Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| isolation of additional bacteriophages with genomes of segmented double-stranded rna. | eight different bacteriophages were isolated from leaves of pisum sativum, phaseolus vulgaris, lycopersicon esculentum, daucus carota sativum, raphanus sativum, and ocimum basilicum. all contain three segments of double-stranded rna and have genomic-segment sizes that are similar but not identical to those of previously described bacteriophage phi6. all appear to have lipid-containing membranes. the base sequences of some of the viruses are very similar but not identical to those of phi6. three ... | 1999 | 10419946 |
| carbon uptake and the metabolism and transport of lipids in an arbuscular mycorrhiza | both the plant and the fungus benefit nutritionally in the arbuscular mycorrhizal symbiosis: the host plant enjoys enhanced mineral uptake and the fungus receives fixed carbon. in this exchange the uptake, metabolism, and translocation of carbon by the fungal partner are poorly understood. we therefore analyzed the fate of isotopically labeled substrates in an arbuscular mycorrhiza (in vitro cultures of ri t-dna-transformed carrot [daucus carota] roots colonized by glomus intraradices) using nuc ... | 1999 | 10364411 |
| molecular characterization of dau c 1, the bet v 1 homologous protein from carrot and its cross-reactivity with bet v 1 and api g 1. | up to 70% of patients with birch pollen allergy exhibit the so-called oral allergy syndrome, an ige-mediated food allergy. the most frequent and therefore best characterized pollen-fruit syndrome is apple allergy in patients suffering from tree pollen-induced pollinosis. the occurrence of adverse reactions to proteins present in vegetables such as celery and carrots in patients suffering from pollen allergy has also been reported. cdnas for bet v 1 homologous proteins have been cloned from celer ... | 1999 | 10336602 |
| isolation and characterization of a novel antifreeze protein from carrot (daucus carota). | a modified assay for inhibition of ice recrystallization which allows unequivocal identification of activity in plant extracts is described. using this assay a novel, cold-induced, 36 kda antifreeze protein has been isolated from the tap root of cold-acclimated carrot (daucus carota) plants. this protein inhibits the recrystallization of ice and exhibits thermal-hysteresis activity. the polypeptide behaves as monomer in solution and is n-glycosylated. the corresponding gene is unique in the carr ... | 1999 | 10333479 |
| changed properties of the cytoplasmic matrix associated with desiccation tolerance of dried carrot somatic embryos. an in situ fourier transform infrared spectroscopic study | abscisic acid-pretreated carrot (daucus carota) somatic embryos survive dehydration upon slow drying, but fast drying leads to poor survival of the embryos. to determine whether the acquisition of desiccation tolerance is associated with changes in the physical stability of the cytoplasm, in situ fourier transform infrared microspectroscopy was used. although protein denaturation temperatures were similar in the embryos after slow or fast drying, the extent of the denaturation was greater after ... | 1999 | 10318693 |
| a leucine-rich repeat protein of carrot that exhibits antifreeze activity. | a gene encoding an antifreeze protein (afp) was isolated from carrot (daucus carota) using sequence information derived from the purified protein. the carrot afp is highly similar to the polygalacturonase inhibitor protein (pgip) family of apoplastic plant leucine-rich repeat (lrr) proteins. expression of the afp gene is rapidly induced by low temperatures. furthermore, expression of the afp gene in transgenic arabidopsis thaliana plants leads to an accumulation of antifreeze activity. our findi ... | 1999 | 10214940 |
| production of glomus intraradices propagules, an arbuscular mycorrhizal fungus, in an airlift bioreactor | this work addresses the symbiotic culture of the arbuscular mycorrhizal (am) fungus glomus intraradices with daucus carota hairy roots transformed by agrobacterium rhizogenes, in two submerged culture systems: petri dish and airlift bioreactor. am fungi play an active role in plant nutrition and protection against plant pathogens. these fungi are obligate biotrophs as they depend on a host plant for their needs in carbohydrates. the effect of the mycorrhizal roots inoculum-to-medium volume ratio ... | 1999 | 10099599 |
| tissue-specific expression of two genes for sucrose synthase in carrot (daucus carota l.). | sucrose synthase, which cleaves sucrose in the presence of uridine diphosphate (udp) into udp-glucose and fructose, is thought to be a key determinant of sink strength of heterotrophic plant organs. to determine the roles of the enzyme in carrot, we characterized carrot sucrose synthase at the molecular level. two genes (susy*dc1 and susy*dc2) were isolated. the deduced amino acid sequences are 87% identical. however, the sequences upstream of the translation initiation codons are markedly diffe ... | 1999 | 10080700 |
| [chemical, chemosensory and human-sensory experiments on taste and flavor of carrots]. | the relationship between sensory quality of carrots and their contents and composition of essential oils and total sugars as influenced by nitrogen fertilization was investigated. carrots (daucus carota l.) of the variety 'nanthya' f1 (s&g sandoz seeds) were grown in weihenstephan 1996 with three levels of inorganic nitrogen fertilization (3 levels in 4 replications). medium- and higher-boiling flavour-components were extracted as essential oils and separated gas-chromatographically (gc-fid). lo ... | 1998 | 9881369 |
| somatic and zygotic embryos of daucus carota l. display different protein patterns until conversion to plants. | total protein patterns of different developmental stages of carrot zygotic and somatic embryos revealed by one- and two-dimensional gel electrophoresis were compared using statistical dissimilarity index matrix, and some major polypeptides were partially sequenced. in spite of similar morphology, the protein patterns of somatic embryos at the torpedo stage were clearly different from those of zygotic embryos. in particular, none of the proteins specific of zygotic embryos required for maturation ... | 1998 | 9871368 |
| characterization of source- and sink-specific sucrose/h+ symporters from carrot. | to understand how sucrose (suc) is transported from source leaves to developing tap roots of carrot (daucus carota l.), we cloned two cdnas (dcsut1 and dcsut2) for proteins with homologies to plant suc/h+ symporters. the deduced polypeptide sequences are 52% identical and have 12 predicted membrane-spanning domains each. transport activities were confirmed by expression of the clones in yeast cells. both transporters had optimal activity below ph 5.0 and michaelis constant values of 0.5 mm. suc ... | 1998 | 9847123 |
