Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| expression of adhesion molecules (icam-1, lfa-3) on human epithelial cells (a549) after respiratory syncytial virus infection. | 1995 | 7542091 | |
| antigenic analysis of the f protein of the bovine respiratory syncytial virus: identification of two distinct antigenic sites involved in fusion inhibition. | from two independent fusions, fifteen mabs directed to the f protein of the bovine respiratory syncytial virus (brsv) were characterized by radio-immunoprecipitation assays. competition binding assays among these mabs identified two distinct antigenic sites (a and b) and one overlapping site (ab). all of the mabs specific to epitopes belonging to site a neutralized the infectivity of the virus in vitro and recognized human and bovine rsv strains. only two out of the five mabs directed to epitope ... | 1995 | 7541983 |
| molecular epidemiology of respiratory syncytial virus: a review of the use of reverse transcription-polymerase chain reaction in the analysis of genetic variability. | respiratory syncytial virus (rsv) is the major viral cause of lower respiratory tract disease (bronchiolitis and pneumonia) in babies and infants. infections with the virus occur as annual winter epidemics in temperate climates, placing considerable pressure on the provision of hospital beds. the virus is unusual in that it can reinfect individuals and it can infect babies despite the presence of maternal antibody. rsv has a negative sense nonsegmented rna genome and as such is liable to high le ... | 1995 | 7541747 |
| immunosuppressive drugs and hypertrophic cardiomyopathy. | 1995 | 7540242 | |
| current respiratory infections. | 1995 | 7532521 | |
| [some characteristics of circulation and respiratory viruses in the country]. | the 17-year observation of changes in the etiological pattern of acute respiratory diseases (ard) in the country has demonstrated that different types of adenoviruses are prevalent in the circulation on the whole territory. adenoviral infection was found to prevail among non-influenza infections in the ukraine, rsv infection in the north of the russian federation and in the far east; piconaviral infections caused by echo and coxsackievirus b in the urals and byelarus republic. however, some year ... | 1994 | 7532499 |
| [production of monoclonal antibodies to the human respiratory syncytial virus]. | the paper describes the procedure for producing monoclonal antibodies to human respiratory asyncytial virus using hybridoma technology. two groups of antibodies were identified in the study of their specific activity. the representatives of the first group preferably reacted with purified virus antigens. those of the second one binded mostly to unpurified virus antigens from the cultural fluid. the antibodies of these groups appear to be different in their epitope specificity. | 1994 | 7532487 |
| preparation of respiratory syncytial virus subgroup a and b antigens for enzyme immunoassay antibody detection. | a simplified method was described for purification of respiratory syncytial virus (rsv) subgroup a and b aimed to be used as antigens in enzyme immunoassay (eia). the titer of each rsv subgroup and the amount of protein was determined from the visible band in 45% sucrose gradient. the quality of prepared rsv subgroup antigens for eia was described in terms of the achievable final titer, the amount of protein, and eia criss-cross titration. the rsv subgroup a and b antigens, diluted as 1:100 (low ... | 1994 | 7532051 |
| linear antigenic and immunogenic regions of human respiratory syncytial virus n protein. | three linear antigenic regions on the n protein from human respiratory syncytial virus (rsv) subgroup a (strain a2) were identified by using peptides which reacted in elisa with sera from humans with recent or previous rsv infection. the determinants were localized within three hydrophilic regions of the protein: thr11 to gly30 (n3 peptide), ser231 to ala250 (n25 peptide) and thr371 to leu391 (n39 peptide). the site represented by the n39 peptide reacted with four subgroup a-specific mabs. there ... | 1995 | 7531217 |
| respiratory syncytial virus infections in malnourished children. | the prevalence of respiratory syncytial virus (rsv) infection among severely malnourished children was studied at the university of benin teaching hospital, benin city, nigeria at a time when the infection was known to be prevalent in the community. nasopharyngeal washings were obtained from subjects on admission and thereafter every 4 days until discharge. rsv was detected by elisa technique. of 20 well nourished children who served as controls, 11 were elisa-positive for rsv (55%). eight (16%) ... | 1994 | 7521627 |
| interleukin-8, interleukin-6, and soluble tumour necrosis factor receptor type i release from a human pulmonary epithelial cell line (a549) exposed to respiratory syncytial virus. | the release of interleukin-8 (il-8), interleukin-6 (il-6) and the soluble forms of the tumour necrosis factor receptor (stnf-r) from human pulmonary type ii-like epithelial cells (a549) after respiratory syncytial virus (rsv) infection was analysed. rsv infection alone induced a time- and rsv dose-dependent il-8 and il-6 release from a549 cells. furthermore, the soluble form of the tnf-ri was also secreted in a time- and rsv dose-dependent fashion. the soluble tnf-rii was not detected in the cel ... | 1994 | 7519169 |
| respiratory syncytial virus nucleocapsid protein (n) expressed in insect cells forms nucleocapsid-like structures. | the gene coding for the n protein of rsv strain long has been cloned and sequenced. it was introduced behind the polyhedrin promoter of the shuttle vector pvl941 and baculoviruses containing the n gene were constructed by homologous recombination. infection of spodoptera frugiperda 9 cells resulted in the production of large amounts of a protein similar in size and antigenicity to the authentic n protein. the baculovirus expressed n protein was concentrated in the cytoplasm of the insect cells a ... | 1994 | 7513931 |
