Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| application of high-resolution melt curve analysis for classification of infectious bronchitis viruses in field specimens. | a real-time polymerase chain reaction (pcr)/high-resolution melt (hrm) curve analysis protocol was developed in our laboratory to differentiate infectious bronchitis (ib) virus reference strains. in the current study, this method was used to detect and classify ib viruses in field submissions. | 2010 | 20854299 |
| protective immune responses induced by in ovo immunization with recombinant adenoviruses expressing spike (s1) glycoprotein of infectious bronchitis virus fused/co-administered with granulocyte-macrophage colony stimulating factor. | infectious bronchitis virus (ibv) causes tremendous economic losses associated with production inefficiencies and mortality in poultry industry worldwide. in the present report, the recombinant adenoviruses expressing chicken granulocyte-macrophage colony stimulating factor (gm-csf) and s1 gene of nephropathogenic ibv were constructed and characterized. then, the immunological efficacy and protection against homologous ibv challenge were assessed in specific pathogen free (spf) chickens. the res ... | 2011 | 20850939 |
| molecular analysis of infectious bronchitis viruses isolated in slovenia between 1990 and 2005: a retrospective study. | fifteen infectious bronchitis viruses (ibv) isolated from broiler and broiler breeder flocks in slovenia between 1990 and 2005 were molecularly characterised. ibv strains were divided into four genotypes by the analysis of the s1 gene region. four strains belonged to the massachusetts genotype, one strain was placed into the qx genotype, one strain formed a cluster together with the b1648 strain and nine strains were classified into the 624/i genotype. nine slovenian strains of the 624/i genotyp ... | 2010 | 20844944 |
| a 15-year analysis of molecular epidemiology of avian infectious bronchitis coronavirus in china. | a comprehensive study of the epidemiology and pathogenicity of infectious bronchitis virus (ibv) in china was carried out by molecular characterization of the s1 gene from 46 isolates obtained for this study and 174 reference strains isolated over a 15-year period. nine types were found according to sequence analysis and phylogenetic study of the s1 gene. the co-circulation of multiple ibv types and the ongoing emergence of ibv variants are the epidemiological challenges in china. factors contri ... | 2011 | 20833270 |
| elucidation of the avian nucleolar proteome by quantitative proteomics using silac and changes in cells infected with the coronavirus infectious bronchitis virus. | the nucleolus is a dynamic subnuclear compartment involved in ribosome subunit biogenesis, regulation of cell stress and modulation of cellular growth and the cell cycle, among other functions. the nucleolus is composed of complex protein/protein and protein/rna interactions. it is a target of virus infection with many viral proteins being shown to localize to the nucleolus during infection. perturbations to the structure of the nucleolus and its proteome have been predicted to play a role in bo ... | 2010 | 20827733 |
| [avian infectious bronchitis disease in tunisia: seroprevalence, pathogenicity and compatibility studies of vaccine-field isolates]. | a sero-epidemiological study was carried out on 5660 sera collected, between 2006 and 2008, from different flocks in different regions of the country. the elisa results showed low levels of antibodies indicating vaccination failures. 45 to 69% of the flocks showed positive levels of antibodies and only 5 to 15% of these were protected. the pathogenicity studies of the tunisian field isolates tn20/00 and tn335/01 demonstrated high clinical and lesion scores indicating the pathogenic effect of the ... | 2009 | 20707223 |
| forsythoside a inhibits the avian infectious bronchitis virus in cell culture. | forsythoside a is a polyphenolic constituent of the fruits of forsythia suspensa vahl. which is widely used as an antiinflammatory agent in traditional chinese medicine. in the present study, the effects of forsythoside a on cell infection by avian infectious bronchitis virus were assessed. a real-time fluorescence quantitative pcr assay was used to determine mrna content of ibv n gene. the pretreatment of cells with forsythoside a, adding forsythoside a post infection of cells, and treatment of ... | 2011 | 20677175 |
| complete genome sequence and recombination analysis of infectious bronchitis virus attenuated vaccine strain h120. | the strain h120 of infectious bronchitis virus (ibv) is one of the earliest and representative attenuated live infectious bronchitis vaccine strains. to investigate the genomic feature of h120 and further understand its role in the epidemiology of ibv, complete genome of h120 was sequenced and compared with sequences of other ibv strains by phylogenetic and recombination analysis. the complete genome of h120 is 27631 nucleotides in length and has a similar structure with that of beaudette strain ... | 2010 | 20652731 |
| detection of infectious bronchitis virus strain n1/88 from the oviduct and feces of experimentally infected vaccinated and unvaccinated hens. | hens were vaccinated during the rearing phase with infectious bronchitis virus (ibv) vaccines commercially available in australia (vic s and a3) or left unvaccinated and then challenged with the n1/88 strain of ibv at 30 wk of age. oviduct and fecal samples were collected at regular intervals after n1/88 challenge. a locked nucleic acid probe-based reverse transcription real-time pcr test was designed and used to detect the ibv strain n1/88 from the oviduct and feces of unvaccinated and vaccinat ... | 2010 | 20634513 |
| treatment of eggshell abnormalities and reduced egg production caused by mycoplasma synoviae infection. | eggshell abnormalities were seen in the apex of eggs in two of three flocks of multi-age, hy-line layer chickens housed on a farm in northern italy. approximately 1.3% to 1.8% of eggs in one flock were affected, amounting to 300-400 eggs per day; the abnormalities resulted in a great deal of breakage and spoilage of healthy eggs. the mean weight of eggs was also reduced. egg abnormalities in a second flock were less severe. mycoplasma synoviae was detected in birds from both of the affected floc ... | 2010 | 20608549 |
| molecular epidemiology of avian infectious bronchitis in brazil from 2007 to 2008 in breeders, broilers, and layers. | multiple lineages of brazilian strains from 2007 to 2008 of avian infectious bronchitis virus (ibv) were detected in flocks of breeders, broilers, and layers. organs samples from 20 ibv-positive flocks with variable clinical signs were submitted to the partial amplification of s gene (nucleotides 726-1071) of ibv. fifteen of the 20 sequenced strains segregated in a unique brazilian cluster subdivided in three subclusters (brazil 01, 02, and 03). whereas three strains could be classified as massa ... | 2010 | 20608535 |
| host intraspatial selection of infectious bronchitis virus populations. | arkansas (ark)-type infectious bronchitis virus (ibv) subpopulations with an s gene sequence distinct from the vaccine predominant consensus were previously found in the upper respiratory tract of chickens within 3 days after inoculation. this finding indicated that a distinct virus subpopulation was rapidly positively selected by the chicken upper respiratory tract. we hypothesized that during host invasion, the replicating ibv population further changes as it confronts the distinct environment ... | 2010 | 20608523 |
