Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| respiratory syncytial virus mrna coding assignments. | the polypeptide coding assignments for six of the respiratory syncytial virus-specific mrnas were determined by translation of the individual mrnas in vitro. the coding assignments of the rnas are as follows. rna band 1 is complex and can be separated into at least two components on the basis of electrophoretic mobility (molecular weights [mws] approximately equal to 0.21 x 10(6) and 0.31 x 10(6), respectively) that code for three polypeptides of 9.5, 11, and 14 kilodaltons (k). rna 2 (mw, 0.39 ... | 1983 | 6682456 |
| [hospital epidemic outbreak of respiratory syncytial virus]. | 1983 | 6676858 | |
| [investigation on the level of antibodies against respiratory syncytial virus in children in nanning city]. | 1983 | 6675846 | |
| a study of respiratory infections in the elderly to assess the role of respiratory syncytial virus. | a prospective study was performed from 1 december 1981 to 31 may 1982 in two departments of geriatric medicine in edinburgh. this yielded 159 cases of acute respiratory tract infection (rti). twelve of these were undoubtedly associated with respiratory syncytial virus (rsv), 14 with influenza a and 18 with influenza b (as established in each case by a fourfold or greater increase in antibody titre). eighty-five of the 159 patients with rti and rsv titres of 32 or greater. their significance is d ... | 1983 | 6663083 |
| respiratory syncytial virus. | 1983 | 6662671 | |
| enzyme-linked immunosorbent assay for serum antibody to bovine respiratory syncytial virus: comparison with complement-fixation and neutralization tests. | an indirect enzyme-linked immunosorbent assay (elisa) for the detection of serum antibody to bovine respiratory syncytial virus (brsv) was developed and compared with complement-fixation (cf) and viral neutralization (vn) tests. tissue culture-grown viral antigens were used in the tests. serum samples from various sources were compared, including serum samples from 10 calves which were infected experimentally with brsv by aerosol exposure. the elisa compared favorably with the vn test for detect ... | 1983 | 6660613 |
| modified immunoprecipitation procedure for the identification of human respiratory syncytial virus polypeptides. | when analysed by polyacrylamide gel electrophoresis, human respiratory syncytial virus harvested after a one step growth cycle and purified through a continuous sucrose density gradient was shown to be composed of nine structural proteins of 90, 68, 49, 42, 34, 28, 25, 19 an 13 kd. the 90, 49 and 19 kd polypeptides were identified as glycopolypeptides by glucosamine incorporation. a modified immunoprecipitation procedure confirmed the viral specificity of the 49, 42, 28, 25 and 19 kd polypeptide ... | 1983 | 6643642 |
| total serum ige levels in venezuelan schoolchildren. | seventy-eight disease-free children were evaluated by prist for total serum ige in order to establish the normal range for this immunoglobulin and assess its utility, in tropical climates, such as venezuela, as a diagnostic tool for clinical allergy. seventy-eight normals were selected from a group of 1053 children, aged 7-12 years from fourteen schools in caracas. exclusion from the normal pool was based on nationality, and on historical, clinical, and/or laboratory evidence of atopic and/or in ... | 1983 | 6640887 |
| respiratory syncytial virus glycoproteins. | the proteins of respiratory syncytial (rs) virus were analyzed by sds-polyacrylamide gel electrophoresis. eight virion structural proteins with molecular weights of 180,000, 89,000, 48,000, 42,000, 34,000, 28,000, 25,000, and 21,000 were identified. these proteins were given tentative designations of l (180,000), g (89,000), f1 (48,000), np (42,000), p (34,000), m (28,000), vp25 (25,000), and f2 (21,000). the 89,000-, 48,000-, and 21,000-dalton polypeptides were glycosylated and could be purifie ... | 1983 | 6636537 |
| indirect haemagglutination test for the detection and assay of antibody to bovine respiratory syncytial virus. | an indirect haemagglutination (iha) test was used for the rapid assay of antibody to bovine respiratory syncytial virus. antigens for the sensitisation of formalised tanned erythrocytes were prepared by treatment of virus infected cells with non-ionic detergent. a close serological relationship was shown by the iha test between the strain of bovine respiratory syncytial virus used and the a2 strain of human respiratory syncytial virus. the iha test was sensitive and reproducible. a linear correl ... | 1983 | 6636489 |
| serology for diagnosis and epizootiological studies of bovine respiratory syncytial virus infections. | the complement fixation test (cft) and the virus neutralisation test (vnt), performed as a plaque reduction test, were employed to measure antibodies to bovine respiratory syncytial virus. the cft with bovine sera was performed with supplementation of the complement factors in fresh guinea pig serum by an adequate amount of clq-factor of the bovine species. kinetics of maternally derived antibodies and the antibody response after spontaneous and experimental infections and after intramuscular va ... | 1983 | 6635341 |
| respiratory syncytial virus pneumonia in young calves: clinical and pathologic findings. | nine young calves given respiratory syncytial virus by a combined intranasal and intratracheal route developed a severe respiratory tract disease in which coughing, tachypnea, and hyperpnea were prominent clinical features. calves were euthanatized on postinoculation (initial) days (pid) 1 to 13. at necropsy, large areas of consolidation were present in the cranial, middle, accessory, and cranial parts of the caudal lung lobes of calves killed between pid 4 and 13. histopathologic examination re ... | 1983 | 6625318 |
| viral infections in clinical pertussis. | of 44 children with clinical pertussis 29 (66%) had serological evidence of concurrent infection with respiratory viruses or mycoplasma pneumoniae. the commonest superinfection was by mycoplasma (32%), followed by respiratory syncytial virus (27%) and adenoviruses (16%). the high prevalence of viral infections, many being multiple, supports the theory that pertussis predisposes to such infections or vice versa. | 1983 | 6623245 |
| cellular and humoral immunity in recurrent respiratory syncytial virus infections. | 1983 | 6622111 | |
| polymorphonuclear function and respiratory syncytial virus infections in children. | 1983 | 6618679 | |
