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application of a library of near isogenic lines to understand context dependent expression of qtl for grain yield and adaptive traits in bread wheat.previous quantitative trait loci (qtls) studies using the avalon × cadenza doubled haploid (dh) population identified eleven qtls determining plant height, heading date and grain yield. the objectives of this study were: (i) to provide insight into the effects of these qtls using reciprocal multiple near isogenic lines (nils) with each pair of alleles compared in both parental backgrounds (avalon or cadenza), (ii) quantifying epistasis by looking at the background effects and (iii) predict favou ...201627436187
mass spectrometric characterization of benzoxazinoid glycosides from rhizopus-elicited wheat (triticum aestivum) seedlings.benzoxazinoids function as defense compounds and have been suggested to possess health-promoting effects. in this work, the mass spectrometric behavior of benzoxazinoids from the classes benzoxazin-3-ones (with subclasses lactams, hydroxamic acids, and methyl derivatives) and benzoxazolinones was studied. wheat seeds were germinated with simultaneous elicitation by rhizopus. the seedling extract was screened for the presence of benzoxazinoid (glycosides) using reversed-phase ultra-high-performan ...201627431363
elucidation of the genetic basis of variation for stem strength characteristics in bread wheat by associative transcriptomics.the current approach to reducing the tendency for wheat grown under high fertilizer conditions to collapse (lodge) under the weight of its grain is based on reducing stem height via the introduction of rht genes. however, these reduce the yield of straw (itself an important commodity) and introduce other undesirable characteristics. identification of alternative height-control loci is therefore of key interest. in addition, the improvement of stem mechanical strength provides a further way throu ...201627423334
pm55, a developmental-stage and tissue-specific powdery mildew resistance gene introgressed from dasypyrum villosum into common wheat.powdery mildew resistance gene pm55 was physically mapped to chromosome arm 5vs fl 0.60-0.80 of dasypyrum villosum . pm55 is present in t5vs·5al and t5vs·5dl translocations, which should be valuable resources for wheat improvement. powdery mildew caused by blumeria graminis f. sp. tritici is a major wheat disease worldwide. exploiting novel genes effective against powdery mildew from wild relatives of wheat is a promising strategy for controlling this disease. to identify novel resistance genes ...201627422445
identification of novel abiotic stress proteins in triticum aestivum through functional annotation of hypothetical proteins.cereal grain bread wheat (t. aestivum) is an important source of food and belongs to poaceae family. hypothetical proteins (hps), i.e., proteins with unknown functions, share a substantial portion of wheat proteomes and play important roles in growth and physiology of plant system. several functional annotations studies utilizing the protein sequences for characterization of role of individual protein in physiology of plant systems were being reported in recent past. in this study, an integrated ...201627421996
detection of protein and dna damage induced by elevated carbon dioxide and ozone in triticum aestivum l. using biomarker and comet assay.this study was designed to compare the genetic effects of elevated carbon dioxide (co2) and ozone (o3), alone or in combination, under irrigated and non-irrigated conditions on proteins and dna of wheat (triticum aestivum l.). sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page), isozymes, random amplified polymorphic dna (rapd), and comet assays were used. sds-page analysis revealed distinctive polymorphisms (100%) based on the number of polypeptide bands (169) with molecular we ...201627420988
knock out of the phosphate 2 gene tapho2-a1 improves phosphorus uptake and grain yield under low phosphorus conditions in common wheat.mir399 and its target phosphate2 (pho2) play pivotal roles in phosphate signaling in plants. loss of function mutation in pho2 leads to excessive pi accumulation in shoots and growth retardation in diploid plants like arabidopsis thaliana and rice (oryza sativa). here we isolated three pho2 homologous genes tapho2-a1, -b1 and -d1 from hexaploid wheat (triticum aestivum). these tapho2 genes all contained mir399-binding sites and were able to be degraded by tae-mir399. tapho2-d1 was expressed much ...201627416927
evaluation of dough rheological properties and bread texture of pearl millet-wheat flour mix.this study was undertaken with the objective of formulating composite bread using pearl millet (pennisetum glaucum) and wheat (triticum aestivum) flours . rheological and bread making properties of composite flours were evaluated. mixolab results revealed torque increased and dough stability time decreased upon incorporation of pearl millet flour in wheat flour. the incorporation of millet flour at optimum level (5 %) led to an increase of the dough strength (w) and the elasticity-to-extensibili ...201627413235
development of methodologies for virus detection in soybean and wheat seeds.seeds that contain large amounts of oil, starch, fibers and phenols are the most difficult tissues for rna extraction. currently, there are some reports of virus detection in seeds using commercial kits for rna extraction. however, individual seeds were used, which may not be always suitable for analyses that deal with large amounts of seeds. sangha [1] described a simple, quick and efficient protocol for rna extraction and downstream applications in a group of seeds of jatropha (jatropha curcas ...201627408831
identification of winter-responsive proteins in bread wheat using proteomics analysis and virus-induced gene silencing (vigs).proteomic approaches were applied to identify protein spots involved in cold responses in wheat. by comparing the differentially accumulated proteins from two cultivars (uc1110 and pi 610750) and their derivatives, as well as the f10 recombinant inbred line population differing in cold-tolerance, a total of 20 common protein spots representing 16 unique proteins were successfully identified using 2-de method. of these, 14 spots had significantly enhanced abundance in the cold-sensitive parental ...201627402868
canopy temperature and vegetation indices from high-throughput phenotyping improve accuracy of pedigree and genomic selection for grain yield in wheat.genomic selection can be applied prior to phenotyping, enabling shorter breeding cycles and greater rates of genetic gain relative to phenotypic selection. traits measured using high-throughput phenotyping based on proximal or remote sensing could be useful for improving pedigree and genomic prediction model accuracies for traits not yet possible to phenotype directly. we tested if using aerial measurements of canopy temperature, and green and red normalized difference vegetation index as second ...201627402362
dissecting the u, m, s and c genomes of wild relatives of bread wheat (aegilops spp.) into chromosomes and exploring their synteny with wheat.goat grasses (aegilops spp.) contributed to the evolution of bread wheat and are important sources of genes and alleles for modern wheat improvement. however, their use in alien introgression breeding is hindered by poor knowledge of their genome structure and a lack of molecular tools. the analysis of large and complex genomes may be simplified by dissecting them into single chromosomes via flow cytometric sorting. in some species this is not possible due to similarities in relative dna content ...201627402341