| analysis of newcastle disease virus quasispecies and factors affecting the emergence of virulent virus. | genome sequence analysis of a number of avirulent field isolates of newcastle disease virus revealed the presence of viruses (within their quasispecies) that contained virulent f0 sequences. detection of these virulent sequences below the ~1% level, using standard cloning and sequence analysis, proved difficult, and thus a more sensitive reverse-transcription real-time pcr procedure was developed to detect both virulent and avirulent ndv f0 sequences. reverse-transcription real-time pcr analysis ... | 2010 | 20602243 |
| [expression and biological function analysis of chicken aminopeptidase n]. | to clone and express the gene encoding chicken aminopeptidase n (chapn), and analysis the biological function of chapn expressed in escherichia coli (e. coli). the chapn gene was amplified by rt-pcr from the kidney cells of chicken embryo and then cloned into the prokaryotic expression vector pcold-tf. recombinant expression plasmid of pcold-tf-chapn was constructed and then transformed into the competent e. coli bl21(de3) cells for expression under different conditions such as induction time an ... | 2010 | 20575434 |
| naturally occurring recombination between distant strains of infectious bronchitis virus. | new variants of infectious bronchitis virus (ibv) have emerged in australia despite its geographical isolation and intensive vaccination programs. in the present study, the 3' terminal 7.2 kb of the genome of a recently isolated variant of ibv (n1/03) was sequenced and compared with the sequences of classical and novel strains of ibv, the two main groups of these viruses in australia. the comparison revealed that recombination between classical and novel ibvs was responsible for the emergence of ... | 2010 | 20574643 |
| the cellular rna helicase ddx1 interacts with coronavirus nonstructural protein 14 and enhances viral replication. | the involvement of host proteins in the replication and transcription of viral rna is a poorly understood area for many rna viruses. for coronaviruses, it was long speculated that replication of the giant rna genome and transcription of multiple subgenomic mrna species by a unique discontinuous transcription mechanism may require host cofactors. to search for such cellular proteins, yeast two-hybrid screening was carried out by using the nonstructural protein 14 (nsp14) from the coronavirus infe ... | 2010 | 20573827 |
| comparative histopathology and immunohistochemistry of qx-like, massachusetts and 793/b serotypes of infectious bronchitis virus infection in chickens. | the aim of this study was to compare experimentally the pathogenicity and tissue distribution of the recently emerged qx-like strain of infectious bronchitis virus (ibv) with the widespread m41 and 793/b serotypes of the virus. histopathological and immunohistochemical methods were employed to define the main sites of virus replication. one-day-old specific pathogen free chickens were inoculated with five different qx-like strains, or with the m41 and 793/b ibv strains and monitored for 42 days ... | 2010 | 20570279 |
| genetic analysis revealed lx4 genotype strains of avian infectious bronchitis virus became predominant in recent years in sichuan area, china. | between 2008 and 2010, 19 strains of infectious bronchitis virus (ibv) were isolated from the vaccinated chicken flocks in sichuan province, china. the s1 genes of the isolates were amplified and sequenced. phylogenetic analysis revealed that the 19 isolates and 37 reference ibv strains can be grouped into eight genotypes. although ibvs of taiwan-i type, massachusetts type, and proventriculitis type were isolated, but most isolates were lx4 genotype. homology analysis of the sequences of s1 gene ... | 2010 | 20556502 |
| rapid heat-treatment attenuation of infectious bronchitis virus. | in the present study we describe the rapid development of an attenuated live vaccine for ga08, a new serotype of infectious bronchitis virus, using a heat-treatment method. incubation of the ga08 strain of ibv at 56 degrees c and passage in embryonated eggs was used as a method to fast track the attenuation process. the virus was incubated in a 56 degrees c water bath and aliquots were removed every 5 min for up to 1 h, and then each aliquot was inoculated into 10-day-old embryonated eggs. virus ... | 2010 | 20544430 |
| differential sensitivity of well-differentiated avian respiratory epithelial cells to infection by different strains of infectious bronchitis virus. | infectious bronchitis virus (ibv) is an avian coronavirus affecting the respiratory tract of chickens. to analyze ibv infection of the lower respiratory tract, we applied a technique that uses precision-cut lung slices (pclss). this method allows infection of bronchial cells within their natural tissue composition under in vitro conditions. we demonstrate that ibv strains 4/91, italy02, and qx infect ciliated and mucus-producing cells of the bronchial epithelium, whereas cells of the parabronchi ... | 2010 | 20538853 |
| proteomic analysis of purified coronavirus infectious bronchitis virus particles. | abstract: | 2010 | 20534109 |
| crystallization and preliminary x-ray diffraction studies of infectious bronchitis virus nonstructural protein 9. | avian infectious bronchitis virus (ibv), which causes respiratory disease in infected birds, belongs to coronavirus group 3. ibv encodes 15 nonstructural proteins (nsp2-nsp16) which play crucial roles in rna transcription and genome replication. nonstructural protein 9 (nsp9) has been identified as a protein that is essential to viral replication because of its single-stranded rna-binding ability. the gene segment encoding ibv nsp9 has been cloned and expressed in escherichia coli. the protein h ... | 2010 | 20516605 |
| quantitative proteomics using stable isotope labeling with amino acids in cell culture reveals changes in the cytoplasmic, nuclear, and nucleolar proteomes in vero cells infected with the coronavirus infectious bronchitis virus. | virus-host interactions involve complex interplay between viral and host factors, rendering them an ideal target for proteomic analysis. here we detail a high throughput quantitative proteomics analysis of vero cells infected with the coronavirus infectious bronchitis virus (ibv), a positive strand rna virus that replicates in the cytoplasm. stable isotope labeling with amino acids in cell culture (silac) was used in conjunction with lc-ms/ms to identify and quantify 1830 cellular and two viral ... | 2010 | 20467043 |
| a novel genotype of avian infectious bronchitis virus isolated in japan in 2009. | avian infectious bronchitis viruses (ibvs) isolated from commercial layer flocks kept in ibaraki prefecture in 2009 were genetically and serologically characterized. reverse transcription-pcr coupled with direct nucleotide sequencing and genbank blast database analysis of the hypervariable region of the s1 subunit of the virus spike gene showed that these isolates are genetically very different from the previously known ibv genotypes in japan. furthermore, none of the antisera used in this study ... | 2010 | 20460833 |
| immune response of aa broilers to ibv h120 vaccine and sodium new houttuyfonate. | in this report, 120 healthy one-day-old aa broilers were divided into six groups. groups 1-4 received 100, 200, 400 and 800 mg/l of sodium new houttuyfonate (snh) with ib vaccine h120 respectively. group 5 received pbs and h120 and group 6 il-2 and h120. the chickens were inoculated at 7 and 14 days of age. on 0, 7, 14, 21, 28 and 35 post first vaccination, the dynamic changes of peripheral lymphocyte proliferation, cytokine assays and serum antibody titers were assayed respectively by mtt metho ... | 2010 | 20451228 |
| functional and genetic studies of the substrate specificity of coronavirus infectious bronchitis virus 3c-like proteinase. | coronavirus (cov) 3c-like proteinase (3clpro), located in nonstructural protein 5 (nsp5), processes the replicase polyproteins 1a and 1ab (pp1a and pp1ab) at 11 specific sites to produce 12 mature nonstructural proteins (nsp5 to nsp16). structural and biochemical studies suggest that a conserved gln residue at the p1 position is absolutely required for efficient cleavage. here, we investigate the effects of amino acid substitution at the p1 position of 3clpro cleavage sites of infectious bronchi ... | 2010 | 20444893 |
