Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| putative spawner-isolated mortality virus associated with mid-crop mortality syndrome in farmed penaeus monodon from northern australia. | beginning in 1994, farms in northern australia experienced a higher than normal mortality rate in 12 to 15 g prawns from growout ponds. the farmers named this problem mid-crop mortality syndrome (mcms). intramuscular injection of filtered (450 nm), cell-free extracts of moribund prawns from these ponds killed healthy prawns between 5 to 30 d post-injection. a 20 nm virus was visualized by electron microscopy from a 1.4 g ml-1 band recovered from caesium chloride gradients of extracts from the mo ... | 1998 | 9891733 |
| dietary biotin requirement for maximum growth of juvenile grass shrimp, penaeus monodon. | a feeding trial was conducted to estimate the minimal dietary biotin requirement for juvenile grass shrimp, penaeus monodon. purified diets with eight levels (0, 0.2, 0.5, 1.0, 3.0, 6.0, 10.0 and 20.0 mg/kg) of supplemental biotin were fed to p. monodon (mean weight 0. 26 +/- 0.01 g) for 8 wk. each diet was fed to three replicate groups of shrimp. shrimp fed diets supplemented with biotin (0.2-20.0 mg/kg) had significantly (p < 0.05) higher weight gain, feed efficiency and protein efficiency rat ... | 1998 | 9868198 |
| genetic structure in wild populations of black tiger shrimp (penaeus monodon) using randomly amplified polymorphic dna analysis. | randomly amplified polymorphic dna (rapd) analysis was used to examine genetic variation in wild black tiger shrimp, penaeus monodon. specimens were collected from five geographically separated locations (satun-trang, phangnga, and medan in the andaman sea and chumphon and trad in the gulf of thailand). a total of 100 p. monodon individuals were investigated using seven arbitrarily selected primers. fifty-eight (72.5%) of eighty reproducible rapd fragments ranging in size from 200 to 2200 bp wer ... | 1998 | 9852621 |
| histopathology of cultured shrimp showing gross signs of yellow head syndrome and white spot syndrome during 1994 indian epizootics. | two epizootics of cultured shrimp occurred in india in 1994. the first case of mass mortalities of tiger shrimp penaeus monodon in july 1994 was very similar to that caused by yellowhead virus (yhv) infection with regard to gross clinical signs, host species and size of shrimp affected, but it was histologically atypical. very interestingly, intranuclear inclusions typical of a white spot syndrome virus (wssv) infection were present in shrimp showing gross signs of yellow head syndrome, suggesti ... | 1998 | 9789974 |
| experimental transmission of white spot syndrome virus (wssv) from crabs to shrimp penaeus monodon. | white spot syndrome virus (wssv) of the black tiger prawn penaeus monodon is a recently discovered baculo-like virus disease which is currently the cause of very serious and widespread losses in the shrimp industry in thailand and elsewhere in asia. three suspected crab carriers of this virus commonly found in shrimp-rearing areas were investigated. these were sesarma sp., scylla serrata and uca pugilator. all these crabs could be infected with wssv by injection and they sustained heavy viral in ... | 1998 | 9789973 |
| colourimetric pcr-based detection of monodon baculovirus in whole penaeus monodon postlarvae. | the development of a nested polymerase chain reaction (pcr) assay is described to detect low concentrations of monodon baculovirus (mbv) dna from total penaeus monodon postlarval dna. a modified dna extraction procedure was also developed to circumvent problems associated with co-purification of pcr inhibitors in total dna extracted from whole postlarvae. this method involved mechanical disruption of frozen prawn material immediately followed by phenol extraction at high temperature. an assessme ... | 1998 | 9763125 |
