Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| a replicon trans-packaging system reveals the requirement of nonstructural proteins for the assembly of bovine viral diarrhea virus (bvdv) virion. | a selective trans-packaging system was developed to produce and isolate bovine viral diarrhea virus (bvdv) pseudo-particles with complementing reporter replicons and their packaging proteins expressed in trans with recombinant vaccinia virus. the encapsidation of replicon rns3-5b was dependent not only on the in trans expression of structural proteins c, e(rns), e1 and e2, but also the nonstructural proteins, p7 and contiguous precursor ns2-3-4a. nonstructural p7, ns4b, ns5a or ns5b could be exp ... | 2009 | 19327808 |
| identification of a lipid kinase as a host factor involved in hepatitis c virus rna replication. | a functional screen of an adenovirus-delivered shrna library that targets approximately 4500 host genes was performed to identify cellular factors that regulate hepatitis c virus (hcv) sub-genomic rna replication. seventy-three hits were further examined by sirna oligonucleotide-directed knockdown, and silencing of the pi4ka gene was demonstrated to have a significant effect on the replication of a hcv genotype 1b replicon. using transient sirna oligonucleotide transfections and stable shrna kno ... | 2009 | 19304308 |
| inhibition of sphingosine kinase by bovine viral diarrhea virus ns3 is crucial for efficient viral replication and cytopathogenesis. | sphingosine 1-phosphate (s1p) is a bioactive sphingolipid implicated in diverse cellular functions including survival, proliferation, tumorigenesis, inflammation, and immunity. sphingosine kinase (sphk) contributes to these functions by converting sphingosine to s1p. we report here that the nonstructural protein ns3 from bovine viral diarrhea virus (bvdv), a close relative of hepatitis c virus (hcv), binds to and inhibits the catalytic activity of sphk1 independently of its serine protease activ ... | 2009 | 19293152 |
| effects of on-arrival versus delayed clostridial or modified live respiratory vaccinations on health, performance, bovine viral diarrhea virus type i titers, and stress and immune measures of newly received beef calves. | stress, commonly associated with weaning, marketing, and shipment of feeder cattle, can compromise immune function, and vaccine administration during immunosuppression may reduce vaccine efficacy and calf growth. four treatments were compared in a 2 x 2 factorial arrangement to evaluate the effect of on-arrival (d 0) vs. delayed (d 14) administration of clostridial (clos) and respiratory (resp) vaccines on health, performance, bovine viral diarrhea virus (bvdv) antibody titers, and physiological ... | 2009 | 19286815 |
| comparative moleculo-immunological analysis of swamp- and riverine-type water buffaloes responses. | this moleculo-epidemiological and immunological study through cytokine response assessment was done to know the dynamics of cytokines in the initiation, persistence and association to physiological changes of a particular pathogen in water buffaloes. this is important to understand the magnitude and behavior of disease progression. water buffalo blood samples gathered from different places in the philippines revealed a 9.4%, 27.6%, 10.3% and 4.4% prevalence of bovine viral diarrhea virus (bvdv), ... | 2009 | 19285880 |
| seroprevalence and characterization of pestivirus infections in small ruminants and new world camelids in switzerland. | the seroprevalence of pestivirus infections in small ruminants and new world camelids in switzerland was determined. in 5'059 sera of sheep from 382 herds, 503 sera of goats from 54 herds and 109 sera of alpacas and lamas from 53 herds, population prevalences of 16.1% (sheep), 25.4% (goats) and 4.6% (new world camelids), respectively, were found. in order to determine the source of infection, the serological reactions were further characterized by cross-neutralization against two pestiviruses re ... | 2009 | 19263380 |
| bovine viral diarrhea virus (bvdv) associated with single in vivo-derived and in vitro-produced preimplantation bovine embryos following artificial exposure. | the objective was to determine the average amount of bovine viral diarrhea virus (bvdv) associated with single in vivo-derived and in vitro-produced bovine embryos following recommended processing procedures for embryos. in vivo-derived and in vitro-produced bovine embryos at 7d post-fertilization were exposed (for 2h) to 2 x 10(5-7) cell culture infective dose (ccid(50))/ml of sd-1 (a noncytopathic, type 1a strain of bvdv), and then washed according to international embryo transfer society (iet ... | 2009 | 19246081 |
| effect of the bovine viral diarrhoea virus (bvdv) infection on dairy calf rearing. | the aim of this study was to compare the cumulative incidence of mortality, clinical diarrhoea and respiratory disease in calves, during their first six months of age, in herds with different bovine viral diarrhoea virus (bvdv) infection status. calves' health indicators were tested by comparing proportions in 101 farms with dissimilar infection condition. the results indicate that there was a significant relationship between the bvdv status (actively infected herd or not) and the cumulative inc ... | 2009 | 19230944 |
| novel imino sugar derivatives demonstrate potent antiviral activity against flaviviruses. | imino sugars, such as n-butyl-deoxynojirimycin and n-nonyl-deoxynojirimycin (nndnj), are glucose analogues that selectively inhibit cellular alpha-glucosidase i and ii in the endoplasmic reticulum and exhibit antiviral activities against many types of enveloped viruses. although these molecules have broad-spectrum antiviral activity, their development has been limited by a lack of efficacy and/or selectivity. we have previously reported that a dnj derivative with a hydroxylated cyclohexyl side c ... | 2009 | 19223639 |
| prevalence of bovine viral diarrhea virus infections in alpacas in the united states. | to determine the prevalence of bovine viral diarrhea virus (bvdv)-infected alpaca herds in the united states and investigate factors associated with seropositive herd status and, subsequently, determine the proportion of animals within seropositive alpaca herds that are persistently infected (pi) carriers for bvdv, obtain information regarding previous herd exposure to bvdv, determine titers of anti-bvdv antibodies of dams, and ascertain whether individual seropositive crias had received supplem ... | 2009 | 19222364 |
| phylogeny, classification and evolutionary insights into pestiviruses. | the genus pestivirus comprises four established species: bovine viral diarrhoea viruses 1 (bvdv-1) and 2 (bvdv-2), border disease virus (bdv), and classical swine fever virus (csfv); and a tentative species, pestivirus of giraffe. additional pestiviruses have been identified and suggested for recognition as novel subgroups/species. to achieve a reliable phylogeny as the basis for classification of pestiviruses, a molecular dataset of 56 pestiviruses and 2089 characters, comprising the 5'utr, com ... | 2009 | 19167739 |
| control of bovine virus diarrhoea at the herd level: reducing the risk of false negatives in the detection of persistently infected cattle. | the need to detect and eliminate cattle persistently infected (pi) with bovine viral diarrhoea virus (bvdv) is key to the control of bvd and has been shown to be very effective in eradicating bvdv from infected herds. however, because of pitfalls in the detection procedures, some pi animals can be missed and, as a result, are not identified and removal is delayed. the high prevalence of bvdv in cattle populations in some countries (such as belgium and neighbouring countries) means there is a hig ... | 2010 | 19157928 |
