Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| genomic analysis of a region encompassing qrfs1 and qrfs2: genes that underlie soybean resistance to sudden death syndrome. | candidate genes were identified for two loci, qrfs2 providing resistance to the leaf scorch called soybean (glycine max (l.) merr.) sudden death syndrome (sds) and qrfs1 providing resistance to root infection by the causal pathogen fusarium solani f.sp. glycines. the 7.5 +/- 0.5 cm region of chromosome 18 (linkage group g) was shown to encompass a cluster of resistance loci using recombination events from 4 near-isogenic line populations and 9 dna markers. the dna markers anchored 9 physical map ... | 2005 | 15729404 |
| a generic system for the escherichia coli cell-surface display of lipolytic enzymes. | esta is an outer membrane-anchored esterase from pseudomonas aeruginosa. an inactive esta variant was used as an anchoring motif for the escherichia coli cell-surface display of lipolytic enzymes. flow cytometry analysis and measurement of lipase activity revealed that bacillus subtilis lipase lipa, fusarium solani pisi cutinase and one of the largest lipases presently known, namely serratia marcescens lipase were all efficiently exported by the esta autotransporter and also retained their lipol ... | 2005 | 15710409 |
| concordant evolution of trichothecene 3-o-acetyltransferase and an rdna species phylogeny of trichothecene-producing and non-producing fusaria and other ascomycetous fungi. | the cereal pathogen fusarium graminearum species complex (e.g. fusarium asiaticum, previously referred to as f. graminearum lineage 6) produces the mycotoxin trichothecene in infected grains. the fungus has a gene for self-defence, tri101, which is responsible for 3-o-acetylation of the trichothecene skeleton in the biosynthetic pathway. recently, trichothecene non-producers fusarium oxysporum and fusarium fujikuroi (teleomorph gibberella fujikuroi) were shown to have both functional (tri201) an ... | 2005 | 15699200 |
| recurrent colonization of successively implanted tracheoesophageal vocal prostheses by a member of the fusarium solani species complex. | tracheoesophageal vocal prostheses (tvp) in laryngectomized patients commonly deteriorate due to overgrowth by yeasts, particularly candida species. we describe the first case of colonization of such devices by a member of the fusarium solani species complex in a patient with a history of glottal carcinoma. three isolates, from three prostheses, were found morphologically consistent with the traditional picture of f. solani. ribosomal sequence analysis showed that the isolates belonged to a dist ... | 2005 | 15695678 |
| antimicrobial and insecticidal protein isolated from seeds of clitoria ternatea, a tropical forage legume. | the tropical forage legume clitoria ternatea (l.) has important agronomic traits such as adaptation to a wide range of soil conditions and resistance to drought. it is resistant to a number of pathogens and pests. these important traits gave us reasons to look more closely at the plant. a highly basic small protein was purified from seeds of c. ternatea to homogeneity by using ultrafiltration with centricon-3 membrane tubes and preparative granulated-bed isoelectric focusing (ief). a single prot ... | 2004 | 15694280 |
| chemical composition and antifungal activity of essential oils from different tissues of japanese cedar (cryptomeria japonica). | in this study antifungal activities of essential oils from different tissues of japanese cedar (cryptomeria japonica d. don) against four wood decay fungi and six tree pathogenic fungi were investigated. in addition, the yields of essential oils obtained by water distillation were compared and their constituents determined by gc-ms analyses. the yield of essential oils from four tissues of japanese cedar is in the decreasing order of leaf (27.38 ml/kg) > bark (6.31 ml/kg) > heartwood (3.80 ml/kg ... | 2005 | 15686410 |
| potential ecotoxicological implication of methyl tert-butyl ether (mtbe) spills in the environment. | streptomyceticidal activity of methyl tert-butyl ether (mtbe) elucidated for the first time. adverse effect of mtbe, the gasoline additive, against 11 soil inhabitant streptomyces spp. isolates was investigated. mtbe, an octane enhancer is added to gasoline to reduce atmospheric concentrations of carbon monoxide and ozone. it contaminates soil and groundwater by fuel leaks and spills. streptomyces spp. are of the major contributors to the biological buffering of soils by exerting beneficial and ... | 2004 | 15673212 |
| first report of a novel plant lysozyme with both antifungal and antibacterial activities. | a novel lysozyme exhibiting antifungal activity and with a molecular mass of 14.4kda in sds-polyacrylamide gel electrophoresis was isolated from mung bean (phaseolus mungo) seeds using a procedure that involved aqueous extraction, ammonium sulfate precipitation, ion exchange chromatography on cm-sephadex, and high-performance liquid chromatography on poros hs-20. its n-terminal sequence was very different from that of hen egg white lysozyme. its pi was estimated to be above 9.7. the specific act ... | 2005 | 15649419 |
| isatin-derived antibacterial and antifungal compounds and their transition metal complexes. | a series of isatins incorporating thiazole, thiadiazole, benzothiazole and p-toluene sulfonyl hydrazide moieties, along with their cobalt(ii), copper(ii), nickel(ii) and zinc(ii) metal complexes have been synthesized and characterized by elemental analyses, molar conductances, magnetic moments, ir, nmr and electronic spectral data. these compounds have been screened for antibacterial activity against escherichia coli, bacillus subtillis, shigella flexneri, staphylococcus aureus, pseudomonas aeru ... | 2004 | 15648656 |
| effect of voriconazole on a corneal abscess caused by fusarium. | to describe a case of corneal abscess caused by fusarium solani that did not respond to common antifungal agents. | 2004 | 15606478 |
| fusarium solani endocarditis successfully treated with liposomal amphotericin b and voriconazole. | fungal infections caused by fusarium in the immunocompromised host are highly resistant to all antifungal agents. fusarium endocarditis is a rare and usually fatal disease. we report an immunocompromised child who survived fusarium solani endocarditis despite the in vitro resistance of the organism to all available antifungal agents. | 2004 | 15545866 |
| polycyclic aromatic hydrocarbons storage by fusarium solani in intracellular lipid vesicles. | accumulation and elimination of polycyclic aromatic hydrocarbons (pahs) were studied in the fungus fusarium solani. when the fungus was grown on a synthetic medium containing benzo[a]pyrene, hyphae of f. solani contained numerous lipid vesicles which could be stained by the lipid-specific dyes: sudan iii and rhodamine b. the fluorescence produced by rhodamine b and pah benzo[a]pyrene were at the same locations in the fungal hyphae, indicating that f. solani stored pah in pre-existing lipid vesic ... | 2005 | 15519459 |
