Publications

nematode pyruvate dehydrogenase kinases: role of the c-terminus in binding to the dihydrolipoyl transacetylase core of the pyruvate dehydrogenase complex.	pyruvate dehydrogenase kinases (pdks) from the anaerobic parasitic nematode ascaris suum and the free-living nematode caenorhabditis elegans were functionally expressed with hexahistidine tags at their n-termini and purified to apparent homogeneity. both recombinant pdks (rpdks) were dimers, were not autophosphorylated and exhibited similar specific activities with the a. suum pyruvate dehydrogenase (e1) as substrate. in addition, the activities of both pdks were activated by incubation with pdk ...	1999	10085233
molecular-weight-dependent effects of nonanticoagulant heparins on allergic airway responses.	we have hypothesized that antiallergic activity of inhaled heparin is molecular weight dependent and mediated by "nonanticoagulant fractions" (naf-heparin). therefore, we studied comparative effects of high-, medium-, and ultralow-molecular-weight (hmw, mmw, and ulmw, respectively) naf-heparins on acute bronchoconstrictor response (abr) and airway hyperresponsiveness (ahr) in allergic sheep. specific lung resistance was measured in 23 allergic sheep, before and immediately after challenge with a ...	1999	9931190
interactions between the nematode parasite of pigs, ascaris suum, and the earthworm aporrectodea longa.	pig faeces in which ascaris suum eggs had been embryonating for 57 days were placed in buckets of soil containing either 30 or no earth-worms (aporrectodea longa). when present, earthworms consumed the faeces and transported the eggs down into the soil, without inflicting any visible damage on the eggs. in later experiments 10 earthworms from the above experiment were fed to each of ten pigs, and another 40 earthworms were dissected. none of the 10 pigs became infected with a. suum through consu ...	1998	9926459
isolation, pharmacology and gene organization of kpsfvrfamide: a neuropeptide from caenorhabditis elegans.	to date, 53 peptides with c-terminal rfamides have been identified by the genome sequencing project in the nematode, caenorhabditis elegans. in this study the fmrfamide-related peptide (farp) kpsfvrfamide (879.90 da [mh]+) was structurally characterized from extracts of the nematode, caenorhabditis elegans. two copies of kpsfvrfamide are encoded by a gene designated flp-9. rt-pcr identified a single cdna product which was confirmed as flp-9 by sequence determination. flp-9 cdna was isolated from ...	1999	9920762
natural immunity to ascaris lumbricoides associated with immunoglobulin e antibody to aba-1 allergen and inflammation indicators in children.	children putatively immune to the large roundworm ascaris lumbricoides were identified in an area of nigeria where infection is hyperendemic. immunity was associated with higher levels of serum ferritin, c-reactive protein, and eosinophil cationic protein, indicating ongoing acute phase or inflammatory processes. in contrast, children who were susceptible to the infection had little serological evidence of inflammation despite their high parasite burdens. immunoglobulin g (igg) antibody activity ...	1999	9916049
distribution of ascaris suum in experimentally and naturally infected pigs and comparison with ascaris lumbricoides infections in humans.	this paper describes the distribution of ascaris suum in experimentally and naturally infected pigs, and offers a comparison with a. lumbricoides infections in humans. in the first study, worms were recovered post-mortem from a group of 38 pigs that had been trickle inoculated with 10,000 infective a. suum eggs twice weekly for 12 weeks. in the second study, worms were collected from a group of 49 pigs that had been kept on a pasture contaminated with infective a. suum eggs for 10 weeks, after w ...	1998	9881384
solution structure of the motile major sperm protein (msp) of ascaris suum - evidence for two manganese binding sites and the possible role of divalent cations in filament formation.	the major sperm protein (msp) of ascaris suum mediates amoeboid motility by forming an extensive intermeshed system of cytoskeletal filaments analogous to that formed by actin in many other amoeboid cells. msp is a dimeric molecule that polymerizes to form non-polar filaments constructed from two helical subfilaments that wind round one another. moreover, msp filaments can interact with one another to form higher-order assemblies without requiring the range of accessory proteins usually employed ...	1998	9878374
prevalence of anti-toxocara antibodies in a random sample of inpatients at a children's hospital in vitória, espírito santo, brazil.	in the streets of vitória, in the state of espírito santo, brazil, are large number of stray dogs, many of which are infected with toxocara canis, suggesting a high risk for human infection. in order to investigate the prevalence of toxocara infection in children in espírito santo we studied the prevalence of anti-toxocara antibodies in 100 random inpatients over one year of age, at the children's hospital n.s. da glória, the reference children's hospital for the state. all the sera were collect ...	1998	9876441
comparative study of histamine release from skin mast cells dispersed from atopic, ascaris-sensitive and healthy dogs.	atopic dermatitis results from the interaction between allergen and allergen-specific ige bound to the mast cell surface receptors. this process triggers mast cell degranulation and accounts at least for early phase reaction. furthermore, there is increasing in vitro and in vivo evidence that ige has the ability to induce overexpression of the fc epsilonri receptor on the mast cell plasma membrane. in order to study the potential effect of an increase in serum ige on mast cell activity, the hist ...	1998	9847019
purification and characterisation of a secreted aminopeptidase from adult ascaris suum.	a metalloaminopeptidase was identified in culture fluids collected during in vitro cultivation of adult ascaris suum. the enzyme was purified by anion-exchange and size-exclusion hplc. the m(r) of the enzyme was estimated at 293 kda and consisted of subunits with m(r)s of 153 and 142kda. the isoelectric point of the aminopeptidase was 4.7. the aminopeptidase displayed a substrate preference for terminal arginyl residues. aminopeptidase activity was also present in muscle, female reproductive tis ...	1998	9846604
the immunosuppressive activity of ascaris suum is due to high molecular weight components.	the role of isolated components obtained by gel filtration chromatography of ascaris suum body extract (asc) on the modulation of the immune response to ovalbumin (ova) was evaluated and correlated with the immunogenic properties of such components. we showed that high (pi), but not low (piii), molecular weight components have the ability to inhibit ova-induced immediate and dth reactions, lymph node (ln) cell proliferation, cytokine (il-2, interferon-gamma (ifn-gamma), il-4 and il-10) and antib ...	1998	9822283
primary sequence and activity analyses of a catalase from ascaris suum.	a complete cdna encoding the catalase (ec 1.11.1.6) has been isolated from the parasitic nematode ascaris suum (ascat). the active-site residues, the residues involved in ligand interaction, and nadph-binding residues of the bovine liver catalase-type enzyme are highly conserved in the ascat predicted amino acid sequence. to confirm that the ascat cdna encodes a functional enzyme, active recombinant protein (rascat) was produced in a procaryotic expression system. the subunit molecular mass of t ...	1998	9803413
