Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| antifungal resistance does not necessarily affect candida glabrata fitness. | although there has been an overall good coverage of candida glabrata infections by the echinocandins, emergence of antifungal resistance during therapy has been reported. we investigated, by using an invertebrate host model, the fitness of sequential c. glabrata isolates with different echinocandins susceptibility patterns. the studied strains were isolated from a case of recurrent fungemia with a fatal outcome due to c. glabrata that developed cross-resistance to echinocandins during caspofungi ... | 2014 | 24091025 |
| effects of bovine lactoferrin to oral candida albicans and candida glabrata isolates recovered from the saliva in elderly people. | the effects of bovine lactoferrin (blf) on the growth of candida species and on inflammatory cytokine production in gingival keratinocytes, ndusd-1 co-cultured with candida strains were investigated. the results showed that blf at 10 and 100 μg/ml significantly inhibits the growth of two c. albicans strains and two c. glabrata strains isolated from the saliva of elderly people requiring nursing care, respectively. the levels of inflammatory cytokines, interleukin (il)-6, and il-8 in ndusd-1 co-c ... | 2013 | 24077721 |
| intestinal agvhd and infection after hematopoietic stem cell transplantation. | we aimed to guide clinical nursing by studying the relationship between intestinal acute graft-versus-host disease and intestinal infection after hematopoietic stem cell transplantation. | 2013 | 24077343 |
| assessment of host defence mechanisms induced by candida species. | some species of candida are opportunistic pathogens that can cause disease in a host immunocompromised by underlying local or systemic pathological processes. c. albicans is the species most often associated with oral lesions, but other species of candida, including c. glabrata, c. tropicalis and c. parapsilosis, have also been isolated in the saliva of subjects with and without candidiasis. in the present study we evaluated the host defence mechanisms induced by candida albicans and other candi ... | 2017 | 24067462 |
| anidulafungin and micafungin mic breakpoints are superior to that of caspofungin for identifying fks mutant candida glabrata strains and echinocandin resistance. | by clsi interpretive criteria, anidulafungin and micafungin mics determined by various methods were sensitive (60 to 70%) and highly specific (94 to 100%) for identifying fks mutations among 120 candida glabrata isolates. anidulafungin and micafungin breakpoints were more specific than clsi's caspofungin breakpoint in identifying fks mutant strains and patients with invasive candidiasis who were likely to fail echinocandin treatment (p ≤ 0.0001 for both). echinocandin mics were most useful clini ... | 2013 | 24060873 |
| emergence of non-albicans candida among candidal vulvovaginitis cases and study of their potential virulence factors, from a tertiary care center, north india. | the purpose of this study was to determine the prevalence of various candida species and study some of their virulence factors among thevulvovaginal candidiasis(vvc)patients. | 2013 | 24056652 |
| elucidating features that drive the design of selective antifolates using crystal structures of human dihydrofolate reductase. | the pursuit of antimicrobial drugs that target dihydrofolate reductase (dhfr) exploits differences in sequence and dynamics between the pathogenic and human enzymes. here, we present five crystal structures of human dhfr bound to a new class of antimicrobial agents, the propargyl-linked antifolates (plas), with a range of potency (ic50 values of 0.045-1.07 μm) for human dhfr. these structures reveal that interactions between the ligands and asn 64, phe 31, and phe 34 are important for increased ... | 2013 | 24053334 |
| treatment of invasive candidiasis in the elderly: a review. | fungi are major causes of infections among immunocompromised or hospitalized patients with serious underlying diseases and comorbidities. candida species remain the most important cause of opportunistic infections worldwide, affecting predominantly patients over 65 years old, while they are considered to be the fourth most common cause of nosocomial bloodstream infections. the rapidly growing elderly population has specific physiological characteristics, which makes it susceptible to colonizatio ... | 2013 | 24043935 |
| comparative genomics of emerging pathogens in the candida glabrata clade. | candida glabrata follows c. albicans as the second or third most prevalent cause of candidemia worldwide. these two pathogenic yeasts are distantly related, c. glabrata being part of the nakaseomyces, a group more closely related to saccharomyces cerevisiae. although c. glabrata was thought to be the only pathogenic nakaseomyces, two new pathogens have recently been described within this group: c. nivariensis and c. bracarensis. to gain insight into the genomic changes underlying the emergence o ... | 2013 | 24034898 |
| occurrence of killer candida glabrata clinical isolates. | in this work we characterized the occurrence of killer activity in 64 candida glabrata clinical isolates under different conditions. we found that only 6.25 % of the clinical isolates tested were positive for killer activity against a saccharomyces cerevisiae w303 sensitive strain. sensitivity of killer activity to different values of ph and temperatures was analyzed. we found that the killer activity presented by all isolates was resistant to every ph and temperature tested, although optimal ac ... | 2012 | 24031902 |
| epidemiology and phospholipase activity of oral candida spp. among patients with central nervous system diseases before and after dental cleaning procedure. | patients suffering of diseases that affect central nervous system may be considered more susceptible to the infectious diseases of mouth. sixty-nine patients suffering of cerebral palsy, down's syndrome and metal retardation were submitted to saliva examination for the presence of candida spp. before and after a procedure of dental cleaning. the isolates were submitted to assay for verifying phospholipase production. 55.10% of the patients provided isolation of candida spp. the frequency of isol ... | 2010 | 24031457 |
| candida glabrata esophagitis: new case reports and management. | candida esophagitis (ce) is a common opportunistic infection in the immunocompromised host. c. glabrata is rarely cited as agent of ce and has been underestimated due to lack of proper identification. in this study, two cases of c. glabrata esophagitis in aids and chagasic patients are reported. diagnosis of candida species should be considered an important key for the ideal choice of antifungal therapy against this mycosis. | 2008 | 24031216 |
| candidemia in intensive care unit patients: a one year study from a tertiary care center in south india. | the occurrence of candidemia is on a rise worldwide. non-albicans candida species have emerged as major causes of candidemia in many countries. added to it is the problem of antifungal resistance in candida isolates. | 2015 | 24029196 |