| diurnal variation and effects of feeding on blood glucose in the giant tiger prawn, penaeus monodon. | previous studies on crustacea have demonstrated significant diurnal rhythms in blood glucose. however, glucose concentration in the blood of food-deprived penaeus monodon, held in indoor or outdoor tanks, did not exhibit a diurnal rhythm under photoperiods of 8 h light and 16 h darkness (8l: 16d) or under a 13.5l: 9.5d photoperiod, with simulated or natural full moon conditions. prawns held on photoperiods of constant light, 20l : 4d, 16l : 8d, 12l : 12d, 8l : 16d, 4l : 20d, or continuous darkne ... | 1998 | 9754534 |
| a preliminary study of ribosomal dna polymorphism in the tiger shrimp, penaeus monodon. | the preliminary study of rdna polymorphisms in p. monodon showed that inter- and intraindividual polymorphisms of rdna were clearly observed in this species. individual-specific rdna restriction patterns were observed when digested with bamhi and saci. the intergenic spacers (igs) region of p. monodon rdna plays an important role in length heteroplasmy at both between- and within-individual levels in this species. | 1998 | 9701643 |
| chitin but not chitosan supplementation enhances growth of grass shrimp, penaeus monodon. | the effect of chitin, poly-beta-(1 --> 4)-n-acetyl-glucosamine, and chitosan, a polymer of glucosamine obtained by the deacetylation of chitin, on growth and nutrient digestibility was studied in grass shrimp, penaeus monodon. shrimp were fed for 8 wk diets containing no supplement (control) or 2, 5 or 10 g/100 g chitin or chitosan. each diet was fed to triplicate groups of shrimp with a mean initial body weight of 0.45 +/- 0.05 g. significantly higher body weight gains were observed in shrimp f ... | 1998 | 9567002 |
| multidrug- and metal-resistant strains of klebsiella pneumoniae isolated from penaeus monodon of the coastal waters of deltaic sundarban. | marine shrimp of the species penaeus monodon were collected from the coastal region (haroa) of the deltaic sundarbans of west bengal, india during the premonsoon period in 1996. klebsiella pneumoniae was isolated from the alimentary canal and gills of the shrimp as the sole isolate. all 10 isolated strains were resistant to erythromycin (30 micrograms/ml), ampicillin (100 micrograms/ml), furazolidone (100 micrograms/ml), and penicillin (100 iu). these strains were able to grow in the presence of ... | 1998 | 9543720 |
| phenoloxidase activity of hemocytes derived from penaeus monodon and macrobrachium rosenbergii. | the phenoloxidase (po) activity of hemocyte lysate supernatant (hls) from both tiger shrimp (penaeus monodon) and giant freshwater prawn (macro-branchium rosenbergii) was examined by treating hls with various factors, such as an increase in temperatures from 25 to 70 degrees c, one of four elicitors (beta-1,3-1,6-glucan, zymosan, heat-killed vibrio cells, and lipopolysaccharide), trypsin, one of three protease inhibitors (soybean trypsin inhibitor, p-nitrophenyl-p'-guanidinobenzoate, and benzami ... | 1998 | 9446734 |
| effects of extracellular products of vibrio alginolyticus on penaeid prawn plasma components. | the effects of both crude extracellular products (ecp) and a partially purified protease of vibrio alginolyticus on the plasma components of kuruma prawn (penaeus japonicus) and tiger prawn (p. monodon) were studied using crossed immunoelectrophoresis (cie). a component of the plasma, tentatively identified as coagulogen, apparently disappeared after incubation with the ecp, while the amount of a component tentatively identified as haemocyanin decreased. the coagulogen and an unknown component ( ... | 1997 | 9281857 |
| glucan-induced disease resistance in tiger shrimp (penaeus monodon). | non-specific disease resistance induced by yeast cell wall extract, beta-1,3-1,6-glucan, was demonstrated in the tiger shrimp. in this study beta-1,3-1,6-glucan was administered to shrimps by immersion before culturing and orally during the culturing period. challenge of the treated shrimps with the virulent pathogens, vibrio vulnificus and viral agents extracted from the white spot syndrome victims, yielded promising results. the tolerance of glucan-treated shrimps was slightly enhanced to stre ... | 1997 | 9270870 |