immuno-cross-reactivity of cut-1 and cuticlin epitopes between ascaris lumbricoides, caenorhabditis elegans, and heterorhabditis.	cuticlin is the insoluble residue of nematode cuticle. it has been proved that cuticlin and cut-1-like epitopes are conserved between the free-living caenorhabditis elegans and the entomopathogenic nematode heterorhabditis sp. the cloning of a cut-1 homologous gene from the animal intestinal parasite ascaris lumbricoides has allowed us to extend the study of immuno-cross-reactivity at the ultrastructural level to this important species. antibodies against recombinant cut-1 protein and against cu ...	1998	9774538
the mitochondrial genome of onchocerca volvulus: sequence, structure and phylogenetic analysis.	the complete dna sequence of the mitochondrial genome of onchocerca volvulus is described. the o. volvulus mitochondrial genome is 13747 bp, slightly smaller than the mitochondrial genomes of the nematodes ascaris suum and caenorhabditis elegans, and the smallest metazoan mitochondrial dna molecule reported to date. the o. volvulus mitochondrial genome contains genes for two ribosomal rnas, 22 transfer rnas and 12 proteins. consistent with the small size of the genome, four gene pairs overlap an ...	1998	9763293
relative concentrations of heavy metals in the parasites ascaris suum (nematoda) and fasciola hepatica (digenea) and their respective porcine and bovine definitive hosts.	the concentrations of lead and cadmium determined by electrothermal atomic absorption spectrometry were significantly higher in the liver and kidney, respectively, of pigs than in their intestinal nematode parasites ascaris suum. there was no clear pattern in the distribution of lead within the ascarids, but cadmium concentrations were highest in the intestine. a parallel investigation of cattle naturally infected with the liver fluke, fasciola hepatica, revealed interesting differences. althoug ...	1998	9762561
[the effectiveness of different disinfectants based on p-chloro-m-cresol against ascaris suum eggs under laboratory conditions].	the devitalizing effectiveness of 5 patterns of the disinfectant neopredisan (active principle: p-chlorine-m-cresol) was tested to eggs of ascaris suum in coincidence with the "guidelines for the testing of chemical disinfectants" of the german veterinary society (gvs). in the suspension-test all examined patterns indicated a 100% inhibition of development ascaris suum-eggs. in the germ carrier test all patterns were in conformity with the necessities of the gvs-guidelines. also in the modified ...	1998	9741186
assembly and architecture of invertebrate cytoplasmic intermediate filaments reconcile features of vertebrate cytoplasmic and nuclear lamin-type intermediate filaments.	the two major intermediate filament (if) proteins from the esophagus epithelium of the snail helix pomatia and the two major if proteins from muscle tissue of the nematode ascaris suum were investigated under a variety of assembly conditions. the lowest-order complexes from each of the four protostomic invertebrate (p-inv) if proteins are parallel, unstaggered dimers involving two-stranded alpha-helical coiled coil formation of their approximately 350 amino acid residue central rod domain (i.e. ...	1998	9737925
budesonide affects allergic mucociliary dysfunction.	airway inflammation characterized by neutrophils and free elastase contributes to allergic mucociliary dysfunction. glucocorticosteroids are the most important anti-inflammatory agents used in the treatment of asthma, but their effect on allergic mucociliary dysfunction is not known. therefore, we assessed both the prophylactic and therapeutic effects of the glucocorticosteroid budesonide on antigen-induced mucociliary dysfunction in sheep. tracheal mucus velocity (tmv), a marker of mucociliary ...	1998	9729587
effect of protease class-specific inhibitors on in vitro development of the third- to fourth-stage larvae of ascaris suum.	third-stage larvae (l3) of ascaris suum develop and molt to fourth-stage larvae (l4) during in vitro cultivation; consistently greater than 80% of the larvae develop to l4 during 7 days in culture (dic). to assess the role of proteases in this process, the effect of protease class-specific inhibitors was studied. the presence of either a serine protease inhibitor (aebsf, 100 microm) or an aspartic protease inhibitor (pepstatin a, 100 microm) had no effect on the percentage of l4 after 7 dic. how ...	1998	9714194
immunocytochemical localization of a putative inhibitory amino acid receptor subunit in the parasitic nematodes haemonchus contortus and ascaris suum.	a rabbit antiserum was raised against a synthetic peptide corresponding to a region near the n-terminus of the haemonchus contortus inhibitory amino acid receptor subunit, hg1. the antiserum recognized a recombinant form of the n-terminal domain of the subunit on western blots and reacted with the ventral nerve cord of h. contortus in immunofluorescence experiments. immunofluorescence was also detected in specific head neurons of h. contortus: these were tentatively identified as ring motor- and ...	1998	9695104
dna extraction from ascaris suum muscle tissue.	a new method for the extraction of dna from ascaris suum muscle has been developed. it combines a standard sds-based extraction with a plant dna extraction procedure. the use of sds and proteinase k allows the elimination of proteins, while ctab and polyclar at eliminate glycogen and polyphenols. the dna thus obtained can easily be digested by endonucleases and amplified by pcr.	1998	9694380
natural ascaris suum infections in swine diagnosed by coprological and serological (elisa) methods.	paired samples of faeces and blood were collected from weaners (w), fatteners (f), lactating sows (s) and piglets (p) in 20 danish sow herds. the samples were examined by a mcmaster technique for ascaris suum eggs and an indirect elisa for anti-a. suum igg. the coprological and serological results were significantly correlated for w and f (p < 0.0001) but not for s (p = 0.35). the coproprevalences were much lower (w 4.0%, f 15.5%, s 7.4%) than the seroprevalences (w 20.3%, f 50.5%, s 65.4%). thu ...	1998	9694368
single-strand conformation polymorphism (sscp)-based mutation scanning approaches to fingerprint sequence variation in ribosomal dna of ascaridoid nematodes.	in this study, we assessed single-strand conformation polymorphism (sscp)-based approaches for their capacity to fingerprint sequence variation in ribosomal dna (rdna) of ascaridoid nematodes of veterinary and/or human health significance. the second internal transcribed spacer region (its-2) of rdna was utilised as the target region because it is known to provide species-specific markers for this group of parasites. its-2 was amplified by pcr from genomic dna derived from individual parasites a ...	1998	9694283
structural analysis and immunohistochemical localization of two acidic glycosphingolipids from the porcine, parasitic nematode, ascaris suum.	the acidic glycolipid fraction (af) of the porcine, parasitic nematode, ascaris suum , consisted of two subfractions. the major component af ii reacted with orcinol-sulfuric acid and molybdate, while the minor component af i gave a positive reaction with azure-a, a cationic dye specific for sulfatides. sugar constituent analysis, methanolysis, methylation analysis, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, liquid secondary-ion mass spectrometry, and gas-liquid ...	1998	9675222
