Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| nucleotide sequence of the clostridium thermocellum bgib gene encoding thermostable beta-glucosidase b: homology to fungal beta-glucosidases. | the nucleotide sequence of the bglb gene, coding for the thermostable beta-glucosidase b of clostridium thermocellum was determined. the coding region of 2265 bp was identified by comparison with the n-terminal amino acid sequence of beta-glucosidase b purified from escherichia coli. the derived amino acid sequence corresponding to a polypeptide of mr 84,100 was confirmed by sequencing of the c-terminal peptide generated by cleavage with cyanogen bromide. the protein bears no resemblance to othe ... | 1989 | 2505054 |
| lipid composition and lectin-mediated agglutination of candida kefyr cells grown in media enriched with choline or its analogues. | candida kefyr cells and their spheroplasts grown in media enriched with choline, n,n'-dimethylethanolamine (dmea) or ethanolamine (ea) showed decreased concanavalin a (con a) and ph to hemagglutinin (pha) mediated agglutination while supplementation with n-monomethylethanolamine (mmea) increased pha-mediated agglutination. in all cases, the amount of phospholipid was increased and, consequently, a decrease in the free sterol; phospholipid ratio was observed except in the case of mmea where this ... | 1989 | 2486007 |
| lectin mediated agglutination of candida kefyr cells and their spheroplasts grown in sterol enriched growth medium and its correlation with lipid composition. | supplementation of the growth medium with erosterol, cholesterol and lanosterol enriched the candida kefyr cells, presumably cell membranes with sterols. sterol enriched c. kefyr cells showed a decrease in percentage of pha and con-a mediated agglutination. sterol supplementation also increased the sterol: phospholipid ratio and in such cells unsaturated fatty acids predominated over saturated ones. the overall effect of these changes resulted in rigidifying the cell membranes as indicated by sh ... | 1988 | 2467528 |
| interaction of hexadecyltrimethylammonium bromide with yeast cells. | the interaction of hexadecyltrimethylammonium bromide (ctab) with two yeast cells, kluyveromyces fragilis and saccharomyces cerevisiae, has been studied. strong binding of ctab to the cell was inferred from 1h and 13c nmr studies, the probable site of binding being the cell-surface. 13c and 31p nmr studies have indicated facilitation of free passage of molecules from outside to inside the cell and vice versa on treatment with ctab. 31p nmr studies showed that intracellular ph (phi) was affected ... | 1990 | 2354849 |
| occurrence of the general control of amino acid biosynthesis in yeasts. | the response of three amino acid biosynthetic enzymes, threonine dehydratase, tyrosine aminotransferase and saccharopine dehydrogenase, to conditions of histidine, tryptophan or lysine limitation was investigated in 15 yeast species. the activities of all these enzymes increased about two- to fourfold as a result of action of the general control of amino acid biosynthesis in brettanomyces anomalus, candida maltosa, hansenula polymorpha, rhodosporidium toruloides, saccharomyces cerevisiae and yar ... | 1990 | 2338623 |
| modes of lactose uptake in the yeast species kluyveromyces marxianus. | twelve lactose-assimilating strains of the yeast species kluyveromyces marxianus and its varieties marxianus, lactis and bulgaricus were studied with respect to transport mechanisms for lactose, glucose and galactose, fermentation of these sugars and the occurrence of extracellular lactose hydrolysis. the strains fell into three groups. group i (two strains): fermentation of lactose, glucose and galactose, extracellular lactose hydrolysis, apparent facilitated diffusion of glucose and galactose; ... | 1990 | 2321931 |
| the yeasts of cheese brines. | a total of 365 yeasts were isolated from the brines of soft, semihard and hard cheeses from different manufacturers. identification was based on 131 characteristics, primarily employing a method with microtitration plates. most brines exhibited a characteristic yeast flora. the predominant strains proved to be mainly debaryomyces hansenii and candida versatilis. in a few brines trichosporon beigelii, c. rugosa, c. intermedia, kluyveromyces marxianus, saccharomyces sp. and c. tenuis/polymorpha we ... | 1990 | 2282287 |