| [diagnosis and treatment with traditional chinese medicine and western medicine in infantile pneumonia]. | 1993 | 7508774 | |
| enhanced pulmonary pathology associated with the use of formalin-inactivated respiratory syncytial virus vaccine in cotton rats is not a unique viral phenomenon. | the specificity of viral antigens in the formalin-inactivated, alum-precipitated respiratory syncytial virus (fi-rsv) vaccine in augmenting the pulmonary inflammatory response was evaluated. cotton rats were immunized with a fi-rsv vaccine derived from vero cells, a monkey cell line, or hep-2 cells, a human cell line. the fi-rsv/vero and the fi-rsv/hep-2 vaccines were prepared similarly to the original lot-100 fi-rsv vaccine that was associated with enhanced disease in the mid-1960s field trials ... | 1993 | 7508665 |
| loss of conserved cysteine residues in the attachment (g) glycoprotein of two human respiratory syncytial virus escape mutants that contain multiple a-g substitutions (hypermutations). | two escape mutants (r10c/1 and r10c/10) of the human respiratory syncytial (rs) virus long strain were selected after serial passage in the presence of monoclonal antibody c793 directed against the g glycoprotein. this antibody recognizes an epitope which is shared by all viruses of the two antigenic subgroups in which human rs virus isolates have been subdivided. the mutant viruses had lost most of the g protein conserved and subgroup-specific epitopes but maintained the strain-variable epitope ... | 1994 | 7507282 |
| conformational constraints of conserved neutralizing epitopes from a major antigenic area of human respiratory syncytial virus fusion glycoprotein. | to study the conformational requirements of epitopes from a conserved antigenic area (area ii) of respiratory syncytial (rs) virus fusion (f) glycoprotein, peptides of increasing length containing amino acids essential for these epitopes were synthesized. the synthetic peptides were tested for binding to a panel of neutralizing monoclonal antibodies (mabs) for this area as well as to rabbit hyperimmune and human convalescent antisera. antibody binding was dependent on peptide length; thus, a 61- ... | 1993 | 7506298 |
| binding of neutralizing monoclonal antibodies to regions of the fusion protein of respiratory syncytial virus expressed in escherichia coli. | cdna containing the entire coding sequence of the respiratory syncytial (rs) virus fusion (f) protein gene (574 amino acids) and two large psti restriction fragments, encoding amino acids 18 to 212 and 214 to 574, were expressed in escherichia coli as c-terminal chimeras with beta-galactosidase (beta-gal) in the pex expression vector system. a further cdna fragment, overlapping the psti restriction site and encoding amino acids 190 to 289, was derived by pcr and expressed in a similar manner. po ... | 1993 | 7506297 |
| bovine respiratory syncytial virus replicates minimally in bovine alveolar macrophages. | the interaction between two different bovine respiratory syncytial virus (brsv) strains and bovine alveolar macrophages (bams) was studied in vitro. bovine respiratory syncytial virus replicated minimally in bams and most of the virus produced remained cell-associated. approximately 1 out of 1,000 bams produced infectious virus, a number that further declined during the 7 days of culture. in contrast, bams exposed to bovine parainfluenza 3 virus (pi3v) produced high amounts of infectious virus. ... | 1995 | 7503690 |
| alteration of cytokine secretion and cytotoxicity of murine alveolar macrophages after in vitro infection with respiratory syncytial virus (rsv). | 1995 | 7502897 | |
| respiratory syncytial virus in infants and children. | respiratory syncytial virus (rsv) is a frequent cause of respiratory illness in infants and children, affecting almost 100% of children by the age of 3 years. mild rsv infection usually involves only the upper respiratory tract, while the lower respiratory tract is involved in severe disease. this article addresses the epidemiology of rsv infection, transmission of the disease, predisposing conditions, clinical course, diagnosis, and management. the majority of children can be managed on an outp ... | 1995 | 7501313 |
| respiratory syncytial virus--associated lower respiratory diseases in hospitalised pre-school children in ibadan. | an analysis of the features of lower respiratory diseases (lrd) associated with respiratory syncytial virus (rsv) in 21 children aged 1-23 months, indicated that the clinical features did not differ appreciably from those described for lrd due to other infective agents. it was however, remarkable that none of the children was grossly malnourished, neither was there any death. the final respiratory diagnoses were bronchiolitis alone (6 cases), bronchopneumonia (6 cases), empyema/pleural effusion ... | 1995 | 7495200 |
| viral respiratory infections and their role as public health problem in tropical countries (review). | acute respiratory infections (ari) are a major cause of morbidity and mortality throughout the world. data from the world health organization indicate that there are at least 2.2 million deaths from ari throughout the world each year. a considerable number of study have been performed in different countries to assess the etiological role of viruses in ari and is now clear that the majority of infections of the respiratory tract are caused by viruses. in tropical countries information on the vira ... | 1995 | 7495193 |
| respiratory syncytial virus, a rare cause of severe pneumonia following bone marrow transplantation. | mixed alveolar interstitial pneumonia is a much-feared complication in bone marrow transplant recipients because it carries a high mortality. many cases in which an etiological agent is identified are due to fungi or cytomegalovirus; rarely, other infectious agents such as pneumocystis carinii or (para)influenzavirus are involved. in this report we describe a patient who received intense chemotherapy followed by autologous bone marrow transplantation for relapse of testis seminoma and who develo ... | 1995 | 7492628 |
