Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| probing the ser-ser-lys catalytic triad mechanism of peptide amidase: computational studies of the ground state, transition state, and intermediate. | peptide amidase (pam), a hydrolytic enzyme that belongs to the amidase signature (as) family, selectively catalyzes the hydrolysis of the c-terminal amide bond (co-nh(2)) of peptides. the recent availability of the x-ray structures of pam, fatty acid amide hydrolase, and malonamidase e2 has led to the proposal of a novel ser-ser-lys catalytic triad mechanism for the amide hydrolysis by the as enzymes. the molecular dynamics (md) simulations using the charmm force field were performed to explore ... | 2004 | 15595822 |
| bulgecin a: a novel inhibitor of binuclear metallo-beta-lactamases. | bulgecin a, a sulphonated n-acetyl-d-glucosamine unit linked to a 4-hydroxy-5-hydroxymethylproline ring by a beta-glycosidic linkage, is a novel type of inhibitor for binuclear metallo-beta-lactamases. using steady-state kinetic analysis with nitrocefin as the beta-lactam substrate, bulgecin a competitively inhibited the metallo-beta-lactamase bceii from bacillus cereus in its two-zinc form, but failed to inhibit when the enzyme was in the single-zinc form. the competitive inhibition was restore ... | 2005 | 15569001 |
| multiresistant stenotrophomonas maltophilia tunneled cvc-related sepsis, treated with systemic and lock therapy. | in the last decade, a remarkable increase in the incidence of nosocomial gram-negative infections has been observed. these pathogens represent a substantial problem in clinical practice, due to the high resistance profile of most commonly used antibiotics. this phenomenon is surely a co-factor that exposes these susceptible patients to infections caused by selected pathogens like multiresistant gram-negative rods. a typical example is represented by vap (ventilator-associated pneumonia) sustaine ... | 2004 | 15565919 |
| inhibition of the reattachment of young adult zebra mussels by single-species biofilms and associated exopolymers. | to determine the effects of single-species bacterial films and their associated extracellular products on the reattachment of young adult zebra mussels. | 2004 | 15546415 |
| pseudoxanthomonas mexicana sp. nov. and pseudoxanthomonas japonensis sp. nov., isolated from diverse environments, and emended descriptions of the genus pseudoxanthomonas finkmann et al. 2000 and of its type species. | three mesophilic bacteria (strains amx 26b(t), ur374_02 and 12-3(t)) isolated respectively from an anaerobic digester, human urine and urban riverside soil were characterized. cells were gram-negative, motile, non-sporulating, straight to curved rods with one polar flagellum and had a strictly respiratory metabolism with o(2) as the preferential terminal electron acceptor. phylogenetic analysis based on 16s rrna gene sequences revealed that all strains clustered within the xanthomonadaceae branc ... | 2004 | 15545466 |
| interactions in biofilms between listeria monocytogenes and resident microorganisms from food industry premises. | twenty nine bacterial strains were grown as binary culture biofilms with listeria monocytogenes to assess their influence on the settlement of the latter on stainless steel coupons. most of the strains had been isolated from food processing plants after cleaning and disinfection and were tentatively identified by the apilab plus 3.3.3 database (biomerieux). sixteen of them decreased l. monocytogenes biofilm colony forming units (cfu) counts. three strains, bacillus sp. ccl 9 an unidentified gram ... | 2004 | 15541798 |
| bacterium-based no2- biosensor for environmental applications. | a sensitive no2- biosensor that is based on bacterial reduction of no2- to n2o and subsequent detection of the n2o by a built-in electrochemical n2o sensor was developed. four different denitrifying organisms lacking no3- reductase activity were assessed for use in the biosensor. the relevant physiological aspects examined included denitrifying characteristics, growth rate, no2- tolerance, and temperature and salinity effects on the growth rate. two organisms were successfully used in the biosen ... | 2004 | 15528518 |
| in vitro antimicrobial activity of gatifloxacin compared with other quinolones against clinical isolates from cancer patients. | owing to the predominance of gram-positive pathogens in neutropenic cancer patients, newer generation quinolones with an expanded gram-positive spectrum and enhanced potency, may have a role to play for prophylaxis and/or empiric therapy in such patients. the in vitro activity of gatifloxacin was compared with that of ciprofloxacin, levofloxacin and trovafloxacin against 848 recent clinical isolates from cancer patients. against gram-positive organisms, gatifloxacin was the most active agent tes ... | 2004 | 15523180 |
| topoisomerases mutations and fluoroquinolone resistance in stenotrophomonas maltophilia. | 2004 | 15519490 | |
| evaluation of three molecular typing techniques for nonfermentative gram-negative bacilli. | to evaluate three different dna techniques for typing nonfermentative gram-negative bacilli isolated from latin american hospitals. | 2004 | 15518027 |
| association between stenotrophomonas maltophilia and lung function in cystic fibrosis. | stenotrophomonas maltophilia (sm) is a gram-negative non-fermenting bacteria cultured from the sputum of patients with cystic fibrosis (cf). to date, no information is available regarding the effect of this organism on lung function in cf. | 2004 | 15516471 |
| pitfalls of polymyxin antimicrobial susceptibility testing of pseudomonas aeruginosa isolated from cystic fibrosis patients. | with their potent activity against gram-negative bacteria, the polymyxins are important alternative antibiotics for cystic fibrosis (cf) patients. a retrospective evaluation of polymyxin activity against 6001 pseudomonas aeruginosa, 150 achromobacter xylosoxidans and 506 stenotrophomonas maltophilia cf isolates was initiated. in addition, we looked at how polymyxin susceptibility testing was affected by the testing method (agar dilution versus microdilution), the agent (polymyxin e versus polymy ... | 2004 | 15509615 |
| aggregation-based cooperation during bacterial aerobic degradation of polyethoxylated nonylphenols. | three bacterial strains were isolated from activated sludge samples of two treatment plants receiving domestic and industrial wastewaters containing polyethoxylated nonylphenols. one strain (va160) was isolated on rich medium, and the other two (bcal1 and bcal2) on mineral medium containing two industrial mixtures of nonylphenol ethoxylates as the sole carbon source. strain va160 was a gram-positive, spore forming, filamentous bacterium, producing aggregates during growth in liquid medium. on th ... | 2004 | 15501654 |
