Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| listeria monocytogenes bile salt hydrolase is a prfa-regulated virulence factor involved in the intestinal and hepatic phases of listeriosis. | listeria monocytogenes is a bacterial pathogen causing severe food-borne infections in humans and animals. it can sense and adapt to a variety of harsh microenvironments outside as well as inside the host. once ingested by a mammalian host, the bacterial pathogen reaches the intestinal lumen, where it encounters bile salts which, in addition to their role in digestion, have antimicrobial activity. comparison of the l. monocytogenes and listeria innocua genomes has revealed the presence of an l. ... | 2002 | 12180927 |
| identification of a second listeria seca gene associated with protein secretion and the rough phenotype. | we describe the identification and characterization of a second seca gene in listeria monocytogenes. this gene, termed seca2, is involved in smooth-rough phenotypic variation and seca2 expression contributes to bacterial virulence. spontaneous rough (r-) variants of l. monocytogenes grow in chains and form rough colonies on solid media. a subset of r-variants, classified here as type i, also shows reduced secretion of an autolysin, p60. we find that disruptions and in frame deletions in seca2 co ... | 2002 | 12180923 |
| french laboratory proficiency testing program for food microbiology. | the proficiency testing program in food microbiology (réseau d'analyses et d'echanges en microbiologie des aliments; raema), created in 1988, currently includes 440 participating laboratories. the program establishes proficiency in detection of salmonella and listeria monocytogenes, as well as quantitation of aerobic microorganisms, enterobacteriaceae, coliforms, escherichia coli, clostridium perfringens, coagulase-positive staphylococcus, and listeria monocytogenes. twice a year, 5 test samples ... | 2002 | 12180693 |
| identification and characterization of novel small rnas in the asps-yrvm intergenic region of the bacillus subtilis genome. | a novel rna species was isolated from bacillus subtilis, and its sequence was determined and mapped to its genetic position. this rna was termed bs190 rna from the length of its mature form (190 nt), and the gene encoding it is located within the asps-yrvm intergenic region of the b. subtilis genome. northern blotting revealed that the novel rna species is transcribed in vegetative cells as a larger precursor (bs201 rna, 201 nt). after transcription, the 5' end of the precursor is processed to g ... | 2002 | 12177353 |
| high-level resistance to class iia bacteriocins is associated with one general mechanism in listeria monocytogenes. | class iia bacteriocins may be used as natural food preservatives, yet resistance development in the target organisms is still poorly understood. in this study, the understanding of class iia resistance development in listeria monocytogenes is extended, linking the seemingly diverging results previously reported. eight resistant mutants having a high resistance level (at least a 10(3)-fold increase in mic), originating from five wild-type listerial strains, were independently isolated following e ... | 2002 | 12177330 |
| [the role of nitric oxide in listeria encephalitis of ruminants and in rats intracisternally infected with listeria monocytogenes]. | listeria monocytogenes is a gram-positive facultative intracellular bacteria which infects a wide range of hosts. in ruminants, infection with l. monocytogenes frequently causes encephalitis, which is usually fatal in sheep and goat, while cattle often recover with antibiotic therapy. since the role of no in the control of listeria is controversial, we have studied the expression of inos in the brains of cattle, sheep and goats which had succumbed to listeria encephalitis. inos was demonstrated ... | 2002 | 12174722 |
| activation of mucin exocytosis and upregulation of muc genes in polarized human intestinal mucin-secreting cells by the thiol-activated exotoxin listeriolysin o. | the secreted thiol-activated cytolysin listeriolysin o (llo) was responsible for l. monocytogenes-induced high-molecular glycoproteins (hmgs) exocytosis in cultured human mucosecreting ht29-mtx cells. by biochemical analysis we demonstrate that the majority of secreted hmgs in llo-stimulated cells are of mucin origin. in parallel, analysis of the expression of mucs genes showed that the transcription of the muc3, muc4 and muc12 genes encoding for membrane-bound mucins was increased in llo-stimul ... | 2002 | 12174086 |
| listeriolysin of listeria monocytogenes forms ca2+-permeable pores leading to intracellular ca2+ oscillations. | listeriolysin (llo) is a major virulence factor of listeria monocytogenes, a gram-positive bacterium that can cause life-threatening diseases. various signalling events and cellular effects, including modulation of gene expression, are triggered by llo through unknown mechanisms. here, we demonstrate that llo applied extracellularly at sublytic concentrations causes long-lasting oscillations of the intracellular ca2+ level of human embryonic kidney cells; resulting from a pulsed influx of extrac ... | 2002 | 12174083 |
| characterization of a bacteriocin produced by a newly isolated bacillus sp. strain 8 a. | the aim of this research was to investigate the production of bacteriocins by bacillus spp. isolated from native soils of south of brazil. | 2002 | 12174052 |
| pneumonia involving legionella pneumophila and listeria monocytogenes in an immunocompromised patient: an unusual coinfection. | recently, empirical therapy has been recommended for severe community-acquired pneumonia. we report the case of a 68-year-old prednisone-treated man with multiple myeloma who developed a fatal pneumonia due to legionella pneumophila and listeria monocytogenes confirmed by immunohistochemistry on postmortem lung sampling. involvement of the latter bacteria and association of two different pathogens are both highly uncommon features in pneumonia. the route of infection with l. monocytogenes is dis ... | 2002 | 12169753 |
| protective immunosurveillance of the central nervous system by listeria-specific cd4 and cd8 t cells in systemic listeriosis in the absence of intracerebral listeria. | the invasion of the cns by pathogens poses a major risk for damage of the highly vulnerable brain. the aim of the present study was to analyze immunological mechanisms that may prevent spread of infections to the cns. intraperitoneal application of listeria monocytogenes to mice induced infection of the spleen, whereas pathogens remained absent from the brain. interestingly, listeria-specific cd4 and cd8 t cells homed to the brain and persisted intracerebrally for at least 50 days after both pri ... | 2002 | 12165527 |
| the cell biology of listeria monocytogenes infection: the intersection of bacterial pathogenesis and cell-mediated immunity. | listeria monocytogenes has emerged as a remarkably tractable pathogen to dissect basic aspects of cell biology, intracellular pathogenesis, and innate and acquired immunity. in order to maintain its intracellular lifestyle, l. monocytogenes has evolved a number of mechanisms to exploit host processes to grow and spread cell to cell without damaging the host cell. the pore-forming protein listeriolysin o mediates escape from host vacuoles and utilizes multiple fail-safe mechanisms to avoid causin ... | 2002 | 12163465 |