| safety and immunogenicity of the pfp vaccine against respiratory syncytial virus (rsv): the western blot assay aids in distinguishing immune responses of the pfp vaccine from rsv infection. | pfp-1 vaccine was evaluated in a randomized, controlled study in 47 rsv seropositive children. trivalent inactivated influenza virus (tiv) vaccine was the control. vaccine reactions were monitored, and bloods were obtained before vaccination, 4 weeks after vaccination, and at the end of the rsv season. respiratory illnesses were evaluated during the outbreak. neutralizing antibody (nt ab) assay to rsv, igg elisa to rsv proteins and a western blot assay were performed. acute reactions with the pf ... | 1995 | 7491817 |
| phosphorylation of ser232 directly regulates the transcriptional activity of the p protein of human respiratory syncytial virus: phosphorylation of ser237 may play an accessory role. | the phosphoprotein p of human respiratory syncytial virus (rsv) was expressed in eukaryotic cells in phosphorylated form. site-directed mutagenesis of the recombinant protein established ser232 as the major site of phosphorylation in vivo. phosphorylation of bacterially made p protein in vitro by purified casein kinase ii (ckii) resulted in the phosphorylation of ser237, whereas mainly ser232 was phosphorylated by a crude cell extract. the p kinase activity in the cell extract exhibited properti ... | 1995 | 7491765 |
| serological response to respiratory syncytial virus infection in pediatric patients with a comparison to immunofluorescence and virus isolation. | the serological response to respiratory syncytial virus (rsv) in 125 pediatric patients hospitalized with acute lower respiratory infection was investigated by enzyme linked immunosorbent assay (elisa) for specific immunoglobulin (ig) a, igg, and igm and complement fixation (cf) test. by elisa, a 4-fold rise in igg titre in paired sera was most commonly found, followed by a rise in iga and igm titres. investigation by elisa and cf leads to the suggestion that major cf activity against rsv antige ... | 1995 | 7488342 |
| bovine respiratory syncytial virus antibodies in non-bovine species. | to study the role of non-bovine species in the epidemiology of bovine respiratory syncytial virus (rsv) infections, sera obtained from 9 non-bovine animal species and from humans were examined for bovine rsv specific antibodies. sera were mainly from animals and humans which had been in contact with cattle. forty sera of each species were tested in an rsv specific whole virus elisa as well as in a peptide based elisa, that was developed to measure antibodies specific for bovine rsv. antibodies d ... | 1995 | 7487487 |
| evaluation of the antiviral activity of n-(phosphonoacetyl)-l-aspartate against paramyxoviruses in tissue culture and against respiratory syncytial virus in cotton rats. | n-(phosphonoacetyl)-l-aspartate (pala), a potent inhibitor of l-aspartic acid transcarbamoylase, was evaluated for cytotoxicity and antiviral activity against three different paramyxoviruses in tissue culture, and for antiviral efficacy and toxicity in vivo using a cotton rat-respiratory syncytial virus (rsv) model. significant in vitro cytotoxicity was observed in proliferating cultures of hep-2 (ic50 = 250 micrograms/ml) and vero cells (ic50 = 32 micrograms/ml), but was less evident in culture ... | 1995 | 7486959 |
| cold-passaged, temperature-sensitive mutants of human respiratory syncytial virus (rsv) are highly attenuated, immunogenic, and protective in seronegative chimpanzees, even when rsv antibodies are infused shortly before immunization. | a cold-passaged (cp) temperature-sensitive (ts) rsv mutant, designated rsv cpts-530, which possesses host-range mutations acquired during 52 passages at low temperature in bovine tissue culture and one or more ts mutations induced by chemical mutagenesis (shut-off temperature 39 degrees c) was found previously to be tenfold restricted in its replication in mice as compared to wild-type virus and stable genetically in nude mice. in the current study, we introduced additional attenuating mutations ... | 1995 | 7483808 |
| antibody responses by cattle after vaccination with commercial viral vaccines containing bovine herpesvirus-1, bovine viral diarrhea virus, parainfluenza-3 virus, and bovine respiratory syncytial virus immunogens and subsequent revaccination at day 140. | calves were vaccinated with four different commercial viral vaccines containing bovine herpesvirus-1 (bhv-1), bovine viral diarrhea (bvdv), parainfluenza-3 virus (pi-3v), and bovine respiratory syncytial virus (brsv) immunogens. for the initial vaccination certain vaccines were given twice (days 0 and 28), whereas other vaccines were given on day 0. the calves received another injection on day 140 with the vaccine originally given on day 0. the sera were collected at days 0, 7, 14, 21, 28, 42, 5 ... | 1995 | 7483787 |
| comparison of the t helper cell response induced by respiratory syncytial virus and its fusion protein in balb/c mice. | specific proliferative t-cell responses were induced in the lymph node cells (lnc) of mice immunised with a sucrose density gradient purified preparation of respiratory syncytial (rs) virus or an immunoaffinity purified preparation of the f glycoprotein. inhibition studies and flow cytometric analysis showed that the responding cell population were cd4+ t cells. the cytokines produced by virus-specific and f-specific cells were assessed using the ctll cell line. peak quantities of cytokine were ... | 1995 | 7483764 |
| [respiratory syncytial virus infection in adults]. | 1995 | 7481058 | |
| [rsv infection. international symposium from the 89th annual meeting of the german society for pediatrics. würzburg, september 9, 1993]. | 1993 | 7477026 | |
