Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| enteropathogenic bacteria in faecal swabs of young children fed on lactic acid-fermented cereal gruels. | the influence of consumption of a lactic acid-fermented cereal gruel togwa with ph < or = 4 on the presence of faecal enteric bacteria such as campylobacter, enterohaemorrhagic escherichia coli (ehec:o157), enterotoxigenic escherichia coli (etec), salmonella and shigella was evaluated. under 5 years old healthy children listed in an ascending order of age were alternatively assigned and given either a lactic-acid fermented cereal gruel togwa (test diet) or an unfermented cereal gruel uji (contro ... | 1999 | 10098782 |
| the risk of guillain-barré syndrome following infection with campylobacter jejuni. | to estimate the incidence of guillain-barré syndrome (gbs) following campylobacter jejuni infection (ci) we studied three populations where outbreaks of ci had occurred involving an estimated 8000 cases. no case of gbs was detected in the 6 months following the outbreaks in the local populations. the point estimate for the risk of gbs following ci estimated in this study was 0 in 8000 (95% confidence interval 0-3). | 1999 | 10098780 |
| mutational analysis of the vaca promoter provides insight into gene transcription in helicobacter pylori. | analysis of 12 helicobacter pylori promoters indicates the existence of a consensus -10 hexamer (tataat) but little conservation of -35 sequences. in this study, mutations in either the h. pylori vaca -10 region or the -35 region resulted in decreased vaca transcription and suggested that an extended -10 motif is utilized. thus, despite the lack of a -35 consensus sequence for h. pylori promoters, the -35 region plays a functional role in vaca transcription. | 1999 | 10094707 |
| the bspa locus of lactobacillus fermentum br11 encodes an l-cystine uptake system. | bspa is a basic surface-exposed protein from lactobacillus fermentum br11. sequence comparisons have shown that it is a member of family iii of the solute binding proteins. it is 89% identical to the collagen binding protein, cnb, from lactobacillus reuteri. compared with the database of escherichia coli proteins, bspa is most similar to the l-cystine binding protein fliy. to investigate the function of bspa, mutants depleted for bspa were generated by homologous recombination with a temperature ... | 1999 | 10094698 |
| spectrum and antimicrobial activity of alexomycin (pnu-82, 127), a peptide compound projected for use in animal health. | alexomycin (pnu-82, 127) is a thiopeptide antimicrobial complex intended for veterinary practice that belongs to a series of cyclic peptides produced by streptomyces arginensis. mics against selected routine and fastidious clinical isolates of animal and human origin were determined by broth microdilution or agar dilution reference methods. alexomycin was active against gram-positive pathogens such as oxacillin-susceptible and -resistant staphylococcus aureus and coagulase-negative staphylococci ... | 1999 | 10092967 |
| expression, purification, crystallization and preliminary x-ray diffraction results from campylobacter jejuni ferritin. | the prokaryotic ferritin gene of campylobacter jejuni was overexpressed in escherichia coli under control of the bacteriophage t7 promoter and the protein (cj-ftn) purified. preliminary crystallization experiments have been performed using the hanging-drop vapour-diffusion method with ammonium sulfate as the precipitant. diffraction studies show the crystals belong to the i432 space group (a = 151.52 a). structure solution by molecular replacement is in progress while crystal quality improvement ... | 1999 | 10089431 |
| [lumbar puncture in a pediatric emergency department: something more than a diagnostic technic]. | the aim of this study was to know the incidence of serious bacterial infections (sbi) in children without sepsis or intracranial infection in which spinal puncture (lp) was performed in an emergency department. | 1999 | 10083638 |
| campylobacter jejuni--an emerging foodborne pathogen. | campylobacter jejuni is the most commonly reported bacterial cause of foodborne infection in the united states. adding to the human and economic costs are chronic sequelae associated with c. jejuni infection--guillian-barré syndrome and reactive arthritis. in addition, an increasing proportion of human infections caused by c. jejuni are resistant to antimicrobial therapy. mishandling of raw poultry and consumption of undercooked poultry are the major risk factors for human campylobacteriosis. ef ... | 1999 | 10081669 |
| [campylobacter fetus bacteremia and thrombophlebitis in a patient with waldenstrom's macroglobulinemia]. | we report a 67-year-old male with waldenstrom's macroglobulinemia who developed campylobacter fetus subspesis fetus (c. fetus) bacteremia and thrombophlebitis. the patient developed a fever and pain in his left lower limb, and could not walk because of the pain. radioisotopic venography showed thrombophlebitis in his left lower limb. his blood culture grew c. fetus. after starting intravenous papm/bp, his symptoms resolved promptly. in contrast to campylobacter jejuni which is a common cause of ... | 1999 | 10077907 |
| the use of gene clusters to infer functional coupling. | previously, we presented evidence that it is possible to predict functional coupling between genes based on conservation of gene clusters between genomes. with the rapid increase in the availability of prokaryotic sequence data, it has become possible to verify and apply the technique. in this paper, we extend our characterization of the parameters that determine the utility of the approach, and we generalize the approach in a way that supports detection of common classes of functionally coupled ... | 1999 | 10077608 |
| cloning, mutation and distribution of a putative lipopolysaccharide biosynthesis locus in campylobacter jejuni. | a region encoding orfs with homology to known lipopolysaccharide (lps) biosynthesis genes was isolated from two strains of campylobacter jejuni. one of the strains produces lps, but the second strain is reported to produce only lipooligosaccharide (los) and therefore lacks the o-chain. the two strains shared six predicted orfs, but an additional orf, orfe, of unknown function was identified in the los-producing strain. mutation of the shared wbee (rfbe) homologue (orff) or deletion of five of th ... | 1999 | 10075420 |
| identification and characterization of is2404 and is2606: two distinct repeated sequences for detection of mycobacterium ulcerans by pcr. | molecular analysis of mycobacterium ulcerans has revealed two new insertion sequences (iss), is2404 and is2606. is2404 was identified by complete sequencing of a previously described repetitive dna segment from m. ulcerans. this element is 1,274 bp long, contains 12-bp inverted repeats and a single open reading frame (orf) potentially encoding a protein of 327 amino acids (aa), and apparently generates 7-bp direct repeats upon transposition. amino acid similarity was found between the putative t ... | 1999 | 10074520 |