| prevalence of bovine viral diarrhoea virus (bvdv) antibodies among sheep and goats in india. | the aim of this study was to determine the prevalence of pestivirus antibodies in sheep and goats in india. a total of 2803 serum samples collected between 2004 and 2008 from 1777 sheep in 92 flocks and 1026 goats in 63 flocks belonging to 13 states were tested by competition elisa for detection of pestivirus antibodies. in sheep, the true prevalence rate was 23.4% (95% confidence interval: 22.9%-27.0%) and in goats it was 16.9% (95% ci: 16.4%-21.3%). the flock level seroprevalence was 66.3% for ... | 2009 | 19153817 |
| safety and efficacy of vaccination of seronegative bulls with modified-live, cytopathic bovine viral diarrhea viruses. | the objectives were to vaccinate peri-pubertal bulls with a modified-live vaccine consisting of cytopathic bvdv strains singer and 296 and evaluate the resulting: (a) transient shed of modified-live, cytopathic bvdv in semen; (b) risk of prolonged testicular infection; and (c) protection against subsequent testicular infection due to viral challenge. seronegative, peri-pubertal bulls were vaccinated subcutaneously with a standard dose of vaccine (n=11) or were maintained as unvaccinated controls ... | 2009 | 19144395 |
| disseminated bovine viral diarrhea virus in a persistently infected alpaca (vicugna pacos) cria. | bovine viral diarrhea virus (bvdv) is an emerging infectious pathogen of concern to the alpaca industry. a 4-month-old, intact, male alpaca cria was diagnosed as persistently infected with bvdv on the basis of repeated positive antemortem polymerase chain reaction (pcr) and virus isolation (vi) assays and negative serologic titers to bvdv. immunohistochemistry, real-time reverse transcription pcr, and vi performed on tissues collected at necropsy demonstrated disseminated bvdv-1b infection. viru ... | 2009 | 19139518 |
| detection of bovine viral diarrhea virus from three water buffalo fetuses (bubalus bubalis) in southern italy. | bovine viral diarrhea virus (bvdv) is an important pathogen that primarily infects ruminants, leading to several clinical problems including abortion. bvdv-specific antibodies were reported in a wide range of hosts within domestic and wildlife animal populations, and serological studies also indicated bvdv infection in buffaloes. the purpose of this study was to analyze the presence of bvdv in 2 water buffalo (bubalus bubalis) herds with a history of abortion. virus isolation from aborted fetuse ... | 2009 | 19139516 |
| seroprevalence and risk factors for the presence of ruminant pestiviruses in the dutch swine population. | swine can be infected with classical swine fever virus (csfv), as well as ruminant pestiviruses: bovine viral diarrhoea virus (bvdv), and border disease virus (bdv). cross-reactions between pestiviruses occur, both regarding protective immunity and in diagnostic tests. the presence of bvdv and bdv in a swine population may thus affect the transmission of csfv, but also the diagnosis of a csfv infection. in this study, the seroprevalence against bvdv and bdv in two categories of swine, sows and f ... | 2009 | 19128896 |
| genetic characterization of bovine viral diarrhea viruses isolated from persistently infected calves born to dams vaccinated against bovine viral diarrhea virus before breeding. | to collect and partially characterize strains of bovine viral diarrhea viruses(bvdvs) isolated from persistently infected (pi) calves born to vaccinated dams, determine genetic diversity of the isolated viruses, and identify regional distribution of genetically similar virus subpopulations. | 2009 | 19119952 |
| evaluation of economic effects and the health and performance of the general cattle population after exposure to cattle persistently infected with bovine viral diarrhea virus in a starter feedlot. | to evaluate economic effects and health and performance of the general cattle population after exposure to cattle persistently infected (pi) with bovine viral diarrhea virus (bvdv) in a feedlot. | 2009 | 19119951 |
| a sensitive and efficient detection method for bovine viral diarrhea virus (bvdv) in single preimplantation bovine embryos. | the objective was to develop a method to accurately and efficiently detect minute amounts of bovine viral diarrhea virus (bvdv) associated with a single embryo. there are two major challenges for bvdv detection in a single embryo: the test sensitivity and the efficiency of viral molecule recovery. these become even more critical when attempts are made to detect bvdv infections that occurred naturally, not through artificial exposure of the embryos to high affinity bvdv strains. we have developed ... | 2009 | 19118890 |
| distribution of bovine viral diarrhea virus antigen in aborted fetal and neonatal goats by immunohistochemistry. | bovine viral diarrhea virus (bvdv) infection in goats can result in severe reproductive losses, with abortion rates reaching 80%. infection with bvdv in aborted goat fetuses and stillborn kids can result in placentitis, encephalitis, myocarditis, and thymic depletion. this study investigates the distribution of viral antigen within the organ systems of aborted goat fetuses, stillborn kids, and nonviable kids infected with bvdv at various stages of gestation using immunohistochemistry (ihc). viru ... | 2009 | 19112115 |
| bovine viral diarrhea virus abortion in goats housed with persistently infected cattle. | twenty-four border disease virus-seronegative, pregnant, mixed breed goats were experimentally comingled with 3 heifers persistently infected with bovine viral diarrhea virus type 2a (bvdv-2a). twelve of the 24 exposed does aborted. twenty-nine fetuses and 16 placentas from affected does were submitted to the oklahoma animal disease diagnostic laboratory for a necropsy examination. infection with bvdv was confirmed with a combination of immunohistochemistry, bvdv-2 polymerase chain reaction, and ... | 2009 | 19112114 |
| antibody response against three widespread bovine viruses is not impaired in holstein cattle carrying bovine leukocyte antigen drb3.2 alleles associated with bovine leukemia virus resistance. | due to the wide dissemination of bovine leukemia virus (blv) infection among dairy cattle, control and eradication programs based on serological detection of infected cattle and subsequent culling face a major economic task. in argentina, genetic selection of cattle carrying alleles of the bovine leukocyte antigen (bola) drb3.2 gene associated with blv-infection resistance, like *0902, emerges as the best additional tool toward controlling virus spread. a potential risk in expanding or segregati ... | 2009 | 19109295 |
| study on continuous (254 nm) and pulsed uv (266 and 355 nm) lights on bvd virus inactivation and its effects on biological properties of fetal bovine serum. | both continuous uv lights and pulsed uv lasers have potentials to inactivate known and emerging viruses. bovine viral diarrhea virus (bvdv), from the pestivirus genus, is known to be a common viral contamination in (fetal) bovine serum (fbs). also, bvdv has been used in the blood product industry as a surrogate for hepatitis c virus (hcv), due to its similarity in structure and genome. germicidal uv lamp with the wavelength of 254 nm and nd:yag laser (pulsed uv laser) in its third and fourth har ... | 2009 | 19095459 |