| giant cell pneumonia due to respiratory syncytial virus. occurrence in severe combined immunodeficiency syndrome. | a 6-month-old male infant with a severe combined immunodeficiency syndrome was hospitalized for progressive respiratory distress. examination during hospitalization disclosed widespread pulmonary infiltrates that did not respond to intensive therapy. the patient died eight days after admission. autopsy disclosed pneumocystis carinii pneumonia and widespread giant cell pneumonia. respiratory syncytial virus (rsv) was grown from a lung specimen obtained at autopsy. specific immunofluorescent stain ... | 1984 | 6611144 |
| characterization of the human peripheral blood effector cells mediating antibody-dependent cell-mediated cytotoxicity against respiratory syncytial virus. | peripheral blood lymphocytes were separated into several subpopulations and evaluated for their ability to mediate antibody-dependent cell-mediated cytotoxicity (adcc) against respiratory syncytial virus (rsv)-infected hela cells. using erythrocyte rosetting methods, nylon wool filtration, and cytolysis with okt-3 monoclonal antibody, two lymphocyte subpopulations were shown to mediate rsv-adcc; non-t, non-b, and igg-fc receptor-bearing lymphocytes and e-rosetting cells with iggfc receptors (t g ... | 1983 | 6603313 |
| construction and characterization of cdna clones for four respiratory syncytial viral genes. | cytoplasmic poly(a)-containing rna from respiratory syncytial virus-infected cells was used as a template to synthesize oligo(dt)-primed cdnas. discrete size classes of single-stranded cdnas, resolved by alkali agarose gel electrophoresis, were used separately to construct double-stranded cdnas that were subsequently inserted into the plasmid vector pbr322 at the pst i site by means of oligo(dg)oligo(dc) tailing. after transfection of escherichia coli, recombinant plasmids were screened mostly b ... | 1983 | 6572388 |
| sequence analysis of the respiratory syncytial virus phosphoprotein gene. | a recombinant cdna plasmid (prsa3) containing an almost full-length copy of the mrna encoding respiratory syncytial virus phosphoprotein was identified in a cdna library prepared with mrna from respiratory syncytial virus-infected cells. the cdna insert was sequenced, and a protein of 27,150 daltons was deduced from the dna sequence. the protein is relatively acidic, containing two clusters of acidic amino acids, one in the middle of the molecule and the other at the c-terminus. it is devoid of ... | 1984 | 6548527 |
| identification of a tenth mrna of respiratory syncytial virus and assignment of polypeptides to the 10 viral genes. | nine mrnas, their cdna clones, and a genome transcriptional map have been reported previously for respiratory syncytial virus (p. l. collins and g. w. wertz, proc. natl. acad. sci. u.s.a. 80:3208-3212, 1983). we report here the identification of a 10th viral mrna, designated mrna 2b (molecular weight [mw] ca. 0.39 x 10(6)), that was detected by rna (northern) blot hybridization with cdna clones. analysis of a polycistronic readthrough transcript was used to deduce the position in the viral trans ... | 1984 | 6546401 |
| propagation of bovine respiratory syncytial virus in calf testicle microcarrier culture. | 1984 | 6543300 | |
| [demonstration of respiratory syncytial virus infection in calves by the complement fixation reaction]. | studied was tentatively the occurrence of a respiratory syncytial virus infection on some farms with records of the disease. investigations were carried out on the base of the complement-fixation test (cft) specially worked out and on the specific complement-fixing antigen obtained from a bovine respiratory syncytial virus (brsv). tested were several methods for the production of a complement-fixing antigen. most appropriate proved the one obtained by the extraction of an infected cell monolayer ... | 1984 | 6528480 |
| a preliminary serological survey of viral antibodies in peruvian sheep. | this study reports the sero-prevalence of viral infections in sheep in peru. serum samples were collected from 34 mature healthy rams located in 3 different geographic regions of the country (north, central and south). the sera were tested for antibodies to the following viruses: respiratory syncytial virus (rsv); parainfluenza 3 (pi-3) virus; bovine viral diarrhea/border disease (bvd/bd) virus; bovine herpesvirus 1 (bhv-1); bluetongue (bt) virus; ovine progressive pneumoniae (opp) virus; bovine ... | 1984 | 6528458 |
| the development of respiratory syncytial virus-specific immune complexes in nasopharyngeal secretions following natural infection. | sequential samples of nasopharyngeal secretions (nps) from 61 infants and children with respiratory syncytial virus (rsv) infection were examined for the presence of rsv-specific immune complexes in order to determine their possible role in recovery from or pathogenesis of rsv infection. immune complexes in nps were identified by the raji cell assay using indirect immunofluorescence and rsv antigen in these complexes was also detected by indirect immunofluorescence. rsv-specific immune complexes ... | 1984 | 6527374 |
| an outbreak of respiratory syncytial virus pneumonia in a nursing home for the elderly. | an outbreak of pneumonia and febrile respiratory illness took place in a los angeles county nursing home for the elderly in february and march of 1979. forty of 101 (40%) residents were affected. twenty-two (55%) had pneumonia and eight (20%) died. the outbreak extended over a period of 6 weeks. serological evidence implicated respiratory syncytial virus (rsv) as the causative agent. three patients had fourfold or greater rises in the titre of antibody to rsv, one had high titres in paired sampl ... | 1984 | 6527041 |
| [viral-microbial associations and the function of humoral factors of natural immunity in acute pneumonia patients]. | a total of 359 patients with acute pneumonia and 152 practically healthy subjects comprising the control group were examined. immunofluorescence was used to investigate nasopharyngeal washings for detecting antigens of influenza and parainfluenza viruses, respiratory-syncytial virus, adenoviruses, whereas serological studies according to the hemagglutination delay test with diagnostic agents for detecting influenza a1, a2, b, types 1, 2 and 3 parainfluenza, and the complement fixation test were ... | 1984 | 6523359 |
| outbreak of respiratory syncytial virus infection in a neonatal intensive care unit. | 1984 | 6519955 | |
| prevalence of antibodies to respiratory syncytial virus in various populations in israel. | a preliminary study on the prevalence of antibodies to respiratory syncytial virus (rsv) in individuals of various age-groups was carried out. in healthy infants aged 6 to 12 months, the prevalence was 27%. it increased to 37 and 54% in children aged 1 to 2 and 2 to 4 years, respectively. the prevalence reached 78% in healthy individuals who were more than 20 years old, including pregnant women. significant levels of specific antibodies were detected in 59% of children aged 6 to 12 months who we ... | 1984 | 6519945 |