genome-wide characterization of pectin methyl esterase genes reveals members differentially expressed in tolerant and susceptible wheats in response to fusarium graminearum.pectin methyl esterase (pme) genes code for enzymes that are involved in structural modifications of the plant cell wall during plant growth and development. they are also involved in plant-pathogen interaction. pme genes belong to a multigene family and in this study we report the first comprehensive analysis of the pme gene family in bread wheat (triticum aestivum l.). like in other species, the members of the tapme family are dispersed throughout the genome and their encoded products retain t ...201627393991
intraspecific polymorphisms of cytogenetic markers mapped on chromosomes of triticum polonicum l.triticum genus encloses several tetraploid species that are used as genetic stocks for expanding the genetic variability of wheat (triticum aestivum l.). although the t. aestivum (2n = 6x = 42, aabbdd) and t. durum (2n = 4x = 28, aabb) karyotypes were well examined by chromosome staining, giemsa c-banding and fish markers, other tetraploids are still poorly characterized. here, we established and compared the fluorescence in situ hybridization (fish) patterns on chromosomes of 20 accessions of t ...201627391447
occurrence and characterization of 2-hydroxy-1,4-benzoxazin-3-one and indole hydroxylases in juvenile wheat.cyclic hydroxamic acids, 2,4-dihydroxy-1,4-benzoxazin-3-one (diboa) and its 7-methoxy analogue (dimboa), occur transiently in high amounts in wheat and maize during the juvenile, non-autotrophic stage of growth. to elucidate the biosynthetic enzymes operating for the transient production of these compounds, we examined the hydroxylating activities for 2-hydroxy-1,4-benzoxazin-3-one (hboa), the immediate precursor of diboa, and indole, the first intermediate of the biosynthetic pathway that branc ...199927389648
predicting hybrid performances for quality traits through genomic-assisted approaches in central european wheat.bread-making quality traits are central targets for wheat breeding. the objectives of our study were to (1) examine the presence of major effect qtls for quality traits in a central european elite wheat population, (2) explore the optimal strategy for predicting the hybrid performance for wheat quality traits, and (3) investigate the effects of marker density and the composition and size of the training population on the accuracy of prediction of hybrid performance. in total 135 inbred lines of ...201627383841
decomposition dynamics and changes in chemical composition of wheat straw residue under anaerobic and aerobic conditions.soil aeration is a crucial factor that regulates crop residue decomposition, and the chemical composition of decomposing crop residues may change the forms and availability of soil nutrients, such as n and p. however, to date, differences in the chemical composition of crop straw residues after incorporation into soil and during its decomposition under anaerobic vs. aerobic conditions have not been well documented. the objective of the present study was to assess changes in the c-containing func ...201627380023
genetic association of stomatal traits and yield in wheat grown in low rainfall environments.in wheat, grain filling is closely related to flag leaf characteristics and function. stomata are specialized leaf epidermal cells which regulate photosynthetic co2 uptake and water loss by transpiration. understanding the mechanisms controlling stomatal size, and their opening under drought, is critical to reduce plant water loss and maintain a high photosynthetic rate which ultimately leads to elevated yield. we applied a leaf imprinting method for rapid and non-destructive phenotyping to expl ...201627378125
haplotypes of the tags5-a1 gene are associated with thousand-kernel weight in chinese bread wheat.in previous work, we cloned tags5 gene and found the association of tags5-a1 alleles with agronomic traits. in this study, the promoter sequence of the tags5-a1 gene was isolated from bread wheat. sequencing results revealed that a g insertion was found in position -1925 bp of the tags5-a1 gene (reference to atg), which occurred in the sp1 domain of the promoter sequence. combined with previous single nucleotide polymorphism (snp) in the tags5-a1 exon sequence, four genotypes were formed at the ...201627375643
the potential of hyperspectral patterns of winter wheat to detect changes in soil microbial community composition.reliable information on soil status and crop health is crucial for detecting and mitigating disasters like pollution or minimizing impact from soil-borne diseases. while infestation with an aggressive soil pathogen can be detected via reflected light spectra, it is unknown to what extent hyperspectral reflectance could be used to detect overall changes in soil biodiversity. we tested the hypotheses that spectra can be used to (1) separate plants growing with microbial communities from different ...201627375633
protective effects of id331 triticum monococcum gliadin on in vitro models of the intestinal epithelium.a growing interest in developing new strategies for preventing coeliac disease has motivated efforts to identify cereals with null or reduced toxicity. in the current study, we investigate the biological effects of id331 triticum monococcum gliadin-derived peptides in human caco-2 intestinal epithelial cells. triticum aestivum gliadin derived peptides were employed as a positive control. the effects on epithelial permeability, zonulin release, viability, and cytoskeleton reorganization were inve ...027374565
molecular characterization of the ige-binding epitopes in the fast ω-gliadins of triticeae in relation to wheat-dependent, exercise-induced anaphylaxis.fast ω-gliadins were minor components of wheat storage proteins but a major antigen triggering allergy to wheat. sixty-six novel full-length fast ω-gliadin genes with unique characteristics were cloned and sequenced from wheat and its relative species using a pcr-based strategy. their coding regions ranged from 177bp to 987bp in length and encoded 4.28kda to 37.56kda proteins. on the base of first three deduced amino acids at the n-terminal, these genes could be classified into the six subclasse ...201627374148
comprehensive characterization of c-glycosyl flavones in wheat (triticum aestivum l.) germ using uplc-pda-esi/hrmsn and mass defect filtering.a comprehensive characterization of c-glycosyl flavones in wheat germ has been conducted using multi-stage high resolution mass spectrometry (hrmsn ) in combination with a mass defect filtering (mdf) technique. mdf performed the initial search of raw data with defined c-glycosyl flavone mass windows and mass defect windows to generate the noise-reduced data focusing on targeted flavonoids. the high specificity of the exact mass measurement permits the unambiguous discrimination of acyl groups (n ...201627373213
field scale interaction and nutrient exchange between surface water and shallow groundwater in the baiyang lake region, north china plain.fertilizer input for agricultural food production, as well as the discharge of domestic and industrial water pollutants, increases pressures on locally scarce and vulnerable water resources in the north china plain. in order to: (a) understand pollutant exchange between surface water and groundwater, (b) quantify nutrient loadings, and (c) identify major nutrient removal pathways by using qualitative and quantitative methods, including the geochemical model phreeqc) a one-year study at a wheat ( ...201627372119
influence of speed and rainfall on large-scale wheat lodging from 2007 to 2014 in china.strong wind and heavy rain remain the two most important causes of large acreage wheat (triticum aestivum l.) lodging in china. for research the influence of wind speed and rainfall-separately as well as together-on the extent and degree of lodging, five levels of the severity of lodging were defined based on a combination of the lodging area and the degree of tilting. detailed meteorological information was studied on 52 instances of large-scale lodging that occurred from 2007 to 2014. the resu ...201627367926