| localization of inulinase and invertase in kluyveromyces species. | in vivo hydrolysis of inulin and sucrose was examined in selected yeasts of the genus kluyveromyces. cells, grown in sucrose-limited chemostat cultures, were subjected to treatments for the removal of inulinase, the enzyme responsible for the hydrolysis of both inulin and sucrose. the effects of these treatments were studied by measurement of inulin-dependent and sucrose-dependent oxygen consumption by cell suspensions. in kluyveromyces marxianus var. marxianus, inulinase was partially secreted ... | 1990 | 2268150 |
| cloning, sequencing and analysis of expression of a butyrivibrio fibrisolvens gene encoding a beta-glucosidase. | the cloning, expression and nucleotide sequence of a 3.74 kb dna segment on pls215 containing a beta-glucosidase gene (bgla) from butyrivibrio fibrisolvens h17c was investigated. the b. fibrisolvens bgla open reading frame (orf) of 2490 bp encoded a beta-glucosidase of 830 amino acid residues with a calculated mr of 91,800. in escherichia coli c600(pls215) cells the beta-glucosidase was localized in the cytoplasm and these cells produced an additional protein with an apparent mr of approximately ... | 1990 | 2262790 |
| discriminant analysis of cellular fatty acids of candida species, torulopsis glabrata, and cryptococcus neoformans determined by gas-liquid chromatography. | we used discriminant analysis of cellular fatty acid compositions determined by gas-liquid chromatography to differentiate yeastlike fungi (a total of 190 strains; including 37 candida albicans strains, 21 candida krusei strains, 13 candida guilliermondii strains, 37 candida tropicalis strains, 10 candida pseudotropicalis strains, 24 candida parapsilosis strains, 32 torulopsis glabrata strains, and 16 cryptococcus neoformans strains). previous results with a standard strain of c. albicans indica ... | 1990 | 2199515 |
| extraction, purification and characterization of adh1 from the budding yeast kluyveromyces marxianus. | the enzyme adh1 has been extracted and purified from the budding yeast kluyveromyces marxianus, and its enzymatic activity has been compared, with the adh1 extracted and purified in the same way from the well known yeast saccharomyces cerevisiae. k. marxianus adh1 has an optimal temperature higher than the s. cerevisiae enzyme (45-50 degrees vs 35 degrees c), a better stability to ph variations in the oxidative reaction (ph optimum 7.5), a lower michaelis constant for acetaldehyde, and a good ca ... | 1990 | 2193901 |
| human immunodeficiency virus causes mononuclear phagocyte dysfunction. | there is compelling clinical evidence for dysfunction of the mononuclear phagocyte system in patients with aids, which is believed due in part to loss of t-cell cooperativity. the direct consequences of human immunodeficiency virus infection on macrophage function are unknown. to address this question we infected normal human macrophages in vitro with a monocytotropic strain of human immunodeficiency virus and performed assays to quantify their extra- and intracellular killing ability. human imm ... | 1990 | 2187195 |
| effect of polymyxin b nonapeptide and polymyxin b sulphate on trichothecene mycotoxin sensitivity of yeasts using a conductimetric instrument. | the addition of polymyxin b sulphate (pbs), or an inactive by-product, polymyxin b nonapeptide (pbn) to a yeast bioassay system, increased its sensitivity to various toxic agents. the nil effect level (nel) of t-2 toxin was reduced from 0.1 to 0.01 microgram/ml for kluyveromyces fragilis gk 1005 in the presence of these agents when using a malthus at 192 conductimetric instrument. other synergistic agents (dmso, ethanol, cetyl trimethyl ammonium bromide and triton x-100) gave poor results in the ... | 1990 | 2168726 |
| continuous-culture study of the regulation of glucose and fructose transport in kluyveromyces marxianus cbs 6556. | regulation of transport of d-glucose and d-fructose was studied in kluyveromyces marxianus grown in continuous culture. both substrates could be transported by at least two different transport systems, low-affinity transport and high-affinity proton-sugar symport. the low-affinity transporter, specific for both glucose and fructose, was constitutively present and was apparently not regulated by carbon catabolite repression. regulation of the activity of the glucose- and fructose-specific proton ... | 1990 | 2160928 |
| [chemical composition of the cellular biomass of yeasts]. | this work is aimed at analyzing yeast strains, possibly used in animal feeding, obtained by batch cultivation from cheese whey as main carbohydrated substrate. for that purpose 10 yeast strains selected for its biomass production capacity were chemically analyzed. from the results, it can be observed that the chemical composition of the strains is quiet variable, showing in all cases high protein content, good solubility and enzymatic digestibility. in all of them, the rna content is low, being ... | 1990 | 2136518 |
