bacteria and their toxins were found in faecal samples of sids babies than in samples from the comparative group. these toxins were also found in serum from the sids babies. clostridium per ...19938345506
unusual microtubule-dependent endocytosis mechanisms triggered by campylobacter jejuni and citrobacter freundii.bacterial invasion of six different human epithelial cell lines showed that some strains of the intestinal pathogen campylobacter jejuni invaded intestinal cell lines at a level 10(2)-10(4) times higher than reported previously for other campylobacter strains. separately, urinary tract isolates of citrobacter freundii triggered a high-efficiency invasion of bladder cells. use of multiple inhibitors with known effects on eukaryotic cell structures/processes allowed us to define in these genetical ...19938341714
cloning and characterization of the gamma-glutamyl phosphate reductase gene of campylobacter jejuni.the gamma-glutamyl phosphate reductase gene, proa, of campylobacter jejuni was isolated from a recombinant pbr322 clone. a hindiii fragment of the insert containing the gene was subcloned into puc19 and sequenced in both orientations. the deduced amino acid sequence of gamma-glutamyl phosphate reductase (ec 1.2.1.41) of c. jejuni exhibits 36.4% identity to that of escherichia coli and 36.0% identity to serratia marcescens. two highly conserved regions in the amino acid sequence were identified f ...19938341262
the spectrum of immune responses to campylobacter jejuni and glycoconjugates in guillain-barré syndrome and in other neuroimmunological disorders.an acute infectious illness frequently precedes the guillain-barré syndrome. recently, campylobacter jejuni was claimed to be a predominant precipitating agent that may also trigger a humoral immune response to glycoconjugates of peripheral myelin in guillain-barré syndrome. because of conflicting reports, we determined the frequency of a recent infection with c. jejuni in 38 patients with guillain-barré syndrome using a highly sensitive and specific immunoblot technique, and of the presence of ...19938338338
serum antibodies to gm1, gd1b, peripheral nerve myelin, and campylobacter jejuni in patients with guillain-barré syndrome and controls: correlation and prognosis.serum antibodies to monosialoganglioside (gm1), disialoganglioside (gd1b), and campylobacter jejuni, measured by enzyme-linked immunosorbent assay and serum antibodies to peripheral nerve myelin, measured by the c1 fixation and transfer assay, were studied in 58 acute-phase patients with guillain-barré syndrome (gbs), 42 disease controls, and 29 normal controls. anti-peripheral nerve myelin antibodies were elevated in 57 of 58 patients with gbs compared with controls, whereas only 8.6% had incre ...19938338337
newborn piglet model for campylobacteriosis.an in vivo model system for human campylobacteriosis has been developed in which colostrum-deprived newborn piglets are orally challenged with an invasive strain of campylobacter jejuni. piglets developed clinical symptoms and histopathological lesions similar to those observed in humans infected with c. jejuni. gross lesion examination at autopsy revealed the presence of edema, hyperemia, and mucus. histopathologic examinations by light and transmission electron microscopy demonstrated damage t ...19938335377
isolation and characterization of two campylobacter glycine-extracted proteins that bind to hela cell membranes.two immunogenic proteins of 27 (cbf1) and 29 (cbf2) kda from enteropathogenic campylobacter species appear to bind to mammalian cells. we purified these two proteins from a pathogenic and adherent campylobacter jejuni strain to homogeneity by using acid extraction, preparative gel electrophoresis, and electroelution. polyclonal rabbit antisera to these proteins were prepared. immunologic studies indicate that cbf1 corresponds to the peb1 and cbf2 corresponds to the peb4 described by pei et al. ( ...19938335374
plasma level of antisecretory factor (asf) and its relation to post-weaning diarrhoea in piglets.antisecretory factor (asf) is a regulatory peptide which counteracts diarrhoea in the pig; asf is rapidly absorbed from the pig intestine, and significantly reduces the incidence of neonatal diarrhoea in the suckling offspring. asf is synthesized in the central nervous system, and released to the blood stream via the pituitary gland. in two different experiments (n = 8 and n = 4), the blood concentration of asf was followed in 5-weeks old piglets from day 7 before weaning up to day 12 days after ...19938322543
development of species-specific dna probes for campylobacter jejuni, campylobacter coli, and campylobacter lari by polymerase chain reaction fingerprinting.the application of polymerase chain reaction (pcr) fingerprinting assays enables discrimination between species and strains of microorganisms. pcr primers aiming at arbitrary sequences in combination with primers directed against the repetitive extragenic palindrome (rep) or enterobacterial repetitive intergenic consensus (eric) motifs generate isolate-specific dna banding patterns. analysis of these pcr fingerprints obtained for 33 isolates of campylobacter jejuni, 30 isolates of campylobacter ...19938314996
diarrhea in u.s. troops deployed to thailand.ninety-five (28%) of 333 u.s. military personnel deployed to ubonratchathani, thailand, for 1 month in february 1993 developed diarrhea. campylobacter jejuni was identified in 6 (25%), attaching and effacing escherichia coli was identified in 3 (13%), nontyphoidal salmonella spp. were identified in 2 (8%), and rotavirus was identified in 1 (4%) of 24 persons who had diarrhea and submitted specimens.19938308137