strategies for preliminary characterization of novel amphoteric glycosphingolipids.		1998	9664355
the role of camp in the actions of the peptide af3 in the parasitic nematodes ascaris suum and ascaridia galli.	af3 (avpgvlrfamide) is an endogenous rfamide-like peptide isolated from the parasitic nematode ascaris suum. it has a potent and long lasting excitatory effect in a. suum and ascaridia galli. this is mediated by a mechanism independent of the nicotinic-like acetylcholine (ach) receptor, which mediates excitatory transmission at the neuromuscular junction of both nematodes. in addition, af3 has been found to sensitise a. suum muscle to the contractile effect of ach. in this study, the involvement ...	1998	9662710
biophysical model for organic acid excretion in ascaris suum.	to develop a model for the mechanisms of organic acid excretion in nematodes, we measured the concentrations of volatile fatty acids (vfas), ph and electrical potentials across hypodermal and muscle membranes and across the composite body wall (consisting of hypodermis, muscle and cuticle) of ascaris suum using standard chromatographic and microelectrode recording techniques. in incubates containing one parasite in 20 ml modified ascaris ringer's solution, the level of combined vfas excreted int ...	1998	9662703
cross-reactions between toxocara canis and ascaris suum in the diagnosis of visceral larva migrans by western blotting technique.	visceral larva migrans (vlm) is a clinical syndrome caused by infection of man by toxocara spp, the common roundworm of dogs and cats. tissue migration of larval stages causes illness specially in children. because larvae are difficult to detect in tissues, diagnosis is mostly based on serology. after the introduction of the enzyme-linked immunosorbent assay (elisa) using the larval excretory-secretory antigen of t. canis (tes), the diagnosis specificity was greatly improved although cross-react ...	1997	9661302
an outbreak of ascariasis with marked eosinophilia in the southern part of kyushu district, japan, caused by infection with swine ascaris.	ascariasis has been a representative soil-transmitted intestinal parasitic disease in warm climates. in japan, this disease was a major and serious public health problem only a few decades ago. however, the incidence of the disease nowadays is reportedly less than 0.01%. recently in 1994 through 1995, we experienced a total of 14 cases who were suspected as having ascariasis. they were characterized by peripheral blood eosinophilia (30-70%), high serum titers against ascaris antigen, and most no ...	1997	9656376
molecular cloning, expression and characterization of an ascaris inhibitor for pepsin and cathepsin e.	molecular cloning of a cdna for a pepsin inhibitor in the round worm, ascaris suum, was achieved. the orf was found to encode a 20-residue potential signal peptide and a 149-residue inhibitor moiety. northern analysis showed the mrna for the inhibitor to be expressed in the body wall and not in the viscera. to obtain the active inhibitor, we constructed a yeast expression vector, pyes2api, containing the inducible galactosidase promoter and a dna fragment encoding a fusion protein of the yeast a ...	1998	9654082
intestinal parasites in swine in the nordic countries: prevalence and geographical distribution.	in denmark (dk), finland (fin), iceland (i), norway (n), and sweden (s), 516 swine herds were randomly selected in 1986-1988. individual faecal analyses (mean: 27.9 per herd) from eight age categories of swine showed that ascaris suum, oesophagostomum spp., isospora suis, and eimeria spp. were common, while trichuris suis and strongyloides ransomi-like eggs occurred sporadically. large fatteners and gilts were most frequently infected with a. suum with maximum prevalences of 25-35% in dk, n and ...	1998	9650867
stable isotope-edited nmr analysis of ascaris suum mitochondrial trnamet having a tv-replacement loop.	most nematode mitochondrial (mt) trnas have a tv-replacement loop (tv loop) which replaces the normal t arm and the variable loop in standard trnas with a less-structured loop. the tertiary structure of such trnas has been discussed theoretically with reference to the crystal structure of yeast trnaphe [wolstenholme et al. (1994) nucleic acids res. 22, 4300-4306] and examined experimentally by chemical and enzymatic probing [watanabe et al. (1994) j. biol. chem. 269, 22902-22906]. the results su ...	1998	9644241
identification of protein-protein interactions of the major sperm protein (msp) of caenorhabditis elegans.	in nematodes, sperm are amoeboid cells that crawl via an extended pseudopod. unlike those in other crawling cells, this pseudopod contains little or no actin; instead, it utilizes the major sperm protein (msp). in vivo and in vitro studies of ascaris suum msp have demonstrated that motility occurs via the regulated assembly and disassembly of msp filaments. filaments composed of msp dimers are thought to provide the motive force. we have employed the yeast two-hybrid system to investigate msp-ms ...	1998	9641981
studies on the survival of ascaris suum eggs under laboratory and simulated field conditions.	a series of four experiments was carried out to study the survival of ascaris suum eggs: in a pig slurry unit on a farm, in the laboratory under anaerobic conditions and different relative humidities (rh), and under simulated field conditions. survival of eggs in the pig slurry unit was 20% after four weeks and 0% after 16 weeks. anaerobic conditions had only a minor influence on survival: after 12 weeks, more than 80% of the eggs could still develop. at high relative humidities (100% and 75%), ...	1998	9637224
embryonation and infectivity of ascaris suum eggs isolated from worms expelled by pigs treated with albendazole , pyrantel pamoate, ivermectin or piperazine dihydrochloride.	the effect of anthelmintic treatment of pigs on the embryonation and infectivity of ascaris suum eggs isolated from expelled worms was investigated. four groups of two naturally infected pigs were dosed with albendazole, pyrantel pamoate, ivermectin or piperazine dihydrochloride, respectively. following worm expulsion, the eggs were removed from the uteri of female worms and embryonated in sulphuric acid. the infectivity of the embryonated eggs was tested through mouse inoculation. egg developme ...	1998	9637219
anisakis simplex: mutational bursts in the reactive site centers of serine protease inhibitors from an ascarid nematode.		1998	9635450
detection of sequence variation in parasite ribosomal dna by electrophoresis in agarose gels supplemented with a dna-intercalating agent.	this study evaluated the use of a commercially available dna intercalating agent (resolver gold) in agarose gels for the direct detection of sequence variation in ribosomal dna (rdna). this agent binds preferentially to at sequence motifs in dna. regions of nuclear rdna, known to provide genetic markers for the identification of species of parasitic ascarid nematodes (order ascaridida), were amplified by polymerase chain reaction (pcr) and subjected to electrophoresis in standard agarose gels ve ...	1998	9629896