| determination of provitamin a of green leafy vegetables by high performance liquid chromatography and open column chromatography. | the purpose of study was to determine the provitamin a value through beta-carotene analysis of five brazilian leafy vegetables: carrot leaves (daucus carota l.), beet leaves (beta vulgaris l.) "serralha" (sonchus oleracea l.), mint (mentha piperita ll.) and celery leaves (aplum graveolens. l.). two analysis methods were used: open-column chromatography (occ) and high performance liquid chromatography (hplc). two analysis methods were used: open-column chromatography (occ) and high performance li ... | 2013 | 9795484 |
| elongation factor-1alpha stabilizes microtubules in a calcium/calmodulin-dependent manner. | elongation factor-1alpha (ef-1alpha), a highly conserved protein named for its role in protein translation, is also a microtubule-associated protein (map). we used high-resolution differential interference contrast microscopy to quantify the effect of substoichiometric amounts of ef-1alpha (isolated from daucus carota) on the dynamic instability of microtubules assembled in vitro from either animal or plant tubulin. ef-1alpha modulates the dynamic behavior of microtubules assembled from either t ... | 1998 | 9786091 |
| natural genetic transformation by agrobacterium rhizogenes . annual flowering in two biennials, belgian endive and carrot | genetic transformation of belgian endive (cichorium intybus) and carrot (daucus carota) by agrobacterium rhizogenes resulted in a transformed phenotype, including annual flowering. back-crossing of transformed (r1) endive plants produced a line that retained annual flowering in the absence of the other traits associated with a. rhizogenes transformation. annualism was correlated with the segregation of a truncated transferred dna (t-dna) insertion. during vegetative growth, carbohydrate reserves ... | 1998 | 9765539 |
| regulation of enzymes involved in anthocyanin biosynthesis in carrot cell cultures in response to treatment with ultraviolet light and fungal elicitors. | the accumulation of anthocyanins in cell cultures of daucus carota l. and the enzymes involved in their biosynthesis were investigated under growth in the dark, continuous irradiation with uv light, incubation with elicitors from pythium aphanidermatum, and elicitor treatment of uv-irradiated cells. upon uv irradiation, anthocyanin accumulation was strongly enhanced, and the enzymes of the phenylpropanoid and flavonoid pathways, including the "late" enzymes cyanidin galactosyltransferase, cyanid ... | 1998 | 9684371 |
| two isoforms of plant rad23 complement a uv-sensitive rad23 mutant in yeast. | dna repair by nucleotide excision (ner) has been demonstrated in plant cells at the biochemical level but until now none of the molecular components of the plant ner complex has been identified. in this paper, the cloning and characterization of two isoforms of rad23 from carrot (daucus carota l.) are reported. it has been suggested that rad23 in yeast is an assembly factor of the ner complex required for transcription-coupled repair as well as efficient overall genome repair. a functional assay ... | 1998 | 9681019 |
| phosphoglycerylethanolamine posttranslational modification of plant eukaryotic elongation factor 1alpha. | eukaryotic elongation factor 1alpha (eef-1a) is a multifunctional protein. there are three known posttranslational modifications of eef-1a that could potentially affect its function. except for phosphorylation, the other posttranslational modifications have not been demonstrated in plants. using matrix-assisted laser desorption/ionization-mass spectrometry and peptide mass mapping, we show that carrot (daucus carota l.) eef-1a contains a phosphoglycerylethanolamine (pge) posttranslational modifi ... | 1998 | 9662537 |
| a chromo box gene from carrot (daucus carota l.): its cdna structure and expression during somatic and zygotic embryogenesis. | a cdna clone, designated dcdb1, was isolated from a cdna library prepared from embryogenic cell clusters of carrot (daucus carota l.) and characterized. the cdna (1416 bp) encoded for a protein of 392 amino acid residues that contained a conserved chromo domain. the chromo domain is a 37 aa region found in both the polycomo gene product, which is a repressor of homeotic genes, and a heterochromatin protein 1 of drosophila. this domain is postulated to function in the binding of proteins to chrom ... | 1998 | 9602049 |
| expression pattern of the carrot ep3 endochitinase genes in suspension cultures and in developing seeds | carrot (daucus carota) extracellular protein 3 (ep3) class iv endochitinases were previously identified based on their ability to rescue somatic embryos of the temperature-sensitive cell line ts11. whole-mount in situ hybridization revealed that a subset of the morphologically distinguishable cell types in embryogenic and nonembryogenic suspension cultures, including ts11, express ep3 genes. no expression was found in somatic embryos. in carrot plants ep3 genes are expressed in the inner integum ... | 1998 | 9576773 |
| rolb expression pattern in the early stages of carrot somatic embryogenesis. | the pattern of expression of the rolb gene, derived from the t-dna of the plant pathogen agrobacterium rhizogenes, has been investigated during the early stages of somatic embryo formation in suspension cultures of carrot (daucus carota l.). the reporter gene gus (escherichia coli beta-glucuronidase), under transcriptional control of full-length rolb promoter region, has been utilized in order to evaluate both qualitative and quantitative variations in the expression pattern. fluorimetric measur ... | 1997 | 9570894 |
| an apparent case of nonsymmetrical and sustained strand-specific hemimethylation in the dc8 gene of carrot. | the dc8 gene of carrot (daucus carota l.) shows differential expression during embryo development. changes in methylation patterns of a segment of about 500 bp (from base +120 to base -446) of dc8 allele 6 were investigated by treating genomic dna, extracted from embryogenic callus at different stages of development, with sodium bisulfite to modify nonmethylated cytosines. following asymmetric (strand-specific) amplification, base sequences for samples from each developmental stage were determin ... | 1998 | 9549056 |
| intracellular ph in arbuscular mycorrhizal fungi. a symbiotic physiological marker | a method was developed to perform real-time analysis of cytosolic ph of arbuscular mycorrhizal fungi in culture using dye and ratiometric measurements (490/450 nm excitations). the study was mainly performed using photometric analysis, although some data were confirmed using image analysis. the use of nigericin allowed an in vivo calibration. experimental parameters such as loading time and concentration of the dye were determined so that ph measurements could be made for a steady-state period o ... | 1998 | 9536044 |