| photodynamic inactivation of clinical isolates of candida using photodithazine®. | this study evaluated the photodynamic inactivation (pdi) mediated by photodithazine(®) (pdz) against 15 clinical isolates of candida albicans, candida glabrata and candida tropicalis. each isolate, in planktonic and biofilm form, was exposed to pdi by assessing a range of pdz concentrations and light emitting diode fluences. cell survival of the planktonic suspensions was determined by colony forming units (cfu ml(-1)). the antifungal effects of pdi against biofilms were evaluated by cfu ml(-1) ... | 2013 | 24025068 |
| candidaemia in the non-neutropenic patient: a critique of the guidelines. | several guidelines have been published on the management of candidaemia. these guidelines vary in their recommendations, and the lack of consistency between the guidelines has implications for the management of candidaemia. we critiqued five guidelines, including the infectious diseases society of america (idsa) guidelines for the management of candidiasis, the canadian clinical practice guidelines for invasive candidiasis in adults, the joint recommendations of the german speaking mycological s ... | 2013 | 24016797 |
| pediatric risk factors for candidemia secondary to candida glabrata and candida krusei species. | this 13-year retrospective study investigated risk factors for candidemia secondary to candida species with increased likelihood of fluconazole resistance. of 344 candidemia cases, 23 were caused by c glabrata or c krusei (cgck). age >2 years, recent fluconazole exposure, and recent surgery were independent risk factors for cgck. | 2013 | 24009984 |
| invasive candidiasis in intensive care units in china: in vitro antifungal susceptibility in the china-scan study. | the objectives of this study were to determine species distribution and in vitro antifungal susceptibility of candida isolates identified in the multicentre china-scan study of invasive candida infection (ici) in intensive care units (icus) across china. | 2014 | 24004860 |
| isavuconazole pharmacodynamic target determination for candida species in an in vivo murine disseminated candidiasis model. | pharmacodynamic (pd) studies with triazoles in the neutropenic murine disseminated candidiasis model have been performed extensively for candida albicans. they have consistently shown that the pharmacodynamic index most closely correlated with efficacy is the ratio of the 24-h area under the concentration-time curve (auc) to the mic, and a target 24-h free-drug auc/mic ratio near 25 is associated with 50% of maximal microbiologic efficacy. we utilized this model to investigate the pharmacodynami ... | 2013 | 24002092 |
| comparison of nucleic acid amplification assays with bd affirm vpiii for diagnosis of vaginitis in symptomatic women. | a commercially available, nonamplified, nucleic acid probe-based test system (bd affirm vpiii) was compared with nucleic acid amplification (naa)-based assays for determining the etiology of vaginitis in a cohort of 323 symptomatic women. first, a semiquantitative, multiplexed pcr assay (bv-pcr) and the affirm vpiii gardnerellavaginalis test were compared with a unified bacterial-vaginosis (bv) reference standard incorporating both nugent gram stain scores and amsel clinical criteria. in the eva ... | 2013 | 23985917 |
| evaluation of bactec mycosis ic/f and plus aerobic/f blood culture bottles for detection of candida in the presence of antifungal agents. | clinical practice guidelines recommend performing follow-up cultures for patients with candidemia in order to determine the time when candida is cleared from the bloodstream. since this requires culturing blood samples from patients undergoing antifungal treatment, we evaluated two blood culture bottles (the bactec mycosis ic/f [micf], specifically adapted to the growth of fungi, and the bactec plus aerobic/f [paf], containing resins to inactivate anti-infective agents) for their effectiveness i ... | 2013 | 23985911 |
| flucytosine antagonism of azole activity versus candida glabrata: role of transcription factor pdr1 and multidrug transporter cdr1. | infections with the opportunistic yeast candida glabrata have increased dramatically in recent years. antifungal therapy of yeast infections commonly employs azoles, such as fluconazole (flc), but c. glabrata frequently develops resistance to these inhibitors of ergosterol biosynthesis. the pyrimidine analog flucytosine (5-fluorocytosine [5fc]) is highly active versus c. glabrata but is now rarely used clinically due to similar concerns over resistance and, a related concern, the toxicity associ ... | 2013 | 23979762 |
| cutaneous candidiasis caused by candida glabrata in a hiv/aids patient. | cutaneous candida infections may occur in patients with hiv/aids, cancer, receiving chemotherapy and solid organ transplantation. a 32-year-old woman was admitted to the department suffering from pruritic and erythematous plaque on left side of her face for the past two months. the patient was hiv positive, diagnosed five years previously, and had been on antiretroviral therapy (tenofovir/emtricitabine and lopinavir/ritonavir) for a year. she was not compliant with the medication. elevated hiv r ... | 2013 | 23970580 |
| determination of isavuconazole susceptibility of aspergillus and candida species by the eucast method. | isavuconazole is a novel expanded-spectrum triazole, which has recently been approved by the fda as an orphan drug to treat invasive aspergillosis and is currently being studied in phase iii clinical trials for invasive candidiasis. the susceptibility of relatively few clinical isolates has been reported. in this study, the isavuconazole susceptibilities of 1,237 aspergillus and 2,010 candida geographically diverse clinical isolates were determined by eucast methodology at four european mycology ... | 2013 | 23959309 |
| candida species contamination of preservation fluid-outcome of renal transplantation in 6 patients. | fungal infections are a rare but important cause of morbidity and mortality in kidney transplantation. fungal contamination of the kidney preservation fluid may, sometimes, be the cause of these infections. however, the clinical consequences of fungal contamination of this fluid are not completely understood and literature on this topic is controversial. the purpose of this study was to determine the incidence of preservation fluid contamination by fungi and its clinical consequences. | 2013 | 23953531 |
| breakthrough invasive fungal diseases during echinocandin treatment in high-risk hospitalized hematologic patients. | the frequency of breakthrough invasive fungal diseases (ifds) during echinocandin therapy is unclear. we retrospectively analyzed 534 hematologic patients treated with echinocandin (caspofungin, n = 55; micafungin, n = 306; anidulafungin, n = 173). four proven ifds were found, caused by candida parapsilosis (n = 2), c. parapsilosis and candida glabrata (n = 1), and fusarium species (n = 1). four cases of possible ifds were observed, all showing pulmonary infection. one case showed features sugge ... | 2014 | 23949318 |
