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mery-b, a metabolomic database and knowledge base for exploring plant primary metabolism.plant primary metabolites are organic compounds that are common to all or most plant species and are essential for plant growth, development, and reproduction. they are intermediates and products of metabolism involved in photosynthesis and other biosynthetic processes. primary metabolites belong to different compound families, mainly carbohydrates, organic acids, amino acids, nucleotides, fatty acids, steroids, or lipids. until recently, unlike the human metabolome database ( http://www.hmdb.ca ...201424218206
distribution and evolution of two satellite dnas in the genus beta.ecori monomers of a highly repetitive dna family of beta vulgaris have been cloned. sequence analysis revealed that the repeat length varies between 157-160 bp. the percentage of at-residues is 62% on average. the basic repeat does not show significant homology to the bamhi sequence family of b. vulgaris that was analyzed by us earlier. both the ecori and bamhi sequences are investigated and compared to each other with respect to their genomic organization in the genus beta. both repeats were fo ...199124213457
surcose transport in isolated plasma-membrane vesicles from sugar beet (beta vulgaris l.) evidence for an electrogenic sucrose-proton symport.an analysis of the molecular mechanism of sucrose transport across the plasmalemma was conducted with isolated plasma-membrane (pm) vesicles. plasma membrane was isolated by aqueous two-phase partitioning from fully expanded sugar beet (beta vulgaris l.) leaves. the isolated fraction was predominantly pm vesicles as determined by marker-enzyme analysis, and the vesicles were oriented right-side-out as determined by structurally linked latency of the pm enzyme, vanadate-sensitive mg(2+)-atpase. s ...198924212906
molecular characterization of mitochondrial dna of different subtypes of male-sterile cytoplasms of the sugar beet beta vulgaris l.mitochondrial (mt) dna from eight cytoplasmic male-sterile (cms) lines of sugar beet from different breeding stations was investigated by restriction fragment analysis and southern hybridization. all cms lines showed similar but not identical restriction and hybridization signal patterns, readily distinguishable from those of fertile (n) cytoplasm. digestion of the mtdna with bamhi, ecori, sali, and xhoi revealed distinct differences between the sterile lines, and six subtypes of the s cytoplasm ...199124212854
the quality of meat from sheep treated with tannin- and saponin-based remedies as a natural strategy for parasite control.lambs were assigned to four groups of seven and treated as follows for 12 days: control group (bp) was fed beet pulp; group t (tannin remedy) received the bp diet including 80 g/kg of quebracho extract; group s (saponin remedy) received the bp diet including 15 g/kg of quillaja extract; and group c had a free choice between t and s remedies. lipid oxidation was lower in meat from s lambs compared to t lambs (p<0.05). among the volatile compounds, lactate was lower in meat from s lambs compared t ...201424211542
evaluation of the fermentation of high gravity thick sugar beet juice worts for efficient bioethanol production.sugar beet and intermediates of sugar beet processing are considered to be very attractive feedstock for ethanol production due to their content of fermentable sugars. in particular, the processing of the intermediates into ethanol is considerably facilitated because it does not require pretreatment or enzymatic treatment in contrast to production from starch raw materials. moreover, the advantage of thick juice is high solid substance and saccharose content which eliminates problems with the st ...201324206573
a full set of monosomic addition lines in beta vulgaris from beta webbiana: morphology and isozyme markers.nine different monosomic additions in beta vulgaris from beta webbiana were characterized through morphological characters and isozyme markers. the effect of the alien chromosome on the morphology of the recipient species is chromosome specific, and nine morphotypes could be distinguished. the added chromosome caused a growth reduction in the recipient plants. eleven isozyme systems were used as marker systems. a 6pgdh band was found as a marker for chromosome 7, which contains a resistance gene ...199224203202
a linkage map of sugar beet (beta vulgaris l.).we have established a first linkage map for beets based on rflp, isozyme and morphological markers. the population studied consisted of 96 f2 individuals derived from an intraspecific cross. as was expected for outbreeding species, a relatively high degree of polymorphism was found within sugar beet; 47% of the dna markers were polymorphic for the chosen population. the map consists of 115 independent chromosomal loci designated by 108 genomic dna probes, 6 isozyme and one morphological marker. ...199224203039
determination of genetic stability using isozymes and rflps in beet plants regenerated in vitro.sugar, fodder and garden beet (beta vulgaris l.) plants have been regenerated in culture from a range of expiant material. of the regenerants 764 were subjected to isozyme analysis using eight enzyme-specific stains, and 60 were subjected to rflp analysis using three cdna probes. both molecular techniques allowed the identification of somaclonal variant plants. assessment of the numbers of variant isozymes and restriction fragments has allowed the calculation of the approximate percentage of var ...199224203036
transfer of resistance to the beet cyst nematode (heterodera schachtii schm.) into the brassica napus l. gene pool through intergeneric somatic hybridization with raphanus sativus l.an intergeneric somatic hybrid was obtained through peg-induced protoplast fusion between brassica napus l. (oil-seed rape, aacc, 2n=38) and a beet cyst nematode resistant genotype of raphanus sativus l. (fodder radish, rr, 2n=18). the hybrid nature of the regenerated plant was confirmed by flow cytometric analysis, rflp-analysis, and chromosome counts. southern blot analysis of total dna using pphcps1 (rbc-l) as probe indicated that the somatic hybrid contains chloroplasts of b. napus. the mito ...199224202768
fingerprinting plant genomes with oligonucleotide probes specific for simple repetitive dna sequences.oligonucleotides hybridizing to simple repetitive dna patterns are highly informative as probes for dna fingerprinting in all investigated animal species, including man. here we demonstrate the applicability of this technique in higher plants. the oligonucleotide probes (gtg)5 and (gata)4 were used to investigate the differences in dna fingerprint patterns of the following angiosperm species: triticum aestivum, secale cereale, hordeum vulgare, beta vulgaris, petunia hybrida, brassica oleracea, a ...199224202742
characterization of the nuclear ribosomal dna units and phylogeny of beta l. wild forms and cultivated beets.the nuclear rdna units of species belonging to the genus beta were characterized using heterologous probes of flax (entire unit and 25s) and sunflower (6.1-kb eco fragment containing the 18s, the entire intergenic spacer (igs) and a small piece of the 25s). the physical maps of one species from each section of the genus was constructed by localization of the ecori, bamhi, hindiii, kpni and saci restriction sites. for each species a single individual was used to obtain total dna. the major unit l ...199224202669
expression of mitochondrial genes in fertile and sterile sugar beet cytoplasms with different nuclear fertility restorer genes.variations in mitochondrial genome organization and in its expression between fertile, sterile sugar beet lines and fertile nuclear-restored plants were studied. southern blot hybridization with coxi, coxii, cob and atpa mitochondrial genes as probes showed that changes in the mitochondrial genome organization of sterile lines are associated with variations in the location of cob, atpa and coxii, but not coxi. when the coxii and atpa genes were used as hybridization probes, differences in the pr ...199124202361