| structure and properties of the extracellular inulinase of kluyveromyces marxianus cbs 6556. | in the yeast kluyveromyces marxianus two forms of inulinase were present, namely, an inulinase secreted into the culture fluid and an inulinase retained in the cell wall. both forms were purified and analyzed by denaturing and nondenaturing polyacrylamide gel electrophoresis. with the use of endo-beta-n-acetyl-glucosaminidase h, it was established that the enzyme retained in the cell wall and the enzyme secreted into the culture fluid have similar subunits consisting of a 64-kda polypeptide with ... | 1990 | 2135869 |
| chemical composition of a mixture of single-cell protein obtained from kluyveromyces fragilis and whey proteins. | a mixture of kluyveromyces fragilis biomass and coagulated whey proteins was obtained by fermentation of whole whey. this product had a chemical composition similar to that of washed products reported in the literature with high-crude protein and low-ash contents. the product had a high content of sulphur-containing amino acids and tryptophan, which are usually limiting in yeast biomass. lysine content was inexplicably lower than the expected value, being the limiting amino acid in this case. th ... | 1990 | 2133184 |
| effects of growth temperature on toxicity of t-2 toxin and roridin a to yeast. | in yeasts, growth temperature is known to affect the membrane phospholipid content. the effect of temperature on the growth inhibition of kluyveromyces marxianus and saccharomyces cerevisiae by the trichothecene mycotoxins, t-2 toxin and roridin a, was investigated. examination of ec50 values for t-2 toxin and roridin a showed that these toxins were least inhibitory to both yeasts at 30 and 25 degrees c, respectively. increasing or decreasing growth temperature from these temperatures gradually ... | 1991 | 2043279 |
| evolutionary relationships among pathogenic candida species and relatives. | small subunit rrna sequences have been determined for 10 of the most clinically important pathogenic species of the yeast genus candida (including torulopsis [candida] glabrata and yarrowia [candida] lipolytica) and for hansenula polymorpha. phylogenetic analyses of these sequences and those of saccharomyces cerevisiae, kluyveromyces marxianus var. lactis, and aspergillus fumigatus indicate that candida albicans, c. tropicalis, c. parapsilosis, and c. viswanathii form a subgroup within the genus ... | 1991 | 2007550 |
| antimicrobial activity of microgard against food spoilage and pathogenic microorganisms. | microgard, a commercially available fermented milk product containing antimicrobial metabolites, was a potent inhibitor for gram-negative bacteria such as pseudomonas, salmonella, and yersinia when 1% concentration was incorporated into agar media. gram-positive bacillus cereus, staphylococcus aureus, and listeria monocytogenes were insensitive to microgard. kluyveromyces marxianus, an unidentified black yeast, and penicillium expansum were partially suppressed, whereas aspergillus niger and a y ... | 1991 | 1906486 |
| synthesis and antifungal activity of some new benzimidazole derivatives. | synthesis and antifungal evaluation of 5-ethoxycarbonyl-2-(substituted-benzyl or phenoxymethyl)benzimidazoles are reported. structures of the compounds were elucidated with ir-, 1h-nmr-, 13c-nmr-, mass-spectra and elemental analysis. preliminary results show that none of the synthesized benzimidazole derivatives has antifungal activity at the concentration of 100 micrograms/ml against candida parapsilosis, candida stellatoidea, and candida pseudotropicalis. | 1991 | 1888265 |
| t-2 toxin degradation by micromycetes. | the biodegradation of t-2 toxin was studied by strains of micromycetes which were isolated from the environment. the 26 tested strains were divided into three groups. group contains strains which degraded t-2 toxin very fast. this toxin could not be chromatographically determined in the medium even after 48 hours of incubation and the antifungal activity of residua against kluyveromyces fragilis ccy-51-1-2 was low or zero. there were strains of alternaria sp., ulocladium sp., aspergillus candidu ... | 1991 | 1880407 |