| regeneration and characterization of a recombinant bovine viral diarrhea virus and determination of its efficacy to cross the bovine placenta. | the capacity of different bovine viral diarrhea virus (bvdv) strains to cause transplacental infection is variable. bvdv strain sd-1 was isolated from a persistently infected heifer. its genome represents the only reported nucleotide sequence of a noncytopathic viral isolate determined without cell culture passage in the laboratory. thus, sd-1 might possess biological advantages over other ncp bvdv strains to be used as a model virus for investigation of viral transplacental transmission. to eva ... | 2009 | 19067148 |
| [new furano- and pyrrolo[2,3-d]pyrimidine nucleosides and their 5'-triphosphates: synthesis and biological properties]. | bicyclic furano[2,3-d]pyrimidine ribonucleosides were synthesized by pd(0)- and cui-catalyzed coupling of 5-iodouridine with terminal alkynes. the treatment of the resulting nucleosides with ammonia or methylamine solution in aqueous alcohol resulted in pyrrolo- and n(7)-methylpyrrolo[2,3-d]pyrimidine nucleosides. 5'-o-triphosphates of bicyclic nucleosides were obtained by the treatment of the nucleosides with pocl3 in the presence of a "proton sponge." the 5'-o-triphosphates are not substrates ... | 2008 | 19060941 |
| genotyping and phylogenetic analysis of bovine viral diarrhea virus isolates from bvdv infected alpacas in north america. | over a three-year period, 2004-2007, greater than 12,000 alpacas in the united states were screened by real-time rt-pcr to identify alpacas persistently infected (pi) with bovine viral diarrhea virus (bvdv). a total of 46 bvd viruses were isolated from pi alpacas or diagnostic samples from alpacas. forty-three us alpaca bvdv isolates and 3 canadian isolates were analyzed by comparison of nucleotide sequences of two viral genomic regions, the 5'-utr and the n(pro) gene to determine their genetic ... | 2009 | 19059738 |
| reproductive tract disease associated with inoculation of pregnant white-tailed deer with bovine viral diarrhea virus. | to inoculate white-tailed deer (odocoileus virginianus) during the sixth or seventh week of gestation with bovine viral diarrhea virus (bvdv) and observe for signs of reproductive tract disease during a 182-day period. | 2008 | 19046011 |
| the viral rnase e(rns) prevents ifn type-i triggering by pestiviral single- and double-stranded rnas. | interferon (ifn) type-i is of utmost importance in the innate antiviral defence of eukaryotic cells. the cells express intra- and extracellular receptors that monitor their surroundings for the presence of viral genomes. bovine viral diarrhoea virus (bvdv), a pestivirus of the family flaviviridae, is able to prevent ifn synthesis induced by poly(ic), a synthetic dsrna. the evasion of innate immunity might be a decisive ability of bvdv to establish persistent infection in its host. we report that ... | 2009 | 19041350 |
| testing thermal resistance of viruses. | representative viral strains recommended for virucidal testing of biocides in human medicine were used for testing viral resistance to dry heat using the new keredusy hot instrument. the results demonstrate that poliovirus type 1 could be inactivated by treatment at 75 degrees c for 1 h. for inactivation of adenovirus type 5, 2 h at 85 degrees c was needed. the infectivity of polyomavirus sv40 could only be influenced significantly by a temperature of 95 degrees c over a period of 1 h, whereas v ... | 2009 | 19039515 |
| antiviral activity of benzimidazole derivatives. i. antiviral activity of 1-substituted-2-[(benzotriazol-1/2-yl)methyl]benzimidazoles. | forty-three 2-[(benzotriazol-1/2-yl)methyl]benzimidazoles, bearing either linear (dialkylamino)alkyl- or bulkier (quinolizidin-1-yl)alkyl moieties at position 1, were evaluated in cell-based assays for cytotoxicity and antiviral activity against viruses representative of two of the three genera of the flaviviridae family, i.e. flaviviruses (yellow fever virus (yfv)) and pestiviruses (bovine viral diarrhoea virus (bvdv)), as hepaciviruses can hardly be used in routine cell-based assays. compounds ... | 2008 | 19035566 |
| inactivation of virus in intravenous immunoglobulin g using solvent/detergent treatment and pasteurization. | the safety of plasma derived medicinal products, such as immunoglobulin, depends on viral inactivation steps that are incorporated into the production process. several attempts have been made to validate the effectiveness of these inactivation methods against a range of physio-chemically diverse viruses. treatment with solvent/detergent (s/d) and pasteurization (p) has been continuously used in our igg production and these methods were analysed in this study as models of viral inactivation. bovi ... | 2008 | 19029665 |
| coinfection is an important factor in epidemiological studies: the first serosurvey of the aoudad (ammotragus lervia). | despite being considered an invasive ungulate outside its native range (north africa), little information exists regarding the role of the aoudad (also called barbary sheep, ammotragus lervia) as a pathogen reservoir. furthermore, in most epidemiological surveys the potential role of coinfections (e.g. a first infection may make the host more immuno-competent or susceptible against a second pathogen) as a risk factor is often neglected. in this study we first performed a serological survey for s ... | 2009 | 19020912 |
| genetic diversity of pestivirus isolates in cattle from western austria. | the genetic diversity of bovine viral diarrhoea virus (bvdv) isolates in infected cattle from tyrol and vorarlberg (austria) was investigated. blood samples were collected within the compulsory austrian bvdv control programme during 2005 and 2006. the 5'-untranslated region (5'-utr) and partially the n-terminal autoprotease (n(pro)) were amplified by one-step reverse transcriptase-polymerase chain reaction (rt-pcr) and the pcr products were subsequently sequenced. phylogenetic analysis based on ... | 2009 | 19019571 |
| persistent fetal infection with bovine viral diarrhea virus differentially affects maternal blood cell signal transduction pathways. | the consequences of viral infection during pregnancy include impact on fetal and maternal immune responses and on fetal development. transplacental infection in cattle with noncytopathic bovine viral diarrhea virus (ncpbvdv) during early gestation results in persistently infected (pi) fetuses with life-long viremia and susceptibility to infections. infection of the fetus during the third trimester or after birth leads to a transient infection cleared by a competent immune system. we hypothesized ... | 2009 | 19018046 |
| backscattering particle immunoassays in wire-guide droplet manipulations. | abstract: a simpler way for manipulating droplets on a flat surface was demonstrated, eliminating the complications in the existing methods of open-surface digital microfluidics. programmed and motorized movements of 10 mul droplets were demonstrated using stepper motors and microcontrollers, including merging, complicated movement along the programmed path, and rapid mixing. latex immunoagglutination assays for mouse immunoglobulin g, bovine viral diarrhea virus and escherichia coli were demons ... | 2008 | 19014703 |
