Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| the african swine fever virus dp71l protein recruits the protein phosphatase 1 catalytic subunit to dephosphorylate eif2alpha and inhibits chop induction but is dispensable for these activities during virus infection. | the african swine fever virus (asfv) dp71l protein is present in all isolates as either a short form of 70 to 72 amino acids or a long form of about 184 amino acids, and both of these share sequence similarity to the c-terminal domain of the herpes simplex virus icp34.5 protein and cellular protein gadd34. in the present study we expressed dp71l in different mammalian cells and demonstrated that dp71l causes dephosphorylation of eukaryotic translation initiation factor 2 alpha (eif2α) in resting ... | 2010 | 20702639 |
| characterization of essential domains and plasticity of the classical swine fever virus core protein. | pestiviruses are pathogens of cloven-hoofed animals, belonging to the flaviviridae. the pestiviral particle consists of a lipid membrane containing the three envelope glycoproteins erns, e1, and e2 and a nucleocapsid of unknown symmetry, which is composed of the core protein and the viral positive-sense rna genome. the positively charged pestiviral core protein consists of 86 to 89 amino acids. to analyze the organization of essential domains, n- and c-terminal truncations, as well as internal d ... | 2010 | 20702631 |
| establishment of a multiplex rt-pcr assay to detect different lineages of swine h1 and h3 influenza a viruses. | classical swine h1n1, emerging european avian-like h1n1 and human-like h3n2 lineages are co-circulating in the swine population in china. the reverse transcriptase polymerase chain reaction (rt-pcr) assay is an effective method for use in influenza surveillance. in this study, a multiplex rt-pcr method was developed for simultaneous identification of hemagglutinin (ha) genes derived from the three lineages of swine influenza viruses. three primer sets were designed and aimed specifically at ha g ... | 2010 | 20700759 |
| loop-mediated isothermal amplification for rapid detection and differentiation of wild-type pseudorabies and gene-deleted virus vaccines. | a loop-mediated isothermal amplification (lamp) assay was developed specifically for detection and differentiation of pseudorabies virus (prv). one group of primers was designed to detect wild-type strains (i.e., strains with the ge gene) and the other group of primers was designed to detect both prv ge-vaccine and wild-type strains (i.e., strains with the gg gene and with or without the ge gene). after amplification by bst enzyme at a constant temperature of 65 degrees c, a laddering of bright ... | 2010 | 20691214 |
| in vitro inhibition of csfv replication by retroviral vector-mediated rna interference. | classical swine fever (csf) is a highly contagious viral disease of pigs which causes major economic losses worldwide. no specific drug is currently available for the effective treatment of csfv infection. rna interference (rnai) technology depends on effective delivery systems, for which several effective vectors have recently been developed. three retroviral plasmids containing sirna genes targeting different regions of n(pro) and ns4a have been constructed, and 3 replication-incompetent retro ... | 2010 | 20691206 |
| small peptide inhibitors disrupt a high-affinity interaction between cytoplasmic dynein and a viral cargo protein. | several viruses target the microtubular motor system in early stages of the viral life cycle. african swine fever virus (asfv) protein p54 hijacks the microtubule-dependent transport by interaction with a dynein light chain (dynll1/dlc8). this was shown to be a high-affinity interaction, and the residues gradually disappearing were mapped on dlc8 to define a putative p54 binding surface by nuclear magnetic resonance (nmr) spectroscopy. the potential of short peptides targeting the binding domain ... | 2010 | 20686048 |
| transmission of classical swine fever virus depends on the clinical course of infection which is associated with high and low levels of virus excretion. | infection with moderately virulent strains of classical swine fever virus (csfv) can lead to different courses of disease: either (sub)acute, resulting in death or recovery, or chronic disease. the virus excretion dynamics between these courses are quite dissimilar, but it is not known if this also results in differences in virus transmission. in this study, the excretion and transmission dynamics of the moderately virulent paderborn strain were studied in 15 one-to-one experiments. in these exp ... | 2011 | 20674195 |
| molecular epidemiology of current classical swine fever virus isolates of wild boar in germany. | classical swine fever (csf) has caused significant economic losses in industrialized pig production, and is still present in some european countries. recent csf outbreaks in europe were mainly associated with strains of genogroup 2 (subgroup 2.3). although there are extensive datasets regarding 2.3 strains, there is very little information available on longer fragments or whole classical swine fever virus (csfv) genomes. furthermore, there are no detailed analyses of the molecular epidemiology o ... | 2010 | 20660149 |
| complete genomic sequence of a border disease virus isolated from pyrenean chamois. | this report describes the full-length genome sequence of the pestivirus strain h2121 which was recently isolated from pyrenean chamois and typed as border disease virus (bdv) genotype 4. comparison with full-length genomic sequences of the approved pestivirus species bovine viral diarrhea virus-1 (bvdv-1), bvdv-2, bdv, and classical swine fever virus, the tentative species represented by strain giraffe-1, as well as the atypical pestivirus strain th/04_khonkaen confirmed that the chamois pestivi ... | 2010 | 20638945 |
| classical swine fever virus ns3 is an ires-binding protein and increases ires-dependent translation. | to get more evidences for understanding the role of ns3 in viral translation, we observed the promotive effect of csfv ns3 on ires-mediated translation by using dicistronic and monocistronic systems containing the precise segment comprising csfv ires. the results for affinity chromatography and uv-crosslinking assays indicated that ns3 bound csfv ires and that csfv ns5a and ns5b could reduce the ires-ns3 interaction. further experiments showed that the ns5a also bound the ires and that ns3 and n ... | 2010 | 20637813 |
| the classical swine fever virus n-terminal protease n(pro) binds to cellular hax-1. | the positive-stranded rna genome of classical swine fever virus (csfv) encodes 12 known proteins. the first protein to be translated is the n-terminal protease (n(pro)). n(pro) helps evade the innate interferon response by targeting interferon regulatory factor-3 for proteasomal degradation and also participates in the evasion of dsrna-induced apoptosis. to elucidate the mechanisms by which n(pro) functions, we performed a yeast two-hybrid screen in which the anti-apoptotic protein hax-1 was ide ... | 2010 | 20631090 |
| expression and immunological studies of classical swine fever virus glycoprotein e2 in the bi-cistronic baculovirus/larvae expression system. | to develop an economical, easy technique for producing recombinant e2 glycoprotein (re2) of classical swine fever virus (csfv) as a candidate immunogen, a bi-cistronic baculovirus/larvae expression vector was constructed using p10 promoter, an internal ribosome entry site, and the gfp gene. trichoplusia ni larvae were successfully infected with the occluded recombinant baculovirus via feed, and the characteristics of re2 were confirmed by immunoblot and glycosylation stain. re2 at a concentratio ... | 2010 | 20622456 |