| cellular immune response to infection with respiratory syncytial virus and influence of breast-feeding on the response. | virus-specific lymphocyte transformation (ltf) activity in vitro was examined in 78 infants with various forms of illness due to respiratory syncytial virus (rsv) infection. in 73 subjects with lower respiratory tract disease, significant ltf activity was often detected within one week after onset of initial symptoms, and responses characteristic for each clinical form were observed in the subsequent rise. thus, mean activity in subjects with tracheobronchitis increased gradually, with the maxim ... | 1984 | 6512509 |
| a comparison of three vaccines against respiratory syncytial virus in calves. | an inactivated vaccine against respiratory syncytial virus (rsv) was compared with two live vaccines. the inactivated (gc) vaccine consisted of glutaraldehyde-fixed bovine nasal mucosa cells persistently infected with rsv and emulsified with oil adjuvant. the live vaccines were a modified virus (mv) derived from a bovine strain of rsv and a temperature-sensitive mutant (ts-1) derived from a human strain. the gc vaccine was inoculated subcutaneously into 12 calves and the live vaccines intramuscu ... | 1984 | 6501875 |
| immune adherence hemagglutination test for detection of antibody to respiratory syncytial virus. brief report. | an immune adherence hemagglutination test was established for detection of antibodies to respiratory syncytial virus. the test was shown to be as specific as and much more sensitive than the neutralization and complement fixation tests. | 1984 | 6497660 |
| field trials of a bovine respiratory syncytial virus vaccine. | a vaccine against bovine respiratory syncytial virus (brsv: norden), a cause of pulmonary edema-emphysema and pneumonia in calves, was evaluated in field trials on western nebraska beef calves at branding, preweaning, weaning and postweaning. of calves with undifferentiated respiratory infection at weaning, 20.9% of 2-dose vaccinates (169/810) and 27.5% of 1-dose vaccinates (230/837) required treatment, vs 48.2% of unvaccinated controls (397/823). at postweaning, 2.4% of 2-dose vaccinates (13/54 ... | 1984 | 6493205 |
| transcriptional mapping of human respiratory syncytial virus. | a transcriptional map for human respiratory syncytial virus was determined by measuring the kinetics of viral gene inactivation in response to uv irradiation. monolayer cell cultures of respiratory syncytial virus-infected hep-2 cells were exposed to uv light, and residual viral rna synthesis was monitored both by gel electrophoresis and by hybridization to dot blots of cloned cdnas of the 10 known viral genes. target sizes for the 10 individual viral genes were calculated relative to the uv sen ... | 1984 | 6492254 |
| interaction of polymorphonuclear leukocytes and viruses in humans: adherence of polymorphonuclear leukocytes to respiratory syncytial virus-infected cells. | the nature of neutrophil-respiratory syncytial virus (rsv) interaction was investigated by assessing factors that influence neutrophil adherence to rsv-infected tissue culture monolayers. the adherence of neutrophils to infected cells was directly proportional to the degree of rsv replication as evidenced by infectious virus production, cytopathological changes, or viral antigen appearance. sixty-one percent of the neutrophils adhered to the rsv-infected cells as compared with 52.7% on noninfect ... | 1984 | 6481852 |
| broad-spectrum antiviral activity of adenosine analogues. | in recent years certain aliphatic and carbocyclic adenosine analogues have been developed which are of potential clinical importance as antiviral agents. this includes (s)-9-(2,3-dihydroxypropyl)adenine [(s)-dhpa] and carbocyclic 3-deazaadenosine (c-c3ado). (s)-dhpa and c-c3ado are remarkably similar in their antiviral spectrum in that they are particularly active against (-) rna viruses such as measles, para-influenza, respiratory syncytial virus, rabies virus, vesicular stomatitis virus and (+ ... | 1984 | 6476818 |
| long-term prospective study in children after respiratory syncytial virus infection. | we have prospectively evaluated for the past 8 years 29 children who were hospitalized during infancy with acute lower respiratory tract illness caused by respiratory syncytial virus (rsv). no differences in the prevalence of a family history of atopy or breast-feeding in these infants compared with controls were noted. however, a history of parental smoking was significantly associated with hospital admission for rsv lower respiratory tract disease. evidence of atopy, as defined by serum ige le ... | 1984 | 6470859 |
| use of the biotin-avidin system to study the specificity of antibodies against respiratory syncytial virus. | to characterize the specificity of monoclonal antibodies against respiratory syncytial virus, we developed a technique which combined the biotin-avidin system, immunoprecipitation, and transblot electrophoresis. the viral proteins were first biotinylated and then immunoprecipitated with a monoclonal antibody or respiratory syncytial virus immune serum, separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and electrophoretically transferred to nitrocellulose paper. the protein ... | 1984 | 6470104 |
| respiratory syncytial virus. ii. isolation and morphology of the glycoproteins. | treatment of respiratory syncytial (rs) virus with triton x-100 in solutions of low ionic strength solubilized not only glycoproteins but also some non-glycosylated proteins. rate zonal sedimentation of the solubilized materials resulted in separation of the glycoproteins from the other components, i.e. one fraction predominantly composed of two glycoproteins each with molecular weight (mol. wt.) of approximately 100,000 and 53,000 was obtained. electron microscopic observation of this fraction ... | 1980 | 6452027 |
| management of respiratory failure in infants with acute viral bronchiolitis. | fifteen infants with acute viral bronchiolitis required mechanical ventilation. infants were all aged less than 12 weeks and all had evidence of atelectasis or pneumonia on chest x-ray films. respiratory syncytial virus was identified by immunofluorescence in 14 of 15 patients. intubation and mechanical ventilation were initiated to reduce the work of breathing rather than to treat hypoxemia alone. additional therapy included continuous positive airway pressure or positive end-expiratory pressur ... | 1984 | 6437212 |