| differential expression of the type i interferon pathway during persistent and transient bovine viral diarrhea virus infection. | persistent infection with bovine viral diarrhea virus (bvdv) serves as a reservoir for the perpetuation of infection in cattle populations and causes a range of adverse effects on the health of the host. to study the interactions of the virus with the host, gene expression was compared in the blood of persistently infected (pi) and uninfected steer, and in the blood and tissues of pi fetuses, transiently infected (ti), and uninfected bovine fetuses. microarray analysis of pi steer blood revealed ... | 2009 | 19014339 |
| collective experiences of adventitious viruses of animal-derived raw materials and what can be done about them. | contamination of animal-derived raw materials with viruses, mycoplasmas, bacteria and fungi is common. these contaminants can interfere with the diagnosis of viral infection, and vaccines produced using infected cell cultures could lead to seroconversion or disease in the vaccinated animal. the purity, safety and efficacy of viral vaccines requires testing of the ingredients, cell substrates and final product. methods for detection of viruses, especially bovine viral diarrhea virus, in nutrient ... | 1998 | 19003405 |
| genetic analysis of a bovine viral diarrhea virus 2 isolate from slovakia. | the identification and genetic characterization of bovine viral diarrhea virus (bvdv) isolate 17237 detected in western slovakia is described. the analysis of 5'-untranslated region (5'-utr), autoprotease (npro) gene, and structural genes (c, erns, e1, e2) was carried out. the percentage of nucleotide and deduced amino acid identity in analyzed genes implied that the isolate was closely related to the bovine viral diarrhea virus 2 (bvdv-2). furthermore, the phylogenetic analysis revealed that th ... | 2008 | 18999890 |
| identification of a bovine mucosal-disease virus isolated in sweden as myxovirus para-influenzae 3. | 1960 | 18990778 | |
| genetic diversity of bovine viral diarrhea virus 1: italian isolates clustered in at least seven subgenotypes. | bovine viral diarrhea virus (bvdv) is an economically important pathogen of cattle. two approved species are recognized, namely bvdv-1 and bvdv-2. to date, only 4 subgenotypes of bvdv-2 are known, and at least 11 distinct subgenotypes have been detected for bvdv-1. in a previous study, the genetic characteristics of 38 field isolates of bvdv from northern italy were investigated, and all 38 isolates were classified as bvdv-1 and could be assigned to 5 different subgenotypes, namely bvdv-1b, bvdv ... | 2008 | 18987229 |
| evidence for persistent bovine viral diarrhea virus infection in a captive mountain goat (oreamnos americanus). | bovine viral diarrhea (bvd) viruses are pestiviruses that have been isolated from domestic and wild ruminants. there is serologic evidence of pestiviral infection in more than 40 species of free-range and captive mammals. vertical transmission can produce persistently infected animals that are immunotolerant to the infecting strain of bovine viral diarrhea virus (bvdv) and shed virus throughout their lives. seven species (white-tailed deer, mouse deer, eland, domestic cattle, alpaca, sheep, and ... | 2008 | 18987224 |
| prevalence of infectious agents in free-ranging white-tailed deer in northeastern mexico. | the objectives of this study were to determine the prevalence of antibodies against brucellosis, leptospirosis, infectious bovine rhinotracheitis virus, and bovine viral diarrhea virus (bvdv) in white-tailed deer (odocoileus virginianus) in northeastern mexico. deer (n=521) were captured from helicopter using a netgun on 15 ranches covering 62,114 ha in the states of coahuila, nuevo leon, and tamaulipas during spring 2004. the prevalence of antibodies against leptospira, infectious bovine rhinot ... | 2008 | 18957659 |
| evaluation of automated nucleic acid extraction methods for virus detection in a multicenter comparative trial. | five european veterinary laboratories participated in an exercise to compare the performance of nucleic acid extraction robots. identical sets of coded samples were prepared using serial dilutions of bovine viral diarrhoea virus (bvdv) from serum and cell culture propagated material. each laboratory extracted nucleic acid from this panel using available robotic equipment (12 separate instruments, comprising 8 different models), after which the processed samples were frozen and sent to a single l ... | 2009 | 18952126 |
| bovine viral diarrhea virus is inactivated when whole milk from persistently infected cows is heated to prepare semen extender. | bovine viral diarrhea virus (bvdv) can be present in cryopreserved bovine semen and be transmitted through artificial insemination. because bvdv can be shed in milk, the virus might also be introduced as a contaminant of milk-based semen extenders. thus, the purpose of this study was to evaluate the epidemiologic risk of using heated, bvdv-contaminated milk to prepare semen extender. milk was obtained from cows free of and persistently infected (pi) with bvdv. six replicates of milk samples were ... | 2009 | 18950967 |
| monitoring of bvdv in a vaccinated herd by testing milk for antibodies to ns3 protein. | 2008 | 18931356 | |
| bovine viral diarrhea virus infection affects the expression of proteins related to professional antigen presentation in bovine monocytes. | the complete annotation of the cattle genome allows reliable protein identification by tandem mass spectrometry (ms(2)) and greatly facilitates proteomics. previously, we reported that differential detergent fractionation (ddf) analysis of bovine monocytes reveals proteins related to antigen pattern recognition, uptake and presentation to immunocompetent lymphocytes. here we have identified 47 bovine proteins, involved in immune function of professional antigen-presenting cells (apc) that have b ... | 2009 | 18930168 |
| prevalence of bovine viral diarrhoea virus antibodies among the industrial dairy cattle herds in suburb of mashhad-iran. | mashhad is a major dairy production in iran. the subject of this study was to survey the seroprevalence of bovine viral diarrhea virus (bvdv) infection using an indirect enzyme-linked immunosorbent assay (elisa) test in industrial dairy cattle herds in suburb of mashhad-iran. totally, 141 serum samples were tested. none of the herds had been vaccinated against bvdv. commercial indirect elisa kit was used. the herds divided to 3 sizes as cow population. they were included: small, medium and large ... | 2009 | 18839325 |
| a stochastic model to assess the risk of introduction of bovine viral diarrhea virus to beef cow-calf herds. | a spreadsheet model using monte carlo simulation was designed to evaluate the introduction of bovine viral diarrhea virus (bvdv) to cow-calf farms and the effect of different testing strategies. risks were modeled to include imports to the cow-calf herd and stocker calves imported to adjacent pastures. the number of persistently infected (pi) animals imported and the probability of bvdv introduction were monitored for three herd sizes, four import profiles, and six testing strategies. importing ... | 2009 | 18838184 |
| cohabitation of pregnant white-tailed deer and cattle persistently infected with bovine viral diarrhea virus results in persistently infected fawns. | economic losses due to infection with bovine viral diarrhea virus (bvdv) have prompted introduction of organized control programs. these programs primarily focus on the removal of persistently infected (pi) animals, the main source of bvdv transmission. recently, persistent bvdv infection was demonstrated experimentally in white-tailed deer, the most abundant wild ruminant in north america. contact of cattle and white-tailed deer may result in interspecific bvdv transmission and birth of persist ... | 2009 | 18834678 |