| sensitive detection of african swine fever virus using real-time pcr with a 5' conjugated minor groove binder probe. | the design of a 5' conjugated minor groove binder (mgb) probe real-time pcr assay is described for the rapid, sensitive and specific detection of african swine fever virus (asfv) dna. the assay is designed against the 9gl region and is capable of detecting 20 copies of a dna standard. it does not detect any of the other common swine dna viruses tested in this study. the assay can detect asfv dna in a range of clinical samples. sensitivity was equivalent to the office international des epizooties ... | 2010 | 20621646 |
| proteomic analysis of primary porcine endothelial cells after infection by classical swine fever virus. | endothelial cells are the main target of classical swine fever virus during infection, and extensive hemorrhage is the most typical clinical sign of classical swine fever. to investigate the molecular mechanism of hemorrhagic pathogenesis, two-dimensional difference gel electrophoresis with fluorescent dyes (2d-dige) was used to analyze the proteomic profile of primary porcine umbilical vein endothelial cells (puvecs) following csfv infection. of 15 protein spots with differential expression, 8 ... | 2010 | 20595071 |
| improved sero-monitoring assay for classical swine fever (csf) using the recombinant e2 protein of a recent korean isolate. | classical swine fever (csf) is a highly contagious disease of pigs that causes fever, diarrhea and paralysis, often resulting in death. e2 is the major structural protein of the csf virus (csfv) and mediates the entrance of the virus, subsequently inducing a neutralizing immune response. in this study, the e2 gene of a recent korean isolate of csf, sw03, was cloned and the dna sequence was compared to other strains via phylogenetic analysis. with the purified e2 protein, an enzyme-linked immunos ... | 2011 | 20591455 |
| identification of antigen-specific residues on e2 glycoprotein of classical swine fever virus. | envelope glycoprotein e2 of classical swine fever virus (csfv) is the major antigen that induces neutralizing antibodies in infected pigs. our previous study revealed that n-terminal 90 residues (domains b/c) of e2 play key roles in differentiating vaccine strain lpc/ahri (subgroup 1.1) from the two field strains td/96/twn (subgroup 2.1) and 94.4/il/94/twn (subgroup 3.4) (chang et al., 2010). this study further analyzed the reaction patterns between monoclonal antibodies (mabs) and expressed hyb ... | 2010 | 20558217 |
| understanding inhibition of viral proteins on type i ifn signaling pathways with modeling and optimization. | the interferon system provides a powerful and universal intracellular defense mechanism against viruses. as one part of their survival strategies, many viruses have evolved mechanisms to counteract the host type i interferon (ifn-alpha/beta) responses. in this study, we attempt to investigate virus- and double-strand rna (dsrna)-triggered type i ifn signaling pathways and understand the inhibition of ifn-alpha/beta induction by viral proteins using mathematical modeling and quantitative analysis ... | 2010 | 20553733 |
| phylogenetic analysis of classical swine fever virus isolates from peru. | classical swine fever (csf) is considered to be endemic in peru with outbreaks reported to the world organization for animal health as recently as 2008 and 2009. nevertheless, little is known regarding the genetic subgroup(s) of csf virus that are circulating in peru or their relationship to recent csf viruses that have been isolated from neighbouring south american countries or other parts of the world. in this study, we molecularly characterize csf viruses that were isolated from domestic pigs ... | 2010 | 20545910 |
| crystal structure and functional characterization of an immunomodulatory salivary cystatin from the soft tick ornithodoros moubata. | the saliva of blood-feeding parasites is a rich source of peptidase inhibitors that help to overcome the host's defence during host-parasite interactions. using proteomic analysis, the cystatin omc2 was demonstrated in the saliva of the soft tick ornithodoros moubata, an important disease vector transmitting african swine fever virus and the spirochaete borrelia duttoni. a structural, biochemical and biological characterization of this peptidase inhibitor was undertaken in the present study. rec ... | 2010 | 20545626 |
| classical swine fever virus in south-eastern europe--retrospective analysis of the disease situation and molecular epidemiology. | classical swine fever (csf) is among the most important diseases of domestic pigs and causes great socio-economic losses. therefore, control of csf is given high priority within the european union, including financial support of concerted control actions in candidate and in potential candidate countries. unfortunately, from some of these countries information on the csf situation and related data is very limited. this study was undertaken to gather all available information on the domestic pig p ... | 2010 | 20541876 |
| protective efficacy of a classical swine fever virus c-strain deletion mutant and ability to differentiate infected from vaccinated animals. | classical swine fever (csf) continues to be the most economically damaging pig disease in the world. the disease can be effectively controlled by vaccination with the live c-strain vaccine. this vaccine, however, does not enable the serological differentiation between infected and vaccinated animals (diva) and its use can therefore impose severe trade restrictions. csf-specific diagnostic elisas detect antibodies directed against the conserved and immunodominant a domain of the e2 structural gly ... | 2011 | 20541334 |
| [sequence analysis, expression and antigenicity detection of bovine viral diarrhea virus ns3 gene]. | in this study, we cloned the ns3 gene from bovine viral diarrhea virus (bvdv) vedevac strain. the result showed that the average p-distance of pestivirus ns3 amino acid sequence was 0.07 and the vedevac strain was classified to bvdv type 1. using pet-30a(+) as vector and escherichia coli rosetta (de3) as host, we obtained purified recombinant ns3 protein by ni-nta affinity chromatography. western blotting analysis demonstrated that both bvdv positive serum and classical swine fever virus (csfv) ... | 2010 | 20518342 |
| [progress in dna vaccines against classical swine fever: a review]. | in 1990, it was reported that the naked dna encoding an antigen (so-called dna vaccine) transduced directly into the muscle is able to induce immune responses just like antigen inoculation. since then, a number of dna vaccines against different diseases have been developed and shown to induce different levels of specific humoral and/or cell-mediated immunity. efforts have been made to develop effective dna vaccines against classical swine fever (csf). this review covered the following aspects in ... | 2010 | 20518338 |
| expression and purification of antimicrobial peptide cm4 by npro fusion technology in e. coli. | antimicrobial peptide cm4 is a small cationic peptide with broad-spectrum activities against bacteria, fungi, and tumor cells. different strategies have been developed to produce small antibacterial peptides using recombinant techniques. to date, no efforts to obtain large quantities of active recombinant cm4 have been reported. in order to establish a bacterium-based cm4 production system, cm4 was cloned into pet28a and expressed with npro mutant (eddie) fusion. cm4 expressed as eddie are depos ... | 2010 | 20512388 |