| respiratory viruses and sudden infant death. | viruses were shown to be present in the respiratory tract in 200 of 763 cases of the sudden infant death syndrome studied in the nine years 1974-82. epidemiological and pathological evidence suggested that the distribution of viruses in the sudden infant death syndrome differs between infants aged 3 months or less and those aged over 3 months: the incidence of detection of virus was 14% in the younger group compared with 39% in the older group. the distribution of the viruses in these two groups ... | 1984 | 6426615 |
| respiratory syncytial virus infection in the elderly. | 1984 | 6419871 | |
| respiratory syncytial virus infection in the elderly 1976-82. | 1983 | 6416524 | |
| impact of viral respiratory diseases on infants and young children in a rural and urban area of southern west virginia. | acute viral respiratory disease occurring in children residing in the community of huntington, west virginia (urban children) or in the hollows surrounding huntington (rural children) was evaluated from september 1978 through march 1980. cohorts of ambulatory children residing in each area were studied for the occurrence of mild to moderate respiratory disease. all children admitted to hospitals were evaluated for the occurrence of severe viral respiratory disease. respiratory secretions were ob ... | 1983 | 6404161 |
| [rapid diagnosis of respiratory syncytial virus in children with bronchiolitis by indirect immunofluorescence technic]. | 1984 | 6400166 | |
| suppression of respiratory syncytial virus infection in cotton rats by bis(5-amidino-2-benzimidazolyl)methane. | intraperitoneal administration of bis(5-amidino-2-benzimidazolyl)methane at well-tolerated daily doses of 25 mg/kg subsequent to challenge and for 3 days thereafter effected over a 1-log reduction in the amount of virus recovered from lungs of cotton rats inoculated intratracheally with respiratory syncytial virus. when animals were immunosuppressed to prolong virus shedding, the reduction in recovered virus achieved with a 7-day dosing schedule of bis(5-amidino-2-benzimidazolyl)methane exceeded ... | 1984 | 6393863 |
| antibiotic treatment of pneumonia and bronchiolitis. a prospective randomised study. | routine administration of antibiotics in the treatment of pneumonia and bronchiolitis in infants and small children was evaluated in an open randomised prospective trial. from 1979-82 136 children between the age of 1 month and 6 years were allocated to one of two treatment groups shortly after their admission to a paediatric ward. group a patients were to be given antibiotics but those in group b were not. none of the children had received antibiotics before hospital admission. a viral infectio ... | 1984 | 6391389 |
| an enzyme-linked immunosorbent assay (elisa) for igg and iga antibodies to respiratory syncytial virus in low dilutions of secretions of human serum and secretions. | an enzyme-linked immunosorbent assay (elisa) has been developed for titration of igg and iga antibodies to respiratory syncytial (rs) virus in low dilutions of human serum, colostrum, and nasopharyngeal secretions. previously the sensitivity of rs virus elisa on such specimens has been limited by nonspecific absorption of antibody, particularly iga, to crude antigen preparations. for igg antibody estimation in infant sera, this unwanted binding was reduced to workable levels by increasing the se ... | 1984 | 6389773 |
| bronchiolitis: a clinical review. | bronchiolitis is an acute viral infection primarily caused by respiratory syncytial virus, affecting children under 2 years of age with a peak prior to six months. the clinical picture results from an inflammatory process of the small bronchi and bronchioles. infants present with tachypnea and wheezing often accompanied by respiratory distress and hyperinflation. supportive care is essential, children respond slowly to hydration and careful observation. respiratory distress requires hospitalizat ... | 1983 | 6389670 |
| immune response to respiratory syncytial virus: prevention of syncytia formation by human serum during in vitro infection. | human serum specimens containing respiratory syncytial virus (rsv)-specific neutralizing antibody were found to prevent the formation of syncytia when applied to hep-2 tissue culture monolayers which had been infected with rsv 12 h previously. this was evidenced by the demonstration of rsv-infected cells without any syncytia formation in the monolayers treated with rsv antibody-positive serum. on the other hand, widespread syncytia formation was observed with antibody-negative control serum. the ... | 1984 | 6387611 |
| interaction of bovine respiratory syncytial virus and pasteurella haemolytica in the ovine lung. | the potential synergistic effect of bovine respiratory syncytial virus (rsv) and pasteurella haemolytica in the production of pneumonia after aerosol/intranasal infection of conventionally reared lambs was evaluated. a mild clinical response was observed in lambs given virus and/or bacteria. gross pulmonary lesions were seen in 3 of 6 lambs given rsv and then p haemolytica 3 or 6 days later, respectively (groups d and e), and in 1 lamb of 5 given virus and bacteria simultaneously (group g). gros ... | 1984 | 6383152 |
| pathogenesis of experimental bovine respiratory syncytial virus infection in sheep. | conventionally reared lambs, seronegative to bovine respiratory syncytial virus (rsv), were inoculated with bovine rsv by an aerosol route. a mild clinical response, characterized by transient fever at postinoculation day (pid) 4, occurred and was accompanied by serous oculonasal secretions in less than 50% of the infected lambs. a decrease in total wbc was observed on pid 6 and 11, with a reduction in the total numbers of lymphocytes. bovine rsv was recovered from nasal secretions of inoculated ... | 1984 | 6383151 |
| cell-mediated immune response in cattle to bovine respiratory syncytial virus. | cattle inoculated with bovine respiratory syncytial virus (brsv) were evaluated for the development of a cell-mediated immune response. results of the leukocyte migration-inhibition test under agarose and the delayed hypersensitivity test indicated that a cell-mediated immune response was elicited after intranasal inoculation of calves with brsv. migration inhibition in the leukocyte migration-inhibition test was detected by postinoculation day (pid) 5 and reached maximum inhibition on pid 21. i ... | 1984 | 6383149 |
| respiratory virus infection in hospitalized children in austria 1979-1982. diagnosis by immunofluorescence. | nasal secretions from 349 austrian children under six years of age who were hospitalized for respiratory illnesses were screened for the presence of respiratory syncytial virus (rsv), parainfluenza virus 1 and 3, adenovirus and influenza a virus over a period of four years by the immunofluorescence technique. 35% of the specimens were found to be positive for one of the five viruses investigated. rsv was detected in 31% of the nasal secretions and was thus the most frequently encountered causati ... | 1984 | 6381315 |