| rip2: a novel therapeutic target for bacteria-induced inflammation? | 2002 | 12160628 | |
| bacteriological analysis of fresh produce in norway. | a total of 890 samples of fresh produce obtained from norwegian markets were examined in order to assess the bacteriological quality of the products and their potential public health risk. the samples comprised lettuce, pre-cut salads, growing herbs, parsley and dill, mushrooms and strawberries. the samples were analysed for the presence of thermotolerant coliform bacteria (tcb), escherichia coli o157, salmonella spp., listeria monocytogenes, staphylococcus spp., and yersinia enterocolitica. nei ... | 2002 | 12160079 |
| inlb, a surface protein of listeria monocytogenes that behaves as an invasin and a growth factor. | molecules from some pathogenic bacteria mimic natural host cell ligands and trigger engulfment of the bacterium after specifically interacting with cell-surface receptors. the leucine-rich repeat (lrr)-containing protein inlb of listeria monocytogenes is one such molecule. it triggers bacterial entry by interacting with the hepatocyte growth factor receptor (hgf-r or met) and two other cellular components: gc1q-r and proteoglycans. recent studies point to significant similarities between the mol ... | 2002 | 12154067 |
| a case report of sporadic ovine listerial menigoencephalitis in iowa with an overview of livestock and human cases. | a case of ovine listeriosis was examined in a flock of sheep. the index case was a male lamb, which was part of a flock of 85 sheep located in central iowa. because the sheep were raised on a premise where soybean sprouts were also cultivated for the organic foods market, the potential of a public health concern was addressed. to identify the source of contaminations, clinical and environmental samples were cultured for listeria monocytogenes. isolates were serotyped and analyzed using pulsed-fi ... | 2002 | 12152811 |
| stress-response sigma factor sigma(h) directs expression of the gltb gene encoding glutamate synthase in streptomyces coelicolor a3(2). | using the previously established escherichia coli two-plasmid system, we identified a promoter recognized by the streptomyces coelicolor stress-response sigma factor sigma(h). the promoter directed expression of the gltb gene, encoding a protein with considerable homology with large subunit of glutamate synthases. s1-nuclease mapping using rna prepared from s. coelicolor identified an identical transcription start point corresponding to the promoter. the level of the transcript from this promote ... | 2002 | 12151108 |
| irak-m is a negative regulator of toll-like receptor signaling. | toll-like receptors (tlrs) detect microorganisms and protect multicellular organisms from infection. tlrs transduce their signals through myd88 and the serine/threonine kinase irak. the irak family consists of two active kinases, irak and irak-4, and two inactive kinases, irak-2 and irak-m. irak-m expression is restricted to monocytes/macrophages, whereas other iraks are ubiquitous. we show here that irak-m is induced upon tlr stimulation and negatively regulates tlr signaling. irak-m prevented ... | 2002 | 12150927 |
| update on listeria monocytogenes infection. | listeria monocytogenes is a gram-positive bacillus that causes meningitis, encephalitis, bacteremia, and febrile gastroenteritis. most disease occurs in immunosuppressed individuals. recent seroepidemiologic studies show that the infection is foodborne. due to the increasing number of immunosuppressed individuals at risk for listeriosis, as well as the persistence of substantial foodborne outbreaks, l. monocytogenes has gained worldwide attention as an important pathogen. heightened surveillance ... | 2002 | 12149174 |
| transfer of microorganisms, including listeria monocytogenes, from various materials to beef. | the quantity of microorganisms that may be transferred to a food that comes into contact with a contaminated surface depends on the density of microorganisms on the surface and on the attachment strengths of the microorganisms on the materials. we made repeated contacts between pieces of meat and various surfaces (stainless steel and conveyor belt materials [polyvinyl chloride and polyurethane]), which were conditioned with meat exudate and then were contaminated with listeria monocytogenes, sta ... | 2002 | 12147503 |
| the pepr gene of lactobacillus sakei is positively regulated by anaerobiosis at the transcriptional level. | lactobacillus sakei is a lactic acid bacterium belonging to the natural flora of meat products. it constitutes the main flora of vacuum-packed meat and is largely used in western europe as a starter for the manufacturing of fermented sausages. this species is able to grow both under aerobiosis and anaerobiosis. in many technological processes involving it, oxygen is scarce. the aim of this study was to identify the major proteins affected by growth under anaerobiosis. using two-dimensional elect ... | 2002 | 12147484 |
| biochemical and genetic characterization of mundticin ks, an antilisterial peptide produced by enterococcus mundtii nfri 7393. | mundticin ks, a bacteriocin produced by enterococcus mundtii nfri 7393 isolated from grass silage in thailand, is active against closely related lactic acid bacteria and the food-borne pathogen listeria monocytogenes. in this study, biochemical and genetic characterization of mundticin ks was done. mundticin ks was purified to homogeneity by ammonium sulfate precipitation, sequential ion-exchange chromatography, and solid-phase extraction. the gene cluster (mun locus) for mundticin ks production ... | 2002 | 12147478 |
| inhibition of listeria monocytogenes in chicken cold cuts by addition of sakacin p and sakacin p-producing lactobacillus sakei. | to evaluate the potential of sakacin p and sakacin p-producing lactobacillus sakei for the inhibition of growth of listeria monocytogenes in chicken cold cuts, by answering the following questions. (i) is sakacin p actually produced in food? (ii) is sakacin p produced in situ responsible for the inhibiting effect? (iii) how stable is sakacin p in food? | 2002 | 12147066 |
| [initial antibiotic therapy in maternal-fetal infections which include ampicillin even in countries where listeriosis is an incidental disease]. | neonatal listeriosis is an exceptional disease in northern africa. hence, protocols for maternal-fetal infection treatment include only a third generation cephalosporin and an aminoside. this protocol does not take into account the possibility of listeria monocytogenes infection. we report a fatal case of neonatal listeriosis in tunisia. the use of first antibiotics in maternal-foetal infection must be reconsidered when lacking sufficient bacteriological data and include systematically ampicilli ... | 2002 | 12145955 |
| blood lines. | 2002 | 12145653 | |