| african swine fever virus protein p17 is essential for the progression of viral membrane precursors toward icosahedral intermediates. | the first morphological evidence of african swine fever virus (asfv) assembly is the appearance of precursor viral membranes, thought to derive from the endoplasmic reticulum, within the assembly sites. we have shown previously that protein p54, a viral structural integral membrane protein, is essential for the generation of the viral precursor membranes. in this report, we study the role of protein p17, an abundant transmembrane protein localized at the viral internal envelope, in these process ... | 2010 | 20504920 |
| classical swine fever virus down-regulates endothelial connexin 43 gap junctions. | classical swine fever is a contagious disease of pigs characterized by fatal hemorrhagic fever. classical swine fever virus (csfv) induces the expression of pro-inflammatory and pro-coagulant factors of vascular endothelial cells and establishes a long-term infection. this study aimed to understand the effect of csfv on endothelial connexin 43 (cx43) expression and gap junctional intercellular coupling (gjic). porcine aortic endothelial cells were infected with csfv at different multiplicity of ... | 2010 | 20473696 |
| evaluation of a primer-probe energy transfer real-time pcr assay for detection of classical swine fever virus. | this study describes evaluation of a real-time pcr assay based on primer-probe energy transfer (priproet) technology for detection of classical swine fever virus (csfv). the priproet technology allows melting curve analysis following pcr amplification and thus provides a higher specificity. the assay was compared with a taqman assay by testing a total of 203 samples including 175 clinical specimens and 28 batches of hog cholera lapinized virus (hclv) vaccine. the two assays gave the same results ... | 2010 | 20471428 |
| the toll-like receptor adaptor molecule trif enhances dna vaccination against classical swine fever. | to evaluate the effects of the toll/interleukin-1 receptor domain-containing adaptor-inducing interferon-beta (trif) on immune responses induced by dna vaccines, mice were immunized with the eukaryotic expression plasmid pcdna/e2 encoding classical swine fever virus (csfv) e2 alone or in combination with the trif genetic adjuvant. immune responses were examined in immunized mice. our data demonstrates that co-delivery of the dna vaccine pcdna/e2 with the trif adjuvant augmented specific humoral ... | 2010 | 20466439 |
| changes in the porcine peripheral blood mononuclear cell proteome induced by infection with highly virulent classical swine fever virus. | leukopenia and immunosuppression are characteristic clinical manifestations of classical swine fever and peripheral blood mononuclear cells (pbmcs) are major targets of classical swine fever virus. to investigate proteomic expression changes in swine pbmcs during lethal csfv infection, proteins of pbmcs from five lethally csfv-infected pigs were resolved by two-dimensional electrophoresis followed by mass spectrometry. quantitative intensity analysis revealed that 66 protein spots showed altered ... | 2010 | 20463149 |
| assessment of international inter-laboratory comparison tests for the diagnosis of classical swine fever from 1998 until 2007. | the inter-laboratory comparison tests for classical swine fever (csf) laboratory diagnosis organised by the european community reference laboratory for csf are regularly performed within european union member states. the objective of this study was to evaluate the results of the inter-laboratory comparison tests carried out over the last decade, from 1998 until 2007, by using a statistical approach. a set of five or six lyophilised sera was sent to participants. these included sera containing cs ... | 2009 | 20462168 |
| optimisation of reverse transcription can improve the sensitivity of rt-pcr for detection of classical swine fever virus. | classical swine fever is a highly contagious, notifiable disease of pigs and wild boars listed by the world organisation for animal health (oie). therefore, methods employed in the diagnosis of csf should be fast, sensitive and specific. the aim of this study was optimisation of the reverse transcription reaction to increase the sensitivity of real-time rt-pcr for the detection of classical swine fever virus, the aetiological agent of the disease. the efficiency of reverse transcription reaction ... | 2010 | 20460224 |
| the n-glycosylation of classical swine fever virus e2 glycoprotein extracellular domain expressed in the milk of goat. | classical swine fever virus (csfv) outer surface e2 glycoprotein represents an important target to induce protective immunization during infection but the influence of n-glycosylation pattern in antigenicity is yet unclear. in the present work, the n-glycosylation of the e2-csfv extracellular domain expressed in goat milk was determined. enzymatic n-glycans releasing, 2-aminobenzamide (2ab) labeling, weak anion-exchange and normal-phase hplc combined with exoglycosidase digestions and mass spect ... | 2010 | 20460099 |
| the development of a rapid sybr one step real-time rt-pcr for detection of porcine reproductive and respiratory syndrome virus. | prompt detection of prrsv in the field samples is important for effective prrs control, thereby reducing the potentially serious economic damage which can result from an outbreak. in this study, a rapid sybr-based, one step real-time rt-pcr quantitative reverse transcription pcr (qrt-pcr) has been developed for the detection of porcine reproductive and respiratory syndrome virus (prrsv). primers were designed based on the sequence of highly conservative region of prrsv n gene. | 2010 | 20459705 |
| hypomyelination associated with bovine viral diarrhea virus type 2 infection in a longhorn calf. | a newborn longhorn heifer calf presented with generalized tremors, muscle fasciculations, ataxia, and nystagmus. at necropsy, no gross central nervous system lesions were observed. histologically, the brain and spinal cord had mild to moderate diffuse microgliosis and astrocytosis, minimal nonsuppurative encephalitis, and decreased myelin staining. ultrastructural examination revealed thinning and absence of myelin sheaths. various cell types were immunohistochemically positive for bovine viral ... | 2010 | 20448278 |
| a study on the applicability of implantable microchip transponders for body temperature measurements in pigs. | the applicability of an electronic monitoring system using microchip transponders for measurement of body temperatures was tested in 6-week-old conventional danish weaners infected with classical swine fever virus (csfv). subcutaneous tissue temperatures obtained by the implantable transponders were compared with rectal temperatures, recorded by a conventional digital thermometer. | 2010 | 20444254 |
| enhanced immunity against classical swine fever in pigs induced by prime-boost immunization using an alphavirus replicon-vectored dna vaccine and a recombinant adenovirus. | classical swine fever (csf) - caused by the classical swine fever virus (csfv) - is a fatal disease of pigs that is responsible for extensive losses to the swine industry worldwide. we had demonstrated previously that a prime-boost vaccination strategy using an alphavirus (semliki forest virus, sfv) replicon-vectored dna vaccine (psfv1cs-e2) and a recombinant adenovirus (radv-e2) expressing the e2 glycoprotein of csfv induced enhanced immune responses in a mouse model. in this study, we evaluate ... | 2010 | 20435352 |