| isolation of pathogenic bacteria from the nasopharynx of children with respiratory syncytial virus infection. predictive value of chest roentgen examination and laboratory tests. | potentially pathogenic bacteria were isolated from the nasopharynx in 33/66 hospitalized infants and children with verified respiratory syncytial virus (rsv) infection. the value of chest roentgenograms and blood counts for the prediction of concomitant bacterial infection was evaluated. abnormal chest roentgenograms were found in 89% whether pathogenic bacteria were present or not. the most common finding was general hyperinflation of the lungs, with or without infiltrates. this occurred more f ... | 1984 | 6377479 |
| viral diagnoses using the rapid immunofluorescence technique and epidemiological implications of acute respiratory infections among children in different european countries. | from november 1978 to october 1981, a total of 7716 specimens of nasopharyngeal secretions were examined by the rapid immunofluorescence technique to determine the frequency of infections caused by the respiratory syncytial virus (rsv), influenza virus a, and parainfluenza viruses 1 and 3. the tests were carried out in six different virus laboratories located in newcastle upon tyne (england), copenhagen, oslo, stockholm, turku (finland), and vienna; laboratories in lisbon and paris participated ... | 1984 | 6375886 |
| use of immunofluorescence in the study of the pathogenesis of respiratory syncytial virus infection. | 1983 | 6372603 | |
| respiratory syncytial virus diagnosis. | 1984 | 6372231 | |
| [respiratory syncytial virus and influenza a virus infections in children. a simplified preparation of samples for rapid virus diagnosis]. | 1984 | 6369621 | |
| evidence for both respiratory syncytial virus and measles virus antigens in the osteoclasts of patients with paget's disease of bone. | recent ultrastructural and immunohistochemical evidence supports the hypothesis that paget's disease of bone is a slow viral infection of the paramyxoviridae family. conflicting evidence for the presence of respiratory syncytial virus (rsv), a pneumovirus, or measles, a morbillivirus, has been reported. by the indirect fluorescent antibody assay, four rsv antisera were compared with four measles antisera on serial sections of pagetic bone or replicate coverslips of cells from pagetic bone grown ... | 1984 | 6365389 |
| experimental pneumonia in gnotobiotic calves produced by respiratory syncytial virus. | a bovine isolate of respiratory syncytial virus (rsv), when inoculated intranasally into eight gnotobiotic calves produced significant macroscopic lesions of the lung (2-25% consolidation) but failed to produce any clinical signs of disease. the microscopic lesions comprised proliferative and exudative bronchiolitis with accompanying alveolar collapse and infiltration by mononuclear cells of the peribronchiolar tissue and alveolar walls. virus was recovered from the nasopharynx between days 2 an ... | 1984 | 6365144 |
| infection of gnotobiotic calves with a bovine and human isolate of respiratory syncytial virus. modification of the response by dexamethasone. | a bovine and a human strain of rsv both adapted to bovine cell culture, have been inoculated separately into 13 and 7 gnotobiotic calves respectively by 3 different methods. both strains infected calves and showed similar growth patterns. virus was recovered from the nasopharynx between one and 11 days with peak titres between 3 and 8 days following inoculation. with the exception of 4 calves treated with dexamethasone no clinical signs and only minimal macroscopic lesions of the lung were induc ... | 1984 | 6365036 |
| detection by elisa of iga and igm antibodies in secretion and igm antibodies in serum in primary lower respiratory syncytial virus infection. | twenty-six infants and children with primary lower rs virus infection, diagnosed by the detection of rs virus in nasopharyngeal secretion (nps) by use of immunofluorescent antibody (fa) technique, were studied with respect to the presence of iga and igm antibodies. samples of nps and serum obtained during the first 3-4 months following the beginning of illness, were investigated. employing a reverse elisa technique, we found igm antibodies in the acute, but not during the convalescent, phase of ... | 1984 | 6363623 |
| protection from respiratory syncytial virus infection in cotton rats by passive transfer of monoclonal antibodies. | the effects of passive administration of neutralizing monoclonal antibodies (mab) to respiratory syncytial virus glycoproteins (gp90 and vp70) was evaluated in cotton rats challenged with respiratory syncytial virus. animals injected with mab to vp70 had lower mean viral titers in lung tissues than did controls (log10 2.5 versus 5.4 pfu/g; p less than 0.001), as did cotton rats given mab to gp90 (log10 2.1 versus 5.0 pfu/g; p less than 0.001). fifty percent of animals given either mab had no det ... | 1984 | 6363300 |
| respiratory syncytial virus detection by immunofluorescence in nasal secretions with monoclonal antibodies against selected surface and internal proteins. | specimens containing respiratory tract epithelial cells from infants and children with acute respiratory disease were evaluated by using an indirect immunofluorescence technique with two specific respiratory syncytial virus monoclonal antibodies. one (rs/hn 13-1) was directed against a cell surface viral antigen, and the other (rs/hn 25-2) was directed against viral antigen present in large cytoplasmic inclusions. the same results on presence or absence of respiratory syncytial virus were obtain ... | 1983 | 6361054 |
| [asthmatic syndrome in respiratory syncytial virus infection associated with influenza in young children]. | 1983 | 6356010 | |
| ribavirin aerosol treatment of bronchiolitis associated with respiratory syncytial virus infection in infants. | in a double-blind study, bronchiolitis associated with respiratory syncytial virus infection in 12 randomly selected patients treated with ribavirin aerosol improved more rapidly than in 14 control patients given saline aerosol (p = .044, wilcoxon rank sum test, two-tailed). an estimated 10 mg of ribavirin per kilogram of body weight was administered in daily 12-hour treatments over a five-day period. respiratory syncytial virus disappeared from secretions at about the same rate in treated and c ... | 1983 | 6356005 |