| update: respiratory syncytial virus activity--united states, 1995-96 season. | respiratory syncytial virus (rsv), a common cause of winter outbreaks of acute respiratory disease, is associated each year with an estimated 90,000 hospitalizations and 4500 deaths from lower respiratory tract disease in both infants and young children in the united states (1). outbreaks occur annually throughout the united states, and community activity usually peaks within 1 month of the national peak in january or february (figure 1) (2). rsv activity in the united states is monitored by the ... | 1995 | 7476847 |
| role of t-lymphocyte subsets in recovery from respiratory syncytial virus infection in calves. | the role of t-cell subsets in respiratory syncytial virus (rsv) infection was investigated by using monoclonal antibodies (mabs) to selectively deplete gnotobiotic calves of cd4+, cd8+, or wc1+ gamma delta t-cell receptor+ lymphocytes. injection of these mabs produced specific reductions of the target cell populations in the circulation and tissues. ten days after rsv infection, immunoglobulin m (igm), igg1, and iga antibodies were detected in sera and lung washings from control calves. depletio ... | 1995 | 7474075 |
| respiratory syncytial virus infection: ten-year follow-up. | studies within the last 20 years have shown that the incidence of asthma is increased in children who have been hospitalized with bronchiolitis due to respiratory syncytial virus. to evaluate such respiratory consequences in an ambulatory population, a follow-up study was done of 37 children who had respiratory syncytial virus infection documented at a community pediatric clinic in 1968 when they were younger than 4 years. this study, done ten years later by chart review and parental interview, ... | 1981 | 7471595 |
| nucleic acids of respiratory syncytial virus. | analysis of purified respiratory syncytial virus revealed that the virion rna was composed of 50s, 28s, 18s, and 4s species. the 18s and 28s species were presumed to represent host rrna since virus grown in actinomycin d-treated cells contained only 50s and 4s rnas. actinomycin d treatment stimulated production of infectious respiratory syncytial virus 5- to 10-fold. the 50s virion rna was shown to hybridize with polyadenylated mrna's isolated from infected cells, indicating that respiratory syn ... | 1980 | 7463558 |
| respiratory syncytial virus infections in madras. | 1980 | 7461760 | |
| experimental infection of calves with bovine respiratory syncytial virus (quebec strain). | an experiment was designed to evaluate the clinical, haematological, viral and serological aspects of bovine respiratory syncytial virus infection in calves. eleven calves were inoculated intranasally with bovine respiratory syncytial virus (quebec strain) in aerosol. clinical, haemotological and serological responses of the calves and virus shedding were monitored. the experimentally infected animals manifested moderate to severe signs of respiratory disease. the parameters used to evaluate the ... | 1980 | 7448628 |
| metabolic requirements for the maturation of respiratory syncytial virus. | the metabolic processes required for maturation of respiratory syncytial (rs) virus was determined by testing with metabolic inhibitors in hela cells that had been trypsinized 18 h p.i. although > 90% of the virus synthesized by that time remained cell-associated, treatment with trypsin inactivated at least 90% of the cell-associated virus. the trypsinized cells, when re-plated in virus growth medium, immediately resumed virus synthesis and this continued exponentially for at least 10 h, during ... | 1980 | 7441215 |
| outbreak of respiratory syncytial virus infection in the elderly. | 1980 | 7427656 | |
| [bronchiolitis]. | is an acute bronchial, obstructive disease of the infant caused mainly by the respiratory syncytial virus. it appears epidemically preceded by infections of the upper respiratory ducts, followed by coughing, dysnea, expiratory sibilants, suprasternal and subcostal during inspiration and radiologic evidences of choneking. in the differential diagnosis the physician must consider pulmonary dysgenesis, diaphragmatic hernia, congenital lobar emphysema, congenital cardiopathy, pneumothorax, obstructi ... | 1980 | 7426129 |
| the appearance of cell-bound ige in respiratory-tract epithelium after respiratory-syncytial-virus infection. | we studied the appearance of ige in the respiratory tract in 42 infants and young children with various forms of respiratory illness after infection by respiratory syncytial virus (rsv). ige was bound to exfoliated nasopharyngeal epithelial cells in most patients with rsv infection during the acute phase of infection, regardless of the form of illness. however, the continued presence of cell-bound ige was more common in patients with rsv-induced bronchiolitis or asthma than in patients with mild ... | 1980 | 7421946 |
| morphogenesis and structure of caprine respiratory syncytial virus. | cell cultures inoculated with caprine respiratory syncytial virus (rsv) were studied with light, fluorescent, and electron microscopy to determine the morphogenesis and structure of the virus. small syncytia were seen after 36 hours in culture. after 48 hours in culture, syncytia were large and numerous and pleomorphic cytoplasmic inclusions were seen. these inclusions were more pronounced and numerous later in the infection cycle. indirect immunofluorescence revealed a diffuse to granular cytop ... | 1980 | 7416970 |
| the stabilization and purification of respiratory syncytial virus using mgso4. | 1980 | 7414957 | |
| effects of ribavirin on respiratory syncytial virus in vitro. | ribavirin was demonstrated to have an antiviral effect on respiratory syncytial virus in vitro. a 50% reduction in plaque number was observed at concentrations of 3 or 10 micrograms of ribavirin per ml. this effect was observed when the drug was added as late as 12 h postinfection. at concentrations of greater than 10 micrograms of ribavirin per ml, the size of the syncytial plaque also noticeably decreased. ribavirin similarly decreased the number of infectious units released into the culture s ... | 1980 | 7396465 |