| intranuclear detection of african swine fever virus dna in several cell types from formalin-fixed and paraffin-embedded tissues using a new in situ hybridisation protocol. | in this study, a new in situ hybridisation (ish) protocol has been developed to identify african swine fever virus (asfv) genome in formalin-fixed, paraffin-embedded tissues. different digoxigenin labelled asfv-probes were tested, including single asfv-specific oligonucleotides, an 18.5kb restriction fragment from the viral genome and the entire asfv genome. the latter showed the highest sensitivity in all tissues tested, independently of the virus used for challenge: e75l or ba71l. although a s ... | 2010 | 20417663 |
| a one-step real-time reverse transcription-polymerase chain reaction detection of classical swine fever virus using a minor groove binding probe. | the aim of this study was to develop a one-step real-time reverse transcription-polymerase chain reaction assay using the minor groove binding probe (mgb rrt-pcr) for rapid and quantitative detection of classical swine fever virus (csfv). the method, which targets the 5'-nontranslated region (5'ntr) of the viral genome, detected all csfv isolate tested, but not heterologous pathogens. using an in vitro transcript of the 5'ntr as a quantitative standard for the csfv genome copy number, the assay ... | 2010 | 20411415 |
| genetic variation of classical swine fever virus based on palindromic nucleotide substitutions, a genetic marker in the 5' untranslated region of rna. | forty-three strains of classical swine fever (hog cholera) virus (csfv) from outbreaks in pigs in europe, asia and america, two strains from commercial csfv modified live vaccines and a strain isolated from a diseased lamb from spain were subjected to analyses of nucleotide sequence variations in the 5' terminal region of the genome. these isolates were divided into three clusters, namely: csfv-1, csfv-2, and csfv-3, based on palindromic nucleotide substitutions in the 5' untranslated region (ut ... | 2008 | 20405435 |
| an rna-hydrolyzing recombinant antibody exhibits an antiviral activity against classical swine fever virus. | some proteins with ribonuclease (rnase) activity have been shown to suppress viral replication. a well-characterized recombinant antibody, 3d8 single-chain variable fragment (3d8 scfv), has rna-hydrolyzing and cell-penetrating activities. here, we investigated antiviral activity of 3d8 scfv against classical swine fever virus (csfv). in a cell line expressing 3d8 scfv (c26), intracellular rna-hydrolysis activity was higher compared to control pk-15 cells and viral replication was strongly suppre ... | 2010 | 20382124 |
| isolation and characterization of the first chinese strain of porcine teschovirus-8. | investigations were carried out to identify the causal agent of acute diarrhea, respiratory distress, and death of pigs on a swine farm in jilin province, northern china. only porcine teschovirus (ptv, designated as ptv-8 jilin/2003) was isolated from samples of organs. the presence of ptv was confirmed by the production of a specific cytopathic effect on susceptible cells and by the results of the immunoperoxidase monolayer assay (ipma), polymerase chain reaction, and electron microscopy. other ... | 2010 | 20362007 |
| [preparation and bioactivity of anti-human red blood cell scfv and csfv e2 bifunctional fusion protein]. | the aim of this study is to construct a bifunctional fusion protein, which can conjugate both human red blood cells and antibodies against classical swine fever virus (csfv). we respectively amplified 2e8scfv and me2 genes from different recombinant vectors, in which 2e8scfv gene is the single chain fv gene against h antigen of human red blood cells, whereas me2 gene is the main antigen coding region gene of csfv e2 protein. we used overlap extension pcr to obtain an artificial fusion gene segme ... | 2010 | 20353089 |
| escape of classical swine fever c-strain vaccine virus from detection by c-strain specific real-time rt-pcr caused by a point mutation in the primer-binding site. | classical swine fever (csf) is one of the most important diseases of pigs. vaccination in the european union is limited to emergency situations. currently, vaccination for the purpose of disease control is carried out in wild boar populations. wild boar are in most cases vaccinated using an oral bait vaccine based on the live modified vaccine virus c-strain "riems". a real-time reverse transcription polymerase chain reaction (rt-pcr) protocol for differentiation of c-strain "riems" vaccine virus ... | 2010 | 20332004 |
| inhibition of replication of classical swine fever virus in a stable cell line by the viral capsid and staphylococcus aureus nuclease fusion protein. | classical swine fever (csf) is one of the major diseases causing serious economic losses to the swine industry. to explore the feasibility of using capsid-targeted viral inactivation (ctvi) as an antiviral strategy against csf infection, a plasmid pcdna-cap-snase was constructed for expressing a fusion protein of csfv capsid (cap) and staphylococcus aureus nuclease (snase). under g418 selection, a mammalian cell line pk-15 expressing stably the fusion protein cap-snase(pk-15/cap-snase) could be ... | 2010 | 20304012 |
| validation of a loop-mediated isothermal amplification assay for visualised detection of wild-type classical swine fever virus. | loop-mediated isothermal amplification (lamp) is a nucleic acid amplification method applied and adapted to the detection of a number of pathogens. a lamp assay was developed for the specific detection of wild-type classical swine fever virus (csfv). based on an alignment of genomic sequences of pestiviruses available in genbank, four primers were selected targeting six positions in the ns5b gene region of the viral genome. the assay was performed in a simple water bath at a constant temperature ... | 2010 | 20304010 |
| patterns of gene expression in swine macrophages infected with classical swine fever virus detected by microarray. | infection of domestic swine with highly virulent, classical swine fever virus (csfv) strain brescia, causes lethal disease in all infected animals. however, the molecular mechanisms involved in modulating the host cellular processes and evasion of the immune response have not been clearly established. to gain insight into, the early host response to csfv, we analyzed the pattern of gene expression in infected swine macrophages, using custom designed swine microarrays. macrophages, the target cel ... | 2010 | 20302897 |
| screwworms, cochliomyia hominivorax, reared for mass release do not carry and spread foot-and-mouth disease virus and classical swine fever virus. | experiments were done to determine if transporting live screwworms cochliomyia hominivorax coquerel (diptera: calliphoridae) for developing new strains from countries where foot-and-mouth disease and classical swine fever are endemic, to the mass rearing facilities in mexico and panama, may introduce these exotic diseases into these countries. are screwworms capable of harboring and spreading foot-and-mouth disease virus (fmdv) and classical swine fever virus (csfv) when they are grown in virus- ... | 2008 | 20302530 |
| serial passage of hog cholera virus in rabbits. | 1946 | 20274311 | |
| propagation of hog cholera virus in rabbits. | 1946 | 20274310 | |
| genetic characterization of e2 gene of classical swine fever virus by restriction fragment length polymorphism and phylogenetic analysis. | an rt-nested pcr (rt-npcr)-based restriction fragment length polymorphism (rflp) analyses of the e2 gene were developed for genetic subtyping and differentiation of vaccinated and infected classical swine fever virus (csfv) strains. rt-npcr identified 96 csfv-positive samples from 321 clinical specimens from southeastern china during 2003-2008. the pcr products of positive samples were further differentiated using mspi digestion, 23 were identified as the c-strain, 62 as field strains, and 11 as ... | 2010 | 20217206 |
