Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| in utero mycobacterium avium subspecies paratuberculosis infection of a pygmy goat. | 2009 | 19252216 | |
| evidence for age susceptibility of cattle to johne's disease. | calf rearing programs for the control of bovine johne's disease (bjd) in dairy farms have been widely adopted globally and are based on evidence that the most significant risk factor for developing the disease is exposure of young calves to infectious doses of the causative organism mycobacterium avium subsp. paratuberculosis (mptb). hygienic calf rearing practices aim to break the transmission cycle of mptb by removing neonatal calves from their dams within 12h of birth and segregating replacem ... | 2010 | 19246220 |
| genotype profiles of mycobacterium avium subspecies paratuberculosis isolates recovered from animals, commercial milk, and human beings in north india. | to understand the genetic diversity of mycobacterium avium subspecies paratuberculosis (map) isolates recovered from domestic and wild ruminants, commercial milk, and human beings in north india. | 2009 | 19233699 |
| development of a bovine ileal cannulation model to study the immune response and mechanisms of pathogenesis of paratuberculosis. | an ileal cannulation model was developed in conjunction with a flow cytometric assay to gain a better understanding of the mechanisms of immunopathogenesis of johne's disease caused by mycobacterium avium subsp. paratuberculosis. initial studies with calves showed that m. avium subsp. paratuberculosis dna is detectable by pcr in ileal biopsies during the first months following experimental infection. inflammatory lesions were not detected on endoscopic evaluation up to 8 months postexperimental ... | 2009 | 19225077 |
| the transport of mycobacterium avium subsp. paratuberculosis through saturated aquifer materials. | to investigate the processes controlling the transport of mycobacterium avium subsp. paratuberculosis (map) through aquifer materials. | 2009 | 19207858 |
| specific immunoassays confirm association of mycobacterium avium subsp. paratuberculosis with type-1 but not type-2 diabetes mellitus. | mycobacterium avium subspecies paratuberculosis (map) is a versatile pathogen with a broad host range. its association with type-1 diabetes mellitus (t1dm) has been recently proposed. rapid identification of infectious agents such as map in diabetic patients at the level of clinics might be helpful in deciphering the role of chronic bacterial infection in the development of autoimmune diseases such as t1dm. | 2009 | 19204799 |
| johne's disease in a free-ranging white-tailed deer from virginia and subsequent surveillance for mycobacterium avium subspecies paratuberculosis. | johne's disease (paratuberculosis) was diagnosed in a 2-yr-old, male, free-ranging white-tailed deer (odocoileus virginianus) from fauquier county, virginia, usa, based on histopathology and culture for mycobacterium avium subspecies paratuberculosis. clinical and pathologic findings included emaciation; loss of body fat; chronic diarrhea; severe, chronic, diffuse granulomatous colitis with intrahistiocytic acid-fast bacilli; moderate, chronic granulomatous lymphadenitis with intrahistiocytic ac ... | 2009 | 19204350 |
| coexpression of 16.8 kda antigen of mycobacterium avium paratuberculosis and murine gamma interferon in a bicistronic vector and studies on its potential as dna vaccine. | paratuberculosis or johne's disease is a chronic gastric disease of ruminants. for this disease there is no effective treatment or preventive measure available. 16.8 kda protein is an immunogenic protein of mycobacterium avium paratuberculosis and can be an ideal candidate for developing a dna vaccine construct. in present study a bicistronic dna vaccine construct pir16.8/ifn was developed using eukaryotic vector pires 6.1. two genes mpt (expressing 16.8 kda protein) and murine ifngamma were clo ... | 2009 | 19199070 |
| clinical trial on type of calving pen and the risk of disease in holstein calves during the first 90 d of life. | the objective of this study was to evaluate the efficacy of single cow calving pens that are cleaned between calvings vs. multiple cow calving pens for the prevention of calf diarrhea (scours), respiratory disease (pneumonia) and morbidity attributable to any cause. every other pregnant cow or heifer was moved to either the single cow calving pen (treatment) or the multiple cow calving pen (control) within 48-72 h prior to actual calving. the calves born in the single cow calving pens were assig ... | 2009 | 19193464 |
| evaluation of the immunogenicity of recombinant stress-associated proteins during mycobacterium avium subsp. paratuberculosis infection: implications for pathogenesis and diagnosis. | the aim of this study was to assess the immunogenicity of recombinant stress-associated proteins of mycobacterium avium subsp. paratuberculosis in sheep infected with the organism compared to control sheep. five proteins - map2411, clpp, ppa, map0593c and grea - which were identified previously in in vitro stress or dormancy responses of m. paratuberculosis to hypoxia, nutrient starvation and heat, were cloned, expressed and purified as his-tag recombinant proteins from the pet-15b vector in a b ... | 2009 | 19186011 |
| ovine small intestinal adenocarcinomas are not associated with infection by herpesviruses, helicobacter species or mycobacterium avium subspecies paratuberculosis. | sheep in new zealand more frequently develop small intestinal adenocarcinoma (sia) than sheep in other countries. the reasons for this high rate of intestinal neoplasia are not known. in man, differences between countries in the incidence of neoplasia are often due to differences in the rate of infection by carcinogenic viruses or bacteria. therefore, it was hypothesized that new zealand sheep more frequently develop sia as they are more frequently exposed to an infectious agent. this study comp ... | 2009 | 19159897 |