stable isotope-assisted evaluation of different extraction solvents for untargeted metabolomics of plants.the evaluation of extraction protocols for untargeted metabolomics approaches is still difficult. we have applied a novel stable isotope-assisted workflow for untargeted lc-hrms-based plant metabolomics , which allows for the first time every detected feature to be considered for method evaluation. the efficiency and complementarity of commonly used extraction solvents, namely 1 + 3 (v/v) mixtures of water and selected organic solvents (methanol, acetonitrile or methanol/acetonitrile 1 + 1 (v/v) ...201627367667
the wheat r2r3-myb transcription factor tarim1 participates in resistance response against the pathogen rhizoctonia cerealis infection through regulating defense genes.the necrotrophic fungus rhizoctonia cerealis is a major pathogen of sharp eyespot that is a devastating disease of wheat (triticum aestivum). little is known about roles of myb genes in wheat defense response to r. cerealis. in this study, tarim1, a r. cerealis-induced wheat myb gene, was identified by transcriptome analysis, then cloned from resistant wheat ci12633, and its function and preliminary mechanism were studied. sequence analysis showed that tarim1 encodes a r2r3-myb transcription fac ...201627364458
back to the future - tapping into ancient grains for food diversity.einkorn, emmer, and spelt are old wheat species that have fed the world for centuries before they have nearly completely been replaced by modern bread wheat. nowadays, the diversity of these old species lies frozen in gene banks and rare attempts aim to exploit them as a source for genetic diversity in modern wheat breeding. here, we want to raise a debate on a more holistic exploitation of ancient species via their direct introduction to the consumer market as high quality products. although ex ...201627364231
cultivar mixture cropping increased water use efficiency in winter wheat under limited irrigation conditions.the effects of cultivar mixture cropping on yield, biomass, and water use efficiency (wue) in winter wheat (triticum aestivum l.) were investigated under non-irrigation (w0, no irrigation during growth stage), one time irrigation (w1, irrigation applied at stem elongation) and two times irrigation (w2, irrigation applied at stem elongation and anthesis) conditions. nearly 90% of cultivar mixture cropping treatments experienced an increase in grain yield as compared with the mean of the pure stan ...201627362563
genetic dissection of flag leaf morphology in wheat (triticum aestivum l.) under diverse water regimes.morphological traits related to flag leaves are determinant traits influencing plant architecture and yield potential in wheat (triticum aestivum l.). however, little is known regarding their genetic controls under drought stress. one hundred and twenty f8-derived recombinant inbred lines from a cross between two common wheat cultivars longjian 19 and q9086 were developed to identify quantitative trait loci (qtls) and to dissect the genetic bases underlying flag leaf width, length, area, length ...201627352616
qtl characterization of fusarium head blight resistance in cimmyt bread wheat line soru#1.fusarium head blight (fhb) resistant line soru#1 was hybridized with the german cultivar naxos to generate 131 recombinant inbred lines for qtl mapping. the population was phenotyped for fhb and associated traits in spray inoculated experiments in el batán (mexico), spawn inoculated experiments in ås (norway) and point inoculated experiments in nanjing (china), with two field trials at each location. genotyping was performed with the illumina iselect 90k snp wheat chip, along with a few ssr and ...201627351632
molecular genetic analysis of grain protein content and flour whiteness degree using rils in common wheat.grain protein content (gpc) and flour whiteness degree (fwd) are important qualitative traits in common wheat. quantitative trait locus (qtl) mapping for gpc and fwd was conducted using a set of 131 recombinant-inbred lines derived from the cross 'chuan 35050 × shannong 483' in six environmental conditions. a total of 22 putative qtls (nine gpc and 13 fwd) were identified on 12 chromosomes with individual qtl explaining 4.5-34.0% phenotypic variation. nine qtls (40.9%) were detected in two or mo ...201627350675
large-scale bioinformatic analysis of the regulation of the disease resistance nbs gene family by micrornas in poaceae.in the present study, we have screened 71, 713, 525, 119 and 241 mature mirna variants from hordeum vulgare, oryza sativa, brachypodium distachyon, triticum aestivum, and sorghum bicolor, respectively, and classified them with respect to their conservation status and expression levels. these poaceae non-redundant mirna species (1,669) were distributed over a total of 625 mir families, among which only 54 were conserved across two or more plant species, confirming the relatively recent evolutiona ...201727349470
identification and characterization of silicon efflux transporters in horsetail (equisetum arvense).silicon (si) is a beneficial element to plants, and its absorption via transporters leads to protective effects against biotic and abiotic stresses. in higher plants, two groups of root transporters for si have been identified: influx transporters (lsi1) and efflux transporters (lsi2). lsi1 transporters belong to the nipiii aquaporins, and functional lsi1s have been found in many plants species. much less is known about lsi2s that have been characterized in only a few species. horsetail (equiset ...201627344403
toxic effects of graphene on the growth and nutritional levels of wheat (triticum aestivum l.): short- and long-term exposure studies.increased use of graphene materials might lead to their release into the environment. however, only a few studies have investigated the impact of graphene-based materials on green plants. in the present study, effects of graphene on plant roots and shoots after 48h or 30days of hydroponic culture were evaluated to determine its phytotoxicity. results showed that although exposure to graphene (250, 500, 1000 and 1500mgl(-1)) significantly improved root elongation, root hair production was impaire ...201627343870
cerealsdb 3.0: expansion of resources and data integration.the increase in human populations around the world has put pressure on resources, and as a consequence food security has become an important challenge for the 21st century. wheat (triticum aestivum) is one of the most important crops in human and livestock diets, and the development of wheat varieties that produce higher yields, combined with increased resistance to pests and resilience to changes in climate, has meant that wheat breeding has become an important focus of scientific research. in ...201627342803
silencing of abcc13 transporter in wheat reveals its involvement in grain development, phytic acid accumulation and lateral root formation.low phytic acid is a trait desired in cereal crops and can be achieved by manipulating the genes involved either in its biosynthesis or its transport in the vacuoles. previously, we have demonstrated that the wheat taabcc13 protein is a functional transporter, primarily involved in heavy metal tolerance, and a probable candidate gene to achieve low phytate wheat. in the current study, rna silencing was used to knockdown the expression of taabcc13 in order to evaluate its functional importance in ...201627342224