| analysis of tnt (2,4,6-trinitrotoluene)-inducible cellular responses and stress shock proteome in stenotrophomonas sp. ok-5. | in this study, the cellular responses of stenotrophomonas sp. ok-5 to explosive 2,4,6-trinitrotoluene (tnt) have been extensively analyzed. the stress shock proteins, which might contribute to enhancing cellular resistance to tnt-mediated toxicity, were induced at different concentrations of tnt used as a substrate for cell culture of stenotrophomonas sp. ok-5 capable of utilizing tnt. proteomic analysis for 2-de of soluble protein fractions from the culture of ok-5 exposed to tnt demonstrated a ... | 2004 | 15486709 |
| antibiograms of resistant gram-negative bacteria from scottish cf patients. | over a 19-month pilot phase, 93 multiply resistant gram-negative isolates from scottish cystic fibrosis patients were sent to a referral laboratory for further investigation. | 2004 | 15463901 |
| beta-lactamase inhibitors: evolving compounds for evolving resistance targets. | the many and diverse beta-lactamases produced by bacteria, particularly by gram-negative pathogens, are increasingly posing a serious threat to the clinical utility of beta-lactams. first-generation inhibitors (clavulanic acid, sulbactam, tazobactam) focus on ambler class a enzymes. however, recent structural upgrades of class a beta-lactamases (e.g. tem, shv) have extended their spectrum (extended-spectrum beta-lactamases and carbapenemases [sme, nmc-a, imi-1]) and have brought about the possib ... | 2004 | 15461559 |
| stenotrophomonas maltophilia as a nosocomial pathogen. | the aim of this study was to investigate the molecular epidemiology of stenotrophomonas maltophilia in a university hospital in turkey. thirty nine clinical isolates were collected from 37 patients and one from an environmental source between 1998 and 2001. susceptibility to 11 antimicrobials was studied. the isolates were categorised into six groups: a through f. trimethoprim sulfamethoxazole was the most active agent against the tested isolates. genotypic analysis by pulsed-field gel electroph ... | 2004 | 15460530 |
| nosocomial stenotrophomonas maltophilia cross-infection: three cases in newborns. | increased nosocomial stenotrophomonas maltophilia infection rates in newborns, especially in recent years, are a significant cause for concern. these cases are the second case group in the literature to have been identified as nosocomial cross-infection with s. maltophilia in neonates. | 2004 | 15454897 |
| [tricuspid infective endocarditis caused by stenotrophomonas maltophilia in a patient without risk factors]. | 2004 | 15388041 | |
| inactivation of macrolides by producers and pathogens. | inactivation, one of the mechanisms of resistance to macrolide, lincosamide and streptogramin (mls) antibiotics, appears to be fairly rare in clinical isolates in comparison with target site modification or efflux. however, inactivation is one of the major mechanisms through which macrolide-producing organisms avoid self-damage during antibiotic biosynthesis. the inactivation mechanisms for mls antibiotics in pathogens are mainly hydrolysis, phosphorylation, glycosylation, reduction, deacylation ... | 2004 | 15379733 |
| improved laboratory diagnosis of bacterial and fungal infections in patients with hematological malignancies using pcr and ribosomal rna sequence analysis. | during october 1999 to november 2000, 98 blood culture specimens from the same number of febrile episodes originating from 49 patients with hematological malignancies were examined for the presence of eubacteria and fungi based on 16s rrna gene and the 5.8, 18 and 28s rrna combined with in vitro pcr amplification and sequencing, in addition to conventional blood culture laboratory techniques. nineteen of the samples were associated with positive blood cultures. eubacterial (16s rrna) pcr detecte ... | 2004 | 15370218 |
| stenotrophomonas maltophilia infection in patients receiving continuous ambulatory peritoneal dialysis. | stenotrophomonas maltophilia is a gram-negative bacillus that has become increasingly recognized as an important nosocomial pathogen, particularly in individuals with severe debilitation or immunosuppression. s. maltophilia is also characterized by its resistance to multiple antibiotics. s maltophilia peritonitis in capd (continuous ambulatory peritoneal dialysis) patients is associated with a poor prognosis and loss of capd catheter. no report concerning this entity has been presented in korea. ... | 2004 | 15366641 |
| lethal pulmonary hemorrhage caused by a fulminant stenotrophomonas maltophilia respiratory infection in an acute myeloid leukemia patient. | stenotrophomonas maltophilia (sm) pneumonia in immunocompromized hosts is an increasingly common nosocomial infection. even though resistant to multiple antimicrobials, this gram-negative bacteria usually does not present with a fulminant course leading to a fatal hemorrhagic respiratory infection in neutropenic patients. we report here the case of a 63-year-old woman treated by intensive chemotherapy for acute myeloid leukemia (aml) who presented while severely neutropenic and thrombocytopenic ... | 2004 | 15360016 |
| microbiological identification in cystic fibrosis patients with cftr i1234v mutation. | recurrent and chronic bacterial pulmonary infection is the major cause of morbidity and mortality in cystic fibrosis (cf). over 6 months, 72 sputa or oropharyngeal samples were examined from 36 arab bedouin cf patients attending hamad general hospital, doha, qatar. more than 100 pathogens were isolated, mostly haemophilus influenzae, staphylococcus aureus and pseudomonas aeruginosa. unusual pathogens included stenotrophomonas maltophilia, acaligenes xylosoxidans and mycobacterium abscessus. it i ... | 2004 | 15357563 |
| central venous catheter-related bacteremia due to gram-negative bacilli: significance of catheter removal in preventing relapse. | to study the characteristics of catheter-related, gram-negative bacteremia (gnb) and the role of central venous catheter (cvc) removal. | 2004 | 15357155 |
| increase in methicillin-resistant staphylococcus aureus acquisition rate and change in pathogen pattern associated with an outbreak of severe acute respiratory syndrome. | an outbreak of severe acute respiratory syndrome (sars) occurred in our 22-bed intensive care unit (icu; prince of wales hospital, hong kong, hksar, china) from 12 march to 31 may 2003, when only patients with sars were admitted. this period was characterized by the upgrading of infection control precautions, which included the wearing of gloves and gowns all the time, an extensive use of steroids, and a change in antibiotic prescribing practices. the pattern of endemic pathogenic organisms, the ... | 2004 | 15356814 |