| evaluation of accuracy and repeatability of identification of food-borne pathogens by automated bacterial identification systems. | the performances of five automated microbial identification systems, relative to that of a reference identification system, for their ability to accurately and repeatedly identify six common food-borne pathogens were assessed. the systems assessed were the microlog system (biolog inc., hayward, calif.), the microbial identification system (mis; midi inc., newark, del.), the vitek system (biomérieux vitek, hazelwood, mo.), the microscan walkaway 40 system (dade-microscan international, west sacra ... | 1999 | 10074506 |
| apoptotic effect of outer-membrane proteins from campylobacter jejuni on chicken lymphocytes. | campylobacter jejuni is a significant cause of food-borne diseases in humans. the bacterium is considered a commensal organism in chickens, and it can heavily colonize chickens without causing inflammation. poultry may be the major reservoir for the human infection in developed countries. here we show that an outer-membrane protein extract prepared from the bacteria caused apoptosis of chicken lymphocytes detected in vitro with the terminal deoxynucleotidyl transferase-mediated dutp nick end-lab ... | 1999 | 10069862 |
| changes in the evolution of the antigenic profiles and morphology during coccoid conversion of helicobacter pylori. | the significance of the coccoid forms of h. pylori is still controversial and the questions of whether these forms are viable and infective or degenerative are still open. we induced conversion from rod to coccoid forms and studied morphological changes and antigenic evolutions during this conversion and, thereby, elucidated the viability of coccoid forms. | 1999 | 10063308 |
| in situ analysis of phototrophic sulfur bacteria in the chemocline of meromictic lake cadagno (switzerland). | comparative sequence analysis of a 16s rrna gene clone library from the chemocline of the meromictic lake cadagno (switzerland) revealed the presence of a diverse number of phototrophic sulfur bacteria. sequences resembled those of rrna of type strains chromatium okenii dsm169 and amoebobacter purpureus dsm4197, as well as those of four bacteria forming a tight cluster with a. purpureus dsm4197 and lamprocystis roseopersicina dsm229. in situ hybridization with fluorescent (cy3 labeled) oligonucl ... | 1999 | 10049902 |
| a randomly amplified polymorphic dna marker specific for the bacillus cereus group is diagnostic for bacillus anthracis. | aiming to develop a dna marker specific for bacillus anthracis and able to discriminate this species from bacillus cereus, bacillus thuringiensis, and bacillus mycoides, we applied the randomly amplified polymorphic dna (rapd) fingerprinting technique to a collection of 101 strains of the genus bacillus, including 61 strains of the b. cereus group. an 838-bp rapd marker (sg-850) specific for b. cereus, b. thuringiensis, b. anthracis, and b. mycoides was identified. this fragment included a putat ... | 1999 | 10049896 |
| comparison of flagellin genes from clinical and environmental pseudomonas aeruginosa isolates. | pseudomonas aeruginosa, an important opportunistic pathogen, was isolated from environmental samples and compared to clinically derived strains. while p. aeruginosa was isolated readily from an experimental mushroom-growing unit, it was found only rarely in other environmental samples. a flagellin gene pcr-restriction fragment length polymorphism analysis of the isolates revealed that environmental and clinical p. aeruginosa strains are not readily distinguishable. the variation in the central r ... | 1999 | 10049879 |
| physiological characterization of viable-but-nonculturable campylobacter jejuni cells. | campylobacter jejuni is a pathogenic, microaerophilic, gram-negative, mesophilic bacterium. three strains isolated from humans with enteric campylobacteriosis were able to survive at high population levels (10(7) cells ml-1) as viable-but-nonculturable (vbnc) forms in microcosm water. the vbnc forms of the three c. jejuni strains were enumerated and characterized by using 5-cyano-2,3-ditolyl tetrazolium chloride-4',6-diamino-2-phenylindole staining. cellular volume, adenylate energy charge, inte ... | 1999 | 10049870 |
| ferritin mutants of escherichia coli are iron deficient and growth impaired, and fur mutants are iron deficient. | escherichia coli contains at least two iron storage proteins, a ferritin (ftna) and a bacterioferritin (bfr). to investigate their specific functions, the corresponding genes (ftna and bfr) were inactivated by replacing the chromosomal ftna and bfr genes with disrupted derivatives containing antibiotic resistance cassettes in place of internal segments of the corresponding coding regions. single mutants (ftna::spc and bfr::kan) and a double mutant (ftna::spc bfr::kan) were generated and confirme ... | 1999 | 10049371 |
| lymphocytic colitis: a clue to an infectious trigger. | we present a 19-year-old patient who was admitted for evaluation of prolonged watery diarrhea. previous study showed one stool culture positive for campylobacter jejuni, which was treated with appropriate antibiotics with no response. she underwent colonoscopy with multiple biopsies, which led to a diagnosis of lymphocytic colitis. we believe that the patient's disease was due to the infectious process, which triggered an autoimmune response and caused the lymphocytic colitis. | 1999 | 10048742 |
| etiology of outpatient pediatric nondysenteric diarrhea: a multicenter study in the united states. | few data have been published recently on the etiology of outpatient pediatric diarrhea in the us. | 1999 | 10048678 |
| inducing enterotoxigenic properties in campylobacter jejuni and campylobacter coli by serial intraperitoneal passage in mice. | 1999 | 10029919 | |
| characterization of human bactericidal antibodies to bordetella pertussis. | the bordetella pertussis brka protein protects against the bactericidal activity of complement and antibody; however, some individuals mount an immune response that overcomes this bacterial defense. to further characterize this process, the bactericidal activities of sera from 13 adults with different modes of exposure to b. pertussis (infected as adults, occupational exposure, immunized with an acellular vaccine, or no identified exposure) against a wild-type strain and a brka complement-sensit ... | 1999 | 10024590 |
| identification of a cytolethal distending toxin gene locus and features of a virulence-associated region in actinobacillus actinomycetemcomitans. | a genetic locus for a cytolethal distending toxin (cdt) was identified in a polymorphic region of the chromosome of actinobacillus actinomycetemcomitans, a predominant oral pathogen. the locus was comprised of three open reading frames (orfs) that had significant amino acid sequence similarity and more than 90% sequence identity to the cdtabc genes of some pathogenic escherichia coli strains and haemophilus ducreyi, respectively. sonic extracts from recombinant e. coli, containing the a. actinom ... | 1999 | 10024565 |