| transmission of bovine viral diarrhea virus (bvdv) via in vitro-fertilized embryos to recipients, but not to their offspring. | the objective was to assess the potential of day-7, ivp zona pellucida-intact blastocysts to transmit bovine viral diarrhea virus (bvdv) to embryo recipients. embryos were exposed (1h) to two non-cytopathic (ncp) biotypes, either ny-1 (type 1) or two concentrations of pa-131 (type 2), washed 10 times, and transferred into recipients (two embryos/recipient) free of bvdv and its antibody. six (30.0%) of the 20 pregnancies were lost after 30 d following transfer of the embryos exposed to the type 1 ... | 2009 | 18834622 |
| beta-defensin antibiotic peptides in the innate immunity of the buffalo: in vivo and in vitro studies. | beta-defensin antimicrobial peptides are multifunctional biomolecules, which are a major component of the oxygen-independent microbicidal system of buffalo polymorphonuclear (pmn) cells. they have great potential for use as proteomic biomarkers of host cell responses in the presence of microbial agents. on purifying these peptides by rp-hplc, four defensin peptides were revealed. the results from testing against escherichia coli, staphylococcus aureus, streptococcus pyogenes, candida albicans, r ... | 2008 | 18826332 |
| bovine viral diarrhea virus is a suitable viral vector for stable expression of heterologous gene when inserted in between n(pro) and c genes. | bovine viral diarrhea virus (bvdv) is a group of small enveloped viruses with a single-stranded, positive-oriented rna genome of approximately 12.3 kb. bvdv genome directs the production of a viral polyprotein that is subsequently cleaved to release the mature viral proteins. to explore the potential of using bvdv as viral vector for stable expression of heterologous genes, egfp2a was inserted in between n(pro) and c genes of a noncytopathic type-i bvdv strain sd1. egfp2a was designed with egfp ... | 2008 | 18824056 |
| bovine viral diarrhoea virus infection alters global transcription profiles in bovine endothelial cells. | bovine viral diarrhoea viruses (bvdv) are significant pathogens of cattle worldwide. these viruses exist in both non-cytopathic and cytopathic biotypes. non-cytopathic bvdv can establish persistent lifelong infections in cattle and are a frequent contaminant of biological reagents such as cell cultures and foetal bovine serum. we identified commercially available bovine aortic endothelial cells (baecs) contaminated with bvdv. in this study, to determine if bvdv alters endothelial gene transcript ... | 2008 | 18817290 |
| double immunization strategy with a bohv-4-vectorialized secreted chimeric peptide bvdv-e2/bohv-1-gd. | a bovine herpesvirus 4 was isolated from the milk cell fraction of a healthy cow and his full genome cloned as a bacterial artificial chromosome. so cloned viral genome was used as a vector platform to deliver in vitro and in vivo an optimized secreted chimeric peptide obtained by the fusion of the bovine viral diarrhoea virus glycoprotein e2 ectodomain with the bovine herpesvirus 1 glycoprotein d ectodomain. recombinant virus infected cells robustly expressed and secreted the chimeric peptide i ... | 2008 | 18812200 |
| rnase-dependent inhibition of extracellular, but not intracellular, dsrna-induced interferon synthesis by erns of pestiviruses. | recombinant pestivirus envelope glycoprotein e(rns) has been shown to interfere with dsrna-induced interferon (ifn-alpha/beta) synthesis. this study demonstrated that authentic, enzymically active e(rns) produced in mammalian cells prevented a dsrna-induced ifn response when present in the supernatant of bovine cells. strikingly, ifn synthesis of cells expressing e(rns) was eliminated after extracellular addition, but not transfection, of dsrna. importantly, the same applied to cells infected wi ... | 2008 | 18796719 |
| persistent bovine viral diarrhea virus infection in wild cervids of colorado. | bovine viral diarrhea virus (bvdv) is a significant viral pathogen of domestic cattle. worldwide, there is evidence of bvdv exposure and infection in wild ungulates; however, the frequency and significance of such events are unknown. to determine the prevalence and distribution of colorado deer, elk, and moose persistently infected (pi) with bvdv, a cross-sectional study was conducted using full-thickness ear tissue samples collected from animals presented to the colorado division of wildlife fo ... | 2008 | 18776103 |
| serological evaluation of precolostral serum samples to detect bovine viral diarrhea virus infections in large commercial dairy herds. | the authors propose that screening newborn calves for bovine viral diarrhea virus (bvdv) antibody prior to colostrum feeding is a useful strategy to detect herds with endemic bvdv infection. in the current study, precolostral serum samples of newborn calves in 2 minnesota and 2 california dairy farms were examined. precolostral bvdv antibodies were detected by serum neutralization and enzyme-linked immunosorbent assay in 7.4% (33/446) and 6.2% (32/515) of newborn calves in the california and min ... | 2008 | 18776097 |
| using singular perturbations to reduce an epidemiological model: application to bovine viral diarrhoea virus within-herd spread. | studying the spread of a pathogen in a managed metapopulation such as cattle herds in a geographical region often requires to take into account both the within- and between-herd transmission dynamics. this can lead to high-dimensional metapopulation systems resulting from the coupling of several within-herd transmission models. to tackle this problem, we aim in this paper at reducing the dimension of a tractable but realistic dynamical system reproducing the within-herd spread. the context chose ... | 2009 | 18775439 |
| impact of persistent bovine viral diarrhea viral infection on the duration and level of shedding of escherichia coli o157 in calves. | the goal of this study was to determine whether calves persistently infected with bovine viral diarrhea virus (bvdv) and inoculated with escherichia coli o157 will shed the organism at a higher concentration and for a longer duration than calves not infected with bvdv. | 2008 | 18767975 |
| bovine herpesvirus type 1 (bhv-1) and bovine viral diarrhoea virus (bvdv) infections in dairy herds: self clearance and the detection of seroconversions against a new atypical pestivirus. | the epidemiology of bovine herpesvirus type 1 (bhv-1) and bovine viral diarrhoea virus (bvdv) was studied in a population of small dairy herds that had not been vaccinated. bulk tank milk samples of 186 herds in thailand were collected four times between 2002 and 2004. serum samples from individual animals in 11 herds were also taken on three occasions. the prevalence of bhv-1 in the 186 herds was 61% in 2002, decreasing to 48% in 2004 and for bvdv was 91% in 2002, decreasing to 72% in 2004. a b ... | 2009 | 18760643 |