| passive immunization of the tiger prawn, penaeus monodon, using rabbit antisera to vibrio harveyi. | passive immunization, toxicity neutralization and the persistence of passive protection in the tiger prawn (penaeus monodon) were investigated using rabbit antisera to the formalinized extracellular products (ecp) (r alpha ecp) and/or formalinized bacterial cells (r alpha bc) of luminescent vibrio harveyi strain 820514 originally isolated from diseased tiger prawns. rabbit antiserum to bovine serum albumin (r alpha bsa) or phosphate-buffered saline (pbs, ph 7.2) both served as controls. the toxi ... | 1997 | 9248078 |
| random amplified polymorphic dna (rapd) markers for determination of genetic variation in wild populations of the black tiger prawn (penaeus monodon) in thailand. | random amplified polymorphic dna (rapd) analysis was used to amplify the genome of black tiger prawns (penaeus monodon) to detect dna markers and assess the utility of the rapd method for investigating genetic variation in wild p. monodon in thailand. a total of 200 ten-base primers were screened, and 84 primers yielded amplification products. six positive primers that gave highly reproducible rapd patterns were selected for the analysis of three geographically different samples of thai p. monod ... | 1997 | 9200837 |
| purification and characterization of a cysteine protease produced by pathogenic luminous vibrio harveyi. | the purification and characterization of an extracellular protease produced by pathogenic luminous vibrio harveyi strain 820514, originally isolated from diseased tiger prawn (penaeus monodon), was presented in this paper. the purification steps included ammonium sulfate precipitation, with columns of hydrophobic interaction chromatography and anion exchange on fast protein liquid chromatography. the protease is an alkaline cysteine protease, heat labile, inhibited by iodoacetamide, iodoacetic a ... | 1997 | 9175557 |
| toxicity of cyanide, iron-cyanide complexes, and a blast-furnace effluent to the banana prawn, penaeus monodon. | 1997 | 9115149 | |
| development of a non-radioactive gene probe by pcr for detection of white spot syndrome virus (wssv). | combining primers created from the sequence information of two baculo-like viruses of penaeid shrimp, baculovirus penaei (bp) and monodon baculovirus (mbv), produced a 750 bp band on a 0.8% agarose gel using white spot syndrome virus (wssv), from penaeus monodon, as the dna template. the pcr fragment was ligated to a plasmid vector, (pgem-t) and transformed, creating a 3.7 kbp clone. the dna insert was sequenced, and the original primer pair was located. using restriction enzymes, the insert was ... | 1997 | 9015290 |
| isolation and molecular identification of planctomycete bacteria from postlarvae of the giant tiger prawn, penaeus monodon. | bacteria phenotypically resembling members of the phylogenetically distinct planctomycete group of the domain bacteria were isolated from postlarvae of the giant tiger prawn, penaeus monodon. a selective medium designed in the light of planctomycete antibiotic resistance characteristics was used for this isolation. planctomycetes were isolated from both healthy and monodon baculovirus-infected prawn postlarvae. the predominant colony type recovered from postlarvae regardless of viral infection s ... | 1997 | 8979353 |
| isolation and characterization of vibrio alginolyticus isolated from diseased kuruma prawn, penaeus japonicus. | outbreaks of serious mortality among cultured kuruma prawns (penaeus japonicus) with white spotted syndrome in the carapace occurred in the summer of 1993 in i-lan, taiwan. a swarming bacterium, strain swy, was isolated from the hepatopancreas of the moribund prawns using tryptic soy agar supplemented with 1% nacl and/or thiosulphate citrate bile salt sucrose agar. this strain was characterized and identified as vibrio alginolyticus on the basis of a number of biochemical tests. the swy strain w ... | 1996 | 8936369 |