| mycobacterium avium subspecies paratuberculosis and its relationship with crohn's disease. | the hypothesis postulating that mycobacterium avium paratuberculosis (map) is the cause of crohn's disease (cd) has been circulating for many years. advances in molecular techniques, such as polymerase chain reaction and culture methods, have enabled researchers to demonstrate that there is an association between map and cd. recently, genome-wide association studies have identified novel susceptibility genes for cd, which are critical for generation of an adaptive immune response that is protect ... | 2009 | 19152445 |
| optical mapping of the mycobacterium avium subspecies paratuberculosis genome. | infection of cattle with mycobacterium avium subspecies paratuberculosis (m. ap) causes severe economic losses to the dairy industry in the usa and worldwide. in an effort to better examine diversity among m. ap strains, we used optical mapping to profile genomic variations between strains of m. ap k-10 (sequenced strain) and m. ap atcc 19698 (type strain). | 2009 | 19146697 |
| risk haplotype analysis for bovine paratuberculosis. | paratuberculosis (johne's disease), caused by mycobacterium avium subsp. paratuberculosis, is an important disease for bovines, although its genetic basis is poorly understood. in this study, three candidate genes were typed to study the associations between single nucleotide polymorphisms (snps) and paratuberculosis susceptibility (measured in a 1 or 0 form) at the haplotype level. a significant risk haplotype, constructed by a variant allele (c) at the first snp and a common allele (a) at the ... | 2009 | 19145459 |
| factors affecting isolation and identification of mycobacterium avium subsp. paratuberculosis from fecal and tissue samples in a liquid culture system. | culture of mycobacterium avium subsp. paratuberculosis is the definitive diagnostic test for johne's disease, a chronic granulomatous enteropathy of animals. compared to solid media, the identification of all strains of the organism in liquid media can be more difficult because the appearance of colonies and mycobactin dependence are not observable, and the growth of other organisms needs to be distinguished, commonly by pcr. factors affecting the isolation rate of s strains and the contaminatio ... | 2009 | 19144807 |
| comparison of three immunological diagnostic tests for the detection of avian tuberculosis in naturally infected red deer (cervus elaphus). | cases of tuberculosis due to mycobacterium avium subsp. avium in 52 adult red deer (cervus elaphus) from a farm were studied using different diagnostic techniques. immunological probes consisted of the comparative cervical tuberculin (cct) skin test, the interferon-gamma (ifn-gamma) assay, and 2 enzyme-linked immunosorbent assays (elisas) employing either avian purified protein derivatives or protoplasmatic antigen (ppa-3) as antigens. three of the animals were euthanized due to severe weakness, ... | 2009 | 19139508 |
| association between mycobacterium avium subsp. paratuberculosis infection among offspring and their dams in nondomestic ruminant species housed in a zoo. | the objective of the present study was to determine whether offspring of dams infected with mycobacterium avium subsp. paratuberculosis (map) have an increased risk for map infection. antemortem and postmortem disease surveillance data were used to identify positive and test-negative ruminants born at the zoological society of san diego (zssd) between 1991 and 2007 and to estimate cumulative lifetime incidence. a matched case-control study, nested within the population, was conducted and conditi ... | 2009 | 19139499 |
| pathogenesis of mycobacterium avium subsp. paratuberculosis in neonatal calves after oral or intraperitoneal experimental infection. | understanding the host response to mycobacterium avium subsp. paratuberculosis is critical to the development of effective vaccines and therapeutics for the control of this disease in the field. the current study compared the effectiveness of oral and intraperitoneal (ip) methods of experimental inoculation and two strains of m. avium subsp. paratuberculosis (strain k-10 and clinical isolate 509) on the level of infection and lesion development. calves were inoculated with 4x10(11) to 8x10(12)cf ... | 2009 | 19135813 |
| mycobacterium avium paratuberculosis invades human small-intestinal goblet cells and elicits inflammation. | crohn disease is a chronic inflammatory bowel disease of unknown etiology. mycobacterium avium paratuberculosis (map) was found in the gut of patients with crohn disease, but causality was not established. fully developed, germ-free human small intestine and colon were established by subcutaneous transplantation of fetal gut into scid (severe combined immunodeficiency) mice thereafter infected by direct intraluminal inoculation of map. we have found that map actively invades the human gut epithe ... | 2009 | 19133807 |
| toll-like receptors tlr1, tlr2 and tlr4 gene mutations and natural resistance to mycobacterium avium subsp. paratuberculosis infection in cattle. | toll like receptors (tlrs) are a class of pattern recognition receptors belonging to the innate immune system. mutations in the protein coding region of tlrs are associated with altered responsiveness to pathogen-associated molecular patterns (pamps). a search was performed for novel mutations in bovine tlr1, tlr2 and tlr4 genes associated with the mycobacterium avium subsp. paratuberculosis (map) infection. the work was also focused on the assessment of linkage between well known mutations in t ... | 2009 | 19131114 |