| stenotrophomonas maltophilia peritonitis in capd patients: susceptibility to antibiotics and treatment outcome: a report of five cases. | 2004 | 15335157 | |
| bacterial communities and enzyme activities of pahs polluted soils. | three soils (i.e. a belgian soil, b-bt, a german soil, g, and an italian agricultural soil, i-bt) with different properties and hydrocarbon-pollution history with regard to their potential to degrade phenanthrene were investigated. a chemical and microbiological evaluation of soils was done using measurements of routine chemical properties, bacterial counts and several enzyme activities. the three soils showed different levels of polycyclic aromatic hydrocarbons (pahs), being their contamination ... | 2004 | 15331267 |
| community-acquired infection due to stenotrophomonas maltophilia: a rare cause of meningitis. | 2004 | 15325602 | |
| relationship between oral motor dysfunction and oral bacteria in bedridden elderly. | the purpose of this study was to analyze the relationship between oral bacterial colonization and oral motor dysfunction. | 2004 | 15316545 |
| [epidemiology and drug resistance of the pathogenic microbes in the complicated infection of hematological malignancies]. | to investigate the epidemiological characteristics and drug resistance profile of the infection in patients with hematological malignancies. | 2004 | 15308008 |
| [molecular epidemiology of stenotrophomonas maltophilia strains isolated from paediatric patients]. | as alternative regimens in therapy of stenotrophomonas maltophilia infections are limited, epidemiological investigation of s. maltophilia is necessary for developing new strategies in prophylaxis of these infections. "enterobacterial repetetive intergenic consensus-polymerase chain reaction (eric-pcr)", is a simple and rapid method to detect the genomic polymorphism at the strain level in nosocomial outbreaks due to s. maltophilia. the aim of this study was to investigate the molecular epidemio ... | 2004 | 15293897 |
| toys in a pediatric hospital: are they a bacterial source? | in children's hospitals, children are commonly provided with toys. measures to guarantee the safety of these toys are usually not taken. this study was conducted to determine whether toys were contaminated with potentially pathogenic bacteria when they arrived in the hospital, and whether they were contaminated in the hospital. | 2004 | 15292894 |
| 16s rdna pcr analysis of infectious keratitis: a case series. | to discuss the value of polymerase chain reaction (pcr) in the management of bacterial infectious keratitis. | 2004 | 15291943 |
| stenotrophomonas maltophilia bacteremia. | 2004 | 15287393 | |
| three cases of post-traumatic endophthalmitis caused by unusual bacteria. | three cases of post-traumatic endophthalmitis caused by unusual bacteria are presented. the pathogens identified were: (i) bacillus cereus and citrobacter freundii; (ii) pseudomonas fluorescens; and (iii) chryseobacterium meningosepticum and stenotrophomonas maltophilia. two of these pathogens have not previously been reported to cause endophthalmitis. the available literature regarding the individual cases is summarized and a brief discussion of post-traumatic endophthalmitis is presented, with ... | 2004 | 15281988 |
| stenotrophomonas africana drancourt et al. 1997 is a later synonym of stenotrophomonas maltophilia (hugh 1981) palleroni and bradbury 1993. | type and reference strains of stenotrophomonas maltophilia and stenotrophomonas africana were compared with each other and with the type strains of other stenotrophomonas species, using sds-page of whole-cell proteins, dna-dna hybridization and extensive biochemical characterization. s. maltophilia lmg 958t and s. africana lmg 22072t had very similar whole-cell-protein patterns and were also biochemically very similar. a dna-dna binding level of 70% between both type strains confirmed that s. af ... | 2004 | 15280297 |
| probing the dynamics of a mobile loop above the active site of l1, a metallo-beta-lactamase from stenotrophomonas maltophilia, via site-directed mutagenesis and stopped-flow fluorescence spectroscopy. | a structural feature shared by the metallo-beta-lactamases is a flexible loop of amino acids that extends over their active sites and that has been proposed to move during the catalytic cycle of the enzymes, clamping down on substrate. to probe the movement of this loop (residues 152-164), a site-directed mutant of metallo-beta-lactamase l1 was engineered that contained a trp residue on the loop to serve as a fluorescent probe. it was necessary first, however, to evaluate the contribution of eac ... | 2004 | 15271998 |
| analysis of sequence variation among smedef multi drug efflux pump genes and flanking dna from defined 16s rrna subgroups of clinical stenotrophomonas maltophilia isolates. | to determine the level of variation in the smedef efflux pump and smet transcriptional regulator genes among three defined 16s rrna sequence subgroups of clinical stenotrophomonas maltophilia isolates. | 2004 | 15254029 |
| physiological characterization of mycobacterium sp. strain 1b isolated from a bacterial culture able to degrade high-molecular-weight polycyclic aromatic hydrocarbons. | the aim of this study was to further characterize a bacterial culture (vun 10,010) capable of benzo[a]pyrene cometabolism. | 2004 | 15239690 |
| in vivo folding of recombinant metallo-beta-lactamase l1 requires the presence of zn(ii). | metallo-beta-lactamase l1, secreted by pathogenic stenotrophomonas maltophilia, is a dinuclear zn(ii)-containing enzyme that hydrolyzes almost all known penicillins, cephalosporins, and carbapenems. the presence of zn(ii) ions in both metal binding sites is essential for full enzymatic activity; however, the mechanism of physiological metal incorporation is unknown. to probe metal incorporation, l1 was over-expressed in minimal media with (mml1+zn) and without (mml1-zn) zn(ii) added to the media ... | 2004 | 15238636 |
| [multiresistant nosocomial bacterial strains and their "in vitro" susceptibility to chloramphenicol and colistin] | objective: a multicenter study was conducted to obtain "in vitro" chloramphenicol and colistin susceptibility data on multiresistant hospital bacterial pathogens in slovak republic. material and methods: during the period of april-june 2001, 628 clinical bacterial multiresistant isolates from patients with serious infections were selected in 10 hospitals and tested to a large scale of antibiotics by means of a microdilution method. the strains expressed either a significant resistance phenotype ... | 2004 | 15227603 |