| antimicrobial and cytotoxic arylazoenamines. part iii: antiviral activity of selected classes of arylazoenamines. | eighty-five arylazoenamines, characterized by different types of aryl and basic moieties, have been synthesized and evaluated in cell-based assays for cytotoxicity and antiviral activity against a panel of ten rna and dna viruses. the most commonly affected viruses were, in decreasing order, cvb-2, rsv, bvdv, yfv, and sb-1; the remaining viruses were either not affected (hiv-1, vsv, and vv) or susceptible only to a very few compounds (reo-1 and hsv-1). thirty-five compounds exhibited high activi ... | 2008 | 18760610 |
| antibody prevalence against respiratory viruses in sheep and goats in north-western turkey. | respiratory viruses may infect both small and large ruminant species, and can be transmitted among those of species. present study reports presence and serological distribution of bovine respiratory viral infections in sheep and goats in marmara region of turkey. total of 388 sera, 228 from sheep and 160 from goats collected from 4 provinces were analysed. neutralising antibodies specific to bvdv, bhv-1, brsv, pi-3, bav-1 and bav-3 were investigated. among 388 serum samples 32.1% were positive f ... | 2009 | 18756353 |
| formation of bovine viral diarrhea virus e1-e2 heterodimers is essential for virus entry and depends on charged residues in the transmembrane domains. | the envelope of bovine viral diarrhea virus (bvdv) contains the glycoproteins erns, e1 and e2. complementation of a recombinant vesicular stomatitis virus (vsv) with bvdv glycoproteins resulted in infectious pseudotyped viruses. to elucidate the specific role of each of the single envelope glycoproteins during viral entry, pseudotypes were generated bearing the bvdv envelope proteins in different combinations. pseudoviruses that contained e1 and e2 but not erns were infectious, indicating that e ... | 2008 | 18753220 |
| systemic reactive angioendotheliomatosis-like syndrome in a steer presumed to be persistently infected with bovine viral diarrhea virus. | unusual proliferative intravascular lesions were seen in multiple organs of a 2-year-old corriente steer presumed to be persistently infected with bovine viral diarrhea virus (bvdv), based on widespread immunohistochemical detection of bvdv antigen. proliferations of spindle cells, which were immunohistochemically positive for von willebrand factor-related antigen, partially-to-completely occluded vessel lumens and were supported by cells that were immunohistochemically positive for smooth muscl ... | 2008 | 18725468 |
| outbreak of salmonella enterica serotype newport in a beef cow-calf herd associated with exposure to bovine viral diarrhea virus. | severe disease and death in cows and calves affected 1 of 3 separate groups (a, b, and c) of cattle on a commercial cow-calf operation. | 2008 | 18710321 |
| the antiviral activities of artemisinin and artesunate. | traditional chinese medicine commands a unique position among all traditional medicines because of its 5000 years of history. our own interest in natural products from traditional chinese medicine was triggered in the 1990s, by artemisinin-type sesquiterpene lactones from artemisia annua l. as demonstrated in recent years, this class of compounds has activity against malaria, cancer cells, and schistosomiasis. interestingly, the bioactivity of artemisinin and its semisynthetic derivative artesun ... | 2008 | 18699744 |
| risk factors associated with mycobacterium avium subspecies paratuberculosis seropositivity in canadian dairy cows and herds. | our objective was to determine the risk factors associated with the seroprevalence of mycobacterium avium subspecies paratuberculosis (map) in a large number of randomly selected canadian dairy herds, controlling for important confounding variables and co-infections with bovine leukemia virus (blv), bovine viral diarrhea virus (bvdv) and neospora caninum (nc). serum samples from 30 randomly selected cows, where available, in 315 herds from seven provinces were tested for antibodies against blv, ... | 2009 | 18692926 |
| normal reproductive capacity of heifers that originated from in vitro fertilized embryos cultured with an antiviral compound. | bovine viral diarrhea virus (bvdv) can associate with in vitro fertilized (ivf) bovine embryos despite washing and trypsin treatment. an antiviral compound, db606 (2-(4-[2-imidazolinyl]phenyl)-5-(4-methoxyphenyl)furan), inhibits the replication of bvdv in bovine uterine tubal epithelial cells, madin darby bovine kidney cells, and fetal fibroblast cells. as well, db606 in in vitro culture medium does not affect embryonic development. antiviral-treated-ivf embryos placed into recipients developed ... | 2009 | 18691836 |
| antibodies to selected viral disease agents in wild boars from the czech republic. | blood samples were collected from wild boar (sus scrofa) shot during the hunting season from 1999 to 2005 in the czech republic. sera were tested by enzyme-linked immunosorbent assay for the presence of antibodies against classical swine fever virus (csfv), swine vesicular disease virus (svdv), aujeszky's disease virus (adv), and bovine viral diarrhea virus (bvdv). indirect fluorescence antibody test was used for detection of antibodies against porcine circovirus type 2 (pcv-2) and transmissible ... | 2008 | 18689671 |
| bovine viral diarrhea virus multiorgan infection in two white-tailed deer in southeastern south dakota. | the susceptibility of wild ruminants, especially cervids, to bovine viral diarrhea virus (bvdv) has remained an enigma. two white-tailed deer (odocoileus virginianus) were submitted to the animal disease research and diagnostic laboratory (adrdl) in the fall of 2003 by the south dakota game fish and parks for chronic wasting disease (cwd) testing. both animals were cwd negative. the animals were necropsied and histopathology, viral antigen detection, and virus isolation were performed. a noncyto ... | 2008 | 18689667 |
| the use of sscp analysis [corrected] to evidence genetic variability in the gene coding for immunodominant protein e2 of the bvdv. | 2008 | 18683063 | |
| improvement of virus safety of an antihemophilc factor ix by virus filtration process. | viral safety is an important prerequisite for clinical preparations of plasma-derived pharmaceuticals. one potential way to increase the safety of therapeutic biological products is the use of a virus-retentive filter. in order to increase the viral safety of human antihemophilic factor ix, particularly in regard to non-enveloped viruses, virus removal process using a polyvinylidene fluoride membrane filter (viresolve nfp) has been optimized. the most critical factor affecting the filtration eff ... | 2008 | 18667862 |
| prevalence and risk factors associated with bovine viral diarrhea virus infection in dairy herds in jordan. | a cross-sectional study was carried out to determine the seroprevalence and to identify risk factors associated with bovine viral diarrhea virus (bvdv) infection in 62 non-vaccinated dairy herds (671 cows) in jordan between january and june 2007. information regarding herd management was recorded through a personal interview with farmers. antibodies against bvdv were detected using an indirect elisa test. chi-square analysis and multivariable logistic regression model were used to identify risk ... | 2009 | 18654834 |
| cytopathogenicity of classical swine fever virus correlates with attenuation in the natural host. | for the important livestock pathogens classical swine fever virus (csfv) and bovine viral diarrhea virus (bvdv), cytopathogenic (cp) and non-cp viruses are distinguished according to the induction of apoptosis in infected tissue culture cells. however, it is currently unknown whether cp csfv differs from non-cp csfv with regard to virulence in the acutely infected host. in this study, we generated helper virus-independent csfv alfort-jiv, which encompasses sequences encoding domain jiv-90 of cel ... | 2008 | 18653456 |