use of an immunoglobulin m containing preparation for treatment of two hypogammaglobulinemic patients with persistent campylobacter jejuni infection.this report describes two hypogammaglobulinemic patients with persistent campylobacter jejuni infections in spite of igg substitution and antibiotic therapy. since serum bactericidal activity (sba) depends on igm, these patients were each treated with six doses of an igm-containing immunoglobulin preparation (pentaglobin) at three-week intervals. during igg therapy sba was not seen in either patient. however, one hour following administration of the igm preparation, sba increased to 90%. just be ...19938307048
the microbiology of acute diarrhoeal disease in the eastern province of saudi arabia.a prospective study of acute diarrhoeal diseases in the eastern province of saudi arabia was carried out over a 19-month period to determine aetiology, risk factors and other epidemiological characteristics. of the 853 subjects studied, 344 were cases and 509 controls. more cases were seen in children than in adults. enteric pathogens were detected in 49% of the cases, but none in the controls. of the pathogens, 68% were bacterial with salmonella (34%) and shigella species (14.7%) being the most ...19938306900
intestinal b cell defects in common variable immunodeficiency.the humoral immune system of the small intestine of 17 patients with common variable immunodeficiency (cvid) was studied by immunohistology using antibodies specific for iga1,2, igm, igg1-4, the j chain and the secretory component (sc). iga1,2+, igg2+ and igm+ lamina propria b cells were totally lacking in 65% (11/17), 41% (7/17) and 18% (3/17) of cvid patients, respectively. one patient exhibited an isolated iga1 subclass deficiency. the proportion of plasma cells in conventionally stained hist ...19948306494
development and characterization of reca mutants of campylobacter jejuni for inclusion in attenuated vaccines.isogenic reca mutants of campylobacter jejuni have been constructed for evaluation of their usefulness in attenuated vaccines against this major worldwide cause of diarrhea. the reca+ gene of c. jejuni 81-176 was cloned by using degenerate primers to conserved regions of other reca proteins in a pcr. the c. jejuni reca+ gene encodes a predicted protein with an m(r) of 37,012 with high sequence similarity to other reca proteins. the termination codon of the reca+ gene overlaps with the initiation ...19948300203
[the hemolytic-uremic syndrome in enterocolitis caused by campylobacter jejuni].in the submitted case-history the authors describe the clinical course of haemolytic-uraemic syndrome (hus) during campylobacter infection in a two-year-old boy. on the described case the authors wish to confirm that in the manifestation of hus in childhood not only infections caused by the usual microbial agents can participate but also campylobacter jejuni.19938299194
evidence for the presence of a receptor for the cytolethal distending toxin (cldt) of campylobacter jejuni on cho and hela cell membranes and development of a receptor-based enzyme-linked immunosorbent assay for detection of cldt.using ligand blotting, it was found that partially purified cytolethal distending toxin prepared from an enterotoxigenic strain of campylobacter jejuni, bound to two peptides of molecular masses of approximately 59 kda and 45 kda and to a single peptide of 59 kda in protein blots prepared from hela and cho cell membranes, respectively. in contrast, labile toxin of escherichia coli and cholera toxin bound to a single peptide of the same molecular mass (15 kda) on protein blots prepared from both ...19938288105
lipopolysaccharides of campylobacter jejuni serotype o:19: structures of core oligosaccharide regions from the serostrain and two bacterial isolates from patients with the guillain-barré syndrome.lipopolysaccharides from phenol-water extraction of cells of campylobacter jejuni serotype o:19 were separated into a water-soluble gel of low m(r) and a water-soluble component of high m(r). acetic acid hydrolysis of the ketosidic linkages to lipid a furnished respectively a core oligosaccharide, the structure of which is reported herein, and an o antigenic polysaccharide. structural investigations were performed on the o-deacetylated lipopolysaccharide of low m(r), the liberated core oligosacc ...19948286348
efficacy of filter types for detecting campylobacter jejuni and campylobacter coli in environmental water samples by polymerase chain reaction.a previously developed polymerase chain reaction (pcr) amplification of a target region in the flaa campylobacter flagellin gene was evaluated and adapted for use with environmental water samples. the ability to detect campylobacter jejuni or campylobacter coli in seeded water samples was tested with various filters after concentration and freeze-thaw lysis of the bacterial cells. a nonradioactive probe for the amplified flagellin gene fragment detected as little as 1 to 10 fg of genomic dna and ...19938285708
[mucinase activity of helicobacter pylori: application of simplified mucinase test].we conducted the simplified mucinase tests about helicobacters (helicobacter pylori, helicobacter felis and helicobacter mustelae) and campylobacter jejuni, positive controls were pseudomonas maltophilia and pseudomonas cepacia. h. pylori, h. felis and c. jejuni showed low resolution activities for hog gastric mucin, however h. mustelae had no detectable the activities. this enzymatic activity varies considerably by cultural condition of h. pylori, therefore it is difficult to compare the activi ...19938283626