| concurrent respiratory syncytial virus and influenza a infections in the institutionalized elderly and chronically ill. | during a community outbreak of respiratory syncytial virus and influenza a/texas/77 infections, we investigated 71 cases of upper respiratory illness at a chronic disease hospital using a surveillance system plus viral and serologic studies. of the 32 patients with an etiologic diagnosis, seven had respiratory syncytial virus, 24 had influenza, and one had dual infections with respiratory syncytial virus and influenza. no definite etiologic diagnosis was made in the remaining 39 patients. a comp ... | 1980 | 7396314 |
| purification and polypeptides of respiratory syncytial virus. | 1980 | 7392979 | |
| how to contain respiratory syncytial virus. | 1980 | 7382084 | |
| systemic cell-mediated and antibody responses in infants with respiratory syncytial virus infections. | in order to investigate the possible role of immunity in lower respiratory tract disease of infants produced by respiratory syncytial (rs) virus, 18 hospitalized infants were tested for cell-mediated immune (cmi) responses in a whole blood culture assay utilizing a gamma emitting tracer, 5(125i) iodo-2'-deoxyuridine [125iudr] to quantitate cellular proliferative responses to virus antigen. class-specific antiviral antibody titres were determined in an indirect membrane immunofluorescence test. o ... | 1980 | 7373288 |
| the age dependence of respiratory syncytial virus growth in ferret lung can be shown in organ and monolayer cultures. | 1980 | 7371226 | |
| the antibody response to primary and secondary infection with respiratory syncytial virus: kinetics of class-specific responses. | serum antibody responses to primary and secondary infections with respiratory syncytial virus were determined in 67 hospitalized infants. responses in igg, igm, and iga immunoglobulin fractions were assayed using an indirect immunofluorescent technique. infection was confirmed by identification of rsv antigen in nasopharyngeal secretions using indirect immunofluorescence, and by recovery of the virus in tissue culture. igm specific antibody response was commonly observed within a few days of ons ... | 1980 | 7365579 |
| possible transmission by fomites of respiratory syncytial virus. | to test whether nosocomial spread of respiratory syncytial virus (rsv) could occur through contact with environmental surfaces contaminated by rsv-infected nasal secretions, survival in the environment of rsv isolated from media, pooled adult secretions, and secretions from hospitalized infants was examined. rsv in freshly obtained infant secretions was recovered from countertops for up to 6 hr, from rubber gloves for up to 1 1/2 hr, from cloth gowns and paper tissue for 30--45 min, and from ski ... | 1980 | 7365274 |
| decreased cell-mediated immunity to respiratory syncytial virus in multiple sclerosis. | we compared lymphocyte transformation to respiratory syncytial virus in patients with multiple sclerosis (ms) and in healthy controls. lymphocyte transformation was decreased in ms in the presence or absence of autologous plasma, adding to the evidence that cellular immunity to viral antigens may be broadly impaired in ms, and does not occur uniquely for any single virus. | 1981 | 7347997 |
| correlation of proliferation of lung epithelium with intramuscular sensitization and complement-fixing antibody to respiratory syncytial virus in the golden hamster. | intramuscular sensitization of hamsters with several forms of respiratory syncytial virus (rsv) caused proliferation of lung epithelium. in contrast, intranasal injection of live virus rarely resulted in this phenomenon. a correlation existed between proliferation of lung epithelium and presence of complement-fixing antibody, but not between lung disease and delayed skin reactions. complement-fixing antibody to rsv was found to be independent of the influence of the thymus. | 1981 | 7337963 |
| a reverse passive haemagglutination test for the detection of respiratory syncytial virus in nasal secretions from infants. | a reverse passive haemagglutination (rph) test has developed for the detection of respiratory syncytial (rs) virus in nasal secretions, taken from infants with acute respiratory illness. in the final form of the procedure, rs virus was detected in 24 of 25 samples positive for rs virus by tissue culture and/or fluorescence antibody staining and in two samples negative for rs virus by these techniques. the simplicity of the technique and the rapidity with which it may be performed together with i ... | 1981 | 7328421 |
| concentration of human respiratory syncytial virus using ammonium sulfate, polyethylene glycol or hollow fiber ultrafiltration. | human respiratory syncytial virus was concentrated by polyethylene glycol or ammonium sulfate precipitation as well as by hollow ultrafiltration. recoveries obtained were respectively 49.4%, 47.7%, and 75.2%; however, further analysis of these results by resuspension experiments showed that all the infectivity could be recovered from the different concentrates. the protein content of polyethylene glycol concentrates was much lower than those of ammonium sulfate or hollow fiber ultrafiltration. e ... | 1981 | 7328162 |
| acute fata; pneumonia in calves due to respiratory syncytial virus. | 1981 | 7324346 | |
| causes of pneumonia presenting to a district general hospital. | the incidence of bacterial, viral, mycoplasma, and rickettsial infections has been assessed prospectively in 210 adult patients with pneumonia who presented to a district hospital over a six-year period. one hundred and thirteen infective agents were detected in 103 patients. the agent most frequently detected was mycoplasma pneumoniae which accounted for 30 infections. a bacterial pathogen was found in 43 patients. streptococcus pneumoniae was the most common of these (24 patients); staphylococ ... | 1981 | 7314031 |