| spontaneous biliopneumothorax (thoracobilia) following gastropleural fistula due to stomach perforation by nasogastric tube. | gastropleural fistula may occur after pulmonary resection, perforated paraesophageal hernia, perforated malignant gastric ulcer at the fundus, or gastric bypass surgery for morbid obesity. we describe a case of gastropleural fistula after stomach perforation by a nasogastric tube in a patient who underwent billroth ii gastric resection for adenocarcinoma. left biliopneumothorax occurred and was treated by thoracic drainage with -20 cm h2o aspiration. as gastropleural fistula persisted, laparotom ... | 2004 | 15223464 |
| studies on cultured and uncultured microbiota of wild culex quinquefasciatus mosquito midgut based on 16s ribosomal rna gene analysis. | to describe the midgut microbial diversity and the candidate bacteria for the genetic manipulation for the generation of transgenic mosquitoes refractory to transmission of diseases, the microbiota of wild culex quinquefasciatus mosquito midgut was studied using a conventional culture technique and analysis of a 16s ribosomal rna (rrna) gene sequence library. the culturable microbiota was identified as acinetobacter junii, ac. calcoaceticus, aeromonas culicicola, bacillus thuringiensis, microbac ... | 2004 | 15210998 |
| cytokine stimulation by pseudomonas aeruginosa--strain variation and modulation by pulmonary surfactant. | pulmonary surfactant and its components are part of the first-line immune defense within the lung. here the authors show that the surfactant protein (sp) sp-d, but not sp-a, agglutinates some clinical isolates of pseudomonas aeruginosa and stenotrophomonas maltophilia. no agglutination of staphylococcus aureus or burkholderia cepacia was observed. the sp-d-induced agglutination of p. aeruginosa was not dependent on a specific lipopolysaccharide (lps) serotype. the authors also show that sp-d, bu ... | 2004 | 15195551 |
| doripenem (s-4661), a novel carbapenem: comparative activity against contemporary pathogens including bactericidal action and preliminary in vitro methods evaluations. | to investigate the potency of doripenem, a broad-spectrum carbapenem characterized by a wider spectrum of activity combining antimicrobial and bactericidal features of imipenem and meropenem. | 2004 | 15190031 |
| culture-independent analysis of midgut microbiota in the arbovirus vector culicoides sonorensis (diptera: ceratopogonidae). | differences in midgut microbial communities inhabiting culicoides spp., insect vectors of virus pathogens, may affect the variation observed in the ability of these biting midges to propagate arthropod-borne viruses. as a first step toward addressing this hypothesis, midgut bacterial communities were compared between culicoides species expected to be efficient and inefficient vectors of virus pathogens. we used 16s rdna sequence and restriction fragment information to provisionally identify 36 b ... | 2004 | 15185934 |
| high phylogenetic diversity of transconjugants carrying plasmid pjp4 in an activated sludge-derived microbial community. | transconjugants of plasmid pjp4, originating from an agar plate mating of a pseudomonas putida donor with an activated sludge-derived microbial community, were isolated and identified by partial 16s rdna sequencing. the transconjugant strains belonged to a variety of genera of the alpha-, beta-, gamabeta- and gamma-classes of the proteobacteria, mostly to the families rhizobiaceae and comamonadaceae and the genus stenotrophomonas. only p. putida and delftia spp. strains were able to grow on 2,4- ... | 2004 | 15183866 |
| infection control and the significance of sputum and other respiratory secretions from adult patients with cystic fibrosis. | there is limited data available on the environmental and public health impact of the microbiological hazards associated with sputa from patients with cystic fibrosis [cf]. pseudomonas aeruginosa, burkholderia cenocepacia (formerly burkholderia cepacia genomovar iii), staphylococcus aureus and stenotrophomonas maltophilia are bacterial pathogens which are commonly found in the sputum of cf patients. a study was performed to ascertain the amount of sputum produced relating to microbial loading, as ... | 2004 | 15175107 |
| antimicrobial resistance among non-fermentative gram-negative bacilli isolated from the respiratory tracts of italian inpatients: a 3-year surveillance study by the italian epidemiological survey. | the italian epidemiological survey evaluated antibiotic susceptibility of non-fermentative gram-negative bacilli isolated from inpatient respiratory-tract specimens collected throughout italy during 1997-1999. the minimal inhibitory concentrations of 14 antibiotics for 1474 pseudomonas aeruginosa strains, 307 stenotrophomonas maltophilia strains and 114 acinetobacter baumannii strains were determined in 57 clinical microbiology laboratories by means of a standardised micro-dilution method. the m ... | 2004 | 15164966 |
| [isolation measurements for cystic fibrosis patients]. | respiratory tract infections significantly contribute to morbidity and mortality of cystic fibrosis patients. | 2004 | 15162255 |
| regulatory regions of smedef in stenotrophomonas maltophilia strains expressing different amounts of the multidrug efflux pump smedef. | the smet-smedef region and the smet gene, which encodes the smedef repressor, are highly polymorphic. few changes in smet might be associated with smedef overexpression. the results obtained with cellular extracts suggest that mutant smet proteins cannot bind to the operator and that other transcription factors besides smet are involved in the regulation of smedef expression. | 2004 | 15155232 |
| [off-pump coronary artery bypass via left thoracotomy in patient with infective pancreatic fistula after surgery for bile duct carcinoma; report of a case]. | a 62-year-old man with infective pancreatic fistula after surgery for bile duct carcinoma underwent off-pump coronary artery bypass (opcab) through left thoracotomy to avoid the use of cardiopulmonary bypass and the postoperative mediastinitis, since this patient has infective pancreatic fistula close to the xiphoid process. the coronary arterial revascularizations were performed: left internal thoracic artery to left anterior descending branch and saphenous vein graft to descending thoracic aor ... | 2004 | 15151045 |
| stenotrophomonas maltophilia: the significance and role as a nosocomial pathogen. | stenotrophomonas maltophilia is a significant pathogen that primarily affects patients with co-morbid illnesses, usually malignancies, who have been hospitalized for prolonged periods and received broad-spectrum antibiotic therapy. the organism has been associated with an expanding spectrum of clinical manifestations associated with high case to fatality ratios. therapy of these infections presents a significant challenge both for the clinician and the microbiologists because of its high-level a ... | 2004 | 15142709 |