[five cases of campylobacter jejuni enteritis accompanied with guillain-barré syndrome].17 cases of guillain-barré syndrome (gbs) were admitted to the first hospital of beijing medical university and baoding hospital from july, 1991 to october, 1992. five patients had diarrhea before the onset of gbs and showed positive reaction for serum igm antibodies to campylobacter jejuni. they were considered to have campylobacter jejuni enteritis as well. this incidence of campylobacter jejuni enteritis in gbs patients was higher than in those with other neurological diseases and in normal c ...19938269767
short-term treatment of traveler's diarrhea with norfloxacin: a double-blind, placebo-controlled study during two seasons.clinical efficacy of norfloxacin for treatment of traveler's diarrhea in 106 finnish tourists vacationing in morocco was evaluated during two different seasons. when the criteria for diagnosis of traveler's diarrhea were fulfilled, norfloxacin (400 mg) or a placebo was given orally, twice daily for 3 days. all symptoms and signs subsided sooner in the norfloxacin group. the clearest difference was observed in the duration of diarrhea: 1.2 days in the norfloxacin group vs. 3.3 days in the placebo ...19938268363
campylobacter jejuni enterocolitis causing peritonitis, ileitis and intestinal obstruction.patients with campylobacter enterocolitis may come to laparotomy due to the severity of abdominal symptoms and signs, although only two patients with intestinal inflammation have been described and in neither was the histopathology documented. a case of a 52-year-old male who had a typical diarrhoeal illness of campylobacter enterocolitis diagnosed on stool culture is reported. despite appropriate treatment he developed signs and symptoms consistent with small intestinal obstruction. laparotomy ...19948267541
a community study on the aetiology of childhood diarrhoea with special reference to campylobacter jejuni in a semiurban slum of varanasi, india.in a community study of 607 diarrhoeal and 529 non-diarrhoeal (control) patients less than 5 years old carried out between august 1988 and july 1989, the campylobacter jejuni isolation rate was 4% in the diarrhoeal and 0.9% in the control group. it was the second most common bacterial enteropathogen isolated after escherichia coli. its incidence was more common among 1-2 year old children (4.8%) and during rainy season (july-october). features of dysentery were absent in c. jejuni diarrhoea. fin ...19938263306
campylobacter jejuni and guillain-barré syndrome.campylobacter jejuni is the most frequently identified bacterial cause of gastroenteritis in the uk, and commonly precedes guillain-barré syndrome (gbs). there is some evidence that the resultant neurological disease may be a more severe variant of gbs with predominant axonal degeneration. crossreactivity between neural antigens and c. jejuni may be one of the mechanisms by which gbs is triggered. unravelling the immunopathogenesis of gbs following c. jejuni enteritis may shed light on the way i ...19938255960
[erythema nodosum in pediatric patients. a study of 22 cases].in this study we report the cases of 22 pediatric patients with nodal erythema. the predominance of this condition in male patients was clear. etiological factors were determined in 77% of the patients. in our series, the principal cause was tuberculosis (36%). we would like to point out the etiological diversity in this small series of children: streptococcal infection, gastrointestinal infection with salmonella enteritidis or campylobacter jejuni, cat scratch disease, infectious mononucleosis, ...19938250429
survival of pathogenic bacteria during mesophilic anaerobic digestion of animal waste.the survival of pathogenic bacteria was investigated during the operation of a full-scale anaerobic digester which was fed daily and operated at 28 degrees c. the digester had a mean hydraulic retention time of 24 d. the viable numbers of escherichia coli, salmonella typhimurium, yersinia enterocolitica, listeria monocytogenes and campylobacter jejuni were reduced during mesophilic anaerobic digestion. escherichia coli had the smallest mean viable numbers at each stage of the digestion process. ...19938244898
in vitro activity of new quinoxaline compounds against campylobacter species and clostridium difficile.sc-44914 and sc-44942-a are two new quinoxaline compounds with a spectrum of activity similar to that of metronidazole. we studied the activity of sc-44914 and sc-44942-a against 35 campylobacter jejuni, 30 c. coli, and 20 clostridium (cl.) difficile and compared it with that of metronidazole by utilizing an agar dilution method. the quinoxalines had little activity against the c. jejuni and c. coli [minimum inhibitory concentration (mic)90 > or = 64 micrograms/ml]. sc-44914 and sc-44942-a had e ...19938243041
protection against campylobacter diarrhea: role of milk iga antibodies against bacterial surface antigens.in developing countries, campylobacter jejuni causes diarrhea and dysentery, especially in children less than one year of age. breast feeding protects against infectious diarrhea, with milk iga antibody playing a determining role. therefore, it has been proposed to increase the protective effect of human milk by vaccinating women of child-bearing age. to identify antigens which may induce protective breast-milk iga, we analyzed 60 strains of c. jejuni isolated from asymptomatically- and symptoma ...19938241641