growth response and element accumulation in beta vulgaris l. raised in fly-ash-amended soils.: fly-ash disposal is a major problem in and around coal-fired power plants. although the fly-ash contains several essential minerals, its application in agricultural practices is uncommon in india as elsewhere. the present study was undertaken to elucidate the possibility of fly-ash application to agricultural soils to improve crop yields. three different amounts of fly-ash (2, 4 and 8% w/w) were mixed with soil in 1 m(2) plots and seeds of beta vulgaris were sown in these soil-amended plots. p ...199424202126
localization of glyoxylate-cycle marker enzymes in peroxisomes of senescent leaves and green cotyledons.crude particulate homogenates from leaves of barley (hordeum vulgare l.), rice (oryza sativa l.), leaf-beet (beta vulgaris var. cicla l.) and pumpkin (cucurbita pepo l.) cotyledons were separated on sucrose density gradients. the peroxisomal fractions appeared at a buoyant density of 1.25 g·cm(-3) and contained most of the activities of catalase (ec 1.11.1.6), and hydroxypyruvate reductase (ec 1.1.1.81) on the gradients. in peroxisomal fractions from detached leaves and green cotyledons incubate ...199024202025
factors influencing t-dna transfer in agrobacterium-mediated transformation of sugarbeet.agrobacterium-mediated transformation of sugarbeet (beta vulgaris l.) was investigated for t-dna transfer efficiency, using an intron containing β-glucuronidase gene. preculture and coculture of hypocotyl and cotyledon explants with acetosyringone upon infection was studied. seven seed lots which included several hundred genotypes, were screened, and were all susceptible to t-dna transfer but with variable frequencies. cotyledon explants were more readily transformed than those from hypocotyls. ...199324201875
localization of a protein, immunologically similar to a sucrose-binding protein from developing soybean cotyledons, on the plasma membrane of sieve-tube members of spinach leaves.immunocytochemical studies using antibodies raised against a 62-kda membrane protein isolated from developing soybean (glycine max (l.) merr.) cotyledons were performed on leaf tissue of spinach (spinacia oleracea l.). this 62-kda protein was labeled by 6'-deoxy-6'-(4-azido-2-hydroxy)-benzamidosucrose (habs), a photoaffinity sucrose analogue (k. g. ripp et al., 1988, plant physiol.88, 1435-1445). western-blot analysis of spinach plasma-membrane proteins indicated a cross-reactive polypeptide ide ...198924201850
two different satellite dnas in beta vulgaris l.: evolution, quantification and distribution in the genus.two highly repeated ecori (0.45 × 10(6)) and bamhi (0.17 × 10(6)) fragments per haploid genome were found in sugar beet genomic dna. both fragments were located by 6% acrylamide-gel electrophoresis, purified and cloned in puc18. four of the inserts corresponding to each family were chosen for further study. both fragment families display the main characteristics of the satellite dna of animals and plants. the ecori and bamhi fragment families are arranged in long tandem arrays. fragments of the ...199224201508
plant regeneration from sugarbeet (beta vulgaris l.) hypocotyls cultured in vitro and flow cytometric nuclear dna analysis of regenerants.a simple and reproducible protocol for regeneration of sugarbeet plants from hypocotyl expiants derived from 21 day-old-seedlings has been developed. expiants were cultured on ms medium containing 0.3 mg/l n6-benzylaminopurine, 0.1 mg/l naphthalene acetic acid, 50 mg/l adenine and 0.5% (w/v) fructose, 0.5% (w/v) sucrose and 0.5% (w/v) glucose to induce the formation of organogenic calli (2.3% to 46.5% organogenic efficiency, depending on populations). shoot formation was induced in callus cultur ...199224201432
enzyme and storage protein electrophoresis in varietal identification of sugar beet.different methods of classification, based on total protein patterns as well as on specific isoenzyme patterns, were compared in order to establish an identification system for sugar beet varieties and lines. single seed patterns and bulk extractions of total and fractionated proteins were compared on sds-page. due to important intra-populational variation contrasting with similarity at the varietal level no method proved to be sufficiently discriminatory. twelve sugar beet lines have been genot ...199224197330
variation in mitochondrial translation products in fertile and cytoplasmic male-sterile sugar beets.intact and functional mitochondria were isolated from sugar beet plants (beta vulgaris l.) containing normal fertile (f) or cytoplasmic male-sterile (s1-s4) cytoplasms. incorporation of (35)s-methionine by mitochondria isolated from both roots and leaves showed approximately 20 major and ten minor translation products. comparison of the polypeptide synthesis patterns produced by leaf mitochondria from fertile plants of three different species within the genus beta revealed several taxonomically ...199224197296
improvement of protoplast culture protocols for beta vulgaris l. (sugar beet).the effects of nacl, feeder cells and the embedding of protoplasts in calcium alginate have been investigated in an attempt to improve culture conditions of recalcitrant sugar beet (beta vulgaris l.) mesophyll protoplasts. while the use of nacl in all instances proved detrimental to protoplast development, the other two treatments had clear beneficial effects. minimum plating densities, necessary to sustain cell division, could be reduced to <5% (<4000 protoplasts / ml) of the control levels and ...199324197260
characterisation of chloride transport at the tonoplast of higher plants using a chloride-sensitive fluorescent probe : effects of other anions, membrane potential, and transport inhibitors.the characteristics of cl(-) transport in isolated tonoplast vesicles from red-beet (beta vulgaris l.) storage tissue have been investigated using the cl(-)-sensitive fluorescent probe, 6-methoxy-1-(3-sulfonatopropyl)-quinolinium (spq). the imposition of (inside) positive diffusion potentials, generated with k(+) and valinomycin, increased the initial rate of cl(-) transport, demonstrating that cl(-) could be electrically driven into the vesicles. chloride influx was unaffected by so 4 (2-) , bu ...199024196819
glutamine synthetase oligomers and isoforms in sugarbeet (beta vulgaris l.).the α-amino-n compounds that accumulate in the thickening storage root of sugarbeet (beta vulgaris l.) were synthesized in the leaves (no 3 (-) nutrition) and also in the lateral roots (nh 4 (+) nutrition). ammonium stimulated glutamine synthetase (gs, ec 6.3.1.2) activity, especially in the lateral roots. with non-denaturing polyacrylamide-gel isoelectric focussing, simultaneously active charge-isomers of gs were separated in both leaves and roots. the leaf isoforms were active in an octameric ...199024196669
transfer of resistance to the beet cyst nematode (heterodera schachtii schm.) from sinapis alba l. (white mustard) to the brassica napus l. gene pool by means of sexual and somatic hybridization.sexual and somatic hybrid plants have been produced between sinapis alba l. (white mustard) and brassica napus l. (oil-seed rape), with the aim to transfer resistance to the beet cyst nematode heterodera schachtii schm. (bcn) from white mustard into the oil-seed rape gene pool. only crosses between diploid accessions of s. alba (2n = 24, sa1sa1) as the pistillate parent and several b. napus accessions (2n = 38, aacc) yielded hybrid plants with 31 chromosomes. crosses between tetraploid accession ...199324196037