| cytotoxicity of brazilian plant extracts against oral microorganisms of interest to dentistry. | with the emergence of strains resistant to conventional antibiotics, it is important to carry studies using alternative methods to control these microorganisms causing important infections, such as the use of products of plant origin that has demonstrated effective antimicrobial activity besides biocompatibility. therefore, this study aimed to evaluate the antimicrobial activity of plant extracts of equisetum arvense l., glycyrrhiza glabra l., punica granatum l. and stryphnodendron barbatimam ma ... | 2013 | 23945270 |
| mutation of the cgpdr16 gene attenuates azole tolerance and biofilm production in pathogenic candida glabrata. | the pdr16 gene encodes the homologue of sec14p, participating in protein secretion, regulation of lipid synthesis and turnover in vivo and acting as a phosphatidylinositol transfer protein in vitro. this gene is also involved in the regulation of multidrug resistance in saccharomyces cerevisiae and pathogenic yeasts. here we report the results of functional analysis of the cgpdr16 gene, whose mutation has been previously shown to enhance fluconazole sensitivity in candida glabrata mutant cells. ... | 2013 | 23939632 |
| antifungal cyclic peptides from the marine sponge microscleroderma herdmani. | screening natural product extracts from the national cancer institute open repository for antifungal discovery afforded hits for bioassay-guided fractionation. using lc-ms analysis to generate chemical structure information on potentially active compounds, two new cyclic hexapeptides, microsclerodermins j (1) and k (2), were isolated from the deep-water sponge microscleroderma herdmani, along with microsclerodermins a (3) and b (4), previously isolated from an unidentified microscleroderma speci ... | 2012 | 23936761 |
| a mouse model for candida glabrata hematogenous disseminated infection starting from the gut: evaluation of strains with different adhesion properties. | adhesion to digestive mucosa is considered a crucial first step in the pathogenicity of invasive candida infections. candida glabrata disseminated infections predominantly start from the gut. a mouse model of disseminated infection starting from the gut was set up. hematogenous dissemination was obtained after a low-protein diet followed by a regimen of cyclophosphamide-methotrexate and an oral inoculation of the yeasts via the drinking water. the liver was the first organ infected (day 7 post-i ... | 2013 | 23936069 |
| expression plasmids for use in candida glabrata. | we describe a series of cen/ars episomal plasmids containing different candida glabrata promoters, allowing for a range of constitutive or regulated expression of proteins in c. glabrata. the set of promoters includes three constitutive promoters (egd2pr, hht2pr, pdc1pr), two macrophage/phagocytosis-induced promoters (aco2pr, lys21pr), and one nutritionally regulated promoter (met3pr). each promoter was cloned into two plasmid backbones that differ in their selectable marker, ura3, or the domina ... | 2013 | 23934995 |
| possible transmission of candida albicans on an intensive care unit: genotype and temporal cluster analyses. | nosocomial transmission of candida spp. has not been fully explored and previous studies have shown conflicting results. | 2013 | 23927923 |
| clinical isolates and laboratory reference candida species and strains have varying abilities to form biofilms. | candida biofilms are a major virulence trait for this yeast. in this study, the biofilm-forming ability of the major medically important clinical and laboratory reference strains was compared. biofilms were quantified using traditional methods, that is, crystal violet (cv), tetrazolium (xtt) reduction and colony-forming unit assays (cfu), and two new methods: an automated cell counter (acc) and biofilm suspension turbidity (bst) method. biofilms could be categorized based on biofilm biomass (hig ... | 2013 | 23927631 |
| an in vitro study on the anti-adherence effect of brucea javanica and piper betle extracts towards oral candida. | the adherence of candida to mucosal surfaces is the initial step for successful invasive process of the oral cavity. the study aimed to investigate the effect of two plant extracts on the non-specific and specific bindings of oral candida. | 2013 | 23915676 |
| metal-based carboxamide-derived compounds endowed with antibacterial and antifungal activity. | a series of three bioactive thiourea (carboxamide) derivatives, n-(dipropylcarbamothioyl)-thiophene-2-carboxamide (l(1)), n-(dipropylcarbamothioyl)-5-methylthiophene-2-carboxamide (l(2)) and 5-bromo-n-(dipropylcarbamothioyl)furan-2-carboxamide (l(3)) and their cobalt(ii), copper(ii), nickel(ii) and zinc(ii) complexes (1)-(12) have been synthesized and characterized by their ir,(1)h-nmr spectroscopy, mass spectrometry and elemental analysis data. the crystal structure of one of the ligand, n-(dip ... | 2014 | 23914928 |
| susceptibility of multispecies biofilm to photodynamic therapy using photodithazine® | this in vitro study evaluated the effect of photodynamic therapy (pdt) on the multispecies biofilm of candida albicans, candida glabrata, and streptococcus mutans. standardized fungal and bacterial suspensions were cultivated appropriately for each species and inoculated in 96-well microtiter plates for mix-biofilm formation. after 48 h of incubation, the biofilms were submitted to pdt (p + l+) using photodithazine® (pdz) at 100, 150, 175, 200, or 250 mg/ml for 20 min and 37.5 j/cm(2) of light-e ... | 2013 | 23912779 |
| catalysis product captured in lumazine synthase from the fungal pathogen candida glabrata. | candida glabrata has emerged as an important fungal pathogen with intrinsic resistance to azole drugs. the limited efficacy of and resistance to existing antifungals is driving the need to identify new drug targets. the enzyme 6,7-dimethyl-8-(d-ribityl)lumazine synthase is part of the riboflavin-biosynthesis pathway essential to fungi and bacteria and is a potential drug target for the development of broad-spectrum antifungal drugs. the x-ray crystal structure of recombinant lumazine synthase fr ... | 2013 | 23897480 |
| the fate of linear dna in saccharomyces cerevisiae and candida glabrata: the role of homologous and non-homologous end joining. | in vivo assembly of plasmids has become an increasingly used process, as high throughput studies in molecular biology seek to examine gene function. in this study, we investigated the plasmid construction technique called gap repair cloning (grc) in two closely related species of yeast - saccharomyces cerevisiae and candida glabrata. grc utilizes homologous recombination (hr) activity to join a linear vector and a linear piece of dna that contains base pair homology. we demonstrate that a minimu ... | 2013 | 23894512 |