allergen challenge increases cell traffic between bone marrow and lung.	increases in inflammatory-cell progenitors have been demonstrated in the bone marrow (bm) after inhalation of ascaris suum in dogs at the time of allergen-induced airway hyperresponsiveness (ahr). the aim of this study was to evaluate the effect of allergen challenge on trafficking of inflammatory cells and their progenitors from the bm to the lung, using a marker of proliferating cells, bromodeoxyuridine (brdu). brdu is a thymidine analogue taken up by the dna of dividing cells, and can be dete ...	1998	9618380
[a case of pulmonary infiltration with eosinophilia caused by ascaris suum].	a 46-year old man was admitted to our hospital with dry cough and diarrhea. his chest ct showed diffuse subpleural small nodular shadows. eosinophilia and an increase of serum ige were detected. eosinophils were also increased in balf. an antibody against ascaris suum was positive in serum while other causes of eosinophilia (e. g., drugs, fungus, collagen disease) were absent. ivermectin was administered and the diarrhea improved within 3 days. the abnormal chest shadows and dry cough disappeare ...	1998	9617152
action of excitatory amino acids on hypodermis and the motornervous system of ascaris suum: pharmacological evidence for a glutamate transporter.	electrophysiological and pharmacological experiments suggest the presence of an electrogenic glutamate transporter in the motornervous system of the parasitic nematode ascaris suum. this putative transporter occurs in hypodermis (a tissue in some respects analogous to glia) and in de2 motorneurons, a dorsal excitatory motorneuron class which receives excitatory glutamatergic post-synaptic potentials. glutamate application to hypodermis produced non-conductance mediated depolarizations that were ...	1998	9614331
neurophysiology of glutamatergic signalling and anthelmintic action in ascaris suum: pharmacological evidence for a kainate receptor.	electrophysiological and pharmacological techniques were used to study glutamatergic signalling in the parasitic nematode, ascaris suum. glutamate or kainate injections into whole worms produced a paralysed quasi-static posture similar to the waveform in behaving worms. the de2 motorneuron class is a primary target. several glutamatergic substances produced pronounced conductance increases and depolarization in de2; domoate and kainate were the most potent agonists tested. glutamate responses an ...	1998	9614330
an in-vitro test for assessing the viability of ascaris suum eggs exposed to various sewage treatment processes.	a simple procedure using ascaris suum as a model for ascaris lumbricoides in testing the ability of sludge treatment processes to kill the eggs of parasitic roundworms was developed. unembryonated and embryonated a. suum eggs were placed in a mesophilic anaerobic sludge digester or a sludge lagoon, or stored at 4 degrees c. unembryonated eggs were recovered and incubated at 32.5 degree c and 90-95% r.h. for 48 h and viable eggs developed embryos consisting of two or more clearly defined cells. t ...	1998	9602387
expression of pyruvate dehydrogenase isoforms during the aerobic/anaerobic transition in the development of the parasitic nematode ascaris suum: altered stoichiometry of phosphorylation/inactivation.	the pyruvate dehydrogenase complex (pdc) plays a key role in the anaerobic metabolism of the parasitic nematode ascaris suum. two isoforms of the alpha-subunit of pyruvate dehydrogenase (e1) have been identified: alpha i is most abundant in anaerobic adult muscle and alpha ii in aerobic larvae. both isoforms have been expressed as alpha 2 beta 2 tetramers with a muscle-specific beta-subunit, purified to apparent homogeneity, reconstituted with e1-deficient adult a. suum muscle pdc, and assayed f ...	1998	9587415
molecular cloning, functional expression, and characterization of pyruvate dehydrogenase kinase from anaerobic muscle of the parasitic nematode ascaris suum.	the pyruvate dehydrogenase complex (pdc) plays a key role in the anaerobic mitochondrial metabolism of the parasitic nematode ascaris suum. a cdna coding for an a. suum pyruvate dehydrogenase kinase (apdk) has been cloned and sequenced from poly(a)+ rna isolated from adult a. suum muscle.2 apdk exhibited significant sequence identity to mammalian pdks. nucleotide sequence analysis of the apdk cdna revealed a 22-nucleotide spliced leader, characteristic of many nematode mrnas, a 5'-utr of 6 nucle ...	1998	9578613
growth and structural features of the adult stage of ascaris suum (nematoda, ascaridoidea) from experimentally infected domestic pigs.	for studying the morphogenesis of the adult stage of ascaris suum, worms were obtained from experimentally infected domestic pigs. structural and morphometric features of a. suum are analyzed by light and scanning electron microscopy. it was shown that the increase in length of the worm from days 35 to 56 is based on a region-specific lengthening of individual transverse annuli in the cuticle. an increased worm size also results in an increased diameter of different cuticular sense organs. it is ...	1998	9576498
developmental and tissue-specific expression of 2-methyl branched-chain enoyl coa reductase isoforms in the parasitic nematode, ascaris suum.	the 2-methyl branched-chain enoyl coa reductase (ecr) plays a pivotal role in the reversal of beta-oxidation operating in anaerobic mitochondria of the parasitic nematode, ascaris suum. two-dimensional gel electrophoresis of the purified ecr yielded multiple spots, with two distinct but overlapping n-terminal sequences. these multiple isoforms were not the result of population effects, as the pattern observed on 2-d gels of the purified ecr was identical to those on immunoblots of muscle homogen ...	1998	9566523
use of polymerase chain reaction in the diagnosis of toxocariasis: an experimental study.	in this paper we report the usefulness of polymerase chain reaction technique in the diagnosis of visceral larva migrans in a mouse model. liver samples obtained from two set of experimentally infected mice (10, 100, 1,000 and 10,000 embryonated toxocara canis eggs per mouse) along with the eggs of t. canis, t. cati and ascaris suum were included in this study. polymerase chain reaction (pcr) was performed using toxocara primers (sb12). the first pcr product electrophoresis revealed very thin po ...	1997	9561605
x-ray crystallographic studies of the complex between porcine pepsin and the aspartic proteinase inhibitor pi-3 from the nematode ascaris suum.		1998	9561247
expression, purification, and characterization of the recombinant pepsin inhibitor from ascaris suum.		1998	9561246
differential effects of cyclosporine a after acute antigen challenge in sensitized cats in vivo and ex vivo.	1. we determined the effect of cyclosporine a (csa) treatment on mast cell degranulation and lung resistance (r(l)) in vivo, and tracheal smooth muscle (tsm) contraction ex vivo after antigen challenge in sensitized cats. we also determined the direct effects of addition of csa to the tissue bath on antigen-induced responses of tsm in vitro. 2. cats (n=10) were sensitized by i.m. injection of ascaris suum antigen (aa); 5 cats (csa+) received csa twice daily for 2 weeks before acute antigen chall ...	1998	9559905