dna fingerprinting in sugar beet (beta vulgaris) - identification of double-haploid breeding lines.the distribution and abundance of simple repetitive sequences complementary to the synthetic oligonucleotides (gaca)4, (gata)4, (gtg)5 and (ca)8 in the genomes of several cultivars of beta vulgaris and in the wild beet b. vulgaris ssp. maritima were investigated. hybridization experiments revealed that all four motifs were present, though at different abundances, in the genomes of all of the investigated beet cultivars. considerable intraspecific variation of the resulting dna fingerprints was o ...199324196032
different cms sources found in beta vulgaris ssp maritima: mitochondrial variability in wild populations revealed by a rapid screening procedure.mitochondrial dna (mtdna) variation in natural beta maritima populations has been characterized by way of southern blot hybridizations of total dna using non-radioactive probes and chemiluminescent detection. it was found that the previously described n ("normal") mitochondrial type could be subdivided into three subtypes. a new mitochondrial genotype (type r) was distinguished in addition to the previously described type s. both are male-sterile cytoplasms and can produce a. segregation of sexu ...199324195925
relationships between genotype x environment interactions and rank orders for a set of genotypes tested in different environments.multilocation trials in plant breeding lead to cross-classified data sets with rows=genotypes and columns=environments, where the breeder is particularly interested in the rank orders of the genotypes in the different environments. non-identical rank orders are the result of genotype x environment interactions. not every interaction, however, causes rank changes among the genotypes (rank-interaction). from a breeder's point of view, interaction is tolerable only as long as it does not affect the ...199324194001
induction, purification and characterization of chitinase isolated from pea leaves inoculated with ascochyta pisi.chitinase (ec 3.2.1.14.) activity increased in pea (pisum sativum l.) leaves inoculated with both virulent and avirulent isolates of ascochyta pisi lib. three basic chitinase isoenzymes were purified: two, a1 and a2, separated by high performance liquid chroma tography, had a relative molecular mass (mr) of approx. 34 000, with an isoelectric point (pi) of 8.5, and one, b, had an mr of approx. 25 000, with a pi of 9.0. elevated levels of chitinase isoenzymes were found in the leaves: thus 150 h ...199124193924
sucrose-dependent h(+) transport in plasma-membrane vesicles isolated from sugarbeet leaves (beta vulgaris l.) : evidence in support of the h(+)-symport model for sucrose transport.the mechanism of sucrose transport across the plasma membrane (pm) was investigated in membrane vesicles isolated from sugarbeet (beta vulgaris l.) leaves. in the presence of a membrane potential (δψ) generated as a k(+)-diffusion potential, negative inside, sucrose induced a rapid and transient alkalization of the medium. alkalization was inhibited by carbonyl cyanide m-chlorophenylhydrazone, was specific for the sucrose sugar and was dependent on the sucrose concentration with a km of approx. ...199124193852
one-pot conversions of raffinose into furfural derivatives and sugar alcohols by using heterogeneous catalysts.inedible and/or waste biomass reserves are being strongly focused upon as a suitable new energy and chemical source. raffinose, which is an indigestible trisaccharide composed of glucose, galactose, and fructose, is found abundantly in beet molasses, sugar cane, and seeds of many leguminous plants. herein, we demonstrate the one-pot synthesis of furan derivatives and sugar alcohols from raffinose by using heterogeneous acid, base, and/or metal-supported catalysts. the combination of amberlyst-15 ...201424193816
isozyme analysis of primary trisomies in beet (beta vulgaris l.). genetical characterization and techniques for chromosomal assignment of two enzyme-coding loci: leucine aminopeptidase and glutamate oxaloacetate transaminase.segregating families of beet (beta vulgaris) were used to verify the monofactorial inheritance of two enzyme-coding loci, leucine aminopeptidase (lap1) and glutamate oxaloacetate transaminase (got3). with a series of primary trisomies and using three methods to discriminate between the critical trisomic (the locus is situated on the triplicated chromosome) and the non-critical ones, it was possible to allocate the two loci to beet chromosomes i and ii, respectively. for the locus lap1 distorted ...199324193787
rearrangements in sugar beet mitochondrial dna induced by cell suspension, callus cultures and regeneration.structural alterations in mitochondrial dnas (mtdnas) from a plant of a sterile sugar beet line, callus derived from it, suspension-cultured cells and plants regenerated from the callus were studied. bamhi restriction analysis revealed that structural alterations between the mtdnas of the callus and the control plant had occurred. multiple rearrangements were also demonstrated in the mtdna from the suspension culture, of which some were similar to those appearing in the callus, and others had ar ...199324193779
phylogenetic relationships between cultivated and wild species of the genusbeta revealed by dna "fingerprinting".forty-one accessions of the genusbeta representing wild and cultivated species of all sections were analyzed by dna "fingerprinting". four sugar beet minisatellite dna probes revealed characteristic banding patterns with southern-hybridizedbeta dna restricted withhindiii. a total of 111 polymorphic rflp bands were scored across all accessions. cluster analysis based on genetic similarity estimates for all 820 combinations of accessions revealed the following results. (1) all accessions could una ...199324193592
changes in ruminal fermentation, milk performance and milk fatty acid profile in dairy cows with subacute ruminal acidosis and its regulation with pelleted beet pulp.the aims of the experiment were to investigate the variation in ruminal fermentation, milk performance and milk fatty acid profile triggered by induced subacute ruminal acidosis (sara); and to evaluate the ability of beet pulp (bp) as a replacement for ground maize in order to alleviate sara. eight holstein-friesian cows were fed four diets (total mixed rations) during four successive periods (each of 17 d): (1) without wheat (w0); (2) with 10% finely ground wheat (fgw) (w10); (3) with 20% fgw ( ...201324192077
the origin and evolution of weed beets: consequences for the breeding and release of herbicide-resistant transgenic sugar beets.populations of weed beets have expanded into european sugar beet production areas since the 1970s, thereby forming a serious new weed problem for this crop. we sampled seeds in different french populations and studied mitochondrial dna, chloroplast dna and life-cycle variability. given the maternal inheritance of the mitochondrial and chloroplastic genomes and the nuclear determinism of the annual habit, we were able to determine the maternal origin and evolution of these weed beet populations. ...199324190320
the effect of gametic-phase disequilibrium on the prediction of response to recurrent selection in plants.selection reduces additive genetic variation by generating gametic-phase disequilibrium, a phenomenon largely ignored when predicting response in plant breeding programs. the development of gametic-phase disequilibrium is here taken into account when predicting the response to selection for various schemes of recurrent selection applicable to plant populations. a general program permits prediction of response to selection from schemes of recurrent selection in which two or more rounds of selecti ...199324190207