| a multiplex reverse transcriptase-pcr assay for the genotyping of avian infectious bronchitis viruses. | infectious bronchitis viruses (ibvs) in taiwan have been divided into two genogroups, taiwan group i (tw-i) and taiwan group ii (tw-ii). heterologous mass-type strains are widely used as vaccines in the field. this work reports on a rapid and reliable multiplex reverse transcriptase-polymerase chain reaction (mrt-pcr) assay for the genotyping of ibvs. multiplex primer sets were designed to amplify the region covering hypervariable regions 1 and 2 of the s1 glycoprotein gene. several local strain ... | 2010 | 20408407 |
| viral respiratory diseases (ilt, ampv infections, ib): are they ever under control? | 1. the use of vaccines is the main approach to control of the economically important poultry viral respiratory diseases infectious laryngotracheitis (ilt), avian metapneumovirus (ampv) infections and infectious bronchitis (ib). this paper appraises the current methods of vaccine control in the light of the nature of each virus and epidemiological factors associated with each disease. 2. infectious laryngotracheitis virus (iltv) exists as a single type with a wide range of disease severity. it is ... | 2010 | 20390564 |
| induction of eggshell apex abnormalities in broiler breeder hens. | recently, the causal relationship between eggshell apex abnormalities (eaa) and mycoplasma synoviae was described. this eggshell pathology has only been documented in table egg layers both spontaneously and experimentally infected with m. synoviae, suggesting that meat-type layers are less prone to this condition. in this study the susceptibility of specified pathogen free (spf) broiler breeder hens to produce eggs with eaa after m. synoviae infection was assessed. five groups of 12 hens each we ... | 2010 | 20390548 |
| efficacy of infectious bronchitis virus vaccinations in the field: association between the alpha-ibv igm response, protection and vaccine application parameters. | infectious bronchitis virus (ibv) is, in spite of vaccination, still a major cause of respiratory problems in broilers and of poor egg production in breeders and layers in many parts of the world. a possible cause of the insufficient protection induced by vaccination is an inadequate application of the vaccine. this paper reports the results of two field studies. in the first, the results of the alpha-ibv igm enzyme-linked immunosorbent assay (elisa) on post-vaccination sera were compared with t ... | 2010 | 20390547 |
| a novel b-cell epitope of avian infectious bronchitis virus n protein. | this report describes the identification of a novel linear b-cell epitope at the n-terminus of the avian infectious bronchitis virus (ibv) nucleocapsid (n) protein. a monoclonal antibody designated 2d2 was prepared against the ibv n protein, and a series of 23 partially overlapping, tagged fragments of the ibv n gene were expressed. these peptides and five heterogeneous ibv strains were used to identify the epitope recognized by 2d2 using enzyme-linked immunosorbent assay (elisa) and western blo ... | 2010 | 20373999 |
| a protein chip designed to differentiate visually antibodies in chickens which were infected by four different viruses. | this study aimed to develop a visual protein chip that can differentiate the antibodies induced by avian influenza virus, newcastle disease virus, infectious bronchitis virus and infectious bursal disease virus, simultaneously. proteins from the four viruses were purified and spotted onto an aldehyde group-modified glass slide at 2mg/ml. after that, the protein chip was reacted with the corresponding positive serum against these viruses, hybridized with a colloidal gold-labeled secondary antibod ... | 2010 | 20362004 |
| development, standardization and assessment of pcr systems for purity testing of avian viral vaccines. | the european pharmacopoeia (ph. eur.) requires avian viral vaccines to be free of adventitious agents. purity testing is an essential quality requirement of immunological veterinary medicinal products (ivmps) and testing for extraneous agents includes monitoring for many different viruses. conventional virus detection methods include serology or virus culture, however, molecular tests have become a valid alternative testing method. nucleic acid testing (nat) is fast, highly sensitive and has a h ... | 2010 | 20338785 |
| evaluation of the sensitivity of pcr methods for the detection of extraneous agents and comparison with in vivo testing. | in this study the sensitivity of polymerase chain reaction (pcr) methods for the detection of newcastle disease virus (ndv), avian reovirus (arv), avian influenza virus (aiv) and avian infectious bronchitis virus (ibv) was compared to the sensitivity of the corresponding serological tests described in the european pharmacopoeia (ph. eur.). for this purpose, serial 10-fold dilutions of the respective inactivated vaccines were prepared and groups of spf chickens were vaccinated with a double dose ... | 2010 | 20338784 |
| understanding stress-induced immunosuppression: exploration of cytokine and chemokine gene profiles in chicken peripheral leukocytes. | at present, the poultry meat and egg industry has gained a lot of ground, being viewed as a provider of a healthy alternative to red meat and other protein sources. if this trend is to be maintained, solutions must be found to improve resistance of chickens to disease, which often is weakened by stressful conditions. in poultry, stress-induced immunosuppression is manifested by failures in vaccination and increased morbidity and mortality of flocks. currently, several modern cellular and molecul ... | 2010 | 20308420 |
| priming with a dna vaccine and boosting with an inactivated vaccine enhance the immune response against infectious bronchitis virus. | the methods of repeated immunization with inactivated vaccines have been used widely to increase antibody protection against infectious bronchitis virus (ibv). however, compared with dna vaccines, these methods usually induce poor cellular responses. in the present study, specific pathogen-free (spf) chickens were immunized intramuscularly with a dna vaccine carrying the main ibv structural genes (pvax1-s1, pvax1-m, and pvax1-n, respectively) and boosted with the ibv m41 strain inactivated vacci ... | 2010 | 20307574 |
| evolution of infectious bronchitis virus in taiwan: characterisation of rna recombination in the nucleocapsid gene. | avian infectious bronchitis virus (ibv) belongs to the coronaviridae family and causes significant economic loss in taiwan (tw), even in flocks that have been extensively immunised with massachusetts (mass)-serotype vaccines. phylogenetic analysis of all non-structural and most structural genes shows that tw ibv is genetically distinct from the us strain and more similar to chinese (ch) ibv. in contrast, the nucleocapsid (n) gene of tw ibv presents phylogenetic incongruence. rna recombination at ... | 2010 | 20299165 |
| immunogenicity and protective efficacy of a replication-defective infectious bronchitis virus vaccine using an adenovirus vector and administered in ovo. | in ovo vaccination remains an attractive option for a cost effective, uniform and mass application of vaccines for commercial poultry. however, the vaccines which can be delivered safely by this method are limited and there is no currently licensed embryo-safe vaccine against infectious bronchitis virus (ibv). in this study, a recombinant adenovirus expressing the s1 gene of nephropathogenic ibv (rad-s1) was constructed and the immune responses and protective efficacy against homologous challeng ... | 2010 | 20219540 |
