Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| inactivation of escherichia coli o157: h7 and listeria monocytogenes by pr-26, a synthetic antibacterial peptide. | this study reports the antibacterial effect of pr-26, a synthetic peptide derived from the first 26 amino acid sequence of pr-39, an antimicrobial peptide isolated from porcine neutrophils. a three-strain mixture of escherichia coli o157:h7 or listeria monocytogenes of approximately 10(8) cfu was inoculated to a final concentration of 10(7) cfu/ml in 1% peptone water (ph 7.0), containing 50 or 75 microg/ml of pr-26, and incubated at 37 degrees c for 0, 6, 12, and 24 h; at 24 degrees c for 0, 12, ... | 2001 | 11770619 |
| a retrospective study of neonatal mortality in farmed elk. | despite the increasing importance of the canadian elk industry, the veterinary literature concerning diseases of elk is sparse, in particular for the neonatal period. this study summarizes necropsy findings in 111 farmed elk calves, up to 30 days of age, submitted to the diagnostic laboratory of the western college of veterinary medicine over a 9-year period (990 to 1998). causes of mortality fit into 3 categories: infectious disease, noninfectious disease, and undetermined. organisms causing di ... | 2001 | 11769617 |
| listeria spinal cord abscess responsive to trimethoprim-sulfamethoxazole monotherapy. | to describe an alternative antibiotic regimen for the treatment of central nervous system listeria monocytogenes infection. | 2001 | 11766781 |
| an approach towards public health and foodborne human listeriosis--the austrian listeria monitoring. | the institute for milk hygiene, milk technology and food science has launched a listeria monitoring for austrian cheese factories in 1988 which is nowadays a valuable tool to control the safety of cheese production. it is a means to qualify the proper hygienic conditions in the participating cheese plants. proper hygiene protects cheese plants from getting contaminated by l. monocytogenes. the preventive elimination of foodborne pathogens facilitates a thriving economical development of the dome ... | 2001 | 11766273 |
| food-borne listeria monocytogenes risk assessment. | listeria monocytogenes is ubiquitous in the environment and in food processing plants. consequently, foods are frequently contaminated. however, the occurrence rate of listeriosis is only about five cases per million people per year. listeriosis primarily strikes immunocompromised individuals, pregnant women and the elderly with a fatality rate of 20-25%. the fda is in the process of finishing a risk assessment that is being conducted as an initial step in reviewing its approach to maximizing th ... | 2001 | 11761122 |
| exposure of listeria monocytogenes within an epidemic caused by butter in finland. | data on the levels of bacteria and the amounts of food consumed in food-borne outbreaks provides an excellent opportunity to study the effects of exposure to listeria monocytogenes. between june 1998 and april 1999, an outbreak caused by l. monocytogenes serotype 3a in butter occurred in finland. the majority of the cases were immunocompromised and hospitalized at the helsinki university central hospital (huch), where 7-g butter packages produced by a dairy plant were used as the only butter bra ... | 2001 | 11759767 |
| prevalence and characteristics of shigatoxin-producing escherichia coli and listeria monocytogenes strains isolated from minced meat in switzerland. | a total of 400 minced meat samples from 240 small butcheries in switzerland were collected and analysed for the presence of shigatoxin-producing e. coli (stec) and listeria monocytogenes. the samples comprised 211 samples of minced beef and 189 samples of minced pork. shigatoxin-producing e. coli was isolated from 7/400 (1.75%) samples. in particular, 5/211 (2.3%) minced beef samples and 2/189 (1%) minced pork samples were contaminated. serotyping of the seven strains yielded five different sero ... | 2001 | 11759763 |
| occurrence of listeria monocytogenes in food in chile. | out of 2145 food samples analysed 77 were found contaminated with listeria monocytogenes in santiago, chile. samples were: 603 ice-cream (3.5% contaminated), 256 soft cheese (0.8%), 155 hard cheese (0%), 229 baby milk bottles (0%), 634 processed meat products (3.6%) and 268 crustaceous shellfish (11.6%). three different isolation media were used: for 318 samples, modified mcbride agar (mma), lithium chloride phenylethanol moxalactam agar, and polymyxin acriflavine lithium chloride ceftazidime ae ... | 2001 | 11759755 |
| the effect of inoculum size on the lag phase of listeria monocytogenes. | the effect of inoculum size on population lag times of listeria monocytogenes was investigated using the bioscreen automated microtitre plate incubator and reader. under optimum conditions, lag times were little affected by inoculum size and there was little variation between replicate inocula even at very low cell numbers. however, in media containing inhibitory concentrations of nacl, both the mean lag time and variation between replicate inocula increased as the inoculum size became smaller. ... | 2001 | 11759754 |
| validation of iso method 11290 part 2. enumeration of listeria monocytogenes in foods. | the european and international standard method for the enumeration of listeria monocytogenes, described in en iso 11290 part 2: 1998 [en iso 11290-2 microbiology of food and animal feedingstuffs-horizontal method for the detection and enumeration of l. monocytogenes: part 2. enumeration; international organisation for standardisation, geneva.] was validated by order of the european commission (standards, measurement and testing fourth framework programme project smt4-ct96-2098). the objective wa ... | 2001 | 11759750 |
| hpt, a bacterial homolog of the microsomal glucose- 6-phosphate translocase, mediates rapid intracellular proliferation in listeria. | efficient replication in vivo is essential for a microparasite to colonize its host and the understanding of the molecular mechanisms by which microbial pathogens grow within host tissues can lead to the discovery of novel therapies to treat infection. here we present evidence that the foodborne bacterial pathogen listeria monocytogenes, a facultative intracellular parasite, exploits hexose phosphates (hp) from the host cell as a source of carbon and energy to fuel fast intracellular growth. hp ... | 2002 | 11756655 |
| safety and immunogenicity in neonatal mice of a hyperattenuated listeria vaccine directed against human immunodeficiency virus. | cd8(+) t cells are a major component of the adaptive response of a host to infections by viruses and other intracellular pathogenic agents. however, because of the intrinsic immaturity of the immune system of neonatal animals, neonates are highly sensitive to a variety of pathogens and may be unable to respond in a protective manner. here we explore whether a hyperattenuated strain of listeria monocytogenes that can be used as a live vaccine vector in adults is safe and able to induce an effecti ... | 2002 | 11752181 |