| toxic effects of blooms of marine species of oscillatoriales on farmed prawns (penaeus monodon, penaeus japonicus) and brine shrimp (artemia salina). | benthic and planktonic blooms of species of oscillatoriales coincided with mortalities of penaeus monodon during four episodes at australian prawn farms. oscillatoria corakiana was the dominant planktonic species at 65-90,000 cells/ml, but spirulina sp., lyngbya sp., oscillatoria sp. and nodularia sp. were also identified from the water column, benthic layers or surface mats. the levels and variety of vibrionaceae in prawn tissue, suggest that mortalities were caused by secondary infections of b ... | 1996 | 8875773 |
| virulence of vibrio alginolyticus isolated from diseased tiger prawn, penaeus monodon. | outbreaks of mass mortality among cultured tiger prawns (penaeus monodon) with white spotted syndrome (wss) in the carapace occurred in the summer of 1994 in i-lan, taiwan. a swarming strain val was isolated from hemolymph of the moribund prawns with tryptic soy agar (tsa, supplemented with 1% nacl, oxoid) and/or thiosulfate citrate bile salt sucrose (tcbs, difco) agar. this strain was characterized and identified to be vibrio alginolyticus. the strain was susceptible to antibiotics such as chlo ... | 1996 | 8867464 |
| isolation of vibrio harveyi from diseased kuruma prawns penaeus japonicus. | outbreaks of high mortality among the cultured kuruma prawn penaeus japonicus without overt gross signs occurred during august and december of 1994 in i-lan, taiwan. eleven luminous bacterial strains were isolated from the hepatopancreas of moribund prawns from five different farms by use of tryptic soy agar (tsa, supplemented with 2% nacl) and/or thiosulfate citrate bile salt sucrose (tcbs) agar. these strains, together with our two previously unpublished isolates, were characterized and identi ... | 1996 | 8662185 |
| [preliminary studies of the immunization of shrimp (penaeus monodon) against vibrio infections]. | there is a great demand for an applicable vaccine against bacterial infections of prawns, especially vibriosis. the results of the tests that had been carried out can be evaluated as promising and indicate that the vaccination of prawns against bacterial diseases is possible. nevertheless it is still necessary to increase the scale of research on this subject, above all, the basics of the immuno-system of prawns. adult prawns should be vaccinated to check if they are able to pass their immuno-pr ... | 1995 | 8651898 |
| n-acetyltransferase, hydroxyindole-o-methyltransferase and melatonin in the optic lobes of the giant tiger shrimp penaeus monodon. | the activities of the enzymes n-acetyltransferase (nat) and hydroxyindole-o-methyltransferase (hiomt) and the hormone melatonin were studied in the optic lobe of the subadult giant tiger shrimp penaeus monodon. compared with the level in other species, a relatively high level of nat activity that was temperature- and ph-dependent were observed. the nat enzyme had a relatively high maximum velocity (vmax, 100 pmol/hr/micrograms protein) and low michaelis constant (km, 22 microm), when tryptamine ... | 1995 | 8531052 |
| specific phosphorylation of membrane proteins of mr 44,000 and mr 32,000 by the autophosphorylated insulin receptor from the hepatopancreas of the shrimp penaeus monodon (crustacea: decapoda). | the insulin receptor, purified from the hepatopancreas of the shrimp penaeus monodon, is a hydrophobic heterodimer of subunits with relative masses (mr) of 70,000 and 58,000, as estimated by fplc on superose 12 and sds-page. only the subunit of mr 70,000 was autophosphorylated after the addition of insulin. the autophosphorylation occurred specifically at tyr residues, as demonstrated by the specific subsequent dephosphorylation by the phosphotyrosyl protein phosphatase from the hepatopancreas o ... | 1993 | 8409897 |