evolution and distribution of hydrolytic enzyme activities during preharvest sprouting of wheat (triticum aestivum) in the field.to date, research on preharvest sprouted (phs) wheat has mostly been conducted on kernels germinated under laboratory conditions, which differ widely from conditions in the field. to obtain detailed knowledge of the evolution of hydrolytic enzyme activities in phs wheat (triticum aestivum), a broad collection of samples from three varieties was obtained by harvesting before, at, and after maturity. delaying harvest time coupled with periods of heavy rainfall caused sprouting in the kernels, obse ...201627341479
toxic effect of perfluorooctanoic acid (pfoa) on germination and seedling growth of wheat (triticum aestivum l.).as a persistent organic pollutant in the environment, perfluorooctanoic acid (pfoa) has been extensively investigated. it can accumulate in food chains and in the human body. this work investigated the effect of pfoa on wheat (triticum aestivum l.) germination and seedling growth by conducting a germination trial and a pot trial. a stimulatory effect of pfoa on seedling growth and root length of wheat was found at <0.2 mg kg(-1), while >800 mg kg(-1) pfoa inhibited germination rate, index, and r ...201627337433
microbial diversity of type i sourdoughs prepared and back-slopped with wholemeal and refined soft (triticum aestivum) wheat flours.the fermentation of type i sourdough was studied for 20 d with daily back-slopping under laboratory and artisan bakery conditions using 1 wholemeal and 2 refined soft wheat (triticum aestivum) flours. the sourdough bacterial and yeast diversity and dynamics were investigated by plate counting and a combination of culture-dependent and culture-independent pcr-dgge approach. the ph, total titrable acidity, and concentration of key organic acids (phytic, lactic, and acetic) were measured. three flo ...201627332783
cdna library enrichment of full length transcripts for smrt long read sequencing.the utility of genome assemblies does not only rely on the quality of the assembled genome sequence, but also on the quality of the gene annotations. the pacific biosciences iso-seq technology is a powerful support for accurate eukaryotic gene model annotation as it allows for direct readout of full-length cdna sequences without the need for noisy short read-based transcript assembly. we propose the implementation of the teloprime full length cdna amplification kit to the pacific biosciences iso ...201627327613
molecular characterization of three gibberellin-insensitive dwarf2 homologous genes in common wheat.f-box protein is a core component of the ubiquitin e3 ligase scf complex and is involved in the gibberellin (ga) signaling pathway. to elucidate the molecular mechanism of ga signaling in wheat, three homologous gibberellin-insensitive dwarf2 genes, tagid2s, were isolated from the chinese spring wheat variety. a subcellular localization assay in onion epidermal cells and arabidopsis mesophyll protoplasts showed that tagid2s are localized in the nuclei. the expression profiles using quantitative ...201627327160
engineering the 1bs chromosome arm in wheat to remove the rf (multi) locus restoring male fertility in cytoplasms of aegilops kotschyi, ae. uniaristata and ae. mutica.by removing the rf (multi) locus from chromosome 1bs of wheat via chromosome engineering we were able to generate a resource for the production of male sterile wheats in three new cytoplasms. cytoplasmic male sterility is an essential component in the development of many hybrid crops. in wheat (triticum aestivum l.) only the cytoplasm of t. timopheevi cytoplasm has been extensively tested even though many other cytoplasms are also known to produce male sterility. among them are the cytoplasms of ...201627325523
genetic and epigenetic alterations induced by different levels of rye genome integration in wheat recipient.the narrow genetic variation present in common wheat (triticum aestivum) varieties has greatly restricted the improvement of crop yield in modern breeding systems. alien addition lines have proven to be an effective means to broaden the genetic diversity of common wheat. wheat-rye addition lines, which are the direct bridge materials for wheat improvement, have been wildly used to produce new wheat cultivars carrying alien rye germplasm. in this study, we investigated the genetic and epigenetic ...201627323191
corrigendum morphological and sequence-related amplified polymorphism-based molecular diversity of local and exotic wheat genotypes - genet. mol. res. 15 (2): gmr.15027484.the correction is only in the name of the species studied and should be triticum aestivum, instead of triticum sativum.201627323047
the multifarious pgpr serratia marcescens cdp-13 augments induced systemic resistance and enhanced salinity tolerance of wheat (triticum aestivum l.).the present study demonstrates the plant growth promoting (pgp) potential of a bacterial isolate cdp-13 isolated from 'capparis decidua' plant, and its ability to protect plants from the deleterious effect of biotic and abiotic stressors. based on 16s rrna gene sequence analysis, the isolate was identified as serratia marcescens. among the pgp traits, the isolate was found to be positive for acc deaminase activity, phosphate solubilization, production of siderophore, indole acetic acid productio ...201627322827
gibberellins regulate the stem elongation rate without affecting the mature plant height of a quick development mutant of winter wheat (triticum aestivum l.).gibberellin (ga) is essential for determining plant height. alteration of ga content or ga signaling results in a dwarf or slender phenotype. here, we characterized a novel wheat mutant, quick development (qd), in which ga regulates stem elongation but does not affect mature plant height. qd and wild-type plants did not exhibit phenotypic differences at the seedling stage. from jointing to heading stage, qd plants were taller than wild-type plants due to elongated cells. however, wild-type and q ...201627317908
functional studies of heading date-related gene taprr73, a paralog of ppd1 in common wheat.photoperiod response-related genes play a crucial role in duration of the plant growth. in this study, we focused on taprr73, a paralog of "green revolution" gene ppd1 (taprr37). we found that overexpression of the truncated taprr73 form lacking part of the n-terminal pr domain in transgenic rice promoted heading under long day conditions. association analysis in common wheat verified that taprr73 was an important agronomic photoperiod response gene that significantly affected heading date and p ...201627313595
genomic prediction models for grain yield of spring bread wheat in diverse agro-ecological zones.genomic and pedigree predictions for grain yield and agronomic traits were carried out using high density molecular data on a set of 803 spring wheat lines that were evaluated in 5 sites characterized by several environmental co-variables. seven statistical models were tested using two random cross-validations schemes. two other prediction problems were studied, namely predicting the lines' performance at one site with another (pairwise-site) and at untested sites (leave-one-site-out). grain yie ...201627311707
development and validation of kasp assays for genes underpinning key economic traits in bread wheat.we developed and validated a robust marker toolkit for high-throughput and cost-effective screening of a large number of functional genes in wheat. functional markers (fms) are the most valuable markers for crop breeding programs, and high-throughput genotyping for fms could provide an excellent opportunity to effectively practice marker-assisted selection while breeding cultivars. here we developed and validated kompetitive allele-specific pcr (kasp) assays for genes that underpin economically ...201627306516