| cloning and sequencing of the inulinase gene of kluyveromyces marxianus var. marxianus atcc 12424. | cell wall inulinase (ec 3.2.1.7) was purified from kluyveromyces marxianus var. marxianus (formerly k. fragilis) and its n-terminal 33-amino acid sequence was established. pcr amplification of cdna with 2 sets of degenerate primers yielded a genomic probe which was then used to screen a genomic library established in the yep351 yeast shuttle vector. one of the selected recombinant plasmids allowed an invertase-negative saccharomyces cerevisiae mutant to grow on inulin. it was shown to contain an ... | 1991 | 1840529 |
| ciclopirox nail lacquer 8%: in vivo penetration into and through nails and in vitro effect on pig skin. | this report presents original methods to assess the bioavailability of an antifungal drug from a varnish preparation in finger nails. for the studies with human volunteers a ciclopirox 8% nail lacquer was used to determine its efficacy in the treatment of onychomycoses. in vivo studies were performed on the fingernails of healthy volunteers by determining the total amount of ciclopirox penetrated per milligram of nail and the partition of the drug in the plate of the nails (technically divided i ... | 1991 | 1831626 |
| [biological value of the unicellular protein of kluyveromyces marxianus var. lactis]. | the ever increasing problem of environmental pollution and protein scarcity led us to begin producing protein biomass from cheese whey, having selected for this aim, kluyveromyces marxianus va. lactis strain, which represents the right relationship between its protein content (53.3% d.w.) and that of the rna (4.63% d.w.). on the other hand, the distribution of its essential amino acids is balanced, although it shows a deficiency in methionine, its value being 1.5 g/16 gn. the purpose of this wor ... | 1991 | 1822070 |
| cloning and disruption of the leu2 gene of kluyveromyces marxianus cbs 6556. | the leu2 gene, coding for beta-isopropylmalate dehydrogenase, of the yeast kluyveromyces marxianus was isolated and sequenced. an open reading frame, coding for a protein with a molecular weight of 38 kda was found. comparison of the deduced amino acid sequence of the leu2 gene with the corresponding enzymes of three other yeasts and two thermophilic bacteria, revealed extensive sequence similarities. the cloned gene could complement a leub mutation of escherichia coli and a leu2 mutation of sac ... | 1991 | 1803820 |
| transport of lactic acid in kluyveromyces marxianus: evidence for a monocarboxylate uniport. | lactic acid-grown cells of a strain of kluyveromyces marxianus transported d- and l-lactic acid by a saturable mechanism that was partially inducible and subject to glucose repression, with the following kinetic parameters at ph 5.4: vmax = 1.00 (+/- 0.13) mmol h-1 per g dry weight and ks = 0.42 (+/- 0.08) mm. lactic acid transport was competitively inhibited by pyruvic, glycolic, acetic and bromoacetic acids. the latter, a non-metabolizable analogue, was transiently accumulated, the extent depe ... | 1991 | 1789000 |
| temperature induced changes in microsomal (na+,k+)-atpase and lipid composition of candida kefyr. | growth temperature affected both the membrane lipid composition and microsoma (na+, k+)-atpase activity of candida kefyr. higher growth, temperature (37 degrees c) increased the amount of total lipids, phospholipids and free sterol. ratios of phosphatidylcholine to phosphatidylethanolamine as well saturated to unsaturated fatty acids increased with a rise in growth temperature. km of the atpase isolated from the yeast grown at 27 degrees c was minimum, suggesting that the membranes of c. kefyr g ... | 1991 | 1666728 |
| proton-motive force-driven d-galactose transport in plasma membrane vesicles from the yeast kluyveromyces marxianus. | galactose transport was studied in membrane vesicles, prepared by fusion of plasma membranes from the yeast kluyveromyces marxianus with proteoliposomes containing beef heart cytochrome c oxidase as a proton-motive force-generating system. sugar transport studies performed under nonenergized conditions revealed that, even at high protein to phospholipid ratios, not all vesicles contained a d-galactose-specific transporter. the amount of vesicles containing an active carrier proved to be proporti ... | 1991 | 1648083 |
| single cell protein production from beet pulp by mixed culture. | different mixed cultures of trichoderma reesei and a yeast were cultivated on beet pulp (bp)-containing medium. t. reesei and kluyveromyces marxianus offered a combination that gave high scp yields (51%) and efficiently converted bp into proteins (39.4%). the yeast extract in the basal medium could be substituted and the bp level multiplicated from 2 to 4% upon using mixed culture of t. reesei and k. marxianus. under these conditions the protein yields reached to a maximum value of 54% and highe ... | 1992 | 1605920 |