transmission via plants of an insect pathogenic bacterium that does not multiply or move in plants.a bacterial parasite (designated as bev) of the leafhopper euscelidius variegatus, which is passed transovarially to offspring, was transmitted from insect to insect via feeding of the insects in plants. the rate of bacterial infection of leafhoppers fed upon plants that had previously been exposed to bev-infected leafhoppers declined with an increase in the time that infected leafhoppers had been off rye grass. transmission of bev also occurred on sugar beet and barley but not celery. the bacte ...199424190165
characterization of solute/proton cotransport in plasma membrane vesicles from ricinus cotyledons, and a comparison with other tissues.plasma membrane vesicles, purified by aqueous two-phase partitioning, were used to investigate the presence of sugar and amino acid carriers in cotyledons and roots of ricinus communis l. and in roots of red beet (beta vulgaris l.). artificial ph and electrical gradients were generated across the plasma membrane, and [(14)c]acetate and [(14)c]tetraphenylphosphonium were used to demonstrate the presence of an internal alkaline ph gradient and an internal negative membrane potential, respectively. ...199224186784
sucrose transport in tonoplast vesicles of red beet roots is linked to atp hydrolysis.sucrose uptake into tonoplast vesicles, which were prepared from red beet (beta vulgaris l.) vacuoles isolated by two different methods, was stimulated by mgatp. using the same medium as for osmotic disruption of vacuoles, membrane vesicles were prepared from tissue homogenates of dormant red beet roots and separated by high-speed centrifugation through a discontinuous dextran gradient. a low-density microsomal fraction highly enriched in tonoplast vesicles could be further purified from contami ...199124186350
identification of rflp markers closely linked to the bolting gene b and their significance for the study of the annual habit in beets (beta vulgaris l.).the annual habit in beet is due to complete or partial absence of the vernalization requirement and can cause severe problems in the beet crop. the absolute vernalization requirement in beet is controlled by a major geneb (bolting), known to be linked to the gener (red hypocotyl color), in linkage group i. segregation for theb andr genes was studied in several beet progenies. penetrance of the annual habit inbb genotypes was affected by both environmental and genetic factors. the precise locatio ...199424186188
dna fragments of organellar origin in random amplified polymorphic dna (rapd) patterns of sugar beet (beta vulgaris l.).the technique of random amplified polymorphic dna (rapd) offers a broad range of applications in the investigation of plant genomes. a promising prospect is the use of rapd products as genetic markers. we have investigated a possible organellar source of fragments in rapd patterns of total dna. two nearly-isogenic lines of cytoplasmic male-sterile and male-fertile sugar beet (beta vulgaris l.) were subjected to rapd analysis with six different primers. total, nuclear, mitochondrial (mt), and chl ...199424186177
physical mapping of rrna genes by fluorescent in-situ hybridization and structural analysis of 5s rrna genes and intergenic spacer sequences in sugar beet (beta vulgaris).a digoxigenin-labelled 5s rdna probe (pta-794) and a rhodamine-labelled 18s-5.8s-25s rdna probe (pta71) were used for double-target in-situ hybridization to root-tip metaphase, prophase and interphase chromosomes of cultivated beet,beta vulgaris l. after in-situ hybridization with the 18s-5.8s-25s rdna probe, one major pair of sites was detected which corresponded to the secondary constriction at the end of the short arm of chromosome 1. the two rdna chromosomes were often associated and the loc ...199424186156
construction and characterisation of a yeast artificial chromosome library containing five haploid sugarbeet (beta vulgaris l.) genome equivalents.a yeast artificial chromosome (yac) genomic library of beta vulgaris was constructed in the pyac4 vector. high-molecular-weight dna was prepared from agarose-embedded leaf protoplasts from a triploid cultivar. the library was found to contain 33,500 clones in an ordered array of microtiter plates. mean size of the inserts was estimated to be 135 kb, and the library should therefore represent the equivalent of five haploid genomes. the library was characterised for the presence of highly repetiti ...199424186033
versatile solvent systems for the separation of betalains from processed beta vulgaris l. juice using counter-current chromatography.two mixtures of decarboxylated and dehydrogenated betacyanins from processed red beet roots (beta vulgaris l.) juice were fractionated by high performance counter-current chromatography (hpccc) producing a range of isolated components. mixture 1 contained mainly betacyanins, 14,15-dehydro-betanin (neobetanin) and their decarboxylated derivatives while mixture 2 consisted of decarboxy- and dehydro-betacyanins. the products of mixture 1 arose during thermal degradation of betanin/isobetanin in mil ...201324184837
the detection of somaclonal variants of beet using rapd.plantlets were regenerated by adventitious shoot budding in tissue culture from leaf explants of a single genotype of sugar beet. dna was extracted from the parental plant and from 120 regenerants. rapd analysis was carried out using five decanucleotide primers; 4,557 rapd marker bands were examined and two polymorphisms were observed. thirty secondary regenerants were then derived, using the same tissue culture technique, from thirty of the primary regenerants. again rapd analysis was employed ...199624178456
antiauxin enhanced microshoot initiation and plant regeneration from epicotyl-originated thin-layer explants of sugarbeet (beta vulgaris l.).an in vitro method was developed for microshoot initiation from thin-layer explants prepared from the elongated epicotyls of sugarbeet (beta vulgaris l.). intact epicotyls of 14-day-old seedlings were excised from the hypocotyls above the cotyledons and allowed to elongate on de greef and jacobs (1979) medium supplemented with 0.2 mg/l 6-benzyladenine, 0.2 mg/l gibberellic acid and 0.1 mg/l indole-3-acetic acid in darkness. after a 21-day-incubation, the elongated epicotyls were halved to obtain ...199624178223
effect of promoter-leader sequences on transient expression of reporter gene chimeras biolistically transferred into sugarbeet (beta vulgaris) suspension cells.chimeric constructs consisting of the gus coding region fused downstream of promoterun-translated leader sequences from the tobacco osmotin and pr-s genes, the potato proteinase inhibitor 2 gene (pin2), and the cauliflower mosaic virus (camv) 35s promoter were biolistically transferred into sugarbeet suspension cells. each construct was expressed in recipient cells at 6 h after bombardment with maximum levels observed between 12 and 48 h. expression of the pr-s construct mimicked the time-course ...199624178220
induction and purification of chitinase in brassica napus l. ssp. oleifera infected with phoma lingam.a pathogen-induced chitinase (ec 3.2.1.14) was isolated from cotyledons of oilseed rape (brassica napus cv. bienvenu) 8 d after inoculation with phoma lingam. the purified chitinase has a molecular weight of 30 kda, and an isoelectric point of approx. 9.1. a partial amino-acid sequence obtained after tryptic digestion of the protein shows high sequence similarity to basic chitinases from bean, tobacco, potato, arabidopsis, barley and rice, as well as to acidic chitinases from tobacco and petunia ...199224178073