| [rombencephalitis due to listeria monocytogenes. probable usefulness of dexamethasone associated with antibiotic treatment]. | infection of the central nervous system by listeria monocytogenes appears in most cases as acute meningitis which is indistinguishable from other types of acute meningitis. rombencephalitis is a rare form of neurolisteriosis, localized to the brainstem. the initial non specific symptoms may make early diagnosis difficult. | 2002 | 12134344 |
| viral and bacterial infections induce expression of multiple nk cell receptors in responding cd8(+) t cells. | nk cells express several families of receptors that play central roles in target cell recognition. these nk cell receptors are also expressed by certain memory phenotype cd8(+) t cells, and in some cases are up-regulated in t cells responding to viral infection. to determine how the profile of nk receptor expression changes in murine cd8(+) t cells as they respond to intracellular pathogens, we used class i tetramer reagents to directly examine ag-specific t cells during lymphocytic choriomening ... | 2002 | 12133970 |
| cross-presentation of listeria monocytogenes-derived cd4 t cell epitopes. | listeriolysin o (llo) mediates the evasion of listeria monocytogenes from the phagolysosome into the cytoplasm of the host cell. the recognition of infected cells by cd4 t cells is thought to be limited by the evasion of bacteria from the phagolysosome and also by the direct llo-mediated inhibition of cd4 t cell activation. to analyze the influence of these immunoevasive mechanisms on the antilisterial cd4 t cell response, the expansion of l. monocytogenes-specific cd4 and cd8 t cells was monito ... | 2002 | 12133966 |
| cross-reactive antigen is required to prevent erosion of established t cell memory and tumor immunity: a heterologous bacterial model of attrition. | induction and maintenance of t cell memory is critical for the control of intracellular pathogens and tumors. memory t cells seem to require few "maintenance signals," though often such studies are done in the absence of competing immune challenges. conversely, although attrition of cd8(+) t cell memory has been characterized in heterologous viral models, this is not the case for bacterial infections. in this study, we demonstrate attrition of t cell responses to the intracellular pathogen liste ... | 2002 | 12133940 |
| inhibition of listeriolysin o and phosphatidylcholine-specific production in listeria monocytogenes by subinhibitory concentrations of plant essential oils. | successful infection by listeria monocytogenes is dependent upon a range of bacterial extracellular proteins including a cytolysin termed listeriolysin o and phosphatidylcholine-specific phospholipase c. five plant essential oils--bay, clove, cinnamon, nutmeg and thyme--significantly reduced the production of listeriolysin o by l. monocytogenes. the greatest change was observed after culture with oil of thyme, which reduced haemolysis to 52.1 haemolytic units (hu)/ml compared with 99.8 hu/ml obs ... | 2002 | 12132773 |
| transferable tetracycline resistance in listeria monocytogenes from food in italy. | mechanisms of tetracycline resistance were investigated in two recent listeria monocytogenes isolates from food, with l. innocua 52p tet(r) as a control. tetracycline resistance was transferred conjugatively from all three strains to l. ivanovii and from one isolate and the control to enterococcus faecalis. molecular analysis demonstrated a chromosomal location for the tet determinant, which was identified as tetm in all cases. these studies are the first to show that l. monocytogenes from food ... | 2002 | 12132772 |
| bacteriological infections during the first hundred days of allogenic bone marrow transplantation--experience from oman. | allogeneic bone marrow transplantation (abmt) produces severe neutropenia lasting for days. during the first 100 days of allogeneic bmt bacterial infection is a major cause of mortality and morbidity. this is specially so till the patient engrafts with good neutrophil count (> 500/microl). nature of bacterial infection and its sensitivity pattern partly reflects the patient's own flora, hospital flora and the antibiotic usage pattern in the hospital and the community. hence although we know quit ... | 2002 | 12126346 |
| ena/vasp: solving a cell motility paradox. | modulating the concentration of the actin-binding protein ena/vasp within the lamellipodium of a migrating fibroblast results in marked changes in lamellipodium behaviour and actin network organization at the cell's leading edge. this can explain a cell motility paradox. | 2002 | 12123590 |
| prevalence of and resistance to anti-microbial drugs in selected microbial species isolated from bulk milk samples. | the prevalence of strains of staphylococcus aureus, coagulase-negative (cn) staphylococci, listeria monocytogenes, escherichia coli, enterococcus faecalis, e. faecium and bacillus cereus, was investigated in 111 bulk milk samples. staphylococcus aureus was isolated from 38 samples, cn staphylococci from 63 samples, e. coli from 49 samples, e. faecalis or e. faecium from 107 samples, and l. monocytogenes from two samples. bacillus cereus was not found in any of the samples and three samples were ... | 2002 | 12121041 |
| effects of inhaled ozone on pulmonary immune cells critical to antibacterial responses in situ. | the goal of this study was to examine effects from repeated exposure to ozone (o3) on immune cells involved in cell-mediated antibacterial responses in the lungs. rats exposed to 0.1 or 0.3 ppm o3 for 4 h/day, 5 days/wk, for 1 or 3 wk were analyzed for the ability to clear an intrapulmonary challenge with listeria monocytogenes or had their lungs processed to obtain pulmonary alveolar macrophages (pam) and lung-associated lymphocytes for analyses of select cell functions and surface marker expre ... | 2002 | 12119071 |
| colony-stimulating factor 1-dependent cells protect against systemic infection with listeria monocytogenes but facilitate neuroinvasion. | by using mice genomically lacking the mononuclear phagocytic growth factor colony-stimulating factor 1 and thereby deficient in macrophage and dendritic cell populations, we show that these cells play a dual role: they constitute a major defense against systemic infection but also facilitate cerebral bacterial invasion by listeria monocytogenes. | 2002 | 12117983 |
| mobilization of protein kinase c in macrophages induced by listeria monocytogenes affects its internalization and escape from the phagosome. | listeriolysin o (llo) and a phosphatidylinositol-specific phospholipase c (pi-plc) are known virulence factors of listeria monocytogenes in both tissue cultures and the murine model of infection. llo is a member of a family of pore-forming cholesterol-dependent cytotoxins and is known to play an essential role in escape from the primary phagocytic vacuole of macrophages. pi-plc plays an accessory role, in that pi-plc mutants are partially defective in escape. we have shown that both of these mol ... | 2002 | 12117979 |