| synthesis and antifungal activities of miltefosine analogs. | miltefosine is an alkylphosphocholine that shows broad-spectrum in vitro antifungal activities and limited in vivo efficacy in mouse models of cryptococcosis. to further explore the potential of this class of compounds for the treatment of systemic mycoses, nine analogs (3a-3i) were synthesized by modifying the choline structural moiety and the alkyl chain length of miltefosine. in vitro testing of these compounds against the opportunistic fungal pathogens candida albicans, candida glabrata, can ... | 2013 | 23891181 |
| deficiencies in mitochondrial dna compromise the survival of yeast cells at critically high temperatures. | to address possible roles of mitochondrial genes in adaptation of eukaryotic cells to critical temperatures, we compared thermotolerance of mitochondrial rho mutants and wild type cells of six rho positive yeast species: candida glabrata, saccharomyces bayanus, saccharomyces cerevisiae, saccharomyces eubayanus, saccharomyces paradoxus and saccharomyces pastorianus. all rho mutants manifested compromised thermotolerance as a common phenotype. analysis of viabilities at critical temperatures (32-4 ... | 2014 | 23890722 |
| pyruvate production in candida glabrata: manipulation and optimization of physiological function. | candida glabrata, a multi-vitamin auxotrophic yeast, can accumulate a large amount of pyruvate extracellularly using glucose as the carbon source, a characteristic that has facilitated the cost-effective biotechnological production of pyruvate on an industrial scale. in this review, we describe the current advances in further improving the performance of c. glabrata for efficient pyruvate production, which includes: optimization of the vitamin and dissolved oxygen concentrations, regulation of i ... | 2016 | 23883073 |
| comparison of the vitek 2 yeast susceptibility system with clsi microdilution for antifungal susceptibility testing of fluconazole and voriconazole against candida spp., using new clinical breakpoints and epidemiological cutoff values. | a commercially available, fully automated yeast susceptibility test system (vitek 2; biomérieux, marcy d'etoile, france) was compared in 3 different laboratories with the clinical and laboratory standards institute (clsi) reference microdilution (bmd) method by testing 2 quality control strains, 10 reproducibility strains, and 425 isolates of candida spp. against fluconazole and voriconazole. reference clsi bmd mic endpoints and vitek 2 mic endpoints were read after 24 hours and 9.1-27.1 hours i ... | 2013 | 23870164 |
| species distribution and antifungal susceptibility of bloodstream fungal isolates in paediatric patients in mexico: a nationwide surveillance study. | to establish the species distribution and in vitro susceptibilities of 358 bloodstream fungal isolates from paediatric patients in mexico. | 2013 | 23869052 |
| rapid and reliable maldi-tof mass spectrometry identification of candida non-albicans isolates from bloodstream infections. | matrix-assisted laser desorption/ionization time-of-flight (maldi-tof) mass spectrometry (ms) fingerprinting has recently become an effective instrument for rapid microbiological diagnostics and in particular for identification of micro-organisms directly in a positive blood culture. the aim of the study was to evaluate a collection of 82 stored yeast isolates from bloodstream infection, by maldi-tof ms; 21 isolates were identified also directly from positive blood cultures and in the presence o ... | 2013 | 23845229 |
| usefulness of time-to-positivity in aerobic and anaerobic vials to predict the presence of candida glabrata in patients with candidaemia. | to determine whether time-to-positivity (ttp) in aerobic and anaerobic blood culture vials is useful to predict the presence of candida glabrata in patients with candidaemia. | 2013 | 23843300 |
| arteriovenous graft infection caused by candida glabrata: a case report and literature review. | the infection rate of arteriovenous (av) grafts is high, but fungal etiology is rare. only five cases of graft infection due to candida albicans (c. albicans) or c. tropicalis have been described in the literature. herein, we report the first case of av graft infection caused by c. glabrata. a 60-year-old woman on maintenance hemodialysis for end-stage renal disease was admitted because of intermittent fever, for 10 days. upon physical examination, tenderness over the av graft site was noticed. ... | 2016 | 23838263 |
| caspofungin benefit on phagocytes from patients with renal dysfunction infected with multidrug-resistant candida glabrata. | we evaluated the potential impact of caspofungin (cas) on the functional activities of polymorphonuclear leukocytes (pmns) from hemodialyzed patients (hds) and renal transplant recipients (rtrs) against a multidrug-resistant clinical strain of candida glabrata compared with those of pmns from healthy subjects (hss). | 2013 | 23834733 |
| new pcr test for detection of candida glabrata based on the molecular target chosen by the rapd technique. | rapid, reliable diagnosis is a necessary condition for the successful treatment of infections. such diagnostic assays are continually being developed. the paper presents a method for selecting the molecular target for pcr-based diagnostics based on the comparison of rapd patterns. a sequence encoding candida glabrata cbs138 hypothetical protein was selected. the limit of detection for pcr and real-time pcr reactions with dna extracted from blood samples spiked with candida glabrata was estimated ... | 2013 | 23829082 |
| clotrimazole-loaded eudragit® rs100 nanocapsules: preparation, characterization and in vitro evaluation of antifungal activity against candida species. | clotrimazole is a common choice for the treatment of vulvovaginal infections, but its low solubility and some side effects pose a challenge to its application. this work evaluated the feasibility to formulate clotrimazole-loaded cationic nanocapsules using eudragit® rs100 and medium chain triglycerides as polymer and oily core, respectively, by the method of interfacial deposition of a preformed polymer. the physicochemical characteristics of nanocapsule formulations were evaluated at 0day and 6 ... | 2012 | 23827586 |
| rapid emergence of echinocandin resistance in candida glabrata resulting in clinical and microbiologic failure. | we report a case of candida glabrata candidemia that developed resistance to micafungin within 8 days of initiation of therapy in a patient without previous echinocandin exposure or other known risk factors for clinical or microbiological failure. pre- and postresistant isolates were confirmed to be isogenic, and sequencing of hot spots known to confer echinocandin resistance revealed a phenylalanine deletion at codon 659 within fks2. | 2013 | 23817368 |