long-range organization of a satellite dna family flanking the beet cyst nematode resistance locus (hs1) on chromosome-1 of b. patellaris and b. procumbens.new members of a satellite dna family (sat 121), specific for wild beets of the section procumbentes of the genus beta, were isolated. sequence analysis showed that the members of sat-121 fall into two distinct classes. the organization of sat-121 in the vicinity of a beet cyst nematode (heterodera schachtii schm.) resistance locus (hs1) in b. patellaris and b. procumbens was investigated by pulsed-field gel electrophoresis (pfge) using dna from a series of resistant monosomic fragment additions ...199424177895
construction of a yac library from a beta vulgaris fragment addition and isolation of a major satellite dna cluster linked to the beet cyst nematode resistance locus hs1 (pat-1.).a yac library was constructed from the beta vulgaris fragment addition an5-203b. this monosomic fragment addition harbors an approximate 12-mbp fragment of b.patellaris chromosome 1 accomodating the hs1 (pat-1) conferring resistance to the beet cyst nematode (heterodera schachtii). the yac library consists of 20,000 yac clones having an average size of 140 kb. screening with organelle-specific probes showed that 12% of the clones contain chloroplast dna while only 0.2% of the clones hybridizes w ...199424177891
molecular heterogeneity in mitochondrial and chloroplast dnas from normal and male sterile cytoplasms in sugar beets.mitochondrial (mt) and chloroplast (ct) dnas were prepared from normal (n) and male sterile (s) cytoplasmic lines of sugar beet. the dnas were cleaved with bamhi, ecori, hindiii and sali enzymes, and the resultant fragments were fractionated by agarose gel electrophoresis. the results showed that n and s cytoplasms contained distinct mtdna. although most of the dna fragments were common to these two cytoplasms, each cytoplasm was readily characterized by bands specific to that cytoplasm. in addi ...198424177801
in planta 2,3,5 truodobenzoic acid treatment promotes high frequency and routine in vitro regeneration of sugarbeet (beta vulgaris l.) plants.the effect ofin planta treatments with auxin inhibitors such as 2,3,5 triiodobenzoic acid (tiba) on regeneration of plantsin vitro is not known. here, we show the beneficial effect of preconditioning sugarbeet plants in the greenhouse with tiba (3 mg/1) for efficientin vitro plant regeneration via a callus phase from cultured leaf explants. without this treatment, no shoot developed on the control leaf-calluses. several hundred plants were routinely regenerated using this protocol. more importan ...199624177540
effect of poly-l-lysine on potassium fluxes in red beet tissue.poly-l-lysine concentrations (10(-6) m) which cause slight leakage of pigment from beet cells completely disrupt the kinetics of(*)k (labeled) absorption at 25°c in the range 0.01 to 50mm kcl. lower concentrations of polylysine (10(-7) to 10(-9) m) interfere with potassium fluxes at both cell membranes, initially increasing efflux across the plasma membrane and decreasing the capacity of the cytoplasm to retain ions during flux experiments at 2°c. at 25°c, these concentrations of polylysine incr ...197024174139
physical mapping and cloning of a translocation in sugar beet (beta vulgaris l) carrying a gene for nematode (heterodera schachtii) resistance from b. procumbens.two diploid (2n=18) sugar beet (beta vulgaris l.) lines which carry monogenic traits for nematode (heterodera schachtii schm.) resistance located on translocations from the wild beet species beta procumbens were investigated. short interspersed repetitive dna elements exclusively hybridizing with wild beet dna were found to be dispersed around the translocations. the banding pattern as revealed by genomic southern hybridization was highly conserved among translocation lines of different origins ...199524173930
an extended map of the sugar beet genome containing rflp and rapd loci.an updated map of sugar beet (beta vulgaris l. ssp. vulgaris var 'altissima doell') is presented. in this genetic map we have combined 248 rflp and 50 rapd loci. including the loci for rhizomania resistance rr1, hypocotyl colour r and the locus controlling the monogerm character m, 301 loci have now been mapped to the nine linkage groups covering 815 cm. in addition, the karyotype of some of the beta vulgaris chromosomes has been correlated with existing rflp and rapd linkage maps.199524173890
isolation and characterization of rapd-based markers linked to the beet cyst nematode resistance locus (hs1 (pat-1)) on chromosome 1 of b. patellaris.a beet cyst nematode (bcn)-resistant telosomic addition of b. patellaris chromosome 1 in b. vulgaris was used to isolate 6 rapd markers linked to the bcn resistance locus hs1 (pat-1). southern analysis showed that the analyzed rapd products contain either low-, middle or high-repetitive dna. the relative positions of the random amplified polymorphic dna (rapd) markers and of the restriction fragment length polymorphism (rflp) loci corresponding to the low-repetitive rapd products were determined ...199524172934
[special effects of a complex probiotic containing cellulolytic bacteria cellulomonas on actively growing rabbits].it was shown that the association of probiotic bacteria of the genuses bacillus and cellulomonas form biolayers on the surface of beet marc particles. the positive effect of a fodder additive that contained the biolayer on the basis of a phytomatrix on the growth and development of young rabbits was shown. feeding of animals with a mixed fodder that contained 0.1% preparation resulted in stimulation of digestion of all components of the food. among other components of the mixed fodder, cellulose ...201624171309
mapping of a chloroplast rflp marker associated with the cms cytoplasm of sugar beet (beta vulgaris).the owen cytoplasm of male-sterile sugar beet is associated with several alterations of mitochondrial dna and one additional hindiii site of chloroplast dna. the region of this hindiii site has been cloned and sequenced. the site maps in a small reading frame (orf32) close to the ycf7 (orf31) gene in the petg-psbe region of chloroplast dna. possible functional implications of the results are discussed. the chloroplast rflp marker described could be useful for studies on chloroplast-mitochondrial ...199524169966
genetic localization of four genes for nematode (heterodera schachtii schm.) resistance in sugar beet (beta vulgaris l.).sugar beet (beta vulgaris l.) is highly susceptible to the beet cyst nematode (heterodera schachtii schm.). three resistance genes originating from the wild beets b. procumbens (hs1 (pro-1)) and b. webbiana (hs1 (web-1), hs2 (web-7)) have been transferred to sugar beet via species hybridization. we describe the genetic localization of the nematode resistance genes in four different sugar beet lines using segregating f2 populations and rflp markers from our current sugar beet linkage map. the map ...199624166626
abundance and length polymorphism of microsatellite repeats in beta vulgaris l.simple sequence repeats (ssrs) are known to exhibit high degrees of variability even among closely related individuals. their usage as nuclear genetic markers requires their conversion into sequence-tagged sites (stss). in this paper we present the development of simple sequences as stss for beta vulgaris. this species comprises wild, cultivated, and weedy forms; the latter are thought to originate from accidental hybridisation between the other two. two partial genomic libraries were screened w ...199624166253
characterization of nematode resistance genes in the section procumbentes genus beta: response to two populations of heterodera schachtii.three species of the section procumbentes genus beta, nine monosomic additions, and five translocation lines were tested for resistance to two heterodera schachtii populations. nematode population 129-v (129-virulent) was selected for virulence to resistance gene(s) transferred from chromosome 1 of beta procumbens to the diploid resistant sugar beet kws-nr1. this population is considered to be a pathotype. the unselected sib population 129-av (129-avirulent) was reared continuously on fodder rap ...199624162407