| in vitro susceptibility testing of amorolfine in pathogenic fungi isolated from dermatomycosis patients in china. | the antifungal susceptibility of isolates from chinese dermatomycosis patients to amorolfine was investigated following national committee for clinical laboratory standards (nccls) protocols. in total, 383 isolates were tested, including 132 strains from tinea pedis, 148 strains from tinea corporis/cruris, and 103 strains from onychomycosis. the minimum inhibitory concentration (mic) of amorolfine against dermatophytes ranged from 0.01 to 0.08 microg ml(-1). the mic(50) and mic(90) of amorolfine ... | 2004 | 15504124 |
| chemical interactions of brachiaria plantaginea with commelina bengalensis and acanthospermum hispidum in soybean cropping systems. | previous results obtained in soybean-wheat rotations under no-tillage conditions showed reductions in the seedbank of the weed species commelina benghalensis, but no alteration in the seedbank of acanthospermum hispidum in areas infested with brachiaria plantaginea. analyses of the soluble fraction of b. plantaginea indicated the predominance of aconitic acid (aa) among the aliphatic acids and ferulic acid (fa) among the phenolic acids. laboratory bioassays using c. benghalensis and a. hispidum ... | 2004 | 15503532 |
| effectiveness of contact lens disinfectants after lens storage. | to determine the effectiveness of multipurpose contact lens solutions after incubation with lenses for one week. | 2004 | 15499238 |
| genotyping of 44 isolates of fusarium solani, the main agent of fungal keratitis in brazil. | in a retrospective study performed over 6 years in brazil, fusarium solani was found to be the most common species causing mycotic keratitis. the genetic diversity of 44 isolates from 39 patients was assessed by enterobacterial repetitive intergenic consensus pcr (eric-pcr) and pcr restriction fragment length polymorphism (pcr-rflp) fingerprinting. eric-pcr was more discriminatory than pcr-rflp for differentiating the strains. by combining of the results of both techniques, we identified 40 geno ... | 2004 | 15472299 |
| production in vitro of toxic macromolecules by strains of beauveria bassiana, and purification of a chitosanase-like protein secreted by a melanizing isolate. | the production of macromolecular insecticidal toxins in adamek's medium by two selected strains of beauveria bassiana was investigated. filtrates and dialysates of the melanizing strain 618 were toxic when injected into the lepidopteran insect galleria mellonella. separation by deae chromatography revealed that peaks eluted respectively with 100 and 200 mm nacl (p 100 and p 200) had an insecticidal activity and induced cuticular blackening. a hydrophilic protein, bclp, which causes the formation ... | 2004 | 15450862 |
| antibacterial and antifugal mono- and di-substituted symmetrical and unsymmetrical triazine-derived schiff-bases and their transition metal complexes. | a new series of antibacterial and antifungal triazine-derived mono- and di-substituted (symmetrical and unsymmetrical) schiff-bases and their cobalt(ii), copper(ii), nickel(ii) and zinc(ii) metal complexes have been synthesized and characterized by their elemental analyses, molar conductances, magnetic moments and ir and electronic spectral measurements. ir spectra indicated the ligands to act as tridentate towards divalent metal ions via a trazine-n, the azomethine-n and, indole-nh and deproton ... | 2004 | 15449731 |
| a non-specific lipid transfer protein with antifungal and antibacterial activities from the mung bean. | a non-specific lipid transfer peptide (nsltp) with antimicrobial activity was isolated from the mung bean (phaseolus mungo) seeds. the procedure entailed aqueous extraction, ion exchange chromatography on cm-sephadex and high performance liquid chromatography (hplc) on poros-hs-20. the peptide exhibited a molecular mass of 9.03 kda in mass spectrometry. it exerted antifungal action toward fusarium solani, fusarium oxysporum, pythium aphanidermatum and sclerotium rolfsii, and antibacterial action ... | 2004 | 15350690 |
| intracranial fusariosis: a novel cause of fungal meningoencephalitis in a dog. | the fusarium species are a group of saprophytic fungal organisms that are occasionally the cause of opportunistic infections in humans and animals. central nervous system disease associated with a fusarium species is most commonly described in horse, resulting in equine leukoencephalomalacia. this report describes a 2-year-old, spayed, female german shepherd dog with meningoencephalitis secondary to infection with fusarium spp. meningoencephalitis in dogs secondary to a species of fusarium has n ... | 2004 | 15347824 |
| isoflavonoid accumulation in soybean hairy roots upon treatment with fusarium solani. | hairy roots were initiated from two soybean [glycine max (l.) merr.] genotypes with different susceptibility (susceptible 'spencer' and partially resistant 'pi567.374') to the disease sudden death syndrome (sds) caused by the soil-borne fungal pathogen fusarium solani f. sp. glycines (fsg) to study the role of isoflavonoids in the plant response to fsg infection. hairy root cultures obtained by transformation with agrobacterium rhizogenes allows normal root growth that can be visually monitored. ... | 2004 | 15331097 |
| [assessment of airborne mycoflora in critical areas of the principal hospital of cumaná, state of sucre, venezuela]. | the study of the nosocomial infections of fungic origin has attained importance in the last years, due to the rise in the number of patients that are inmunocompromised and susceptible to suffer this kind of infection. the objective of the present study was to evaluate the frequency of filamentous fungi and yeast, present in the environment of the intensive care unit, operating and newborn children rooms of the hospital universitario "antonio patricio de alcalá" (huapa) from the city of cumaná. s ... | 2004 | 15211981 |
| antifungal cobalt(ii), copper(ii), nickel(ii) and zinc(ii) complexes of furanyl-,thiophenyl-, pyrrolyl-, salicylyl- and pyridyl-derived cephalexins. | some novel cephalexin-derived furanyl, thiophenyl, pyrrolyl, salicylyl and pyridyl schiff's bases and their cobalt (ii), copper (ii), nickel (ii) and zinc (ii) complexes have been synthesized and studied for their antifungal properties against trichophyton longifusus, candida albicans, aspergillus flavus, microsporum canis, fusarium solani and candida glaberata. the presence of metal ions in the investigated schiff's base complexes reported here lead to significant antifungal activity, whereas t ... | 2004 | 15202498 |
| immunosuppression affects the severity of experimental fusarium solani keratitis. | we have established a mouse model of corneal fusariosis that permits the evaluation of fungal infection and pathogenesis. corneas of immunocompetent and cyclophosphamide-treated adult balb/c mice were topically inoculated with fusarium solani after corneal scarification. eyes were scored for corneal involvement daily for 8 days and at 2 weeks after infection. eyes were enucleated at various time points for quantitative fungal recovery and histopathological examination. an inoculum-dose response ... | 2004 | 15195260 |