| microbial ethanol production in postmortem urine sample. | we present a case in which postmortem blood ethanol concentration was 0.02 g/kg and acetone concentration was 0.51 g/kg, while urine ethanol concentration was 6.0 g/kg and acetone concentration was 0.63 g/kg. in the urine sample, sodium fluoride was not added. the urinary ethanol concentration continued to increase without any remarkable increase of isopropanol concentration and external contamination was excluded. species of bacteria and yeasts, including candida glabrata, were isolated from ur ... | 2013 | 23812407 |
| yeasts isolated from nosocomial urinary infections: antifungal susceptibility and biofilm production. | urinary candida infections in the hospital environment are frequent and need to be better understood. | 2014 | 23810785 |
| the prevalence of oral candida infections in periodontitis patients with type 2 diabetes mellitus. | the purpose of this study was to determine the prevalence of candida spp. in periodontitis patients with type 2 diabetes mellitus. | 2013 | 23806705 |
| the dual role of candida glabrata drug:h+ antiporter cgaqr1 (orf cagl0j09944g) in antifungal drug and acetic acid resistance. | opportunistic candida species often have to cope with inhibitory concentrations of acetic acid, in the acidic environment of the vaginal mucosa. given that the ability of these yeast species to tolerate stress induced by weak acids and antifungal drugs appears to be a key factor in their persistence and virulence, it is crucial to understand the underlying mechanisms. in this study, the drug:h(+) antiporter cgaqr1 (orf cagl0j09944g), from candida glabrata, was identified as a determinant of resi ... | 2013 | 23805133 |
| the pneumocystis ace2 transcription factor regulates cell wall-remodeling genes and organism virulence. | pneumocystis carinii (pc) β-glucans are major components of the organism cell wall; yet, the regulation of pc cell wall genesis and remodeling is not well understood. ace2 transcription factors, which are present in many fungi, regulate glucanases and other enzymes needed for cell wall remodeling. the cloning and heterologous expression of pcace2 in ace2δ saccharomyces cerevisiae demonstrated that pcace2 can restore the defective glucanase and endochitinase gene expression of the mutant as well ... | 2013 | 23801335 |
| antifungal activity of the noncytotoxic human peptide hepcidin 20 against fluconazole-resistant candida glabrata in human vaginal fluid. | vaginal infections caused by candida glabrata are difficult to eradicate due to this species' scarce susceptibility to azoles. previous studies have shown that the human cationic peptide hepcidin 20 (hep-20) exerts fungicidal activity in sodium phosphate buffer against a panel of c. glabrata clinical isolates with different levels of susceptibility to fluconazole. in addition, the activity of the peptide was potentiated under acidic conditions, suggesting an application in the topical treatment ... | 2013 | 23796919 |
| antifungal activities of diphenyl diselenide alone and in combination with fluconazole or amphotericin b against candida glabrata. | here, we evaluated combinations of diphenyl diselenide [(phse)2] with fluconazole and amphotericin b in a checkerboard assay against clinical candida glabrata strains. minimal inhibitory concentration (geometric mean) ranged from 0.25 to >64 (5.16 μg/ml) for (phse)2, 1 to 32 (5.04 μg/ml) for fluconazole and 0.06 to 0.5 (0.18 μg/ml) for amphotericin b. synergistic (76.66 %) and indifferent (23.34 %) interactions were observed for (phse)2 + amphotericin b combination. (phse)2 + fluconazole combina ... | 2013 | 23793863 |
| identification of fungi species in the onychomycosis of institutionalized elderly. | superficial fungal infections are caused by dermatophytes, yeasts or filamentous fungi. they are correlated to the etiologic agent, the level of integrity of the host immune response, the site of the lesion and also the injured tissue. | 2017 | 23793195 |
| synthesis, structure and antifungal activity of thiophene-2,3-dicarboxaldehyde bis(thiosemicarbazone) and nickel(ii), copper(ii) and cadmium(ii) complexes: unsymmetrical coordination mode of nickel complex. | the reaction of nickel(ii), copper(ii) chlorides and cadmium(ii) chloride and bromide with thiophene-2,3-dicarboxaldehyde bis(thiosemicarbazone) (2,3btstch2) leads to a series of new complexes: [ni(2,3btstch)]cl, [cu(2,3btstc)], [cdcl2(2,3btstch2)] and [cdbr2(2,3btstch2)]. the crystal structures of the ligand and of [ni(2,3btstch)]cl complex have been determined. in this case, we remark an unusual non-symmetrical coordination mode for the two functional groups: one acting as a thione and the sec ... | 2013 | 23792913 |
| microbiological evaluation of bacterial and mycotic seal in implant systems with different implant-abutment interfaces and closing torque values. | the aim of this study was to evaluate the possible leakage of 3 species of bacteria (streptococcus sanguinis, fusobacterium nucleatum, and actinomyces odontolyticus) and of candida albicans and candida glabrata in osseointegrated implants with different implant-abutment interface (iai) geometry. | 2013 | 23782848 |
| antifungal activity of silver nanoparticles in combination with nystatin and chlorhexidine digluconate against candida albicans and candida glabrata biofilms. | although silver nanoparticles (sn) have been investigated as an alternative to conventional antifungal drugs in the control of candida-associated denture stomatitis, the antifungal activity of sn in combination with antifungal drugs against candida biofilms remains unknown. therefore, the aim of this study was to evaluate the antifungal efficacy of sn in combination with nystatin (nyt) or chlorhexidine digluconate (chg) against candida albicans and candida glabrata biofilms. the drugs alone or c ... | 2013 | 23773119 |
| [molecular biological approach to screening essential genes as potential targets for antifungal targets in pathogenic yeast candida]. | we established a system, named ets system, for screening and identification of essential genes from the pathogenic haploid yeast candida glabrata by using temperature-sensitive (ts) mutants. based on the general concepts that ts mutations are generated within essential genes in the genome by virtue of point mutation, the ets system enabled us to screen and identify a variety of essential genes from the c. glabrata genomic dna library as the genes that complement ts mutations. the ets system esta ... | 2013 | 23760075 |
| yeast dynamics during spontaneous fermentation of mawè and tchoukoutou, two traditional products from benin. | mawè and tchoukoutou are two traditional fermented foods largely consumed in benin, west africa. their preparations remain as a house art and they are the result of spontaneous fermentation processes. in this study, dynamics of the yeast populations occurring during spontaneous fermentations of mawè and tchoukoutou were investigated using both culture-dependent and -independent approaches. for each product, two productions were followed. samples were taken at different fermentation times and yea ... | 2013 | 23756236 |