| the development of oral fluid-based diagnostics and applications in veterinary medicine. | the purpose of this review was to discuss the history of the development and implementation of oral fluid diagnostics for infectious diseases of humans and domestic animals. the use of oral fluid for the assessment of health and diagnosis of disease in humans and animals has a surprisingly long history. as early as 1909, pollaci and ceraulo reported sensitive and specific agglutination of 'micrococcus melitensis' (brucella melitensis) by oral fluid from patients diagnosed with malta fever. a 198 ... | 2010 | 20202287 |
| 5'-utr-based phylogenetic analysis of classical swine fever virus isolates from india. | classical swine fever (csf) caused by classical swine fever virus (csfv) is a globally significant disease of pigs. genetic typing of csfv isolates can help in understanding the epidemiology of disease and trace down the source of outbreak. 5'-utr sequence analysis and subsequent genetic classification of nine csfv field isolates from india indicated that 3 isolates belonged to genotype 2.1 and were closely related to european csfv strains. the remaining 6 isolates belonged to genotype 1 that co ... | 2010 | 20201618 |
| phylogenomic analysis of 11 complete african swine fever virus genome sequences. | viral molecular epidemiology has traditionally analyzed variation in single genes. whole genome phylogenetic analysis of 123 concatenated genes from 11 asfv genomes, including e75, a newly sequenced virulent isolate from spain, identified two clusters. one contained south african isolates from ticks and warthog, suggesting derivation from a sylvatic transmission cycle. the second contained isolates from west africa and the iberian peninsula. two isolates, from kenya and malawi, were outliers. of ... | 2010 | 20171711 |
| in vitro antiviral activity of some uridine derivatives of 2-deoxy sugars against classical swine fever virus. | classical swine fever virus glycoproteins: e2, e(rns) (e0) and e1 are detected on the external part of viral particles and play a major role in the initial stages of viral infection. they form heterodimeric and homodimeric complexes needed to effectively infect host cells. some glycosylation inhibitors, such as tunicamycin, which act at the early stages of glycan chain processing, can influence, not only glycosylation, but also the stability of e2 and e(rns) glycoproteins, effectively inhibiting ... | 2010 | 20153775 |
| antigenic domains analysis of classical swine fever virus e2 glycoprotein by mutagenesis and conformation-dependent monoclonal antibodies. | glycoprotein e2 of classical swine fever virus (csfv) is the major antigenic protein exposed on the outer surface of the virion that induces main neutralizing antibodies during infection in pigs. this study displays the differences in antigenicity of e2 between vaccine and field strains of csfv by their variable reaction patterns between expressed proteins and monoclonal antibodies (mabs). the d/a domains of various csfvs were relatively conserved and recognized by all mabs against the a domain. ... | 2010 | 20132851 |
| [applications of reverse genetics in studying classical swine fever virus]. | classical swine fever (csf), caused by classical swine fever virus (csfv), has been epidemic or endemic in many countries, and causes great economical losses to pig industry worldwide. attenuated vaccines (such as c-strain) have played an important role in the control of csf. recently some new phenomena appear, such as atypical and persistent infections of csf, immunization failure and so on. meanwhile, eradication programs have been implemented in many countries, restricting the widespread appl ... | 2009 | 20112686 |
| prevalence of antibodies to selected viral and bacterial pathogens in wild boar (sus scrofa) in campania region, italy. | serum samples were collected from wild boars (sus scrofa) harvested during the 2005-2006 hunting season in campania, southern italy. samples were tested for antibodies to leptospira interrogan, brucella spp., salmonella spp., aujeszky disease virus (adv), porcine reproductive and respiratory stress syndrome virus (prrsv), porcine parvovirus (ppv), classical swine fever virus (csfv), and swine vesicular disease virus (svdv). of the 342 serum samples tested, 15 (4.4%) were seropositive to brucella ... | 2010 | 20090052 |
| classic swine fever virus ns2 protein leads to the induction of cell cycle arrest at s-phase and endoplasmic reticulum stress. | classical swine fever (csf) caused by virulent strains of classical swine fever virus (csfv) is a haemorrhagic disease of pigs, characterized by disseminated intravascular coagulation, thrombocytopoenia and immunosuppression, and the swine endothelial vascular cell is one of the csfv target cells. in this report, we investigated the previously unknown subcellular localization and function of csfv ns2 protein by examining its effects on cell growth and cell cycle progression. | 2010 | 20064240 |
| molecular characterisation of african swine fever viruses from nigeria (2003-2006) recovers multiple virus variants and reaffirms cvr epidemiological utility. | samples collected from wild and domestic suids in nigeria, over a 3-year period (2003-2006), were evaluated for african swine fever (asf) virus genome presence by targeting three discrete genome regions, namely the 478-bp c-terminal p72 gene region advocated for genotype assignment, a 780-bp region spanning the 5'-ends of the pb125r and pb646l (p72) genes and the hypervariable central variable region (cvr) encoded within the 9rl orf (pb602l). asf virus (asfv) presence was confirmed in 23 of the ... | 2010 | 20052526 |
| correlation of the virulence of csfv with evolutionary patterns of e2 glycoprotein. | infection with classical swine fever virus (csfv) is costly to the livestock industry. several genomic sequences including velogenic strains and low virulent strains have been identified. however, the reasons for the virulence of the virus have remained unclear. based on selective pattern and pressure strength, we classified all genes of csfv into three classes. among these genes, the e2 gene was under the strongest positive selection. based on the analysis of 85 representative e2 gene sequences ... | 2010 | 20036871 |
| global transcriptional profiles in peripheral blood mononuclear cell during classical swine fever virus infection. | classical swine fever virus (csfv) is an etiologic agent that causes a highly contagious disease in pigs. laying a foundation to solve problems in its pathogenic mechanism, microarray analysis was performed to detect the gene transcriptional profiles in peripheral blood mononuclear cells (pbmc) following infection with a chinese highly virulent csfv strain shimen. three susceptible pigs were inoculated intramuscularly with a lethal dose (1.0x10(6) tcid(50)) of csfv. pigs showed classical csf sig ... | 2010 | 20034523 |
| first report of porcine circovirus type 2 infections in cuba. | to obtain information about the porcine circovirus type 2 (pcv2) infection status of pigs in cuba and the probable association of pcv2 with other porcine viruses, tissue samples collected from ill pigs were evaluated using polymerase chain reaction (pcr). the pcr analysis showed that 67.7% of the samples (23/34) from seven swine herds of six different geographic regions were detected to be positive for pcv2. ten of the 23 pcv2 positive samples (43.5%) shown a concurrent infection with porcine pa ... | 2010 | 20031180 |