| effect of acid adaptation on the fate of listeria monocytogenes in thp-1 human macrophages activated by gamma interferon. | in listeria monocytogenes the acid tolerance response (atr) takes place through a programmed molecular response which ensures cell survival under unfavorable conditions. much evidence links atr with virulence, but the molecular determinants involved in the reactivity to low phs and the behavior of acid-exposed bacteria within host cells are still poorly understood. we have investigated the effect of acid adaptation on the fate of l. monocytogenes in human macrophages. expression of genes encodin ... | 2002 | 12117947 |
| influence of catfish skin mucus on trisodium phosphate inactivation of attached salmonella typhimurium, edwardsiella tarda, and listeria monocytogenes. | this study examined the antimicrobial effectiveness of trisodium phosphate (tsp) on edwardsiella tarda, listeria monocytogenes, and salmonella typhimurium attached to catfish skin with and without mucus. salmonella typhimurium and e. tarda attached more readily to catfish skin than did l monocytogenes. at high inoculum levels (10(7) cfu/ml), tsp treatments (at 2 to 6%) for 10 min reduced bacterial counts of e. tarda by >2.5 to >3.3 log10 cfu per skin sample for firmly attached cells and by 3.5 t ... | 2002 | 12117249 |
| effects of uv irradiation on selected pathogens in peptone water and on stainless steel and chicken meat. | effects of intensity and processing time of 254 nm uv irradiation on listeria monocytogenes, escherichia coli o157:h7, and salmonella typhimurium were investigated. intensities measured at 5.08, 10.1, 15.2, and 20.3 cm from the light source were 1.000, 500, 250, and 150 microw/cm2, respectively. intensities of 250 or 500 microw/cm2 reduced all suspended pathogen cells in peptone water about 5 log cycles after 2 min and completely inactivated l. monocytogenes and e. coli o157:h7 after 3 min by re ... | 2002 | 12117248 |
| transfer of persistent listeria monocytogenes contamination between food-processing plants associated with a dicing machine. | the possibility of the transfer of persistent listeria monocytogenes contamination from one plant to another with a dicing machine was evaluated, and possible reasons for persistent contamination were analyzed. a dicing machine that diced cooked meat products was transferred from plant a to plant b and then to plant c. after the transfer of the dicing machine, l. monocytogenes pfge type i, originally found in plant a, was soon also found in plants b and c. this l. monocytogenes pfge type i cause ... | 2002 | 12117246 |
| development of a technique to quantify the effectiveness of enrichment regimes in recovering "stressed" listeria cells. | a rapid, reliable microwell plate method based on the most probable number (mpn) technique was used to determine the effectiveness of five enrichment regimes in the recovery and enumeration of listeria spp. cells from five seafood products. the products tested were chosen to reflect conditions under which cells were exposed to the "stresses" associated with a variety of food-processing techniques, such as treatments involving an ethanol-based marinade, lowered ph (acetic acid), heat, sugar and s ... | 2002 | 12117245 |
| relationship of cell surface charge and hydrophobicity to strength of attachment of bacteria to cantaloupe rind. | the cantaloupe melon has been associated with outbreaks of salmonella infections. it is suspected that bacterial surface charge and hydrophobicity may affect bacterial attachment and complicate bacterial detachment from cantaloupe surfaces. the surface charge and hydrophobicity of strains of salmonella, escherichia coli (o157:h7 and non-o157:h7), and listeria monocytogenes were determined by electrostatic and hydrophobic interaction chromatography, respectively. initial bacterial attachment to c ... | 2002 | 12117240 |
| inhibition of salmonella typhimurium and listeria monocytogenes in mung bean sprouts by chemical treatment. | this study was undertaken to compare the efficacies of chlorous acid (268 ppm), sodium hypochlorite (200 ppm), and lactic acid (2%) in eliminating total mesophilic microorganisms, salmonella typhimurium, and listeria monocytogenes on commercial mung bean sprouts immediately after treatment and during posttreatment refrigerated storage. treatment with sodium hypochlorite for 10 min did not reduce the total aerobic count. however, treatment with lactic acid and chlorous acid for 10 min initially r ... | 2002 | 12117239 |
| listeria monocytogenes meningitis: serotype distribution and patient characteristics in the netherlands, 1976-95. | two hundred and seven cases of listeria meningitis that occurred in the netherlands over 20 years were reviewed to study associations between listeria monocytogenes serotype, age, underlying disease, and outcome. the mean annual incidence per 100,000 population was 0.12 in 1981-90, decreasing to 0.07 in 1991-5. underlying disease was present in 50% of non-neonatal patients, most often haematological malignancy (15%) and the use of immunosuppressive therapy (14%). the meningitis-related case fata ... | 2002 | 12113484 |
| construction, characterization, and use of two listeria monocytogenes site-specific phage integration vectors. | two site-specific shuttle integration vectors were developed with two different chromosomal bacteriophage integration sites to facilitate strain construction in listeria monocytogenes. the first vector, ppl1, utilizes the listeriophage u153 integrase and attachment site within the comk gene for chromosomal insertion. ppl1 contains a useful polylinker, can be directly conjugated from escherichia coli into l. monocytogenes, forms stable, single-copy integrants at a frequency of approximately 10(-4 ... | 2002 | 12107135 |
| edible bioactive fatty acid-cellulosic derivative composites used in food-packaging applications. | to develop biodegradable packaging that both acts as a moisture barrier and as antimicrobial activity, nisin and stearic acid were incorporated into a hydroxy propyl methyl cellulose (hpmc) based film. fifteen percent (w/w hpmc) of stearic acid improved film moisture barrier. however, film mechanical resistance and film antimicrobial activity on listeria monocytogenes and staphylococcus aureus pathogenic strains were both reduced. this lower film inhibitory activity was due to interactions betwe ... | 2002 | 12105960 |
| cd94/nkg2 expression does not inhibit cytotoxic function of lymphocytic choriomeningitis virus-specific cd8+ t cells. | murine ag-specific cd8(+) t cells express various nk markers and nk inhibitory receptors that have been proposed to modulate immune responses. following acute infection of c57bl/6 and balb/cj mice with lymphocytic choriomeningitis virus (lcmv), we observed that ag-specific cd8(+) t cells expressed cd94/nkg2. only slight expression of ly49a and ly49c receptors was observed on np396-specific t cells, while all np396-specific t cells expressed the nkt cell marker u5a2-13 ag. expression of cd94/nkg2 ... | 2002 | 12097371 |
| neonatal lead exposure potentiates sickness behavior induced by listeria monocytogenes infection of mice. | the effects of lead (pb) administration on infection-induced decreases in water intake, food intake, and body weight gain have been assessed as manifestations of sickness behavior using a balb/c mouse model. pb acetate (0.5 mm) was administered via drinking water to dams from day 0 postpartum to weaning and to mouse pups after weaning until sacrifice. at 22 days after birth, young mice were infected with listeria monocytogenes. mice with blood pb levels of less than 25 microg/dl exhibited enhanc ... | 2002 | 12096892 |