| cloning and expression of the dnak gene of campylobacter jejuni and antigenicity of heat shock protein 70. | campylobacter jejuni is a leading cause of infectious diarrhea throughout the world. in addition, there is growing evidence that guillain-barré syndrome, an inflammatory demyelinating disease of the peripheral nervous system, is frequently preceded by c. jejuni infection. in the present study, the hrca-grpe-dnak gene cluster of c. jejuni was cloned and sequenced. the dnak gene consists of an open reading frame of 1,869 bp and encodes a protein with a high degree of homology to other bacterial 70 ... | 1999 | 10024560 |
| quorum sensing in escherichia coli, salmonella typhimurium, and vibrio harveyi: a new family of genes responsible for autoinducer production. | in bacteria, the regulation of gene expression in response to changes in cell density is called quorum sensing. quorum-sensing bacteria produce, release, and respond to hormone-like molecules (autoinducers) that accumulate in the external environment as the cell population grows. in the marine bacterium vibrio harveyi two parallel quorum-sensing systems exist, and each is composed of a sensor-autoinducer pair. v. harveyi reporter strains capable of detecting only autoinducer 1 (ai-1) or autoindu ... | 1999 | 9990077 |
| identification and characterisation of a cytotoxic porin-lipopolysaccharide complex from campylobacter jejuni. | a clinical isolate of campylobacter jejuni, previously found to produce a toxin active in cell culture assays, was used for identification and characterisation of a cytotoxic porin-lipopolysaccharide (lps) complex. this cytotoxic complex was isolated by high-performance liquid chromatography of crude concentrated culture supernate and deae-anion exchange chromatography. the complex had a toxic activity of 20.1 tissue culture dose50 (tcd50)/microg of protein for hep-2 cells, 7.49 tcd50/microg of ... | 1999 | 9989641 |
| body lice as tools for diagnosis and surveillance of reemerging diseases. | body lice are vectors of three bacteria which cause human disease: rickettsia prowazekii, the agent of epidemic typhus; bartonella quintana, the agent of trench fever; and borrelia recurrentis, the agent of relapsing fever. a recrudescence of body lice is being observed as the numbers of individuals living under social conditions which predispose individuals to infestation have increased. because this phenomenon may lead to the reemergence of infections transmitted by body lice, we aimed to asse ... | 1999 | 9986818 |
| rapid detection of the chlamydiaceae and other families in the order chlamydiales: three pcr tests. | few identification methods will rapidly or specifically detect all bacteria in the order chlamydiales, family chlamydiaceae. in this study, three pcr tests based on sequence data from over 48 chlamydial strains were developed for identification of these bacteria. two tests exclusively recognized the chlamydiaceae: a multiplex test targeting the ompa gene and the rrna intergenic spacer and a taqman test targeting the 23s ribosomal dna. the multiplex test was able to detect as few as 200 inclusion ... | 1999 | 9986815 |
| identification of the enteropathogens campylobacter jejuni and campylobacter coli based on the cadf virulence gene and its product. | campylobacter jejuni and campylobacter coli are common causes of gastroenteritis in humans. infection with c. jejuni or c. coli is commonly acquired by eating undercooked chicken. the goal of this study was to develop specific detection assays for c. jejuni and c. coli isolates based on the cadf virulence gene and its product. the cadf gene from c. jejuni and c. coli encodes a 37-kda outer membrane protein that promotes the binding of these pathogens to intestinal epithelial cells. a fragment of ... | 1999 | 9986804 |
| toxic megacolon associated with campylobacter jejuni colitis. | 1999 | 9934778 | |
| expression of the flgfg operon of campylobacter jejuni in escherichia coli yields an extra fusion protein. | two campylobacter jejuni genes with homology to the escherichia coli flgf and flgg genes encoding two of the basal body rod proteins were isolated, and the nucleotide sequence was determined and analyzed. these two c. jejuni genes were shown, by northern hybridization analysis, to function as a single operon (flgfg). two transcriptional start sites were detected upstream of flgf, corresponding to the two rna transcripts detected in the northern blot. western blot immunoassays using anti-flgf and ... | 1998 | 9931471 |
| human milk glycoconjugates that inhibit pathogens. | breast-fed infants have lower incidence of diarrhea, respiratory disease, and otitis media. the protection by human milk has long been attributed to the presence of secretory iga. however, human milk contains large numbers and amounts of complex carbohydrates, including glycoproteins, glycolipids, glycosaminoglycans, mucins, and especially oligosaccharides. the oligosaccharides comprise the third most abundant solid constituent of human milk, and contain a myriad of structures. complex carbohydr ... | 1999 | 9927761 |
| detection of gyra mutations among 335 pseudomonas aeruginosa strains isolated in japan and their susceptibilities to fluoroquinolones. | gyra point mutations in 335 clinical pseudomonas aeruginosa isolates were examined mainly by nonisotopic single-strand conformation polymorphism analysis and direct sequencing. seven types of missense gyra mutations were observed in 70 of 335 strains (20.9%), and ciprofloxacin mics were > or = 3.13 micrograms/ml for 63 of 70 strains (90.0%). these included two double point mutations and three novel mutations (ala-67-->ser plus asp-87-->gly, ala-84-->pro, and gln-106-->leu). thr-83-->ile mutants ... | 1999 | 9925546 |
| a putative adhesin gene cloned from campylobacter jejuni. | thirteen campylobacter jejuni strains of human origin showed differing behaviours when analysed for their ability to bind the caco-2 cell line in vitro, suggesting variations in genetic complements and/or regulation. we designed an oligonucleotide probe corresponding to a highly conserved part of adhesins from various gram-negative bacteria. among our laboratory collection, southern hybridization has demonstrated that only a discrete number of strains harbour this sequence. the corresponding gen ... | 1998 | 9921579 |
| sequence comparison of outer membrane phospholipases a: implications for structure and for the catalytic mechanism. | in this study, the nucleotide sequence of the enterobacter agglomerans plda gene encoding outer membrane phospholipase a (ompla; ec 3.1.1.32) was determined. five other ompla amino acid sequences have previously been described, and screening of data bases of whole genome sequencing projects revealed the presence of proteins with homology to ompla in helicobacter pylori, campylobacter jejuni, yersinia pestis, neisseria menigitidis and neisseria gonorrhoeae. comparison of these eleven ompla amino ... | 1998 | 9921577 |
| procedure for the investigation of bacterial genomes: random shot-gun cloning, sample sequencing and mutagenesis of campylobacter jejuni. | 1999 | 9894591 | |