barley yellow dwarf virus infection and elevated co2 alter the antioxidants ascorbate and glutathione in wheat.plant antioxidants ascorbate and glutathione play an important role in regulating potentially harmful reactive oxygen species produced in response to virus infection. barley yellow dwarf virus is a widespread viral pathogen that systemically infects cereal crops including wheat, barley and oats. in addition, rising atmospheric co2 will alter plant growth and metabolism, including many potential but not well understood effects on plant-virus interactions. in order to better understand the wheat-b ...201627302010
diversification of the homoeologous lr34 sequences in polyploid wheat species and their diploid progenitors.allopolyploidization induces a multiple processes of genomic reorganization, including the structurally functional diversification of the homoeologous genes. an example of such diversification is the appearance of the lr34 gene on chromosome 7d of bread wheat t. aestivum (bad), the gene conferring durable, race non-specific protection against three fungal pathogens. in this study, we focused on the variability of a functionally critical region between exons 10-12 of lr34 among diploid progenitor ...201627300207
effect of recurrent selection on drought tolerance and related morpho-physiological traits in bread wheat.drought is one of the major abiotic stresses affecting wheat yield. a recurrent selection program was conducted to improve the drought tolerance and yield of bread wheat using drought tolerant advanced breeding lines from a drought tolerant x susceptible cross (hi 1500 x huw 510). the parental lines were evaluated for yield, biomass and harvest index (hi) in addition to the drought adaptive traits like canopy temperature (ct), chlorophyll content and normalized difference vegetative index (ndvi) ...201627300000
transcriptome profiling of the salt-stress response in triticum aestivum cv. kharchia local.kharchia local wheat variety is an indian salt tolerant land race known for its tolerance to salinity. however, there is a lack of detailed information regarding molecular mechanism imparting tolerance to high salinity in this bread wheat. in the present study, differential root transcriptome analysis identifying salt stress responsive gene networks and functional annotation under salt stress in kharchia local was performed. a total of 453,882 reads were obtained after quality filtering, using r ...201627293111
evolution of gene duplication in plants.ancient duplication events and a high rate of retention of extant pairs of duplicate genes have contributed to an abundance of duplicate genes in plant genomes. these duplicates have contributed to the evolution of novel functions, such as the production of floral structures, induction of disease resistance, and adaptation to stress. additionally, recent whole-genome duplications that have occurred in the lineages of several domesticated crop species, including wheat (triticum aestivum), cotton ...201627288366
the metabolite pathway between bundle sheath and mesophyll: quantification of plasmodesmata in leaves of c3 and c4 monocots.c4 photosynthesis is characterized by a co2-concentrating mechanism between mesophyll (m) and bundle sheath (bs) cells of leaves. this generates high metabolic fluxes between these cells, through interconnecting plasmodesmata (pd). quantification of these symplastic fluxes for modeling studies requires accurate quantification of pd, which has proven difficult using transmission electron microscopy. our new quantitative technique combines scanning electron microscopy and 3d immunolocalization in ...201627288224
the homologous identification of the stem rust resistance genes rdg5, adf3 and rga1 in the relatives of barley.the barley genes rpg5, rga1 and adf3, which provide a strong resistance to many pathotypes of stem rust, were cloned a few years ago, but it was still unclear whether their homologues were represented in wheat and in related species. the paper describes the results of a bioinformatic research to determine the homologues of rpg5, rga1 and adf3 in the genomes of triticum aestivum and several wild grasses, which breeders usually use as sources of stem rust resistance, and which are available in the ...201627281921
identification of qtls on chromosome 1b for grain quality traits in bread wheat (triticum aestivum l.).the present study was designed considering the importance of grain quality traits, genetic diversity and marker-trait association analysis in wheat. a significant amount of genetic diversity was found for various seed traits though the genotypes included in the study were found structured. the extent of polymorphism was high with a range of 2-13 alleles and average of 6.5 alleles per locus. population structure was detected with 30 unlinked ssrs that divided the population of 92 genotypes in thr ...201627281920
molecular and morpho-agronomical characterization of root architecture at seedling and reproductive stages for drought tolerance in wheat.water availability is a major limiting factor for wheat (triticum aestivum l.) production in rain-fed agricultural systems worldwide. root architecture is important for water and nutrition acquisition for all crops, including wheat. a set of 158 diverse wheat genotypes of australian (72) and indian (86) origin were studied for morpho-agronomical traits in field under irrigated and drought stress conditions during 2010-11 and 2011-12.out of these 31 indian wheat genotypes comprising 28 hexaploid ...201627280445
rhizotubes as a new tool for high throughput imaging of plant root development and architecture: test, comparison with pot grown plants and validation.in order to maintain high yields while saving water and preserving non-renewable resources and thus limiting the use of chemical fertilizer, it is crucial to select plants with more efficient root systems. this could be achieved through an optimization of both root architecture and root uptake ability and/or through the improvement of positive plant interactions with microorganisms in the rhizosphere. the development of devices suitable for high-throughput phenotyping of root structures remains ...201627279895
genotypic variation in spike fertility traits and ovary size as determinants of floret and grain survival rate in wheat.spike fertility traits are critical attributes for grain yield in wheat (triticum aestivum l.). here, we examine the genotypic variation in three important traits: maximum number of floret primordia, number of fertile florets, and number of grains. we determine their relationship in determining spike fertility in 30 genotypes grown under two contrasting conditions: field and greenhouse. the maximum number of floret primordia per spikelet (mfs), fertile florets per spikelet (ffs), and number of g ...201627279276
low molecular weight glutenin subunit gene glu-b3h confers superior dough strength and breadmaking quality in wheat (triticum aestivum l.).low molecular weight glutenin subunit is one of the important quality elements in wheat (triticum aestivum l.). although considerable allelic variation has been identified, the functional properties of individual alleles at glu-3 loci are less studied. in this work, we performed the first comprehensive study on the molecular characteristics and functional properties of the glu-b3h gene using the wheat cultivar cb037b and its glu-b3 deletion line cb037c. the results showed that the glu-b3h deleti ...201627273251
wheat (triticum aestivum l.)-based intercropping systems for biological pest control.wheat (triticum aestivum l.) is one of the most cultivated crops in temperate climates. as its pests are mainly controlled with insecticides that are harmful to the environment and human health, alternative practices such as intercropping have been studied for their potential to promote biological control. based on the published literature, this study aimed to review the effect of wheat-based intercropping systems on insect pests and their natural enemies. fifty original research papers were obt ...201627271821