chemical structures of the core regions of campylobacter jejuni serotypes o:1, o:4, o:23, and o:36 lipopolysaccharides.complete structures, including the location of n-acetylneuraminic acid (neu5ac) residues, were assigned for the core regions of campylobacter jejuni serotypes o:1, o:4, and o:23 and o:36 lipopolysaccharides (lps). in continuation of earlier studies, structure determinations of liberated oligosaccharides and, where necessary, of intact lps, were by 1h-nmr spectroscopy, smith degradation, chromium trioxide and enzymic degradations, in conjunction with methylation studies supported by fast-atom-bom ...19938240486
a case of bovine mastitis caused by campylobacter jejuni.a campylobacter jejuni mastitis in a holstein cow, 60 days into the first lactation, is reported. this was characterized by a sudden onset, a pyrexia, painful quarter and pink milk with a few small clots present. there was good response to a treatment protocol of: parenteral oxytetracycline, frequent stripping and intramammary infusions of erythromycin. this organism has a zoonotic property.19938237203
[spontaneous peritonitis caused by campylobacter jejuni]. 19938234991
a bacterium lipopolysaccharide that elicits guillain-barré syndrome has a gm1 ganglioside-like structure.there is a strong association between guillain-barré syndrome (gbs) and penner's serotype 19 (pen 19) of campylobacter jejuni. sera from patients with gbs after c. jejuni infection have autoantibodies to gm1 ganglioside in the acute phase of the illness. our previous work has suggested that gbs results from an immune response to cross-reactive antigen between lipopolysaccharide (lps) of the gram-negative bacterium and membrane components of peripheral nerves. to clarify the pathogenesis of gbs, ...19938228822
fine mapping of the three rrna operons on the updated genomic map of campylobacter jejuni tgh9011 (atcc 43431).the three rrna gene loci of campylobacter jejuni tgh9011 (atcc 43431) were cloned. all three rrna operons were shown to possess a contiguous 16s-23s structure and contain intercistronic trna(ala) and trna(ile). the three rna operons and additional 14 genetic markers were mapped in the updated genomic map of c. jejuni tgh9011, which now has a total of 24 genetic markers.19938226692
use of nonradioactive dna probes to identify a campylobacter jejuni strain causing abortion.a case of human abortion due to a campylobacter infection is reported. cultures revealed two morphologically different isolates with large and small colonies respectively. using conventional methods of identification, the large colonies were identified as campylobacter jejuni and the small colonies as campylobacter coli. dot blot hybridization and determination of rdna restriction fragment patterns revealed that both colony types were the same strain of campylobacter jejuni. this observation ill ...19938223663
plasmid profiles and antimicrobial susceptibility of campylobacter jejuni isolated from israeli children with diarrhea.thirty campylobacter jejuni (c. jejuni) strains isolated from stools of israeli children with enteritis were tested for sensitivity to eight antimicrobial agents (mic) and the presence of plasmids. it was found that all the isolates were sensitive to ciprofloxacin, ofloxacin, furazolidone and erythromycin. of the 30 strains tested, 21 (70%) were found to be tetracycline-resistant, a relatively high resistance rate as compared with data from other countries and previous reports from israel. plasm ...19938219507
vero cytotoxin-producing escherichia coli o157 infections in austria.to determine the prevalence of vero cytotoxin-producing escherichia coli (vtec) serotype o157 associated diarrhea in the austrian patient population, we surveyed all stool specimens of liquid consistency submitted to the federal public health laboratory (fphl) in innsbruck for 2 years for this organism. this laboratory serves a population of approximately 1 million people. of 5,265 stool specimens, 7 yielded o157 vtec. five isolates of e. coli o157 phage type 32, vt2 were cultured from specimens ...19938212755
monoclonal igm antibodies to gm1 and asialo-gm1 in chronic neuropathies cross-react with campylobacter jejuni lipopolysaccharides.we tested monoclonal igm anti-gm1 and asialo-gm1 antibodies from 6 patients with chronic motor neuropathies for binding to lipopolysaccharides (lps) from three stains of campylobacter jejuni. four of the 6 patients showed strong reactivity with lps from at least one of the three c. jejuni strains tested as shown by enzyme-linked immunosorbent assay or western blot. preabsorption with gm1 or asialo-gm1, or blocking with cholera toxin, prevented antibody binding to lps. these studies indicate that ...19948210226
[the role of flagella of campylobacter jejuni in colonization in the intestinal tract in mice and the cultured-cell infectivity].for analyzing the role of the bacterial flagella in colonization in the intestinal tract of mice and adhering to or invading the intestine 407 cell, a nonflagellated, nonmotile mutant was induced by ultraviolet irradiation of a flagellated, motile wild-type strain of campylobacter jejuni cf84-340. there was no great difference in the cellular infectivity to the intestine 407 cells between the wild-type and the mutant strains. cellular adherence and invasiveness were then compared by fluorescent ...19948201772