| campylobacter jejuni infection and guillain-barré syndrome: a case-control study. emilia-romagna study group on clinical and epidemiological problems in neurology. | we performed a case-control study to investigate the association between campylobacter jejuni (cj) infection and guillain-barr| syndrome (gbs) or miller-fisher syndrome. we compared 60 cases with 109 hospital controls matched for age, gender, hospital and geographical location. to diagnose the cj infection, we considered the association between serologic positivity for cj and a previous diarrheal illness within 3 months of inclusion in the study. fifteen percent of cases versus 5% of hospital co ... | 1998 | 9890808 |
| iron storage in bacteria. | iron is an essential nutrient for nearly all organisms but presents problems of toxicity, poor solubility and low availability. these problems are alleviated through the use of iron-storage proteins. bacteria possess two types of iron-storage protein, the haem-containing bacterioferritins and the haem-free ferritins. these proteins are widespread in bacteria, with at least 39 examples known so far in eubacteria and archaebacteria. the bacterioferritins and ferritins are distantly related but ret ... | 1998 | 9889981 |
| gram type-specific broad-range pcr amplification for rapid detection of 62 pathogenic bacteria. | broad-range pcr has proven to be useful for the detection of bacteria. a set of broad-range pcr primers directed against conserved regions in the 16s rrna gene was designed to specifically amplify either gram-positive or gram-negative bacteria. the gram type-specific broad-range pcr correctly classified all 62 pathogenic species tested. | 1999 | 9889245 |
| pcr-enzyme-linked immunosorbent assay for detection and identification of campylobacter species: application to isolates and stool samples. | we report a pcr-enzyme-linked immunosorbent assay which identifies campylobacter species by capture hybridization of a single-stranded 16s rrna gene amplicon with species-specific probes in a microtiter plate format. specificities were confirmed for both reference and field strains, but the type strain of campylobacter coli was atypical. the assay was rapid, informative, and usable with stool-extracted dna. | 1999 | 9889235 |
| the cytolethal distending toxin from the chancroid bacterium haemophilus ducreyi induces cell-cycle arrest in the g2 phase. | the potent cytolethal distending toxin produced by haemophilus ducreyi is a putative virulence factor in the pathogenesis of chancroid. we studied its action on eukaryotic cells, with the long-term goal of understanding the pathophysiology of the disease. intoxication of cultured human epithelial-like cells, human keratinocytes, and hamster fibroblasts was irreversible, and appeared as a gradual distention of three- to fivefold the size of control cells. organized actin assemblies appeared conco ... | 1999 | 9884340 |
| motility of helicobacter pylori is coordinately regulated by the transcriptional activator flgr, an ntrc homolog. | sigma54 is the subunit of bacterial rna polymerase that transcribes from promoters with enhancer elements bound by enhancer-binding proteins. by computer searches of helicobacter pylori genomic sequences, chromosomal gene disruption, and rna analyses, we have identified sigma54-recognized promoters that regulate transcription of flagellar basal body and hook genes, as well as the enhancer-binding protein flgr (flagellum regulator), a transactivating protein of the ntrc family. we demonstrate tha ... | 1999 | 9882675 |
| use of genomics to identify bacterial undecaprenyl pyrophosphate synthetase: cloning, expression, and characterization of the essential upps gene. | the prenyltransferase undecaprenyl pyrophosphate synthetase (di-trans,poly-cis-decaprenylcistransferase; ec 2.5.1.31) was purified from the soluble fraction of escherichia coli by tsk-deae, ceramic hydroxyapatite, tsk-ether, superdex 200, and heparin-actigel chromatography. the protein was labeled with the photolabile analogue of the farnesyl pyrophosphate analogue (e, e)-[1-3h]-(2-diazo-3-trifluoropropionyloxy)geranyl diphosphate and was detected on a sodium dodecyl sulfate-polyacrylamide gel a ... | 1999 | 9882662 |
| antiseptics and disinfectants: activity, action, and resistance. | antiseptics and disinfectants are extensively used in hospitals and other health care settings for a variety of topical and hard-surface applications. a wide variety of active chemical agents (biocides) are found in these products, many of which have been used for hundreds of years, including alcohols, phenols, iodine, and chlorine. most of these active agents demonstrate broad-spectrum antimicrobial activity; however, little is known about the mode of action of these agents in comparison to ant ... | 1999 | 9880479 |
| spirochaete-like swimming mode of campylobacter jejuni in a viscous environment. | the swimming patterns of campylobacter jejuni in environments of low and high viscosity were examined by a video tracking method. in media of low viscosity, c. jejuni swam with an average velocity of 39.3 microm/s with frequent changes in direction. the velocity of c. jejuni increased in a medium at a little higher viscosity than that of a low viscosity buffer. in addition to this, c. jejuni showed a second increase of velocity in media of a high viscosity of about 40 centipoise. the swimming pa ... | 1998 | 9879971 |
| characterisation of 16 campylobacter jejuni and c. coli typing bacteriophages. | taxonomic classification of bacteriophages specific for campylobacter jejuni and c. coli has not been reported previously. a set of 16 virulent phages, distinguishable by their lytic spectra, has been used extensively for epidemiological typing of c. jejuni and c. coli at preston public health laboratory. these phages were investigated by electron microscopy, pulsed-field gel electrophoresis and restriction endonuclease analysis. all phages had icosahedral heads and long contractile tails. accor ... | 1998 | 9879954 |
| shedding and colonization of campylobacter jejuni in broilers from day-of-hatch to slaughter age. | poultry are considered to be the primary reservoirs of campylobacter jejuni for humans. campylobacter jejuni can colonize the poultry intestinal tract and its subsequent shedding can result in environmental contamination, resulting in an increased risk of infection for the rest of the flock. at present, there is no information on the daily shedding pattern of c. jejuni in broiler chickens. thus, the purpose of this study was to determine the daily shedding pattern of c. jejuni in broiler chicken ... | 1998 | 9876841 |
| survival and growth of campylobacter jejuni after artificial inoculation onto chicken skin as a function of temperature and packaging conditions. | campylobacter jejuni is one of the major causes of food poisoning in humans. c. jejuni is also widespread in food animals, and meat and meat products derived from food animals are the most common vector of bacterial transmission to humans. to determine the role of packing and storage conditions on the replication of c. jejuni on chicken, the virulent strain c. jejuni 81116 was artificially inoculated onto chicken skin pieces (1 cm2) and stored at different temperatures and under various packagin ... | 1998 | 9874337 |