| effect of aeration rate on the alcoholic fermentation of whey by kluyveromyces fragilis. | in this paper, the influence of aeration rate on the alcoholic batch fermentation of whey by kluyveromyces fragilis nrrl y-2415 was investigated. assays in 1.5-l fermentor using concentrated whey permeate containing 100 g/l of lactose were carried out at different oxygen supply rate (klac*) from 0 to 82 mmol/lh. optimum response was obtained at 14 mmol/lh: ethanol production rate reached was 3.4 g/lh yielding 0.46 g of product per gram of initial lactose. an increase of klac* from 0 to 14 mmol/l ... | 1992 | 1605917 |
| distinct functional roles of two active site thiols in udpglucose 4-epimerase from kluyveromyces fragilis. | udpglucose 4-epimerase from kluyveromyces fragilis was earlier shown to have two conformationally vicinal thiols at the active site. upon treatment with diamide, these thiols form a disulfide linkage across the subunits that results in coordinated loss of catalytic activity and coenzyme fluorescence (ray, m., and bhaduri, a. (1980) j. biol. chem. 255, 10777-10786). employing a number of thiol-specific reagents, we now suggest discriminatory and nonidentical roles for these two thiols. kinetic an ... | 1992 | 1601848 |
| an essential histidine residue for the activity of udpglucose 4-epimerase from kluyveromyces fragilis. | udpglucose 4-epimerase from kluyveromyces fragilis was completely inactivated by diethylpyrocarbonate following pseudo-first order reaction kinetics. the ph profile of diethylpyrocarbonate inhibition and reversal of inhibition by hydroxylamine suggested specific modification of histidyl residues. statistical analysis of the residual enzyme activity and the extent of modification indicated modification of 1 essential histidine residue to be responsible for loss in catalytic activity of yeast epim ... | 1992 | 1601847 |
| effect of benzoic acid on metabolic fluxes in yeasts: a continuous-culture study on the regulation of respiration and alcoholic fermentation. | addition of benzoate to the medium reservoir of glucose-limited chemostat cultures of saccharomyces cerevisiae cbs 8066 growing at a dilution rate (d) of 0.10 h-1 resulted in a decrease in the biomass yield, and an increase in the specific oxygen uptake rate (qo2) from 2.5 to as high as 19.5 mmol g-1 h-1. above a critical concentration, the presence of benzoate led to alcoholic fermentation and a reduction in qo2 to 13 mmol g-1 h-1. the stimulatory effect of benzoate on respiration was dependent ... | 1992 | 1523884 |
| structure and expression of the abf1-regulated ribosomal protein s33 gene in kluyveromyces. | the abundant multifunctional protein abf1 of saccharomyces cerevisiae binds to the upstream region of several genes, including some ribosomal protein genes like the one encoding protein s33. deletion of the abf1-binding sequence lowers the transcription of these genes three- to more than ten-fold. we have isolated the s33 genes of two related yeast species, kluyveromyces lactis and kluyveromyces marxianus. comparison of the nucleotide sequences of these s33 genes with their counterpart from s. c ... | 1992 | 1481571 |
| structural and putative regulatory sequences of kluyveromyces ribosomal protein genes. | the transcription of the majority of the ribosomal protein (rp) genes of saccharomyces cerevisiae is activated by cis-acting elements, designated rpg boxes, which specifically bind the multifunctional protein rap1 in vitro. to investigate to what extent this global system of transcription regulation has been conserved, we have isolated a number of rp genes of the related yeast species kluyveromyces lactis and kluyveromyces marxianus, whose counterparts in saccharomyces are controlled by rap1. th ... | 1992 | 1481569 |
| growth of fermentative and non-fermentative yeasts in natural yoghurt, stored in polystyrene cartons. | permeation of oxygen through polystyrene packaging is a factor in the growth of yeasts in natural yoghurt. diffusion of oxygen through the packaging material can permit the growth of non-fermentative yeasts in yoghurt stored at refrigeration temperatures. yarrowia lipolytica, a non-fermentative yeasts which does not utilize lactose was isolated from yoghurt. the growth in natural yoghurt of yarrowia lipolytica and the lactose-fermenting yeast kluyveromyces marxianus was investigated. both yeasts ... | 1992 | 1419544 |