| a simple and rapid strip test for detection of antibodies to avian infectious bronchitis virus. | a simple gold-immunochromatographic assay (gica) based on indirect reaction format was developed for rapid detection of antibodies against avian infectious bronchitis virus (ibv). the detection time of ibv igg by the gica was less than 10 min, whereas the hi test and the enzyme-linked immunosorbent assay (elisa) require 1-2 hr. reference sera against newcastle disease virus, infectious bursal disease, avian influenza virus h5 and h9 subtypes were all negatives for anti-ibv antibodies using the g ... | 2010 | 20215721 |
| genomic characterization of a proventriculitis-associated infectious bronchitis coronavirus. | transmissible proventriculitis associated with infectious bronchitis virus (ibv) was at first seen in eastern china in mid-1995, and is now endemic in china. herein, the complete genome sequence of a proventiculitis-associated infectious bronchitis coronavirus (zj971) was sequenced and analyzed. compared with the genome of the vaccine strain h120, zj971 had 54 nucleotide substitutions and a deletion in the 3'-utr. the substitutions were in the regions of nsp2-nsp5, nsp7, nsp12, nsp13, nsp15, s a ... | 2010 | 20195741 |
| swine intestine antimicrobial peptides inhibit infectious bronchitis virus infectivity in chick embryos. | infectious bronchitis virus (ibv), the causative agent of infectious bronchitis, results in respiratory disease, nephritis, and poor egg production and quality in chicken. antimicrobial peptides possess potent antimicrobial activities and are regarded as promising therapeutic alternatives in the fight against microorganisms. to assess the in vitro antiviral activity of swine intestine antimicrobial peptides (siamp) against ibv, 45 chick embryos were randomly assigned into 3 groups, 15 for each g ... | 2010 | 20181861 |
| identification of intertypic recombinant infectious bronchitis viruses from slaughtered chickens. | avian coronavirus infectious bronchitis virus (ibv) poses a major threat to the global poultry industry. new ibv geno- and serotypes are continually reported. however, information on ibv prevalence is not frequently addressed in these reports. this study reports on a viral surveillance program in taiwan from 2005 to 2006 with sampling conducted in poultry slaughterhouses. the genetic features of the obtained field isolates were investigated using sequence analysis and simplot analysis. a 1-direc ... | 2010 | 20181858 |
| characterization of a novel live attenuated infectious bronchitis virus vaccine candidate derived from a korean nephropathogenic strain. | a nephropathogenic k2/01 strain of infectious bronchitis virus (ibv) was attenuated by 170 serial passages in embryonated chicken eggs for possible use as a future ibv vaccine strain. high-growth properties and narrow tissue tropisms (limited replication in respiratory tracts) were achieved by the adaptation process. unlike the parent strain, the attenuated strain (k2p170) was safe in day-old specific-pathogen-free chicks since replication of the virus did not induce mortality and nephritis, and ... | 2010 | 20153350 |
| avian coronavirus infectious bronchitis virus susceptibility to botanical oleoresins and essential oils in vitro and in vivo. | anti-coronaviral activity of a mixture of oleoresins and essential oils from botanicals, designated qr448(a), was examined in vitro and in vivo. treatment of avian infectious bronchitis virus (ibv) with qr448(a) reduced the virus titer as measured in two laboratory host systems, vero e6 cells and embryonating eggs. the effect of qr448(a) on ibv in chickens was also investigated. administering qr448(a) to chickens at a 1:20 dilution by spray, 2h before challenge with ibv was determined to be the ... | 2010 | 20096315 |
| characteristics of a very virulent infectious bursal disease virus from california. | an outbreak of infectious bursal disease (ibd) in two california layer flocks resulted in the isolation of two infectious bursal disease viruses designated ra and rb. increased mortality plus gross and histopathology in the layer flocks suggested ra and rb could be very virulent infectious bursal disease virus (vvibdv). preliminary studies indicated that high mortality resulted when bursa homogenates from the layer farms were used to inoculate specific-pathogen-free (spf) chicks. in addition, ra ... | 2009 | 20095162 |
| classification of ibv s1 genotypes by direct reverse transcriptase-polymerase chain reaction (rt-pcr) and relationship between serotypes and genotypes of strains isolated between 1998 and 2008 in japan. | in this study, we attempted to establish a simple detection method for classification of ibv s1 genotypes by direct reverse transcriptase-polymerase chain reaction (rt-pcr). then, to evaluate the usefulness of the s1 genotype-specific rt-pcr, we examined the relationship between s1 genotypes and serotypes of ibv in japan. sequencing of the s1 genes of ibv and phylogenetic tree analysis were conducted. on the basis of the sequencing data of the s1 genotype samples, we determined primer sets speci ... | 2010 | 20093802 |
| multivalent dna vaccine enhanced protection efficacy against infectious bronchitis virus in chickens. | for efficacious dna vaccine development against infectious bronchitis virus (ibv), the immunogenicity of a multivalent dna vaccine was evaluated. three expression plasmids each targeting spike protein (s1), nucleocapsid protein (n), and membrane protein (m) of ibv were prepared. chickens were immunized with either individual plasmids (monovalent) or with a combination of all plasmids (multivalent). immunization with the multivalent dna vaccine induced synergistic augmentation of humoral and cell ... | 2009 | 20046025 |
| isolation and genetic analysis revealed no predominant new strains of avian infectious bronchitis virus circulating in south china during 2004-2008. | twenty-seven strains of avian infectious bronchitis virus (ibv) were isolated from dead or diseased chickens at different chicken farms in south china during 2004-2008, of which the s1 gene was sequenced. phylogenetic analysis of the s1 gene sequences of the isolated 27 strains together with 29 strains published in genbank revealed that all ibv strains except for one isolated and one published were clustered into six distinct genotypes i-vi. 26 isolated strains belong to genotypes i, ii, and iii ... | 2010 | 20022714 |
| emergence of a group 3 coronavirus through recombination. | analyses of turkey coronavirus (tcov), an enteric disease virus that is highly similar to infectious bronchitis virus (ibv) an upper-respiratory tract disease virus in chickens, were conducted to determine the adaptive potential, and genetic changes associated with emergence of this group 3 coronavirus. strains of tcov that were pathogenic in poults and nonpathogenic in chickens did not adapt to cause disease in chickens. comparative genomics revealed two recombination sites that replaced the sp ... | 2010 | 20022075 |
| comparison of the pathogenicity of qx-like, m41 and 793/b infectious bronchitis strains from different pathological conditions. | five qx-like infectious bronchitis virus (ibv) strains, isolated from different field outbreaks and two reference ibv strains of known serotypes (m41 and 793/b), were used in the present study to investigate and compare their pathogenicity for 1-day-old specific pathogen free chickens. the ability of the strains to inhibit trachea epithelium ciliary activity, to induce immune response, to replicate and to cause histopathological lesions in designated organs was followed by repeated samplings dur ... | 2009 | 19937534 |
| effect of a live mycoplasma synoviae vaccine on the production of eggshell apex abnormalities induced by a m. synoviae infection preceded by an infection with infectious bronchitis virus d1466. | an experimental study was conducted to assess the effect of a live mycoplasma synoviae vaccine (vaxsafe ms; bioproperties pty ltd, ringwood, victoria, australia) on m. synoviae-induced eggshell apex abnormalities (eaa). four experimental groups of specified-pathogen-free white laying hens were made. all groups were inoculated with infectious bronchitis virus d1466 at 18 weeks of age. one group did not receive further treatment (non-vaccinated non-challenged (nvnc)). two groups were vaccinated at ... | 2009 | 19937520 |