| the specificity of antibody in chickens immunised to reduce intestinal colonisation with campylobacter jejuni. | poultry consumption has been identified as a major risk factor for human infection with campylobacter jejuni in developed countries. c. jejuni is present in the gastrointestinal tract of broiler chickens at the time of slaughter, and faecal contamination of carcases during processing results in significant campylobacter loads on carcases. one approach to reducing the level of carcase contamination with c. jejuni is to control campylobacter infection in broiler chickens. to this end, the study de ... | 1998 | 9874102 |
| molecular epidemiology of campylobacter jejuni in broiler flocks using randomly amplified polymorphic dna-pcr and 23s rrna-pcr and role of litter in its transmission. | poultry has long been cited as a reservoir for campylobacter spp., and litter has been implicated as a vehicle in their transmission. chicks were raised on litter removed from a broiler house positive for campylobacter jejuni. litter was removed from the house on days 0, 3, and 9 after birds were removed for slaughter. chicks were raised on these three litters under controlled conditions in flocks of 25. none of these birds yielded c. jejuni in their cecal droppings through 7 weeks. two successi ... | 1999 | 9872787 |
| acute and chronic neuropathies: new aspects of guillain-barré syndrome and chronic inflammatory demyelinating polyneuropathy, an overview and an update. | during the last 15 years new information about clinical, electrophysiological, immunological and histopathological features of acute and chronic inflammatory neuropathies have emerged. thus, the guillain-barré syndrome (gbs) is no longer considered a simple entity. subtypes of the disorder besides the typical predominant motor manifestation, are recognized, i.e. a cranial nerve variant with ophthalmoplegia, ataxia and areflexia, an immune-mediated primary motor axonal neuropathy (aman), and a mo ... | 1998 | 9872432 |
| aerobic growth and survival of campylobacter jejuni in food and stream water. | when 40 campylobacter jejuni isolates from human clinical cases, raw chicken and water were tested, 29 (72.5%) could be adapted to grow on nutrient agar under aerobic conditions. once adapted, these isolates could grow on repeated aerobic subculture. an aerobically-grown camp. jejuni isolate survived almost as well as the same isolate grown microaerophilically in sterile chicken mince at 5 degrees c, and survival of a cocktail of camp. jejuni isolates under both atmospheres was comparable at 25 ... | 1998 | 9871351 |
| campylobacter jejuni bacteremia and guillain-barré syndrome in a renal transplant recipient. | in patients who have not undergone transplantation, guillain-barré syndrome (gbs) is typically preceded by an acute infection often sustained by campylobacter jejuni. thus far, in renal transplant recipients, only eight cases of gbs have been reported. in seven patients gbs was attributed to cytomegalovirus infection and in the eighth patient to cyclosporin a neurotoxicity. we report here the case of a gbs in a renal transplant recipient following c. jejuni bacteremia. the infection quickly disa ... | 1998 | 9870273 |
| type ii topoisomerase mutations in ciprofloxacin-resistant strains of pseudomonas aeruginosa. | we determined the sequences of the quinolone resistance-determining regions of gyra, gyrb, and parc genes for 30 clinical strains of pseudomonas aeruginosa resistant to ciprofloxacin that were previously complemented by wild-type gyra and gyrb plasmid-borne alleles and studied for their coresistance to imipenem (e. cambau, e. perani, c. dib, c. petinon, j. trias, and v. jarlier, antimicrob. agents chemother. 39:2248-2252, 1995). in the present study, we found mutations in type ii topoisomerase g ... | 1999 | 9869566 |
| sequence diversity of flagellin (flic) alleles in pathogenic escherichia coli. | to study the molecular evolution of flagellin, the protein subunit specifying flagellar (h) antigens, the flic genes from 15 pathogenic strains of escherichia coli were amplified by pcr and sequenced. comparison of flic sequences of h6 and h7 strains revealed that alleles have a mosaic structure indicating the occurrence of past horizontal transfer of dna segments between strains. the close similarity of h7 sequences also indicates the exchange of an entire flic h7 allele between distant clonal ... | 1999 | 9864325 |
| response to reactive nitrogen intermediates in mycobacterium tuberculosis: induction of the 16-kilodalton alpha-crystallin homolog by exposure to nitric oxide donors. | in contrast to the apparent paucity of mycobacterium tuberculosis response to reactive oxygen intermediates, this organism has evolved a specific response to nitric oxide challenge. exposure of m. tuberculosis to no donors induces the synthesis of a set of polypeptides that have been collectively termed nox. in this work, the most prominent nox polypeptide, nox16, was identified by immunoblotting and by n-terminal sequencing as the alpha-crystallin-related, 16-kda small heat shock protein, shsp1 ... | 1999 | 9864257 |
| cloning and characterization of a novel membrane-associated antigenic protein of helicobacter pylori. | infection by helicobacter pylori, a noninvasive bacterium, induces chronic leukocyte infiltration in the stomach by still largely unknown molecular mechanisms. we investigated the possibility that a membrane protein of h. pylori induces an inflammatory reaction in the subepithelial tissue of the stomach. by generating an expression library of h. pylori chromosomal dna and screening with rabbit antiserum raised to a membrane fraction of h. pylori and sera of infected patients, we cloned a 16.0-kd ... | 1999 | 9864228 |
| campylobacter jejuni-stimulated secretion of interleukin-8 by int407 cells. | incubation of int407 cells with various clinical isolates of campylobacter jejuni resulted in secretion of interleukin-8 (il-8) at levels ranging from 96 to 554 pg/ml at 24 h. the strains which produced the highest levels of il-8 secretion were 81-176 and bt44. induction of il-8 secretion required live cells of 81-176 and was dependent on de novo protein synthesis. site-specific mutants of 81-176, which were previously shown to be defective in adherence and invasion, resulted in reduced levels o ... | 1999 | 9864200 |
| campylobacter jejuni in foods. | 1998 | 9861962 | |
| campylobacter fetus surface layer proteins are transported by a type i secretion system. | the virulence of campylobacter fetus, a bacterial pathogen of ungulates and humans, is mediated in part by the presence of a paracrystalline surface layer (s-layer) that confers serum resistance. the subunits of the s-layer are s-layer proteins (slps) that are secreted in the absence of an n-terminal signal sequence and attach to either type a or b c. fetus lipopolysaccharide in a serospecific manner. antigenic variation of multiple slps (encoded by sapa homologs) of type a strain 23d occurs by ... | 1998 | 9851986 |