efficient decolorization and detoxification of textile industry effluent by salvinia molesta in lagoon treatment.salvinia molesta, an aquatic fern was observed to have a potential of degrading azo dye rubine gfl up to 97% at a concentration of 100mg/l within 72h using 60±2g of root biomass. both root as well as stem tissues showed induction in activities of the enzymes such as lignin peroxidase, veratryl alcohol oxidase, laccase, tyrosinase, catalase, dcip reductase and superoxide dismutase during decolorization of rubine gfl. ftir, gc-ms, hplc and uv-visible spectrophotometric analysis confirmed phytotran ...201627268973
[pleiotropic effects of gibberellin-sensitive and gibberellin-insensitive dwarfing genes in common wheat of the southern step region of black sea].investigations of the pleiotropic effects of ga-sensitive (rht8) and ga-insensitive (rht-b1, rht-d1) dwarfing genes and a gene that determines the response of plants to photoperiod--ppd-d1 were carried out for three years in the southern step region of black sea bank on five different genetic backgrounds. it is shown that in addition to direct effects on plant height ga-sensitive and ga-insensitive dwarfing genes have pleiotropic effects on all studied traits except the number of fertile spikele ...201627266182
composition of cuticular waxes coating flag leaf blades and peduncles of triticum aestivum cv. bethlehem.the work herein presents comprehensive analyses of the cuticular wax mixtures covering the flag leaf blade and peduncle of bread wheat (triticum aestivum) cv. bethlehem. overall, gas chromatography-mass spectrometry and flame ionization detection revealed a wax coverage of flag leaf blades (16 μg/cm(2)) a third that of peduncles (49 μg/cm(2)). flag leaf blade wax was dominated by 1-alkanols, while peduncle wax contained primarily β-diketone and hydroxy-β-diketones, thus suggesting differential r ...201627264640
the stripe rust fungal effector pec6 suppresses pattern-triggered immunity in a host species-independent manner and interacts with adenosine kinases.we identified a wheat stripe rust (puccinia striiformis) effector candidate (pec6) with pattern-triggered immunity (pti) suppression function and its corresponding host target. pec6 compromised pti host species-independently. in nicotiana benthamiana, it hampers reactive oxygen species (ros) accumulation and callose deposition induced by pseudomonas fluorescens. in arabidopsis, plants expressing pec6 were more susceptible to pseudomonas syringae pv. tomato (pto) dc3000 δavrpto/δavrptob. in wheat ...201627252028
resistance of wheat accessions to the english grain aphid sitobion avenae.the english grain aphid, sitobion avenae, is a major pest species of wheat crops; however, certain varieties may have stronger resistance to infestation than others. here, we investigated 3 classical resistance mechanisms (antixenosis, antibiosis, and tolerance) by 14 wheat varieties/lines to s. avenae under laboratory and field conditions. under laboratory conditions, alatae given the choice between 2 wheat varieties, strongly discriminated against certain varieties. specifically, the 'amigo' v ...201627249206
an intercalary translocation from agropyron cristatum 6p chromosome into common wheat confers enhanced kernel number per spike.this study explored 6p chromosomal translocations in wheat, and determined the effects of 6p intercalary chromosome segments on kernel number per wheat spike. exploiting and utilising gene(s) from wild relative species has become an essential strategy for wheat crop improvement. in the translocation line pubing2978, the intercalary 6p chromosome segment from agropyron cristatum (l.) gaertn. (2n = 4x = 28, pppp) carried valuable multi-kernel gene(s) and was selected from the offspring of the comm ...201627246315
introgression of chromosome segments from multiple alien species in wheat breeding lines with wheat streak mosaic virus resistance.pyramiding of alien-derived wheat streak mosaic virus (wsmv) resistance and resistance enhancing genes in wheat is a cost-effective and environmentally safe strategy for disease control. pcr-based markers and cytogenetic analysis with genomic in situ hybridisation were applied to identify alien chromatin in four genetically diverse populations of wheat (triticum aestivum) lines incorporating chromosome segments from thinopyrum intermedium and secale cereale (rye). out of 20 experimental lines, 1 ...201627245423
flour quality and related molecular characterization of high molecular weight glutenin subunit genes from wild emmer wheat accession td-256.to clarify the effect of high molecular weight glutenin subunit (hmw-gs) from wild emmer wheat on flour quality, which has the same mobility as that from common wheat, the composition and molecular characterization of hmw-gs from wild emmer wheat accession td-256, as well as its flour quality, were intensively analyzed. it is found that the mobilities of glu-a1 and glu-b1 subunits from td-256 are consistent with those of bread wheat cv. 'xiaoyan 6'. nevertheless, dough rheological properties of ...201627243935
soil-borne wheat mosaic virus infectious clone and manipulation for gene-carrying capacity.a full-length infectious cdna clone of soil-borne wheat mosaic virus (sbwmv; genus furovirus; family virgaviridae) was developed for agrobacterium delivery. the cloned virus can be agroinfiltrated to nicotiana benthamiana for subsequent infection of wheat (triticum aestivum, l.). the utility of the virus as a vector for gene silencing and expression was assessed through sequence insertions in multiple sites of rna2. virus-induced photobleaching was observed in n. benthamiana but not in wheat, de ...201627236459
comprehensive analyses of the annexin gene family in wheat.annexins are an evolutionarily conserved multigene family of calcium-dependent phospholipid binding proteins that play important roles in stress resistance and plant development. they have been relatively well characterized in model plants arabidopsis (arabidopsis thaliana) and rice (oryza sativa), but nothing has been reported in hexaploid bread wheat (triticum aestivum) and barely (hordeum vulgare), which are the two most economically important plants.201627236332
nighttime evaporative demand induces plasticity in leaf and root hydraulic traits.increasing evidence suggests that nocturnal transpiration rate (trn ) is a non-negligible contributor to global water cycles. short-term variation in nocturnal vapor pressure deficit (vpdn ) has been suggested to be a key environmental variable influencing trn . however, the long-term effects of vpdn on plant growth and development remain unknown, despite recent evidence documenting long-term effects of daytime vpd on plant anatomy, growth and productivity. here we hypothesized that plant anatom ...201627235372
tamca1, a regulator of cell death, is important for the interaction between wheat and puccinia striiformis.metacaspase orthologs are conserved in fungi, protozoa and plants, however, their roles in plant disease resistance are largely unknown. in this study, we identified a triticum aestivum metacaspase gene, tamca1, with three copies located on chromosomes 1a, 1b and 1d. the tamca1 protein contained typical structural features of type i metacaspases domains, including an n-terminal pro-domain. transient expression analyses indicated that tamca1 was localized in cytosol and mitochondria. tamca1 exhib ...201627230563