| oligonucleotide microarray technology and its application to mycobacterium avium subsp. paratuberculosis research: a review. | microarrays represent a modern powerful technology, which have potential applications in many areas of biological research and provide new insights into the genomics and transcriptomics of living systems. the aim of this review is to describe the application of microarray technology for mycobacterium avium subsp. paratuberculosis (map) research. the main focus points include a summary of results obtained for map using microarrays, examination of the fields of map research which are currently bei ... | 2009 | 19130317 |
| higher non-return rate associated with mycobacterium avium subspecies paratuberculosis infection at early stage in holstein dairy cows. | the effects of infection by mycobacterium avium paratuberculosis (map) on dairy cows are poorly documented and quite controversial. this retrospective study aimed at quantifying the variation in non-return to service of holstein dairy cows according to their map-infection status. three different statuses were defined based on both individual and herd tests results: elisa positive cow, all tests negative cow in a negative herd and all tests negative cow in a positive herd. whatever the age at map ... | 2009 | 19117602 |
| [antibiosis instead of the scalpel: new findings for crohn disease]. | 2009 | 19101890 | |
| development and validation of a triplex real-time pcr for rapid detection and specific identification of m. avium sub sp. paratuberculosis in faecal samples. | a triplex real-time (trt-pcr) assay was developed to ensure a rapid and reliable detection of mycobacterium avium subsp. paratuberculosis (map) in faecal samples and to allow routine detection of map in farmed livestock and wildlife species. the trt-pcr assay was designed using is900, ismap02 and f57 molecular targets. specificity of trt-pcr was first confirmed on a panel of control mycobacterial map and non-map strains and on faecal samples from map-negative cows (n=35) and from map-positive co ... | 2009 | 19095382 |
| real-time pcr, compared to liquid and solid culture media and elisa, for the detection of mycobacterium avium ssp. paratuberculosis. | a goal of johne's disease control programs is to accurately detect mycobacterium avium ssp. paratuberculosis (map) infected cattle as quickly as possible to reduce disease transmission. a newly introduced real-time pcr provides results rapidly, but its accuracy in the field has not been evaluated. fecal and serum samples collected from dairy cows in northern indiana were used to estimate the sensitivity and specificity of a newly licensed real-time pcr test for direct fecal detection of mycobact ... | 2009 | 19091493 |
| evaluation of progress made by dairy and beef herds enrolled in the minnesota johne's disease control program. | to evaluate progress made by cattle herds in the control of paratuberculosis (johne's disease) through participation in the minnesota johne's disease control program (mnjdcp). | 2008 | 19072610 |
| efficacy of novel lipid-formulated whole bacterial cell vaccines against mycobacterium avium subsp. paratuberculosis in sheep. | mycobacterium avium subsp. paratuberculosis [map], the causative agent of enteric johne's disease, incurs significant economic losses to the livestock industry. prophylactic vaccination can be employed as a control means, however mineral oil-based vaccines currently in practice have limited efficacy, produce strong antibody responses that confound serological diagnostic testing, and cause severe injection site reactions. in the present study, the safety and efficacy of a commercial mineral oil-a ... | 2009 | 19059295 |
| absence of mycobacterium avium subsp. paratuberculosis in crohn's patients. | mycobacterium avium subspecies paratuberculosis (map) has been suspected of involvement in crohn's disease (cd). we investigated this potential association by testing whole blood from cd patients and healthy controls for the presence of map by culture and molecular methods. in addition, each blood sample was analyzed for polymorphisms in the nod2/card15 gene previously associated with cd. | 2009 | 19058231 |
| discovery of stable and variable differences in the mycobacterium avium subsp. paratuberculosis type i, ii, and iii genomes by pan-genome microarray analysis. | mycobacterium avium subsp. paratuberculosis is an important animal pathogen widely disseminated in the environment that has also been associated with crohn's disease in humans. three m. avium subsp. paratuberculosis genomotypes are recognized, but genomic differences have not been fully described. to further investigate these potential differences, a 60-mer oligonucleotide microarray (designated the mapac array), based on the combined genomes of m. avium subsp. paratuberculosis (strain k-10) and ... | 2009 | 19047395 |
| colostrum and milk as risk factors for infection with mycobacterium avium subspecies paratuberculosis in dairy cattle. | mycobacterium avium ssp. paratuberculosis (map) infections cause major losses to the dairy industry. transmission of map occurs primarily via feces and in utero, but map can also be excreted in colostrum and milk. the objective of this study was to determine whether colostrum and milk fed to calves are important risk factors for infection with map. a questionnaire was sent to 1,050 farms participating in the danish control program on paratuberculosis in early 2007. details on practices regarding ... | 2008 | 19038936 |