| synthesis of some hydroxamic acid derivatives of benzimidazole and their antibacterial and antifungal activities. | a number of 1h-benzimidazole-2-propanoic acid derivatives have been synthesized by phillips method, and their antibacterial and antifungal activities have been tested. the chemical structures of the synthesized compounds were elucidated by spectroscopic (ir, nmr, mass) and elementary analysis. investigation of antimicrobial activity of the compounds was done by agar dilution technique using bacteria (staphylococcus aureus atcc 25923, escherichia coli atcc 25922, pseudomonas aeruginosa atcc 27853 ... | 1992 | 1418079 |
| microenvironment at the substrate binding subsite of the active site of udpglucose 4-epimerase from kluyveromyces fragilis using a fluorescent analog of ump. | a chromophorics and fluorescent analog of uridine 5'-monophosphate (ump), a known competitive inhibitor of udpglucose 4-epimerase was synthesised. this analog, namely 2',3'-o-(2,4,6-trinitrocyclohexadienylidene) uridine 5'-monophosphate, was found to be a powerful reversible inhibitor of udpglucose 4-epimerase indicating its interaction with the substrate binding site of the enzyme. the extreme sensitivity of the fluorescence emission spectrum of this analog to solvent polarity makes it an excel ... | 1992 | 1398715 |
| a protein homologous to glyceraldehyde-3-phosphate dehydrogenase is induced in the cell wall of a flocculent kluyveromyces marxianus. | a protein with an apparent molecular weight of 37,000 (p37) is present in very large amounts in the cell wall of kluyveromyces marxianus, after the induction of flocculation of the yeast. this protein was isolated by preparative gel electrophoresis and its purity checked by sds-page and reverse-phase hplc. sds-page, endoglycosidase-h treatment and peptide sequencing indicated that p37 is a glycoprotein with a high identity to cytosolic glyceraldehyde-3-phosphate dehydrogenase from saccharomyces ... | 1992 | 1390912 |
| trichothecene synergism, additivity, and antagonism: the significance of the maximally quiescent ratio. | the interactive effect of the combinations of trichothecene mycotoxins often found in fungus infected plants, contaminated grain, and other biological systems is poorly understood. growth inhibition of the yeast kluyveromyces marxianus was used to measure the effects of ht-2 toxin, roridin a, and t-2 toxin as individual toxins or as binary mixtures. a value, the combination index, was derived which indicates the interactive effects of a binary mixture of toxins. the interaction is affected by th ... | 1992 | 1344899 |
| photodynamic treatment of yeast cells with the dye toluidine blue: all-or-none loss of plasma membrane barrier properties. | photodynamic treatment of kluyveromyces marxianus with the sensitizer toluidine blue leads to the loss of colony forming capacity. in this paper, the influence of this treatment on the barrier properties of the plasma membrane has been studied. photodynamic treatment with the dye toluidine blue resulted in efflux of potassium ions and e260-absorbing material. moreover, cells became stainable with erythrosine. it is concluded that the permeability change induced by photodynamic treatment proceeds ... | 1992 | 1322699 |
| [the role of candida kefyr (c. pseudotropicalis) in reproductive tract infections]. | frequency of appearance of c. kefyr strains, their diagnosis, susceptibility to drugs and importance in pathogenesis of reproductive tract mycoses were investigated. the investigated material consisted of 2717 strains, and there were 67 strains of c. kefyr isolated from vaginal mucous membrane. c. kefyr strains constituted 2.5% of total number of 2717 isolated strains and 8.2% within fungi other than c. albicans species. vaginal mycosis caused by c. kefyr occurs in presence of lactobacillus sp. ... | 1992 | 1297040 |
| synergistic action of amphotericin b and rifampin against candida species. | amphotericin b and rifampin act synergistically against certain yeasts in vitro. whether this synergism is a general phenomenon or whether the effect has strict species and strain requirements was studied. included in a survey of the genus candida were eight human isolates of candida albicans and one strain each of candida krusei, candida tropicalis, candida pseudotropicalis, candida parapsilosis, candida guilliermodnii, and candida stellatoidea. cultures in both control and drug-containing liqu ... | 1976 | 1245767 |