| onychomycosis caused by fusarium solani and fusarium oxysporum in são paulo, brazil. | fusarium species are common soil saprophytes and plant pathogens that have been frequently reported as etiologic agents of opportunistic infections in humans. we report eight cases of onychomycosis caused by fusarium solani (4) and fusarium oxysporum (4) in são paulo, brazil. these species were isolated from toenails in all cases. the infections were initially considered to be caused by dermatophytes. the clinical appearance of the affected toenails was leukonychia or distal subungual hyperkerat ... | 2004 | 15180157 |
| vesicles as initial skin manifestation of disseminated fusariosis after non-myeloablative stem cell transplantation. | a 52-year-old man underwent non-myeloablative stem cell transplantation from his hla identical sister for the treatment of mantle cell lymphoma. on day 0, he developed a high-grade fever, watery diarrhea and vesicles scattered on the skin. well experienced dermatologists diagnosed these lesions as vzv reactivation. high dose antiviral agents were ineffective, and fusarium solani was cultured from his stool and sputum. systemic fusariosis progressed rapidly and he died of multiorgan failure on da ... | 2004 | 15160931 |
| effect of soil moisture on seed germination, plant growth and root rot severity of navy bean in fusarium solani infested soil. | the development of root rot and its severity were affected by numerous factors which through their interaction could distort results. in this study, a single factor, soil moisture was considered. using fusarium solani infested root rot soil we investigated the effect of three available soil moisture (asm) levels (i.e. 100%, 70% and 25%) on seed rot, germination, plant growth and root rot severity of navy bean in the greenhouse. percent germination differed strikingly averaging of 58.5, 89.5 and ... | 2003 | 15151296 |
| phenylpropanoids, phenylalanine ammonia lyase and peroxidases in elicitor-challenged cassava (manihot esculenta) suspension cells and leaves. | control of diseases in the key tropical staple, cassava, is dependent on resistant genotypes, but the innate mechanisms are unknown. the aim was to study phenylpropanoids and associated enzymes as possible defence components. | 2004 | 15145789 |
| biodegradation of aliphatic and aromatic hydrocarbons by natural soil microflora and pure cultures of imperfect and lignolitic fungi. | the biodegradation of aliphatic and aromatic hydrocarbons by natural soil microflora and seven fungi species, including imperfect strains and higher level lignolitic species, is compared in a 90-day laboratory experiment using a natural, not-fertilized soil contaminated with 10% crude oil. the natural microbial soil assemblage isolated from an urban forest area was unable to significantly degrade crude oil, whereas pure fungi cultures effectively reduced the residues by 26-35% in 90 days. normal ... | 1996 | 15093496 |
| mistletoe viscotoxins induce membrane permeabilization and spore death in phytopathogenic fungi. | viscotoxins (vts) are basic peptides expressed in mistletoe leaves, seeds and stems which have been shown to be cytotoxic to mammalian cells. the aim of this study was to analyse whether vts were able to control and/or inhibit the growth of phytopathogenic fungi to obtain a clue to their biological function. incubation of two vt isoforms, vta(3) and vtb, at a final concentration of 10 micro m resulted in a complete blockage of the germination of spores from three different pathogenic fungi. it w ... | 2004 | 15086811 |
| site-specific and reversible anchoring of active proteins onto cellulose using a cellulosome-like complex. | protein engineering strategies facilitating controlled and spontaneous assembly of macromolecular complexes are of great interest for the design of artificial multi-enzyme systems of pre-defined composition. here we have combined affinity proteins from different sources to achieve specific and reversible anchoring of affinity domain-tagged reporter proteins to a cellulose-anchored fusion protein. the design principle mimics the architecture of macromolecular cellulosome complexes produced by som ... | 2004 | 15066765 |
| recombinant saccharomyces cerevisiae strain triggers acetate production to fuel biosynthetic pathways. | although the metabolism and physiology of the growth of yeast strains has been extensively studied, many questions remain unanswered when the induced production of a recombinant protein is concerned. this work addresses the production of a fusarium solani pisi cutinase by a recombinant saccharomyces cerevisiae strain induced through the use of a galactose promoter. it was observed that whenever the strain needed to activate biosynthetic pathways, either for cutinase synthesis, or for the synthes ... | 2004 | 15063624 |
| purification and characterization of a major cross-reactive allergen from epicoccum purpurascens. | epicoccum purpurascens (formerly nigrum) (ep), is a ubiquitous saprophytic mould found both indoors and outdoors. several studies have reported sensitization to ep in 5-7% of different populations worldwide. the diagnosis of mould allergy requires a standardized fungal extract that contains all its important allergenic proteins. the crude allergen extract from ep was standardized earlier, however none of its allergens have been purified. | 2004 | 14976389 |
| fusarium solani fungemia in a patient with acute lymphoblastic leukemia. | a patient with all on anticancer chemotherapy developed fever which was later attributed to be due to fusarium fungemia. the details of the case & a review of literature follows. | 2001 | 14758881 |
| effect of immobilization support, water activity, and enzyme ionization state on cutinase activity and enantioselectivity in organic media. | we studied the reaction between vinyl butyrate and 2-phenyl-1-propanol in acetonitrile catalyzed by fusarium solani pisi cutinase immobilized on zeolites naa and nay and on accurel pa-6. the choice of 2-phenyl-1-propanol was based on modeling studies that suggested moderate cutinase enantioselectivity towards this substrate. with all the supports, initial rates of transesterification were higher at a water activity (a(w)) of 0.2 than at a(w) = 0.7, and the reverse was true for initial rates of h ... | 2004 | 14755562 |
| preliminary evidence of the role of hydrogen peroxide in the degradation of benzo[a]pyrene by a non-white rot fungus fusarium solani. | in order to study the enzymatic mechanisms involved in the successive steps of bap degradation by a deuteromycete fungus fusarium solani, we developed an indirect approach by using inhibitors of enzymes. we used either specific inhibitors of peroxidases (i.e. salicylhydroxamic acid) and of cytochrome p-450 (i.e. piperonyl butoxyde) or inhibitors of both enzymes (i.e. potassium cyanide). surprisingly, no expected decrease of bap degradation was observed with most inhibitors tested. on the contrar ... | 2004 | 14749063 |