| regulation of the expression of mouse tap-associated glycoprotein (tapasin) by cytokines. | the expression of antigen presenting mhc class i molecules can be enhanced through cytokines, e.g. upon infection with bacteria or viruses, either directly by enhancing class i gene transcription or by increasing the amounts of accessory proteins of the loading complex. tapasin plays a significant role in the peptide loading of class i molecules. here, we describe recognition motifs of cytokine inducible transcription factors in the promoter region of the mouse tapasin gene, most of them cluster ... | 2002 | 12095710 |
| spontaneous listeria monocytogenes peritonitis: a population-based study of 13 cases collected in spain. | we aimed to assess the incidence, demographic data, clinical features, and outcome of peritoneal infections due to listeria monocytogenes in individuals with cirrhosis. | 2002 | 12094874 |
| postpackage pasteurization of ready-to-eat deli meats by submersion heating for reduction of listeria monocytogenes. | a mixed cocktail of four strains of listeria monocytogenes was resuspended in product purge and added to a variety of ready-to-eat (rte) meat products, including turkey, ham, and roast beef. all products were vacuum sealed in shrink-wrap packaging bags, massaged to ensure inoculum distribution, and processed by submersion heating in a precision-controlled steam-injected water bath. products were run in pairs at various time-temperature combinations in either duplicate or triplicate replications. ... | 2002 | 12092729 |
| behavior of listeria monocytogenes inoculated on cantaloupe surfaces and efficacy of washing treatments to reduce transfer from rind to fresh-cut pieces. | attachment and survival of listeria monocytogenes on external surfaces (rind) of inoculated cantaloupe, resistance of the surviving bacteria to chlorine or hydrogen peroxide treatments, transfer of the pathogen from unsanitized and sanitized rinds to fresh-cut tissues during cutting and growth, and survival of l. monocytogenes on fresh-cut pieces of cantaloupe were investigated. surface treatment with 70% ethanol to reduce the native microflora on treated melon, followed by immersion in a four-s ... | 2002 | 12092724 |
| multidrug resistant neonatal sepsis in peshawar, pakistan. | to investigate the spectrum of organisms causing neonatal sepsis in peshawar, pakistan and to assess their sensitivity to various groups of drugs. | 2002 | 12091293 |
| hospital-acquired listeriosis. | we report four cases of listeriosis that occurred over a two-month period in north east england. due to the apparent nosocomial acquisition of infection and the clustering of cases in time and place, extended epidemiological investigation was performed and the outbreak was traced to a caterer who was providing sandwiches for hospital shops. we discuss the difficulties in preventing food-borne listeriosis in the hospital setting. | 2002 | 12090802 |
| [spontaneous bacterial peritonitis caused by listeria monocytogenes]. | 2002 | 12090069 | |
| positive selection of mhc class ib-restricted cd8(+) t cells on hematopoietic cells. | unlike conventional cd8(+) t cells, major histocompatibility complex (mhc) class ib-restricted cd8(+) t cells show an activated phenotype in uninfected mice and respond rapidly to foreign invaders. the underlying factors that contribute to these differences are not well understood. we show here that the activated phenotype of mhc class ib-restricted cd8(+) t cells was partially acquired as a result of interactions in the thymus and reflected an increased capacity to be selected via interactions ... | 2002 | 12089507 |
| prevalence and fingerprinting of listeria monocytogenes strains isolated from raw whole milk in farm bulk tanks and in dairy plant receiving tanks. | the incidence of listeria species in raw whole milk from farm bulk tanks and from raw milk in storage at a swedish dairy plant was studied. listeria monocytogenes was found in 1.0% and listeria innocua was found in 2.3% of the 294 farm bulk tank (farm tank) milk specimens. one farm tank specimen contained 60 cfu of l. monocytogenes ml(-1). l. monocytogenes was detected in 19.6% and l. innocua was detected in 8.5% of the milk specimens from the silo receiving tanks at the dairy (dairy silos). mor ... | 2002 | 12089016 |
| characterization of a listeria monocytogenes scott a isolate with high tolerance towards high hydrostatic pressure. | an isolate of l. monocytogenes scott a that is tolerant to high hydrostatic pressure (hhp), named ak01, was isolated upon a single pressurization treatment of 400 mpa for 20 min and was further characterized. the survival of exponential- and stationary-phase cells of ak01 in aces [n-(2-acetamido)-2-aminoethanesulfonic acid] buffer was at least 2 log units higher than that of the wild type over a broad range of pressures (150 to 500 mpa), while both strains showed higher hhp tolerance (piezotoler ... | 2002 | 12088993 |
| cd8(+) t cell responses: it's all downhill after their prime. | 2002 | 12087413 | |
| on the specificity of pcr detection of listeria monocytogenes in food: a comparison of published primers. | a total of nine pairs of primers, seven previously published and two newly developed, have been assayed for pcr detection of listeria monocytogenes in food. they have been tested for specificity on a total of 72 strains including reference and food isolates belonging to l. monocytogenes and other species in the genus. first of all, a polyphasic approach has been carried out in order to establish a reference strain collection. they were biochemically and genetically characterized by api-lis and r ... | 2002 | 12086177 |
| bactericidal group iia phospholipase a2 in serum of patients with bacterial infections. | group iia phospholipase a2 (pla2-iia) is a newly recognized antibacterial acute phase protein. the concentration of pla2-iia increases up to 500-fold in the blood plasma of patients with severe acute diseases, compared with healthy persons. despite numerous studies, the exact roles of this enzyme in human diseases are unknown. this study investigated the antibacterial properties of pla2-iia in human acute phase serum. pla2-iia in serum samples of patients with bacterial infections was capable of ... | 2002 | 12085323 |
| [clinico-epidemiological study of neurolisteriosis in the province of segovia]. | listeria monocytogenes is a microorganism with marked tropism towards the central nervous system. one of the commonest forms of presentation is as a meningeal infection. the objective of this study is to describe the epidemiological, clinical and laboratory characteristics and therapeutic implications. | 2002 | 12080487 |