| the virulence plasmid of yersinia, an antihost genome. | the 70-kb virulence plasmid enables yersinia spp. (yersinia pestis, y. pseudotuberculosis, and y. enterocolitica) to survive and multiply in the lymphoid tissues of their host. it encodes the yop virulon, an integrated system allowing extracellular bacteria to disarm the cells involved in the immune response, to disrupt their communications, or even to induce their apoptosis by the injection of bacterial effector proteins. this system consists of the yop proteins and their dedicated type iii sec ... | 1998 | 9841674 |
| life below the gum line: pathogenic mechanisms of porphyromonas gingivalis. | porphyromonas gingivalis, a gram-negative anaerobe, is a major etiological agent in the initiation and progression of severe forms of periodontal disease. an opportunistic pathogen, p. gingivalis can also exist in commensal harmony with the host, with disease episodes ensuing from a shift in the ecological balance within the complex periodontal microenvironment. colonization of the subgingival region is facilitated by the ability to adhere to available substrates such as adsorbed salivary molecu ... | 1998 | 9841671 |
| effect of low-osmolality nutrient media on growth and culturability of campylobacter species. | the growth and culturability of campylobacter jejuni nctc 11351 and other campylobacters were examined in media having different osmolalities at a range of temperatures (4, 25, and 42 degreesc). the medium osmolalities used ranged from the osmolality of full-strength nutrient medium (modified campylobacter broth having an osmolality of around 254 mosmol) down to 96 mosmol. the following two methods were used to produce media having different osmolalities: dilution of the nutrient medium with dis ... | 1998 | 9835543 |
| rapid emergence of high-level resistance to quinolones in campylobacter jejuni associated with mutational changes in gyra and parc. | quinolone resistance in clinical isolates of campylobacter jejuni in sweden increased more than 20-fold at the beginning of the 1990s. resistance to 125 microgram of ciprofloxacin per ml in clinical isolates was associated with chromosomal mutations in c. jejuni leading to a thr-86-ile substitution in the gyra product and a arg-139-gln substitution in the parc product. | 1998 | 9835526 |
| high-level resistance to trimethoprim in clinical isolates of campylobacter jejuni by acquisition of foreign genes (dfr1 and dfr9) expressing drug-insensitive dihydrofolate reductases. | the pathogenic bacterium campylobacter jejuni has been regarded as endogenously resistant to trimethoprim. the genetic basis of this resistance was characterized in two collections of clinical isolates of c. jejuni obtained from two different parts of sweden. the majority of these isolates were found to carry foreign dfr genes coding for resistant variants of the dihydrofolate reductase enzyme, the target of trimethoprim. the resistance genes, found on the chromosome, were dfr1 and dfr9. in abou ... | 1998 | 9835491 |
| [reactive arthritis and erythema nodosum caused by campylobacter jejuni]. | 1998 | 9835163 | |
| cloning, sequencing, and characterization of the lipopolysaccharide biosynthetic enzyme heptosyltransferase i gene (waac) from campylobacter jejuni and campylobacter coli. | campylobacter jejuni and campylobacter coli are common causes of gastrointestinal disease and a proportion of c. jejuni infections have been shown to be associated with the guillain-barré syndrome. the waac gene from campylobacter coli, involved in lipopolysaccharide core biosynthesis, was cloned by complementation of a heptose-deficient strain of salmonella typhimurium, as judged by novobiocin sensitivity, lipopolysaccharide (lps)-specific phage sensitivity, and polyacrylamide-resolved lipopoly ... | 1998 | 9831648 |
| [the relationship of campylobacter jejuni subsp. jejuni enterotoxigenicity and the increase of camp and electrolyte changes in the rat intestine]. | small intestine alterations produced by the enterotoxigenic capacity of campylobacter jejuni subsp. jejuni are similar to the hydric, electrolytic and pathological changes caused by choleraic and thermolabile escherichia coli toxins. | 1998 | 9830743 |
| high frequency of metronidazole resistance among strains of campylobacter jejuni isolated from birds. | a total of 2157 strains of the enteropathogen campylobacter jejuni were examined for resistance to metronidazole (5 mg l-1). high rates of metronidazole resistance, between 82 and 100%, were observed amongst strains of avian origin, including commercially reared broiler chickens (90.1%, n = 314) and turkeys (92%, n = 100), as well as wild birds sampled from farms and coastal environments, such as starlings (82%, n = 50), and gulls (100%, n = 20). in comparison, isolates from bovine and ovine rum ... | 1998 | 9830138 |
| development of a multiplex pcr gene fingerprinting method using gyra and pfla polymorphisms to identify genotypic relatedness within campylobacter jejuni species. | the two genes gyra and pfla, whose sequence variability had been previously described, were evaluated separately for their potential value in discriminating strains of campylobacter jejuni. a single method was then developed by which the two loci were simultaneously amplified using a multiplex pcr procedure, and banding patterns were generated using a pre-selected set of restriction endonuclease enzymes. the method was applied to 18 strains of camp. jejuni from different poultry sources varying ... | 1998 | 9830118 |
| the bacillus subtilis nucleotidyltransferase is a trna cca-adding enzyme. | there has been increased interest in bacterial polyadenylation with the recent demonstration that 3' poly(a) tails are involved in rna degradation. poly(a) polymerase i (pap i) of escherichia coli is a member of the nucleotidyltransferase (ntr) family that includes the functionally related trna cca-adding enzymes. thirty members of the ntr family were detected in a search of the current database of eubacterial genomic sequences. gram-negative organisms from the beta and gamma subdivisions of the ... | 1998 | 9829937 |
| molecular characterization of a stress-inducible gene from lactobacillus helveticus. | a gene (htra) coding for a stress-inducible htra-like protein from lactobacillus helveticus cnrz32 was cloned, sequenced, and characterized. the deduced amino acid sequence of the gene exhibited 30% identity with the htra protein from escherichia coli; the putative catalytic triad and a pdz domain that characterize the htra family of known bacterial serine proteases were also found in the sequence. expression of the l. helveticus htra gene in a variety of stress conditions was analyzed at the tr ... | 1998 | 9829922 |
| measurement of motility of helicobacter pylori, campylobacter jejuni, and escherichia coli by real time computer tracking using the hobson bactracker. | (1) to make precise measurements and comparisons of various aspects of motility of three gastrointestinal pathogens, helicobacter pylori, campylobacter jejuni, and escherichia coli, in log phase growth; (2) to provide background information on motility data to study the influence of ph, viscosity, and chemotactic factors, thereby gaining a better understanding of bacterial pathogenesis. | 1998 | 9828825 |