| genital mycotic infections in patients with diabetes. | patients with diabetes, especially those with poorly controlled glycemia, are prone to developing genital mycotic infections-vulvovaginal candidiasis in women and candida balanitis in men-the latter almost exclusively in uncircumcised men. candida albicans is the most common pathogen causing balanitis and is also the dominant cause of vulvovaginal candidiasis in women with diabetes, although candida glabrata is a prominent pathogen in women with type 2 diabetes mellitus. candida glabrata is less ... | 2013 | 23748505 |
| [candidaemia by candida glabrata in a young patient with no previous symptoms]. | 2013 | 23746706 | |
| in vitro activities of isavuconazole and comparator antifungal agents tested against a global collection of opportunistic yeasts and molds. | isavuconazole is a new broad-spectrum triazole with a favorable pharmacokinetic and safety profile. we report the mic distributions for isavuconazole and 111 isolates of candida (42 candida albicans, 25 candida glabrata, 22 candida parapsilosis, 14 candida tropicalis, and 8 candida krusei isolates), as determined by clinical and laboratory standards institute (clsi) and european committee on antimicrobial susceptibility testing (eucast) broth microdilution (bmd) methods. also, the relative activ ... | 2013 | 23740727 |
| cell wall perturbation sensitizes fungi to the antimalarial drug chloroquine. | chloroquine (cq) has been a mainstay of antimalarial drug treatment for several decades. additional therapeutic actions of cq have been described, including some reports of fungal inhibition. here we investigated the action of cq in fungi, including the yeast model saccharomyces cerevisiae. a genomewide yeast deletion strain collection was screened against cq, revealing that bck1δ and slt2δ mutants of the cell wall integrity pathway are cq hypersensitive. this phenotype was rescued with sorbitol ... | 2013 | 23733464 |
| oral microflora and their relation to risk factors in hiv+ patients with oropharyngeal candidiasis. | the purpose of this study was to determine the prevalence of oral microflora and association of oral candidiasis and multiple risk factors in hiv(+) patients. | 2013 | 23721997 |
| echinocandin and triazole antifungal susceptibility profiles for clinical opportunistic yeast and mold isolates collected from 2010 to 2011: application of new clsi clinical breakpoints and epidemiological cutoff values for characterization of geographic and temporal trends of antifungal resistance. | the sentry antimicrobial surveillance program monitors global susceptibility and resistance rates of newer and established antifungal agents. we report the echinocandin and triazole antifungal susceptibility patterns for 3,418 contemporary clinical isolates of yeasts and molds. the isolates were obtained from 98 laboratories in 34 countries during 2010 and 2011. yeasts not presumptively identified by chromagar, the trehalose test, or growth at 42°c and all molds were sequence identified using in ... | 2013 | 23720791 |
| differential virulence of candida glabrata glycosylation mutants. | the fungus candida glabrata is an important and increasingly common pathogen of humans, particularly in immunocompromised hosts. despite this, little is known about the attributes that allow this organism to cause disease or its interaction with the host immune system. however, in common with other fungi, the cell wall of c. glabrata is the initial point of contact between the host and pathogen, and as such, it is likely to play an important role in mediating interactions and hence virulence. he ... | 2013 | 23720756 |
| metabolic engineering of torulopsis glabrata for malate production. | the yeast torulopsis glabrata cctcc m202019, which is used for industrial pyruvate production, was chosen to explore the suitability of engineering this multi-vitamin auxotrophic yeast for increased malate production. various metabolic engineering strategies were used to manipulate carbon flux from pyruvate to malate: (i) overexpression of pyruvate carboxylase and malate dehydrogenase; (ii) identification of the bottleneck in malate production by model inx804; (iii) simultaneous overexpression o ... | 2013 | 23707987 |
| prelog and anti-prelog stereoselectivity of two ketoreductases from candida glabrata. | two ketoreductases from candida glabrata were used for the asymmetric reduction of prochiral substituted acetophenones displayed different enantiopreference toward para-, meta-substituted and ortho-halogen substituted acetophenones with excellent enantioselectivity. homology modeling and docking analysis were in conformity with this interested enantiopreference obtained from experimental tests. the reduction of a series of other substituted aryl ketones was also investigated using the two ketore ... | 2013 | 23690040 |
| synthesis and biological evaluation of pyrazoline derivatives bearing an indole moiety as new antimicrobial agents. | 1-(p-methylphenyl)-3,5-diaryl-2-pyrazoline derivatives (2a-f) were synthesized via the treatment of 1-(1h-indol-3-yl)-3-aryl-2-propen-1-ones (1a-f) with p-methylphenylhydrazine hydrochloride in hot acetic acid. the structures of these compounds were elucidated by ir, ¹h nmr, and mass spectral data and elemental analysis. these compounds were investigated for their antimicrobial activity. brine-shrimp lethality assay was carried out to determine the toxicity of the compounds. compound 2e, which i ... | 2013 | 23681942 |
| candida orthopsilosis fungemias in a spanish tertiary care hospital: incidence, epidemiology and antifungal susceptibility. | few studies exist on prevalence of fungemia by candida orthopsilosis, with variable results. | 2014 | 23680638 |
| impact of imipenem treatment on colonic mycobiota in rats with double-hit sepsis. | broad-spectrum antibiotic administration promotes intestinal colonization of exogenous fungal pathogens in healthy animals and has been recognized as one of the risk factors of invasive fungal infection in clinical settings. it is unclear whether broad-spectrum antibiotic treatment would change the intestinal mycobiota without exogenous fungal challenge in the context of sepsis. | 2013 | 23673098 |
| small chromosomes among danish candida glabrata isolates originated through different mechanisms. | we analyzed 192 strains of the pathogenic yeast candida glabrata from patients, mainly suffering from systemic infection, at danish hospitals during 1985-1999. our analysis showed that these strains were closely related but exhibited large karyotype polymorphism. nine strains contained small chromosomes, which were smaller than 0.5 mb. regarding the year, patient and hospital, these c. glabrata strains had independent origin and the analyzed small chromosomes were structurally not related to eac ... | 2013 | 23670790 |