| complementary adhesion molecules promote neutrophil-kupffer cell interaction and the elimination of bacteria taken up by the liver. | most bacteria that enter the bloodstream are taken up by the liver. previously, we reported that such organisms are initially bound extracellularly and subsequently killed by immigrating neutrophils, not kupffer cells as widely presumed in the literature. rather, the principal functions of kupffer cells demonstrated herein are to clear bacteria from the peripheral blood and to promote accumulation of bactericidal neutrophils at the principal site of microbial deposition in the liver, i.e., the k ... | 2002 | 11751975 |
| early translocation of acid-adapted listeria monocytogenes during enteric infection in tnf/ltalpha-/- mice. | tnf/ltalpha deficient mice are devoid of peyer's patches and lack mesenteric lymph nodes. translocation, especially in the early steps after intragastric delivery of listeria monocytogenes, has been explored in this study, and the role of tnfalpha has been addressed. we showed that l. monocytogenes translocation occurred at least as efficiently in tnf/ltalpha-/- mice as in tnf/ltalpha+/+ littermates. even very low inocula (2.7x10(4) cfu) could initiate infection in the tnf/ltalpha deficient mice ... | 2001 | 11750799 |
| immunomodulatory effects of dietary lipids alter host natural resistance of mice to listeria monocytogenes infection. | over the past two decades, unsaturated fatty acids have received particular attention due to their ability to suppress immune functions. nevertheless, suppression of immune functions also involves a reduction of host natural resistance to eliminate the infectious agents. we have analyzed the role of dietary lipids on immune functions in cells cultured with listeria monocytogenes. bactericidal efficiency of peritoneal cells from mice fed a fish oil diet against this bacterium was reduced and the ... | 2001 | 11750222 |
| congenital nemaline myopathy due to acta1-gene mutation and carnitine insufficiency: a case report. | a premature boy with a congenital form of nemaline myopathy due to mutation in the acta1-gene showed decreased carnitine levels in the eighth week of life. after sufficient oral carnitine substitution he improved gradually. in the first 15 months of life he made good progress; he reached full head control, learned to sit unsupported and was able to raise objects. at that time the carnitine levels were normal without substitution. nemaline myopathy is clinically and genetically heterogenous. the ... | 2001 | 11748499 |
| stk receptor tyrosine kinase regulates susceptibility to infection with listeria monocytogenes. | we have previously identified the stk receptor tyrosine kinase as a key regulator of macrophage activation and cell-mediated immune responses. here we demonstrate that, although msp activation of stk inhibits no production by macrophages in response to heat-killed listeria monocytogenes, stk-deficient mice exhibit increased susceptibility to infection with listeria. | 2002 | 11748211 |
| cd8(+)-t-cell response to secreted and nonsecreted antigens delivered by recombinant listeria monocytogenes during secondary infection. | understanding how existing antivector immunity impacts live vaccine delivery systems is critical when the same vector system may be used to deliver different antigens. we addressed the impact of antivector immunity, elicited by immunization with attenuated acta-deficient listeria monocytogenes, on the cd8(+)-t-cell response to a well-characterized lymphocytic choriomeningitis virus epitope, np118-126, delivered by infection with recombinant l. monocytogenes. challenges of immune mice with acta-d ... | 2002 | 11748177 |
| [listeria monocytogenes infections in the adult. clinical and microbiological issues of a changing disease]. | thirty-one cases of human listeriosis seen from 1971-1999 were reviewed. cases were grouped as follows: group i composed of 14 patients were studied in the period 1971-1984; and group ii composed of 17 cases studied in the period 1985-1999. we tried to assess changes in the incidence, clinical findings and outcome in both periods. the incidence of listeriosis remained constant along the years, 1.2 cases/20,000 discharges. the mean age of the patients significantly increased along the years (55 1 ... | 2001 | 11747787 |
| detection and identification of spotted fever group rickettsiae and ehrlichiae in african ticks. | rickettsia africae, a recently identified pathogen, was detected for the first time in amblyomma ticks from niger, mali, burundi, and sudan, and "r. mongolotimonae" was identified for the first time in africa. rickettsiae of unknown pathogenicity and two new ehrlichiae of the ehrlichia canis group were identified in ticks from mali and niger. | 2001 | 11747731 |
| effect of prevention measures on incidence of human listeriosis, france, 1987-1997. | to assess the impact of preventive measures by the food industry, we analyzed food monitoring data as well as trends in the incidence of listeriosis estimated through three independent sources: the national reference center of listeriosis; a laboratory-based active surveillance network; and two consecutive nationwide surveys of public hospital laboratories. from 1987 to 1997, the incidence of listeriosis decreased by an estimated 68%. a substantial reduction in the proportion of listeria monocyt ... | 2001 | 11747725 |
| puma-g, an ifn-gamma-inducible gene in macrophages is a novel member of the seven transmembrane spanning receptor superfamily. | ifn-gamma is a key immunoregulatory cytokine that plays a predominant role in innate immunity. by employing pcr-select to search for genes differentially expressed in ifn-gamma/tnf-alpha stimulated macrophages, we identified a novel ifn-gamma-induced transcript designated puma-g (protein up-regulated in macrophages by ifn-gamma). puma-g codes for a protein with seven transmembrane helices, a feature commonly shared with the g protein-coupled receptor superfamily (gpcr). the puma-g protein is mos ... | 2001 | 11745392 |
| antimicrobial and anti-lipase activity of quercetin and its c2-c16 3-o-acyl-esters. | neither quercetin (q), nor 3-o-acylquercetines, up to 100 microg/ml, had any significant activity on selected gram-positive strains (staphylococcus aureus, bacillus subtilis, listeria ivanovi, listeria monocytogenes, listeria serligeri), gram-negative strains (escherichia coli, shigella flexneri, shigella sonnei, salmonella enteritidis, salmonella tiphymurium) and yeasts (candida albicans and candida glabrata). in addition, we confirmed the known anti-hiv activity of q (80% inhibition at 40 micr ... | 2002 | 11741775 |
| a sigma(54)-dependent pts permease of the mannose family is responsible for sensitivity of listeria monocytogenes to mesentericin y105. | sensitivity of listeria monocytogenes to the bacteriocin mesentericin y105 was previously shown to be dependent on the sigma(54) subunit of the rna polymerase. this points towards expression of particular sigma(54)-dependent genes. the present study describes first, manr, a new sigma(54)-associated activator, and second, ell(t)(man), a new sigma(54)-dependent pts permease of the mannose family, both involved in sensitivity to mesentericin y105, since interruption of their corresponding genes led ... | 2001 | 11739758 |