| quantifying the vitamin k requirement of juvenile marine shrimp (penaeus monodon) with menadione. | a feeding trial was conducted to estimate the optimum dietary vitamin k requirement for juvenile marine shrimp, penaeus monodon. purified diets with eight levels (0, 5, 10, 20, 50, 80, 160 and 320 mg/kg) of supplemental menadione were fed to p. monodon (mean weight 1.33 +/- 0.05 g) for 12 wk. each diet was fed to three replicate groups of shrimp. shrimp fed diets supplemented with vitamin k (5-320 mg/kg) had significantly (p < 0.05) higher weight gain and feed efficiency than those fed the unsup ... | 1994 | 8308577 |
| purification and amplification of dna from penaeus monodon-type baculovirus (mbv). | the purpose of this paper was to purify and amplify the dna fragment of penaeus monodon-type baculovirus (mbv). using 30-50% caesium chloride gradients, mbv virions and occlusion bodies with density parameters of 1.28-1.29 and 1.32-1.33 g/ml, respectively, were purified. two oligonucleotide primers have been successfully designed and utilized for the amplification of a dna fragment of mbv. after 35 amplification cycles of the mbv dna fragment, a large amount of amplified product with an approxim ... | 1993 | 8228316 |
| toxicity of nickel ores to marine organisms. | queensland nickel proposes to import new caledonian (ballande) and indonesian (gebe) nickel ores, one option being ship-to-barge transfer in halifax bay, north queensland. because small amounts of ore may be split during the unloading and transfer operations, it was important to investigate the potential impact of the spilt ore on the ecological health of the bay. long-term leaching of the ores with seawater showed that only nickel and chromium (vi) were released from the ores in sufficient conc ... | 1994 | 8029690 |
| immunostimulation of tiger shrimp (penaeus monodon) hemocytes for generation of microbicidal substances: analysis of reactive oxygen species. | nbt staining was used to determine the presence of superoxide anions (o2-) produced by tiger shrimp (penaeus monodon) hemocytes attached to a coverslip. when cells were treated with beta-glucan, blue granules were observed in 41% of studied hemocyte cytoplasm. for zymosan-treated, pma-treated, and control cells, the percentages of hemocytes showing similar blue granules were 31, 9, and 5%, respectively. a comparison of stimulative effects on 15 hemocyte suspensions, each collected from a single ... | 1994 | 8001699 |
| quantification of vitamin c requirements for juvenile shrimp (penaeus monodon) using polyphosphorylated l-ascorbic acid. | the vitamin c requirements of marine shrimp (penaeus monodon) for optimal growth were evaluated in a 15-wk feeding trial using polyphosphorylated l-ascorbic acid (c2pp), a stable derivative of l-ascorbic acid (c1). juvenile shrimp (0.55 +/- 0.01 g) were fed purified diets containing graded levels (0, 50, 100, 200 and 400 mg/kg diet) of supplemental c2pp or a high dosage of c1 (2500 mg/kg diet). their weight gain, survival, feed efficiency and c1 storage in hepatopancreas and muscle were used to ... | 1994 | 7931713 |
| penaeid prawns and associated luminous bacteria. | luminous bacteria associated with the exoskeleton, gill and gut of penaeid prawns, penaeus indicus h. milne edwards and penaeus monodon fabricius in mangalore waters were isolated, identified and quantified. two species of bacteria, vibrio harveyi and vibrio fischeri were recorded, the former was dominant in the exoskeleton and gill of both the penaeids. gut of prawns supported exclusive and dense populations of v. harveyi suggesting that the species is well adapted to proliferate in this microe ... | 1994 | 7866726 |