| comparative analysis of mycobacterium avium subsp. paratuberculosis isolates from cattle, sheep and goats by short sequence repeat and pulsed-field gel electrophoresis typing. | mycobacterium avium subsp. paratuberculosis (map) causes the chronic enteritis called paratuberculosis mainly in cattle, sheep and goats. evidences that point out an association between map and crohn's disease in humans are increasing. strain differentiation among map isolates has proved to be difficult and has limited the study of the molecular epidemiology of paratuberculosis. in order to asses the usefulness of the pcr based short sequence repeat (ssr) analysis of locus 1 and locus 8 in the e ... | 2008 | 19032737 |
| insertion and deletion events that define the pathogen mycobacterium avium subsp. paratuberculosis. | mycobacterium avium comprises genetically related yet phenotypically distinct subspecies. consistent with their common origin, whole-genome sequence comparisons have revealed extensive synteny among m. avium organisms. however, the sequenced strains also display numerous regions of heterogeneity that likely contribute to the diversity of the individual subspecies. starting from a phylogenetic framework derived by multilocus sequence analysis, we examined the distribution of 25 large sequence pol ... | 2009 | 19028885 |
| association between milk antibody and interferon-gamma responses in cattle from mycobacterium avium subsp. paratuberculosis infected herds. | paratuberculosis is a chronic infection of ruminants caused by mycobacterium avium subsp. paratuberculosis (map). it is possible to detect infection with paratuberculosis at different stages of disease by means of various diagnostic test strategies. the objective of the present study was to evaluate if early cell-mediated immunity could predict the antibody results of milk samples in cattle with different faecal culture (fc) status. a group of 975 cows from 18 danish map infected dairy herds was ... | 2009 | 19027177 |
| mycobacterium avium subspecies paratuberculosis suppresses expression of il-12p40 and inos genes induced by signalling through cd40 in bovine monocyte-derived macrophages. | mycobacterium avium subspecies paratuberculosis (map) is a facultative intracellular organism that resides in host macrophages. map causes a fatal wasting syndrome in ruminants, typified by granulomatous enteritis in the small intestine. map has also been suspected as a causative or exacerbating factor in some cases of human crohn's disease. in map infections, a cytotoxic and proinflammatory th1-like response is essential to control disease. while such a response may initially develop, this typi ... | 2009 | 19022505 |
| analysis of the growth pattern, survival and proteome of mycobacteriumavium subsp. paratuberculosis following exposure to heat. | mycobacterium avium subsp. paratuberculosis (map) causes johne's disease in ruminants and may be involved in crohn's disease in humans. the aim of this study was to evaluate the in vitro growth pattern and proteome of map after heat stress following prior observations that map may exist in a dormant state in the environment when protected from extreme temperature flux and may survive pasteurization. data were obtained for two genomically distinct strains of map, sheep (s) and cattle (c), from 50 ... | 2009 | 19019575 |
| quantitative pcr monitoring of antibiotic resistance genes and bacterial pathogens in three european artificial groundwater recharge systems. | aquifer recharge presents advantages for integrated water management in the anthropic cycle, namely, advanced treatment of reclaimed water and additional dilution of pollutants due to mixing with natural groundwater. nevertheless, this practice represents a health and environmental hazard because of the presence of pathogenic microorganisms and chemical contaminants. to assess the quality of water extracted from recharged aquifers, the groundwater recharge systems in torreele, belgium, sabadell, ... | 2009 | 19011075 |
| advances in epidemiology and diagnosis of inflammatory bowel diseases. | the etiology of chronic inflammatory bowel diseases (ibd) remains unknown. understanding ibd epidemiology helps to identify at-risk individuals, to appreciate the changing demographic profile of ibd, and to interpret theories of ibd pathogenesis. this article reviews recent advances in epidemiology and diagnostics. the importance of clostridium difficile is highlighted. state-of-the-art ibd diagnostic modalities include serology, fecal markers, and advanced radiologic or endoscopic techniques. t ... | 2008 | 19006614 |
| presence of ppe proteins in mycobacterium avium subsp. paratuberculosis isolates and their immunogenicity in cattle. | johne's disease or paratuberculosis in cattle is caused by mycobacterium avium subsp. paratuberculosis (map). although the stages of infection have been well described, very few virulence factors of map have been studied in detail. we aimed to study the localization and immunogenicity of members of the polymorphic ppe protein family which is unique to mycobacteria and has been linked to virulence in mycobacterium tuberculosis (mtb). the presence of ppe proteins in the cell wall was investigated ... | 2009 | 19004580 |
| association between cow reproduction and calf growth traits and elisa scores for paratuberculosis in a multibreed herd of beef cattle. | the objective of this research was to assess the association between 4 cow reproductive and weight traits, and 2 preweaning calf traits and elisa scores for paratuberculosis (0 = negative, 1 = suspect, 2 = weak-positive, and 3 = positive) in a multibreed herd of cows ranging from 100% angus (a) to 100% brahman (b). cow data were 624 gestation lengths (gl), 358 records of time open (to), 605 calving intervals (ci), and 1240 weight changes from november to weaning in september (wc) from 502 purebr ... | 2009 | 18998232 |