| characterization of storage proteins in daucus carota l.: two novel proteins display zygotic embryo specificity. | although maturation-related proteins are well known in the endosperm of albuminous seeds, an important question is whether the zygotic embryo possesses its own maturation proteins. we report on the isolation and partial characterization of storage proteins of carrot (daucus carota l. var nandor) dry achenes and isolated zygotic embryos, using one- and two-dimensional electrophoresis techniques, hplc and amino acid sequencing. the presence of a series of abundant polypeptides showing charge heter ... | 1998 | 9517000 |
| the ovary of the desert locust schistocerca gregaria contains a glycine- and proline-rich peptide that displays sequence similarities with a new class of gprp proteins from plants. | a novel, highly hydrophobic, glycine- and proline-rich peptide was characterized in the ovary of the desert locust, schistocerca gregaria. the peptide was detected as one of the major peaks in a chromatographic separation of an acidic methanolic extract of 50 mature ovaries. electrospray mass spectrometry yielded a molecular mass of 6305 da. the partial amino acid sequence as determined by edman degradation based automated microsequencing is as follows: ala-tyr-pro-ala-his-gln-gly-tyr- pro-ala-h ... | 1998 | 9480819 |
| metabolism of polyamines in transgenic cells of carrot expressing a mouse ornithine decarboxylase cdna. | the metabolisms of arginine (arg), ornithine (orn), and putrescine were compared in a nontransgenic and a transgenic cell line of carrot (daucus carota l.) expressing a mouse orn decarboxylase cdna. [14c]arg, [14c]orn, and [14c]putrescine were fed to cells and their rates of decarboxylation, uptake, metabolism into polyamines, and incorporation into acid-insoluble material were determined. transgenic cells showed higher decarboxylation rates for labeled orn than the nontransgenic cells. this was ... | 1998 | 9449846 |
| atltp1 luciferase expression during carrot somatic embryogenesis. | the carrot (daucus carota l.) ep2 gene encodes a lipid transfer protein (ltp) which is expressed during protoderm formation in developing embryos. to develop a vital reporter system for gene expression during somatic embryo development a 1.1 kb fragment of the arabidopsis thaliana ltp1 promoter was fused to the firefly luciferase (luc) coding sequence. the atltp1 luciferase expression pattern in transformed carrot suspension cultures was identical to the expression pattern of the endogenous carr ... | 1997 | 9418058 |
| malondialdehyde and 4-hydroxy-2-nonenal in plant tissue cultures: lc-ms determination of 2,4-dinitrophenylhydrazone derivatives. | the cytologically active secondary lipid peroxidation products, malondialdehyde (mda) and 4-hydroxy-2-nonenal (hne) have been detected as their 2,4-dinitrophenylhydrazone (dnp) derivatives in plant tissue cultures using lc-ms. this paper reports, for the first time, the use of lc-ms methodology to definitively identify 4-hydroxy-2-nonenal in plants. limits of detection for the two derivatives are approximately 5 pmol (1.2 x 10(-9) g; 1 microm) and 0.1 pmol (3 x 10(-11) g; 20 nm) respectively. ma ... | 1997 | 9350430 |
| action spectrum for induction of promoter activity of phenylalanine ammonia-lyase gene by uv in carrot suspension cells. | the full-length promoter (-2335) of the carrot (daucus carota) phenylalanine ammonia-lyase gene (gdcpal1) fused to the luciferase reporter gene was transiently transformed to carrot protoplasts by electroporation, and the promoter activity induced by monochromatic uv light of various wavelengths was examined. the action spectrum constructed from the fluence-response curves showed a single peak at around 280 nm, suggesting that the activation of the gdcpal1 promoter is categorizable as one of the ... | 1997 | 9337617 |
| anti-steroidogenic activity of the petroleum ether extract and fraction 5 (fatty acids) of carrot (daucus carota l.) seeds in mouse ovary. | the petroleum ether extract and fraction 5 (fatty acids) of carrot seeds arrested the normal estrus cycle of adult mouse and reduced the weight of ovaries significantly. the cholesterol and ascorbic acid content in ovaries were significantly elevated due to the treatment with extract and fraction 5 (fatty acids) of carrot seeds. the significant inhibition of delta 5,3-beta-hydroxy steroid dehydrogenase and glucose-6-phosphate dehydrogenase, the two key enzymes involved in ovarian steroidogenesis ... | 1997 | 9292415 |
| an extracellular insoluble inhibitor of cysteine proteinases in cell cultures and seeds of carrot. | an 18 kda extracellular insoluble protein (eip18) was found previously in amorphous particles suspended in the culture medium and in the interspaces of cell clusters of carrot (daucus carota l.) callus, as well as in the extracellular spaces of carrot seeds, being located both in the embryo and at the inner edge of the endosperm. we purified eip18 by washing the amorphous particles with the mixture of triton x-100, nacl and ethylenediaminetetraacetic acid (edta). we determined several partial am ... | 1997 | 9177316 |
| peripheral framework of carrot cell nucleus contains a novel protein predicted to exhibit a long alpha-helical domain. | a monoclonal antibody, cml-1, raised against carrot (daucus carota l.) nuclear-matrix proteins selectively labeled the nuclear periphery of carrot protoplasts when visualized by confocal and electron microscopy. to identify the constituent proteins of higher plant cells structurally homologous to the vertebrate nuclear lamina, we cloned overlapping cdnas partially encoding a cml-1-recognized protein and determined the entire sequence including the open reading frame. when the deduced amino acid ... | 1997 | 9141634 |
| rhamnogalacturonan ii, a dominant polysaccharide in juices produced by enzymic liquefaction of fruits and vegetables. | rhamnogalacturonan ii (rg-ii), a small complex pectic polysaccharide, is released from apple (malus domestica), carrot (daucus carota), and tomato (solanum lycopersicum) by treatment with two commercial liquefying enzyme preparations. rg-ii was isolated by size-exclusion chromatography from apple, tomato, and carrot juices obtained by enzymic liquefaction. all the rg-iis contained the diagnostic sugars, apiose, 2-o-methyl-l-fucose, 2-o-methyl-d-xylose, aceric acid, kdo and dha. glycosyl-linkage ... | 1997 | 9060185 |
| plant 21d7 protein, a nuclear antigen associated with cell division, is a component of the 26s proteasome. | previously, we cloned a carrot (daucus carota l.) cdna encoding a 45-kd protein, 21d7, located in the nuclei of proliferating cells. the 21d7 protein is similar to the partial sequence of a regulatory subunit of the bovine 26s proteasome, p58 (g. demartino, c.r. moomaw, o.p. zagnitko, r.j. proske, m. chu-ping, s.j. afendis, j.c. swaffield, c.a. slaughter [1994] j biol chem 269: 20878-20884) and to the deduced sequence encoded by the saccharomyces cerevisiae gene sun2 (m. kawamura, k. kominami, j ... | 1997 | 9008397 |