| additive-multiplicative regression models for spatio-temporal epidemics. | an extension of the stochastic susceptible-infectious-recovered (sir) model is proposed in order to accommodate a regression context for modelling infectious disease data. the proposal is based on a multivariate counting process specified by conditional intensities, which contain an additive epidemic component and a multiplicative endemic component. this allows the analysis of endemic infectious diseases by quantifying risk factors for infection by external sources in addition to infective conta ... | 2009 | 20029897 |
| classical swine fever virus infection protects aortic endothelial cells from pipc-mediated apoptosis. | classical swine fever virus (csfv) causes severe disease in pigs associated with leukopenia, haemorrhage and fever. we show that csfv infection protects endothelial cells from apoptosis induced by the dsrna mimic, pipc, but not from other apoptotic stimuli, fasl or staurosporine. csfv infection inhibits pipc-induced caspase activation, mitochondrial membrane potential loss and cytochrome c release as well as the pro-apoptotic effects of truncated bid (tbid) overexpression. the csfv proteins n(pr ... | 2010 | 20007358 |
| detection of african swine fever virus by loop-mediated isothermal amplification. | a loop-mediated isothermal amplification (lamp) assay was developed for the detection of african swine fever virus (asfv). this assay targets the topoisomerase ii gene of asfv and its specificity was confirmed by restriction enzyme digestion of the reaction products. the analytical sensitivity of this asfv lamp assay was at least 330 genome copies, and the test was able to detect representative isolates of asfv (n=38) without cross-reacting with classical swine fever virus. the performance of th ... | 2010 | 19963011 |
| the use of cos-1 cells for studies of field and laboratory african swine fever virus samples. | different naturally occurring, cell adapted or genetically manipulated stocks of african swine fever virus were able to infect directly cultures of cos-1 cells, producing extensive cytopathic effects and amounts from 10(6) to 10(7) of infective progeny virus per ml. the induction of late virus-specific proteins, demonstrated by rt-pcr and immunoblotting, and the development of lysis plaques by all the virus samples tested so far, allowed the optimization of both titration and diagnostic assays, ... | 2010 | 19961878 |
| classical swine fever virus ns3 enhances rna-dependent rna polymerase activity by binding to ns5b. | ns3 of pestiviruses contains a protease domain and a rna helicase/ntpase domain. contradictory results have been reported regarding ns3 in rna synthesis. to investigate the effect of ns3 on classical swine fever virus (csfv) ns5b rna-dependent rna polymerase activity (rdrp) activity and ns3-ns5b interaction, rdrp reactions, gst-pull-down assays and co-immunoprecipitation analyses containing ns5b and either of ns3 protein and the different truncated ns3 mutants were performed, respectively. we fo ... | 2010 | 19951725 |
| a host species-informative internal control for molecular assessment of african swine fever virus infection rates in the african sylvatic cycle ornithodoros vector. | african swine fever virus (asfv) infection in adult ornithodoros porcinus (murry 1877, sensuwalton 1979) ticks collected from warthog burrows in southern and east africa was assessed using a duplex genomic amplification approach that is informative with respect to the invertebrate host species and infecting sylvatic cycle virus. dna extracted from individual ticks was used as template for the simultaneous amplification of a c-terminal 478-bp asfv p72 gene region and a approximately 313-bp fragme ... | 2009 | 19941606 |
| antigens containing tavspttlr tandem repeats could be used in assaying antibodies to classical swine fever virus. | classical swine fever virus (csfv) causes a highly contagious and often fatal viral disease in pigs. the highly conserved epitope tavspttlr on the glycoprotein e2 was shown to be suitable for differentiation of csfv from other pestiviruses. in this study, we found that swine csfv antisera contained tavspttlr-related rather than tavspttlr-specific csfv antibodies. the csfv antisera reacted only to some extent with a synthetic tavspttlr-containing peptide, but inhibited the binding of tavspttlr-sp ... | 2009 | 19941387 |
| dynamin- and clathrin-dependent endocytosis in african swine fever virus entry. | african swine fever virus (asfv) is a large dna virus that enters host cells after receptor-mediated endocytosis and depends on acidic cellular compartments for productive infection. the exact cellular mechanism, however, is largely unknown. in order to dissect asfv entry, we have analyzed the major endocytic routes using specific inhibitors and dominant negative mutants and analyzed the consequences for asfv entry into host cells. our results indicate that asfv entry into host cells takes place ... | 2010 | 19939916 |
| [high expression of antimicrobial peptide cecropin ad in escherichia coli by fusion with eddie]. | in this study, we efficiently expressed the active antimicrobial peptide (cad), which fused with the site-mutated coat protein (eddie) of the classical swine fever virus, in escherichia coli. first, we obtained the e-cad fusion gene from the cad gene and the eddie gene using overlapping pcr. then to get the recombinant expression vector (peted), the e-cad fusion gene was cloned into the pet30a vector by a site-directed homologous recombination technique. the eddie-cad fusion protein expressed in ... | 2009 | 19938464 |
| development and evaluation of rapid detection of classical swine fever virus by reverse transcription loop-mediated isothermal amplification (rt-lamp). | a one-step reverse transcription loop-mediated isothermal amplification (rt-lamp) was developed for the detection of classical swine fever virus (csfv) and a set of primers designed based on the e2 gene reference sequences of the csfv. the assay was optimized to amplify csfv rna by incubation at 63 degrees c for 50 min. the rt-lamp amplification products had a ladder-like appearance when electrophoresed on an agarose gel. the rt-lamp assay showed higher sensitivity than the conventional rt-pcr, ... | 2010 | 19931578 |
| mutations in classical swine fever virus ns4b affect virulence in swine. | ns4b is one of the nonstructural proteins of classical swine fever virus (csfv), the etiological agent of a severe, highly lethal disease of swine. protein domain analysis of the predicted amino acid sequence of the ns4b protein of highly pathogenic csfv strain brescia (bicv) identified a putative toll/interleukin-1 receptor (tir)-like domain. this tir-like motif harbors two conserved domains, box 1 and box 2, also observed in other members of the tir superfamily, including toll-like receptors ( ... | 2010 | 19923180 |
| development and validation of reverse transcription loop-mediated isothermal amplification for detection of prrsv. | to establish a reverse-transcription loop-mediated isothermal amplification (rt-lamp) method for rapid detection of porcine reproductive and respiratory syndrome virus (prrsv), four primers specific to six regions of the n gene were designed. after amplification in an isothermal water bath for 1 h, samples containing prrsv generated the expected ladder-like products while porcine parvovirus, porcine circovirus, classic swine fever virus, pseudorabies virus, and swine testis cells generated no pr ... | 2010 | 19911264 |
| the classical swine fever virus npro product is degraded by cellular proteasomes in a manner that does not require interaction with interferon regulatory factor 3. | classical swine fever is a notifiable disease of pigs. the causative agent, classical swine fever virus (csfv), is highly contagious and causes mild to severe haemorrhagic disease depending on the virulence of the strain. the rna genome of csfv is translated as a single polyprotein that is processed to yield 12 proteins. like other pestiviruses, the first protein to be translated is the n-terminal autoprotease termed n(pro). a novel pestiviral protein with no known cellular homologues, n(pro) an ... | 2010 | 19906943 |