campylobacter jejuni bacteremia and guillain-barré syndrome in a patient with gvhd after allogeneic bmt.guillain-barré syndrome is a rare neurologic complication after allogeneic bmt. in the non-transplant setting, guillain-barré syndrome has typically been associated with antecedent acute infections and numerous reports have suggested an association between campylobacter jejuni infection and the subsequent development of guillain-barré syndrome. thus far, however, reports of c. jejuni-associated guillain-barré syndrome have been limited to gastrointestinal c. jejuni infections and none has been r ...19948199580
gm 1 antibodies in guillain-barré syndrome: isotypes, course and clinical outcome.iga, igg, and igm antibodies against ganglioside gm 1 were investigated in sera of 15 patients with guillain-barré syndrome (gbs), as compared with healthy controls. significantly raised titers were found in 4 patients who showed persisting weakness due to muscle wasting (3 patients) or due to the development of a chronic relapsing form of gbs (1 patient) after a follow up period of 1 year. by contrast, only 1 out of 11 anti-gm 1 seronegative patients showed persisting muscle wasting. in 1 patie ...19948197746
molecular typing of thermotolerant species of campylobacter with ribosomal rna gene patterns.ribosomal rna gene restriction patterns (ribopatterns) of 72 strains representing campylobacter jejuni (subspecies jejuni and doylei), c. coli, c. upsaliensis and c. lari including the urealytic (uptc) biovar were determined using four common restriction endonucleases (haeiii, hindiii, psti and pvuii). the relative effectiveness of these enzymes for general molecular typing of the thermotolerant campylobacters was assessed. ribotypes (haeiii) were defined on the basis of computer-assisted numeri ...19938190997
pcr-mediated dna typing of campylobacter jejuni isolated from patients with recurrent infections.the polymerase chain reaction (pcr) and two primers aiming at the enterobacterial repetitive intergenic consensus (eric) and arbitrary dna sequences, respectively, were used to fingerprint the genomic dna of 24 campylobacter jejuni strains isolated from five patients with recurrent c. jejuni infections. results were compared with biotyping and serotyping. the latter two methods, when combined, distinguished 9 different types, whereas pcr-mediated dna analysis discriminated 14 different patterns. ...19938190996
epidemiology of acute diarrheal diseases in children in a high standard of living rural settlement in israel.epidemiologic patterns of acute diarrheal diseases in a high standard of living communal settlement, situated in a region endemic for enteric diseases, were evaluated by a historical prospective study of 284 children (12,064 child months) from august, 1988, through july, 1992. three hundred eighty-three episodes of acute diarrhea were identified, yielding a rate of 0.38 episode per 12 child months. one hundred and children (35.6%) were reported to have 1 to 4 diarrheal episodes and 29 (10.2%) ch ...19948190536
aetiology of gastroenteritis at a major referral centre in saudi arabia.to determine the causes of gastroenteritis at a major referral centre in saudi arabia, retrospective study was carried out on 58,110 fresh stools from 42,035 patients. examination of stool specimens for pathogens was based on the clinical judgement of the physician responsible, so that all specimens were not tested for the presence of all pathogen groups. bacterial enteropathogens were found in 7.7% of patients; salmonella species (51.7%) were found to be the most frequent pathogens followed by ...19948187944
guillain-barré syndrome associated with igm anti-gm1 antibody following campylobacter jejuni enteritis.we report a 4-year-old girl diagnosed as having guillain-barré syndrome after infection by penner serotype 19 of campylobacter jejuni. the patient had the hla-b35 antigen. neurological examination revealed distal-dominant weakness and intact sensation. serial electrophysiological studies indicated that the predominant process was axonal degeneration involving motor nerves. an enzyme-linked immunosorbent assay revealed the presence of high titres of serum igm antibodies to gangliosides gm1 and gm ...19948181502
mucosal competitive exclusion to diminish colonization of chickens by campylobacter jejuni.mucosal competitive exclusion flora (mce) and length of mce culture storage were studied to determine their influence upon colonization of campylobacter jejuni in chickens. day-of-hatch broiler chicks, held in isolation units, were treated with mce or were left untreated. thirty to 48 h later, groups of chicks were challenged with serial dilutions of c. jejuni (strain a74/c). single challenge doses and treatments were administered to chickens within each isolation unit. after 5 d, chicks were ki ...19948177818
infection, diarrhea, and dysentery caused by shigella species and campylobacter jejuni among guatemalan rural children.to examine the factors that may influence the outcome of infections by shigella spp. and campylobacter jejuni we followed for 24 consecutive months 321 rural guatemala children 0 to 35 months old. home visits were made to determine child morbidity patterns with emphasis on diarrhea and dysentery. fecal samples for microbiologic studies were obtained from the participants when they were ill and during healthy periods. shigella spp. were isolated from 9.8 and 4.0% of ill and healthy children, resp ...19948177630
campylobacter jejuni, human immunodeficiency virus, and the guillain-barré syndrome. 19948169419