| cloning of a cdna encoding dna topoisomerase i in daucus carota and expression analysis in relation to cell proliferation. | dna topoisomerase i is an enzyme involved in several processes related to dna metabolism. despite the physiological importance, the regulation of top1 gene expression has not yet been investigated in plants. in order to monitor the possible correlation between levels of top1 transcripts and the proliferative state of the cell, two partially overlapping cdnas encoding dna topoisomerase i from daucus carota have been isolated from a poly(a)(+)-primed library, using an arabidopsis thaliana probe, a ... | 1996 | 8996105 |
| purification and characterization of neutral and alkaline invertase from carrot. | neutral and alkaline invertase were identified in cells of a suspension culture of carrot (daucus carota l.) and purified to electrophoretic homogeneity. neutral invertase is an octamer with a molecular mass of 456 kd and subunits of 57 kd, whereas alkaline invertase is a tetramer with a molecular mass of 504 kd and subunits of 126 kd. both enzymes had sharp ph profiles, with maximal activities at ph 6.8 for neutral invertase and ph 8.0 for alkaline invertase, and both hydrolyzed sucrose with ty ... | 1996 | 8972597 |
| isolation and characterization of a diverse set of genes from carrot somatic embryos. | the early events in plant embryogenesis are critical for pattern formation, since it is during this process that the primary apical meristems and the embryo polarity axis are established. however, little is known about the molecular events that are unique to the early stages of embryogenesis. this study of gene expression during plant embryogenesis is focused on identifying molecular markers from carrot (daucus carota) somatic embryos and characterizing the expression and regulation of these gen ... | 1996 | 8938424 |
| purification and characterization of a wound-inducible cell wall cationic peroxidase from carrot roots. | we have isolated a novel cell wall, cationic peroxidase (pi > 9.3) from roots of the carrot plant, daucus carota. the purified isozyme, referred to as cp > 9.3, has a molecular mass of 45 kilodaltons and an reinheitzahl value of 2.3. amino-acid composition analysis and n-terminal sequencing have been performed with cp > 9.3. the n-terminal sequence shows no homology to any sequence in the protein and nucleic acid data banks. cp > 9.3 activity is induced by wounding in carrot leaves and petioles; ... | 1996 | 8806623 |
| visualization of plant cell walls by atomic force microscopy. | atomic force microscopy has been used to visualize the ultrastructure of hydrated plant cell wall material from prepared apple (malus pumila mill; cox orange pippin), water chestnut (eleocharis dulcis l.), potato (solanum tuberosum l.; bintje), and carrot (daucus carota l.; amsterdamse bak) parenchyma. samples of cell wall material in aqueous suspension were deposited onto freshly cleaved mica. excess water was blotted away and the moist samples were imaged in air at ambient temperature and humi ... | 1996 | 8785273 |
| variations in the structure of neutral sugar chains in the pectic polysaccharides of morphologically different carrot calli and correlations with the size of cell clusters. | carrot (daucus carota l.) embryogenic callus (ec) loses its embryogenic competence and becomes non-embryogenic callus (nc) during long-term culture. with the loss of embryogenic competence, the cell clusters become smaller and the extent of intercellular attachments is reduced. pectic fractions prepared from ec and nc were separated into two subfractions by gel filtration. a difference in sugar composition between ec and nc was found only in the high-molecular-mass (ca. 1300 kda) subfraction, an ... | 1996 | 8727449 |
| purification and characterization of glycosyltransferases involved in anthocyanin biosynthesis in cell-suspension cultures of daucus carota l. | the major anthocyanins accumulated by an afghan cultivar of daucus carota l. are cyanidin 3-(xylosylglucosylgalactosides) acylated with sinapic or ferulic acid. the formation of the branched triglycoside present as a common structural element requires an ordered sequence of glycosylation events. two of these enzymic glycosylation reactions have been detected in protein preparations from carrot cell-suspension cultures. the first step is a galactosyl transfer catalyzed by udp-galactose: cyanidin ... | 1996 | 8717136 |
| expression of dc8 is associated with, but not dependent on embryogenesis. | dc8 is a late embryogenesis-abundant (lea) protein gene isolated from carrot (daucus carota). deletion analysis of the dc8 promoter was performed to determine the sequences required for aba and seed-specific regulation of dc8 transcription. to investigate the mechanism of dc8 expression during seed development, chimeric gene constructs containing dc8 promoter fragments fused to a promoterless beta-glucuronidase gene (dc8:gus) were introduced into carrot, tobacco (nicotiana tobacum) and arabidops ... | 1996 | 8704145 |
| structural and functional characterization of the intergenic spacer region of the rdna in daucus carota. | the intergenic spacer (igs) region of rdna in daucus carota contains at least eight kinds of repeated sequence. one sequence may act as a genuine initiation site for a stable transcript and another may act as a spacer promoter that may be an entry site for proteins required for transcription. | 1996 | 8665099 |
| callose synthesis in spirostanol treated carrot cells is not triggered by cytosolic calcium, cytosolic ph or membrane potential changes. | carrot (daucus carota l.) cell suspensions were treated with a spirostanol saponin from yucca. this saponin is an elicitor of callose synthesis. irrespectively of the mode of action of spirostanol on the callose synthase activity itself, the spirostanol-induced callose synthesis in carrot is not preceded by changes in membrane potential, cytosolic free calcium or cytosolic ph. the inability of modulators of cytosolic free calcium content (verapamil, nifedipine and br-a23187), egta and a proton p ... | 1995 | 8564297 |
| identification and expression of a cdna from daucus carota encoding a bifunctional aspartokinase-homoserine dehydrogenase. | aspartokinase (ec 2.7.2.4) and homoserine dehydrogenase (ec 1.1.1.3) catalyze steps in the pathway for the synthesis of lysine, threonine, and methionine from aspartate. homoserine dehydrogenase was purified from carrot (daucus carota l.) cell cultures and portions of it were subjected to amino acid sequencing. oligonucleotides deduced from the amino acid sequences were used as primers in a polymerase chain reaction to amplify a dna fragment using dna derived from carrot cell culture mrna as tem ... | 1993 | 8507831 |