| immunoproteasomes largely replace constitutive proteasomes during an antiviral and antibacterial immune response in the liver. | the proteasome is critically involved in the production of mhc class i-restricted t cell epitopes. proteasome activity and epitope production are altered by ifn-gamma treatment, which leads to a gradual replacement of constitutive proteasomes by immunoproteasomes in vitro. however, a quantitative analysis of changes in the steady state subunit composition of proteasomes during an immune response against viruses or bacteria in vivo has not been reported. here we show that the infection of mice wi ... | 2001 | 11739503 |
| endogenous serratia marcescens endophthalmitis with dark hypopyon: case report and review. | a case of endogenous serratia marcescens endophthalmitis in a patient with diabetes, end-stage renal disease, and an indwelling venous catheter is reported. the patient presented with a tan hypopyon and elevated intraocular pressure. diagnosis was established by positive blood, vitreous, conjunctival, and catheter tip cultures. after a deteriorating course the eye was enucleated. gross and histopathologic examination revealed the presence of a dark hypopyon with iris necrosis and pigment dispers ... | 2001 | 11738433 |
| synergy between the n- and c-terminal domains of inlb for efficient invasion of non-phagocytic cells by listeria monocytogenes. | inlb is a listeria monocytogenes protein promoting entry in non-phagocytic cells, and has been shown recently to activate the hepatocyte growth factor receptor (hgfr or met). the n-terminal domain of inlb (lrrs) binds and activates met, whereas the c-terminal domain of inlb (gw modules) mediates loose attachment of inlb to the listerial surface. as hgf activation of met is tightly controlled by glycosaminoglycans (gags), we tested if gags also modulate the met-inlb interactions. we show that inl ... | 2001 | 11737639 |
| a role for acta in epithelial cell invasion by listeria monocytogenes. | we assessed the role of the actin-polymerizing protein, acta, in host cell invasion by listeria monocytogenes. an in frame deltaacta mutant was constructed in a hyperinvasive strain of prfa* genotype, in which all genes of the prfa-dependent virulence regulon, including acta, are highly expressed in vitro. loss of acta production in prfa* bacteria reduced entry into caco-2, hela, mdck and vero epithelial cells to basal levels. reintroduction of acta into the deltaacta prfa* mutant fully restored ... | 2001 | 11736996 |
| bacterial meningitis: current controversies in approaches to treatment. | acute bacterial meningitis continues to be a disease with unacceptably high mortality and morbidity rates in both adults and children worldwide, despite advances in antibacterial therapy. death or permanent disability occurs frequently. the causative organism varies with age, immune function and immunisation status. infection with neisseria meningitidis, streptococcus pneumoniae and haemophilus influenzae type b (hib) is associated with the majority of cases, with listeria monocytogenes and stre ... | 2001 | 11735611 |
| rapid detection of listeria monocytogenes in foods, by a combination of pcr and dna probe. | listeria monocytogenes is a frequent contaminant of water and foods. its rapid detection is needed before some foods can be prepared for marketing. in this work l. monocytogenes has been searched for in foods, by a combination of polymerase chain reaction (pcr) and a dna probe. both pcr and the probe were prepared for recognizing a specific region of the internalin gene, which is responsible for the production of one of the most important pathogenic factors of listeria. the combined use of pcr a ... | 2001 | 11735299 |
| sample preparation methods for pcr detection of escherichia coli o157:h7, salmonella typhimurium, and listeria monocytogenes on beef chuck shoulder using a single enrichment medium. | to improve the utility of the polymerase chain reaction (pcr) for food samples, methods for preparing template dna were developed to remove pcr inhibitors. beef chuck shoulder medallions, artificially contaminated, individually or in combination, with escherichia coli serotype o157:h7 strain fsis 45753-35, salmonella typhimurium dt104 strain 13hp, or listeria monocytogenes strain scott a at concentrations of 10, 1 and 0.5 cfu/cm(2)were swabbed with a sponge, and the sponges were enriched for 18 ... | 2001 | 11735298 |
| [notification according to the law for protection against infections]. | since january 1st 2001, the law for protection against infections decrees the duty of notification along two tracks, on the one hand by notification of infectious diseases and on the other hand by reporting the pathogens. however, the names of pathogens change due to scientific progress. furthermore, these names include in some cases not only harmful agents of infectious diseases but also inoffensive organisms, i. e. giardia lamblia. there are also problems in the notification of other pathogens ... | 2001 | 11735068 |
| first report of an outbreak of ovine septicaemic listeriosis in saudi arabia. | although a case of human listeriosis has recently been reported in saudi arabia, the disease has not been reported in animals to date. the authors describe an outbreak of septicaemic listeriosis in sheep, which occurred during winter. adult animals and pregnant ewes were principally affected, with a morbidity rate of 7.1% and a mortality rate of 2.4%; no abortions were recorded during the outbreak. clinical signs included inappetence, weakness, fever, respiratory distress, keratoconjunctivitis a ... | 2001 | 11732420 |
| actin-based motility of intracellular microbial pathogens. | a diverse group of intracellular microorganisms, including listeria monocytogenes, shigella spp., rickettsia spp., and vaccinia virus, utilize actin-based motility to move within and spread between mammalian host cells. these organisms have in common a pathogenic life cycle that involves a stage within the cytoplasm of mammalian host cells. within the cytoplasm of host cells, these organisms activate components of the cellular actin assembly machinery to induce the formation of actin tails on th ... | 2001 | 11729265 |
| lifestyles of the intracellular. | 2001 | 11728864 | |
| microbial genomics. dynamic duos. | 2001 | 11728863 | |
| characterization of anti-listeria monocytogenes bacteriocins from enterococcus faecalis, enterococcus faecium, and lactococcus lactis strains isolated from raïb, a moroccan traditional fermented milk. | seventy-four samples of raïb, a moroccan traditional fermented milk, were screened for their anti-listeria monocytogenes activity. nine lactic acid bacteria with antilisterial activity were isolated and identified as lactococcus lactis [4], enterococcus faecium [4], and e. faecalis [1]. antibacterial spectra, determined against 45 target strains, led to the selection of four antibacterial-producing strains, which were further characterized. their anti-microbial agents, inactivated by one or more ... | 2002 | 11727035 |