modulation of the motility of the vagina vera of ascaris suum in vitro by fmrf amide-related peptides.	ascaris suum contains a large number of fmrfamide-related peptides (farps) of which knefirfamide (af1), kheylrfamide (af2) and ksaymrfamide (af8, also called pf3) have been extensively studied and are known to exert actions on somatic muscle strips of the worm. in the present study, the effects of af1, af2 and af8 on the activity of the vagina vera of female a. suum have been examined in vitro. the vagina vera is a muscular tube connecting the uterus and vagina uteri to the gonopore and is proba ...	1998	9550221
ascaris suum: protein phosphotyrosine phosphatases in oocytes and developing stages.	protein tyrosine phosphatases were analyzed in oocytes of ascaris suum. phosphatases dephosphorylating modified acidic lysozyme were present in high-molecular-weight form (m(r) > 600,000) and as a 50- to 55-kda protein in the soluble fraction. the low-molecular-weight form of the phosphatase cross-reacted with an antiserum raised against human t-cell protein tyrosine phosphatase and was not distinguishable from the 50- to 55-kda protein tyrosine phosphatase previously described in the muscular l ...	1998	9538868
inhibition of antigen-induced airway hyperresponsiveness by ultralow molecular-weight heparin.	unfractionated heparin (uf-heparin) has been shown to prevent antigen-induced airway hyperresponsiveness (ahr), but it is ineffective when administered after the antigen challenge. we hypothesized that the failure of uf-heparin to modify postantigen ahr might depend on molecular weight. we therefore studied the effects of uf-heparin and three low-molecular-weight heparin fractions (medium-molecular-weight heparin [mmwh]; low-molecular-weight heparin [lmwh]; and ultralow-molecular-weight heparin ...	1998	9517607
effect of chronic antigen inhalation in guinea pigs.	to examine the role of airway inflammation in airway hyperresponsiveness (ahr), we examined the effect of chronic antigen inhalation in sensitized guinea pigs. guinea pigs were actively sensitized with dinitrophenylated ascaris suum extract (dnp-as) and repeatedly exposed to aerosolized dnp-as antigen once a day for 4 or 10 days. twenty-four hours after the last antigen exposure, airway responsiveness to inhaled acetylcholine (ach) and bronchoalveolar lavage (bal) were studied. the guinea pigs r ...	1998	9513585
cloning and characterization of a putative testis-specific pyruvate dehydrogenase beta subunit from the parasitic nematode, ascaris suum.		1997	9497067
altered phosphorylation/inactivation of a novel pyruvate dehydrogenase in adult ascaris suum muscle.		1997	9497054
seroepidemiology of abdominal angiostrongyliasis: the standardization of an immunoenzymatic assay and prevalence of antibodies in two localities in southern brazil.	abdominal angiostrongyliasis is a nematode disease produced by angiostrongylus costaricensis, a metastrongylid parasite of wild rodents. accidental human infection occurs through ingestion of food or water contaminated with third-stage larvae present in the mucous secretion of terrestrial molluscs. an elisa test was standardized for detection of igg antibodies recognizing a surface antigen prepared from female worms. competitive absorption of sera with ascaris suum crude antigen resulted in a te ...	1997	9491104
early ascaris suum migration in mice as a model for pigs.	a study was made of the early migratory pattern of ascaris suum in mice. mice were each inoculated orally with a single dose of 2,500 infective eggs and then killed at 2, 4, 6, 8, 10, and 12 hr postinoculation (pi). at necropsy, it was observed that the larvae had penetrated the mouse cecum and colon and had reached the liver by 4 hr pi. this migratory behavior closely mimics what is observed in the pig and suggests that the mouse may serve as an experimental model for intestinal immunity in the ...	1998	9488331
release of epithelium-derived pge2 from canine trachea after antigen inhalation.	to investigate the role of prostaglandin (pg) e2 in allergen-induced hyperresponsiveness, dogs inhaled either the allergen ascaris suum or vehicle (sham). twenty-four hours after inhalation, some animals exposed to allergen demonstrated an increased responsiveness to acetylcholine challenge in vivo (hyp-resp), whereas others did not (non-resp). strips of tracheal smooth muscle, either epithelium intact or epithelium denuded, were suspended on stimulating electrodes, and a concentration-response ...	1998	9486206
ascaris suum prevalence and intensity: an abattoir survey of market hogs in saskatchewan.	two surveys examining the prevalence and intensity of ascaris suum were conducted at a saskatchewan abattoir. in the first, 2500 livers from market weight animals were examined and scored for lesions consistent with ascarid migration. forty-four percent displayed some degree of scarring with approximately 8% being severely damaged. in the second, both livers and intestinal contents from 500 market weight animals were examined. seventeen percent were infected with intestinal a. suum (mean of 2.5 ...	1997	9477517
helminth surveillance as a prerequisite for anthelmintic treatment in intensive sow herds.	helminth prevalences in pigs in intensive production systems are often very low due to housing and management factors, and the present study was carried out to examine whether routine anthelmintic treatment in such herds can be replaced by coprological surveillance of the helminth status combined with anthelmintic treatment when necessary. after an initial examination, 25 out of 28 large herds of danish sows (> or = 50 sows, production of fatteners) were found to have such light helminth infecti ...	1997	9477500
immunomodulation induced by ascaris suum extract in mice: effect of anti-interleukin-4 and anti-interleukin-10 antibodies.	simultaneous immunization of mice with an ascaris suum extract (asc) and ovalbumin (oa) markedly affects the immune response to oa. the role of interleukin (il)-4 and il-10 induced by asc immunization on the modulation of antigen-specific and mitogen-induced responses was investigated following single or combined cytokine-specific monoclonal antibody (moab) treatment of mice before immunization with oa + asc. immediate hypersensitivity reactions to aggregated oa and oa-specific immunoglobulin (i ...	1998	9467652
inhibition of allergic airway responses by inhaled low-molecular-weight heparins: molecular-weight dependence.	inhaled heparin prevents antigen-induced bronchoconstriction and inhibits anti-immunoglobulin e-mediated mast cell degranulation. we hypothesized that the antiallergic action of heparin may be molecular weight dependent. therefore, we studied the effects of three different low-molecular-weight fractions of heparin [medium-, low-, and ultralow-molecular-weight heparin (mmwh, lmwh, ulmwh, respectively)] on the antigen-induced acute bronchoconstrictor response (abr) and airway hyperresponsiveness ( ...	1998	9451639
effects of environmental pollutants on the porcine and bovine immune systems.	immunological examinations were done in 30 fattening pigs from three swine farms (d., m., t.) and 20 dairy cows from one cattle farm (n.) in the district of hodonín in 1994. at the same time, samples of stable dust deposits collected in the four farms were analysed for the contents of mercury, cadmium, lead, gamma-hch (lindane), and total ddt and polychlorinated biphenyls (pcb). decreased blood level of lysozyme (0.92 mg/l) was found in the swine herd d. ten percent of the pigs in the herd were ...	1997	9446971