| isolation and characterization of a new chromosomal virulence gene of agrobacterium tumefaciens. | a mutant (strain b119) of agrobacterium tumefaciens with a chromosomal mutation was isolated by transposon (tn5) mutagenesis. the mutant exhibited growth rates on l agar and minimal medium (ab) plates similar to those of the parent strain (strain a208 harboring a nopaline-type ti plasmid). the mutant was avirulent on all host plants tested: daucus carota, cucumis sativus, and kalanchoe diagremontiana. the mutant was not impaired in attachment ability to carrot cells. the mutant had one insertion ... | 1993 | 8491736 |
| cdna cloning of ecp40, an embryogenic-cell protein in carrot, and its expression during somatic and zygotic embryogenesis. | a cdna of 1.2 kb was isolated from a cdna library of embryogenic cells of carrot (daucus carota l.) by use of nucleotide sequences that encode two internal amino-acid sequences of ecp40 (an embryogenic-cell protein with a relative molecular mass of 40,000). a genomic southern blot using the cdna as probe suggested that there are at least two genes for ecp40 in the carrot genome. the cdna encoded an open reading frame of 306 amino acids, and the deduced amino-acid sequence was found to share two ... | 1993 | 8490126 |
| peptide splicing in the vacuolar atpase subunit a from candida tropicalis. | subunit a of the vacuolar proton pump appears to be responsible for the atp hydrolysis which is coupled to the pumping of protons into a variety of intracellular acid compartments, including the fungal vacuole. we report here the cloning and sequence determination of the gene encoding subunit a from candida tropicalis. southern blot hybridization analysis indicates that there is a single gene which encodes this protein. the gene contains a single intron at the extreme 5'-end of the coding region ... | 1993 | 8463270 |
| [granulated carrots (daucus carota) in dog nutrition]. | in feeding and balance trials with dogs (4) dried, ground carrots were tested in addition (30%) to a basic dry food (rice, corn gluten, fish meal and supplements). the palatability of both mixtures was good: fecal water losses were increased after feeding the carrots. apparent digestibilities of organic and inorganic matter, especially sodium and potassium (not phosphorus), decreased. digestibility of carrots was calculated to reach 70% with 1.2 mj of digestible energy per 100 g dry matter. redu ... | 1993 | 8430292 |
| purification and characterization of 3-methylcrotonyl-coa carboxylase from somatic embryos of daucus carota. | 3-methylcrotonyl-coa carboxylase, a biotin enzyme, was purified from embryos of daucus carota. polyethylene glycol precipitation and monomeric avidin affinity chromatography were used to purify all biotin enzymes from cell-free extracts of embryos. the resulting 3-methylcrotonyl-coa carboxylase preparation had a specific activity of 745 nmol/min.mg protein, representing a 3725-fold purification of the enzyme and a 135% recovery of activity. fractionation of the purified biotin-containing protein ... | 1993 | 8393642 |
| stimulation of calcium influx and calcium cascade by cyclic amp in cultured carrot cells. | treatment of cultured carrot (daucus carota l.) cells with activators of adenylate cyclase, forskolin, and cholera toxin induced the biosynthesis of an antifungal isocoumarin, 6-methoxymellein, in the cells. addition of dibutyryl cyclic amp to carrot cell culture also stimulated the accumulation of the compound. the cyclic amp-evoked 6-methoxymellein production was significantly depressed in the presence of certain inhibitors of calcium cascade such as ca2+ channel blockers and inhibitors of cal ... | 1993 | 8385897 |
| molecular cloning and analysis of a cdna coding for the bifunctional dihydrofolate reductase-thymidylate synthase of daucus carota. | molecular cloning of dihydrofolate reductase-thymidylate synthase (dhfr-ts) of daucus carota was achieved by immunoscreening of a cdna library obtaining a 2 kbp clone which contains an open reading frame of 1528 bp. comparison of the deduced amino acid sequence with those from other sources revealed the presence of motifs typical of dhfr and ts thus confirming the bifunctional nature of the carrot protein. as in other organisms, a higher degree of conservation was observed in the ts domain. anal ... | 1993 | 8329682 |
| isolation and characterization of a cdna clone for plant nuclear antigen 21d7 associated with cell division. | a cdna clone was isolated from a carrot (daucus carota l.) cdna expression library using monoclonal antibody 21d7, which recognizes a nuclear antigen associated with cell division in plant cells. to show that the isolated cdna encodes the 21d7 antigen, a polyclonal antiserum was raised against a recombinant fusion protein specified by the cdna. both the polyclonal antiserum and the monoclonal antibody 21d7 recognized the same plant protein on immunoblots, in immunoprecipitation experiments, and ... | 1993 | 8310059 |
| expression of dcprp1 is linked to carrot storage root formation and is induced by wounding and auxin treatment. | a carrot (daucus carota, l.) genomic clone (dcprp1) was isolated on the basis of its homology to previously described cdnas encoding a wound-inducible, proline-rich cell wall protein. dna sequence analysis showed that dcprp1 contains a single open-reading frame encoding a 235-amino acid protein that is colinear with that predicted from the cdna sequence with the exception of four amino acids at the n terminus and a 60-nucleotide insertion present within the genomic clone. genomic southern hybrid ... | 1993 | 8278498 |
| characterization of the non-specific lipid transfer protein ep2 from carrot (daucus carota l.). | the extracellular protein ep2 was previously identified as non-specific lipid transfer protein based on its cdna-derived amino acid sequence. here, the purification of the ep2 protein from the medium of somatic embryo cultures is described. after two cycles of ion-exchange and gel permeation chromatography, a single silver-stained protein band with an apparent molecular mass of 10 kda was observed on sds-page. this protein band was recognized by the antiserum raised against a ep2-beta-galactosid ... | 2006 | 8232259 |
| a trna gene mapping within the chloroplast rdna cluster is differentially expressed during the development of daucus carota. | in vivo analysis of expression of the chloroplast rdna cluster during somatic embryogenesis of daucus carota (d.carota) was performed by northern-blot analysis with different dna probes, spanning both the 16s rrna gene, the 16s-23s rrna spacer, which contains the two mosaic trna genes trna(ile) and trna(ala), and the region upstream of the 16s rrna gene, where a trna(val) maps. we show that expression both of the spacer trnas trna(ile) and trna(ala) is not significantly regulated during developm ... | 1994 | 8202376 |