| evaluation of universal preenrichment broth for growth of heat-injured pathogens. | universal preenrichment broth (upb) was developed to enable enrichment of injured foodborne pathogens of different genera simultaneously in lieu of having to undergo separate simultaneous enrichment cultures for subsequent detection or isolation of each pathogen. enrichment conditions in upb for growth of injured pathogens to populations that will enable pathogen detection by rapid immuno-based or polymerase chain reaction (pcr)-based assays have not been defined. hence, studies were done to det ... | 2001 | 11726154 |
| combination of immunomagnetic separation and polymerase chain reaction for the simultaneous detection of listeria monocytogenes and salmonella spp. in food samples. | a method that combined the immunomagnetic separation (ims) technique and the multiplex polymerase chain reaction (pcr) method (i.e., the ims-mpcr method) was developed for simultaneous detection of listreria monocytogenes and salmonella spp. in food samples. when only the multiplex pcr method was used, it was found that if cell numbers of each of the two target organisms (l. monocytogenes and salmonella spp.) were above the detection limit, but differed by more than 2 logs-e.g., n x 10(7) to n x ... | 2001 | 11726153 |
| detection of heat injury in listeria monocytogenes scott a. | methods of detecting live pathogens in foods that may be growth inhibited following heat treatment are essential to food safety. among the techniques available, reverse transcription polymerase chain reaction (rt-pcr) amplification of messenger rna from heat-injured listeria monocytogenes scott a is preferable to direct pcr in an attempt to avoid false positives from dead cells. the rt-pcr has a detection limit of 3 x 10(6) cfu/g, compared to 3 cfu/g for untreated controls, but may not be suitab ... | 2001 | 11726152 |
| reduction of listeria monocytogenes on green peppers (capsicum annuum l.) by gaseous and aqueous chlorine dioxide and water washing and its growth at 7 degrees c. | reduction of listeria monocytogenes scott a on uninjured and injured surfaces of green peppers after 0.3- and 3-mg/ liter gaseous and aqueous clo2 treatment and water washing for 10 min at 20 degrees c was studied. growth of the l. monocytogenes untreated or treated with 0.6 mg/liter clo2 gas for 30 min at 20 degrees c on green peppers also was investigated. a membrane-surface-plating method was used for resuscitation and enumeration of l monocytogenes treated with clo2. the bacterial viability ... | 2001 | 11726151 |
| organic acids and their salts as dipping solutions to control listeria monocytogenes inoculated following processing of sliced pork bologna stored at 4 degrees c in vacuum packages. | postprocessing contamination of cured meats with listeria monocytogenes has become a major concern for the meat processing industry and an important food safety issue. this study evaluated aqueous dipping solutions of organic acids (2.5 or 5% lactic or acetic acid) or salts (2.5 or 5% sodium acetate or sodium diacetate, 5 or 10% sodium lactate, 5% potassium sorbate or potassium benzoate) to control l. monocytogenes on sliced, vacuum-packaged bologna stored at 4 degrees c for up to 120 days. orga ... | 2001 | 11726150 |
| validation of apple cider pasteurization treatments against escherichia coli o157:h7, salmonella, and listeria monocytogenes. | time and temperature pasteurization conditions common in the wisconsin cider industry were validated using a six-strain cocktail of escherichia coli o157:h7 and acid-adapted e. coli o157:h7 in ph- and degrees brix-adjusted apple cider. strains employed were linked to outbreaks (atcc 43894 and 43895, c7927, and usda-fsis-380-94) or strains engineered to contain the gene for green fluorescent protein (pgfp atcc 43894 and pgfp atcc 43889) for differential enumeration. survival of salmonella spp. (c ... | 2001 | 11726144 |
| molecular typing by pulsed-field gel electrophoresis of spanish animal and human listeria monocytogenes isolates. | a total of 153 strains of listeria monocytogenes isolated from different sources (72 from sheep, 12 from cattle, 18 from feedstuffs, and 51 from humans) in spain from 1989 to 2000 were characterized by pulsed-field gel electrophoresis. the strains of l. monocytogenes displayed 55 pulsotypes. the 84 animal, 51 human, and 18 feedstuff strains displayed 31, 29, and 7 different pulsotypes, respectively, indicating a great genetic diversity among the spanish l. monocytogenes isolates studied. l. mono ... | 2001 | 11722943 |
| listeria keratitis in a horse. | listeria monocytogenes is ubiquitous in the environment but is rarely reported as a cause of keratitis in animals. in this case, a mare was presented with epiphora and evidence of pain in the right eye. listeria monocytogenes was isolated from a corneal lesion, and bacteria were also seen in the cytologic evaluation. this is the first reported case of ulcerative keratitis associated with l. monocytogenes in a horse. | 2001 | 11722787 |
| molecular epidemiology of listeria monocytogenes isolates collected from the environment, raw meat and raw products in two poultry- and pork-processing plants. | in order to study the transmission of listeria monocytogenes in a poultry and a pork meat plant, we analysed the contamination by this pathogen over several months. | 2001 | 11722667 |
| adsorption of biosurfactant on solid surfaces and consequences regarding the bioadhesion of listeria monocytogenes lo28. | the influence of biosurfactant compounds produced by a strain of pseudomonas fluorescens on the adhesion of listeria monocytogenes lo28 to polytetrafluoroethylene (ptfe) and aisi 304 stainless steel surfaces was investigated. | 2001 | 11722659 |
| behaviour of listeria monocytogenes in packaged fresh mushrooms (agaricus bisporus). | the aim of this study was to evaluate the potential of listeria monocytogenes to grow in mushrooms packaged in two different types of pvc films when stored at 4 degrees c and 10 degrees c. | 2001 | 11722656 |
| an antibody-immobilized capillary column as a bioseparator/bioreactor for detection of escherichia coil o157:h7 with absorbance measurement. | a capillary-column-based bioseparator/bioreactor was developed for detection of escherichia coli o157:h7 by chemically immobilizing anti-e. coli o157:h7 antibodies onto the inner wall of the column, forming the "sandwich" immunocomplexes (immobilized antibody-e. coli o157: h7-enzyme-labeled antibody) after the sample and the enzyme-labeled antibody passed through the column and detecting the absorbance of the product in the bioreactor with an optical detector. the effects of the blocking agent, ... | 2001 | 11721916 |
| priming of memory but not effector cd8 t cells by a killed bacterial vaccine. | killed or inactivated vaccines targeting intracellular bacterial and protozoal pathogens are notoriously ineffective at generating protective immunity. for example, vaccination with heat-killed listeria monocytogenes (hklm) is not protective, although infection with live l. monocytogenes induces long-lived, cd8 t cell-mediated immunity. we demonstrate that hklm immunization primes memory cd8 t lymphocyte populations that, although substantial in size, are ineffective at providing protection from ... | 2001 | 11721060 |