[effect of triton x-100 on genetic segregation and associated monocotyledonous and dicotyledonous traits in sugarbeet (beta vulgaris l.)].the effect of triton x-100 (tx-100) on the ratio of phenotypic classes and the expression of morphological traits in the progeny of sugar beet hybrids (n12 and n2) was investigated. it was shown that the tx-100 exposition on the unopened flower buds of sugar beets has different effects on hybrid progenies. in agamospermic progeny of hybrid plant no 12km-4, a significant decrease in the heteroallelic (heterozygous) phenotypic classes of alcohol dehydrogenase (adh1) fraction was determined in the ...201324159801
immobilized sclerotinia sclerotiorum invertase to produce invert sugar syrup from industrial beet molasses by-product.the fungus sclerotinia sclerotiorum produces invertase activity during cultivation on many agroindustrial residues. the molasses induced invertase was purified by deae-cellulose chromatography. the molecular mass of the purified enzyme was estimated at 48 kda. optimal temperature was determined at 60 °c and thermal stability up to 65 °c. the enzyme was stable between ph 2.0 and 8.0; optimum ph was about 5.5. apparent k(m) and v(max) for sucrose were estimated to be respectively 5.8 mm and 0.11 μ ...201424142426
hydroxymethylfurfural: a possible emergent cause of honey bee mortality?hydroxymethylfurfural (hmf), a common product of hexose degradation occurring during the maillard reaction and caramelization, has been found toxic for rats and mice. it could cause a potential health risk for humans due to its presence in many foods, sometimes exceeding 1 g/kg (in certain dried fruits and caramel products), although the latter still is controversial. hmf can also be consumed by honey bees through bad production batches of sugar syrups that are offered as winter feeding. in belg ...201324127696
myo-inositol oxygenase is important for the removal of excess myo-inositol from syncytia induced by heterodera schachtii in arabidopsis roots.the enzyme myo-inositol oxygenase is the key enzyme of a pathway leading from myo-inositol to udp-glucuronic acid. in arabidopsis, myo-inositol oxygenase is encoded by four genes. all genes are strongly expressed in syncytia induced by the beet cyst nematode heterodera schachtii in arabidopsis roots. here, we studied the effect of a quadruple myo-inositol oxygenase mutant on nematode development. we performed metabolite profiling of syncytia induced in roots of the myo-inositol oxygenase quadrup ...201424117492
effects of 8 weeks of xpand® 2x pre workout supplementation on skeletal muscle hypertrophy, lean body mass, and strength in resistance trained males.xpand® 2x is a proprietary blend comprised of branched chain amino acids, creatine monohydrate, beta-alanine (carnosyn®), quercetin, coenzymated b-vitamins, alanyl-glutamine (sustamine®), and natural nitrate sources from pomegranate and beet root extracts purported to enhance the neuromuscular adaptations of resistance training. however to date, no long-term studies have been conducted with this supplement. the purpose of this study was to investigate the effects of a multi-ingredient performanc ...201324107586
isolation and characterization of burkholderia rinojensis sp. nov., a non-burkholderia cepacia complex soil bacterium with insecticidal and miticidal activities.isolate a396, a bacterium isolated from a japanese soil sample demonstrated strong insecticidal and miticidal activities in laboratory bioassays. the isolate was characterized through biochemical methods, fatty acid methyl ester (fame) analysis, sequencing of 16s rrna, multilocus sequence typing and analysis, and dna-dna hybridization. fame analysis matched a396 to burkholderia cenocepacia, but this result was not confirmed by 16s rrna or dna-dna hybridization. 16s rrna sequencing indicated clos ...201324096416
exploitation of fta cartridges for the sampling, long-term storage, and dna-based analyses of plant-parasitic nematodes.the use of dna-based analyses in molecular plant nematology research has dramatically increased over recent decades. therefore, the development and adaptation of simple, robust, and cost-effective dna purification procedures are required to address these contemporary challenges. the solid-phase-based approach developed by flinders technology associates (fta) has been shown to be a powerful technology for the preparation of dna from different biological materials, including blood, saliva, plant t ...201424093923
feed intake, nutrient digestibility and ruminal fermentation activities in sheep-fed peanut hulls treated with trichoderma viride or urea.this study aimed to assess impacts of fungal treatment on the nutritional value of peanut hulls (ph) or urea at the rate of 5 kg/100 g of ph. fermented sugar beet pulp inoculated with trichoderma viride was supplemented to ph at rates of 5.0, 10.0 and 15.0 g/100 g air dry of ph and mixed well before aerobic incubation for 21 days. organic matter (om) content of ph declined with increased levels of fermented sugar beet pulp inoculums, while crude protein (cp), ether extract (ee), and ash increase ...201424085418
sugarcane vinasse: environmental implications of its use.the inadequate and indiscriminate disposal of sugarcane vinasse in soils and water bodies has received much attention since decades ago, due to environmental problems associated to this practice. vinasse is the final by-product of the biomass distillation, mainly for the production of ethanol, from sugar crops (beet and sugarcane), starch crops (corn, wheat, rice, and cassava), or cellulosic material (harvesting crop residues, sugarcane bagasse, and wood). because of the large quantities of vina ...201324084103
a class of compartmental models for long-distance tracer transport in plants.studies of long-distance tracer transport in plants result in spatio-temporal data sets. compartmental tracer transport models can be used to quantitatively characterize or compare such data sets derived from different experiments. depending on the specific experimental situation it might be necessary to apply different models. here, we present a general class of compartmental tracer transport models which allows a systematic comparison of different models regarding the quality of fitting to the ...201424076452
changes induced by zinc toxicity in the 2-de protein profile of sugar beet roots.changes induced by three levels of zn toxicity in the root proteome from beta vulgaris were studied by two dimensional gel electrophoresis. 320 spots were consistently detected and 5, 5 and 11% of them showed significant changes in relative abundance as a result of the 50, 100 and 300μm zn treatments, respectively, when compared to controls (1.2μm zn). forty-four spots had consistent changes between all treatments, and 93% were identified. at low and mild zn excess, the complex i of the mitochon ...201324056184
influence of psyllium, sugar beet fibre and water on gluten-free dough properties and bread quality.celiac patients generally have a low intake of protein and fibre attributed to their gluten-free (gf) diet. to satisfy the increasing demand for healthier products, this research focused on the effects of the supplementation of psyllium (p) and sugar beet fibre (sb) on the mixing and leavening behaviour of gluten-free doughs. four doughs, having different consistencies that made them suitable to be poured into moulds or to be shaped, and their corresponding breads were evaluated. the results obt ...201324053854