| use of non-radioactively labeled dna probes for the detection of a baculovirus from penaeus monodon by in situ hybridization on fixed tissue. | clones isolated from a genomic library of the baculovirus pmsnpv (= mbv) were used to prepare dna probes for detection of pmsnpv in shrimp tissue. davidson's afa fixed shrimp tissues were probed using dna that had been labeled with the hapten, digoxigenin, by an in situ hybridization assay. the dna probes readily distinguished pmsnpv-infected from uninfected shrimp tissue. the reactions were localized to the hepatopancreas and midgut, the sites where this virus is detected by standard histologic ... | 1994 | 7822460 |
| toxic effects of dissolved copper on penaeus merguiensis and penaeus monodon. | 1995 | 7663095 | |
| purification of the delta isoenzyme of protein kinase c from the hepatopancreas of the shrimp penaeus monodon with phosphorylation on tyrosine residues. | the delta isoenzyme of protein kinase c (pkc-delta), purified from the plasma membrane of the hepatopancreas of the shrimp penaeus monodon is specifically phosphorylated at tyrosine residues, as demonstrated by specific dephosphorylation by phosphotyrosyl protein phosphatase from the hepatopancreas of the shrimp penaeus monodon. the specific activity of purified pkc-delta was 200 units/mg of protein. the subunits of m(r) 66,000, 62,000, and 58,000 of pkc-delta were not autophosphorylated after t ... | 1995 | 7650514 |
| toxic effects of linear alkylbenzene sulfonate on the tiger prawn, penaeus monodon. | the toxic effects of linear alkybenzene sulfonate (las) on the larvae and juveniles of tiger prawn penaeus monodon were tested. the 24-hr lc50 values of las were 0.06, 0.10, and 3.11 ppm for the zoea 2nd substage, mysis 2nd substage (m2), and postlarva 12th substage (pl12) of the tiger prawn, respectively. the 48-hr lc50 values of las were 0.07, 1.03, and 4.36 ppm for m2, pl12, and postlarva 15th substage of the tiger prawn, respectively. the hepatopancreatic glutathione (gsh) in juvenile tiger ... | 1993 | 7507819 |
| cytomorphic studies of the thoracic ganglia of the prawn penaeus monodon (fabricius) (order: decapoda; suborder: macrura; family: penaeidae). | 1985 | 4054754 | |
| detection of a baculovirus in the tiger prawn penaeus monodon. | in postlarval penaeus monodon from a hatchery in asia a baculovirus was detected in the epithelial cells of the hepatopancreas by electron microscopy. the baculovirus was identified as the monodon baculovirus (mbv). | 1989 | 2763758 |
| effects of phosphamidon and lindane on the limb regeneration of penaeid prawn, penaeus monodon. | 1989 | 2466503 | |
| existence of a gonadotropin-releasing hormone-like factor in the grass shrimp (black tiger prawn, penaeus monodon) | the immunological and biological gonadotropin-releasing hormone (gnrh)-like activities in the acid extract of shrimp hepatopancreas (she) were studied. the immunological activity determined by a gnrh radioimmunoassay was 22.3 +/- 2.7 ng per ml of she. she increased luteinizing hormone (lh) release from rat anterior pituitary glands (aps) in vitro, and its biological activity was 42.9 +/- 28.5 ng per ml. no immunological activity was found in the acid extracts of eye ball, eye stalk, nerve and he ... | 1990 | 2257769 |
| a comparative study of alkaline phosphatases among human placenta, bovine milk, hepatopancreases of shrimp penaeus monodon (crustacea: decapoda) and clam meretrix lusoria (bivalvia: veneidae): to obtain an alkaline phosphatase with improved characteristics as a reporter. | 1. alkaline phosphatases were purified from human placenta, bovine milk, shrimp and clam with a final spec. act. of 67,000, 32,000, 22,000 and 15,000 u/mg of protein respectively. 2. the alkaline phosphatase from meretrix lusoria is unique with its thermostability at 65 degrees c for 30 min; whereas the remaining enzymes studied, including the human placental alkaline phosphatase, are inactivated and have negligible activities. 3. the alkaline phosphatase from penaeus monodon can be differentiat ... | 1990 | 2225775 |