| dry-heat treatment process for enhancing viral safety of an antihemophilic factor viii concentrate prepared from human plasma. | viral safety is a prerequisite for manufacturing clinical antihemophilic factor viii concentrates from human plasma. with particular regard to the hepatitis a virus (hav), a terminal dry-heat treatment (100 degrees for 30 min) process, following lyophilization, was developed to improve the virus safety of a solvent/detergent-treated antihemophilic factor viii concentrate. the loss of factor viii activity during dry-heat treatment was of about 5%. no substantial changes were observed in the physi ... | 2008 | 18633304 |
| genoepidemiological evaluation of bovine viral diarrhea virus 2 species based on secondary structures in the 5' untranslated region. | bovine viral diarrhea virus 2 (bvdv-2) strains demonstrated in cattle, sheep, and adventitious contaminants of biological products have been evaluated by the palindromic nucleotide substitutions (pns) method at the three variable loci (v1, v2 and v3) in the 5' untranslated region (utr), to determine their taxonomical status. variation in conserved genomic sequences was used as parameter for epidemiological evaluation of the species in relation with geographical distribution, animal host and viru ... | 2008 | 18628597 |
| availability of oral swab sample for the detection of bovine viral diarrhea virus (bvdv) gene from the cattle persistently infected with bvdv. | bovine nasal and oral discharges were used as samples for bovine viral diarrhea virus (bvdv) gene detection. viral genes in serum (s), nasal discharge (n) and oral discharge (o) were quantified with real-time polymerase chain reaction using sybr green by the relative quantification method, and findings were compared among samples. although the quantity of the bvdv gene in s was greater than those in n and o, all samples were available to identify persistently infected (pi) cattle with bvdv by re ... | 2008 | 18624254 |
| genetic typing of bovine viral diarrhoea virus: evidence of an increasing number of variants in italy. | bovine viral diarrhoea virus (bvdv) is responsible worldwide for severe economic losses on cattle farms. bvdv is an rna virus with a high genome variability having practical consequences on epidemiology, diagnosis and disease control. genetic monitoring was suggested as the first step in bvdv control. thirty-seven bovine viral diarrhoea viruses were identified in persistently infected cattle, mucosal disease-affected animals and in bulk milk, and were characterised genetically. the 5'utr region ... | 2008 | 18623993 |
| antiviral activity of geneticin against bovine viral diarrhoea virus. | aminoglycoside g418 is commonly used to generate stable replicons for rna viruses, such as hepatitis c virus, west nile virus, and bovine viral diarrhoea virus (bvdv). this precludes testing 6418's own antiviral activities against those viruses. here, we report antiviral activity of 6418 against bvdv. | 2008 | 18610556 |
| [experiences with a bvd-free transhumance in the summer of 2006]. | the aim of this paper was to examine the effect of eliminating persistently infected (pi) animals on bvdv infection during transhumance and to identify possible weak points in the prevention of new infection. an initial blood sample (a) was taken from all the animals until one week before the date of trans-humance (n = 190) and examined for virus by means of real-time rt-pcr or antigen-elisa and for antibodies by means of elisa. one pi animal was identified and eliminated. on the day of transhum ... | 2008 | 18605017 |
| effect of age at the time of vaccination on antibody titers and feedlot performance in beef calves. | to compare antibody responses, feedlot morbidity and mortality rates, feedlot performance, and carcass value for calves vaccinated with 1 of 2 vaccination strategies and for unvaccinated control calves. | 2008 | 18593324 |
| associations between health and productivity in cow-calf beef herds and persistent infection with bovine viral diarrhea virus, antibodies against bovine viral diarrhea virus, or antibodies against infectious bovine rhinotracheitis virus in calves. | to measure associations between health and productivity in cow-calf beef herds and persistent infection with bovine viral diarrhea virus (bvdv), antibodies against bvdv, or antibodies against infectious bovine rhinotracheitis (ibr) virus in calves. | 2008 | 18593246 |
| alteration in ultrastructural morphology of bovine embryos following subzonal microinjection of bovine viral diarrhea virus (bvdv). | the aim of the present study was to evaluate the development and ultrastructure of preimplantation bovine embryos that were exposed to bovine viral diarrhea virus (bvdv) in vitro. the embryos were recovered from superovulated and fertilized holstein-friesian donor cows on day 6 of the estrous cycle. compact morulae were microinjected with 20 pl of bvdv suspension (10(5.16) tcid(50)/ml viral stock diluted 1:4) under the zona pellucida (zp), then washed in sof medium and cultured for 24-48 h. embr ... | 2008 | 18578943 |
| bovine viral diarrhea viruses differentially alter the expression of the protein kinases and related proteins affecting the development of infection and anti-viral mechanisms in bovine monocytes. | using a proteomics approach, we evaluated the effect of cytopathic (cp), and non-cytopathic (ncp) bovine viral diarrhea viruses (bvdv) on the expression of protein kinases and related proteins in bovine monocytes. proteins were isolated from membrane and cytosolic fractions with the differential detergent fractionation (ddf) method and identified with 2d-lc esi ms2. of approximately 10,000 proteins identified, 378 proteins had homology with known protein kinases or related proteins. eighteen pro ... | 2008 | 18570900 |
| cleavage of the ns2-3 protein in the cells of cattle persistently infected with non-cytopathogenic bovine viral diarrhea virus. | the ns2-3 of bvdv is cleaved in cultured cells infected with cp bvdv but not in those infected with ncp bvdv when tested more than 10 hours post infection. however, it is not known whether cleavage of ns2-3 occurs in vivo. in the present study, cleavage of ns2-3 in cattle persistently infected with bvdv was investigated. all bvdv isolated from pi animals were of the ncp biotype, and ns2-3 proteins were detected in bovine fetal muscular cells infected with these viruses. on the other hand, in the ... | 2008 | 18557898 |
| generation and characterization of an npro-disrupted marker bovine viral diarrhea virus derived from a bac cdna. | in vitro studies showed that n(pro) protein of bovine viral diarrhea virus (bvdv) interferes with cellular antiviral defense. to understand the role of n(pro) protein in successful viral invasion of the host and establishment of the lifetime persistence, an infectious n(pro)-disrupted virus with a noncytopathic (ncp) background is desired. in this study, an n(pro)-disrupted cdna, pbsd1-n(pro)/egfp2a, was constructed based on an infectious full-length bac cdna clone of ncp bvdv strain sd1, pbsd1. ... | 2008 | 18555541 |
| bvdv in british alpacas. | 2008 | 18552333 | |