| interactions of human phagocytes with moulds fusarium spp. and verticillium nigrescens possessing different pathogenicity. | fusarium spp. are emerging as important causes of invasive fungal infections. they tend to have decreased susceptibility to antifungal agents, making host defences very important. the ability of human phagocytes to cause damage to hyphae of fusarium solani, f. oxysporum and verticillium nigrescens, a mould with very low pathogenicity, was assessed using the 2,3-bis[2-methoxy-4-nitro-5-sulphophenyl]2h-tetrazolium-5-carboxanilide (xtt) metabolic assay. the oxidative burst, evidenced as superoxide ... | 2003 | 14725324 |
| fusarium osteomyelitis of the foot in a patient with diabetes mellitus. | diabetic foot osteomyelitis is among the most common and serious complications in patients with diabetes mellitus. it is often a polymicrobial infection. we report the first case of foot osteomyelitis in a diabetic patient caused by fusarium solani. | 2003 | 14723375 |
| isolation and characterization of a lectin from annona muricata seeds. | a lectin with a high affinity for glucose/mannose was isolated from annona muricata seeds (annonaceae) by gel filtration chromatography on sephacryl s-200, ion exchange chromatography on a deae sp-5 pw column, and molecular exclusion on a protein pak glass 300 sw column. sodium dodecyl sulfate polyacrylamide gel electrophoresis (page) yielded two protein bands of approximately 14 kda and 22 kda. however, only one band was seen in native page. the mr of the lectin estimated by fast-performance li ... | 2003 | 14714732 |
| fusarium solani infection complicated by tuberculous omarthritis. | 2003 | 14673299 | |
| two unrelated double-stranded rna molecule patterns in gremmeniella abietina type a code for putative viruses of the families totiviridae and partitiviridae. | two double stranded (ds) rna molecule patterns, probably of viral origin, were sequenced from gremmeniella abietina var. abietina type a. the genome of gremmeniella abietina rna virus l1 (garv-l1) from isolate hr2 was 5133 bp and contained two open reading frames (orfs). the 5'-proximal orf coded for a putative coat protein (cp) and the 3'-proximal orf encoded putative rna-dependent rna polymerase (rdrp). garv-l1 had sequence similarities with a previously described totivirus (helminthosporium v ... | 2003 | 14648287 |
| successful voriconazole therapy of disseminated fusarium solani in the brain of a neutropenic cancer patient. | 2003 | 14581897 | |
| [phytotoxic activity of chernozem saprophytic micromycetes: specificity, sorption and stability of phytotoxins in soil]. | micromycetes of the complex of typical chernozem saprotrophic fungi released phytotoxic metabolites into medium. the metabolites displayed their phytotoxic activities directly in soil. evaluation of the toxicities, range of biological effects activities, and stabilities of phytotoxins in soil and the rates of their biodegradation allowed the species that can serve as indicators of chernozem microbial toxicosis to be selected, namely, aspergillus clavatus, fusarium solani, talaromyces flavus, pen ... | 2003 | 14520964 |
| improvement of phenylethanoid glycosides production by a fungal elicitor in cell suspension culture of cistanche deserticola. | when, on the 15th day of growth, an elicitor from fusarium solani was added at 40 mg l(-1) to cistanche deserticola cell suspension cultures, the contents of echinacoside, acteoside and total phenylethanoid glycosides (pegs) in cultured cells all increased over the next 27 d by over 100% to 15 mg g(-1) dry wt, 9 mg g(-1) dry wt and 57 mg g(-1) dry wt, respectively. the final biomass (1.3 mg dry wt ml(-1)) was not affected. | 2003 | 14514046 |
| pentamidine is active in vitro against fusarium species. | fusariosis is an emerging opportunistic mycosis against which currently used antifungals have limited activity. here, we investigated the in vitro activities of pentamidine (pnt) against 10 clinical isolates of fusarium species (five fusarium solani isolates and five non-f. solani isolates) by using the national committee for clinical laboratory standards microdilution method in three different media (rpmi, rpmi-2, and a yeast nitrogen base medium), disk diffusion testing, and viability dye stai ... | 2003 | 14506038 |
| vigorous mold growth in soils after addition of water-insoluble fatty substances. | various water-insoluble fatty compounds, when added to soil in finely divided form, will support as high-caloric nutrients a visible, vigorous growth of the molds, fusarium solani mart., f. diversisporum sherb., and f. equiseti. n-paraffins and olefins are most effective, because the effect of additives is reduced to the extent that oxygen atoms are introduced into the molecule. n-fatty alcohols support growth in soil almost as well as the paraffins; however, growth is reduced when branched-chai ... | 1965 | 14325872 |
| spore germination and carbon metabolism in fusarium solani. iv. metabolism of ethanol and acetate. | cochrane, vincent w. (wesleyan university, middletown, conn.), jean c. cochrane, james m. vogel, and roswell s. coles, jr. spore germination and carbon metabolism in fusarium solani. iv. metabolism of ethanol and acetate. j. bacteriol. 86:312-319. 1963.-the aerobic metabolism of acetate and ethanol by ungerminated spores of fusarium solani f. phaseoli was accompanied by oxidative assimilation, respectively, of 50 and 75% of the substrate carbon. manometric and isotope distribution studies sugges ... | 1963 | 14058958 |
| microbiological oxidation of progesterone by fusarium solani. | 1955 | 13289284 | |
| soil bacteria and chlamydospore formation in fusarium solani. | 1952 | 13013244 | |
| monacrosporium janus sp. nov., a new nematode-trapping hyphomycete parasitizing sclerotia and hyphae of sclerotinia sclerotiorum. | during an investigation of mycoparasitic fungi on sclerotia of sclerotinia sclerotiorum in china, a new fungal species was consistently encountered and isolated from natural soils taken from soybean fields of shandong and jiangsu provinces. the fungus is featured by its sphaeroid conidia with 1-2 transverse septa, but mostly (>65%) with only one septum at the base. it resembles monacrosporium indicum, m. sphaeroides and m. sinense, but can be distinguished from the first two species by lack of b ... | 2003 | 12967217 |
| experimental murine hyalohyphomycosis with soil-derived isolates of fusarium solani. | two strains of soil-borne fusarium solani, both characterized for their ability to produce cyclosporin a and c, were examined for their pathogenicity in severe combined immunodeficiency (scid) and balb/c male mice. intravenous (i.v.) infections with f. solani conidia were performed. no mortality was observed after infection with 0.3-1.6 x 10(7) cfu per mouse in scid and balb/c mice. when mice were infected with 0.8-1.5 x 10(6) cfu per mouse and 2 days later with 1.2-1.9 x 10(6) cfu per mouse, 28 ... | 2003 | 12964716 |