| enrichment and identification of bacteria capable of reducing chemical oxygen demand of anaerobically treated molasses spent wash. | the aim of this study was to isolate and identify bacterial strains capable of using recalcitrant compounds of molasses spent wash as sole carbon source from the soils of abandoned sites of distillery effluent discharge and characterize their ability of reducing the chemical oxygen demand (cod) of the spent wash. | 2004 | 15139920 |
| an epidemiological analysis of stenotrophomonas maltophilia strains in a university hospital. | the aim of this investigation was to evaluate the epidemiology of stenotrophomonas maltophilia in a university hospital of turkey. from june 2000 to december 2001, s. maltophilia strains were collected, clinical presentations were noted, and mic determinations were performed by means of e-test. enterobacterial repetitive intergenic consensus sequences-pcr (eric-pcr) was used for molecular typing of the strains. forty-four strains of s. maltophilia were isolated from 41 hospitalized patients in a ... | 2004 | 15118205 |
| aerobic biotransformation of octylphenol polyethoxylate surfactant in soil microcosms. | the biotransformation of octylphenol polyethoxylate surfactant (triton x-100) and bacterial communities in soil microcosms was investigated. the soil microcosms were designed to simulate real sites of bioremediation. the soil used in this study was contaminated with pesticides and alkylphenol polyethoxylate surfactants over a long period of time. the nitrogen source, (nh4)2so4 and the mineral salt basal solution were added to the microcosm and the water content was adjusted to 50% with distilled ... | 2004 | 15116878 |
| the emergence of stenotrophomonas maltophilia as a significant nosocomial pathogen at the university hospital of the west indies. | stenotrophomonas maltophilia is emerging worldwide as a nosocomial pathogen. it is associated with certain risk factors and a wide range of infections. this study was done to document its emergence at the university hospital of the west indies and to determine the incidence, distribution and risk factors associated with it. a retrospective study was conducted over the period april 1997 to december 2000. clinical records were available for 46 of the 66 patients identified over the study period. f ... | 2004 | 15114888 |
| double-disk synergy test positivity in stenotrophomonas maltophilia clinical strains. | the double-disk synergy test (ddst) using mueller-hinton agar and antibiotic disks with centrally positioned disks of amoxicillin-clavulanate, ampicillin-sulbactam, and piperacillin-tazobactam and, at a center-to-center distance of 25-30 mm, 2-4 disks with 10 various beta-lactam antibiotics per one plate was performed in 58 clinical isolates of stenotrophomonas maltophilia to determine the effectivity of 3 beta-lactamase inhibitors. when tested with clavulanate as the central beta-lactamase inhi ... | 2004 | 15114869 |
| identification of pseudomonas aeruginosa, burkholderia cepacia complex, and stenotrophomonas maltophilia in respiratory samples from cystic fibrosis patients using multiplex pcr. | a multiplex pcr method was developed to identify p. aeruginosa, b. cepacia complex, and s. maltophilia directly in sputum and oropharyngeal samples from cf patients. one hundred and six patients (53 male, and 53 female) attending our pulmonology clinic were studied from september 2000-april 2001. two hundred and fifty-seven samples were cultured in selective media and submitted to multiplex pcr reactions, using three primer pairs targeting specific genomic sequences of each species, with an addi ... | 2004 | 15114555 |
| resistance of gram-negative non-fermentative bacilli causing bloodstream infection, vienna, 1996-2003. | 2004 | 15112063 | |
| phenotypic properties, drug susceptibility and genetic relatedness of stenotrophomonas maltophilia clinical strains from seven hospitals in rio de janeiro, brazil. | to investigate phenotypic aspects including biotyping, drug susceptibility and production of extracellular enzymes and genetic diversity of stenotrophomonas maltophilia clinical strains obtained from seven hospitals in rio de janeiro, brazil. | 2004 | 15078532 |
| bacterial diversity of the inner mongolian baer soda lake as revealed by 16s rrna gene sequence analyses. | bacterial diversity associated with baer soda lake in inner mongolia of china was investigated using a culture-independent method. bacterial 16s rrna gene libraries were generated using bacterial oligonucleotide primers, and 16s rrna gene sequences of 58 clones were analyzed phylogenetically. the library was dominated by 16s rdnas of gram-negative bacteria (24% alpha-proteobacteria, 31% beta-proteobacteria, 33% gamma-proteobacteria, and 2% delta-proteobacteria), with a lower percentage of clones ... | 2004 | 15064989 |
| algorithm for the identification of bacterial pathogens in positive blood cultures by real-time lightcycler polymerase chain reaction (pcr) with sequence-specific probes. | we developed real-time polymerase chain reaction (pcr) assays for rapid detection of the most common and clinically relevant bacteria in positive blood culture bottles, including staphylococcus spp., s. epidermidis, s. aureus, enterococcus spp. (including differentiation of e. faecalis and e. faecium), streptococcus spp., streptococcus agalactiae, enterobacteriaceae, e. coli, pseudomonas aeruginosa, stenotrophomonas maltophilia, acinetobacter spp., bacteroides spp., haemophilus influenzae, and n ... | 2004 | 15062914 |
| [investigation on the antibiotic resistance and risky factors of nosocomial infections caused by stenotrophomonas maltophilia]. | to investigate the antibiotic resistance and risky factors of nosocomial infections caused by stenotrophomonas maltophilia, so as to help elucidate the difference of drug resistance between metallic beta-lactamase (mbl) producing and non-mbl producing strains. | 2004 | 15059439 |
| production of n-acylhomoserine lactone signal molecules by gram-negative soil-borne and plant-associated bacteria. | quorum-sensing control mediated by n-acylhomoserine lactone (ahl) signal molecules has been established as a key feature in the regulation of various metabolic traits in many bacteria. approximately 300 strains representing 6 genera and 18 species of soil-borne and plant-associated gram-negative bacteria isolated in various regions of the former ussr using two reporter systems were screened for ahl production. the production was observed in 17.5% of the screened bacterial strains. positive respo ... | 2003 | 15058194 |