| effects of intermediate filaments on actin-based motility of listeria monocytogenes. | how does subcellular architecture influence the intracellular movements of large organelles and macromolecular assemblies? to investigate the effects of mechanical changes in cytoplasmic structure on intracellular motility, we have characterized the actin-based motility of the intracellular bacterial pathogen listeria monocytogenes in normal mouse fibroblasts and in fibroblasts lacking intermediate filaments. the apparent diffusion coefficient of l. monocytogenes was two-fold greater in vimentin ... | 2001 | 11720985 |
| uptake and killing of listeria monocytogenes by normal human peripheral blood granulocytes and monocytes as measured by flow cytometry and cell sorting. | cellular components of innate immunity (nk cells, monocytes and granulocytes) play an important role in early resistance to listeria monocytogenes in the mouse model. minimally invasive methods of measuring the bacteriocidal capacity of these cells may be useful as a biomarker of susceptibility in humans. a technique was developed whereby the uptake and survival of l. monocytogenes could be measured in human granulocytes and monocytes using small volumes of peripheral blood. this method used flo ... | 2001 | 11720818 |
| cloning and characterization of m.lmoa118i, a novel dna:m4c methyltransferase from the listeria monocytogenes phage a118, a close homolog of m.ngomxv. | a homolog of m.ngomxv dna:m4c methyltransferase has been identified among the open reading frames deduced from the genomic sequence of listeria monocytogenes phage a118 [loessner et al., 2000]. the gene coding for this putative protein has been cloned in escherichia coli and its enzymatic activity in vivo in this host have been analyzed. remarkably, despite m.ngomxv and m.lmoa118i exhibit high sequence similarity (58% identical and 19% conservatively substituted residues), their target preferenc ... | 2001 | 11720310 |
| detection of listeria monocytogenes by polymerase chain reaction oriented to inlb gene. | primers were designed for the detection of listeria monocytogenes by the polymerase chain reaction oriented to specific sequences of the inlb gene encoding an internalin. at optimized reaction conditions, 100% sensitivity (on a panel of 33 strains of l. monocytogenes) and 100% specificity (on panels of 15 strains of other listeria spp. and 41 other bacteria), were determined for the inlb-l/r primers. the detection limit of pcr with these primers was 10(4) cfu/ml and was not affected by up to 10( ... | 2001 | 11718370 |
| prevalence and characterization of listeria monocytogenes in the feces of healthy austrians. | the aims of the study were to determine the prevalence of listeria monocytogenes in the feces of healthy austrians and to characterize the isolates by various typing methods. stool specimens from 505 healthy volunteers from the tyrol were tested for the presence of l. monocytogenes using cold enrichment for 6 months and five different detection methods: conventional plating onto palcam and rapid'l.mono agar, immunomagnetic separation (ims) followed by conventional plating, enzyme-linked fluoresc ... | 2001 | 11715752 |
| immunization with gp96 from listeria monocytogenes-infected mice is due to n-formylated listerial peptides. | n-formylated (n-f-met) peptides derived from proteins of the intracellular bacterium listeria monocytogenes generate a protective, h2-m3-restricted cd8 t cell response in c57bl/6 mice. n-f-met peptide-specific ctl were generated in vitro when mice previously immunized with gp96 isolated from donor mice infected with l. monocytogenes were stimulated with these peptides. no significant peptide-specific ctl activity was observed in mice immunized with gp96 from uninfected animals. masses correspond ... | 2001 | 11714815 |
| two listeria monocytogenes vaccine vectors that express different molecular forms of human papilloma virus-16 (hpv-16) e7 induce qualitatively different t cell immunity that correlates with their ability to induce regression of established tumors immortalized by hpv-16. | two recombinant listeria monocytogenes (rlm) strains were produced that secrete the human papilloma virus-16 (hpv-16) e7 protein expressed in hpv-16-associated cervical cancer cells. one, lm-e7, expresses and secretes e7 protein, whereas a second, lm-llo-e7, secretes e7 as a fusion protein joined to a nonhemolytic listeriolysin o (llo). lm-llo-e7, but not lm-e7, induces the regression of the e7-expressing tumor, tc-1, established in syngeneic c57bl/6 mice. both recombinant e7-expressing rlm vacc ... | 2001 | 11714814 |
| tolerance or immunity to a tumor antigen expressed in somatic cells can be determined by systemic proinflammatory signals at the time of first antigen exposure. | mice transgenic for the e7 tumor ag of human papillomavirus type 16, driven from a keratin 14 promoter, express e7 in keratinocytes but not dendritic cells. grafted e7-transgenic skin is not rejected by e7-immunized mice that reject e7-transduced transplantable tumors. rejection of recently transplanted e7-transgenic skin grafts, but not of control nontransgenic grafts or of established e7-transgenic grafts, is induced by systemic administration of live or killed listeria monocytogenes or of end ... | 2001 | 11714778 |
| effect of 6-hydroxydopamine on host resistance against listeria monocytogenes infection. | recent studies have shown that immunocompetent cells bear receptors of neuropeptides and neurotransmitters and that these ligands play roles in the immune response. in this study, the role of the sympathetic nervous system in host resistance against listeria monocytogenes infection was investigated in mice pretreated with 6-hydroxydopamine (6-ohda), which destroys sympathetic nerve termini. the norepinephrine contents of the plasma and spleens were significantly lower in 6-ohda-treated mice than ... | 2001 | 11705892 |
| exaggerated proinflammatory and th1 responses in the absence of gamma/delta t cells after infection with listeria monocytogenes. | while gamma/delta t cells are involved in host defense and immunopathology in a variety of infectious diseases, their precise role is not yet clearly defined. in the absence of gamma/delta t cells, mice die after infection with a dose of listeria monocytogenes that is not lethal in immunologically intact animals. morbidity might result from insufficient levels of cytokines normally produced by gamma/delta t cells or conversely from an excess of cytokines due to a lack of down-regulation of the i ... | 2001 | 11705890 |
| intestinal infection of balb/c mice with yersinia enterocolitica o9 causes major modifications in phenotype and functions of spleen cells. | yersinia enterocolitica serotype o9 may cause a persistent intestinal infection with few or no symptoms in humans and in balb/c mice. the present study demonstrated profound alterations in the immune status of balb/c mice infected with y. enterocolitica o9. infected mice developed splenomegaly and phenotypic analysis of spleen cells revealed increases in cd3+ total t cells, cd4+ helper t cells, cd8+ cytotoxic t cells and cd11b+ phagocytic cells. spleen cells from infected mice exhibited impaired ... | 2001 | 11700368 |