| selection of strain, growth conditions, and extraction procedures for optimum production of lactase from kluyveromyces fragilis. | forty-one strains of kluyveromyces fragilis (jörgensen) van der walt 1909 varied 60-fold in ability to produce lactase (beta-galactosidase). the four best strains were ucd no. 55-31 (northern regional research center nrrl y-1196), ucd no. c21(-), ucd no. 72-297(-), and ucd no. 55-61 (nrrl y-1109). biosynthesis of lactase during the growth of k. fragilis strain ucd no. 55-61 was followed on both lactose and sweet whey media. maximum enzyme yield was obtained at the beginning of the stationary pha ... | 1975 | 1194465 |
| the effects of various growth temperatures on nuclear division, dna and rna in the budding yeast, kluyveromyces fragilis. | 1975 | 1176961 | |
| 2'-deoxyadenosine and a23187 as agents for inducing synchrony in the budding yeast, kluyveromyces fragilis. | the effects of induction synchrony by 2'-deoxyadenosine and the ionophore a23187 were compared with selection synchrony methods in kluyveromyces fragilis. 2'-deoxyadenosine synchronizes nuclear division and this is followed by synchronous cell division, but dna synthesis is not synchronized. the ionophore a23187 also induces synchronous nuclear division and synchronous cell division, but likewise does not synchronize dna synthesis. | 1975 | 1100777 |
| [alcohol dehydrogenase (adh) in yeast cells. i. cytoplasmic, mitochondrial and nuclear adh in saccharomyces carlsbergensis and kluyveromyces fragilis]. | when grown in a medium containing lactat, saccharomyces carlsbergensis produces 5 times more ground-plasmatic adh than kluyveromyces fragilis. upon gelectrophoresis, k. fragilis exhibits 7 bands while s. carlsbergensis shows only one. in a polyacrylamid gradient, the adh's of both strains yield one band, the position of which corresponds to a molecular weight of about 160 000 d. in cell homogenates and mitochondrial fractions of s. carlsbergensis treated with ultrasound, an adh is detected which ... | 1976 | 983135 |
| accumulation of ergosterol during the cell cycle of the budding yeast kluyveromyces fragilis. | 1976 | 983132 | |
| a study on transformation in a mutant of candida pseudotropicalis. | this paper reports on the possible influence of various factors on the frequency of transformation in candida pseudtropicalis. the concentration of dna used varied between 25 mug and 100 mug/ml. the maximum percentage transformation 0-16% occured at 75 mug/ml. | 1976 | 959946 |
| ultrastructural changes, caused by the new antibiotic mucidin in candida pseudotropicalis. | 1976 | 947119 | |
| flow microcalorimetric investigation of yeast growth in a complex medium. | the growth of kluyveromyces fragilis in a complex medium under anaerobic and aerobic growth conditions has been conducted using a flow microcalorimeter. growth under defined conditions is characterized by an initial exponential rise in heat output rate to a maximum, followed by a decline to a baseline deflection. inadequate oxygenation can result in a more structured thermogram. the results are used to illustrate the deficiencies of some calorimetric incubations, and observations reported by oth ... | 1978 | 753949 |
| studies on the growth of kluyveromyces fragilis in whey for the production of yeast protein. | 1978 | 583484 | |
| evaluation of industrial yeasts for pathogenicity. | eleven yeasts representative of species of industrial interest were compared with candida albicans for their potential pathogenicity for untreated and cortisone-treated mice. only c. tropicalis produced a progressive infection similar to that produced by c. albicans. candida lipolytica, torulopsis spp., and hansenula polymorpha were not recovered from mice 6 days after inoculation. kluyveromyces fragilis, c. pseudotropicalis, c. utilis, c. guilliermondii and c. maltosa were recovered from mice b ... | 1979 | 441902 |
| environmentally-induced changes in the neutral lipids and intracellular vesicles of saccharomyces cerevisiae and kluyveromyces fragilis. | saccharomyces cerevisiae, grown aerobically or anaerobically under conditions which induce a requirement for a sterol and an unsaturated fatty acid, synthesized approximately the same amounts of neutral lipid and intracellular low-density vesicles, although the neutral lipids in aerobically-grown cells contained more esterified sterol and less triacylglycerol than those in anaerobically-grown cells. kluyveromyces fragilis synthesized much less neutral lipid and a smaller quantity of low-density ... | 1977 | 334099 |