| respiratory syncytial virus antibodies in nonhuman primates and domestic animals. | 1981 | 7311470 | |
| detection of respiratory syncytial virus in nasopharyngeal secretions by inhibition of enzyme-linked immunosorbent assay. | an enzyme-linked immunosorbent assay developed for the demonstration of respiratory syncytial (rs) virus immunoglobulin g antibodies was used for the detection of rs virus in specimens of nasopharyngeal secretions (nps) obtained from children with acute respiratory disease. samples of nps were incubated with a fixed amount of standard serum (human serum antibodies to rs virus) before being added to the enzyme-linked immunosorbent assay test plate. a decrease in the optical density value determin ... | 1981 | 7309848 |
| monoclonal antibodies to respiratory syncytial virus: detection of virus neutralization and other antigen-antibody systems using infected human and murine cells. | monoclonal antibodies to human respiratory syncytial (rs) virus-specific antigens can be obtained without preliminary recourse to large-scale culture and purification of the virion. lytically infected human and persistently infected murine cultured cells expressing rs virus-specific cell surface and cytoplasmic antigens were substituted as priming immunogens and as substrates in solid-phase antibody radioimmunoassays. seven hybridoma clones secreting murine igg of either the gamma 1 or the gamma ... | 1981 | 7298794 |
| secretory antibody response to respiratory syncytial virus infection. | the secretory antibody response to primary and secondary infection with respiratory syncytial virus (rsv) in igg, igm and 11s iga immunoglobulin classes was determined using an indirect immunofluorescence antibody technique. nasopharyngeal secretions were collected after naturally acquired primary or secondary infection with rsv. immunoglobulin a responses to rsv were significantly greater in infants older than 6 months of age at the time of infection than in younger infants. reinfection with rs ... | 1981 | 7294005 |
| acute fatal pneumonia in calves due to respiratory syncytial virus. | 1981 | 7292939 | |
| antigen and polypeptide synthesis by temperature-sensitive mutants of respiratory syncytial virus. | a revised nomenclature for the polypeptides of respiratory syncytial (rs) virus has been devised on the basis of comparison of the long, a2 and rsn-2 strains by slab-gel electrophoresis. seven polypeptides, now designated vp200, vgp48, vpn41, vpp32, vpm27, vp25 and vp10, were observed in preparations of all three strains of rs virus, irrespective of the host cell of origin. in addition, a slowly migrating glycopolypeptide gp1 was prominent in partially purified rs virus of the long and a2 strain ... | 1981 | 7288405 |
| infectivity of respiratory syncytial virus by various routes of inoculation. | to understand the transmission of respiratory syncytial virus, we examined the frequency of infection in volunteers after inoculation by different routes with varying doses of virus. thirty-two adult volunteers received serial dilutions of a safety-tested live strain of respiratory syncytial virus instilled into nose, eye, or mouth. the highest inoculum, 5.2 log10 50% tissue culture infective dose (tcid50), was administered to four groups of four subjects each, by nose to one group, by eye to on ... | 1981 | 7287181 |
| characteristics of a persistent respiratory syncytial virus infection in hela cells. | 1981 | 7269238 | |
| acute fatal pneumonia in calves due to respiratory syncytial virus. | 1981 | 7269190 | |
| activation of complement by cells infected with respiratory syncytial virus. | the ability of respiratory syncytial virus (rsv)-infected he(p)-2 cells in culture to activate complement was investigated. after incubation of cells with various complement sources and buffer, binding of c3b to surfaces of infected cells was demonstrated by immunofluorescence with a double-staining technique. nonsyncytial and syncytial (i.e., fused, multinucleated) cells were separately enumerated. also, lysis of rsv-infected cells was assessed by lactic dehydrogenase release. in this system on ... | 1981 | 7263071 |
| acute fatal pneumonia in calves due to respiratory syncytial virus. | an acute pneumonia developed in 28 calves which had been housed together from one to two weeks of age. the clinical signs included pyrexia, tachypnoea, respiratory distress and coughing. some of the calves died. the pneumonia was characterised by an alveolitis with multinucleated syncytia, alveolar epithelial hyperplasia and bronchiolitis. interstitial emphysema was also present. fifteen of 19 calves examined serologically had rising neutralising antibody titres to respiratory syncytial virus; i ... | 1981 | 7257127 |
| bronchomammary axis in the immune response to respiratory syncytial virus. | the products of lactation from 26 nursing mothers were sequentially examined over several months for the presence or appearance of antibodies directed against respiratory syncytial virus. antiviral igm and igg were rarely identified in either colostrum or milk. rsv-specific iga was found in 75% (18/24) of specimens of colostrum; 40% (6/15) and 59% (4/7) of milk samples obtained at three and six months still contained specific iga antibody. the latter increase was felt to represent boosting of ex ... | 1981 | 7252673 |
| modes of transmission of respiratory syncytial virus. | 1981 | 7252646 | |