| listeriosis in recipients of allogeneic blood and marrow transplantation: thirteen year review of disease characteristics, treatment outcomes and a new association with human cytomegalovirus infection. | listeriosis is uncommon in recipients of allogeneic blood, marrow and organ transplantation. six patients with systemic listeria monocytogenes infection during 1985-1997 at bone marrow transplantation service, memorial sloan-kettering cancer center are described. in two male and four female patients, the median duration from transplantation to isolation of l. monocytogenes was 62.5 (range 29 to 821) days. among five allogeneic marrow transplant recipients, four (80%) received hla antigen matched ... | 2002 | 12080357 |
| osmoprotection by carnitine in a listeria monocytogenes mutant lacking the opuc transporter: evidence for a low affinity carnitine uptake system. | a deletion mutant of listeria monocytogenes lacking opuc, an abc transporter responsible for the uptake of the compatible solute carnitine, was constructed and carnitine transport assays confirmed that carnitine transport was defective in this mutant. however, the mutant retained the ability to derive osmoprotection from carnitine, suggesting the presence of a second uptake system for this compatible solute. measurement of intracellular carnitine pools during balanced growth confirmed that the o ... | 2002 | 12076811 |
| similar listeria monocytogenes pulsotypes detected in several foods originating from different sources. | the purpose of the study was to obtain fingerprinting data of listeria monocytogenes strains isolated in various foods to determine possible associations of strains with product type, producer, country or isolation time. two hundred and ninety-five l. monocytogenes strains originating from food items of 41 producers of 10 countries were characterized by pulsed-field gel electrophoresis (pfge) typing. combination of asci and apai macrorestriction patterns (mrp) yielded 66 different pulsotypes. te ... | 2002 | 12076041 |
| a semi-quantitative seafood safety risk assessment. | as part of a semi-quantitative risk assessment of 10 seafood hazard/product combinations, a risk assessment tool was used to generate a risk ranking. the tool is in a spreadsheet software format and provides a risk estimate, which is scaled between 0 and 100, where 0 represents no risk and 100 represents all meals containing a lethal dose of the hazard. a full description of the tool is contained in ross and sumner (this issue). based on their ranking, seafoods in australia fell into three risk ... | 2002 | 12076038 |
| numbers and types of microorganisms in vacuum-packed cold-smoked freshwater fish at the retail level. | fifty-four packages (each one belonging to a different lot) of vacuum-packed cold-smoked salmon (30) and trout (24) produced by six spanish smokehouses were obtained at retail level after 3 weeks storage at 2+/-1 degrees c. sensorial, chemical, physicochemical and microbiological characteristics were examined. overall, ph, a(w), salt content in water phase, aerobic plate counts at 30 and 25 degrees c. levels of enterobacteriaceae, lactic acid bacteria (lab), fungi and presumptive aeromonads and ... | 2002 | 12076034 |
| molecular typing of listeria monocytogenes. | 2002 | 12073828 | |
| spontaneous bacterial peritonitis due to listeria monocytogenes in a patient with primary pulmonary hypertension. | 2002 | 12072948 | |
| [survey of the presence of bacterial pathogens in foods sold at retail stores in the city of cassino]. | the presence of bacterial food pathogens was evaluated in 154 food samples collected from supermarkets and butchers in the city of cassino (south-central italy). food pathogens were identified in 17.5% of the total food samples. in the raw meat samples, 24.6% tested positives for listeria monocytogenes, 4.3% for salmonella and 2.9% for escherichia coli o157. y. enterocolitica, only investigated in pork meat, was identified in 7.4% of the samples. in poultry, l. monocytogenes was identified in 55 ... | 2002 | 12070904 |
| tyrosine 759 of the cytokine receptor gp130 is involved in listeria monocytogenes susceptibility. | interleukin-6 family cytokines have been implicated in adaptive and innate immunity, hematopoiesis, and inflammation. this cytokine family shares a signal-transducing receptor subunit called gp130. gp130(f759/f759) knockin mice carry a point mutation at the shp2-binding site of gp130 due to the replacement of tyrosine-759 (y759 for human gp130) with phenylalanine (f). to explore the effect of this point mutation on the host response to bacterial infection, gp130(f759/f759) knockin mice were infe ... | 2002 | 12070777 |
| comparative intracellular (thp-1 macrophage) and extracellular activities of beta-lactams, azithromycin, gentamicin, and fluoroquinolones against listeria monocytogenes at clinically relevant concentrations. | the activities of ampicillin, meropenem, azithromycin, gentamicin, ciprofloxacin, and moxifloxacin against intracellular hemolysin-positive listeria monocytogenes were measured in human thp-1 macrophages and were compared with the extracellular activities observed in broth. all extracellular concentrations were adjusted to explore ranges that are clinically achievable in human serum upon conventional therapy. in broth, ampicillin, meropenem, and azithromycin were only bacteriostatic, whereas gen ... | 2002 | 12069960 |
| the occurrence of pathogenic listeria monocytogenes and antibodies against listeriolysin-o in buffaloes. | the occurrence of listeria monocytogenes in meat and milk samples, and antilisteriolysin o (allo) antibodies in sera of buffaloes were studied. isolation of the pathogen was attempted from the samples by selective enrichment in university of vermont medium and plating onto dominguez-rodriguez isolation agar. the pathogenicity of the isolates was tested by christie, atkins, munch petersen test and mouse inoculation test. of 167 meat samples 2.4 and 10.17% were positive for l. monocytogenes and li ... | 2002 | 12069270 |
| listerial endocarditis in a penicillin-allergic woman successfully treated with a combination of 4 drugs. | a 69-y-old woman with bioprosthetic endocarditis due to listeria monocytogenes developed an allergic reaction after beginning ampicillin treatment. she was cured with the combination of trimethoprim-sulfamethoxazole, rifampicin and teicoplanin. no immune deficiency was found in the patient. | 2002 | 12069025 |
| ifngamma and tnfalpha account for a pro-clonogenic activity secreted by activated murine peritoneal macrophages. | in the present study, we found that murine peritoneal macrophages elicited by bcg or listeria monocytogenes release into the media an activity capable of stimulating the lung colonization as well as the expression of mhc class i antigens in b16 melanoma cells. a similar activity has previously been found in media conditioned by corynebacterium parvum-elicited macrophages. analysis by gel filtration chromatography of media conditioned by corynebacterium parvum-, bcg- or listeria monocytogenes-eli ... | 2002 | 12067206 |
| sigma b contributes to prfa-mediated virulence in listeria monocytogenes. | transcription of the listeria monocytogenes positive regulatory factor a protein (prfa) is initiated from either of two promoters immediately upstream of prfa (prfap(1) and prfap(2)) or from the upstream plca promoter. we demonstrate that prfap(2) is a functional sigma(b)-dependent promoter and that a sigb deletion mutation affects the virulence phenotype of l. monocytogenes. thus, the alternative sigma factor sigma(b) contributes to virulence in l. monocytogenes. | 2002 | 12065541 |