| causality and gastrointestinal infections: koch, hill, and crohn's. | the gastrointestinal tract contains a complex mix of microorganisms. therefore, the finding of a particular microbe in the gastrointestinal tract or stools of a patient with intestinal disease does not necessarily indicate that the clinical state resulted from the presence of this organism. for this reason, extending from epidemiological association to causality is particularly challenging for gastrointestinal diseases. most established agents of acute bacterial gastroenteritis (eg, escherichia ... | 2008 | 18992408 |
| effect of paratuberculosis on the diagnosis of bovine tuberculosis in a cattle herd with a mixed infection using interferon-gamma detection assay. | interferon-gamma (ifn-gamma) detection assay is being applied as an ancillary test to tuberculin tests in the diagnosis of bovine tuberculosis to detect the maximum number of infected animals. among possible factors influencing the performance of tuberculosis-diagnostic tests, paratuberculosis, a widespread disease in spain and other european countries, has been pointed out as a cause of false positive reactions. still, its effect on the sensitivity of these tests in cattle has yet to be fully c ... | 2009 | 18986776 |
| test and teach. chronic soft tissue inflammation following accidental inoculation with a sheep vaccine. diagnosis: immune response to mineral oil and mycobacterium antigens in vaccine strain (gudair) of mycobacterium avium subsp.paratuberculosis. | 2008 | 18985526 | |
| review paper: modulation of mononuclear phagocyte function by mycobacterium avium subsp. paratuberculosis. | pathogenic mycobacteria are highly adapted for survival within host mononuclear phagocytes. this is largely due to the organism's capacity to prevent macrophage activation, block phagosome acidification and maturation, and attenuate presentation of antigens to the immune system. mycobacterium avium subsp. paratuberculosis (map) is one such organism that modulates the ruminant innate immune response. it is the causative agent in paratuberculosis, a chronic progressive granulomatous enteritis in r ... | 2008 | 18984786 |
| use of mycobacterium avium subsp. paratuberculosis specific coding sequences for serodiagnosis of bovine paratuberculosis. | in this study, the finished complete genome of mycobacterium avium subsp. paratuberculosis (map) was screened for specific coding sequences that could be very valuable in the design of a sensitive and specific map detection serological assay. eighty-seven map-specific sequences were retained. among these, three candidate antigens have been analysed for their serodiagnostic potential. these antigens were selected on the basis of their putative immunogenicity as predicted by in silico analysis. th ... | 2009 | 18977614 |
| evaluation of immune responses and protective efficacy in a goat model following immunization with a coctail of recombinant antigens and a polyprotein of mycobacterium avium subsp. paratuberculosis. | the protective efficacy of four recombinant antigens (85a, 85b, superoxide dismutase [sod], and a fusion polypeptide [map74f]) of mycobacterium avium subsp. paratuberculosis (map) along with the adjuvant dimethydioctadecyl ammonium bromide (dda) was assessed in a goat challenge model. animals were immunized with the four antigens with adjuvant dda (group i, eight goat kids) or without the adjuvant (group ii, eight goat kids) or adjuvant only (group iii, nine goat kids). animals were boostered 3 ... | 2009 | 18955101 |
| agreement between elisa and complement fixation test used for diagnosing of paratuberculosis in goats. | an elisa with a lipoarabinomannan as an antigen, developed for diagnosis of bovine paratuberculosis, has been adapted for use in goats, and compared with complement fixation test. kappa value of 0.62 indicated good agreement between cft and the adapted elisa and proved that the investigated elisa may be helpful in diagnosis of mycobacterium avium subsp. paratuberculosis infection in goats. the elisa has been used to screen a randomly selected representative sample of polish breeding goat populat ... | 2008 | 18942542 |
| association between card15/nod2 gene polymorphisms and paratuberculosis infection in cattle. | paratuberculosis represents a major problem in farmed ruminants and at the present is considered a potential zoonosis. the disease is caused by mycobacterium avium subsp. paratuberculosis, and susceptibility to infection is suspected to have a genetic component. caspase recruitment domain 15 (card15) gene encodes for a cytosolic protein implicated in bacterial recognition during innate immunity. crohn's disease (cd) is an idiopathic inflammatory bowel disease in humans comparable in many feature ... | 2009 | 18926647 |
| mycobacterium avium subspecies induce differential expression of pro-inflammatory mediators in a murine macrophage model: evidence for enhanced pathogenicity of mycobacterium avium subspecies paratuberculosis. | mycobacterium avium subspecies (ssp.) paratuberculosis (map) is the etiological agent of paratuberculosis, a chronic, non-treatable granulomatous enteritis of ruminants. map is the only mycobacterium affecting the intestinal tract, which is of interest since it is presently the most favoured pathogen linked to crohn's disease (cd) in humans due to its frequent detection in cd tissues. map is genetically closely related to other m. avium ssp. such as m. avium ssp. avium (maa) and m. avium ssp. ho ... | 2008 | 18926302 |
| combined multilocus short-sequence-repeat and mycobacterial interspersed repetitive unit-variable-number tandem-repeat typing of mycobacterium avium subsp. paratuberculosis isolates. | short-sequence-repeat (ssr) sequencing was applied to 127 mycobacterium avium subsp. paratuberculosis isolates typed by mycobacterial interspersed repetitive unit-variable-number tandem repeats (miru-vntr) and is900 restriction fragment length polymorphism (rflp). combined miru-vntr and ssr typing followed by secondary is900 rflp typing is an improved approach to high-resolution genotyping of this pathogen. | 2008 | 18923016 |