| a foodborne outbreak of campylobacter jejuni (o:33) infection associated with tuna salad: a rare strain in an unusual vehicle. | we report a foodborne outbreak of campylobacter jejuni infection in a summer camp. outbreak-related cases occurred in 79 persons including 3 secondary cases in campers. campylobacter jejuni was isolated from stool specimens from 16 of 21 patients who submitted a sample; 13 viable isolates were serotyped and all were serotype o:33 (somatic o scheme) or hl:18 (heat-labile scheme), and biotype iii (lior scheme). this serotype is widely distributed geographically but rarely isolated from humans. sam ... | 1998 | 9825778 |
| a campylobacter outbreak associated with stir-fried food. | an outbreak of gastrointestinal illness affecting 12 of 29 customers of a 'hawaiian' theme restaurant specializing in stir-fried food occurred in cardiff, wales in february 1997. campylobacter jejuni serotype hs50 phage type 49 (pt49) was isolated from 5 cases. a total of 47 isolates of c. jejuni hs50 pt49 were identified from wales during 1997, of which 11 were isolated in late february or early march and from the cardiff area. in the outbreak, illness was associated with eating stir-fried chic ... | 1998 | 9825777 |
| genetic contribution of the tumor necrosis factor region in guillain-barré syndrome. | we studied genetic polymorphisms in the tumor necrosis factor (tnf) region in 81 japanese patients with guillain-barré syndrome (gbs) and 85 controls. a significantly higher frequency of the 100-base pair (bp) (tnfa2) allele of the tnfa microsatellite marker, which is associated with high tnf alpha production, existed in campylobacter jejuni-positive (cj+) gbs patients than in controls, suggesting the involvement of a genetic predisposition to high tnf alpha secretion in the development of c. je ... | 1998 | 9818939 |
| incidental finding of myxobolus spores (protozoa: myxozoa) in stool samples from patients with gastrointestinal symptoms. | myxozoan spores were detected in fecal samples from three patients presenting with abdominal pain and/or diarrhea. the spores were identical to those of myxobolus plectroplites, a previously described pathogen from the freshwater fish plectroplites ambiguus. all patients had recently eaten fish caught from local waters, and frozen fillets of such fish were found to be infected with m. plectroplites cysts. the passage of spores unchanged through the alimentary tract suggests they were incidental ... | 1998 | 9817910 |
| toxoplasma gondii-associated guillain-barré syndrome in an immunocompetent patient. | we report a case of guillain-barré syndrome associated with an acute, disseminated toxoplasmosis due to a new and unknown zymodeme (zymodeme 12) in an immunocompetent patient. the patient's condition improved with pyrimethamine and sulfadiazine. only the standard treatment for toxoplasmosis was effective, whereas intravenous immunoglobulins were ineffective. | 1998 | 9817908 |
| prevalence of astrovirus infection among chilean children with acute gastroenteritis. | the frequency of astrovirus infection in 456 chilean children with diarrhea was determined by enzyme-linked immunosorbent assay, reverse transcriptase pcr, and cell culture. astrovirus was detected in 16.5% of rotavirus-negative and 7% of rotavirus-positive samples obtained from emergency rooms or hospitals and in 11% of samples from day care centers. hast-1 was the predominant serotype identified. | 1998 | 9817899 |
| differentiation of campylobacter jejuni serotype o19 strains from non-o19 strains by pcr. | guillain-barré syndrome (gbs), a neurologic disease characterized by acute paralysis, is frequently preceded by campylobacter jejuni infection. serotype o19 strains are overrepresented among gbs-associated c. jejuni isolates. we previously showed that all o19 strains tested were closely related to one another by randomly amplified polymorphic dna (rapd) and restriction fragment length polymorphism analyses. rapd analysis demonstrated a 1.4-kb band in all o19 strains tested but in no non-o19 stra ... | 1998 | 9817874 |
| [toxic megacolon as a complication of campylobacter jejuni enterocolitis]. | we report the case of a previously healthy 53-year-old white male who developed an extraordinary complication of acute campylobacter jejuni colitis. toxic megacolon occurred while the patient was treated with a fluoroquinolone antibiotic and glucocorticoids, which were given for endoscopically suspected crohn's colitis. during the course of the disease no cause of colitis was found other than c. jejuni. despite the extreme dilatation, the patient was treated conservatively with parenteral nutrit ... | 1998 | 9816615 |
| epidemiologic observations on diarrhea developing in u.s. and mexican students living in guadalajara, mexico. | background: a previous study suggested that u.s. students who lived in mexico for 1 year had a risk of diarrhea intermediate between the rate for newly arrived u.s. students and mexican students; however, the study was not controlled for changes of risky behavior over time. methods: an analysis of acute diarrhea occurring among u.s. and mexican student groups living in guadalajara, mexico was conducted to explore the association of diarrhea developing during selected 28-day periods with length o ... | 1995 | 9815352 |
| bacterial enteric pathogens in uncooked foods in thai markets. | in developing countries, the morbidity and mortality rates of gastrointestinal tract infections from food borne bacteria have been difficult to establish. most studies have only been able to gather data prospectively from isolated geographic sources, rather than from large point-source epidemics. this study investigates the types of bacterial enteric pathogens found in food that was collected in a community in western thailand, where sporadic cases of hemolytic uremia syndrome and cholera have b ... | 1994 | 9815313 |
| application of conductance techniques to the detection of thermophilic campylobacter spp. in river water. | a study of basal medal identified campylobacter enrichment broth, with (ceb+) and without (ceb) antibiotic supplement, as a suitable medium for the detection and enumeration of campylobacter jejuni, c. coli and c. lari within aqueous samples via conductance methodology. despite apparent differences in conductivity profiles between species in the presence of antibiotics, no significant differences (p < 0.05) were detected between detection times for each species tested. ceb+ was successfully empl ... | 1998 | 9812369 |
| physical and genetic map of the pasteurella multocida a:1 chromosome. | a physical and genetic map of the pasteurella multocida a:1 genome was generated by using the restriction enzymes apai, ceui, and noti. the positions of 23 restriction sites and 32 genes, including 5 rrn operons, were localized on the 2.35-mbp single circular chromosome. this report presents the first genetic and physical map for this genus. | 1998 | 9811669 |