| the immobilization of microbial cells, subcellular organelles, and enzymes in calcium alginate gels. | saccharomyces cerevisiae cells, kluyveromyces marxianus cells, inulase, glucose oxidase, chloroplasts, and mitochondria were immobilized in calcium alginate gels. ethanol production from glucose solutions by an immobilized preparation of s. cerevisiae was deomonstrated over a total of twenty-three days, and the half-life of such a preparation was shown to be about ten days. immobilized k. marxianus, inulase, and glucose oxidase preparations were used to demonstrate the porosity and retraining pr ... | 1977 | 321046 |
| two-step separation of human peripheral blood monocytes on discontinuous density gradients of colloidal silica-polyvinylpyrrolidinone. | normal human peripheral blood monocytes were purified by a two-step separation. the first step, the standard ficoll--hypaque (f--h) buoyant density centrifugation, yielded mainly mononuclear cells, of which 24 +/- 9% were monocytes. isopycnic centrifugation on discontinuous gradients of colloidal silica polyvinylpyrrolidinone (cs-pvp) further separated these mononuclear cells. the density interface between 1.070 and 1.060 g/ml yielded 82 +/- 7% monocytes, 5 +/- 4% granulocytes and 13 +/- 8% lymp ... | 1977 | 201698 |
| defective lung macrophages in pulmonary alveolar proteinosis. | lung macrophages obtained by segmental lavage from three patients with pulmonary alveolar proteinosis were studied in vitro. the macrophages exhibited morphologic abnormalities including excessive lipid accumulation and giant secondary lysosome formation. these cells survived poorly in tissue culture, showed impaired chemotactic activity, and had decreased adhesiveness to glass. they phagocytized normally but had substantially decreased capacity to kill ingested candida pseudotropicalis. evidenc ... | 1976 | 183578 |
| [the effect of dissolved oxygen tension on the energy metabolism of yeasts]. | the specific atp generation rate in yeasts was examined on the glycolytic pathway and on the respiratory chain as a function of the dissolved oxygen tension of the culture medium. two different strains were used: saccharomyces cerevisiae sensitive to the glucose effect and kluyveromyces fragilis insensitive to the catabolite respression when growing on lactose. the oxidative atp generation rate followed by these two strains a michaelis menten kinetics against the dissolved oxygen concentration. ... | 1977 | 146544 |
| defective blood mononuclear phagocyte function in patients with leprosy. | patients with lepromatous leprosy possess a defective lymphocyte function in vivo and in vitro that is less evident in the tuberculoid form. data concerning their macrophage ability to digest mycobacterium leprae are controversial. the purpose of this study was to determine whether monocytes from patients with either tuberculoid or lepromatous leprosy were altered in their enzyme systems, that is myeloperoxidase-dependent and myeloperoxidase-independent systems. the ability of adherent blood mon ... | 1979 | 122627 |
| characterisation and metabolic studies of saccharomyces cerevisiae and kluyveromyces fragilis by flow microcalorimetry. | the use of microcalorimetry in the routine identification of microorganisms is critically discussed and assessed. by use of flow microcalorimetric studies on saccharomyces cerevisiae and kluyveromyces fragilis the role of physical parameters and that of oxygen tension are discussed. the conclusion reached is that identification of microorganisms by microcalorimetry and subsequent discussion of metabolic events revealed by the thermogram, except under restrictive conditions, is inappropriate. how ... | 1979 | 45217 |
| histone-like protein fractions of kluyveromyces fragilis and their relation to the cell cycle. | protein fractions were obtained from kluyveromyces fragilis by ph titration, a technique used for histone extraction, following the inclusion of a distilled water extraction stage, and the fractions partially characterized. the inclusion of a distilled water step resulted in a tenfold purification of the fraction obtained at ph 2.20 as compared with ph titration alone. in synchronous cultures induced with 2'-deoxyadenosine or prepared by selection this fraction displayed a stepwise accumulation, ... | 1976 | 9743 |
| microorganisms and characteristics of laban. | laban had a titratable acidity of about 1.0%, a ph of 4.25, an ethanol content of 1.25%, and contained 4.2 mug acetaldehyde and 34 mug acetoin/ml. there was no diacetyl. five microorganisms, classified as streptococcus thermophilus, lactobacillus acidophilus, leuconostoc lactis, kluyveromyces fragilis, and saccharomyces cerevisiae, were responsible for the fermentation. streptococcus thermophilus and l. acidophilus were responsible for acid production with s. thermophilus producing acid more rap ... | 1976 | 2630 |