genomic selection in sugar beet breeding populations.genomic selection exploits dense genome-wide marker data to predict breeding values. in this study we used a large sugar beet population of 924 lines representing different germplasm types present in breeding populations: unselected segregating families and diverse lines from more advanced stages of selection. all lines have been intensively phenotyped in multi-location field trials for six agronomically important traits and genotyped with 677 snp markers.201324047500
economic feasibility of the sugar beet-to-ethylene value chain.as part of a long-term strategy toward renewable feedstock, a feasibility study into options for the production of bioethylene by integrating the sugar beet-to-ethanol-to-ethylene value chain. seven business cases were studied and tested for actual economic feasibility of alternative sugar-to-ethanol-to-ethylene routes in comparison to fossil-fuel alternatives. an elaborate model was developed to assess the relevant operational and financial aspects of each business case. the calculations indica ...201324039080
characterization of drought-tolerant sugar beet mutants induced with gamma radiation using biochemical analysis and isozyme variations.in this study, drought-tolerant mutants of sugar beet (beta vulgaris l. cv. felicita) were obtained by in vitro mutagenesis and characterized by biochemical analysis and isozyme variations.201424037781
effect of acid hydrolysis and fungal biotreatment on agro-industrial wastes for obtainment of free sugars for bioethanol production.this study was designed to evaluate selected chemical and microbiological treatments for the conversion of certain local agro-industrial wastes (rice straw, corn stalks, sawdust, sugar beet waste and sugarcane bagasse) to ethanol. the chemical composition of these feedstocks was determined. conversion of wastes to free sugars by acid hydrolysis varied from one treatment to another. in single-stage dilute acid hydrolysis, increasing acid concentration from 1 % (v/v) to 5 % (v/v) decreased the con ...201224031984
insecticide resistance status of field populations of spodoptera exigua (lepidoptera: noctuidae) from china.beet armyworm, spodoptera exigua (hübner), is a serious pest of vegetables in china, and its control is heavily dependent on chemical insecticides. the current resistance status of nine insecticides was investigated in 16 field populations collected from seven provinces of china during 2009-2012. compared with the susceptible strain wh-s, some field populations evolved various levels of resistance to eight of the nine insecticides tested: emamectin benzoate (4- to 348-fold), indoxacarb (2- to 41 ...201324020303
lethal and sublethal effects of spinosad on spodoptera exigua (lepidoptera: noctuidae).as a selective biological insecticide, spinosad has been used widely for the control of pests including beet armyworm, spodoptera exigua (hübner) (lepidoptera: noctuidae). to form effective pest control strategies, lethal and sublethal effects should be considered for a complete analysis of spinosad impact. however, few studies have been reported to investigate sublethal effects of spinosad on s. exigua. this study attempts to evaluate the lethal and sublethal effects of spinosad on this pest by ...201324020299
beetroot-pigment-derived colorimetric sensor for detection of calcium dipicolinate in bacterial spores.in this proof-of-concept study, we describe the use of the main red beet pigment betanin for the quantification of calcium dipicolinate in bacterial spores, including bacillus anthracis. in the presence of europium(iii) ions, betanin is converted to a water-soluble, non-luminescent orange 1∶1 complex with a stability constant of 1.4 × 10(5) l mol(-1). the addition of calcium dipicolinate, largely found in bacterial spores, changes the color of the aqueous solution of [eu(bn)(+)] from orange to m ...201324019934
phenolic content, antioxidant and antibacterial activity of selected natural sweeteners available on the polish market.seventeen natural sweeteners available on the polish market were screened for total phenolic content, by the folin-ciocalteu method, and for antioxidant activity, using the ferric reducing antioxidant power (frap) assay and the 2,2'-azinobis (3-ethylbenzthiazoline-6-sulphonic acid) radical cation decolorization assay (abts(·+)). in addition, we analyzed antibacterial activities against staphylococcus aureus strains: both those susceptible and those resistant to methicillin (mrsa). the results of ...201324007486
effects of biomass types and carbonization conditions on the chemical characteristics of hydrochars.effects of biomass types (bark mulch versus sugar beet pulp) and carbonization processing conditions (temperature, residence time, and phase of reaction medium) on the chemical characteristics of hydrochars were examined by elemental analysis, solid-state ¹³c nmr, and chemical and biochemical oxygen demand measurements. bark hydrochars were more aromatic than sugar beet hydrochars produced under the same processing conditions. the presence of lignin in bark led to a much lower biochemical oxygen ...201324004410
screening of extracts from natural feed ingredients for their ability to reduce enterotoxigenic escherichia coli (etec) k88 adhesion to porcine intestinal epithelial cell-line ipec-j2.enterotoxigenic escherichia coli (etec) k88 is the most prevalent enteropathogen in weaned piglets, with the ability to express fimbria f4 and specifically attach to intestinal receptors in the young piglet. the prevention of etec k88 adhesion to the epithelium by interfering in this fimbria-receptor recognition provides an alternative approach to prevent the initial stage of disease. the aim of this study is to screen, among different feed ingredients (fi), their ability to reduce etec k88 atta ...201323992796
effects of high fiber intake during late pregnancy on sow physiology, colostrum production, and piglet performance.dietary fiber given during pregnancy may influence sow endocrinology and increase piglet bw gain during early lactation. the aim of the current study was to determine whether dietary fiber given to sows during late pregnancy induces endocrine changes that could modulate sow colostrum production and, thus, piglet performance. from d 106 of pregnancy until parturition, 29 landrace×large white nulliparous sows were fed gestation diets containing 23.4 [high fiber (hf); n=15] or 13.3% total dietary f ...201323989876
expression of soybean lectin in transgenic tobacco results in enhanced resistance to pathogens and pests.lectins are proteins of non-immune origin that specifically interact with carbohydrates, known to play important roles in the defense system of plants. in this study, in order to study the function of a new soybean lectin (sbl), the corresponding encoding gene lec-s was introduced into tobacco plants via agrobacterium-mediated transformation. southern blot analyses had revealed that the lec-s gene was stable integrated into the chromosome of the tobacco. the results of the reverse transcription ...201323987807
extraction, characterization and spontaneous emulsifying properties of pectin from sugar beet pulp.the effects of organic acid extractants on the yield and characteristics of pectin from sugar beet pulp were investigated with citric acid, malic acid and lactic acid at different ph (1.5 and 2.0) and time (1 h and 2 h). the results demonstrated that the yields of pectins were directly correlated with the decrease of ph and reaction time, and the optimum yield of 17.2% was obtained at ph 1.5 and 2 h. furthermore, the acid type also affected the physicochemical characteristics of pectin, especial ...201323987408