| effect of respiratory syncytial virus and virus-antibody complexes on the oxidative metabolism of human neutrophils. | the effect of respiratory syncytial virus (rsv) or mixtures of rsv and its specific antibody on the oxidative metabolic activity of human polymorphonuclear leukocytes was studied by the technique of luminol-dependent chemiluminescence. peripheral blood neutrophils obtained from normal healthy donors were used. rsv alone failed to induce any chemiluminescent response by the neutrophils. however, mixtures of rsv and rsv antibody-positive serum regularly elicited significant neutrophil chemilumines ... | 1981 | 7251140 |
| respiratory syncytial virus. | 1981 | 7249574 | |
| [prospects of respiratory syncytial virus vaccines]. | the latest vaccines for respiratory syncytial virus (rsv) are reviewed. a ts-1 variant is being studied in the attempt to complete the attenuation of the vaccine strain. further investigations of live rsv vaccine administered parenterally indicate that a target population over 6 months of age could benefit soon of this preparation. | 1980 | 7247482 |
| inhibition of respiratory syncytial virus-host cell interactions by mono- and diamidines. | several aromatic mono- and diamidines were found to block cell fusion induced by respiratory syncytial virus. the best inhibitors were able to achieve complete suppression of syncytium formation at a concentration of 1.0 microm. inhibition occurred in respiratory syncytial virus-infected hep-2 and cv-1 cells, but the same inhibitors were ineffective in preventing fusion induced by parainfluenza virus type 3. the fusion inhibitors did not reduce single-cycle virus yields, but did reduce multiple- ... | 1981 | 7247387 |
| legionnaires' disease combined with erythema multiforme in a 3-year-old boy. | a case of legionnaires' disease (ld) is described in a 3-year-old boy. he had fulminant disease with typical signs like bilateral pneumonia, gastrointestinal symptoms, and somnolence indicating involvement of the central nervous system. there was no premorbidity. an outstanding development was erythema multiforme, which has never previously been described in ld. the basic disease was caused by legionella pneumophila. this is evidenced by specific serum igm at admission and a subsequent significa ... | 1981 | 7246139 |
| [immunological studies of the relation between respiratory syncytial virus (rsv) and bronchiolitis]. | 1981 | 7242476 | |
| respiratory syncytial virus proteins: identification by immunoprecipitation. | the proteins of respiratory syncytial virus have not been clearly identified due to the lability of the virus and difficulties in its purification. we have pulse-labeled respiratory syncytial virus with [35s]methionine and [35s]cysteine and analyzed cell lysates by polyacrylamide gel electrophoresis. five 35s-labeled viral proteins ranging in molecular weight from 21,000 to 73,000 (vp73, vp44, vp35, vp28, and vp21) were easily discernable above background cellular proteins. treatment of the infe ... | 1981 | 7241656 |
| a two year prospective study of hospital-acquired respiratory virus infection on paediatric wards. | over a 24 month period on six paediatric wards of different designs 169 cases of possible hospital-acquired respiratory virus infection were investigated. a variety of viruses was isolated from 82 cases, the most common being respiratory syncytial virus, influenza, parainfluenza, adenoviruses and rhinoviruses. a further 73 children developed respiratory symptoms between 3 and 300 days after administration but viruses were not demonstrable by the techniques used. these children were thought to ha ... | 1981 | 7240735 |
| nosocomial respiratory syncytial viral infections. should gowns and masks be used? | the efficacy of infection control procedures utilizing gowns and masks in the control of nosocomial respiratory syncytial virus (rsv) infections was evaluated by comparing the rate of nosocomial rsv infections in infants and ward personnel during two sequential periods when gowns and masks were used (period 1) and not used (period 2). all patients (162) and staff (36) on our infants' ward were examined for signs of respiratory infection and had nasal washes obtained for viral isolation every two ... | 1981 | 7234784 |
| the development of balb/c cells persistently infected with respiratory syncytial virus: presence of ribonucleoprotein on the cell surface. | 1981 | 7232415 | |
| risk of respiratory syncytial virus infection for infants from low-income families in relationship to age, sex, ethnic group, and maternal antibody level. | the risk for hospitalization with respiratory syncytial virus infection during the first year of life was about five per 1,000 live births per year for infants born to low-income families in houston from 1975 to 1979. the risk varied depending upon the intensity of the epidemic for a given season, the month of birth of the infant, and the level of passively acquired maternal antibody at the time of birth. over 80% of the children hospitalized were less than 6 months of age; thus, most were born ... | 1981 | 7229749 |
| respiratory syncytial virus infections in oslo 1972--1978. ii. clinical and laboratory studies. | patient records from 422 children admitted to hospital during 6 winter outbreaks of rsv infection were examined retrospectively. bronchiolitis (255 patients) and pneumonia (98 patients) were the most common diagnoses. otitis media (76 patients) occurred as a complication to these conditions. children at risk for contracting severe illness caused by rsv were infants below the age of one year, more often boys than girls. of the children older than two years, 32% had already been diagnosed as asthm ... | 1980 | 7211357 |
| respiratory syncytial virus infections in oslo 1972--1978. i. virological and epidemiological studies. | respiratory syncytial virus (rsv) infection was diagnosed in 464 children admitted to hospital with acute respiratory disease from autumn 1972 to spring 1978. the virological surveillance was approximately 70%. as a rule, rsv infections occurred in distinct outbreaks in late autumn and winter together with a marked increase in the number of children admitted to hospital with acute lower respiratory tract disease. the incidence in children less than 1 year of age was about 10/1000 children/year. ... | 1980 | 7211356 |