| interferon-gamma induction correlates with protection by dna vaccine expressing e2 glycoprotein against classical swine fever virus infection in domestic pigs. | classical swine fever (csf) is a highly contagious viral infection affecting domestic and wild pigs. for classical swine fever virus (csfv), immunization with plasmids expressing different versions of glycoprotein e2 has proven an effective way to induce protection. previously, we have also shown that immunization with dna vaccine expressing glycoprotein e2 (dna-e2) induced specific t helper cell responses in the absence of neutralizing antibodies. however, the role of t cell responses in protec ... | 2010 | 19896784 |
| phylogenetic analysis of recent classical swine fever virus (csfv) isolates from assam, india. | classical swine fever (csf), a highly contagious viral disease of pigs, is endemic in india. as there is no information concerning the accurate genetic typing of classical swine fever virus (csfv) isolates in india, 16 csf viruses isolated during 2005-2007 from domestic pigs in different districts of assam were typed in 5' utr (150 nucleotides). to confirm the genetic typing results and to study the genetic variability, selected viruses were also analyzed in e2 (190 nt) and ns5b gene (409 nt) re ... | 2011 | 19896713 |
| characterization of a new chimeric marker vaccine candidate with a mutated antigenic e2-epitope. | a new chimeric pestivirus "cp7_e1e2alf_tla", based on the infectious cdna of bovine viral diarrhea virus (bvdv) strain cp7, was constructed. the substitution of bvdv e1 and e2 with the respective proteins of classical swine fever (csf) strain alfort 187 allows an optimal heterodimerization of e1 and e2 in the chimeric virus, which is beneficial for efficient and authentic virus assembly and growth. in addition, for implementation of e2-based marker diagnostics, the previously described antigenic ... | 2010 | 19892497 |
| bovine viral diarrhea virus infections in heterologous species. | infections with bovine viral diarrhea virus (bvdv) are not limited to cattle, but may be detected in various species in the mammalian order artiodactyla. despite epidemiological evidence of bvdv infections in species other than cattle, current knowledge regarding the impact of bvdv on heterologous species is incomplete. in heterologous hosts, bvdv infections with clinical signs analogous to those in cattle have been described and include disease of multiple organ systems, most notably the reprod ... | 2010 | 19883524 |
| characterisation of experimental infections of domestic pigs with genotype 2.1 and 3.3 isolates of classical swine fever virus. | the early identification of classical swine fever epizootics is hampered by difficulties in recognising early signs of infection, due to a lack of specific clinical signs. in addition many textbook descriptions of csf are based on observations of disease caused by historic, mainly genotype 1, strains. our objective was to improve our knowledge of the diverse range of signs that different csfv strains can cause by characterising the experimental infection of domestic pigs with both a recent strai ... | 2010 | 19875252 |
| cultivation of the hog cholera virus. | the work of hecke on the cultivation, of hog cholera virus was confirmed with ease. virus was grown in the presence of fresh minced swine testicle in flasks containing tyrode solution, on the chorioallantoic membrane of embryonated eggs, and on the surface of swine serum agar. in flasks it was grown for 14 transfers; while on eggs it was grown for 13 transfers, followed by an equal number of transfers on agar, making 26 transfers in all. only one strain of virus was used and we do not know wheth ... | 1941 | 19871145 |
| the swine lungworm as a reservoir and intermediate host for swine influenza virus : ii. the transmission of swine influenza virus by the swine lungworm. | 1. the swine lungworm can serve as intermediate host in transmitting swine influenza virus to swine. the virus is present in a masked non-infective form in the lungworm, however, and, to induce infection, must be rendered active by the application of a provocative stimulus to the swine it infests. multiple intramuscular injections of h. influenzae suis furnish a means of provoking infection. swine influenza infections can be provoked in properly prepared swine during the autumn, winter, and spri ... | 1941 | 19871115 |
| histological studies on hog cholera : i. lesions in the central nervous system. | 1. a more or less marked encephalomyelitis and meningitis was found in 33 out of 39 cases of virus hog cholera which had been infected either intramuscularly or by contact and killed between 6 and 49 days after infection. 2. this hog cholera encephalitis is characterized by a varying amount of vascular and perivascular infiltration with small lymphocytes, mononuclear elements, a few plasma cells, and occasionally a few eosinophilic leucocytes. the glia shows a proliferation surrounding infiltrat ... | 1931 | 19869840 |
| variations in the plasma cholesterol and cholesterol ester content in hog cholera. | 1. the plasma cholesterol and cholesterol ester content of swine, experimentally infected with hog cholera, exhibit a regular succession of changes. during the period of incubation of the disease, for 3 or more days following inoculation with hog cholera virus, hypocholesterolemia prevails. this is followed by a period of hypercholesterolemia which is coincident with the onset of the clinical manifestations of the disease. the hypercholesterolemia after persisting for from 4 to 7 days, gives way ... | 1930 | 19869682 |
| bacilli of the hog-cholera group (bacillus cholerae suis) in man. | 1. the organisms isolated by hirschfeld from febrile cases resembling paratyphoid fever and named paratyphoid c can be placed in the hog-cholera bacillus group by their agglutination absorption properties though they are not typical culturally. 2. when fed to a pig a febrile disease resulted from which the animal recovered. after injection of hog-cholera virus the organisms fed were found generally, distributed and some of them had lost cultural characters so that they are brought into the class ... | 1920 | 19868429 |
| the survival of the hog-cholera virus in laboratory animals, particularly the rat. | the attempts to demonstrate the virus of hog-cholera in rabbits 12 days after intravenous and intraabdominal inoculations were unsuccessful. likewise the attempts to show that the virus might be found in the guinea pig 6 and in the pigeon 7 days after inoculation were negative. it was shown, however, that the virus can be found in the bodies of white rats for at least 7 days after either intraabdominal or intracerebral inoculations. one attempt to demonstrate it after 10 days was negative. from ... | 1918 | 19868292 |
| remarkable sequence similarity between the dinoflagellate-infecting marine girus and the terrestrial pathogen african swine fever virus. | heterocapsa circularisquama dna virus (hcdnav; previously designated as hcv) is a giant virus (girus) with a approximately 356-kbp double-stranded dna (dsdna) genome. hcdnav lytically infects the bivalve-killing marine dinoflagellate h. circularisquama, and currently represents the sole dna virus isolated from dinoflagellates, one of the most abundant protists in marine ecosystems. its morphological features, genome type, and host range previously suggested that hcdnav might be a member of the f ... | 2009 | 19860921 |