phase separation induced molecular fractionation of gum arabic--sugar beet pectin systems.this paper investigates the phase separation and phase separation-induced fractionation of gum arabic (ga)/sugar beet pectin (sbp) mixed solutions. a phase diagram, including cloud and binodal curves, was established by visual observation and phase composition analysis. the deviation of the binodal curve from the cloud curve was a result of phase separation-induced fractionation of polydisperse ga and sbp molecules. fractionation of ga increased the content of arabinogalactan-protein complex (ag ...201323987401
influence of layer thickness and composition of cross-linked multilayered oil-in-water emulsions on the release behavior of lutein.multilayering and enzymatic cross-linking of emulsions may cause alterations in the release behavior of encapsulated core material due to changes in thickness, porosity and permeability of the membrane. an interfacial engineering technology based on the layer-by-layer electrostatic deposition of oppositively charged biopolymers onto the surfaces of emulsion droplets in combination with an enzymatic treatment was used to generate emulsions with different droplet interfaces to test this hypothesis ...201323978837
reducing the risk of foaming and decreasing viscosity by two-stage anaerobic digestion of sugar beet pressed pulp.anaerobic digestion (ad) of sugar beet pressed pulp (sbpp) is a promising treatment concept. it produces biogas as a renewable energy source making sugar production more energy efficient and it turns sbpp from a residue into a valuable resource. in this study one- and two-stage mono fermentation at mesophilic conditions in a continuous stirred tank reactor were compared. also the optimal incubation temperature for the pre-acidification stage was studied. the fastest pre-acidification, with a hyd ...201423963569
effect of sowing dates and vernalization on beta vulgaris l. cv. univers c-leaf structure.this research was conducted to study the effect of three different sowing dates (15th october, 15th november and 15th december) and two vernalization treatments (5 °c and -20 °c) on leaf structure of beta vulgaris l. cv. univers. the obtained data are summarized as follows: the maximum values of the most studied parameters; lower epidermis + spongy tissue thickness, midrib, mesophyll tissue, vascular bundle, collenchymatous tissue and number of xylem vessels per arm were found as a result of 15t ...201123961134
characterization of a β-1,3-glucan recognition protein from the beet armyworm, spodoptera exigua (insecta: lepidoptera: noctuidae).the β-1,3-glucan recognition protein gene from spodoptera exigua (seβgrp) was cloned and characterized. the cdna of this gene is 1 644 nucleotides in length and the predicted polypeptide is 491 amino acids (aa) in length, with a calculated molecular mass of 54.8 kda. the first 22 aa encode a predicted secretion signal peptide. a blast search, multiple sequence alignment, and phylogenetic analysis of the aa sequence of seβgrp revealed that this protein is most similar to the β-1,3-glucan recognit ...201323956146
foliar persistence and residual activity of methoxyfenozide against beet armyworm (lepidoptera: noctuidae).a greenhouse study was conducted to investigate the persistence of methoxyfenozide in pepper (capsicum annuum l.) foliage. an aqueous suspension of methoxyfenozide was sprayed on pepper plants at concentrations of 72 and 144 mg of active ingredient (a.i.)/l. foliage was collected at different intervals of time (0, 3, 5, 10, 20, 30, 40, and 50 days) after the treatment, and the methoxyfenozide residue was measured by high-performance liquid chromatography. the foliage was also used in bioassays t ...201323956070
acinetobacter kookii sp. nov., isolated from soil.two gram-stain-negative, non-fermentative bacterial strains, designated 11-0202(t) and 11-0607, were isolated from soil in south korea, and four others, luh 13522, luh 8638, luh 10268 and luh 10288, were isolated from a beet field in germany, soil in the netherlands, and sediment of integrated fish farms in malaysia and thailand, respectively. based on 16s rrna, rpob and gyrb gene sequences, they are considered to represent a novel species of the genus acinetobacter. their 16s rrna gene sequence ...201323950148
rna interference of glycerol biosynthesis suppresses rapid cold hardening of the beet armyworm, spodoptera exigua.the beet armyworm, spodoptera exigua, is a freeze-susceptible species that overwinters in temperate zones without diapause. a rapid cold hardening (rch) and supercooling capacity usually play crucial roles in survival during the overwintering period. this study identified a cryoprotectant as a rch factor of s. exigua. pre-exposure of s. exigua larvae to 4°c significantly increased survival at -10°c in all developmental stages from egg to adult. rch was dependent on the duration of the pre-exposu ...201323948473
changes in feed intake, nutrient digestion, plasma metabolites, and oxidative stress parameters in dairy cows with subacute ruminal acidosis and its regulation with pelleted beet pulp.the objectives of this study were to 1) determine the variation of nutrient digestion, plasma metabolites and oxidative stress parameters triggered by induced subacute ruminal acidosis (sara); and 2) evaluate the ability of pelleted beet pulp (bp) as a replacement for ground corn to alleviate sara. eight holstein-friesian cows were fed four diets during four successive17-day periods: 1) total mixed ration (tmr) containing 0% finely ground wheat (fgw) (w0); 2) tmr containing 10% fgw (w10); 3) tmr ...201323947764
occurrence of deoxynivalenol in wheat in slovakia during 2010 and 2011.in this study, a total of 299 grain samples of wheat were collected from four production regions: the maize, sugar beet, potato and feed sectors of slovakia. the samples were analyzed for deoxynivalenol (don) content by using an enzyme-linked immunosorbent assay ridascreen® fast don. analysis of variance revealed a significant difference between years in don contents (p < 0.027). the occurrence of samples with don was 82.2% in 2010, with maximum don content of 7.88 mg kg⁻¹, and 70.7% in 2011, wi ...201323917334
effect of supplementation on the performance of grazing belgian blue double-muscled heifers.six experiments were conducted to investigate the effect of a feed supplement on the performance of grazing belgian blue double-muscled (bbdm) heifers with an initial weight and age of 195 ± 43 kg and 190 ± 52 days. treatments included were: exp. 1: supplementation with beet pulp (bp): 2 kg/day per head v. ad libitum intake; exp. 2: supplementation ad libitum with bp v. a mixture of bp and soybean meal (sbm; bp/sbm ratio of 80/20; fw (fresh weight) basis); exp. 3: supplementation with 4 kg/day p ...201323915459
characterization of burdock mottle virus, a novel member of the genus benyvirus, and the identification of benyvirus-related sequences in the plant and insect genomes.the complete nucleotide sequence of the burdock mottle virus (bdmov) isolated from an edible burdock plant (arctium lappa) in japan has been determined. bdmov has a bipartite genome, whose organization is similar to rna1 and rna2 of benyviruses, beet necrotic yellow vein virus (bnyvv), beet soil-borne mosaic virus (bsbmv), and rice stripe necrosis virus (rsnv). bdmov rna1 (7038 nt) contains a single open reading frame (orf) encoding a 249-kda polypeptide that consists of methyl-transferase, heli ...201323911632
iron deficiency in plants: an insight from proteomic approaches.iron (fe) deficiency chlorosis is a major nutritional disorder for crops growing in calcareous soils, and causes decreases in vegetative growth as well as marked yield and quality losses. with the advances in mass spectrometry techniques, a substantial body of knowledge has arisen on the changes in the protein profiles of different plant parts and compartments as a result of fe deficiency. changes in the protein profile of thylakoids from several species have been investigated using gel-based tw ...201323898336
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