use of an agar-gel technique for large scale application to recover ascaris suum larvae from intestinal contents of pigs.	four groups each of 3 pigs were inoculated with ascaris suum eggs. pigs in groups 1 and 3 were inoculated with 1000 eggs, and pigs in groups 2 and 4 with 10,000 eggs. on day 10 and 21 post-inoculation (p.i.), respectively, groups 1 + 2 and 3 + 4 were slaughtered, and the contents from the small intestines collected. the contents were mixed with agar to a final concentration of 1% agar and allowed to sediment. the larvae were allowed to migrate from the agar-gel into 38 degrees c 0.9% saline over ...	1997	9444774
a novel uv-damaged dna binding protein emerges during the chromatin-eliminating cleavage period in ascaris suum.	during the early cleavage period of ascaris suum , chromatin diminution takes place in the somatic founder cells. in the process of chromatin diminution numerous heterochromatic blocks, consisting predominantly of highly repeated dna, are discarded during mitotic anaphase and are later on digested in the cytoplasm. very little is known about proteins that are involved in chromatin diminution. we have detected a nuclear protein and purified it to near homogeneity by its preferential binding to uv ...	1998	9443969
effects of concurrently administered copper and mercury on phagocytic cell activity and antibody levels in guinea pigs with experimental ascariasis.	the subchronic effect of copper and mercury on selected immunological parameters in guinea pigs with experimental ascariasis was studied. cupric sulphate and mercuric chloride administered for 28 days did not suppress the levels of specific circulating antibody in a subsequent ascaris suum infection. intoxication of animals significantly inhibited the phagocytic activity of peritoneal macrophages throughout the experiment. ascaris suum infection elevated macrophage phagocytic activity but values ...	1997	9443953
hydrostatic pressure shows that lamellipodial motility in ascaris sperm requires membrane-associated major sperm protein filament nucleation and elongation.	sperm from nematodes use a major sperm protein (msp) cytoskeleton in place of an actin cytoskeleton to drive their ameboid locomotion. motility is coupled to the assembly of msp fibers near the leading edge of the pseudopod plasma membrane. this unique motility system has been reconstituted in vitro in cell-free extracts of sperm from ascaris suum: inside-out vesicles derived from the plasma membrane trigger assembly of meshworks of msp filaments, called fibers, that push the vesicle forward as ...	1998	9442112
myogenic and neurogenic mechanisms and arachidonate metabolites in bronchial muscle response to allergen.	we investigated allergen-induced airway hyperresponsiveness (ah) in bronchial tissues obtained from dogs that inhaled ascaris suum leading to ah (resp) in vivo or that exhibited no change (non-resp) as well as from dogs that inhaled saline (sham). resp tissues were not hyperresponsive to kcl or to carbachol, whereas contractions to electrical field stimulation (efs) were reduced. this reduction was reversed partially by indomethacin and completely by replacement of the bathing fluid. radioimmuno ...	1997	9435565
comparison of methods for recovery of ascaris suum larvae from tissues of mice.	experiments were undertaken to compare procedures for isolating ascaris suum from mice. a standardised intragastric procedure for inoculation of a. suum eggs, which had a very low interdose variation (s.d. = 6.2% of mean inoculation dose), was developed. there was no significant difference in the number of larvae recovered from the large intestine 4 h post inoculation (p.i.) by using either baermannisation of washed intestinal wall or submucosal scrapings. in contrast, a significantly higher rec ...	1997	9421715
structural elucidation and monokine-inducing activity of two biologically active zwitterionic glycosphingolipids derived from the porcine parasitic nematode ascaris suum.	the isolated neutral glycosphingolipid fraction from the pig parasitic nematode, ascaris suum, was fractionated by silica gel chromatography to yield a neutral and a zwitterionic glycosphingolipid fraction, the latter of which mainly contained two zwitterionic glycosphingolipids termed components a and c. preliminary chemical characterization with hydrofluoric acid treatment and immunochemical characterization with a phosphocholine-specific monoclonal antibody indicated that both components cont ...	1998	9417105
tachykinins contribute to the acute airways response to allergen in sheep actively sensitized to ascaris suum.	tachykinins, found in sensory nerves, have effects in the airways which suggest that they may contribute to the pathogenesis of asthma. we aimed to find evidence for tachykinin involvement in the immediate airway response to allergen in a sheep model of experimental asthma. twenty-four sheep were actively sensitized to ascaris suum, then challenged with nebulized ascaris extract in a dose-response fashion. change in lung resistance (rl) in response to challenge was measured. responder sheep (tho ...	1997	9400681
secretion of an aminopeptidase during transition of third- to fourth-stage larvae of ascaris suum.	protease activity was identified in culture fluids collected during in vitro development of l3 to l4 larval stages of ascaris suum. fluorogenic peptide substrates with unblocked n-termini were specifically hydrolyzed indicating aminopeptidase activity; a terminal arginyl residue was preferred. culture fluids did not hydrolyze fluorogenic peptide substrates with blocked n-termini (endopeptidase substrates). the aminopeptidase activity was inhibited by 1,10-phenanthroline (metalloprotease inhibito ...	1997	9379278
determination of airway and tissue resistances after antigen and methacholine in nonhuman primates.	antigen challenge of ascaris suum-sensitive animals has been used as a model of asthma in humans. however, no reports have separated total respiratory resistance into airway (raw) and tissue (rti) components. we compared input impedance (zin) and transfer impedance (ztr) to determine raw and rti in anesthetized cynomolgus monkeys under control and bronchoconstricted conditions. zin data between 1 and 64 hz are frequency dependent during baseline conditions, and this frequency dependence shifts i ...	1997	9375340
role of circulating blood components and thromboxane in anaphylactic vasoconstriction in isolated canine lungs.	we determined the roles of circulating blood components and chemical mediators in anaphylactic vasoconstriction and microvascular permeability during ascaris suum antigen-induced anaphylaxis in isolated canine lungs. either the right or left lower lobe served as the control lung, which was perfused with autologous blood, and the contralateral lobe from the same dog was examined for the effect of albumin krebs-henseleit solution (krebs) or of blockers of various vasoconstrictors with blood perfus ...	1997	9375313
inhibitory effects of nematode fmrfamide-related peptides (farps) on muscle strips from ascaris suum.	a large number of fmrfamide-related peptides (farps) are found in nematodes, and some of these are known to influence tension and contractility of neuromuscular strips isolated from ascaris suum body wall. relaxation of these strips has been noted with five nematode farps. the inhibitory actions of sdpnflrfamide (pf1) and sadpnflrfamide (pf2) appear to be mediated by nitric oxide, as previously demonstrated with inhibitors of nitric oxide synthase (nos). this present study showed that the effect ...	1995	9372147