| fluorescence of the single tryptophan of cutinase: temperature and ph effect on protein conformation and dynamics. | the cutinase from fusarium solani pisi is an enzyme with a single l-tryptophan (trp) involved in a hydrogen bond with an alanine (ala) residue and located close to a cystine formed by a disulfide bridge between two cysteine (cys) residues. the cys strongly quenches the fluorescence of trp by both static and dynamic quenching mechanisms. the trp fluorescence intensity increases by about fourfold on protein melting because of the disruption of the ala-trp hydrogen bond that releases the trp from t ... | 2003 | 12929743 |
| [disseminated fusarium solani infection in patients undergoing hematopoietic stem cell transplantation]. | a patient with a hematological malignancy is one example of a type of immunocompromised host, and critical opportunistic infections such as mycosis are not rare during medical treatment for such malignancy. candidiasis and aspergillosis are typical mycoses and their importance has been recognized widely and great progress attained in their prevention and medical treatment. however, allogenic hematopoietic stem cell transplantation (allo-hsct) to treat hematological malignancy has spread, and the ... | 2003 | 12913807 |
| atomic force microscopy study of the interaction of fusarium solani pisi cutinase with lipid surfaces. | we present an atomic force microscopy (afm) study of a supported triacylglyceride multilayer phase and its interaction with lipolytic enzyme cutinase from fusarium solani pisi. the multilayer triacylglyceride phase of coconut oil showed a rippled surface structure in the afm images. upon enzymatic degradation of the triacylglyceride phase, the ripple structure vanished rapidly. the apparent catalytic rate constants could be estimated based on the afm image information. interestingly, in one samp ... | 2002 | 12908312 |
| non-pathogenic fusarium solani represses the biosynthesis of nematicidal compounds in vitro and reduces the biocontrol of meloidogyne javanica by pseudomonas fluorescens in tomato. | the aim of the present investigation was to determine the influence of various fusarium solani strains on the production of nematicidal agent(s) in vitro and biocontrol of meloidogyne javanica in tomato by pseudomonas fluorescens strains cha0 and cha0/pme3424. | 2003 | 12859651 |
| synthesis and antimicrobial activity of n-(substituted)-n'-(2,3-dihydro-2-oxido-5-benzoyl-1h-1,3,2-benzodiazaphosphol-2-yl) ureas. | n-(substituted)-n'-(2,3-dihydro-5-benzoyl-2-oxido-1h-1,3,2-benzodiazaphosphol-2-yl) ureas were synthesized by reacting 3,4-diaminobenzophenone (4) with different chlorides of carbamidophosphoric acids (3) in the presence of triethylamine at 40-45 degrees c. their 1h-, 13c- and 31p-nmr spectral data are discussed. the title compounds were screened for antifungal and antibacterial activity against the fungi aspergillus niger and fusarium solani and bacteria escherichia coli and staphylococcus aure ... | 2003 | 12843597 |
| [post traumatic subcutaneous mycosis due to fusarium solani]. | we report the case of a subcutaneous hyalohyphomycosis of a 24-year-old man, a rural worker with an ulcerative lesion in the right leg of approximately one year duration. it was caused by traumatic implantation of a yerba mate branch. the diagnosis was made by direct microscopic examination with 20% potassium hydroxide (koh) and revealed several septate hyaline hyphae. it was confirmed by culture of several samples where fusarium solani was isolated. the patient received local and systemic antif ... | 2003 | 12825979 |
| a binuclear zinc transcription factor binds the host isoflavonoid-responsive element in a fungal cytochrome p450 gene responsible for detoxification. | the pda1 gene of the filamentous fungus nectria haematococca mpvi (anamorph: fusarium solani) encodes pisatin demethylase, a cytochrome p450. pisatin is a fungistatic isoflavonoid produced by garden pea (pisum sativum), a host for this fungus. pisatin demethylase detoxifies pisatin and functions as a virulence factor for this fungus. pisatin induces pda1 expression both in cultured mycelia as well as during pathogenesis on pea. the regulatory element within pda1 that provides pisatin-responsive ... | 2003 | 12823815 |
| advanced fusarium keratitis progressing to endophthalmitis. | to review the clinical course, treatment, and visual outcomes of keratitis with endophthalmitis caused by the filamentary fungus fusarium. | 2003 | 12792470 |
| improving the mouse model for studying the efficacy of voriconazole. | outbred icr mice were rendered neutropenic, infected intravenously with fusarium solani and treated orally with voriconazole. when given alone, voriconazole was not protective up to 40 mg/kg/day. when grapefruit juice was administered before infection, mice were protected by voriconazole. the mechanism may be inhibition of gut mucosal cytochrome enzymes that rapidly degrade voriconazole in the mouse. these murine studies support expansion of voriconazole therapy in other highly resistant systemi ... | 2003 | 12746374 |
| volatile metabolites from salvia fruticosa as antifungal agents in soilborne pathogens. | the volatile metabolites of salvia fruticosa plants, growing wild in 15 localities scattered across greece, were analyzed by means of gc and gc-ms. the essential oil content ranged from 0.69 to 4.68%, and the results of the analyses showed a noticeable variation in the amounts of the five main components [1,8-cineole, alpha-thujone, beta-thujone, camphor, and (e)-caryophyllene]. the antifungal activities of the essential oils from two localities, belonging in two different groups of cluster and ... | 2003 | 12744657 |
| activity, conformation and dynamics of cutinase adsorbed on poly(methyl methacrylate) latex particles. | the adsorption of a recombinant cutinase from fusarium solani pisi onto the surface of 100 nm diameter poly(methyl methacrylate) (pmma) latex particles was evaluated. adsorption of cutinase is a fast process since more than 70% of protein molecules are adsorbed onto pmma at time zero of experiment, irrespective of the tested conditions. a langmuir-type model fitted both protein and enzyme activity isotherms at 25 degrees c. gamma(max) increased from 1.1 to 1.7 mg m(-2) and u(max) increased from ... | 2003 | 12730007 |
| activity of the amidoamine myristamidopropyl dimethylamine against keratitis pathogens. | microbial keratitis accounts for up to 30% of blindness in some less developed societies. the development of a single broad-spectrum topical antimicrobial effective against bacteria, fungi and acanthamoeba would have a major impact on reducing the morbidity and simplifying the treatment of microbial keratitis. to this end, the activity of the amidoamine myristamidopropyl dimethylamine (mapd) was investigated against common causes of microbial keratitis. | 2003 | 12716783 |