| classical swine fever: comparison of oronasal immunisation with cp7e2alf marker and c-strain vaccines in domestic pigs. | effective oronasal vaccination against classical swine fever (csf) is essential to achieve protection in wild boar. however the currently available live csf vaccines, e.g. c-strain, do not allow serological differentiation between infected and vaccinated animals (diva). a modified live marker vaccine candidate (cp7e2alf) has been recently developed (reimann et al., 2004). this communication reports the comparison of cp7e2alf and c-strain virus vaccines during 98 days following oronasal immunisat ... | 2010 | 19857935 |
| in vitro inhibition of the replication of classical swine fever virus by capsid-targeted virus inactivation. | classical swine fever virus (csfv) is the causative agent of classical swine fever (csf), a highly contagious fatal disease of swine. few effective antiviral drugs are currently available against csfv infections. to explore the feasibility of using capsid-targeted viral inactivation (ctvi) as an antiviral strategy against csfv infections, we expressed the csfv capsid protein (cap) fused with the nuclease of staphylococcus aureus (sn) in escherichia coli and investigated its effects on the replic ... | 2010 | 19857524 |
| a novel combination of taqman rt-pcr and a suspension microarray assay for the detection and species identification of pestiviruses. | the genus pestivirus contains four recognized species: classical swine fever virus, border disease virus, bovine viral diarrhoea virus types 1 and 2. all are economically important and globally distributed but classical swine fever is the most serious, concerning losses and control measures. it affects both domestic pigs and wild boars. outbreaks of this disease in domestic pigs call for the most serious measures of disease control, including a stamping out policy in europe. since all the member ... | 2010 | 19854591 |
| preventive vaccination contributes to control classical swine fever in wild boar (sus scrofa sp.). | over the last 20 years, oral vaccination implementing a live attenuated vaccine has been experimented in europe in order to control classical swine fever (csf) in wild boar (sus scrofa sp.). this has generally led to an enhanced seroprevalence and a decreased viroprevalence at the scale of the whole vaccinated populations, but no quantitative analysis has demonstrated the protective effect of preventive vaccination or intensive baiting. in the present paper we conducted a retrospective analysis ... | 2010 | 19854007 |
| characterisation of virus-specific peripheral blood cell cytokine responses following vaccination or infection with classical swine fever viruses. | existing live attenuated classical swine fever virus (csfv) vaccines provide a rapid onset of complete protection but pose problems in discriminating infected amongst vaccinated animals. with a view to providing additional information on the cellular mechanisms that may contribute to protection, which in turn may aid the development of the next generation of csfv vaccines, we explored the kinetics of the cytokine responses from peripheral blood cells of pigs vaccinated with an attenuated c-strai ... | 2010 | 19854006 |
| the effect of tissue degradation on detection of infectious virus and viral rna to diagnose classical swine fever virus. | a considerable part of tissue samples that are collected for the monitoring of classical swine fever (csf) from the wild boar population or from domestic pigs are unsuitable for virus detection using the fluorescent antibody test (fat) or virus isolation (vi), due to tissue degradation. reverse transcription polymerase chain reaction (rt-pcr) has a higher sensitivity than the fat and vi, and is supposed to be less sensitive to sample degradation. reliable and quantitative information on how long ... | 2010 | 19854005 |
| a socio-psychological investigation into limitations and incentives concerning reporting a clinically suspect situation aimed at improving early detection of classical swine fever outbreaks. | the aim of this study was to identify limitations and incentives in reporting clinically suspect situations, possibly caused by classical swine fever (csf), to veterinary authorities with the ultimate aim to facilitate early detection of csf outbreaks. focus group sessions were held with policy makers from the veterinary authorities, and representatives of veterinary practitioners and pig farmer unions. personal interviews with a small group of pig farmers and practitioners were held to check li ... | 2010 | 19854004 |
| rna interference targeting nucleocapsid protein (c) inhibits classical swine fever virus replication in sk-6 cells. | the application of rna interference (rnai) strategy for controlling classical swine fever could become a promising alternative to the conventional eradication measures, as it was recently shown for foot-and-mouth disease (chen et al., 2004), influenza (ge et al., 2003), porcine reproductive and respiratory syndrome (he et al., 2007) and porcine transmissible gastroenteritis (zhou et al., 2007). the use of synthetic sirna which is corresponding to nucleotides 1130-1148 of the csf virus strain alf ... | 2010 | 19850420 |
| african swine fever virus polyprotein pp62 is essential for viral core development. | one of the most characteristic features of african swine fever virus gene expression is its use of two polyproteins, pp220 and pp62, to produce several structural proteins that account for approximately 32% of the total protein virion mass. equimolecular amounts of these proteins are the major components of the core shell, a thick protein layer that lies beneath the inner envelope, surrounding the viral nucleoid. polyprotein pp220, which is located immediately underneath the internal envelope, i ... | 2010 | 19846532 |
| changes of immunophysiological characteristics in neonatal calves experimentally challenged with mixture of live bacteria and virus. | the aim of the present study was to define efficient immunophysiological parameters in neonatal holstein calves with an experimentally induced microbial infection. calves (n = 15) were challenged with classical swine fever virus (lom strain) and erysipelothrix insidiosa live vaccine by intravenous injection at 3 wk of age except for control calves (n = 4). the level of total serum iga was significantly increased at 14 and 19 d post-experimental challenge (dpec) compared with that in calves at -2 ... | 2009 | 19841216 |
| generation of recombinant pestiviruses using a full-genome amplification strategy. | complete genome amplification of viral rna provides a new tool for the generation of modified viruses. we have recently reported a full-genome amplification strategy for recovery of pestiviruses (rasmussen et al., 2008). a full-length cdna amplicon corresponding to the border disease virus-gifhorn genome was generated by long rt-pcr and then rna transcripts derived from this amplicon were used to rescue infectious virus. here, we have now used this full-genome amplification strategy for efficien ... | 2010 | 19836906 |
| genetic typing of recent classical swine fever isolates from india. | seventeen classical swine fever virus (csfv) isolates recovered during the period of 3 years (2006-2008) from india were subjected to nucleotide sequencing in the 5' untranslated region (utr). for genetic typing, 150 nucleotides within this region were used. for better epizootiological understanding, 39 nucleotide sequences of the above region, including 13 indian csfv sequences, available either in the genbank or published literature were also included in the study. based on the phylogenetic an ... | 2010 | 19836905 |
| establishment of an in vitro system for the prediction of the degree of virulence of classical swine fever virus isolates. | 2007 | 19835079 |