serological assays for the identification of oesophagostomum dentatum infections in pigs.	oesophagostomum dentatum antigen preparations of third (l3) or fourth (l4) stage larvae were characterised by western blotting. panels of sera obtained from pigs infected with o dentatum and ascaris suum, respectively, reacted with the same bands of l3 antigen. in contrast high specificity against a characteristic band, was observed when l4 extract was employed as antigen. to establish an enzyme-linked immunosorbent assay (elisa), a panel of homologous and heterologous sera was tested against o ...	1997	9368949
actions of the anthelmintic ivermectin on the pharyngeal muscle of the parasitic nematode, ascaris suum.	the anthelmintic invermectin has a number of effects on nematodes which result in changes in behaviour, particularly locomotion, including paralysis and an inhibition of feeding. this paper describes the application of an in vitro pharmacological approach to further delineate the action of ivermectin on feeding behaviour. contraction of ascaris suum pharyngeal muscle was monitored using a modified pressure transducer system which detects changes in intrapharyngeal pressure and therefore contract ...	1997	9368907
purification and properties of a membrane aminopeptidase from ascaris suum muscle that degrades neuropeptides af1 and af2.	we have identified on the membranes of the locomotory muscle of ascaris suum an amastatin-sensitive aminopeptidase that hydrolyses the bioactive neuropeptides af1 (knefirf-nh2) and af2 (kheylrf-nh2), by cleavage of the lys1-asn2 and lys1-his2 peptide bonds, respectively. af2 (1.2 nmol of heylrf-nh2 formed min[-1] (mg protein[-1])) was hydrolysed at a faster rate compared to af1 (0.2 nmol of nefirf-nh2 formed min[-1] (mg protein[-1])). af1 hydrolysis by the aminopeptidase was inhibited by the ama ...	1997	9364967
experimental ascaris suum infection in the pig: worm population kinetics following single inoculations with three doses of infective eggs.	to study population kinetics during primary ascaris suum infections, 3 groups of 52 pigs each were inoculated with 100, 1000, or 10,000 infective eggs. in all groups, the majority of larvae was found in the liver on day 3 post inoculation (p.i.) and in the lungs on day 7 p.i. liver white spots, caused by migrating larvae, were most numerous at day 7 p.i., whereafter they gradually healed, and only low numbers of granulation-tissue type white spots and lymphonodular white spots persisted at days ...	1997	9364572
sex-manipulated ascaris suum infections in pigs: implications for reproduction.	in spite of the vast distribution and considerable impact on human and animal health of ascaris suum and a. lumbricoides, little is known of the sexual biology and reproductive capacity of these intestinal nematodes. by oral transfer of adult female worms to previously parasite-naive pigs we show that in the absence of males the egg production ceases after 2-3 weeks. such females readily resume egg production a few days after oral transfer of male worms. these observations throw light on an impo ...	1997	9364571
helminthosis in local and cross-bred pigs in the morogoro region of tanzania.	we investigated the prevalence, burden and types of gastro-intestinal helminths in 424 local and cross-bred pigs kept under different management systems in two climatic zones in the morogoro region of tanzania. coprological examination revealed that 53% of the pigs excreted helminth eggs in their faeces. the median eggs per gram of faeces (epg) was 500 (range 100-22,000). local breeds in the mgeta location with tropical highland climate showed significantly higher prevalence (p < 0.001) and medi ...	1997	9361319
afp-1: a gene encoding multiple transcripts of a new class of fmrfamide-like neuropeptides in the nematode ascaris suum.	we have identified a gene, afp-1, that encodes a new subfamily of six fmrfamide-like neuropeptides in the nematode ascaris suum. the predicted peptides share the c-terminal sequence pgvlrf-nh2 but have different n-terminal extensions. we discuss possible functional roles of these different peptides based upon experiments with ascaris as well as results from other organisms. three of the peptides were previously isolated from extracts of a. suum (4) and three other are novel sequences. the transl ...	1997	9357048
a comparison of functional and structural consequences of the tyrosine b10 and glutamine e7 motifs in two invertebrate hemoglobins (ascaris suum and lucina pectinata).	the architecture of the distal heme pocket in hemoglobins and myoglobins can play an important role in controlling ligand binding dynamics. the size and polarity of the residues occupying the distal pocket may contribute steric and dielectric effects. in vertebrate systems, the distal pocket typically contains a "distal" histidine at position e7 and a leucine at position b10. there are several invertebrate organisms that have hemoglobins or myoglobins that display a pattern in which residues e7 ...	1997	9335574
cloning, structural analysis, and expression of the pig ige epsilon chain.	as a step in the evolutionary studies of immunoglobulin e (ige) and for the purpose of developing new reagents that will facilitate a more detailed analysis of ige-mediated inflammatory reactions in a large animal model, we here present the cloning of the epsilon chain of ige in the domestic pig (sus scrufa). a partial cdna clone for the epsilon chain of pig ige was isolated by polymerase chain reaction (pcr) amplification using degenerate primers directed against conserved regions in the second ...	1997	9321425
blockade of late-phase airway responses and airway hyperresponsiveness in allergic sheep with a small-molecule peptide inhibitor of vla-4.	the leukocyte integrin very late antigen-4 (vla-4) (alpha 4 beta 1, cd49d/cd29) is an adhesion receptor predominantly expressed on lymphocytes, monocytes, and eosinophils, but not on neutrophils. recent studies with monoclonal antibodies against vla-4 suggest that antigen-induced late responses and airway hyperresponsiveness (ahr) may depend on the recruitment and/or activation of vla-4-expressing leukocytes. to further test this hypothesis, we administered by aerosol either a potent small-molec ...	1997	9309981
validation of the specificity of the modified agglutination test for toxoplasmosis in pigs.	serum samples from toxoplasma gondii-negative pigs infected with protozoa, helminths and viruses, and from fetal pigs were examined for cross reacting antibodies to t. gondii by the use of the modified agglutination test (mat). nonspecific reactions with t. gondii antigen were not found in 1:10 dilutions of sera from eight pigs experimentally infected with sarcocystis miescheriana, 11 pigs infected with ascaris suum, three pigs infected with trichuris suis, ten pigs infected with trichinella spi ...	1997	9299699
false-positive ascaris suum egg counts in pigs.	false-positive ascaris suum egg counts in pig faeces are frequently observed under both experimental and natural conditions. data from 12 experiments with a. suum infections in pigs were summarized and showed that the percentage of false-positive faecal samples ranged from 4 to 36%. false-positive egg count values varied greatly between pigs and experiments (range 20-1060 eggs per gram faeces). indoor experiments with pigs housed groupwise in pens generally produced more and higher false-positiv ...	1997	9279587