| identification and characterization of the fis operon in enteric bacteria. | the small dna binding protein fis is involved in several different biological processes in escherichia coli. it has been shown to stimulate dna inversion reactions mediated by the hin family of recombinases, stimulate integration and excision of phage lambda genome, regulate the transcription of several different genes including those of stable rna operons, and regulate the initiation of dna replication at oric. fis has also been isolated from salmonella typhimurium, and the genomic sequence of ... | 1998 | 9811652 |
| anxiogenic effect of subclinical bacterial infection in mice in the absence of overt immune activation. | challenge of animals with infectious microorganisms is well documented to affect a number of behavioral measures through activation of immune-neural mechanisms. in the present study, the ability of an infectious microorganism to directly alter behavioral responses in the absence of an overt immunologic response was examined. eight-week-old cf-1 male mice were infected orally with the gram-negative pathogen campylobacter jejuni in order to establish a subclinical infection that did not result in ... | 1998 | 9811366 |
| antagonistic activity against helicobacter infection in vitro and in vivo by the human lactobacillus acidophilus strain lb. | the purpose of the present study was to examine the activity of the human lactobacillus acidophilus strain lb, which secretes an antibacterial substance(s) against helicobacter pylori in vitro and in vivo. the spent culture supernatant (scs) of the strain lb (lb-scs) dramatically decreased the viability of h. pylori in vitro independent of ph and lactic acid levels. adhesion of h. pylori to the cultured human mucosecreting ht29-mtx cells decreased in parallel with the viability of h. pylori. in ... | 1998 | 9797324 |
| rrna stability in heat-killed and uv-irradiated enterotoxigenic staphylococcus aureus and escherichia coli o157:h7. | differentiation of viable cells from nonviable cells is of considerable importance in the development of methods to detect foodborne pathogens. to study the suitability of 16s rrna as an indicator of cell viability in nucleic acid-based detection assays, we examined rrna stability in two representative foodborne pathogens, escherichia coli o157:h7 and enterotoxigenic staphylococcus aureus, which were inactivated by extreme heat, moderate heat, and uv irradiation. cell death under all conditions ... | 1998 | 9797275 |
| [clinical utility of measurement for anti-gm1 and anti-gq1b antibodies]. | gangliosides, important constituents of the plasma membrane, are particularly abundant in the nervous system. some patients develop guillain-barré syndrome after the administration of bovine brain gangliosides. we previously showed existence of molecular mimicry between gm1 ganglioside and lipopolysaccharide of campylobacter jejuni isolated from the patients with guillain-barré syndrome, and that between gq1b and c. jejuni isolated from fisher's syndrome patients. moreover, the anti-ganglioside ... | 1998 | 9789308 |
| diversity in protein synthesis and viability of helicobacter pylori coccoid forms in response to various stimuli. | the viability of the coccoid forms of helicobacter pylori was evaluated by assessing protein synthesis. metabolic labeling studies showed the synthesis of proteins and the specific protein profiles of h. pylori coccoids produced under various conditions. harsh conditions such as aerobiosis and starvation (lack of horse serum) in the culture did not affect the synthesis of proteins in the coccoids. lowering of the ph to that of gastric secretions induced expression of several proteins in the cocc ... | 1998 | 9784573 |
| deletion of the major proteolytic site of the helicobacter pylori cytotoxin does not influence toxin activity but favors assembly of the toxin into hexameric structures. | the helicobacter pylori cytotoxin is proteolytically cleaved at a flexible hydrophilic loop into two subunits. deletion of the loop sequences had no effect on biological activity of the toxin in the hela cell vacuolation assay but favored the organization of the protein into hexameric rather than heptameric structures. | 1998 | 9784571 |
| the spectrum of antecedent infections in guillain-barré syndrome: a case-control study. | to determine which antecedent infections are specifically associated with the guillain-barré syndrome (gbs). | 1998 | 9781538 |
| [rapd typing of campylobacter jejuni and comparison with lior's or penner's serotyping system]. | campylobacter jejuni were isolated from 7 epidemic outbreaks (121 isolates), 15 patients with gastroenteritis, chicken meats (47 isolates) and chicken cecal contents (70 isolates). the isolates and one standard strain of c. jejuni jcm2013 were analysed by randomly amplified polymorphic dna method (rapd). total of 254 c. jejuni isolates were divided 68 different rapd types which included strains that did not to divided by lior's or penner's serotyping system. to compare the similarities of rapd p ... | 1998 | 9780736 |
| molecular mimicry in campylobacter jejuni lipopolysaccharides and the development of guillain-barré syndrome. | 1998 | 9780289 | |
| a reverse transcriptase polymerase chain reaction assay for the detection of thermophilic campylobacter spp. | a novel method was developed for the detection of thermophilic enteropathogenic campylobacters based on the detection of mrna using the reverse transcriptase polymerase chain reaction (rt-pcr). the rna extraction method, dnase treatment and rt-pcr assay were shown to be specific for mrna. the assay is specific for the thermophilic campylobacters campylobacter jejuni, campylobacter coli and campylobacter upsaliensis and restriction fragment length polymorphism (rflp) analysis of the 256 bp amplif ... | 1998 | 9778457 |
| antibodies against helicobacter pylori were detected in the cerebrospinal fluid obtained from patients with guillain-barré syndrome. | we examined the antibodies against helicobacter pylori proteins in the cerebrospinal fluid (csf) of 7 patients with guillain-barré syndrome (gbs). crude h. pylori antigens, fractionated heat shock protein (hsp), and urease b (ub) from h. pylori antigens were separated by sds-page. with western blot analysis, four of seven csf samples had several igg antibodies against h. pylori proteins, including hsp and ub. no cross reactivity against campylobacter jejuni was observed. these antibodies may be ... | 1998 | 9778269 |
| detection of point mutations associated with resistance of helicobacter pylori to clarithromycin by hybridization in liquid phase. | when the standard procedure for determining antibiotic susceptibility of bacteria is used, the results are delayed, especially for bacteria that grow slowly, such as helicobacter pylori. treatment for this bacterium may involve clarithromycin, a compound for which resistance has been associated with point mutations on the 23s rrna gene. this resistance is currently found in organisms isolated from 0 to 15% of patients and jeopardizes the success of the treatment. we have designed a test involvin ... | 1998 | 9774580 |