| characterization of a gene that is expressed early in somatic embryogenesis of daucus carota. | the emb-1 mrna of carrot (daucus carota) was isolated as an embryo abundant cdna clone (t.h. ulrich, e.s. wurtele, b.j. nikolau [1990] nucleic acids res 18: 2826). northern analyses of rna isolated from embryos, cultured cells, and a variety of vegetative organs indicate that the emb-1 mrna specifically accumulates in embryos, beginning at the early stages of embryo development. in situ hybridization with both zygotic and somatic embryos show that the emb-1 mrna begins to accumulate at low level ... | 1993 | 8108498 |
| voltage-dependent calcium-permeable channels in the plasma membrane of a higher plant cell. | numerous biological assays and pharmacological studies on various higher plant tissues have led to the suggestion that voltage-dependent plasma membrane ca2+ channels play prominent roles in initiating signal transduction processes during plant growth and development. however, to date no direct evidence has been obtained for the existence of such depolarization-activated ca2+ channels in the plasma membrane of higher plant cells. carrot suspension cells (daucus carota l.) provide a well-suited s ... | 1994 | 8039493 |
| changes in phosphatidylinositol metabolism in response to hyperosmotic stress in daucus carota l. cells grown in suspension culture. | carrot (daucus carota l.) cells plasmolyzed within 30 s after adding sorbitol to increase the osmotic strength of the medium from 0.2 to 0.4 or 0.6 osmolal. however, there was no significant change in the polyphosphorylated inositol phospholipids or inositol phosphates or in inositol phospholipid metabolism within 30 s of imposing the hyperosmotic stress. maximum changes in phosphatidylinositol 4-monophosphate (pip) metabolism were detected at 5 min, at which time the cells appeared to adjust to ... | 1993 | 8029337 |
| novel o-d-galacturonoyl esters in the pectic polysaccharides of suspension-cultured plant cells. | driselase digestion of uronate-6-14c-labeled primary walls of cultured spinach (spinacia oleracea l.) cells yielded about 18 novel uronate-containing compounds, most of which could be hydrolyzed by cold dilute alkali to yield oligo-[14c]galacturonides. one typical driselase digestion product (compound 17) yielded alpha-(1-->4)-d-[14c]galacturonotriose(gala3) upon very mild treatment with alkali (50% yield of gala3 in 7.2 min at ph 11 and 25 degrees c). one of the three galacturonate residues in ... | 1993 | 8022945 |
| a cdna differentially expressed between embryogenic and nonembryogenic carrot (daucus carota l.) cells. | 1993 | 8022926 | |
| [the in vitro action of plants on vibrio cholerae]. | natural products of several plants, according to the geographic location, are used by peruvian people in the popular treatment of diarrhea, with good success. when cholerae cases appeared in peru, we were interested to know the "in vitro" effect against vibrio cholerae 01, of these useful plants to treat diarrhea. the following plants were tested: cichorium intybus, althaea officinalis, psorela glandulosa, geranium maculatum, punica granatum, malus sativa, cydonia oblonga, chenopodium ambrosoide ... | 1994 | 8018898 |
| cdna cloning of carrot (daucus carota) soluble acid beta-fructofuranosidases and comparison with the cell wall isoenzyme. | carrot (daucus carota), like most other plants, contains various isoenzymes of acid beta-fructofuranosidase (beta f) (invertase), which either accumulate as soluble polypeptides in the vacuole (isoenzymes i and ii) or are ionically bound to the cell wall (extracellular beta f). using antibodies against isoenzyme i of carrot soluble beta f, we isolated several cdna clones encoding polypeptides with sequences characteristic of beta fs, from bacteria, yeast, and plants. the cdna-derived polypeptide ... | 1994 | 8016265 |
| analysis of the rolc promoter region involved in somatic embryogenesis-related activation in carrot cell cultures. | in cell cultures of carrot (daucus carota l.), somatic embryogenesis can be induced by transferring cells from a medium containing 2,4-dichlorophenoxyacetic acid (2,4-d) to one devoid of 2,4-d. previous analysis of transgenic carrot cells containing the 5' non-coding sequence of the ri plasmid rolc and a structural gene for bacterial beta-glucuronidase (uida) has shown that the chimeric gene is actively expressed after induction of somatic embryogenesis. in this study, we demonstrate that activa ... | 1994 | 8016259 |
| the highly edited orf206 in oenothera mitochondria may encode a component of a heme transporter involved in cytochrome c biogenesis. | a highly transcribed region in oenothera mitochondria codes for a reading frame (orf206) which shows high homology to the marchantia encoded mitochondrial open reading frame orf277 and is also conserved in the mitochondrial genomes of arabidopsis thaliana and daucus carota. transcripts of orf206 are modified by cytidine to uridine changes in 46 positions by rna editing, affecting 30% of all cytidines and 15% of the total encoded amino acids. this orf is cotranscribed with an upstream reading fra ... | 1994 | 8003696 |
| inositol(1,4,5)trisphosphate production in plant cells: stimulation by the venom peptides, melittin and mastoparan. | the ability of the amphiphilic peptides, melittin and mastoparan, to modulate the production of inositol(1,4,5)trisphosphate in cultured plant (daucus carota l.) cells was investigated. when added to intact cells melittin and mastoparan caused a rapid and dose-dependent increase in inositol(1,4,5)trisphosphate concentrations. in isolated protoplasts, inositol(1,4,5)trisphosphate levels were 12- to 16-fold higher than in the corresponding cells and neither melittin nor mastoparan was able to sign ... | 1994 | 7999106 |
| mechanisms of the proliferation and differentiation of plant cells in cell culture systems. | plant cell functions have been investigated in various cell culture systems. in this review, we summarize results obtained from investigations of gene expression during the cell cycle in synchronized cultures of catharanthus roseus during somatic embryogenesis in suspension cultures of daucus carota, during organogenesis in tissue cultures of arabidopsis thaliana and during the transdifferentiation of isolated mesophyll cells to tracheary elements in single-cell cultures of zinnia elegans. | 1994 | 7981037 |
| high-performance liquid chromatographic determination of dietary fibre in raw and processed carrots. | an hplc method was developed to determine dietary fibre in carrots (daucus carota l.). primary hydrolysis of dietary fibre residue was performed with 12 m h2so4 for 2 h at 40 degrees c and secondary hydrolysis with 0.414 m h2so4 for 3 h at 100 degrees c. for the neutralization step prior to injection, ag4-x4 resin (bio-rad) was used. neutral monosaccharides were separated using an hpx-87p column (bio-rad). this method was applied for evaluation of the quantitative variation of dietary fibre cont ... | 1994 | 7921187 |