| isolated necrotizing epiglottitis: report of a case in a neutropenic patient and review of the literature. | isolated necrotizing epiglottitis (ine) is an unusual condition that may develop in immunocompromised patients. only 3 cases of ine have been reported in the english-language literature; this is the fourth case, one in a 27-year-old woman who had neutropenia due to cytomegalovirus infection. stenotrophomonas maltophilia and candida albicans were isolated from the culture of necrotic material. the features of ine are discussed here by reviewing the literature and by examining the characteristics ... | 2004 | 15053207 |
| identification of genomic groups in the genus stenotrophomonas using gyrb rflp analysis. | stenotrophomonas maltophilia isolates have been recovered from various clinical samples, including the respiratory tract of cystic fibrosis (cf) patients, but this organism is also widespread in nature. previously it has been shown that there is a considerable genomic diversity within s. maltophilia. the aims of our study were to determine the taxonomic resolution of restriction fragment length polymorphism (rflp) analysis of the polymerase chain reaction-amplified gyrb gene for the genus stenot ... | 2004 | 15039092 |
| [aspergillus flavus endocarditis on a native valve]. | a 33 year old female had febrile aplasia following a first chemotherapy treatment for acute t lymphoid leukaemia. she was transferred to intensive care for acute respiratory distress due to bilateral pneumonia with stenotrophomonas maltophilia septicaemic shock. after an initial improvement with antibiotic treatment, she developed multiple necrotic cutaneous lesions. a skin biopsy showed the presence of aspergillus flavus in large quantities in the vascular lumina. echocardiography revealed a vo ... | 2004 | 15032419 |
| antimicrobial susceptibility pattern of clinical isolates of non-fermentative bacteria. | 152 nonfermentative bacteria were isolated from a total number of 965 clinical samples processed routinely in the laboratory of microbiology department, m.k.c.g medical college in south orissa accounting to a prevalence rate of 15.75%. pseudomonas spp. (both pigmented and non-pigmented strains) were isolated in maximum percentage (73.6%) followed by acinetobacter spp. (19.7%) and alkaligenes faecalis (4.6%). rarely encountered species were eikenella corrodens (1.3%) and stenotrophomonas maltophi ... | 2003 | 15025332 |
| non-fermenters in human infections. | one thousand and one hundred thirty non-fermenting gram negative bacteria were isolated from various samples. of these, pseudomonas aeruginosa was the commonest isolate (72.83%) followed by acinetobacter anitratus (8.4%), alcaligenes faecalis (7.6%), acinetobacter lwoffi (4.4%), pseudomonas flourescens (2.4%), schwanella putrefaciens (1.6%), stenotrophomonas maltophilia (1.6%), pseudomonas putida (0.4%), bravundimonas vesicularis (0.4%) and flavobacterium meningosepticum (0.4%). antibiotic sensi ... | 2003 | 15022936 |
| [nationwide sensitivity surveillance of ciprofloxacin and various parenteral antibiotics against bacteria isolated from patients with severe infections--the first ciproxan iv special investigation in 2001]. | the parenteral injection of ciprofloxacin (cpfx), a fluoroquinolone antimicrobial drug, was approved in september 2000 and a re-examination period of 6 years was set at that time. as a special investigation to apply for re-examination of this drug, it has been planned to conduct 3 nationwide surveillances during the re-examination period by collecting clinically isolated bacteria from patients with severe infections, to whom the drug was mainly indicated, and examining drug susceptibilities of t ... | 2003 | 15007872 |
| high antibiotic susceptibility among bacterial pathogens in swedish icus. report from a nation-wide surveillance program using ta90 as a novel index of susceptibility. | local infection control measures, antibiotic consumption and patient demographics from 1999-2000 together with bacteriological analyses were investigated in 29 icus participating in the icu-strama programme. the median antibiotic consumption per icu was 1147 (range 605-2143) daily doses per 1000 occupied bed d (ddd1000). antibiotics to which > 90% of isolates of an organism were susceptible were defined as treatment alternatives (ta90). the mean number of ta90 was low (1-2 per organism) for ente ... | 2004 | 15000555 |
| the combination of aztreonam and cefozopran against stenotrophomonas maltophilia. | aztreonam is suited for combination chemotherapy because it could be a potent beta-lactamase inhibitor. we designed a study to show the inhibitory activity of aztreonam, using stenotrophomonas maltophilia, which produces both carbapenemase l1 and penicillinase l2. aztreonam showed considerable synergy with cefpirome and contributed to a decrease in minimum inhibitory concentrations of cefozopran. in further examinations, the mean viable bacterial counts in cultures treated with aztreonam-cefozop ... | 2004 | 14991523 |
| activities of ciprofloxacin and moxifloxacin against stenotrophomonas maltophilia and emergence of resistant mutants in an in vitro pharmacokinetic-pharmacodynamic model. | a two-compartment in vitro pharmacokinetic-pharmacodynamic model, with full computer-controlled devices, was used to accurately simulate human plasma pharmacokinetic profiles after multidose oral regimens of ciprofloxacin (750 mg every 12 h) and moxifloxacin (400 mg every 24 h) during 48 h. pharmacodynamics of these drugs was investigated against three quinolone-susceptible strains of stenotrophomonas maltophilia (mics of ciprofloxacin and moxifloxacin of 0.5 to 2 and 0.0625 to 0.5 microg/ml, re ... | 2004 | 14982788 |
| quantitative evaluation of the therapeutic effects of antibiotics using silkworms infected with human pathogenic microorganisms. | the injection of bacteria (staphylococcus aureus, stenotrophomonas maltophilia) or true fungi (candida albicans, candida tropicalis) that are pathogenic to humans into the silkworm hemolymph leads to death of the larvae within 2 days. antibiotics used for clinical purposes have therapeutic effects on silkworms infected with these pathogens. the 50% effective doses obtained by injection into the silkworm hemolymph are consistent with those reported for mice. injection of vancomycin and kanamycin ... | 2004 | 14982763 |
| [surveillance on antibiotic resistance of stenotrophomonas maltophilia in chengdu and chongqing area]. | to survey the antibiotic resistance of stenotrophomonas maltophilia in chengdu and chongqing area and guide the rational antibiotics usage in the treatment of stenotrophomonas maltophilia infection. | 2004 | 14981826 |