| rapid immunoperoxidase assay for detection of respiratory syncytial virus in nasopharyngeal secretions. | samples of nasopharyngeal secretions obtained from 70 infants and young children with acute respiratory disease were examined for the presence of respiratory syncytial virus by immunoperoxidase assay (ipa). the ipa was compared with the immunofluorescence assay and with cell culture isolation. respiratory syncytial virus antigen-positive cells were detected by both ipa and immunofluorescence assay in 28 specimens; 25 samples were positive in cell culture. the agreement between virus isolation an ... | 1983 | 6355168 |
| enzymatic cleavage of a glycoprotein of respiratory syncytial virus. | the 75k glycoprotein of the a2 strain of respiratory syncytial virus was cleaved by digestion with trypsin or staphylococcus aureus protease v8. the fragments resulting from trypsin digestion were 40k and 29k; those from the staphylococcal protease were 49k and 37k. | 1983 | 6354136 |
| correlation of cytotoxic activity in lungs to recovery of normal and gamma-irradiated cotton rats from respiratory syncytial virus infection. | cotton rats (sigmodon hispidus) that were exposed to 300, 600, or 900 rads of gamma irradiation and inoculated intranasally 2 days later with respiratory syncytial virus (rsv) exhibited prolonged virus shedding and delayed humoral and cytotoxic immune responses compared with comparably inoculated nonirradiated control rats. in nonirradiated animals and in animals exposed to 300 and 600 rads, levels of virus declined and then disappeared from the lungs during the period in which cytotoxic activit ... | 1983 | 6354026 |
| experimental respiratory syncytial virus pneumonia in young calves: microbiologic and immunofluorescent findings. | young calves were inoculated with respiratory syncytial virus (rsv) intranasally or by a combined intranasal and intratracheal route and were killed between postinoculation (initial) days (pid) 1 and 14. viral antigens were detected by immunofluorescence in nasopharyngeal cells from calves killed between pid 2 and 10. evidence of infection of the trachea and lungs with rsv was obtained by immunofluorescence and virus isolation in calves inoculated by the combined route, but not in calves inocula ... | 1983 | 6354017 |
| rapid diagnosis of respiratory syncytial virus and influenza a virus infections by immunofluorescence: experience with a simplified procedure for the preparation of cell smears from nasopharyngeal secretions. | a simplified procedure for preparing direct smears of exfoliated cells from nasopharyngeal secretions is presented. the specimens were examined by the indirect immunofluorescence (if) technique for the presence of respiratory syncytial (rs) virus and influenza a virus antigens. specimens from 457 children hospitalised at twelve different hospital departments in norway were examined during the winter season 1980-81. rs virus was identified in samples from 139 children and influenza a virus from 2 ... | 1983 | 6353858 |
| mechanisms of immunity to respiratory syncytial virus in cotton rats. | active immunity and maternally transmitted passive immunity to respiratory syncytial virus (rsv) were studied in cotton rats. animals infected with respiratory syncytial virus developed complete resistance to pulmonary reinfection, which lasted at least 18 months. nasal resistance was of shorter duration and began to diminish in 8 months. pulmonary resistance was transferred by parabiosis, but nasal resistance was not. adoptive transfer studies with fractionated convalescent blood showed that se ... | 1983 | 6352505 |
| evidence for a viral etiology of paget's disease of bone. | ultrastructural studies of the bone cells in patients with paget's disease of bone have revealed the presence of nuclear and cytoplasmic inclusions that resemble the nucleocapsids of paramyxoviridae virus. immunocytologic studies of bone biopsy specimens and cultured bone cells have demonstrated positive responses with antisera against measles virus and/or respiratory syncytial virus. these observations suggest that paget's disease may be a slow viral infection of bone. the exact nature of the p ... | 1983 | 6349897 |
| rapid detection of respiratory syncytial virus with a monoclonal antibody. | we developed an indirect fluorescent-antibody test employing a mouse monoclonal antibody directed against the nucleoprotein of rsv for rapid detection of respiratory syncytial virus (rsv). this test demonstrated distinctive fluorescent inclusions in hep-2 cells infected with 24 rsv isolates collected during 6 previous years but not in cells infected with 13 other respiratory viruses. examination of nasal cells of 100 infants with acute respiratory illness showed that the indirect fluorescent-ant ... | 1983 | 6348077 |
| lung defenses against infection: a clinical correlation. | the current state of knowledge of lung defenses has been reviewed. first, mechanical factors such as aerodynamic filtration and mucociliary transport were considered. then, in general terms, the contributions of alveolar macrophages, neutrophils, lymphocytes, and immunoglobulins, and the roles of complement, antiproteases, lysozyme, and fibronectin were examined. interactions between these components may regulate their effect. finally, the responses to five specific microorganisms were reviewed ... | 1983 | 6346246 |
| monoclonal antibodies to respiratory syncytial virus proteins: identification of the fusion protein. | six monoclonal antibodies directed against respiratory syncytial virus proteins were produced. each was characterized by immunoprecipitation and indirect immunofluorescence. one was directed against the nucleocapsid protein. np 44, two were directed against a 37,000-dalton protein, two were directed against the major envelope glycoprotein, gp 90, and one was directed against the 70,000-dalton envelope protein, vp 70. indirect immunofluorescence stain patterns of infected hep-2 cells defined gp 9 ... | 1983 | 6345804 |
| the nosocomial spread of respiratory syncytial viral infections. | respiratory syncytial virus is a regular winter visitor that is highly contagious among persons of all ages. it is the major nosocomial pathogen on infant and toddler wards, and has recently been recognized as also causing appreciable nosocomial illness in the elderly. control of the spread of this virus has been difficult, but transmission appears to require close contact via large-particle aerosols or via fomites. environmental conditions affect the survival of the virus on varying surfaces an ... | 1983 | 6344761 |