| orf250 encodes a second subunit of an abc-type heme transporter in oenothera mitochondria. | a highly transcribed region in oenothera mitochondria codes for an open reading frame comprising 250 condons (orf250). this open reading frame shows high sequence similarity to the helc gene of rhodobacter capsulatus which encodes a subunit of a proposed abc-type heme transporter. transcripts of orf250 are edited by cytidine to uridine transitions at 29 sites, altering 10% of all encoded amino acids. genes homologous to helc have also been found in the bacteria bradyrhizobium japonicum and esche ... | 1995 | 7862087 |
| molecular genetics of the maize (zea mays l.) aspartate kinase-homoserine dehydrogenase gene family. | aspartate kinase (ak) and homoserine dehydrogenase (hsdh) are enzymes in the aspartate-derived amino acid biosynthetic pathway. recent biochemical evidence indicates that an ak-hsdh bifunctional enzyme exists in maize (zea mays l.). in this report, we characterize three genes that encode subunits of ak-hsdh. two cdnas, pakhsdh1 and pakhsdh2, containing the full-coding sequence, and one partial cdna, pakhsdh3, encode amino acid sequences similar to the reported monofunctional ak and hsdh enzymes ... | 1994 | 7846152 |
| biochemical, physiological, and molecular characterization of sucrose synthase from daucus carota. | sucrose synthase (ec 2.4.1.13) from carrot (daucus carota) is a tetramer with a molecular mass of 320 kd and subunits of 80 kd. the enzyme has a ph optimum of 7.0 (cleavage direction). maximal activities were measured at 55 degrees c. the km for suc was estimated as 87 mm and for udp as 0.39 mm. fructose acts as a noncompetitive inhibitor with an inhibition constant of 17.2 mm. in contrast, glucose inhibits carrot sucrose synthase uncompetitively with an inhibition constant of 4.3 mm. cdna clone ... | 1995 | 7784526 |
| cloning, nucleotide sequence and expression of the bifunctional dihydrofolate reductase-thymidylate synthase from glycine max. | cdnas encoding the bifunctional dihydrofolate reductase-thymidylate synthase from glycine max were isolated and sequenced. the 1794 base full length cdna contains a single open reading frame of 1593 bases. the predicted size of the encoded protein is 530 amino acids with a molecular weight of 59,707. the protein has two domains: a 226 residue dhfr domain in the n-terminus, which is over 30% identical to human dhfr or the dhfr domain of protozoal dhfr-ts, and a 304 residue thymidylate synthase (t ... | 1995 | 7742362 |
| association of multiple gtp-binding proteins with the plant cytoskeleton and nuclear matrix. | several types of gtp-binding proteins exist in plant cells. these include the ras-related low-molecular-weight monomeric gtp-binding proteins and the multi-subunit group which more closely resembles members of the mammalian heterotrimeric g-protein family. proteins belonging to both of these families are known to be involved in cell signalling events and have until recently been assumed to be associated predominantly with membranes. we have investigated the possibility that gtp-binding proteins ... | 1995 | 7741751 |
| the effects of mastoparan on the carrot cell plasma membrane polyphosphoinositide phospholipase c. | when [3h]inositol-labeled carrot (daucus carota l.) cells were treated with 10 or 25 microm wasp venom peptide mastoparan or the active analog mas-7 there was a rapid loss of more than 70% of [3h]phosphatidylinositol-4-monophosphate (pip) and [3h]phosphatidylinositol-4,5-bisphosphate (pip2) and a 3- and 4-fold increase in [3h]inositol-1,4-p2 and [3h]inositol-1,4,5-p3, respectively. the identity of [3h]inositol-1,4,5-p3 was confirmed by phosphorylation with inositol-1,4,5-p3 3-kinase and co-migra ... | 1995 | 7716245 |
| nucleotide sequence of the atpase a- and b-subunits of the halophilic archaebacterium haloferax volcanii and characterization of the enzyme. | using pcr with degenerate oligonucleotides, we amplified two conserved regions of the plasma membrane atpase a (alpha)- and b (beta)-subunit-encoding genes from haloferax volcanii, a halophilic archaeon. the amplified fragments were cloned and sequenced, and then used as homologous probes to clone genomic restriction fragments, one of which contained the nearly complete atpa- and atpb-gene. to complete the latter one, we sequenced a region of an overlapping fragment using synthetic oligonucleoti ... | 1995 | 7599166 |
| cloning of a carrot cdna for a member of the family of adp-ribosylation factors (arfs) and characterization of the binding of nucleotides by its product after expression in e. coli. | a cdna clone, designated dcarf1, was isolated from a cdna library prepared from embryogenic cell clusters of daucus carota and characterized. the cdna (883 bp) contained an open reading frame that encoded a protein of 181 amino acid residues with significant homology to the adp-ribosylation factors (arfs) of animals and yeast. the dcarf1-related transcripts were detected at a nearly constant level during somatic embryogenesis. the dcarf1-encoded protein was expressed as a fusion protein in e. co ... | 1995 | 7551586 |
| comparative study of the fermentative characteristics of inulin and different types of fibre in rats inoculated with a human whole faecal flora. | it is known that the physico-chemical characteristics of fibre modify their fermentation characteristics in the colon. previously we showed the varying effects of inulin and different types of fibre on the hepatic and intestinal xenobiotic-metabolizing enzymes (xme) in initially germ-free rats inoculated with a human, methanogenic, whole-faecal flora (roland et al. 1994). the aim of the present work was to assess whether or not these effects could be related to differences in production of ferme ... | 1995 | 7547841 |
| complementary immunolocalization patterns of cell wall hydroxyproline-rich glycoproteins studied with the use of antibodies directed against different carbohydrate epitopes. | antisera raised against the major hydroxyproline-rich glycoprotein (hrgp) in carrot (daucus carota l.) taproot, extensin-1, and a minor hrgp, extensin-2, were characterized by western blot analysis, enzyme-linked immunosorbent assay, and periodate oxidation and found to be directed against carbohydrate epitopes shared by both glycoproteins. the anti-extensin-1 antibodies (ge1) target periodate-sensitive epitopes and may recognize the terminal alpha-1,3-arabinoside of extensin-1. the anti-extensi ... | 1993 | 7506427 |
| hepatoprotective activity of carrot (daucus carota l.) against carbon tetrachloride intoxication in mouse liver. | the effect of carrot extract on carbon tetrachloride (ccl4)-induced acute liver damage was evaluated. the increased serum enzyme levels (viz., glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, lactate dehydrogenase, alkaline phosphatase, sorbitol and glutamate dehydrogenase) by ccl4-induction were significantly lowered due to pretreatment with the extract. the extract also decreased the elevated serum bilirubin and urea content due to ccl4 administration. increased activities ... | 1995 | 7500638 |