| microbiology of early cf lung disease. | recent bronchoscopy studies using assays to measure inflammation and molecular typing techniques have facilitated an increased understanding of the early events that occur within the lungs of young children with cystic fibrosis and provided additional insights into the natural history of lung disease in children. in 2000, the us cf national patient registry data showed that among 1000 infants <2 years of age, the first bacterial pathogens detected are haemophilus influenzae, staphylococcus aureu ... | 2004 | 14980298 |
| in vitro susceptibility of stenotrophomonas maltophilia isolates: comparison of disc diffusion, etest and agar dilution methods. | the disc diffusion, etest and agar dilution techniques were compared to evaluate the antimicrobial susceptibility profile of 70 stenotrophomonas maltophilia isolates to seven antimicrobial agents. the s. maltophilia isolates were consecutively collected from may 2000 to may 2002 from individual patients, who were hospitalized in a private brazilian hospital. the antimicrobial susceptibility tests were carried out and interpreted according to the national committee for clinical laboratory standar ... | 2004 | 14973153 |
| [corneal infiltrates and ulcers. a retrospective study of 239 eyes]. | in our outpatient service the number of corneal infiltrates and ulcers associated with contact lens wear in young patients increased during the last years. therefore we evaluated all patients with keratitis regarding to the reason. | 2004 | 14872265 |
| microbial transformation of pyrethroid insecticides in aqueous and sediment phases. | recent studies showed that synthetic pyrethroids (sps) can move via surface runoff into aquatic systems. fifty-six of sp-degrading bacteria strains were isolated from contaminated sediments, of which six were evaluated for their ability to transform bifenthrin and permethrin in the aqueous phase and bifenthrin in the sediment phase. in the aqueous phase, bifenthrin was rapidly degraded by strains of stenotrophomonas acidaminiphila, and the half-life (t1/2) was reduced from >700 h to 30 to 131 h. ... | 2004 | 14768859 |
| high genetic diversity among stenotrophomonas maltophilia strains despite their originating at a single hospital. | the levels of genetic relatedness of 139 stenotrophomonas maltophilia strains recovered from 105 hospitalized non-cystic fibrosis patients (51% from medical wards, 35% from intensive care units, and 14% from surgical wards) and 7 environmental sources in the same hospital setting during a 4-year period were typed by the pulsed-field gel electrophoresis (pfge) technique. a total of 99 well-defined distinct xbai pfge patterns were identified (simpson's discrimination index, 0.996). the dendrogram ... | 2004 | 14766838 |
| synthesis, and antiprotozoal and antibacterial activities of s-substituted 4,6-dibromo- and 4,6-dichloro-2-mercaptobenzimidazoles. | the synthesis and some germicidal activities in vitro of two congener series of s-substituted 4,6-dihalogeno-2-mercapto-1h-benzimidazoles are reported. there was no substantial difference between antibacterial activities of corresponding 4,6-dichloro- and 4,6-dibromo-derivatives. the present results confirm lower susceptibility to substituted benzimidazoles of gram-negative compared to gram-positive bacteria. minimum inhibitory concentrations (mics) of a majority of the novel derivatives ranged ... | 2004 | 14757505 |
| sphingosine-1-phosphate induces proliferation and morphological changes of neural progenitor cells. | sphingosine-1-phosphate (s1p) is a lipid mediator that exerts multiple cellular functions through activation of g-protein-coupled receptors. although the role of s1p on angiogenesis is well established, its role in neurogenesis is unknown. we examined the effects of s1p on g-protein activation in brain sections of rat embryo and on neural progenitor cells in culture. intense s1p-stimulated [35s]gtpgammas labeling was observed as early as e15 in the neuroepithelium and differentiating fields thro ... | 2004 | 14756825 |
| biodegradation of dna and nucleotides to nucleosides and free bases. | thirty-two different microorganisms were examined in order to check their ability to degrade an exogenous dna. bacteria from species: stenotrophomonas maltophilia, brevundimonas diminuta, bacillus subtilis, mycobacterium butyricum and fungus fusarium moniliforme were capable to degrade dna to nucleic bases or their derivatives. degradation of dna by s. maltophilia resulted in formation of free bases, such as hypoxanthine, thymine, uracil and xanthine. the optimum concentration of dna seemed to b ... | 2004 | 14751311 |
| contribution of integrons, and smeabc and smedef efflux pumps to multidrug resistance in clinical isolates of stenotrophomonas maltophilia. | the contribution of integrons and efflux pumps to multidrug resistance in stenotrophomonas maltophilia was evaluated. | 2004 | 14749340 |
| class 1 integrons increase trimethoprim-sulfamethoxazole mics against epidemiologically unrelated stenotrophomonas maltophilia isolates. | twenty-five plasmid-specified antimicrobial resistance determinants common to gram-negative bacilli from nosocomial infection were investigated from 31 stenotrophomonas maltophilia isolates. twenty-four clones were identified by pulsed-field gel electrophoresis, and in three clones that exhibited an increased trimethoprim-sulfamethoxazole mic, the sul1 determinant was found. these results support not only the higher spread of class 1 integrons compared to other mechanisms but also the potential ... | 2004 | 14742234 |
| overexpression of the multidrug efflux pump smedef impairs stenotrophomonas maltophilia physiology. | the use of antibiotics for the treatment of infectious diseases has led to important changes in the structure of pathogenic bacterial populations. however, these changes could be buffered if the expression of antibiotic resistance genes were to lead to the counter-selection of antibiotic-resistant strains in antibiotic-free environments. to test the effect of antibiotic resistance on bacterial fitness, we analysed the effect of the overproduction of the multidrug efflux pump smedef on the physio ... | 2004 | 14739147 |
| case-control study of stenotrophomonas maltophilia acquisition in cystic fibrosis patients. | the aims of this case-control study were to describe the characteristics of cystic fibrosis (cf) patients who isolated stenotrophomonas maltophilia in sputum, to determine risk factors for acquisition, to assess persistence of the organism and clinical outcomes postacquisition. data were collected from 1991-1999. cf patients and controls (who had never isolated s. maltophilia) were matched for age (+/- 1 yr), sex and forced expiratory volume in one second (+/- 10%). data were collected from the ... | 2004 | 14738239 |