lipopolysaccharides from campylobacter jejuni associated with guillain-barré syndrome patients mimic human gangliosides in structure.lipopolysaccharides extracted from campylobacter jejuni serostrains (serotype reference strains) for serotypes o:4 and o:19 were found to have core oligosaccharides with terminal structures resembling human gangliosides gm1 and gd1a. high-molecular-weight molecules that reflected the presence of o chains were shown in immunoblots to be immunologically specific for each serostrain. the o:19 antiserum also reacted strongly with core oligosaccharides of two isolates from patients with guillain-barr ...19948168981
iron-regulated proteins in outer membranes of campylobacter jejuni diarrhoea isolates and immune response to the proteins in patients.the outer membrane protein (omp) profiles from 8 campylobacter jejuni and 5 campylobacter coli fecal isolates grown under various conditions were compared by sds-page. the bacteria were grown under usual conditions, in iron-deficient medium (dip) and on iron-supplemented medium (fe). the omp profiles of most bacterial strains grown under usual conditions, or in the fe-supplemented medium, contained four major bands of approximately 31, 45, 63-66 and 97 kda, and in addition, a number of minor ban ...19948167428
bacteria in blood for transfusion. a review.to summarize reports of bacterial contamination of blood components for transfusion during this century, considering implicated microorganisms and patient outcomes, with identification and discussion of recommended methods to reduce or eliminate this problem.19948166585
campylobacter jejuni gastroenteritis and acute encephalopathy. 19948163842
monitoring of selected bacteria and fungi in sand and sea water along the tel aviv coast.monitoring sand and sea water of the mediterranean tel aviv beaches during 1989-1991 for bacterial quality showed varying data and no correlation between standard bacteria parameters (sbp) and potential pathogenic bacteria (ppb). an exceptional feature was observed in 1989 when faecal coliforms including escherichia coli and faecal streptococcus were less in sand than in sea water. these results may be attributed to the low turbulent activity of the sea in 1989, particularly in summer, compared ...19948159125
prevalence of fluoroquinolone resistance in clinical strains of campylobacter jejuni isolated in spain. 19948157566
immunoglobulin a antibodies directed against campylobacter jejuni flagellin present in breast-milk.we studied the relationship between iga anti-campylobacter flagellin antibodies in breast milk samples and protection of breastfed infants living in a rural mexican village from campylobacter infection. there were fewer episodes of campylobacter infection (symptomatic and asymptomatic combined) in infants breastfed with milk containing specific anti-flagellin antibodies (1.2/child/year, 95% ci 0.6-1.8) versus non-breastfed children (3.3/child/year, 95% ci 1.8-4.8; p < 0.01). infants breastfed wi ...19948150009
cloning, characterization, and nucleotide sequence analysis of the argh gene from campylobacter jejuni tgh9011 encoding argininosuccinate lyase.the complete structural gene for argininosuccinate lyase (argh) from campylobacter jejuni tgh9011 has been cloned into escherichia coli by complementation of an e. coli argh auxotrophic mutant. the gene has been subcloned for sequencing on a 4.1-kb dna segment and localized by the complementing activity of deletion mutants. the complete dna sequence of the c. jejuni argh gene was determined. the transcription start point for argh mrna was determined by primer extension analysis and found to be w ...19948144452
guillain-barre syndrome and campylobacter jejuni infection. a study on the etiological characteristics of guillain-barre syndrome in china.fecal culture for campylobacter jejuni with the method of skirrow and serum class specific antibodies (igg, igm, iga) against campylobacter jejuni using a solid phase enzyme linked immunosorbent assay were performed in 17 cases of guillain-barre syndrome, 17 other neurological disease controls, and 33 normal controls. the results revealed: 1. the incidence of campylobacter jejuni infection, especially the recent one in guillain-barre syndrome was much higher than in the other two groups; 2. the ...19938143497
[isolation of campylobacter jejuni atcc 29428 from inoculated fried pork meat and roasted chicken].the human gastroenteritis caused by campylobacter jejuni in some industrialized countries is higher than gastroenteritis produced by salmonella and shigella. this has induced the development of techniques to demonstrate the presence of the microorganism in different foods using some culture media combinations. there is not a method to isolate c. jejuni from roasted chicken and fried pork meat, which are popular foods in méxico. the sensitivity of two culture media combinations was compared: rama ...19938140332
campylobacter jejuni: specific oligonucleotides and dna probes for use in polymerase chain reaction-based diagnosis.a 1189 base-pair long dna fragment, vs1, was isolated from a campylobacter jejuni cip 70.2 cosmid library and was found to contain regions specific for this bacterial species. for detection and identification of c. jejuni, two oligonucleotides derived from the vs1 sequence were used as primers in polymerase chain reaction tests on genomic dnas from 38 campylobacter and from 10 non-campylobacter strains. a specific, 358 base-pair long dna fragment was amplified only when c. jejuni dna was used as ...19938136783