| pathogenicity and antigenicity of a new variant of korean nephropathogenic infectious bronchitis virus. | despite the existence of an active vaccination program, recently emerged strains of nephropathogenic infectious bronchitis virus (ibv) in korea have caused significant economic losses in the poultry industry. in this study, we assessed the pathogenic and antigenic characteristics of a k-iib type field strain of ibv that emerged in korea since 2003, such as kr/q43/06. specific pathogen free 1-week-old chickens exhibited severe respiratory symptoms (dyspnea) and nephropathogenic lesions (swollen k ... | 2009 | 19934604 |
| multiplex polymerase chain reaction for the detection and differentiation of avian influenza viruses and other poultry respiratory pathogens. | a multiplex reverse transcription-pcr (mrt-pcr) was developed and standardized for the detection of type a influenza viruses, avian influenza virus (aiv) subtype h7, h9, and h5 hemagglutinin gene with simultaneous detection of 3 other poultry respiratory pathogens, newcastle disease virus (ndv), infectious bronchitis virus (ibv), and infectious laryngotracheitis virus (iltv). seven sets of specific oligonucleotide primers were used in this study for the m gene of aiv and hemagglutinin gene of su ... | 2009 | 19903950 |
| rapid detection of infectious bursal disease virus by reverse transcription loop-mediated isothermal amplification assay. | a reverse transcription loop-mediated isothermal amplification (rt-lamp) assay was developed for the rapid identification of infectious bursal disease virus (ibdv). the rt-lamp assay used a set of 4 primers to amplify the viral protein 2 gene of ibdv for the detection of ibdv, showing not only high efficiency but also analytic specificity. the data demonstrated that the rt-lamp assay detected 30 different ibdv isolates, had no cross-reaction with 3 other avian viruses (infectious bronchitis viru ... | 2009 | 19901286 |
| identification of a novel linear b-cell epitope in the m protein of avian infectious bronchitis coronaviruses. | this report describes the identification of a novel linear b-cell epitope at the c-terminus of the membrane (m) protein of avian infectious bronchitis virus (ibv). a monoclonal antibody (mab) (designated as 15e2) against the ibv m protein was prepared and a series of 14 partially-overlapping fragments of the ibv m gene were expressed with a gst tag. these peptides were subjected to enzyme-linked immunosorbent assay (elisa) and western blotting analysis using mab 15e2 to identify the epitope. a l ... | 2009 | 19851732 |
| characterization of new variants of avian infectious bronchitis virus in tunisia. | three infectious bronchitis virus (ibv) strains, isolated from suspected tunisian broiler flocks, were characterized as variant viruses using genotyping and serotyping techniques. they were compared with commonly used vaccine strains, including 793/b, d274, and massachusetts types. reverse transcription-pcr-restriction fragment length polymorphism, nucleotide sequencing, and genbank blast database analyses of the hypervariable region of the s1 subunit of the virus spike gene showed that the thre ... | 2009 | 19848084 |
| detection and molecular characterization of gene 3 and 5 of turkey coronavirus from turkeys with severe enteritis in brazil. | turkey coronavirus (tcov) is a causative agent associated with poult enteritis and mortality syndrome (pems) in turkeys worldwide. the disease is an acute, highly contagious enteric disease that is characterized by depression, anorexia, diarrhea, and high mortality in commercial turkey flocks. the presence of tcov in 12 intestinal-content samples, from turkey flocks aged between 10 and 104 days and exhibiting severe enteritis, was monitored during the period of 2004 to 2006. tcov detection was a ... | 2009 | 19848072 |
| reverse transcription loop-mediated isothermal amplification for the rapid detection of infectious bronchitis virus in infected chicken tissues. | a reverse transcription loop-mediated isothermal amplification (rt-lamp) assay targeting the nucleocapsid phosphoprotein gene of infectious bronchitis virus (ibv) was developed. the detection limits for the ibv rt-lamp assay were 10(1) 50% egg infection dose (eid(50)) per 50 microl of titrated viruses and no cross-reaction of ibv rt-lamp was found when tested with other viruses including newcastle disease virus (ndv), avian reovirus (arv), and infectious laryngotrachietis virus (iltv) due to the ... | 2010 | 19835950 |
| the replicase gene of avian coronavirus infectious bronchitis virus is a determinant of pathogenicity. | we have previously demonstrated that the replacement of the s gene from an avirulent strain (beaudette) of infectious bronchitis virus (ibv) with an s gene from a virulent strain (m41) resulted in a recombinant virus (beaur-m41(s)) with the in vitro cell tropism of the virulent virus but that was still avirulent. in order to investigate whether any of the other structural or accessory genes played a role in pathogenicity we have now replaced these from the beaudette strain with those from m41. t ... | 2009 | 19816578 |
| vaccine efficacy against ontario isolates of infectious bronchitis virus. | infectious bronchitis (ib) is an economically important viral disease with worldwide distribution. every country with an intensive poultry industry has infectious bronchitis virus (ibv). the virus rapidly spreads from bird to bird through horizontal transmission by aerosol or ingestion. sentinel bird studies were carried out in southern ontario and ibv has been isolated from layer flocks. genetic analysis of the s1 region of the strains showed that they were not vaccine related. the pathogenicit ... | 2009 | 19794894 |
| the infection of primary avian tracheal epithelial cells with infectious bronchitis virus. | here we introduce a culture system for the isolation, passaging and amplification of avian tracheal epithelial (ate) cells. the ate medium, which contains chicken embryo extract and fetal bovine serum, supports the growth of ciliated cells, goblet cells and basal cells from chicken tracheas on fibronectin- or matrigel-coated dishes. non-epithelial cells make up less than 10% of the total population. we further show that ate cells support the replication and spread of infectious bronchitis virus ... | 2010 | 19793537 |
| protection of chickens against infectious bronchitis by a recombinant fowlpox virus co-expressing ibv-s1 and chicken ifngamma. | a fowlpox virus expressing the chicken infectious bronchitis virus (ibv) s1 gene of the lx4 strain (rfpv-ibvs1) and a fowlpox virus co-expressing the s1 gene and the chicken type ii interferon gene (rfpv-ibvs1-chifngamma) were constructed. these viruses were assessed for their immunological efficacy on specific-pathogen-free (spf) chickens challenged with a virulent ibv. although the antibody levels in the rfpv-ibvs1-chifngamma-vaccinated group were lower than those in the attenuated live ib vac ... | 2009 | 19786144 |
| development of a real-time taqman rt-pcr assay for the detection of infectious bronchitis virus in chickens, and comparison of rt-pcr and virus isolation. | a sensitive and specific method for the diagnosis of infectious bronchitis virus (ibv) is of great importance. in this study the development of a real-time taqman rt-pcr targeting the highly conserved nucleocapsid (n) gene of ibv and including an internal pcr control is described. the assay was specific for ibv and did not detect other avian pathogens, including turkey coronaviruses. a comparative limit of detection was determined for m41, an embryo-adapted strain, and is/885/00, a poorly embryo ... | 2010 | 19781572 |