| association between mycobacterium avium subsp. paratuberculosis dna in blood and cellular and humoral immune response in inflammatory bowel disease patients and controls. | similarities between human inflammatory bowel disease (ibd) and ruminant paratuberculosis have fueled a heated discussion on the role of mycobacterium avium subsp. paratuberculosis (map) in the etiology of ibd. | 2009 | 18922720 |
| decrease of johne's disease prevalence and incidence in six minnesota, usa, dairy cattle herds on a long-term management program. | the objective of this prospective longitudinal field study was to describe changes in prevalence of seroconversion and fecal shedding and changes in incidence rate of seroconversion, fecal shedding and culling of milk cows for clinical signs of johne's disease (jd) in six minnesota (usa) herds participating to the jd demonstration herd project (jddhp) from 2000 to december 2005. changes in prevalence and incidence rate were evaluated in light of the owner's compliance to the jddhp using a risk a ... | 2009 | 18848365 |
| immunogenicity of proteome-determined mycobacterium avium subsp. paratuberculosis-specific proteins in sheep with paratuberculosis. | mycobacterium avium subsp. paratuberculosis causes paratuberculosis, a chronic granulomatous enteritis. detecting animals with paratuberculosis infections is difficult because the currently available tools have low sensitivity and lack specificity; these tools are prone to generating spurious positive test results caused by exposure to environmental m. avium complex organisms. to generate candidate antigens for incorporation into a specific test for paratuberculosis, subspecies-specific proteins ... | 2008 | 18845834 |
| genomic analysis of local isolate of mycobacterium avium subspecies paratuberculosis. | mycobacterium avium subspecies paratuberculosis (map) causes johne's disease (jd or paratuberculosis) in animals and has also been implicated in crohn's disease of humans. it has been shown that map is endemic in animal population of india. understanding of heterogeneity among map strains is important both for diagnosis and design control measures. genotyping and epidemiological investigations revealed that map 'bison type' was the predominant strain infecting domestic ruminant population in ind ... | 2009 | 18838235 |
| comparison between two in situ methods for mycobacterium avium subsp. paratuberculosis detection in tissue samples from infected cattle. | paratuberculosis or johne's disease is a chronic infectious disorder caused by mycobacterium avium subsp. paratuberculosis (map). the disease produces diarrhea and weight loss in cattle and other animal species, and it is characterized by granulomatous enteritis and lymphadenitis. histopathology and in situ techniques can be used as a diagnostic test, but the performance of these methods was not previously compared. the aim of this paper was to evaluate the ability of immunohistochemistry and in ... | 2009 | 18838233 |
| real-time pcr identification of mycobacterium avium subspecies paratuberculosis in ovine and bovine tissues. | 2008 | 18836157 | |
| diagnostic detection methods for mycobacterium avium subsp. paratuberculosis in white-tailed deer. | this study compares the results and suitability of serological testing, microscopic examination, deoxyribonucleic acid (dna) detection, and bacterial culture for detecting mycobacterium avium subsp. paratuberculosis (map) infection in asymptomatic farmed white-tailed deer (wtd) (odocoileus virginianus). deer were classified as infected if culture slants from their feces, lymph nodes, or ileum were positive, or if a polymerase chain reaction (pcr) assay detected map dna in any of its tissues. dee ... | 2008 | 18827845 |
| financial modelling of the potential cost of ovine johne's disease and the benefit of vaccinating sheep flocks in southern new south wales. | to develop an enterprise gross margin (gm) model that predicts the on-farm financial impact of ovine johne's disease (ojd) for various sheep enterprises in australia. in addition, to estimate the benefits and costs of control through the use of the gudair vaccination, including a breakeven point. | 2008 | 18826512 |
| on-farm spread of mycobacterium avium subsp. paratuberculosis in raw milk studied by is900 and f57 competitive real time quantitative pcr and culture examination. | a rapid, cheap and sensitive detection method of mycobacterium avium subsp. paratuberculosis (map) in raw milk was needed for routine usage. we developed two duplex real time qpcr systems specific for map detection. these real time qpcr assays amplify the multicopy element is900 for qualitative analysis and the single copy element f57 for quantitative analysis. both assays incorporate an internal amplification control amplified with the same primers as the targets and the same probes are used in ... | 2008 | 18824269 |
| a review of prevalences of paratuberculosis in farmed animals in europe. | prevalence estimates are used by decision makers such as policy makers and risk assessors to make choices related to certain diseases and infections. paratuberculosis, caused by mycobacterium avium subsp. paratuberculosis (map), is a chronic infection particularly resulting in economic losses among farmed ruminants. therefore, this infection is of concern for many farming industry decision makers. as a result, multiple studies have been carried out to determine the within-herd and between-herd p ... | 2009 | 18817995 |
| is mycobacterium paratuberculosis or any other infectious agent involved in the pathogenesis of ibd? | 2008 | 18816732 | |