overexpression of wheat gene tamor improves root system architecture and grain yield in oryza sativa.improved root architecture is an effective strategy to increase crop yield. we demonstrate that overexpression of transcription factor gene more root (tamor) from wheat (triticum aestivum l.) results in more roots and higher grain yield in rice (oryza sativa). tamor, encoding a plant-specific transcription factor belonging to the asymmetric leaves2/lateral organ boundaries (as2/lob) protein family, is highly conserved in wheat and its wild relatives. in this study, tissue expression patterns ind ...201627229732
rapid evolutionary dynamics in a 2.8-mb chromosomal region containing multiple prolamin and resistance gene families in aegilops tauschii.prolamin and resistance gene families are important in wheat food use and in defense against pathogen attacks, respectively. to better understand the evolution of these multi-gene families, the dna sequence of a 2.8-mb genomic region, representing an 8.8 cm genetic interval and harboring multiple prolamin and resistance-like gene families, was analyzed in the diploid grass aegilops tauschii, the d-genome donor of bread wheat. comparison with orthologous regions from rice, brachypodium, and sorgh ...201627228577
foxtail mosaic virus-induced gene silencing in monocot plants.virus-induced gene silencing (vigs) is a powerful technique to study gene function in plants. however, very few vigs vectors are available for monocot plants. here we report that foxtail mosaic virus (fomv) can be engineered as an effective vigs system to induce efficient silencing of endogenous genes in monocot plants including barley (hordeum vulgare l.), wheat (triticum aestivum) and foxtail millet (setaria italica). this is evidenced by fomv-based silencing of phytoene desaturase (pds) and m ...201627225900
soil water balance and water use efficiency of dryland wheat in different precipitation years in response to green manure approach.winter wheat (triticum aestivum l.) monoculture is conventionally cultivated followed by two to three months of summer fallow in the loess plateau. to develop a sustainable cropping system, we conducted a six-year field experiment to investigate the effect of leguminous green manure (lgm) instead of bare fallow on the yield and water use efficiency (wue) of winter wheat and the soil water balance (swb) in different precipitation years in a semi-arid region of northwest china. results confirmed t ...201627225842
a metabolic gene cluster in the wheat w1 and the barley cer-cqu loci determines β-diketone biosynthesis and glaucousness.the glaucous appearance of wheat (triticum aestivum) and barley (hordeum vulgare) plants, that is the light bluish-gray look of flag leaf, stem, and spike surfaces, results from deposition of cuticular β-diketone wax on their surfaces; this phenotype is associated with high yield, especially under drought conditions. despite extensive genetic and biochemical characterization, the molecular genetic basis underlying the biosynthesis of β-diketones remains unclear. here, we discovered that the whea ...201627225753
genetic control of protein content and sedimentation volume in european winter wheat cultivars.breeding of bread wheat in the last decades has maintained a high baking quality despite the intensive selection for grain yield. the quality trait sedimentation volume but not protein content is mainly controlled by the glu - a1, glu - b1, glu - d1, gli - b1 , and pinb - d1 loci which are differentially used in varieties from different european origins. protein content and sedimentation volume are two important quality traits in wheat breeding. in this study, we used a panel of 407 european win ...201627225454
drought-responsive wrky transcription factor genes tawrky1 and tawrky33 from wheat confer drought and/or heat resistance in arabidopsis.drought stress is one of the major causes of crop loss. wrky transcription factors, as one of the largest transcription factor families, play important roles in regulation of many plant processes, including drought stress response. however, far less information is available on drought-responsive wrky genes in wheat (triticum aestivum l.), one of the three staple food crops.201627215938
effect of plant growth promoting bacteria associated with halophytic weed (psoralea corylifolia l) on germination and seedling growth of wheat under saline conditions.halotolerant bacteria associated with psoralea corylifolia l., a luxuriantly growing annual weed in salinity-affected semi-arid regions of western maharashtra, india were evaluated for their plant growth-promoting activity in wheat. a total of 79 bacteria associated with different parts viz., root, shoot and nodule endophytes, rhizosphere, rhizoplane, and leaf epiphytes, were isolated and grouped based on their habitat. twelve bacteria isolated for their potential in plant growth promotion were ...201627215915
[genealogical analysis of the use of two wheatgrass (agropyron) species in common wheat (triticum aestivum l.) breeding for disease resistance].during the last 80 years, in order to increase the genetic variability of wheat, translocations containing nine elongated wheatgrass (agropyron elongatum) and eight intermediate wheatgrass (agropyron intermedium) genes, which control resistance to pathogens, were transferred to this crop culture. genealogical and statistical analysis of 1500 varieties developed using the wheatgrass gave evidence of the continuing increase in the proportion of such varieties in the total number of wheat varieties ...201627215032
[prevalence of vrn1 locus alleles among spring common wheat cultivars cultivated in western siberia].with the use of allele-specific primers developed for the vrn1 loci, the allelic diversity of the vrn-a1, vrn-b1, and vrn-d1 genes was studied in 148 spring common wheat cultivars cultivated under the conditions of western siberia. it was demonstrated that modern western siberian cultivars have the vrn-a1a allele, which is widely distributed in the world (alone or in combination with the vrn-b1a and vrn-b1c alleles). it was established that the main contribution in acceleration of the.seedling-h ...201627215031
inheritance and molecular mapping of an all-stage stripe rust resistance gene derived from the chinese common wheat landrace "yilongtuomai".yellow or stripe rust, caused by puccinia striiformis f. sp. tritici (pst), is a devastating foliar disease that affects common wheat (triticum aestivum l.) around the world. in china, common wheat landraces are potential sources of disease and abiotic stress resistance genes for wheat improvement. yilongtuomai (yl), a wheat landrace from yilong county, sichuan province, shows high levels of resistance against most chinese pst races. in this study, the resistance of yl to stripe rust disease was ...201627208148
dissecting mirnas in wheat d genome progenitor, aegilops tauschii.as the post-transcriptional regulators of gene expression, micrornas or mirnas comprise an integral part of understanding how genomes function. although mirnas have been a major focus of recent efforts, mirna research is still in its infancy in most plant species. aegilops tauschii, the d genome progenitor of bread wheat, is a wild diploid grass exhibiting remarkable population diversity. due to the direct ancestry and the diverse gene pool, a. tauschii is a promising source for bread wheat impr ...201627200073
characterization of a new pm2 allele conferring powdery mildew resistance in the wheat germplasm line fg-1.powdery mildew has a negative impact on wheat production. novel host resistance increases the diversity of resistance genes and helps to control the disease. in this study, wheat line fg-1 imported from france showed a high level of powdery mildew resistance at both the seedling and adult stages. an f2 population and f2:3 families from the cross fg-1 × mingxian 169 both fit mendelian ratios for a single dominant resistance gene when tested against multiple avirulent blumeria tritici f. sp. triti ...201627200022