| inhibition of protein kinase r activation and upregulation of gadd34 expression play a synergistic role in facilitating coronavirus replication by maintaining de novo protein synthesis in virus-infected cells. | a diversity of strategies is evolved by rna viruses to manipulate the host translation machinery in order to create an optimal environment for viral replication and progeny production. one of the common viral targets is the alpha subunit of eukaryotic initiation factor 2 (eif-2alpha). in this report, we show that phosphorylation of eif-2alpha was severely suppressed in human and animal cells infected with the coronavirus infectious bronchitis virus (ibv). to understand whether this suppression i ... | 2009 | 19776135 |
| detection and differentiation of four poultry diseases using asymmetric reverse transcription polymerase chain reaction in combination with oligonucleotide microarrays. | asymmetric reverse transcription polymerase chain reaction (rt-pcr) and microarrays were combined to distinguish 4 viruses, including avian influenza virus (aiv), newcastle disease virus (ndv), infectious bronchitis virus (ibv), and infectious bursal disease virus (ibdv), and hemagglutinin (ha) subtypes h5, h7, and h9, and neuraminidase (na) subtypes n1 and n2 of aiv. the aiv matrix protein (m), and ha and na genes, ibv nucleoprotein (np) gene, ndv np gene, and ibdv a fragment gene were cloned i ... | 2009 | 19737757 |
| comparative evaluation of real-time pcr and conventional rt-pcr during a 2 year surveillance for influenza and respiratory syncytial virus among children with acute respiratory infections in kolkata, india, reveals a distinct seasonality of infection. | acute respiratory tract infections (artis) are one of the most common causes of morbidity and mortality in young children worldwide. influenza virus and respiratory syncytial virus (rsv) are the predominant aetiological agents during seasonal epidemics, and thus rapid and sensitive molecular tests for screening for such agents and timely identification of epidemics are required. this study compared real-time quantitative pcr (qpcr) with conventional rt-pcr for parallel identification of influenz ... | 2009 | 19713363 |
| detection and molecular characterization of infectious bronchitis virus isolated from recent outbreaks in broiler flocks in thailand. | thirteen field isolates of infectious bronchitis virus (ibv) were isolated from broiler flocks in thailand between january and june 2008. the 878-bp of the s1 gene covering a hypervariable region was amplified and sequenced. phylogenetic analysis based on that region revealed that these viruses were separated into two groups (i and ii). ibv isolates in group i were not related to other ibv strains published in the genbank database. group 1 nucleotide sequence identities were less than 85% and am ... | 2009 | 19687622 |
| serological studies of infectious bronchitis vaccines against japanese field isolates of homologous and heterologous genotypes. | the genetic diversity of the partial s1 gene involving the hyper variable region for infectious bronchitis (ib) vaccine strains in japan were compared with those of ib virus isolated from the field in japan. field isolates have mainly been classified into three major genotypes, jp-i, jp-ii and jp-iii, since 2003; however, the 4/91 genotype was detected from recent field isolates in japan. the virus neutralization (vn) activity with vaccine immunized serum was investigated to evaluate the protect ... | 2009 | 19652475 |
| expression, crystallization and preliminary x-ray diffraction analysis of the n-terminal domain of nsp2 from avian infectious bronchitis virus. | avian infectious bronchitis virus (ibv) is a prototype of the group iii coronaviruses and encodes 15 nonstructural proteins which make up the transcription/replication machinery. the nsp2 protein from ibv has a unique and novel sequence and has no experimentally confirmed function in replication, whereas it has been proposed to be crucial for early viral infection and may inhibit the early host immune response. the gene that encodes a double-mutant ibv nsp2 n-terminal domain (residues 9-393 of t ... | 2009 | 19652340 |
| cpg oligodeoxynucleotides activate innate immune response that suppresses infectious bronchitis virus replication in chicken embryos. | the understanding of innate immune modulation by pathogens and immune-modulating agents, including synthetic oligodeoxynucleotides (cpg odns), has offered several new approaches to improve prophylactic and therapeutic strategies against infectious diseases in humans and animals. however, in this regard not much work has been done in avian medicine. in the present study, we analyzed the kinetics of interferon (ifn), cytokine, and chemokine mrna expression in chicken embryonic spleen at 6 hr, 24 h ... | 2009 | 19630234 |
| infectious bronchitis virus field vaccination coverage and persistence of arkansas-type viruses in commercial broilers. | to determine the coverage of infectious bronchitis virus (ibv) vaccine field boost in commercial broilers, estimate the relative amount of vaccine virus in the trachea, and follow the clearance of the vaccine, we collected approximately 100 tracheal swabs at various times postvaccination from 10 different flocks and used real-time reverse transcriptase-pcr (rt-pcr) to detect the virus. this allowed us to detect vaccine virus in as few as 3% of the birds in a flock of 20,000 birds with a 95% conf ... | 2009 | 19630221 |
| characterization of a new genotype and serotype of infectious bronchitis virus in western africa. | between 2002 and 2007, more than 1000 chickens from commercial farms, live bird markets and backyard farms in nigeria and niger were tested for the presence of the infectious bronchitis virus (ibv) genome. phylogenetic analysis of full-length sequences of the spike 1 (s1) gene revealed a new genotype of ibv that we refer to as 'ibadan'. the minimum genetic distance to the closest 'non-ibadan' strains (uk/7/93 at the nucleotide level; h120 and m41 at the amino acid level) reached 24 and 32 % at t ... | 2009 | 19625463 |
| genetically diverse coronaviruses in wild bird populations of northern england. | infectious bronchitis virus (ibv) causes a costly respiratory viral disease of chickens. the role of wild birds in the epidemiology of ibv is poorly understood. we detected diverse coronaviruses by pcr in wildfowl and wading birds in england. sequence analysis showed some viruses to be related to ibv. | 2009 | 19624927 |
| molecular characterization and pathogenicity of infectious bronchitis coronaviruses: complicated evolution and epidemiology in china caused by cocirculation of multiple types of infectious bronchitis coronaviruses. | to monitor and study the molecular epidemiology, evolution and pathogenicity of infectious bronchitis viruses (ibvs) in china in recent years and further our knowledge of the evolution of ibvs. | 2009 | 19590226 |
| the protective immune response against infectious bronchitis virus induced by multi-epitope based peptide vaccines. | peptide vaccine was found to be an effective and powerful approach to a variety of pathogens. to explore multi-epitope based peptide vaccines against infectious bronchitis virus (ibv), the immunogenic peptides were fused to the 3' terminal of glutathione s transferase gene (gst) and expressed in escherichia coli. elisa and western blot analysis showed that the purified fusion proteins had excellent immune activity with chicken anti-ibv serum. during the vaccination course, the candidate peptide ... | 2009 | 19584555 |