| aerosolized ribavirin treatment of infants with respiratory syncytial viral infection. a randomized double-blind study. | we evaluated a new antiviral agent, ribavirin, in the treatment of infants hospitalized with lower-respiratory-tract disease from respiratory syncytial virus. ribavirin or placebo was administered to 33 infants in a double-blind manner by continuous aerosol for three to six days. seventeen infants were treated with placebo, and 16 with ribavirin. by the end of treatment, infants receiving ribavirin had significantly greater improvement in their overall score for severity of illness, in lower-res ... | 1983 | 6343860 |
| ribavirin treatment of experimental respiratory syncytial viral infection. a controlled double-blind study in young adults. | the effect of ribavirin aerosol on experimental respiratory syncytial virus (rsv) infection was evaluated in a double-blind controlled study of 16 young adult volunteers. two days after intranasal inoculation with rsv, half of the subjects were treated with a small-particle aerosol of ribavirin and half with placebo for a total of 12 hours each day for three days. seven of eight placebo-treated and six of eight ribavirin-treated subjects became infected. viral shedding was diminished in the riba ... | 1983 | 6341640 |
| cell-mediated cytotoxic responses in lungs of cotton rats infected with respiratory syncytial virus. | pneumonia induced in cotton rats (sigmodon hispidus) after inoculation of respiratory syncytial virus (rsv) was accompanied by the appearance of leukocytes in infected lungs. the number of these leukocytes increased until day 5 after inoculation when sixfold more leukocytes were recovered from infected lungs using transpleural lavage than were recovered from lungs of uninfected control animals. although macrophages and lymphocytes constituted approximately 65 and 25%, respectively, of the cells ... | 1983 | 6340570 |
| prevalence of bovine herpesvirus-1, bovine viral diarrhea, parainfluenza-3, goat respiratory syncytial, bovine leukemia, and bluetongue viral antibodies in sheep. | sera from healthy sheep were collected in january and march 1982 from flocks of sheep located in southwestern and southeastern louisiana. these sera were tested for bovine herpesvirus-1 (bhv-1), bovine viral diarrhea virus (bvdv), parainfluenza-3 (pi-3) virus, and goat respiratory syncytial virus (grsv) antibodies by microtitration virus-neutralization test. the sera were tested also for bovine leukemia virus (blv) and bluetongue virus (btv) antibodies by immunodiffusion tests. the number of flo ... | 1984 | 6329044 |
| respiratory virus infections and aeroallergens in acute bronchial asthma. | two hundred and fifty six attacks of acute bronchial asthma occurring in 169 children aged over 2 years were studied during a two year period. more attacks occurred during spring and autumn than at other times of the year. in 73 patients (29%) a respiratory virus infection was diagnosed, with the same seasonal variation as the asthmatic attacks. most of the virus infections were caused by rhinovirus (45%) and respiratory syncytial virus (19%). there was no significant correlation between asthmat ... | 1984 | 6326694 |
| brs virus, pi3 virus and bhv1 infections of young stock on self-contained dairy farms: epidemiological and clinical findings. | the role of bovine respiratory syncytial virus, parainfluenza type 3 virus and bovine herpesvirus 1 as disease agents in 28 groups of young cattle on 19 dairy farms which raised their own replacements was investigated. bovine respiratory syncytial virus infections occurred in 27, parainfluenza type 3 virus infections in all and bovine herpesvirus 1 infections in three of the 28 groups. some infections were accompanied by clinical signs while others were entirely subclinical. clinical respiratory ... | 1984 | 6326374 |
| a simultaneous outbreak of respiratory syncytial virus and parainfluenza virus type 3 in a newborn nursery. | over a 3-week period, 20 of 34 (59%) infants in a newborn nursery developed nosocomial viral respiratory tract disease. either respiratory syncytial virus (seven infants) or parainfluenza virus type 3 (five) or both (two) were demonstrated in respiratory secretions from 14 of the 20 symptomatic patients. symptoms in the 20 infants included rhinitis (15 infants), cough (14), apnea (eight), pulmonary infiltrates (seven), and fever (six). there were no differences in symptoms between children infec ... | 1984 | 6325655 |
| viruses associated with acute respiratory infections in children admitted to hospital in naples, 1979-82. | a survey of the virological and epidemiological features of acute respiratory diseases in children admitted to hospital in naples has been carried out; the results of three years of research are reported.between april 1979 and march 1982, 787 nasopharyngeal swabs were examined. there were 287 (36.5%) positive samples, with the highest isolation rate being found in children with bronchiolitis (39.5%).among the different viruses isolated, adenovirus was the most common (161 positive samples, 56%); ... | 1983 | 6325032 |
| a prospective study of chlamydial, mycoplasmal, and viral infections in a neonatal intensive care unit. | in a prospective study of non-bacterial infection in a neonatal intensive care unit in north west london, chlamydia trachomatis infection was identified in 4 of 280 babies (1.4%) and was the most common cause of neonatal ophthalmia. one of the four developed pneumonitis. ureaplasma urealyticum was found to colonise the nasopharynx in 53 of 235 babies (22.6%), with mycoplasma hominis present in 6 of 235 babies (2.6%). there was a statistically significant association between u urealyticum colonis ... | 1984 | 6322705 |
| treatment of respiratory viral infection in an immunodeficient infant with ribavirin aerosol. | an infant with severe combined immunodeficiency syndrome (scids) secondary to adenosine deaminase deficiency had pneumonitis and combined infection with respiratory syncytial virus (rsv) and parainfluenza virus type 3 (piv3). four separate courses of ribavirin were delivered by small-particle aerosol. the piv3 disappeared during the first course, and rsv disappeared after the fourth course on the 58th hospital day. neither virus returned during profound immunosuppression for bone marrow transpla ... | 1984 | 6322573 |
| bronchopulmonary obstruction in children with respiratory virus infections. | among 873 children hospitalized with confirmed respiratory viral infections, 492 suffered from bronchopulmonary obstruction. respiratory syncytial virus infections were dominant among the patients with bronchopulmonary obstruction (80%). the majority of patients with rhinovirus infections suffered from bronchopulmonary obstruction (57%). two main groups of children at risk from developing bronchopulmonary obstruction during respiratory virus infections were found: 1) infants, suffering from bron ... | 1984 | 6321218 |