| analysis of the garden carrot (daucus carota l.) for linear furocoumarins (psoralens) at the sub parts per million level. | 2014 | 7096793 | |
| structure of t-dna in roots transformed by agrobacterium rhizogenes. | dna isolated from hairy roots incited on daucus carota by agrobacterium strains harboring the ri plasmid was probed with ri plasmid fragments to identify plasmid sequences transferred and integrated into the plant genome. one hairy root line was incited by an a. rhizogenes natural isolate strain harboring two large plasmids in addition to the ri plasmid. dna from hairy roots incited by this strain contained a total of five nonidentical copies of t-dna which together comprised a t-dna complement ... | 1983 | 6875427 |
| kinetics of golgi apparatus membrane flux following monensin treatment of embryogenic carrot cells. | ultrastructural changes resulting from treatment with the sodium selective ionophore, monensin, were studied in embryogenic suspension cultures of carrot, daucus carota (l.) in the presence of 10 microm monensin, an early change in the golgi apparatus was an increase in the number of cisternae per stack (dictyosome). an average of one additional cisterna per stack was formed within the first 2 to 4 min of monensin treatment; in some experiments a second cisterna was formed within about 8 min. th ... | 1983 | 6852059 |
| active polypeptide fragments common to prokaryotic, eukaryotic, and mitochondrial dna polymerases. | with a procedure that allows the renaturation of the dna polymerase catalytic activity in situ after sds-polyacrylamide gel electrophoresis, we have compared the active polypeptides present in extracts from organisms covering a wide evolutionary range from prokaryotes to eukaryotes, namely: escherichia coli, oryza sativa, daucus carota , neurospora crassa, dictyostelium discoideum, saccharomyces cerevisiae, ceratitis capitata, leucophaea maderae , xenopus laevis, rat tissues and human lymphoblas ... | 1982 | 6765191 |
| research on plants for fertility regulation in india. | this present review of indian plants investigated for fertility regulation includes published literature of the country and unpublished data of the central drug research institute (cdri), located in lucknow, india. publications without supportive experimental data have not been included. it is evident from the data presented in the tables that most of the investigators have failed to include the valuable information on the time and place of collection and proper botanical authentication, if co ... | 1982 | 6752588 |
| the effect of aldicarb on nematode population and its persistence in carrots, soil and hydroponic solution. | aldicarb, temik 15 g, was incorporated in furrows at 3.37 and 6.73 kg ai (active ingredient)/ha and carrots (daucus carota l.) were directly seeded on the same day. the numbers of nematode larvae were significantly suppressed in the treated plots; averages were 249, 74, and 51/50 cc soil samples for control (0), 3.37 and 6.73 kg ai/ha, respectively. aldicarb treatment resulted in a 28% yield increase as compared to the untreated. aldicarb residue in carrots was 28 ppb for the low treatment and 4 ... | 1984 | 6747220 |
| relative resistance of and delayed toxicity in undifferentiated plant cells to methyl mercury. | meristematic cells of carrot (daucus carota l., ca68-10) and lettuce (lactuca sativa l., le-67) cultured in 71-v medium (with 0.15 microgram/ml 2, 4-d) were given one initial dose (0.0001, 0.001, or 0.01 microgram/ml) of methyl mercury (mehg) at 0 hr, cultured for 288 hr. and then subcultured for 4 weeks. in another experiment, mehg was added in four daily doses (0.05, 0.10, 0.50, 1.0, 2.5, or 5.0 micrograms/ml) starting at 72 hr for 120 hr and then subcultured for 168 hr. the resulting 50% grow ... | 1984 | 6510322 |
| a link between transport and plasma membrane redox system(s) in carrot cells. | carrot (daucus carota l.) cells grown in suspension culture oxidized exogeneous nadh. the nadh oxidation was able to stimulate k+ (86rb+) transport into cells, but it did not affect sucrose transport. n,n'-dicyclohexyl-carbodiimide, diethylstilbestrol, and oligomycin, which only partially inhibited nadh oxidation, almost completely collapsed the k+ (86rb+) transport. vanadate, which is less effective as an ion transport inhibitor, was less effective in inhibiting the nadh-driven transport of k+ ... | 1984 | 6242153 |
| isolation and partial characterization of a substance from carrots, daucus carota, with ability to agglutinate cells of streptococcus mutans. | 1982 | 6184165 | |
| studies on daucus carota linn. ii. cholinergic activity of the quaternary base isolated from water-soluble fraction of alcoholic extract of seeds. | 1966 | 5979224 | |
| studies on daucus carota, linn. i. pharmacological studies with the water-soluble fraction of the alcoholic extract of the seeds: a preliminary report. | 1966 | 5934261 | |
| [the effect of ammonium ion on glucose metabolism in daucus carota cells]. | 1967 | 5618690 | |
| [formation of chloroplasts in tissue cultures of daucus carota in different hormonal conditions]. | 1967 | 5618688 | |
| [structure of the carotene crystals of daucus carota]. | 1971 | 5555933 | |
| kinetin-auxin ratio and development of chloroplasts in tissue cultures of daucus carota. | 1970 | 5444857 | |
| [demonstration of the nasturtium ringspot virus in the trumpet tree (catalpa bignonioides walt.) and carrots (daucus carota l.)]. | 1969 | 5396082 | |
| effect of chromatographic fractions of daucus carota linn. (seeds) on fertility in female albino rats. | 1972 | 5078109 | |
| monoamine oxidase inhibiting activity of daucus carota. | 1973 | 4783391 | |
| measurement of the five enzymes which convert chorismate to tryptophan in cultured daucus carota cell extracts. | 1973 | 4750744 | |
| anthranilate synthetase from 5-methyltryptophan-susceptible and -resistant cultured daucus carota cells. | 1972 | 4652566 | |
| tryptophan biosynthesis in nicotiana tabacum and daucus carota cell cultures: site of action of inhibitory tryptophan analogs. | 1972 | 4536831 | |
| anthiovulatory activity of five indigenous plants in rabbits. | the petroleum ether, alcoholic, and aqueous extracts of 5 indigenous plants, known to have antifertility activity in female rats and mice (areca catechu linn, carica papaya linn, daucus carota linn, mentha arvensis linn and polygonum hydropiper linn), were evaluated for their possible antiovulatory activity in rabbits with copper-induced ovulation. the alcoholic extract of mentha arvensis (leaves) and the petroleum ether extract of the roots of polygonum hydropiper were the most successful age ... | 1974 | 4442983 |