acute diarrhoea and campylobacter in peruvian children: a clinical and epidemiologic approach.a study performed on peruvian children < 10 years of age and their controls showed that campylobacter jejuni is a leading cause of diarrhoea. infants 1 year old or younger were the most affected. the disease was usually moderately severe, although > 50 per cent of patients required i.v. fluid therapy. campylobacter was also more frequent in malnourished patients, who come from socio-economically deprived groups, with overcrowding, poor sanitation, and frequent contact with domestic birds and ani ...19938133554
etiology of diarrhea in a rural community in western thailand: importance of enteric viruses and enterovirulent escherichia coli.the etiology of gastroenteritis was determined in children and adults with diarrhea seen at a district hospital and three government health clinics in suan phung, western thailand, in 1991. enteric viruses (rotavirus and astrovirus) were identified in 40%, shigellae in 18%, attaching and effacing escherichia coli in 13%, campylobacter jejuni in 9%, and enterotoxigenic e. coli in 7% of children < 5 years old with diarrhea seen at the hospital. enteric viruses were detected in 15% (24/156) of pati ...19948133110
bacteraemia caused by campylobacter spp.the genus campylobacter has become increasingly recognised as the cause of various infections. campylobacter jejuni and c coli cause acute gastroenteritis in man all over the world. c jejuni enteritis can lead to bacteraemia, but its actual incidence remains unknown. seven cases of bacteraemia caused by c jejuni or c coli are reported, from the blood of seven patients: five immune deficient adults; a newborn baby; and a patient who had had abdominal surgery. patients who develop diarrhoea as a r ...19948132835
food and animal sources of human campylobacter jejuni infection. 19948125823
application of a dna-dna hybridization method for detection of campylobacter jejuni in chicken feces.a direct colony hybridization method was used for the detection of campylobacter jejuni in chicken feces. the biotin-labeled dna prepared from the whole genome dna of c. jejuni subsp. jejuni atcc 33560 reacted well with homologous dna and slightly with c. coli dna. the method with the probe was found to be sensitive enough to detect a small number (10(2) cfu/g) of c. jejuni in chicken feces which contained a large number of background flora. it was suggested that this simple and sensitive method ...19938117799
nursing disease in mink: clinical and postmortem findings.one hundred fifty lactating mink on one ranch in southern ontario were monitored over the lactation period in 1989 for evidence of clinical disease, and serial blood samples were collected for biochemical analysis. antemortem blood samples were collected and necropsies performed on the 17 females that developed nursing disease and on 17 controls matched to the same stage of lactation and on ten nonlactating controls. twenty-two additional nursing disease cases were selected from seven ranches in ...19938116144
foodborne bacterial infections in individuals with the human immunodeficiency virus.the literature contains reports documenting a foodborne etiology for bacterial infections caused by salmonella spp, listeria monocytogenes, campylobacter jejuni, and vibrio spp in individuals with the human immunodeficiency virus (hiv). the incidence of these infections and the life-threatening complications that result are elevated in people with hiv infection. we present practical recommendations to prevent foodborne illnesses and the resulting complications, including gastroenteritis, bactere ...19948115877
azithromycin resistance in campylobacter jejuni and campylobacter coli.the mics of erythromycin, azithromycin and ciprofloxacin were determined for 60 human fecal isolates of campylobacter. of these, 30 strains selected on the basis of their resistance to erythromycin by disk diffusion were highly resistant to both erythromycin and azithromycin. nine of these selected isolates were resistant to ciprofloxacin. the remaining 30 strains were non-selected, consecutive isolates of campylobacter susceptible to erythromycin by disk diffusion and were shown to be two- to f ...19938112360
modulation of mucosal immunity against campylobacter jejuni by orally administered cytokines.the effect of oral recombinant interleukin (ril) treatment on the course of campylobacter jejuni infection and the development of mucosal immunity in mice was investigated. ril-2, ril-5, and ril-6 were administered to mice at 24 and 6 h before infection and at 0, 24, and 48 h after infection with c. jejuni hc, and the subsequent development of an immune response and intestinal colonization resistance were determined. in this model, orally administered cytokines retained their biological activiti ...19938109936
effect of mutational alteration of asn-128 in the putative gtp-binding domain of tetracycline resistance determinant tet(o) from campylobacter jejuni.the deduced amino acid sequence of campylobacter jejuni tet(o), cloned in escherichia coli, has shown that it contains the five highly conserved sequences of the gtp-binding domain found in other gtpases. asn-128 belongs to the g4 motif of such a domain and is involved in hydrogen bonding with the guanine ring of the nucleotide. substitution of asn-128 by 11 other amino acids resulted in a decrease in tetracycline resistance, indicating that tetracycline resistance conferred by tet(o) is related ...19938109930
campylobacter jejuni and guillain-barré syndrome. 19948109911