| seroprevalence of bovine respiratory viruses in north-western turkey. | bovine respiratory disease complex is a very important health problem around the world. present study describes serological distribution of bovine major respiratory viruses in non-vaccinated cattle population of marmara region in north-western turkey. neutralising antibodies specific to bovine viral diarrhoea virus (bvdv), bovine herpesvirus 1 (bhv-1), bovine respiratory syncytial virus (brsv), bovine parainfluenza virus 3 (pi-3), bovine adenovirus serotype 1 (bav-1) and serotype 3 (bav-3) were ... | 2008 | 18551779 |
| [solvent-detergent viral inactivation of minipools of plasma for transfusion, cryoprecipitate and cryo-poor plasma in single-use bag systems]. | non-virally inactivated plasma, cryoprecipitate and cryoprecipitate-poor plasma, prepared by blood establishments, are still used in many countries in the world, in both the developing world and industrialized countries, for the treatment of various hematological disorders. in the absence of viral inactivation treatment, these fractions may be involved, in spite of increasingly sensitive viral detection methods, into the transmission of plasma-borne viruses, most critically hiv and hepatitis b ( ... | 2008 | 18538608 |
| frequency of detectable serum igg concentrations in precolostral calves. | to determine the prevalence of detectable serum igg concentrations in calves prior to ingestion of colostrum and to assess whether a detectable igg concentration was related to dam parity, calf birth weight, calf sex, season of calving, or infectious agents that can be transmitted transplacentally. | 2008 | 18518660 |
| development of a magnetic bead microarray for simultaneous and simple detection of four pestiviruses. | this study reports a novel method for the rapid detection and identification of the four recognized species in the pestivirus genus of the flaviviridae family, i.e. classical swine fever virus (csfv), border disease virus (bdv), bovine viral diarrhoea virus type 1 (bvdv1) and type 2 (bvdv2). the analysis of pestivirus pcr products was performed on microarrays by means of magnetic bead detection. the process utilizes an oligonucleotide array, onto which 5' biotinylated pcr products were hybridize ... | 2009 | 18514335 |
| microbiological and histopathological findings in cases of fatal bovine respiratory disease of feedlot cattle in western canada. | the aim of this study was to describe the microbiologic agents and pathologic processes in fatal bovine respiratory disease (brd) of feedlot cattle and to investigate associations between agents and pathologic processes. ninety feedlot calves diagnosed at necropsy with brd and 9 control calves without brd were examined, using immunohistochemical (ihc) staining and histopathologic studies. mannheimia haemolytica (mh) (peracute, acute, and subacute cases) and mycoplasma bovis (mb) (subacute, bronc ... | 2008 | 18512458 |
| a comparison of 2 vaccination programs in feedlot calves at ultra-high risk of developing undifferentiated fever/bovine respiratory disease. | the aim of this study was to compare 2 vaccination programs in feedlot calves at ultra-high risk of developing undifferentiated fever (uf)/bovine respiratory disease (brd). at feedlot arrival, 3882 calves were enrolled in the study and randomly allocated to 2 groups, which were housed by group in 12 pens. at the time of allocation, 1 group (mlv3-bt2) received a multivalent, modified-live viral vaccine containing infectious bovine rhinotracheitis virus (ibrv) and types i and ii bovine viral diarr ... | 2008 | 18512457 |
| the fetal brain in bovine viral diarrhea virus-infected calves: lesions, distribution, and cellular heterogeneity of viral antigen at 190 days gestation. | previous studies have shown that the brain is a target of persistent infection with bovine viral diarrhea virus (bvdv) and have demonstrated viral tropism for neurons as well as other endogenous cell types in diverse brain areas. apart from foci of mild residual inflammation in some postnatal calves, consistent brain lesions, per se, have not been reported. no similar comprehensive studies of the brain have been reported in bovine fetuses. in the current study, 12 bvdv-seronegative heifers were ... | 2008 | 18487484 |
| application of restriction fragment length polymorphism analysis to simple and rapid genotyping of bovine viral diarrhea virus strains isolated in japan. | the e2 regions of 177 bovine viral diarrhea virus (bvdv) strains isolated in japan between 1957 and 2006 were analyzed for genotyping. the strains were classified into 8 genotypes (1a, 1b, 1c, 1d, 1e, 1f, so and 2a) based on the phylogenetic analysis. the restriction fragment length polymorphism (rflp) analysis of the rt-pcr products using 6 selected enzymes (apo i, mly i, bstap i, pvu ii, ear i, ecor v) disclosed the cutting patterns classified into 11 groups (i-xi), each of that consisted of s ... | 2008 | 18460835 |
| histopathologic and immunohistochemical findings in two white-tailed deer fawns persistently infected with bovine viral diarrhea virus. | bovine viral diarrhea virus (bvdv) is an important pathogen of domestic cattle. serologic, experimental, and individual case studies explored the presence and pathogenesis of the virus in wild ungulates; however, there remain large gaps in knowledge regarding bvdv infection in nonbovine species. live twins were born from a white-tailed deer (odocoileus virginianus) doe infected with noncytopathic bvdv during its first trimester of pregnancy. the twins died at 1 day of age from trauma unrelated t ... | 2008 | 18460614 |
| development of a duplex quantitative polymerase chain reaction assay for detection of bovine herpesvirus 1 and bovine viral diarrhea virus in bovine follicular fluid. | the objective of this study was to develop a duplex quantitative polymerase chain reaction (qpcr) assay for simultaneous detection of bovine herpesvirus 1 (bohv-1) and bovine viral diarrhea virus (bvdv) type i and type ii. follicular fluid was collected from a bohv-1 acutely infected heifer, a bvdv i persistently infected heifer, and from 10 ovaries recovered from an abattoir. both the bohv-1 and bvdv contaminated follicular fluid were diluted 1:5 to 1:10(7) using the pooled, abattoir-origin fol ... | 2008 | 18452983 |
| increase in proto-oncogene mrna transcript levels in bovine lymphoid cells infected with a cytopathic type 2 bovine viral diarrhea virus. | infection of susceptible animals with bovine viral diarrhea viruses (bvdv) can result in an array of disease symptoms that are dependent in part on the strain of infecting virus and the physiological status of the host. bvdv are lymphotrophic and exist as two biotypes. cytopathic bvdv kill cells outright while noncytopathic strains can readily establish persistent infections. the molecular mechanisms behind these different affects are unknown. to gain a better understanding of the mechanisms of ... | 2008 | 18440085 |
| experimental superinfection of a lesser malayan mousedeer (tragulus javanicus) persistently infected with bovine viral diarrhea virus. | a lesser malayan mousedeer (tragulus javanicus), persistently infected with noncytopathogenic bovine viral diarrhea virus (bvdv) type 1f, was experimentally superinfected with a cytopathogenic isolate of bvdv type 1c, which antigenically partially matched the endogenous strain. within the observational period of 125 days after superinfection, the animal did not demonstrate any clinical signs of the disease and/or significant changes in blood values. neutralizing antibodies were detected at 35 an ... | 2008 | 18432109 |