| proteolytic activation of the spike protein at a novel rrrr/s motif is implicated in furin-dependent entry, syncytium formation, and infectivity of coronavirus infectious bronchitis virus in cultured cells. | the spike (s) protein of the coronavirus (cov) infectious bronchitis virus (ibv) is cleaved into s1 and s2 subunits at the furin consensus motif rrfrr(537)/s in virus-infected cells. in this study, we observe that the s2 subunit of the ibv beaudette strain is additionally cleaved at the second furin site (rrrr(690)/s) in cells expressing s constructs and in virus-infected cells. detailed time course experiments showed that a peptide furin inhibitor, decanoyl-arg-val-lys-arg-chloromethylketone, b ... | 2009 | 19553314 |
| coadministration of chicken gm-csf with a dna vaccine expressing infectious bronchitis virus (ibv) s1 glycoprotein enhances the specific immune response and protects against ibv infection. | various approaches have been developed to improve the efficacy of dna vaccination, such as the use of plasmids expressing cytokines as molecular adjuvants. the purpose of the present study was to determine whether co-administration of a plasmid containing a chicken granulocyte-macrophage colony-stimulating factor (gm-csf) gene and a plasmid containing the s1 gene of infectious bronchitis virus (ibv) could enhance the immune response and protection efficacy in chickens against challenge by virule ... | 2009 | 19543689 |
| altered pathogenicity, immunogenicity, tissue tropism and 3'-7kb region sequence of an avian infectious bronchitis coronavirus strain after serial passage in embryos. | in this study, we attenuated a chinese lx4-type nephropathogenic infectious bronchitis virus (ibv) strain, ck/ch/lhlj/04v, by serial passage in embryonated chicken eggs. based on sequence analysis of the 3'-7kb region, the ck/ch/lhlj/04v virus population contained subpopulations with a mixture of genetic mutants. the titers of the virus increased gradually during serial passage, but the replication capacity decreased in chickens. the virus was partially attenuated at passage 40 (p40) and p70, an ... | 2009 | 19523910 |
| genetic analysis of the s1 gene of 4/91 type infectious bronchitis virus isolated in japan. | s1 gene sequences for infectious bronchitis virus (ibv) strains of the 4/91 genotype (commonly called 793b) isolated from field outbreaks in japan were analyzed to ascertain the relationship to 4/91 vaccine strain. three field isolates (jp/wakayama/2003, jp/iwate/2005 and jp/saitama/2006) from flocks not immunized with a 4/91 type live ibv vaccine and one isolate (jp/wakayama-2/2004) from a flock immunized with a 4/91 type live vaccine were examined. the amino acid identities among jp/wakayama/2 ... | 2009 | 19498283 |
| comparative analysis of the effect of glycyrrhizin diammonium and lithium chloride on infectious bronchitis virus infection in vitro. | the effects of glycyrrhizin diammonium (gd) and lithium chloride (licl) on cell infection by avian infectious bronchitis virus (ibv) were investigated using cytopathic effect observation, plaque-reduction assay and reverse transcriptase-polymerase chain reaction. the anti-viral effect of gd and licl on virus, on virus-infected cells or on cells pre-treated by both drugs was analysed, respectively. our results showed that gd had a direct antiviral activity, leading to complete inhibition of cell ... | 2009 | 19468938 |
| massachusetts live vaccination protects against a novel infectious bronchitis virus s1 genotype dmv/5642/06. | four infectious bronchitis virus (ibv) isolates were recovered from commercial broiler chicken flocks located on the delmarva peninsula (east coast of the united states) in the spring of 2006. sequence analysis of the s1 subunit of the spike glycoprotein gene showed the four isolates were highly related to each other (> or = 99.6% nucleotide identity; > or = 98.9% amino acid identity). basic local alignment search tool analysis indicated the highest s1 amino acid identity of isolate dmv/5642/06, ... | 2009 | 19432014 |
| rapid subtyping of h9n2 influenza virus by a triple reverse transcription polymerase chain reaction. | the aim of this study was to develop a rapid, cost-saving triple reverse transcription polymerase chain reaction (triple rt-pcr) for subtyping h9n2 avian influenza viruses (aivs). the three primer pairs for amplification of target sequences of nucleoprotein (np), hemagglutinin (ha) and neuraminidase (na) genes, respectively, were designed for subtyping the viruses in the triple rt-pcr. the sensitivity of triple rt-pcr was found to be 10(2) copies per reaction for each of np, h9 and n2 gene. the ... | 2009 | 19428570 |
| identification of in vivo-interacting domains of the murine coronavirus nucleocapsid protein. | the coronavirus nucleocapsid protein (n), together with the large, positive-strand rna viral genome, forms a helically symmetric nucleocapsid. this ribonucleoprotein structure becomes packaged into virions through association with the carboxy-terminal endodomain of the membrane protein (m), which is the principal constituent of the virion envelope. previous work with the prototype coronavirus mouse hepatitis virus (mhv) has shown that a major determinant of the n-m interaction maps to the carbox ... | 2009 | 19420077 |
| towards construction of viral vectors based on avian coronavirus infectious bronchitis virus for gene delivery and vaccine development. | manipulation of the coronavirus genome to accommodate and express foreign genes is an attractive approach for gene delivery and vaccine development. by using an infectious cloning system developed recently for the avian coronavirus infectious bronchitis virus (ibv), the enhanced green fluorescent protein (egfp) gene, the firefly luciferase gene and several host and viral genes (eif3f, sars orf6, dengue virus 1 core protein gene) were inserted into various positions of the ibv genome, and the eff ... | 2009 | 19409420 |
| broadly targeted multiprobe qpcr for detection of coronaviruses: coronavirus is common among mallard ducks (anas platyrhynchos). | coronaviruses (covs) can cause trivial or fatal disease in humans and in animals. detection methods for a wide range of covs are needed, to understand viral evolution, host range, transmission and maintenance in reservoirs. a new concept, "multiprobe qpcr", which uses a balanced mixture of competing discrete non- or moderately degenerated nuclease degradable (taqman) probes was employed. it provides a broadly targeted and rational single tube real-time reverse transcription pcr ("nqpcr") for the ... | 2009 | 19406168 |
| genetic diversity of avian infectious bronchitis viruses in japan based on analysis of s2 glycoprotein gene. | to understand the genetic diversity of the s2 gene of infectious bronchitis viruses (ibv) isolated in japan, we determined the nucleotide sequences of these ibvs using the reverse transcriptase polymerase chain reaction method coupled with direct sequencing. ibv isolated in japan were classified into six different groups by phylogenetic analysis based on the s2 gene. however, the classification based on the s2 gene of ibv isolated in japan was different for some of the strains from those obtaine ... | 2009 | 19346695 |
| [use of multiplex pcr method with real-time detection for differential diagnosis of respiratory viral infections]. | multiplex real-time polymerase chain reaction test-system with fluorescent detection (rt-pcr) for simultaneous identification of main agents of acute respiratory viral infections: influenza a (iav) and b viruses (ibv), parainfluenza viruses types 1, 2, 3, 4 (piv 1 - 4), adenoviruses (adv), respiratory syncitial virus (rsv), rhinoviruses (rv) and enteroviruses (ev), in presence internal positive control (ipc) represented by vaccine strain of rubella virus ra 27/3. using multiplex rt-pcr method, r ... | 2009 | 19338239 |
| infectious bronchitis virus in jordanian chickens: seroprevalence and detection. | infectious bronchitis (ib) is one of the most important viral diseases of poultry; it causes major economic losses to the poultry industry. this study was conducted to investigate the prevalence of infectious bronchitis virus (ibv) in commercial chicken flocks in jordan. serum samples from 70 commercial chicken flocks (40 broilers, 18 layers, and 12 broiler breeders) free from respiratory disease were collected and screened for the presence of massachusetts-41 (m-41), d274, and 4/91 strain antig ... | 2009 | 19337618 |