| implantable venous port-related infections in cancer patients. | to evaluate the characteristics of implantable venous port-related infections in patients with malignancies. | 2004 | 14727170 |
| antibiotic treatment of multidrug-resistant organisms in cystic fibrosis. | respiratory tract infection with eventual respiratory failure is the major cause of morbidity and mortality in cystic fibrosis (cf). infective exacerbations need to be treated promptly and effectively to minimize potentially accelerated attrition of lung function. the choice of antibiotic depends on in vitro sensitivity patterns. however, physicians treating patients with cf are increasingly faced with infection with multidrug-resistant isolates of pseudomonas aeruginosa. in addition, innately r ... | 2003 | 14719998 |
| efflux-mediated multiresistance in gram-negative bacteria. | multiresistance in gram-negative pathogens, particularly pseudomonas aeruginosa, stenotrophomonas maltophilia, acinetobacter spp. and the enterobacteriaceae, is a significant problem in medicine today. while multiple mechanisms often contribute to multiresistance, a broadly distributed family of three-component multidrug efflux systems is an increasingly recognised determinant of both intrinsic and acquired multiresistance in these organisms. homologues of these efflux systems are also readily i ... | 2004 | 14706082 |
| antimicrobial susceptibility and synergy studies of stenotrophomonas maltophilia isolates from patients with cystic fibrosis. | stenotrophomonas maltophilia is a newly emerging pathogen being detected with increasing frequency in patients with cystic fibrosis (cf). the impact of this multidrug-resistant organism on lung function is uncertain. the optimal treatment for s. maltophilia in cf patients is unknown. we studied the in vitro activity of ten antimicrobial agents, and conducted synergy studies by using checkerboard dilutions of eight pairs of antimicrobial agents against strains isolated from 673 cf patients from 1 ... | 2004 | 14693535 |
| biofilm formation by stenotrophomonas maltophilia: modulation by quinolones, trimethoprim-sulfamethoxazole, and ceftazidime. | we investigated the in vitro effects of seven fluoroquinolones (ciprofloxacin, grepafloxacin, levofloxacin, moxifloxacin, norfloxacin, ofloxacin, and rufloxacin), compared to those of trimethoprim-sulfamethoxazole (sxt) and ceftazidime on total biomass and cell viability of stenotrophomonas maltophilia biofilm. s. maltophilia attached rapidly to polystyrene, within 2 h of incubation, and then biofilm formation increased over time, reaching maximum growth at 24 h. in the presence of fluoroquinolo ... | 2004 | 14693533 |
| [post-marketing surveillance of antibacterial activities of cefozopran against various clinical isolates--ii. gram-negative bacteria]. | as a post-marketing surveillance, the in vitro antibacterial activities of cefozopran (czop), an agent of cephems, against various clinical isolates were yearly evaluated and compared with those of other cephems, oxacephems, carbapenems, monobactams, and penicillins. changes in czop susceptibility among bacteria were also evaluated with the bacterial resistance ratio calculated from the breakpoint mic. twenty-five species (4,154 strains) of gram-negative bacteria were isolated from the clinical ... | 2003 | 14692381 |
| hospital-acquired pneumonia: coverage and treatment adequacy of current guidelines. | the american thoracic society (ats) guideline for hospital-acquired pneumonia (hap) released in 1996 and the trouillet classification published in 1998 supply different rational foundations for the classification of patients with hap and for the selection of initial antibiotic therapy. the aims of this study were to assess the level of bacterial coverage and to assess and validate the adequacy of antibiotic strategy of each of these classifications. intensive care unit-admitted patients (n=71) w ... | 2003 | 14680072 |
| exposure to bioaerosols in a municipal sewage treatment plant. | microbiological air sampling was performed in a medium-size sewage treatment plant processing municipal wastewater from a city located in eastern poland. air samples for determination of the concentrations of viable mesophilic bacteria, gram-negative bacteria, thermophilic actinomycetes, fungi and endotoxin were collected at 12 sites associated with various phases of sewage treatment process. the concentrations of total mesophilic bacteria (both gram-positive and gram-negative) were within a ran ... | 2003 | 14677919 |
| identification of novel eubacteria from spent mushroom compost (smc) waste by dna sequence typing: ecological considerations of disposal on agricultural land. | a small study was undertaken to examine the microbiological characteristics of spent mushroom compost (smc), which is the major waste by-product of the mushroom industry and which is regularly disposed off by application to agricultural land. the primary aim of this study was to examine smc for the presence of faecal bacterial pathogens, including campylobacter spp., salmonella spp. and listeria monocytogenes. secondly it was desirable to quantify bacterial and fungal populations within smc, and ... | 2004 | 14672727 |
| [pathogenic bacteria in cystic fibrosis]. | since the cf gene identification in 1989 and despite the improvement of our knowledge in the physiopathology of the disease, bronchopulmonary infection determines the vital prognosis. following staphylococcus aureus infection, patients are colonized or colonized by pseudomonas aeruginosa, greatly involved in the pulmonary deterioration. other bacteria may be involved burkholderia cepacia, stenotrophomonas maltophilia, alcaligenes sp. intensive antibiotic treatment of primocolonisation helps to p ... | 2003 | 14671932 |
| bacteraemia in hospitalised patients with malignant blood disorders: a retrospective study of causative agents and their resistance profiles during a 14-year period without antibacterial prophylaxis. | bacteraemia is a major cause of morbidity and mortality in patients with haematological disorders during chemotherapy-induced neutropenia. the generally reported trend during the last two decades has been a gradual replacement of gram-negative bacilli by gram-positive cocci as the major causes of bacteraemia in neutropenic hosts. however, data that are unaffected by the use of antibacterial prophylaxis are scarce. our objective therefore was to study the incidence of bacteraemia with different m ... | 2003 | 14671614 |