| svpa, a novel surface virulence-associated protein required for intracellular survival of listeria monocytogenes. | a previously unknown protein, designated svpa (surface virulence-associated protein) and implicated in the virulence of the intracellular pathogen listeria monocytogenes, was identified. this 64 kda protein, encoded by svpa, is both secreted in culture supernatants and surface-exposed, as shown by immunogold labelling of whole bacteria with an anti-svpa antibody. analysis of the peptide sequence revealed that svpa contains a leader peptide, a predicted c-terminal transmembrane region and a posit ... | 2001 | 11700342 |
| two consecutive nationwide outbreaks of listeriosis in france, october 1999-february 2000. | in france, listeriosis surveillance is based on mandatory notification of all culture-confirmed cases, with systematic typing of isolates and routine collection of the patient's food history. from october 1999 to march 2000, two outbreaks of listeriosis were detected through this enhanced surveillance system. in outbreak 1, analysis of the food histories of cases suggested brand x "rillettes," a pâté-like meat product, as the vehicle of infection, and the outbreak strain of listeria monocytogene ... | 2001 | 11700249 |
| prevalence of foodborne pathogens in bulk tank milk. | bulk tank milk from 131 dairy herds in eastern south dakota and western minnesota was examined for the presence of for foodborne pathogens. campylobacter jejuni, shiga-toxin producing escherichia coli, listeria monocytogenes, salmonella spp., and yersinia enterocolitica were detected in 9.2, 3.8, 4.6, 6.1, and 6.1% of bulk tank milk samples, respectively. thirty-five of 131 (26.7%) bulk tank milk samples contained one or more species of pathogenic bacteria. isolates of salmonella belonged to gro ... | 2001 | 11699446 |
| role of cd28 for the generation and expansion of antigen-specific cd8(+) t lymphocytes during infection with listeria monocytogenes. | infection of mice with the intracellular bacterium listeria monocytogenes results in a strong cd8(+) t cell response that is critical for efficient control of infection. we used cd28-deficient mice to characterize the function of cd28 during listeria infection, with a main emphasis on listeria-specific cd8(+) t cells. frequencies and effector functions of these t cells were determined using mhc class i tetramers, single cell ifn-gamma production and listeria-specific cytotoxicity. during primary ... | 2001 | 11698433 |
| listeria monocytogenes infection overcomes the requirement for cd40 ligand in exogenous antigen presentation to cd8(+) t cells. | in vivo priming of cd8(+) t lymphocytes against exogenously processed model ags requires cd4(+) t cell help, specifically interactions between cd40 ligand (cd40l) expressed by activated cd4(+) t cells and cd40, which is present on professional apc such as dendritic cells (dcs). to address this issue in the context of bacterial infection, we examined cd40l-cd40 interactions in cd8(+) t cell priming against an exogenously processed, nonsecreted bacterial ag. cd40l interactions were blocked by in v ... | 2001 | 11698431 |
| evaluation of a spray-dried lacticin 3147 powder for the control of listeria monocytogenes and bacillus cereus in a range of food systems. | the potential of a powdered preparation of the bacteriocin, lacticin 3147, was investigated for the inhibition of listeria monocytogenes and bacillus cereus. | 2001 | 11696102 |
| the effect of inoculum size and sublethal injury on the ability of listeria monocytogenes to initiate growth under suboptimal conditions. | to investigate the effect of inoculum size and physiological state on the ability of listeria monocytogenes cells to initiate growth under suboptimal conditions of salt concentration and ph. | 2001 | 11696096 |
| bactericidal properties of human and murine groups i, ii, v, x, and xii secreted phospholipases a(2). | group iia secreted phospholipase a(2) (spla2) is known to display potent gram-positive bactericidal activity in vitro and in vivo. we have analyzed the bactericidal activity of the full set of recombinant murine and human groups i, ii, v, x, and xii spla2s on listeria monocytogenes, staphylococcus aureus, and escherichia coli. the rank order potency among human spla2s against gram-positive bacteria is group iia > x > v > xii > iie > ib, iif (for murine spla2s: iia > iid > v > iie > iic, x > ib, ... | 2002 | 11694541 |
| evaluation of caesalpinia ferrea extract on bone marrow hematopoiesis in the murine models of listeriosis and ehrlich ascites tumor. | the capacity of hematopoietic tissues to produce and mobilize phagocytes to the site of infection and tumor growth is of central importance to mediate the early immunological response. in this perspective, studies from our laboratory have defined listeria monocytogenes infection and the ehrlich ascites tumor (eat) as useful models to investigate the effects of natural compounds on the growth and differentiation of granulocyte-macrophage progenitor cells (cfu-gm). as expected, a significant reduc ... | 2001 | 11694028 |
| polymerase chain reaction for detection of listeria monocytogenes. | 2002 | 11692870 | |
| [spontaneous bacterial peritonitis caused by listeria monocytogenes in a patient with liver cirrhosis]. | 2001 | 11692785 | |
| rapidly growing tumor-like brain lesion. | listeria monocytogenes accounts for 8-11% of the cases of bacterial meningitis which is associated with high mortality in patients with serious underlying diseases or those receiving immunosuppressive treatment. brain abscess due to l. monocytogenes is a very rare occurrence. the case reported here concerns a 54-year-old female patient with a rapidly growing tumor-like brain lesion. l. monocytogenes type 4b could be cultured from blood and brain biopsy. despite antimicrobial therapy with ampicil ... | 2001 | 11688907 |
| rapd analysis, serotyping, and esterase typing indicate that the population of listeria monocytogenes strains recovered from cheese and from patients with listeriosis in belgium are different. | populations of listeria monocytogenes strains isolated in belgium from cheese and from patients with listeriosis were characterised by randomly amplified polymorphic dna (rapd) analysis using two 10-mers primers (opa-04 and opa-13). high discrimination levels were obtained with each of these primers alone (discrimination indices (di) of 0.899 and 0.935 for opa13 and opa04, respectively) or in combination (di of 0.960). the clustering of strains obtained by rapd was compared with a clustering pre ... | 2001 | 11683471 |
| listeria monocytogenes response regulators important for stress tolerance and pathogenesis. | environmental sensing by two-component signal transduction systems is likely to play a role for growth and survival of listeria monocytogenes both during transmission in food products and within a host organism. two-component systems typically consist of a membrane-associated sensor histidine kinase and a gene regulatory protein, the response regulator (rr). we have identified seven putative rr genes in l. monocytogenes lo28 by pcr using degenerate oligonucleotide primers. by insertional inactiv ... | 2001 | 11682188 |