| safety and shedding of an attenuated strain of listeria monocytogenes with a deletion of acta/plcb in adult volunteers: a dose escalation study of oral inoculation. | listeria monocytogenes is an intracellular bacterial pathogen which causes bacteremia and has a tropism for the central nervous system and a propensity to cause maternofetal infection. l. monocytogenes has been shown to be an effective prophylactic and a therapeutic vaccine vector for viral and tumor antigens in animal models. l. monocytogenes mutants lacking the acta protein, which is essential for intracellular movement, are attenuated but retain immunogenicity in mice. given the pathogenic po ... | 2002 | 12065500 |
| the changing epidemiology of bacterial meningitis and invasive non-meningitic bacterial disease in scotland during the period 1983-99. | we reviewed population-based laboratory reports of invasive meningococcal, pneumococcal, haemophilus influenzae, group b streptococcus (gbs) and listeria monocytogenes isolates in order to examine the changing epidemiology of meningitis and invasive non-meningitic disease (inmd) caused by these 5 pathogens in the 2 periods before (1983-91) and after (1992-99) routine use of h. influenzae type b conjugate vaccine (hib) in scotland. neissieria meningitidis was the most common cause of meningitis, ... | 2002 | 12064693 |
| prevalence and risk factors for contamination with listeria monocytogenes of imported and exported meat and fish products in switzerland, 1992-2000. | a total of 2053 import and 164 export samples from 425 production plants were examined over a 9-year period (1992-2000) for the presence of listeria monocytogenes (l. monocytogenes) in switzerland. overall, 282 samples (12.2%) and 85 plants (20.5%) harbored the pathogen. the highest isolation risk was for marinated fish (38%); the lowest was in cured- and dried-meat products. unconditional fixed-effect logistic regression was used to identify the main hazards associated with the presence of l. m ... | 2002 | 12062517 |
| programmed contraction of cd8(+) t cells after infection. | the extent of infection and rate of pathogen clearance are thought to determine both the magnitude of antigen-specific cd8(+) t cell expansion and the ensuing contraction to a stable number of memory cells. we show that cd8(+) t cell expansion after listeria monocytogenes infection was primarily dependent on the initial infection dose or amount of antigen displayed, and was also influenced by the rate of pathogen clearance. however, the onset and kinetics of cd8(+) t cell contraction after l. mo ... | 2002 | 12055624 |
| assessment of the pathogenic potential of two listeria monocytogenes human faecal carriage isolates. | two human faeces carriage isolates of listeria monocytogenes (h1 and h2) were compared to reference strains (scotta and lo28) with regard to their lethality in 14-day-old chick embryos, their haemolytic and phospholipase (phosphatidylcholine-phospholipase c and phosphatidylinositol-phospholipase c) activities and their invasiveness towards caco-2 cells. experimental infection of chick embryos allowed discrimination of the strains into those exhibiting high virulence (scotta and h2), those exhibi ... | 2002 | 12055305 |
| organ-specific cd4+ t cell response during listeria monocytogenes infection. | the immune response against the intracellular bacterium listeria monocytogenes involves both cd4(+) and cd8(+) t cells. we used the mhc class ii-presented peptide listeriolysin(189-201) to characterize the organ-specific cd4(+) t cell response during infection. systemic listeriosis resulted in a strong peptide-specific cd4(+) t cell response with frequencies of 1/100 and 1/30 cd4(+) splenocytes at the peak of primary and secondary response, respectively. this response was not restricted to lymph ... | 2002 | 12055256 |
| biochemical characterisation and genetic analysis of aureocin a53, a new, atypical bacteriocin from staphylococcus aureus. | aureocin a53 is produced by staphylococcus aureus a53. it is encoded on a 10.4 kb plasmid, prj9, and is active against listeria monocytogenes. aureocin a53 is a highly cationic 51-residue peptide containing ten lysine and five tryptophan residues. aureocin a53 was purified to homogeneity by hydrophobic-interaction, cation-exchange, and reverse-phase chromatography. maldi-tof mass spectrometry yielded a molecular mass of 6012.5 da, which was 28 da higher than predicted from the structural gene se ... | 2002 | 12054867 |
| reduction of iron by extracellular iron reductases: implications for microbial iron acquisition. | the extracellular enzymatic reduction of iron by microorganisms has not been appropriately considered. in this study the reduction and release of iron from ferrioxamine were examined using extracellular microbial iron reductases and compared to iron mobilization by chemical reductants, and to chelation by edta and desferrioxamine. a flavin semiquinone was formed during the enzymatic reduction of ferrioxamine, which was consistent with the 1 e(-) reduction of iron by an enzyme. the rates for the ... | 2002 | 12054438 |
| isolation and characterisation of a 13.8-kda bacteriolytic enzyme from house dust mite extracts: homology with prokaryotic proteins suggests that the enzyme could be bacterially derived. | bacteriolytic activity was detected in extracts of whole mite and spent growth medium (sgm) from the clinically important dermatophagoides pteronyssinus and dermatophagoides farinae mites and was most abundant in whole mite extract. gram-positive organisms micrococcus lysodeikticus, bacillus megaterium and listeria monocytogenes were preferentially lysed and the lytic activity was enhanced by thiols, destroyed by mite proteases, inhibited by hgcl2 and high concentrations of nacl but was resistan ... | 2002 | 12052562 |
| enhanced antimicrobial effects of combination of lactate and diacetate on listeria monocytogenes and salmonella spp. in beef bologna. | the antimicrobial activities of salts of organic acids such as lactate and acetate are well documented, but there is limited information on their effect when used in combination. we previously reported enhanced inhibition of listeria monocvtogenes and salmonella enterica serovar enteritidis in sterile comminuted beef at 5 and 10 degrees c by combinations of sodium lactate (sl) (2.5%) and sodium diacetate (sda) (0.2%). the present study was undertaken to evaluate the inhibitory effect of these sa ... | 2002 | 12051475 |
| [prosthetic hip joint infection caused by listeria monocytogenes]. | the authors report an unusual case of prosthetic hip joint infection caused by listeria monocytogenes. the patient, an 87-year-old lady who had undergone a right total hip replacement 10 years previously, presented with pain and restriction of hip motion three weeks after an episode of abdominal pain. aspiration of the joint yielded a dark fluid, from which listeria monocytogenes type 4-b was isolated. blood cultures remained negative. after prolonged antibiotic therapy, symptoms gradually resol ... | 2002 | 12051008 |