| evaluation of four methods of dna recovery from mycobacterium avium subspecies paratuberculosis present in intestine tissue of goats and comparative sensitivity of is900 pcr with respect to culture for diagnosis of johne's disease. | low sensitivity of pcr reaction for detection of mycoobacterium avium subspecies paratuberculosis (map) in tissues and fecal samples is mainly attributed to false negative results. present study was undertaken to compare four methods of dna isolation from tissues of infected animals and to determine most sensitive protocol for the recovery of dna, suitable for is900 pcr based detection of johne's disease infection. method i, the traditional van soolingen2 method of dna isolation was adopted for ... | 2008 | 18814486 |
| wildlife (boselaphus tragocamelus)-small ruminant (goat and sheep) interface in the transmission of 'bison type' genotype of mycobacterium avium subspecies paratuberculosis in india. | information on mycobacterium avium subspecies paratuberculosis (map) genotypes infecting different animal species in india is limited. presence of map was investigated in free ranging antelopes (locally known as nilgai/blue bulls/boselaphus tragocamelus) using direct microscopy, culture, is900 pcr and is1311 pcr-rea. is900 elements of map from nilgai and previously isolated from goats were sequenced and compared to establish inter-species transmission between free ranging nilgai and closed farm ... | 2010 | 18805585 |
| prevalence of avian influenza viruses, borrelia garinii, mycobacterium avium, and mycobacterium avium subsp. paratuberculosis in waterfowl and terrestrial birds in slovakia, 2006. | the prevalence of borrelia, mycobacteria and avian influenza virus (aiv) infections, together with the distribution of different aiv subtypes, was studied in migratory waterfowl and terrestrial birds trapped in three localities in slovakia during 2006. samples obtained from waterfowl captured in the senianske ponds area of eastern slovakia showed the highest diversity of aiv isolates. a total of 13 different subtypes were detected in 19 samples from this location (h1n2, h2n2, h3n2, h6n6, h7n6, h ... | 2008 | 18798030 |
| strain-specific sensitivity estimates of mycobacterium avium subsp. paratuberculosis culture in greek sheep and goats. | the requirements for the isolation of mycobacterium avium subsp. paratuberculosis (map) may be related to the strain-type [sheep (s)- or cattle (c)-type] and not to the host. the objective of this paper was to estimate and compare strain- and biological sample (faeces or pooled-tissue)--specific sensitivities (ses) of two solid culture media, herrold's egg yolk medium (heym) and lowenstein-jensen (lj) medium, for the isolation of map from greek dairy sheep and goats. from 400 faecal samples coll ... | 2009 | 18793275 |
| cows, crohn's and more: is mycobacterium paratuberculosis a superantigen? | the plausibility of a causal role of mycobacterium avium subspecies paratuberculosis (map) in crohn's disease has gone from controversial to compelling. this century old debate is resolving because of unfolding understanding of shared genetic susceptibilities for crohn's and mycobacterial infection in addition to newer laboratory tests to detect map which have linked map and crohn's. mycobacterial heat shock proteins are associated with a multitude of "autoimmune" diseases, including type 1 diab ... | 2008 | 18789596 |
| growth pattern and partial proteome of mycobacterium avium subsp. paratuberculosis during the stress response to hypoxia and nutrient starvation. | mycobacterium avium subsp. paratuberculosis is an important pathogen that causes johne's disease in animals and has been implicated in crohn's disease in man yet few data exist on its physiological adaptation in either the host or the environment. in this study, the proteomic responses of the two distinct strains of m. a. paratuberculosis, cattle (c) and sheep (s), to hypoxia and starvation were studied in vitro. nutrient starvation inhibited growth of both strains and was lethal for s strain af ... | 2009 | 18786786 |
| pasteurization of colostrum reduces the incidence of paratuberculosis in neonatal dairy calves. | in the present study, the potential benefits of feeding pasteurized colostrum were demonstrated in calves born to dams naturally infected with mycobacterium avium ssp. paratuberculosis. calves were separated at birth from their dams and randomly allocated into a group fed either the colostrum of their dam (dc; n = 6), followed by feeding the milk of the dam for 3 wk and then milk replacer, or into a group fed pooled pasteurized colostrum (pc; n = 5) from healthy noninfected dams, followed by mil ... | 2008 | 18765618 |
| risk factors for mycobacterium avium subspecies paratuberculosis in fars province (southern iran) dairy herds. | a cross-sectional study was conducted from march to august 2006 in dairy herds in fars province, southern iran to determine the herd-level risk factors for infection with mycobacterium avium subspecies paratuberculosis (map). statistical analysis using multivariable logistic regression showed that contamination of udders of periparturient cows with manure (or = 6.4, p = 0.02) and history of having suspected cases of johne's disease in the herd (or = 6.7, p = 0.04) were significantly associated w ... | 2009 | 18726705 |
| abnormalities in the handling of intracellular bacteria in crohn's disease: a link between infectious etiology and host genetic susceptibility. | the etiology of crohn's disease (cd) is still poorly understood, but recent advances have highlighted the importance of the innate immune system and the critical relationship between the gut flora and the intestinal mucosa. several combinations of genetic factors predisposing to cd have been described, with the most significant replicable associations including genes for intracellular receptors of bacterial cell walls (nod2/card15) and for bacterial clearance and antigen processing via autophagy ... | 2008 | 18726145 |