| antifungal effects of different organic extracts from melia azedarach l. on phytopathogenic fungi and their isolated active components. | extracts from different parts of melia azedarach l. were studied as potential antifungal agents for selected phytopathogenic fungi. in a serial agar dilution method, hexanic and ethanolic extracts from fruit, seed kernels, and senescent leaves exhibited fungistatic activity against aspergillus flavus,diaporthe phaseolorum var. meridionales, fusarium oxysporum, fusarium solani, fusarium verticillioides, and sclerotinia sclerotiorum. both hexanic extract from senescent leaves and ethanolic extract ... | 2003 | 12696928 |
| interaction between root lesion nematode pratylenchus vulnus and two species of fusarium on growth and development of maple seedlings. | producing healthy seedlings and distributing them to far areas, is one of the most important factor for developing forests. because of easy planting, rapid growth and good wood quality, acer velutinum is the most useful species, among the softwood and hardwood trees. growth and development of the nurseries were effected by different pests and diseases, the nematodes are one of these agents. they are not actively surveyed in the forest nurseries in iran. on a survey of maple seedling in the fores ... | 2002 | 12696440 |
| mycetoma of the foot caused by fusarium solani: identification of the etiologic agent by dna sequencing. | we report a case of fusarium solani mycetoma of the foot that could not be diagnosed by culture, but was correctly identified after amplification and sequence analysis of fungal internal transcribed spacers 1 and 2 and 5.8s ribosomal dna regions. | 2003 | 12682194 |
| purification and characterization of a cross-reactive 45-kd major allergen of fusarium solani. | fusarium solani (fs) is an important source of fungal allergen. a 45-kd major allergen of fs showed reactivity with patients' sera sensitive to many fungi. | 2003 | 12660423 |
| towards a cost effective strategy for cutinase production by a recombinant saccharomyces cerevisiae: strain physiological aspects. | although the physiology and metabolism of the growth of yeast strains has been extensively studied, many questions remain unanswered where the induced production of a recombinant protein is concerned. this work addresses the production of a fusarium solani pisi cutinase by a recombinant saccharomyces cerevisiae strain induced through the use of a galactose promoter. the strain is able to metabolise the inducer, galactose, which is a much more expensive carbon source than glucose. both the transp ... | 2003 | 12658517 |
| a diffusible factor from arbuscular mycorrhizal fungi induces symbiosis-specific mtenod11 expression in roots of medicago truncatula. | using dual cultures of arbuscular mycorrhizal (am) fungi and medicago truncatula separated by a physical barrier, we demonstrate that hyphae from germinating spores produce a diffusible factor that is perceived by roots in the absence of direct physical contact. this am factor elicits expression of the nod factor-inducible gene mtenod11, visualized using a pmtenod11-gusa reporter. transgene induction occurs primarily in the root cortex, with expression stretching from the zone of root hair emerg ... | 2003 | 12644648 |
| tryptophan-tagged cutinase studied by steady state fluorescence for understanding of tag interactions in aqueous two-phase systems. | genetic engineering has been used to construct fusion proteins of fusarium solani pisi cutinase and tryptophan-based tags, expressed in saccharomyces cerevisiae, to increase the partitioning in aqueous two-phase systems. the separation systems were composed of thermoseparating polymers (random copolymers of ethylene oxide and propylene oxide, eopo) and detergents (c(12)eo(n)). in this study, the fluorescence behaviour of the peptide-tagged protein, free peptide tag and tryptophan was investigate ... | 2003 | 12637012 |
| direct quantification of fungal dna from soil substrate using real-time pcr. | detection and quantification of genomic dna from two ecologically different fungi, the plant pathogen fusarium solani f. sp. phaseoli and the arbuscular mycorrhizal fungus glomus intraradices, was achieved from soil substrate. specific primers targeting a 362-bp fragment from the ssu rrna gene region of g. intraradices and a 562-bp fragment from the f. solani f. sp. phaseoli translation elongation factor 1 alpha gene were used in real-time polymerase chain reaction (pcr) assays conjugated with t ... | 2003 | 12609725 |
| a pyramid of loci for partial resistance to fusarium solani f. sp. glycines maintains myo-inositol-1-phosphate synthase expression in soybean roots. | myo-inositol 1-phosphate synthase (mips; ec 5.5.1.4) converts glucose 6-phosphate to myo-inositol 1-phosphate in the presence of nad(+). it catalyzes the first step in the synthesis of myo-inositol and pinitol, and is a rate limiting step in the de novo biosynthesis of inositol in eukaryotes. therefore, mips is involved in biotic and abiotic stress via ca(2+) signalling. seedlings of four soybean genotypes were inoculated with fusarium solani f. sp. glycines, the causative agent of sudden death ... | 2002 | 12582889 |
| common loci underlie field resistance to soybean sudden death syndrome in forrest, pyramid, essex, and douglas. | soybean [ glycine max (l.) merr.] sudden death syndrome (sds) caused by fusarium solani f. sp. glycines results in severe yield losses. resistant cultivars offer the most-effective protection against yield losses but resistant cultivars such as 'forrest' and 'pyramid' vary in the nature of their response to sds. loci underlying sds resistance in 'essex' x forrest are well defined. our objectives were to identify and characterize loci and alleles that underlie field resistance to sd ... | 2002 | 12582700 |
| localized cutaneous hyalohyphomycosis caused by a fusarium species infection in a renal transplant patient. | fusariosis is a hyalohyphomycosis due to fusarium species that mainly occurs in immunocompromised hosts. the clinical spectrum of fusarium infection comprises localized and disseminated forms. a case of localized cutaneous fusariosis caused by fusarium solani in a renal transplant patient is described, and the skin manifestations of the disease are discussed. | 2003 | 12574313 |
| effects of codon usage versus putative 5'-mrna structure on the expression of fusarium solani cutinase in the escherichia coli cytoplasm. | matching the codon usage of recombinant genes to that of the expression host is a common strategy for increasing the expression of heterologous proteins in bacteria. however, while developing a cytoplasmic expression system for fusarium solani cutinase in escherichia coli, we found that altering codons to those preferred by e. coli led to significantly lower expression compared to the wild-type fungal gene, despite the presence of several rare e. coli codons in the fungal sequence. on the other ... | 2003 | 12509995 |