| [cervicovaginal infection as a risk factor for premature labor]. | to identify the possible association between cervicovaginal infections (cvi) and preterm delivery. | 2002 | 12050955 |
| ecology of listeria monocytogenes in the environment of raw poultry meat and raw pork meat processing plants. | the zoonotic listeria monocytogenes is mainly transmitted to humans by the food-borne route. this bacterium was often found in the environment of food processing plants. therefore the aims of this study were (i) the identification of environmental factors associated with l. monocytogenes contamination on working and non-working surfaces in poultry or pork processing plants and (ii) the understanding of its survival in such environments. the physicochemical risk profiles showed that a surface in ... | 2002 | 12044686 |
| [opportunistic bacteria detected in cultivated mussels]. | as many as 8 listeria monocytogenes strains, 12 pseudomonas aeruginosa strains and 5 staphylococcus aureus strains were isolated from mussels mytilus edulis, grown on special installations in the trinity bay of the gulf of peter the great, the sea of japan. the isolated cultures proved to be highly resistant to a number of antibiotics. many strains displayed dnaase and haemolytic activity. the cultures of l. monocytogenes, s. aureus and p. aeruginosa also had high lipase, protease and lecithinas ... | 2002 | 12043163 |
| dietary calcium phosphate promotes listeria monocytogenes colonization and translocation in rats fed diets containing corn oil but not milk fat. | most gram-positive bacteria are susceptible to the bactericidal action of fatty acids and bile acids. because dietary calcium phosphate (cap(i)) lowers the intestinal concentration of these antimicrobial agents, high cap(i) intake may enhance intestinal colonization of gram-positive pathogens and the subsequent pathogenesis. in this study, we tested this hypothesis in a rat model using listeria monocytogenes. rats were fed diets containing low (20 micromol/g diet) or high (160 micromol/g diet) a ... | 2002 | 12042445 |
| molecular characterization of the genes encoding dna gyrase and topoisomerase iv of listeria monocytogenes. | the genes encoding subunits a and b of dna gyrase and subunits c and e of topoisomerase iv of listeria monocytogenes, gyra, gyrb, parc and pare, respectively, were cloned and sequenced. compared with the sequences of quinolone-susceptible bacteria, such as escherichia coli and bacillus subtilis, the quinolone resistance-determining region (qrdr) of dna gyrase subunit a was altered; the deduced amino acid sequences revealed the substitutions ser-84-->thr and asp/glu-88-->phe, two amino acid varia ... | 2002 | 12039883 |
| microtiter plate assay for assessment of listeria monocytogenes biofilm formation. | listeria monocytogenes has the ability to form biofilms on food-processing surfaces, potentially leading to food product contamination. the objective of this research was to standardize a polyvinyl chloride (pvc) microtiter plate assay to compare the ability of l. monocytogenes strains to form biofilms. a total of 31 coded l. monocytogenes strains were grown in defined medium (modified welshimer's broth) at 32 degrees c for 20 and 40 h in pvc microtiter plate wells. biofilm formation was indirec ... | 2002 | 12039754 |
| ability of the listeria monocytogenes strain scott a to cause systemic infection in mice infected by the intragastric route. | listeriosis is an important food-borne disease that causes high rates of morbidity and mortality. for reasons that are not clear, most large outbreaks of human listeriosis involve listeria monocytogenes serotype 4b. relatively little is known about the pathogenesis of listeriosis following gastrointestinal exposure to food-borne disease isolates of l. monocytogenes. in the present study, we investigated the pathogenesis of systemic infection by the food-borne isolate scott a in an intragastric ( ... | 2002 | 12039747 |
| combined ribotyping and random multiprimer dna analysis to probe the population structure of listeria monocytogenes. | to improve our understanding of the genetic links between strains originating from food and strains responsible for human diseases, we studied the genetic diversity and population structure of 130 epidemiologically unrelated listeria monocytogenes strains. strains were isolated from different sources and ecosystems in which the bacterium is commonly found. we used rrna gene restriction fragment length polymorphism analysis with two endonucleases and random multiprimer dna analysis with seven oli ... | 2002 | 12039742 |
| variation of branched-chain fatty acids marks the normal physiological range for growth in listeria monocytogenes. | the fatty acid composition of listeria monocytogenes scott a was determined by close-interval sampling over the entire biokinetic temperature range. there was a high degree of variation in the percentage of branched-chain fatty acids at any given temperature. the percentage of branched c17 components increased with growth temperature in a linear manner. however, the percentages of iso-c15:0 (i15:0) and anteiso-c15:0 (a15:0) were well described by third-order and second-order polynomial curves, r ... | 2002 | 12039736 |
| identification of opuc as a chill-activated and osmotically activated carnitine transporter in listeria monocytogenes. | the food-borne pathogen listeria monocytogenes is notable for its ability to grow under osmotic stress and at low temperatures. it is known to accumulate the compatible solutes glycine betaine and carnitine from the medium in response to osmotic or chill stress, and this accumulation confers tolerance to these stresses. two permeases that transport glycine betaine have been identified, both of which are activated by hyperosmotic stress and one of which is activated by low temperature. an osmotic ... | 2002 | 12039715 |
| application of nontraditional meat starter cultures in production of hungarian salami. | listeria monocytogenes and escherichia coli o111 have been implicated in several outbreaks of food-borne disease linked to smallgoods products. traditional meat starter cultures, containing a mixture of lactic acid bacteria (lab) and staphylococci, are used to maintain safety and sensory properties of hungarian salami. the present study investigated if nontraditional meat starter (ntms) cultures can be used for improving the safety of hungarian salami. salami batter was inoculated with list. mon ... | 2002 | 12038580 |
| genetic homogeneity among listeria monocytogenes strains from infected patients and meat products from two geographic locations determined by phenotyping, ribotyping and pcr analysis of virulence genes. | thirty listeria monocytogenes isolates from human patients and foods originated from two different geographic locations without any epidemiological relations were analyzed for their genotypic and phenotypic virulence gene expressions and genetic relatedness. all strains contained virulence genes, inla, inlb, acta, hlya, plca and plcb, with expected product size in pcr assay except for the acta gene. some strains produced acta gene product of 268 and others 385 bp. phenotypically, all were hemoly ... | 2002 | 12038565 |