| the possible role of respiratory syncytial virus and pasteurella spp in calf respiratory disease. | in a natural outbreak of respiratory disease during 1976, 31 of 43 calves showed moderately severe clinical signs which included pyrexia (maximum 42 degrees c) and tachypnoea. during the outbreak infection by respiratory syncytial virus (rsv) was demonstrated by isolation from nasopharyngeal swabs or by serology. pasteurella haemolytica or p multocida were isolated from the blood of four and five calves respectively. in the month before disease 65 per cent of the calves showed significant antibo ... | 1980 | 7210428 |
| respiratory syncytial virus and otitis media with effusion. | 1982 | 7201016 | |
| antibody content of rabbit milk and serum following inhalation or ingestion of respiratory syncytial virus and bovine serum albumin. | 1982 | 7200842 | |
| fatal haemorrhagic pneumonia in an adult due to respiratory syncytial virus and staphylococcus aureus. | 1982 | 7185980 | |
| respiratory syncytial virus activity in the united kingdom, 1979. | 1980 | 7185918 | |
| respiratory syncytial virus infections in children. | 1982 | 7183846 | |
| [encephalopathy producing antibodies against respiratory syncytial virus in the central nervous system in an adult woman]. | 1982 | 7164044 | |
| lack of relationship of community patterns of respiratory viral activity to systemic haemophilus influenzae disease in children. | the occurrence of epidemics of respiratory viral illness was monitored by virus isolations in a population of young children living in the greater nashville area over a 4-year period. epidemic disease due to influenza, respiratory syncytial virus and parainfluenza type 1 was present during discrete time blocks over this interval. occurrence of systemic haemophilus influenzae type b disease in the community was also documented by review of bacteriologic records of vanderbilt children's hospital o ... | 1982 | 7163030 |
| clinical evaluation of radioimmunoassay of nasopharyngeal secretions and serology for diagnosis of viral infections in children hospitalized for respiratory infections. | viral diagnosis was performed using radioimmunoassay (ria) for virus antigen in nasopharyngeal secretions (nps) and complement-fixation (cf) tests of paired sera from specimens of 90 children hospitalized for acute respiratory infection. major respiratory viruses sought for by both methods (adenoviruses, influenza a and b viruses, parainfluenza virus type 3, respiratory syncytial virus) were detected in 40 (44%) of the patients; 15% of the diagnoses were made by nps-ria alone. serologic diagnosi ... | 1982 | 7163028 |
| detection of respiratory syncytial virus in nasopharyngeal secretions by elisa: comparison with fluorescent antibody technique. | samples of nasopharyngeal secretions (nps) obtained from 140 infants and children with acute respiratory disease were examined for the presence of respiratory syncytial (rs) virus by elisa. an antiserum produced in rabbits against rs-virus polypeptides was used both as the "capture" antibody and as conjugate in a double-antibody sandwich technique. rs virus was detected by elisa in specimens of nps obtained from 72 (51%) of the patients. by comparison rs virus was shown to be present in 86 (61%) ... | 1982 | 7161598 |
| antibody against viruses in maternal and cord sera: specific antibody is concentrated on the fetal side of the circulation. | paired maternal and cord sera from 100 pregnancies were tested for antibodies against herpes simplex virus, measles virus and respiratory syncytial virus by complement fixation and for antibodies against rubella virus, influenza a virus and influenza b virus by haemagglutination-inhibition. for four viruses (herpes simplex, measles, respiratory syncytial and rubella) higher levels of antibody were found in cord than in maternal sera. there was no difference between maternal and cord serum titres ... | 1982 | 7130705 |
| radioimmunoassay of measles virus hemagglutinin protein g. | guinea pig and rabbit antisera from animals immunized with purified measles virus hemagglutinin (g) protein were used to establish a solid-phase four-layer radioimmunoassay for quantitative measurement of the g protein. the sensitive of the assay was 2 ng of purified g protein, and 200 micrograms of protein from uninfected vero cells neither decreased the sensitivity nor reacted non-specifically in the assay. radioimmunoassay standard dose-response curves were established and unknown values inte ... | 1982 | 7130334 |
| [detection of antibodies to respiratory syncytial virus in a neonate with multiple abnormalities]. | 1982 | 7125129 | |
| virus-cell interactions: nuclear magnetic resonance behaviour of intracellular water. | the water proton nmr spin-lattice relaxation time if measured in hep-2 cells in relation to the infection by different viruses (poliovirus type 1, coxsackievirus b-3, human adenovirus, measles virus, respiratory syncytial virus, herpes simplex virus, subtype 1, and vaccinia virus). the nmr properties of intracellular water allow the early detection and identification of viruses. a close relationship is shown between the nmr behavior and the mode of virus-cell interaction. | 1982 | 7121298 |
| development of antibody-dependent cell-mediated cytotoxicity in the respiratory tract after natural infection with respiratory syncytial virus. | antibody-dependent cell-mediated cytotoxicity (adcc) was measured in nasopharyngeal secretions collected from 42 infants and young children at various intervals after primary or secondary infection with respiratory syncytial virus. adcc was determined by specific immune release of 51cr from respiratory syncytial virus-infected hep-2 cell culture monolayers, with lymphocytes from adult volunteers as effector cells. specific adcc responses in nasopharyngeal secretions after primary infection were ... | 1982 | 7118247 |