| the presence of a thyrotropin-releasing hormone-like factor in the grass shrimp (black tiger prawn, penaeus monodon) | immunoreactive (trh) has been detected in acid and methanol extracts of hepatopancreas, in grass shrimp (black tiger prawn, penaeus monodon). the evidence provided by the stimulation of thyrotropin (tsh) release from perfused rat anterior pituitary glands in vitro following the application of hepatopancreas extracts (she) reflected the presence of trh-like substance in the shrimp. however, the molecular weight of the trh-like substance estimated by gel filtration in she was greater than that of ... | 1990 | 2124182 |
| the midgut trypsins of shrimp (penaeus monodon). high efficiency toward native protein substrates including collagens. | two shrimp trypsins have been purified from the midguts of penaeid shrimps by various chromatographies and hplc. the molecular masses of them are 27 and 29 kda, respectively. they show the typical specificity of trypsin for substrates and inhibitors, and their n-terminal amino-acid sequences are homologous to those of other trypsins. the shrimp enzymes are very acidic (pi less than or equal to 2.4), and show distinctively low km for the synthetic amide substrates. they also hydrolyse various nat ... | 1990 | 1963309 |
| thiamin requirement of juvenile shrimp (penaeus monodon). | in a 9-wk feeding trial, juvenile shrimp (penaeus monodon) were fed semipurified diets containing seven levels (0, 20, 40, 60, 80, 160 and 320 mg/kg diet) of supplemental thiamin hydrochloride. the dietary thiamin level required for optimal growth in p. monodon was found to be approximately 14 mg/kg diet based on hemolymph (blood) thiamin analysis. the minimum dietary thiamin level that produced substantial shrimp growth was approximately 13 mg/kg diet. shrimp fed unsupplemented diets (thiamin c ... | 1991 | 1941262 |
| the carotenoids of wild and blue disease affected farmed tiger shrimp (penaeus monodon, fabricus). | 1. the main carotenoids in wild penaeus monodon exoskeleton were astaxanthin di- and mono-esters, astaxanthin, and beta-carotene. 2. wild p. monodon exoskeleton contained on average 26.3 ppm total carotenoid; normally pigmented farmed shrimp had a similar concentration (25.3 ppm). 3. exoskeletons of farmed "blue" p. monodon (i.e. blue-coloured, as opposed to the normally red-blue/black banded shrimp) contained significantly less total carotenoid (4.3-7 ppm). the only major carotenoid being astax ... | 1991 | 1873990 |
| in vivo decondensation of chromatin and nucleolar fibrillar component by leucaena leucocephala ingredient. | feeding of the shrimp, penaeus monodon, with diets containing leaf meal of the leguminous shrub, leucaena leucocephala, resulted in complete chromatin decondensation of hepatopancreas cells. the fibrillar component of the nucleolus was decondensed in parallel, whereas the granular component remained intact. this unique combination of nuclear signs was accompanied by only moderate alterations of other cell organelles. our findings therefore demonstrate an encouraging possibility to manipulate the ... | 1991 | 1794061 |
| collagenolytic activity of crustacean midgut serine proteases: comparison with the bacterial and mammalian enzymes. | 1. we have investigated the collagenolytic activity of the following serine proteases: proteinase k, subtilisin novo, staphylococcal endoproteinase glu-c, streptomyces pronases, the trypsins and chymotrypsins from shrimp midgut and bovine pancreas. 2. by assays on both the insoluble 3h-collagen fibrils and the soluble type i collagen, it was demonstrated that the shrimp midgut serine proteases, and less efficiently, the pronases from streptomyces griseus, could hydrolyze collagen while the other ... | 1991 | 1782759 |
| isolation of a bacterium resembling pirellula species from primary tissue culture of the giant tiger prawn (penaeus monodon). | during attempts to establish tissue cultures from hepatopancreas, heart, and hemolymph of the giant tiger prawn (penaeus monodon), using a medium including penicillin, streptomycin, and amphotericin b, bacterial contamination in the form of a sheet of growth attached to the tissue culture vessel was a persistent problem. contaminant bacteria were teardrop-shaped cells arranged in rosettes, and electron microscopy revealed buds, crateriform structures, and the absence of a peptidoglycan layer in ... | 1991 | 1781677 |