| caspofungin mics correlate with treatment outcomes among patients with candida glabrata invasive candidiasis and prior echinocandin exposure. | mutations in candida glabrata fks genes, which encode the echinocandin target enzyme, are independent risk factors for treatment failures during invasive candidiasis. we retrospectively compared the ability of caspofungin susceptibility testing methods to identify c. glabrata fks mutant isolates and predict outcomes among patients at our center. eight percent (10/120) of sterile-site c. glabrata isolates harbored fks1 (n = 3) or fks2 (n = 7) mutations, including 32% (10/32) recovered from patien ... | 2013 | 23669387 |
| local silencing controls the oxidative stress response and the multidrug resistance in candida glabrata. | in candida glabrata, the sirtuins sir2 and hst1 control the expression of a wide number of genes including adhesins required for host colonization and niacin transporters needed for growth. given that these sirtuins can be inactivated during infection, we asked if their inhibition could modify the response of c. glabrata to other stressful conditions. here, we found that deletion of hst1 decreases susceptibility of c. glabrata to fluconazole and hydrogen peroxide. the transcription factor pdr1 a ... | 2013 | 23651300 |
| investigation of the function of candida albicans als3 by heterologous expression in candida glabrata. | during hematogenously disseminated infection, blood-borne candida albicans invades the endothelial cell lining of the vasculature to invade the deep tissues. although the c. albicans als3 invasin is critical for invasion and damage of endothelial cells in vitro, a c. albicans als3δ/δ mutant has normal virulence in the mouse model of disseminated infection. we hypothesized that the contribution of als3 to virulence is obscured by the presence of additional c. albicans invasins. to elucidate the i ... | 2013 | 23630968 |
| candida glabrata drug:h+ antiporter cgqdr2 confers imidazole drug resistance, being activated by transcription factor cgpdr1. | the widespread emergence of antifungal drug resistance poses a severe clinical problem. though predicted to play a role in this phenomenon, the drug:h(+) antiporters (dha) of the major facilitator superfamily have largely escaped characterization in pathogenic yeasts. this work describes the first dha from the pathogenic yeast candida glabrata reported to be involved in antifungal drug resistance, the c. glabrata qdr2 (cgqdr2) gene (orf cagl0g08624g). the expression of cgqdr2 in c. glabrata was ... | 2013 | 23629708 |
| candida glabrata olecranon bursitis treated with bursectomy and intravenous caspofungin. | orthopedic surgeons are becoming more involved in the care of patients with septic arthritis and bursitis caused by yeast species. this case report involves a middle-aged immunocompromised female who developed a candida glabrata septic olecranon bursitis that developed after she received a corticosteroid injection in the olecranon bursa for presumed aseptic bursitis. candida (torulopsis) glabrata is the second most frequently isolated candida species from the bloodstream in the united states. in ... | 2013 | 23628576 |
| synthesis and carbonic anhydrase inhibitory properties of sulfamides structurally related to dopamine. | a series of novel sulfamides incorporating the dopamine scaffold were synthesized. reaction of amines and tert-butyl-alcohol/benzyl alcohol in the presence of chlorosulfonyl isocyanate (csi) afforded sulfamoyl carbamates, which were converted to the title compounds by treatment with trifluoroacetic acid or by palladium-catalyzed hydrogenolysis. inhibition of six α-carbonic anhydrases (cas, ec 4.2.1.1), that is, ca i, ca ii, ca va, ca ix, ca xii and ca xiv, and two β-cas from candida glabrata (cg ... | 2013 | 23623256 |
| histatin 5 resistance of candida glabrata can be reversed by insertion of candida albicans polyamine transporter-encoding genes dur3 and dur31. | candida albicans and candida glabrata are predominant fungi associated with oral candidiasis. histatin 5 (hst 5) is a small cationic human salivary peptide with high fungicidal activity against c. albicans, however many strains of c. glabrata are resistant. since hst 5 requires fungal binding to cell wall components prior to intracellular translocation, reduced hst 5 binding to c. glabrata may be the reason for its insensitivity. c. glabrata has higher surface levels of β-1,3-glucans as compared ... | 2013 | 23613860 |
| epidemiological changes with potential implication for antifungal prescription recommendations for fungaemia: data from a nationwide fungaemia surveillance programme. | significant changes in the management of fungaemia have occurred over the last decade with increased use of fluconazole prophylaxis, of empirical treatment and of echinocandins as first-line agents for documented disease. these changes may impact the epidemiology of fungaemia. we present nationwide data for denmark from 2010 to 2011. a total of 1081 isolates from 1047 episodes were recorded in 995 patients. the numbers of patients, episodes and recovered isolates increased by 13.1%, 14.5% and 14 ... | 2013 | 23607326 |
| expression of neutral β-glucosidase from scytalidium thermophilum in candida glabrata for ethanol production from alkaline-pretreated rice straw. | we successfully expressed the neutral β-glucosidase (bgl4) from scytalidium thermophilum in the thermotolerant yeast candida glabrata. compared to the strain expressing aspergillus acidic β-glucosidase (bgl1), the bgl4-expressing strain showed a higher cellobiose fermentation ability at ph 6.0 and 40°c, leading to a higher ethanol production from alkaline-pretreated rice straw. | 2013 | 23597919 |
| synthesis and antimicrobial activity of novel amphiphilic aromatic amino alcohols. | we report in this work the preparation and in vitro antimicrobial evaluation of novel amphiphilic aromatic amino alcohols synthesized by reductive amination of 4-alkyloxybenzaldehyde with 2-amino-2-hydroxymethyl-propane-1,3-diol. the antibacterial activity was determined against four standard strains (staphylococcus aureus, staphylococcus epidermidis, escherichia coli, pseudomonas aeruginosa) and 21 clinical isolates of methicillin-resistant staphylococcus aureus. the antifungal activity was eva ... | 2013 | 23587426 |
| a severe case of haemodynamic instability during anidulafungin administration. | anidulafugin is an echinocandin used for the treatment of candida infections in non-neutropenic adults. echinocandins show few drug-drug interactions and are usually well tolerated. we report a case of acute hypotension, bradycardia and haemodynamic instability with consecutive cardiopulmonary resuscitation during anidulafungin administration. | 2013 | 23550735 |