the clinical correlates of high-titer igg anti-gm1 antibodies.serum igg anti-gm1 antibodies have been reported to occur in a variety of disorders, including guillain-barré syndrome and chronic polyneuropathies. of over 5,000 serums tested in our laboratory, high titers of selective igg anti-gm1 antibodies (> 1:1,000) and without binding to sulfatide were found in 35 patients. clinical correlation revealed that almost all patients had axonal, motor neuropathies. one subgroup was comprised of individuals with an acute motor neuropathy, described either as an ...19948109905
polymerase chain reaction-mediated dna fingerprinting for epidemiological studies on campylobacter spp.the applicability of polymerase chain reaction (pcr)-mediated dna typing, with primers complementary to dispersed repetitive dna sequences and arbitrarily chosen dna motifs, to study the epidemiology of campylobacter infection was evaluated. with a single pcr reaction and simple gel electrophoresis, strain-specific dna banding patterns were observed for campylobacter jejuni and c. upsaliensis. dna from multiple strains isolated during an outbreak of c. jejuni meningitis generated identical bandi ...19948107063
flagellin gene typing of campylobacter jejuni by restriction fragment length polymorphism analysis.we developed and studied a molecular typing approach for campylobacter spp. with restriction fragment length polymorphism (rflp) analysis of the flagellin gene flaa in c. jejuni. using polymerase chain reaction, we amplified the flaa gene from strains comprising different hl:o serotypes by using a primer set directed at the conserved 5' and 3' flaa gene sequence to generate a 1.7-kb amplicon. the amplicon was further digested with the restriction enzyme ddei, and the fragments generated were ana ...19938100241
distribution and polymorphism of the flagellin genes from isolates of campylobacter coli and campylobacter jejuni.the complex flagellar filaments of the lio8 serogroup member campylobacter coli vc167 are composed of two highly related subunit proteins encoded by the flaa and flab genes which share 92% identity. using oligonucleotide primers based on the known dna sequence of both the flaa and flab genes from c. coli vc167 in the polymerase chain reaction, we have shown conservation of both fla genes among isolates within the lio8 heat-labile serogroup by digestion of the amplified product with psti and ecor ...19938098328
oral immunoglobulin treatment in campylobacter jejuni enteritis. 19938096934
oral immunoglobulin treatment in campylobacter jejuni enteritis. 19938096933
rheumatic manifestations of campylobacter jejuni and c. fetus infections in adults.reports of the rheumatic manifestations of campylobacter jejuni and c. fetus infections in adults are reviewed in order to determine the most common presentations and which individuals are at risk for rheumatic disease. relevant english-language articles were identified through a medline search and from bibliographies of identified articles. 105 articles were reviewed in detail. 29 cases of reactive arthritis or reiter's syndrome following campylobacter jejuni enteritis were identified. the knee ...19948091140
iron-responsive genetic regulation in campylobacter jejuni: cloning and characterization of a fur homolog.the fur protein of escherichia coli represses transcription from fur-responsive genes in an iron-dependent manner. we have demonstrated a fur-like iron-responsive genetic regulatory activity operating in campylobacter jejuni by using a chloramphenicol acetyl transferase reporter gene separated from its promoter by a synthetic fur-responsive operator. a fur-like gene has been cloned from c. jejuni by partial functional complementation of an e. coli fur mutation. sequence analysis has shown that, ...19948083178
characteristics of cytotoxin produced by campylobacter jejuni strains.the cytotoxic activity against cultured cells of nineteen clinically isolated strains of campylobacter jejuni was tested. these strains were found to have different profiles in cytotoxin and enterotoxin production, and the characteristics of cytotoxin were further investigated. the cytotoxin showed cell killing toxicity against cho and hela cells. vacuole formation was observed in the case of rat hepatocyte primary culture. treatment with trypsin at 80 degrees c for 30 min inactivated the cytoto ...19948078411
two-year study of endemic enteric pathogens associated with acute diarrhea in new caledonia.a longitudinal study of diarrheal disease among patients of all ages with acute diarrhea was carried out in new caledonia from january 1990 to december 1991. stool samples from 2,088 diarrheal patients were examined for parasites, rotavirus, and bacterial pathogens. potential sources of contamination (drinking water, seawater and bovine and porcine feces) were investigated. one or more enteric pathogens were identified in 41.8 and 40.6% of the persons with diarrhea, in 1990 and 1991, respectivel ...19948077399
simple extraction of campylobacter lipopolysaccharide and protein antigens and production of their antibodies in egg yolk.antigens were heat extracted from campylobacter jejuni (li04) and c. coli (li020) in the presence of ethylenediaminetetraacetate (edta) and were recovered in the supernatant of a low-speed centrifugation. the method is simpler, safer and more efficient in extracting lipopolysaccharide (lps) antigens than the hot phenol method. the extracted antigens (lps plus several proteins) elicited production of antigen-specific antibodies in the egg yolk of immunized hens. antibodies purified by polyethylen ...19948074971
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