| the basic isoelectric form of alpha-l-fucosidase from the hepatopancreas of the shrimp penaeus monodon (crustacea: decapoda). | 1. alpha-l-fucosidase was purified ca 10,889-fold to homogeneity from penaeus monodon, with a final spec. act. of 31,250 u/mg of protein. 2. by using sds-polyacrylamide gel electrophoresis, the monomers of shrimp alpha-l-fucosidase were discovered to have mol. wts of 63,000 and those of human placental enzyme, 46,000 and 20,000. since the active shrimp alpha-l-fucosidase was found to have a mol. wt of 233,000 by superose 12 fplc, it was concluded that the purified shrimp enzyme was tetrameric. 3 ... | 1991 | 1764918 |
| crustacean hyperglycemic hormone induced alterations in millepede (spirostreptus asthenes) carbohydrate metabolism. | following intersegmental injection of the hyperglycemic hormone (hgh) obtained from fresh water crab (oziotelphusa senex senex) and marine tiger prawn (penaeus monodon), hemolymph sugar concentration was significantly increased in millepede (spirostreptus asthenes). glycogen and total carbohydrates of fat body decreased in injected animals indicating that the source of hyperglycemia was carbohydrates from the fat body. correlating with the decreased glycogen content, phosphorylase activity of th ... | 1991 | 1725740 |
| the midgut chymotrypsins of shrimps (penaeus monodon, penaeus japonicus and penaeus penicillatus). | the midgut chymotrypsins (ec 3.4.4.5) of three species of shrimps, penaeus monodon, penaeus japonicus and penaeus penicillatus were purified and studied in detail to clarify previous ambiguity in their identification. in each of the species there are two major forms of chymotrypsin, both single-chained with three disulfide bonds. one has a pi of 3.2 and mr 27,000 or 28,000, while the other has a pi of 3.0 and mr 25,000 or 26,000. the n-terminal amino acid sequences of the p. monodon enzymes are ... | 1991 | 1657178 |
| estimation of the dietary riboflavin required to maximize tissue riboflavin concentration in juvenile shrimp (penaeus monodon). | the riboflavin requirements of marine shrimp (penaeus monodon) were evaluated in a 15-wk feeding trial. juvenile shrimp (initial mean weight, 0.13 +/- 0.05 g) were fed purified diets containing seven levels (0, 8, 12, 16, 20, 40 and 80 mg/kg diet) of supplemental riboflavin. there were no significant differences in weight gains, feed efficiency ratios and survival of shrimp over the dietary riboflavin range. the riboflavin concentrations in shrimp bodies increased with the increasing vitamin sup ... | 1992 | 1453232 |
| progesterone metabolism in vitro in the decapod crustacean, penaeus monodon. | thin-layer chromatography (tlc) and reversed-phase high-performance liquid chromatography (rp-hplc) with on-line detection of radioactive steroids were applied to identify metabolites of [4-14c]progesterone incubated in vitro with prawn ovary. there was extensive metabolism of progesterone by stage ii (vitellogenic) ovary of penaeus monodon. the most abundant metabolites were 5 alpha-pregnane derivatives together with two minor metabolites, 20 alpha-hydroxypregn-4-en-3-one and 1,4-pregnadiene-3, ... | 1992 | 1398023 |
| changes in carbohydrate metabolism of pila globosa in response to crustacean hyperglycemic hormone. | hyperglycemia was caused in the snail pila globosa by the injection of the hyperglycemic hormones obtained from fresh water crabs (oziotelphusa senex senex) and marine tiger prawns (penaeus monodon). tissue glycogen and total carbohydrates presented a significant decrease which indicated that the source of hyperglycemia was the tissue carbohydrates. the hyperglycemic principles also increased the tissue phosphorylase activity and provided evidence for a possible action of the crustacean hypergly ... | 1992 | 1382680 |