| comparative genomics of listeria species. | listeria monocytogenes is a food-borne pathogen with a high mortality rate that has also emerged as a paradigm for intracellular parasitism. we present and compare the genome sequences of l. monocytogenes (2,944,528 base pairs) and a nonpathogenic species, l. innocua (3,011,209 base pairs). we found a large number of predicted genes encoding surface and secreted proteins, transporters, and transcriptional regulators, consistent with the ability of both species to adapt to diverse environments. t ... | 2001 | 11679669 |
| pyrosequencing as a method for grouping of listeria monocytogenes strains on the basis of single-nucleotide polymorphisms in the inlb gene. | by using pyrosequencing (i.e., sequencing by synthesis) 106 strains of different serovars of listeria monocytogenes were rapidly grouped into four categories based on nucleotide variations at positions 1575 and 1578 of the inlb gene. strains of serovars 1/2a and 1/2c constituted one group, and strains of serovars 1/2b and 3b constituted another group, whereas serovar 4b strains were separated into two groups. | 2001 | 11679367 |
| in vitro transcription of prfa-dependent and -independent genes of listeria monocytogenes. | in vitro transcription starting from the promoters of the listeria monocytogenes genes hly, plca, acta, mpl, prfa and iap has been studied. whereas transcription from phly, pplca and pacta is strictly prfa-dependent, that from piap, pprfa1/2 and, unexpectedly, also from pmpl is independent. initiation of in vitro transcription at all tested promoters except pprfa requires high concentrations of atp but not gtp. the nucleotides required in higher concentrations for efficient in vitro transcriptio ... | 2001 | 11679071 |
| virologic and immunologic parameters that predict clinical response of aids-associated kaposi's sarcoma to highly active antiretroviral therapy. | the purpose of the work was to assess the predictive value of biologic factors on the efficacy of highly active antiretroviral therapy alone or combined with chemotherapy on aids-associated kaposi's sarcoma. twenty-six aids-kaposi's sarcoma patients who started therapy with protease inhibitors were investigated. no baseline chemotherapy was associated with less severe initial clinical status. median follow-up was 652 d. the main outcome measures were as follows: best kaposi's sarcoma clinical re ... | 2001 | 11676823 |
| fulminant hepatitis b virus reactivation with concomitant listeriosis after fludarabine and rituximab therapy: case report. | chronic hepatitis b infection poses a problem in patients with malignancies undergoing chemotherapy. not uncommonly, hepatitis b virus (hbv) reactivates during or soon after chemotherapy. we report a case of a woman with acute prolymphocytic leukemia who received chemotherapy containing fludarabine and cyclophosphamide, followed by the cd 20 monoclonal antibody, rituximab. she developed fatal reactivation of hepatitis b with fulminant liver failure 3 months after completing treatment. listeria m ... | 2001 | 11669307 |
| epidural abscess and vertebral osteomyelitis caused by listeria monocytogenes: case report and literature review. | a 69-y-old male with an epidural abscess and concomitant vertebral osteomyelitis caused by listeria monocytogenes is presented, together with a brief review of the literature. to our knowledge, no cases of epidural abscess and only 3 cases of osteomyelitis have previously been reported in association with this organism. | 2001 | 11669234 |
| effects of vegetable type, package atmosphere and storage temperature on growth and survival of escherichia coli o157:h7 and listeria monocytogenes. | the survival and growth of escherichia coli o157:h7 (atcc 43888 and nctc 12900) and listeria monocytogenes (atcc 19114 and nctc 11994) during storage (4 and 8 degrees c) on ready-to-use (rtu) packaged vegetables (lettuce, swedes (rutabaga), dry coleslaw mix, soybean sprouts) were studied. the vegetables were sealed within oriented polypropylene packaging film, and modified atmospheres developed in packs during storage due to produce respiration. survival and growth patterns were dependent on veg ... | 2001 | 11641769 |
| transfer of eukaryotic expression plasmids to mammalian host cells by bacterial carriers. | the concept of transkingom transfer of dna from bacteria to other organisms has recently been extended to include eukaryotic host cells. intracellular bacteria have been shown to transfer eukaryotic expression plasmids to mammalian host cells in vitro and in vivo. this can be used to induce immune responses towards protein antigens encoded by the plasmid, to complement genetic defects or even to direct the production of proteins in appropriate organs. the ease of generating such vehicles makes t ... | 2001 | 11604322 |
| random amplification of polymorphic dna typing of listeria monocytogenes isolated from meat. | to investigate the epidemiological characteristics of listeria monocytogenes isolated from imported or domestic meats, l. monocytogenes was isolated and identified through biochemical and serological tests, and epidemiological analysis of the isolates was carried out through the random amplification of polymorphic dna (rapd) method. fifty-four isolates were identified as l. monocytogenes through biochemical tests, of which 36 (67%) were confirmed as serotype 1, and 18 (33%) were serotype 4, thro ... | 2001 | 11603860 |
| inhibitory activity of honey against foodborne pathogens as influenced by the presence of hydrogen peroxide and level of antioxidant power. | antimicrobial activity of honey has been attributed to hydrogen peroxide, which is produced by naturally occurring glucose oxidase, and phenolic compounds, although lethality of and inhibition by these and other components against microorganisms vary greatly, depending on the floral source of nectar. this study was undertaken to compare honeys from six floral sources for their inhibitory activity against escherichia coli o157:h7, salmonella typhimurium, shigella sonnei, listeria monocytogenes, s ... | 2001 | 11603859 |
| [emerging new risks of food-borne infections]. | 2001 | 11602389 | |
| a possible regulatory role for the metal-binding domain of cada, the listeria monocytogenes cd2+-atpase. | using the baculovirus/sf9 expression system, we produced cada and deltambd, a metal-binding domain, truncated cada. both proteins had the expected properties of p-type atpases: atp-induced cd2+ accumulation, cd2+-sensitive atp and pi phosphorylation and atpase activity. deltambd displayed lower initial transport velocity as well as lower maximal atpase activity than cada. mbd truncation flattened the cd2+ dependence of the atpase activity and increased apparent cd2+ affinity, suggesting a positi ... | 2001 | 11602255 |