| the thermal stability of the fusarium solani pisi cutinase as a function of ph. | we have investigated the thermal stability of the fusarium solani pisi cutinase as a function of ph, in the range from ph 2-12. its highest enzymatic activity coincides with the ph-range at which it displays its highest thermal stability. the unfolding of the enzyme as a function of ph was investigated by microcalorimetry. the ratio between the calorimetric enthalpy (deltah(cal)) and the van't hoff enthalpy (deltah(v)) obtained, is far from unity, indicating that cutinase does not exhibit a simp ... | 2001 | 12488611 |
| physicochemical characterization and antimicrobial properties of rhamnolipids produced by pseudomonas aeruginosa 47t2 ncbim 40044. | pseudomonas aeruginosa 47t2, grown in submerged culture with waste frying oil as a carbon source, produced a mixture of rhamnolipids with surface activity. up to 11 rhamnolipid homologs (rha-rha-c(8)-c(10); rha-c(10)-c(8)/rha-c(8)-c(10);rha-rha-c(8)-c(12:1); rha-rha-c(10)-c(10); rha-rha-c(10)-c(12:1); rha-c(10)-c(10); rha-rha-c(10)-c(12)/rha-rha-c(12)-c(10); rha-c(10)-c(12:1)/rha-c(12:1)-c(10); rha-rha-c(12:1)-c(12); rha-rha-c(10)-c(14:1); rha-c(10)-c(12)/rha-c(12)-c(10)) were isolated from cult ... | 2003 | 12474254 |
| complete sequence of the genome of two dsrna viruses from discula destructiva. | complete nucleotide sequences were determined for the four dsrna segments present in isolate 247 of discula destructiva from south carolina. the largest dsrna (dsrna 1) was 1787 bp in length with a single open reading frame (orf) that coded for a putative rna-dependent rna polymerase (rdrp). the dsrna 2 was 1585 bp in length with a single orf that coded for a putative viral coat protein. both the dsrna 3 (1178 bp in length) and dsrna 4 (308 bp) contained single orfs. however, neither the nucleot ... | 2002 | 12457976 |
| partitioning of peptide-tagged proteins in aqueous two-phase systems using hydrophobically modified micelle-forming thermoseparating polymer. | genetic engineering has been used to construct hydrophobically modified fusion proteins of cutinase from fusarium solani pisi and tryptophan-containing peptides. the aim was to enhance the partitioning of the tagged protein in a novel aqueous two-phase system formed by only one water-soluble polymer. the system was based on a hydrophobically modified random copolymer of ethylene oxide (eo) and propylene oxide (po) units, hm-eopo, with myristyl groups (c(14)h(29)) at both ends. the hm-eopo polyme ... | 2002 | 12445475 |
| resistance locus pyramids alter transcript abundance in soybean roots inoculated with fusarium solani f.sp. glycines. | soybean sudden death syndrome (sds) is caused by fusarium solani f.sp. glycines (fsg). six quantitative trait loci (qtls), each conferring partial resistance to sds, have been discovered in an essex x forrest recombinant inbred line (ril) population, but their mode of action is not clear. this study aimed to identify genes (ests) whose mrna transcripts were altered in abundance in soybean roots following inoculation of fsg. roots of the soybean variety forrest (four resistance alleles) were inoc ... | 2002 | 12436262 |
| damping-off in conifer seedling nurseries in noshahr and kelardasht. | to study the damping-off of conifer seedlings, we have collected samples from the roots of conifer seedlings (pinus nigra, picea excelsa, abieces spp, cupressus arizonica, cupressus sempervirens) from nurseries in the south of iran (noshahr and kelardasht). after disinfecting the samples, we have used standard media like pda, ma and cla. the following fungi were identified: fusarium solani, fusarium oxysporum, fusarium sambucinum, clamydosporium, rhizoctonia solani, cylindrocarpon spp., alternar ... | 2001 | 12425024 |
| integration of production and aqueous two-phase systems extraction of extracellular fusarium solani pisi cutinase fusion proteins. | genetic engineering was integrated with the production and purification of fusarium solani pisi cutinases, in order to obtain the highest amount of enzyme activity units, after purification. an aqueous two-phase system (atps) of polyethylene glycol 3350, dipotassium phosphate and whole broth was used for the extraction of three extracellular cutinases expressed in saccharomyces cerevisiae. the production/extraction process was evaluated regarding cutinases secretion in the medium, partition beha ... | 2003 | 12413786 |
| optimal testing conditions for determining mics and minimum fungicidal concentrations of new and established antifungal agents for uncommon molds: nccls collaborative study. | this collaborative three-center study evaluated nccls m38-a document testing conditions and other testing conditions for the antifungal susceptibility testing of 35 isolates of aspergillus nidulans, a. terreus, bipolaris hawaiiensis, b. spicifera, cladophialophora bantiana, dactylaria constricta, fusarium solani, paecilomyces lilacinus, scedosporium prolificans, trichoderma longibrachiatum, and wangiella dermatitidis for itraconazole, three new triazoles (voriconazole, posaconazole, and ravucona ... | 2002 | 12354880 |
| bioactivation of the fungal phytotoxin 2,5-anhydro-d-glucitol by glycolytic enzymes is an essential component of its mechanism of action. | an isolate of fusarium solani, nrrl 18883, produces the natural phytotoxin 2,5-anhydro-d-glucitol (ahg). this fungal metabolite inhibited the growth of roots (150 of 1.6 mm), but it did not have any in vitro inhibitory activity. the mechanism of action of ahg requires enzymatic phosphorylation by plant glycolytic kinases to yield ahg-1,6-bisphosphate (ahg-1,6-bisp), an inhibitor of fru-1,6-bisp aldolase. ahg-1,6-bisp had an i50 value of 570 microm on aldolase activity, and it competed with fru-1 ... | 2002 | 12240991 |
| fusarium solani keratitis following lasik for myopia. | 2002 | 12234908 | |
| only specific tobacco (nicotiana tabacum) chitinases and [beta]-1,3-glucanases exhibit antifungal activity. | different isoforms of chitinases and [beta]-1,3-glucanases of tobacco (nicotiana tabacum cv samsun nn) were tested for their antifungal activities. the class i, vacuolar chitinase and [beta]-1,3-glucanase isoforms were the most active against fusarium solani germlings, resulting in lysis of the hyphal tips and in growth inhibition. in additon, we observed that the class i chitinase and [beta]-1,3-glucanase acted synergistically. the class ii isoforms of the two hydrolases exhibited no antifungal ... | 1993 | 12231736 |
| isolation of fusarium solani from a dog: identification by molecular analysis. | a strain of fusarium solani was isolated from a dog showing many cutaneous and submucosal nodules and pyogranulomatous kidney lesions. clinical isolates from this systemic infection were identified using microscopic examination and confirmed by molecular analysis. | 2002 | 12230226 |
| a case of melanonychia caused by fusarium solani. | 2002 | 12207614 | |