| molecular characterization of avian infectious bronchitis virus strains from outbreaks in argentina (2001-2008). | twenty infectious bronchitis virus isolates were recovered from broilers and layers in different outbreaks amongst commercial poultry flocks in different geographic regions of argentina from 2001 to 2008. the viruses were isolated from the tracheas, lungs, and caecal tonsils of birds that were showing respiratory signs. further analysis based on their nucleotide and amino acid sequences in hypervariable region (hvr) 1 and the intervening sequence including hvrs 1 and 2 (hvr1/2) of the s1 gene wa ... | 2009 | 19322714 |
| rapid detection and non-subjective characterisation of infectious bronchitis virus isolates using high-resolution melt curve analysis and a mathematical model. | infectious bronchitis virus (ibv) is a coronavirus that causes upper respiratory, renal and/or reproductive diseases with high morbidity in poultry. classification of ibv is important for implementation of vaccination strategies to control the disease in commercial poultry. currently, the lengthy process of sequence analysis of the ibv s1 gene is considered the gold standard for ibv strain identification, with a high nucleotide identity (e.g. > or =95%) indicating related strains. however, this ... | 2009 | 19301093 |
| interaction of the coronavirus infectious bronchitis virus membrane protein with beta-actin and its implication in virion assembly and budding. | coronavirus m protein is an essential component of virion and plays pivotal roles in virion assembly, budding and maturation. the m protein is integrated into the viral envelope with three transmembrane domains flanked by a short amino-terminal ectodomain and a large carboxy-terminal endodomain. in this study, we showed co-purification of the m protein from coronavirus infectious bronchitis virus (ibv) with actin. to understand the cellular factors that may be involved in virion assembly, buddin ... | 2009 | 19287488 |
| sequence and phylogenetic analysis of s1, s2, m, and n genes of infectious bronchitis virus isolates from malaysia. | two malaysian infectious bronchitis virus isolates, mh5365/95 and v9/04 were characterized based on sequence and phylogenetic analyses of s1, s2, m, and n genes. nucleotide sequence alignments revealed many point mutations, short deletions, and insertions in s1 region of both ibv isolates. phylogenetic analysis of s1 gene and sequences analysis of m gene indicated that mh5365/95 and v9/04 belong to non-massachusetts strain. however, both isolates share only 77% identity. analysis based on s1 gen ... | 2009 | 19242786 |
| identification of sequence changes responsible for the attenuation of avian infectious bronchitis virus strain arkansas dpi. | infectious bronchitis virus (ibv) is the causal agent of infectious bronchitis, which still remains one of the most important poultry diseases worldwide because of numerous serotypes and variants. a virulent strain of ibv, isolated from arkansas (ark), was propagated in embryonated eggs (ark dpi 11). following 101 serial passages in embryonated eggs, an attenuated strain of ibv was established (ark dpi 101) that does not induce histopathological lesions in the tracheae of infected chicks. to ide ... | 2009 | 19219402 |
| immunohistochemistry for detection of avian infectious bronchitis virus strain m41 in the proventriculus and nervous system of experimentally infected chicken embryos. | infectious bronchitis virus primarily induces a disease of the respiratory system, different ibv strains may show variable tissue tropisms and also affect the oviduct and the kidneys. proventriculitis was also associated with some new ibv strains. aim of this study was to investigate by immunohistochemistry (ihc) the tissue tropism of avian infectious bronchitis virus (ibv) strain m41 in experimentally infected chicken embryos. | 2009 | 19196466 |
| innovation and discovery: the application of nucleic acid-based technology to avian virus detection and characterization. | polymerase chain reaction (pcr)-based approaches to the detection, differentiation and characterization of avian pathogens continue to be developed and refined. the pcrs, or reverse transcriptase-pcrs, may be general, designed to detect all or most variants of a pathogen, or to be serotype, genotype or pathotype specific. progress is being made with respect to making nucleic acid approaches more suitable for use in diagnostic laboratories. robotic workstations are now available for extraction of ... | 2001 | 19184952 |
| sequence analysis of nephropathogenic infectious bronchitis virus strains of the massachusetts genotype in beijing. | the first 440 nucleotides of the s1 part of the spike protein (s) gene of three nephropathogenic infection bronchitis virus (ibv) strains (bj1, bj2 and bj3), isolated from three rural chicken farms of beijing with outbreaks of avian infectious bronchitis, were sequenced after reverse transcriptasenested polymerase chain reaction amplification. the sequence data were analyzed and compared with the standard ibv strains m41, beaudette, vaccine strain h120 and chinese vaccine strain d41. the three i ... | 2001 | 19184943 |
| safety and efficacy of water-in-oil-in-water emulsion vaccines containing newcastle disease virus haemagglutinin-neuraminidase glycoprotein. | subunit vaccines containing haemagglutinin-neuraminidase (hn) glycoprotein of newcastle disease virus (ndv), formulated as water-in-oil-in-water (w/o/w) emulsions, were prepared. first, the suitable constituents of a w/o/w emulsion adjuvant were investigated with polyvalent vaccines using ndv, infectious bronchitis virus and haemophilus paragallinarum. the w/o/w emulsion adjuvant, composed of the antigen in phosphate-buffered saline (pbs), liquid paraffin, squalene, diglyceryl monooleate, polyso ... | 2001 | 19184940 |
| protection of chickens against renal damage caused by a nephropathogenic infectious bronchitis virus. | the ability of the infectious bronchitis (ib) ma5 and 4/91 live-attenuated vaccines to protect against kidney damage caused by a nephropathogenic strain of ib virus (b1648) was investigated. protection parameters considered were gross and microscopic renal pathology, and the use of a polymerase chain reaction to detect ib rna in kidney tissue. by each parameter, ma5 vaccine alone provided poor protection, but 4/91 alone or the combined program both protected well. | 2001 | 19184927 |
| epidemiology of infectious bronchitis virus in belgian broilers: a retrospective study, 1986 to 1995. | infectious bronchitis virus (ibv) was isolated from each of 236 broiler flocks that had respiratory infection (86%), impaired growth, enteritis and/or nephritis (14%), over a 10-year period from 1986 to 1995 in belgium. among them, 65% of the investigated flocks had not been vaccinated against infectious bronchitis. type-specific reverse transcriptase polymerase chain reactions (rt-pcrs) were used after propagation of the isolates in embryonated eggs in order to detect and differentiate massachu ... | 2001 | 19184926 |
| detection of a coronavirus from turkey poults in europe genetically related to infectious bronchitis virus of chickens. | intestinal contents of 13-day-old turkey poults in great britain were analysed as the birds showed stunting, unevenness and lameness, with 4% mortality. at post mortem examination, the main gross features were fluid caecal and intestinal contents. histological examination of tissues was largely unremarkable, apart from some sections that showed crypt dilation and flattened epithelia. negative contrast electron microscopy of caecal contents revealed virus particles, which in size and morphology h ... | 2001 | 19184921 |