| efficacy of trisodium phosphate solutions in reducing listeria monocytogenes populations on chicken skin during refrigerated storage. | chicken skin inoculated with l0(8) cfu/ml of listeria monocytogenes was dipped for 15 min in sterile water (control) and in 8, 10, or 12% trisodium phosphate (tsp) solutions. skin samples were stored at 2 degrees c for 5 days, with microbial monitoring on days 0, 1, 3, and 5 after treatment. compared to the water dip, all tsp treatments significantly (p < 0.05) reduced l monocytogenes populations on chicken skin. the concentration of the tsp was a significant factor in reducing the populations o ... | 2001 | 11601719 |
| efficacy of chitosan, carvacrol, and a hydrogen peroxide-based biocide against foodborne microorganisms in suspension and adhered to stainless steel. | the ability of natural compounds to inactivate foodborne organisms adhered to surfaces was investigated with the ultimate aim of replacing synthetic biocides by more environmentally friendly, natural alternatives. the antimicrobial efficacy of 0.5, 1.0, and 2.0% chitosan and spor-klenz rtu (a commercial biocide based on hydrogen peroxide and peroxyacetic acid) and 0.5, 1.25, and 2.0 mm carvacrol was determined at 20 degrees c against listeria monocytogenes, salmonella enterica serovar typhimuriu ... | 2001 | 11601703 |
| comparative evaluation of culture- and bax polymerase chain reaction-based detection methods for listeria spp. and listeria monocytogenes in environmental and raw fish samples. | two commercial polymerase chain reaction (pcr)-based listeria detection systems, the bax for screening/listeria monocytogenes and the bax for screening/genus listeria, and a culture-based detection system, the biosynth l. monocytogenes detection system (lmds), were evaluated for their ability to detect l. monocytogenes and listeria spp. in raw ingredients and the processing environment. for detection of l. monocytogenes from raw fish, enrichment was performed in listeria enrichment broth (leb), ... | 2001 | 11601700 |
| effect of sampling method on the representative recovery of microorganisms from the surfaces of aquacultured finfish. | the objective of this study was to determine if a gentle rinse procedure was equivalent to the combination of excision and homogenization with a stomacher for the relative removal of various microorganisms from finfish fillets. fillets of hybrid striped bass and rainbow trout were obtained from local markets and sampled using three methods: rinse (r), excision followed by homogenization in a stomacher (s), and homogenization of fillets following a rinse (rs). microorganisms were enumerated on se ... | 2001 | 11601699 |
| [listeria monocytogenes aortitis]. | 2001 | 11599169 | |
| administration of superantigens protects mice from lethal listeria monocytogenes infection by enhancing cytotoxic t cells. | superantigens stimulate t-cell-receptor vbeta-selective t-cell proliferation accompanying the release of cytokines, which may eventually protect the host from microbial infections. we investigated here whether superantigens can rescue the host from lethal bacterial infection. mice were pretreated with staphylococcus aureus enterotoxin b (seb) 1 and 2 days before bacterial infection, and the mortality of infected mice was assessed. seb pretreatment protected mice from lethal infection with lister ... | 2001 | 11598032 |
| the family of thiol-activated, cholesterol-binding cytolysins. | several species of both pathogenic and non-pathogenic grampositive bacteria within the genera streptococcus, clostridium and bacillus secrete cytolytic proteins that belong to a single, highly homologous family. the most widely known members of this family are streptolysin o, listeriolysin, perfringolysin, and pneumolysin. these toxins specifically require membrane cholesterol but, apparently, do not depend on any other specific cell surface receptor, so that they are able to lyse the cytoplasmi ... | 2001 | 11595631 |
| quantifying the relationship between multiple immunological parameters and host resistance: probing the limits of reductionism. | although reductionist experimental designs are excellent for identifying cells, molecules, or functions involved in resistance to particular microbes or cancer cells, they do not provide an integrated, quantitative view of immune function. in the present study, mice were treated with either dexamethasone (dex) or cyclosporin a (cya), and immune function and host resistance were evaluated. multivariate statistical methods were used to describe the relative importance of a broad range of immunolog ... | 2001 | 11591782 |
| intraoperative prostaglandin e1 improves antimicrobial and inflammatory responses in alveolar immune cells. | anesthesia and surgery decrease antimicrobial and increase proinflammatory functions of alveolar immune cells. thus, anti-inflammatory agents that do not further suppress antimicrobial functions are required. we tested the hypothesis that intraoperative prostaglandin e1 (pge1) suppresses proinflammatory responses and prevents the reduction in antimicrobial responses of alveolar immune cells. | 2001 | 11588457 |
| inhibition of interleukin-12 production by auranofin, an anti-rheumatic gold compound, deviates cd4(+) t cells from the th1 to the th2 pathway. | 1. interleukin-12 (il-12) may play a central role in the development and progression of rheumatoid arthritis by driving the immune response towards t helper 1 (th1) type responses characterized by high ifn-gamma and low il-4 production. in this study we investigated the effect of auranofin (af), an anti-rheumatic gold compound, on il-12 production in mouse macrophages and dendritic cells, and studied whether af-mediated inhibition of il-12 production could regulate a cytokine profile of antigen ... | 2001 | 11588111 |
| detection of low levels of listeria monocytogenes within 20 hours using an evanescent wave biosensor. | 2001 | 11586938 | |
| difference in cholesterol-binding and cytolytic activities between listeriolysin o and seeligeriolysin o: a possible role of alanine residue in tryptophan-rich undecapeptide. | we have constructed recombinant listeriolysin o (rllo) and seeligeriolysin o (rlso) from listeria monocytogenes and listeria seeligeri, respectively. in hemolysis and cholesterol-binding assays, the specific activity of recombinant toxin was lower for lso as compared to llo. to understand the molecular basis of this difference, in particular with respect to the conserved trp-rich undecapeptide, a naturally occurring ala to phe substitution in lso was introduced into rllo. the rllo:a488f hemolysi ... | 2001 | 11583846 |
| pivotal role of vasp in arp2/3 complex-mediated actin nucleation, actin branch-formation, and listeria monocytogenes motility. | the listeria monocytogenes acta protein mediates actin-based motility by recruiting and stimulating the arp2/3 complex. in vitro, the actin monomer-binding region of acta is critical for stimulating arp2/3-dependent actin nucleation; however, this region is dispensable for actin-based motility in cells. here, we provide genetic and biochemical evidence that vasodilator-stimulated phosphoprotein (vasp) recruitment by acta can bypass defects in actin monomer-binding. furthermore, purified vasp enh ... | 2001 | 11581288 |