| electrophoretic karyotyping and gene mapping of seven formae speciales in fusarium solani. | chromosomal dnas of 22 strains in 7 formae speciales (f. spp.) of fusarium solani were compared by pulsed field gel electrophoresis (pfge) and gene mapping on the chromosomes. using pfge, complete separation of the full components of the genome was not attained, due to the limited resolution of large chromosomes, but 5-12 chromosomes with sizes of 0.6-5.7 mbp were resolvable for every strain. although each strain had a unique banding profile, similarity in the banding profile was noticed among s ... | 2002 | 12172966 |
| analysis of phylogenetic relationship of cylindrocarpon lichenicola and acremonium falciforme to the fusarium solani species complex and a review of similarities in the spectrum of opportunistic infections caused by these fungi. | an emerging pattern of similarity in medical case reports led to a project to compare the phylogenetic affinities of two well-known tropical fungal opportunistic pathogens, cylindrocarpon lichenicola and acremonium falciforme, to members of the fusarium solani species complex. c. lichenicola and a. falciforme, despite their deviating conidial morphologies, were shown via sequencing of the ribosomal large subunit to be well instituted within a clade mainly consisting of typical f. solani strains ... | 2002 | 12149344 |
| endogenous endophthalmitis following disseminated fungemia due to fusarium solani in a patient with acute myeloid leukemia. | we report the case of a young man with a resistant acute myeloid leukemia (aml) who developed a disseminated fungemia due to fusarium solani involving the skin and lungs, during the neutropenic phase following a chemotherapy course. despite continuous therapy with liposomal amphotericin b, he developed a bilateral endophthalmitis that rapidly evolved to complete blindness. the patient underwent two procedures of vitrectomy, with detection of f. solani in the vitreous fluid, and continued antifun ... | 2002 | 12144539 |
| canker and wilt of black locust (robinia pseudoacacia l.) caused by fusarium species. | from the pathological material of black locust trees showing symptoms of wilting of the foliage or canker of the bark the following fusarium species were isolated: fusarium avenaceum (fr.) sacc., fusarium lateritium nees., fusarium semitectum berk. & rav., fusarium solani (mart.) sacc., fusarium sulphureum schlecht. (syn.: fusarium sambucinum fuckel f. 6 wollenw.) the results of the provocation infections of one-year-old black locust seedlings showed that all of the species--except fusarium sola ... | 2002 | 12109155 |
| allergenic cross-reactivity of curvularia lunata with other airborne fungal species. | curvularia lunata is an important fungus for respiratory allergic disorders. previous studies indicated cross-reactivity of curvularia with other fungi. however, the cross-reactive allergenic component (s) were not identified. the present work was carried out to study the shared allergenic components of c. lunata and others. | 2002 | 12100306 |
| ocular and systemic posaconazole(sch-56592) treatment of invasive fusarium solani keratitis and endophthalmitis. | 2002 | 12084760 | |
| host recognition by pathogenic fungi through plant flavonoids. | a common characteristic among fungal pathogens of plants is that each specializes on a narrow range of specific plants as hosts. one adaptation to a specific host plant is the recognition of the host's chemicals which can be used to trigger genes or developmental pathways needed for pathogenesis. the production of characteristic flavonoids by plants, particularly those exuded from roots by legumes, appear to be used as signals for various microbes, including symbionts as well as pathogens. nectr ... | 2002 | 12083470 |
| alternate conformations observed in catalytic serine of bacillus subtilis lipase determined at 1.3 a resolution. | bacillus subtilis extracellular lipase (bsl) has an exceptionally low molecular weight (19.4 kda) for a member of the lipase family. a crystallographic study was performed on bsl in order to design and produce mutant bsl that will be more suitable for industrial uses based on analysis of the three-dimensional structure. recently, the crystal structure of bsl has been determined at 1.5 a resolution [van pouderoyen et al. (2001). j. mol. biol. 309, 215-226]. in the present study, a new crystal for ... | 2002 | 12077437 |
| in vitro activity of fk463, a novel lipopeptide antifungal agent, against a variety of clinically important molds. | the in vitro antifungal activity of fk463 against a variety of clinically important opportunistic molds was compared with amphotericin b, itraconazole and fluconazole by using the broth microdilution method m27-a specified by the national committee for clinical laboratory standards. fk463 exhibited potent activity against aspergillus species, which was superior to those of all other compounds tested. fk463 was also active against the dematiaceous fungi cladosporium trichoides, exophiala spinifer ... | 2002 | 12011539 |
| diversity in soil fungi from undisturbed and disturbed celtis tala and scutia buxifolia forests in the eastern buenos aires province (argentina). | the rhizospheric soil microfungi from a native forest (undisturbed and disturbed) were studied using soil dilution plate and soil washing methods. fungi were isolated using slightly acid and alkaline culture media. 54 taxa were isolated: 49 from undisturbed forest soil and 37 from disturbed forest soil. acremonium sp., aspergillus ustus, coemansia pectinata, doratomyces stemonitis, fusarium solani, f. oxysporum, gliocladium roseum, humicola fusco-atra, mortierella sp., penicillium lilacinum, tri ... | 2002 | 12002400 |
| substrate-regulated cyanide hydratase (chy) gene expression in fusarium solani: the potential of a transcription-based assay for monitoring the biotransformation of cyanide complexes. | the fungus fusarium solani detoxifies cyanide through induction of the cyanide hydratase gene activity (chy) in the presence of either kcn or the metal-complexed cyanides, k2ni(cn)4 or k4fe(cn)6, at ph 7.0 and 4.0 respectively. sequence analysis of the chy gene identified primers for reverse transcriptase-polymerase chain reaction (rt-pcr)-directed analysis of mrna transcripts, which demonstrated that activity correlated to the substrate-specific induction of gene expression. chy transcription w ... | 2002 | 12000318 |
| fusarium infections of the skin. | fusarium species are ubiquitous and may be found in the soil, air and on plants. fusarium species can cause mycotoxicosis in humans following ingestion of food that has been colonized by the fungal organism. in humans, fusarium species can also cause disease that is localized, focally invasive or disseminated. the pathogen generally affects immunocompromised individuals with infection of immunocompetent persons being rarely reported. localized infection includes septic arthritis, endophthalmitis ... | 2000 | 11964778 |