lactoferrin, a potent tryptase inhibitor, abolishes late-phase airway responses in allergic sheep.	tryptase, a serine protease released exclusively from activated mast cells, has been implicated as a potential causative agent in asthma. enzymatically active tryptase is comprised of four subunits, and heparin stabilizes the associated tetramer. lactoferrin, a cationic protein released from activated neutrophils, binds tightly to heparin, therefore we investigated lactoferrin as an inhibitor of tryptase and found that it is both a potent (ki' is 24 nm) and selective inhibitor. size exclusion ch ...	1997	9279212
a patch clamp study of a glutamatergic chloride channel on pharyngeal muscle of the nematode ascaris suum.	glutamatergic chloride channels on the pharyngeal muscle of ascaris suum could be activated with glutamate and ivermectin and reversibly blocked with picrotoxin using the patch clamp technique. no activation was observed with gaba, glycine and acetylcholine. most of the current was carried by the main subconductance state of 21 ps. two smaller subconductance states occurred rarely. open time histograms could be best fitted by two time constants of tau(o1) = 0.33 ms and tau(o2) = 9.8 ms present a ...	1997	9272691
survival of model helminth eggs and larvae (ascaris suum, oesophagostomum sp.) in the ensilaging process.	ascaris suum nonembryonated eggs remained viable for the most part even after 42 days of ensilaging. at the end of the anaerobic fermentation, mean of damaged eggs was 15.2 +/- 4.02 (min. 11, max. 21), 32.9%. conversely, the viability of oesophagostomum sp. nonembryonated eggs and infective l3 larvae was reduced-eggs: mean number 23.6 +/- 3.64 (min. 20. max. 28) specimens (93.3%), l3 larvae: mean number 24.2 +/- 4.38 (min. 19, max. 28) specimens (96.7%), during the period of study (42 days). con ...	1997	9269126
localization of cytochrome oxidase and the 2-methyl branched-chain enoyl coa reductase in muscle and hypodermis of ascaris suum larvae and adults.	rabbit lung-derived third-stage larvae (l3) of ascaris suum are aerobic and cyanide sensitive but also contain many enzymes specific to anaerobic pathways. to localize these enzymes, diaminobenzidine (dab) staining for cytochrome oxidase (cox) and immunogold labeling for 2-methylbutyryl enoyl coa reductase (ecr) were performed on sections of hypodermis and muscle of adults and larvae of a. suum and visualized by transmission electron microscopy. as predicted, adult hypodermal and muscle mitochon ...	1997	9267425
a myoglobin mutant designed to mimic the oxygen-avid ascaris suum hemoglobin: elucidation of the distal hydrogen bonding network by solution nmr.	the solution 1h nmr structure of the active site and ligand dissociation rate for the cyanomet complex have been determined for a sperm whale myoglobin triple mutant leu29(b10)-->tyr, his64(e7)-->gln, thr67(e10)-->arg that mimics the distal residue configuration of the oxygen-avid hemoglobin from ascaris suum. a double mutant that retains leu29(b10) was similarly investigated. two-dimensional nmr analysis of the iron-induced dipolar shifts, together with the conserved proximal side structure for ...	1997	9251819
increase of eosinophilic cell population in bone marrow of rats by immunization with ascaris suum antigen.	immunization of rats with the antigen, ascaris suum extract, increased the number of peripheral eosinophils. analysis by western blot and reverse transcription-polymerase chain reaction revealed that the levels of major basic protein and its mrna in the bone marrow were also increased, suggesting that eosinophilic cell population in the bone marrow is increased by the immunization. these findings indicate that immunization with this antigen stimulates differentiation of progenitor cells to eosin ...	1997	9246564
the time course of the specific antibody response by various elisas in pigs experimentally infected with toxoplasma gondii.	with the aim of developing routine serological tests for monitoring the toxoplasma infection status of danish swine herds, four elisas based on tachyzoite antigen were set up: (1) an indirect elisa for igg-antibody; (2) a blocking elisa for antibody to the membrane antigen, p30; (3) an indirect elisa for igm; (4) a reverse, antibody-catching igm-elisa. groups of pigs (number between 6 and 10) were inoculated with tachyzoites of the rh-strain, tissue cysts of two complete strains, or oocysts in t ...	1997	9231984
cytochromes in the respiratory chain of helminth mitochondria.	parasitic helminths exhibit greater diversity in energy metabolism than do the host animals and many have exploited unique respiratory chains as adaptations to their natural habitats. cytochromes are involved, not only in intracellular aerobic respiration found in free-living stages, but also in the reduction of relatively oxidized compounds such as fumarate during the adult stages of parasitic helminths. in addition, most helminths retain a significant capacity to produce energy via aerobic pat ...	1997	9229245
onchocerca volvulus: immunohistochemical and immunoelectron microscopical distribution of a polyamine oxidizing enzyme.	we studied the distribution of a polyamine oxidizing enzyme (pao) in onchocerca volvulus and other nematode parasites by immunohistochemistry and electron microscopy with immunogold technique using a polyclonal antiserum raised against purified pao from ascaris suum. in adult o. volvulus the protein was localized in the outer zone and the area of the basal labyrinth of the hypodermis and occasionally in the outer zone of the uterine epithelium. further, the fluid in the body cavity was strongly ...	1997	9217703
evidence for increased hydroxylation of pyrrolidone amino acid residues in the cuticle of mature onchocerca volvulus.	to determine whether morphologic changes are accompanied by variations in the biochemical and antigenic properties of the cuticle of onchocerca volvulus during development, we isolated and compared the 2-mercaptoethanol soluble cuticular proteins and the insoluble cuticlin from the predominant life-cycle stages occurring in man. sds-page analysis, before and after digestion with collagenase from achromobacter iophagus, revealed that the polypeptide composition of the 2-mercaptoethanol-solubilise ...	1997	9197461
isolation and partial characterization of a fatty-acid-binding protein from ascaris suum reproductive tissue.	a 12-kda fatty-acid-binding protein was purified to homogeneity from ascaris suum reproductive tissue as confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. n-terminal amino-acid-sequence analysis of the protein revealed its identity with the aba-1 allergen protein isolated from a. suum pseudocoelomic fluid. fatty-acid binding by the protein from a. suum reproductive tissue was investigated using the lipidex 1000 assay, which revealed the presence of a single class of fatty-a ...	1997	9197404
in vitro anthelmintic activity of root-tuber extract of flemingia vestita, an indigenous plant in shillong, india.	the in vitro activity of root-tuber-peel extract of flemingia vestita, an indigenous plant consumed by the natives in northeast india, was tested against helminth parasites. live parasites (nematode: ascaris suum from pigs, a. lumbricoides from humans, ascaridia galli and heterakis gallinarum from domestic fowl; cestode: raillietina echinobothrida from domestic fowl; trematode: paramphistomum sp. from cattle) were collected in 0.9 % physiological buffered saline (pbs) and maintained at 37 +/- 1 ...	1997	9197399