host specialization of the blast fungus magnaporthe oryzae is associated with dynamic gain and loss of genes linked to transposable elements.magnaporthe oryzae (anamorph pyricularia oryzae) is the causal agent of blast disease of poaceae crops and their wild relatives. to understand the genetic mechanisms that drive host specialization of m. oryzae, we carried out whole genome resequencing of four m. oryzae isolates from rice (oryza sativa), one from foxtail millet (setaria italica), three from wild foxtail millet s. viridis, and one isolate each from finger millet (eleusine coracana), wheat (triticum aestivum) and oat (avena sativa) ...201627194050
genome-wide association mapping of anther extrusion in hexaploid spring wheat.in a number of crop species hybrids are able to outperform line varieties. the anthers of the autogamous bread wheat plant are normally extruded post anthesis, a trait which is unfavourable for the production of f1 hybrid grain. higher anther extrusion (ae) promotes cross fertilization for more efficient hybrid seed production. therefore, this study aimed at the genetic dissection of ae by genome wide association studies (gwas) and determination of the main effect qtl. we applied gwas approach t ...201627191600
mutagenicity, cytotoxicity and phytotoxicity evaluation of biodegraded textile effluent by fungal ligninolytic enzymes.colored effluents from the textile industry have led to severe environmental pollution, and this has emerged as a global issue. the feasibility of ligninolytic enzymes for the detoxification and degradation of textile wastewater was investigated. ganoderma lucidum crude ligninolytic enzymes extract (mnp 717.7, lip 576.3, and laccase 323.2 iu/ml) was produced using solid-state culture using wheat bran as substrate. the biodegradation treatment efficiency was evaluated on the basis of degradation ...201627191553
aluminum resistance in wheat involves maintenance of leaf ca(2+) and mg(2+) content, decreased lipid peroxidation and al accumulation, and low photosystem ii excitation pressure.the phytotoxic aluminum species (al(3+)) is considered as the primary factor limiting crop productivity in over 40 % of world's arable land that is acidic. we evaluated the responses of two wheat cultivars (triticum aestivum l.) with differential al resistance, cv. yecora e (al-resistant) and cv. dio (al-sensitive), exposed to 0, 37, 74 and 148 μm al for 14 days in hydroponic culture at ph 4.5. with increasing al concentration, leaf ca(2+) and mg(2+) content decreased, as well as the effective q ...201627188757
nested association mapping of stem rust resistance in wheat using genotyping by sequencing.we combined the recently developed genotyping by sequencing (gbs) method with joint mapping (also known as nested association mapping) to dissect and understand the genetic architecture controlling stem rust resistance in wheat (triticum aestivum). ten stem rust resistant wheat varieties were crossed to the susceptible line lmpg-6 to generate f6 recombinant inbred lines. the recombinant inbred line populations were phenotyped in kenya, south africa, and st. paul, minnesota, usa. by joint mapping ...201627186883
influence of salinity and temperature on uptake of perfluorinated carboxylic acids (pfcas) by hydroponically grown wheat (triticum aestivum l.).perfluoroalkyl substances (pfass) have recently attracted increasing concerns due to their ubiquitous existence, adverse effects and persistence in environment. this study employed four perfluorinated carboxylic acids (pfcas) to examine effects of salinity and temperature on the pfas uptake in wheat, one of the major crops in the north china plain. wheat plants were grown in the spiked-pfca hydroponic culture system at different salinities and temperatures. as expected, salinity and temperature ...201627186505
[the inheritance of endosperm storage proteins by the line of the saratovskaya 29 cultivar of common wheat from its parental forms].we ran a comparative analysis of storage proteins (gliadins, high- (hmw) and low-molecular-weight (lmw) glutenins, puroindolines, and exogenous α-amylase pest inhibitors) in the saratovskaya 29 cultivar line from the collection of a genetic engineering laboratory, its parental forms (albidum 24 and lyutescens 55/11), and distant ancestors (poltavka, selivanovskiy rusak, sarroza, and tetraploid beloturka). it was confirmed that the allelic states of storage proteins in the gli-1, gli-2 and glu-1 ...201627183793
an aegilops ventricosa translocation confers resistance against root-knot nematodes to common wheat.root knot nematodes (rkn; meloidogyne spp.) cause severe losses worldwide to a wide range of crops. crop rotations with resistant hosts can be used to control losses, but the wide host range of rkn limits this option. in this study, we found that the wheat cultivar lassik is resistant to several isolates of the rkn species m. incognita and m. javanica, including those that can reproduce on tomato with the resistance gene mi-1. comparison of near-isogenic lines of wheat showed that the wheat resi ...201727182071
characterization and genetic analysis of a novel light-dependent lesion mimic mutant, lm3, showing adult-plant resistance to powdery mildew in common wheat.lesion mimics (lms) that exhibit spontaneous disease-like lesions in the absence of pathogen attack might confer enhanced plant disease resistance to a wide range of pathogens. the lm mutant, lm3 was derived from a single naturally mutated individual in the f1 population of a 3-1/jing411 cross, backcrossed six times with 3-1 as the recurrent parent and subsequently self-pollinated twice. the leaves of young seedlings of the lm3 mutant exhibited small, discrete white lesions under natural field c ...201627175509
morphological and sequence-related amplified polymorphism-based molecular diversity of local and exotic wheat genotypes.assessing genetic diversity is a prerequisite for the genetic improvement of wheat. molecular markers offer accurate and reproducible means for assessing genetic diversity. field performance and sequence-related amplified polymorphism (srap)-based assessment of molecular diversity was carried out on a set of 10 local and introduced bread wheat (triticum sativum l.) genotypes grown in the middle arid region of saudi arabia. the results revealed highly significant differences among the studied phe ...201627173279
plant density effect on grain number and weight of two winter wheat cultivars at different spikelet and grain positions.in winter wheat, grain development is asynchronous. the grain number and grain weight vary significantly at different spikelet and grain positions among wheat cultivars grown at different plant densities. in this study, two winter wheat (triticum aestivum l.) cultivars, 'wennong6' and 'jimai20', were grown under four different plant densities for two seasons, in order to study the effect of plant density on the grain number and grain weight at different spikelet and grain positions. the results ...201627171343
transcriptome analysis of purple pericarps in common wheat (triticum aestivum l.).wheat (triticum aestivum l.) cultivars possessing purple grain arethought to be more nutritious because of high anthocyanin contents in the pericarp. comparative transcriptome analysis of purple (cv gy115) and white pericarps was carried out using next-generation sequencing technology. there were 23,642 unigenes significantly differentially expressed in the purple and white pericarps, including 9945 up-regulated and 13,697 down-regulated. the differentially expressed unigenes were mainly involve ...201627171148
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