| caulerprenylols a and b, two rare antifungal prenylated para-xylenes from the green alga caulerpa racemosa. | two new prenylated para-xylenes, named caulerprenylols a (1) and b (2), were isolated from the green alga caulerpa racemosa, collected from the zhanjiang coastline, china. the structures of the two metabolites were elucidated on the basis of detailed spectroscopic analysis. this is the first report of prenylated para-xylenes from marine algae and from marine organisms as well. moreover, caulerprenylol b (2) is also characterized by an uncommon indane ring system. in in vitro bioassays, the new c ... | 2013 | 23548547 |
| the efficacy and safety of sertaconazole cream (2 %) in diaper dermatitis candidiasis. | diaper dermatitis (dd) is an inflammatory irritating condition that is common in infants. most cases are associated with the yeast colonization of candida or diaper dermatitis candidiasis (ddc), and therefore, the signs and symptoms improve with antimycotic treatment. sertaconazole is a broad-spectrum third-generation imidazole derivative that is effective and safe for the treatment for superficial mycoses, such as tineas, candidiasis, and pityriasis versicolor. our goal was to assess the effica ... | 2013 | 23546772 |
| detection and characterization of megasatellites in orthologous and nonorthologous genes of 21 fungal genomes. | megasatellites are large dna tandem repeats, originally described in candida glabrata, in protein-coding genes. most of the genes in which megasatellites are found are of unknown function. in this work, we extended the search for megasatellites to 20 additional completely sequenced fungal genomes and extracted 216 megasatellites in 203 out of 142,121 genes, corresponding to the most exhaustive description of such genetic elements available today. we show that half of the megasatellites detected ... | 2013 | 23543670 |
| species distribution and virulence factors of candida spp. isolated from the oral cavity of kidney transplant recipients in brazil. | although yeasts belonging to the genus candida are frequently seen as commensals in the oral cavity, they possess virulence attributes that contribute for pathogenicity. the aims of the present study were to study the prevalence of candida spp. isolated from the oral cavity of renal transplant recipients and to analyze strains virulence factors. we isolated a total of 70 candida strains from 111 transplant recipients, and candida albicans was the most prevalent species (82.86 %). oral candidiasi ... | 2013 | 23539354 |
| candida nivariensis as an etiologic agent of vulvovaginal candidiasis in a tertiary care hospital of new delhi, india. | candida nivariensis is a cryptic species, phenotypically indistinguishable from candida glabrata and identified by molecular methods. aside its isolation from broncho-alveolar lavage, we report for the first time the etiologic role of c. nivariensis in 4 patients with vulvovaginal candidiasis. of 100 phenotypically identified c. glabrata isolates originating from vaginal swabs, 4 were identified as c. nivariensis by polymerase chain reaction and confirmed by sequencing. all of the c. nivariensis ... | 2013 | 23537782 |
| candidemia among adults in soweto, south africa, 1990-2007. | studies on candidemia occurring among adults in southern african are limited. we aimed to document the epidemiology of candidemia among adults in soweto. | 2013 | 23535300 |
| expression of pneumocystis jirovecii major surface glycoprotein in saccharomyces cerevisiae. | the major surface glycoprotein (msg), which is the most abundant protein expressed on the cell surface of pneumocystis organisms, plays an important role in the attachment of this organism to epithelial cells and macrophages. in the present study, we expressed pneumocystis jirovecii msg in saccharomyces cerevisiae, a phylogenetically related organism. full-length p. jirovecii msg was expressed with a dna construct that used codons optimized for expression in yeast. unlike in pneumocystis organis ... | 2013 | 23532098 |
| an emerging issue of mixed yeast cultures. | different yeast species have different susceptibilities to commonly prescribed antifungal drugs. thus, it is important to accurately determine the species of pathogenic yeasts, especially when more than one species are in a specimen. | 2014 | 23523053 |
| [identification of three candida africana strains in senegal]. | the frequency of candidiasis has increased dramatically in recent years. candida albicans is the most common species. however, other species which are pathogenic and resistant to usual antifungal agents beginning to emerge. these include candida dubliniensis and candida africana, which share morphological similarities with candida albicans. thus, it is of interest to correctly identify the fungal isolates. | 2012 | 23518168 |
| antifungal activity, sar and physicochemical correlation of some thiazole-1,3,5-triazine derivatives. | present research communication was towards the investigation of antifungal minimum inhibitory concentration (mic) and minimum fingicidal concentration (mfc) activity of some substituted clubbed thiazole-1,3,5-triazines derivatives and effect of physicochemical properties on bioactivity. | 2012 | 23518015 |
| carbonic anhydrase inhibitors: inhibition of the β-class enzyme from the pathogenic yeast candida glabrata with sulfonamides, sulfamates and sulfamides. | the fungal pathogen candida glabrata encodes for a β-carbonic anhydrase (ca, ec 4.2.1.1), cgnce103, recently discovered. only anions have been investigated as cgnce103 inhibitors up until now. here we report the first sulfonamides inhibition study of this enzyme. simple sulfonamides showed weak or moderate cgnce103 inhibitory properties, whereas acetazolamide, and a series of 4-substituted ureido-benzene-sulfonamides, sulfamates and sulfamides showed effective cgnce103 inhibitory properties, wit ... | 2013 | 23511020 |
| the heme-binding protein dap1 links iron homeostasis to azole resistance via the p450 protein erg11 in candida glabrata. | the pathogenic fungus candida glabrata is relatively resistant to azole antifungals, which target lanosterol 14α-demethylase (erg11p) in the ergosterol biosynthesis pathway. our study revealed that c. glabrata exhibits increased azole susceptibility under low-iron conditions. to investigate the molecular basis of this phenomenon, we generated a strain lacking the heme (iron protoporphyrin ix)-binding protein dap1 in c. glabrata. the δdap1 mutant displayed growth defects under iron-limited condit ... | 2013 | 23496820 |
| [statement of the polish gynecological society expert group on the use of macmiror complex 500]. | the group of experts representing the polish gynecologic society has issued this statement based on the review of available literature on the potential benefits of the use of macmiror complex 500 in obstetrical and gynecologic practice. mixed vaginitis (mv) eg. the vaginal infection caused by at least two out of the triad of pathogens (fungi, bacteria and trichomonas vaginalis [tv]), constitutes the type of vaginitis which is underestimated as for its prevalence. mixed pathogens are responsible ... | 2012 | 23488303 |
| candida glabrata and fks mutations: witnessing the emergence of the true multidrug-resistant candida. | 2013 | 23487384 |