| [viruses and asthma]. | more than one-third of infants with respiratory syncytial virus (rsv) bronchiolitis subsequently experience recurrent episodes of wheezing. in asthmatic children, viral infections frequently trigger asthmatic attacks. indeed, viruses induce immunological and respiratory functional disorders. as compared with non-wheezing rsv infected infants, wheezing infants with bronchiolitis or asthma due to rsv have: a) increased t-lymphocyte responsiveness to rsv antigen; b) more persistent ige bound to nas ... | 1983 | 6320377 |
| improvement of respiratory syncytial virus replication in actively growing hep-2 cells. | the growth of respiratory syncytial (rs) and parainfluenza type iii (pi3) viruses has been studied in actively growing versus relatively stationary hep-2 cells. there was no effect on pi3 virus growth. rs virus synthesis was stimulated from 20- to 60-fold by growth in actively growing cultures when the cell density was approximately 1/3 that of a confluent culture. this stimulation was manifested by a greater yield of virus per cell, more virus-specific mrna produced per cell, and a 50% decrease ... | 1983 | 6317708 |
| [contribution of direct immunofluorescence diagnosis to the epidemiology of acute viral respiratory infections in young children]. | rapid diagnosis by immunofluorescence (if) has enabled us to study the epidemiology of acute viral respiratory diseases in infants admitted to the chru at caen between january 1980 and december 1982. 809 nasal aspiration samples were examined. respiratory syncytial virus (rsv) was recovered in 36% of cases. these were observed during the four winter outbreaks which occurred during the study period and were extensive in 1980-1981 and 1982-1983. a virus was demonstrated in 13.5% of the 515 rsv neg ... | 1983 | 6316549 |
| effect of sucrose phosphate and sorbitol on infectivity of enveloped viruses during storage. | cytomegalovirus and varicella-zoster virus recovery from sucrose phosphate (0.2 m sp) and 70% sorbitol (sorbitol) was compared after storage at -70, 4, and 20 degrees c over time. recovery from 0.2 m sp was uniformly better. more tissue culture infective doses and infectious foci were recovered in cell monolayers inoculated with 0.2 m sp virus stocks as compared with viruses stored in 70% sorbitol. although both viruses were isolated from diluted fresh stocks (10(-1) through 10(-4], freezing dil ... | 1983 | 6313747 |
| amino acid sequence of human respiratory syncytial virus nucleocapsid protein. | amino acid sequence of the human respiratory syncytial (rs) virus nucleocapsid (nc) protein, deduced from the dna sequence of a recombinant plasmid, is presented. the cdna plasmid (prsb11) has 1412 bp of rs viral nc sequence and lacks six nucleotides of the 5' end of mrna. there is a single long open reading frame encoding 467 amino acids. this 51540 dal protein is rich in basic amino acids and has no homologies with other known viral capsid proteins. | 1983 | 6310521 |
| activation of oxidative and arachidonic acid metabolism in neutrophils by respiratory syncytial virus antibody complexes: possible role in disease. | the effect of respiratory syncytial virus (rsv) antibody complexes on the metabolism of human neutrophils was determined by examining the generation of luminol-dependent chemiluminescence, superoxide, and thromboxane b2. incubation of neutrophils with rsv antibody complexes resulted in a significant increase in the production of chemiluminescence. the increase in chemiluminescence appeared to be due to (1) active phagocytosis of rsv antibody complexes as evidenced by 70% inhibition with cytochal ... | 1983 | 6309990 |
| [chronic isolated brain lesions caused by respiratory viruses in children]. | combined study of 48 postmortem observations of cerebral lesions caused mainly by meningococcal infection in children was carried out. in 15 cases, isolated subclinical involvement of the brain (more frequently, of the vascular plexus and meninges) caused by influenza a viruses (8 cases), b (2 cases), adenoviruses (6), parainfluenza and respiratory syncytial virus (1 each), and chlamydia (2) was found. this was proved by the presence of the appropriate antigens in the brain revealed by immunoflu ... | 1983 | 6309121 |
| elimination of contaminating bovine viral diarrhea virus from bovine respiratory syncytial virus stock. | 1983 | 6306037 | |
| viral infections of the respiratory tract in hospitalized children. a study from oslo during a 90 months' period. | a diagnosis of 979 respiratory viral infections was made in hospitalized children. respiratory syncytial virus greatly out-numbered the other viruses: it caused 58% of the total virus infections and occurred in winter epidemics. influenza a and b virus occurred during late winter and spring, rhinovirus had a seasonal distribution towards spring and autumn, whereas adenovirus types 1, 2 and 5 had no distinct seasonal distribution. whereas respiratory syncytial virus were mainly associated with br ... | 1983 | 6305106 |
| croup: an 11-year study in a pediatric practice. | the etiology and epidemiology of croup were studied in a pediatric group practice over an 11-year period, 1964 to 1975. croup was diagnosed in 951 instances in 6,165 cases of lower respiratory tract infection (lri) studied. as census figures of the practice clientele were available, attack rates were calculated. the incidence of total lri was highest in the first year of life. in contrast, the attack rate for croup was highest in the second year of life; the rate declined gradually after that ag ... | 1983 | 6304611 |
| hotting-up the complement-fixation test. | a detailed investigation into the effect of modifying the incubation temperature of the complement-fixation (cf) test is described. for varicella-zoster virus cytomegalovirus and rubella virus, increasing the incubation temperature progressively increased the sensitivity of the cf test to reach a maximum at 15 degrees c, at which temperature the geometric mean titre of seropositive samples was significantly greater than that found at 4 degrees c. for these three viruses, each serum shown to cont ... | 1983 | 6296229 |
| microfilaments associated with paget's disease of bone: comparison with nucleocapsids of measles virus and respiratory syncytial virus. | the structural characteristics and intracellular disposition of microtubules present in osteoclasts in paget's disease of bone were compared with those of nucleocapsids in cells infected with measles virus and respiratory syncytial virus (rsv). the distribution of microtubules in pagetic osteoclasts closely paralleled that of measles nucleocapsids in monkey kidney cells, but the dimensions of the pagetic microtubules were significantly different from those of measles virus. in contrast, pagetic ... | 1982 | 6292129 |