| a robust method for bacterial lysis and dna purification to be used with real-time pcr for detection of mycobacterium avium subsp. paratuberculosis in milk. | a possible mode of transmission for the ruminant pathogen mycobacterium avium subsp. paratuberculosis (map) from cattle to humans is via milk and dairy products. although controversially, map has been suggested as the causative agent of crohn's disease and its presence in consumers' milk might be of concern. a method to detect map in milk with real-time pcr was developed for screening of bulk tank milk. pellet and cream fractions of milk were pooled and subjected to enzymatic digestion and mecha ... | 2008 | 18694788 |
| risk factors associated with mycobacterium avium subspecies paratuberculosis seropositivity in canadian dairy cows and herds. | our objective was to determine the risk factors associated with the seroprevalence of mycobacterium avium subspecies paratuberculosis (map) in a large number of randomly selected canadian dairy herds, controlling for important confounding variables and co-infections with bovine leukemia virus (blv), bovine viral diarrhea virus (bvdv) and neospora caninum (nc). serum samples from 30 randomly selected cows, where available, in 315 herds from seven provinces were tested for antibodies against blv, ... | 2009 | 18692926 |
| differential expression of cd5 on b lymphocytes in cattle infected with mycobacterium avium subsp. paratuberculosis. | cd5 is a cell surface molecule involved in antigen recognition and is present on all t lymphocytes and a subset of b lymphocytes. the purpose of this study was to examine cd5+ expression on peripheral blood b cells from healthy, noninfected cattle and cattle with subclinical and clinical paratuberculosis. peripheral blood mononuclear cells (pbmc) were freshly isolated or cultured for 7 days in the presence or absence of live mycobacterium avium subsp. paratuberculosis (m. avium subsp. paratuberc ... | 2008 | 18692906 |
| transcriptional analysis of diverse strains mycobacterium avium subspecies paratuberculosis in primary bovine monocyte derived macrophages. | in this study we analyzed the macrophage-induced gene expression of three diverse genotypes of mycobacterium avium subsp. paratuberculosis (map). using selective capture of transcribed sequences (scots) on three genotypically diverse map isolates from cattle, human, and sheep exposed to primary bovine monocyte derived macrophages for 48 h and 120 h we created and sequenced six cdna libraries. sequence annotations revealed that the cattle isolate up-regulated 27 and 241 genes; the human isolate u ... | 2008 | 18692151 |
| distribution of mycobacterium avium subspecies paratuberculosis in the lower florida keys. | johne's disease, a fatal and contagious gastrointestinal infection caused by mycobacterium avium subsp. paratuberculosis (map), was first diagnosed in an endangered florida key deer (odocoileus virginianus clavium) in 1996 and later in six additional key deer deaths from 1998 to 2004. we investigated the geographic distribution of map in the lower florida keys from february 2005 through may 2006 via collection of blood and fecal pellets from 51 live-captured deer, collection of 550 fecal samples ... | 2008 | 18689642 |
| isolation of mycobacterium avium subsp. paratuberculosis from waste milk delivered to california calf ranches. | the objective of this study was to determine if viable mycobacterium avium subsp. paratuberculosis (map) was present in waste milk delivered and fed to calves on california calf ranches. four calf-raising facilities in the central valley of california that fed pasteurized waste milk to calves were enrolled. pre- and post-pasteurization waste milk samples were cultured for map using liquid and solid media over a 5-day period during each of four seasons. aerobic cultures were performed simultaneou ... | 2008 | 18681799 |
| antibacterial activities of naturally occurring compounds against mycobacterium avium subsp. paratuberculosis. | the antibacterial activities of 18 naturally occurring compounds (including essential oils and some of their isolated constituents, apple and green tea polyphenols, and other plant extracts) against three strains of mycobacterium avium subsp. paratuberculosis (a bovine isolate [nctc 8578], a raw-milk isolate [806r], and a human isolate [atcc 43015]) were evaluated using a macrobroth susceptibility testing method. m. avium subsp. paratuberculosis was grown in 4 ml middlebrook 7h9 broth containing ... | 2008 | 18676709 |
| crystal structure of a udp-glucose-specific glycosyltransferase from a mycobacterium species. | glycosyltransferases (gts) are a large and ubiquitous family of enzymes that specifically transfer sugar moieties to a range of substrates. mycobacterium tuberculosis contains a large number of gts, many of which are implicated in cell wall synthesis, yet the majority of these gts remain poorly characterized. here, we report the high resolution crystal structures of an essential gt (map2569c) from mycobacterium avium subsp. paratuberculosis (a close homologue of rv1208 from m. tuberculosis) in i ... | 2008 | 18667419 |
| vaccination against paratuberculosis. | johne's disease, or paratuberculosis, is a chronic granulomatous enteritis in ruminants caused by mycobacterium avium subsp. paratuberculosis (map) affecting principally cattle, sheep and goats. primarily, there are two clinical signs: cachexia and chronic diarrhea (less common in goats and sheep). this disease results in considerable economic losses in livestock industry, particularly the dairy sector. the route of transmission is mostly by the fecal-oral route, but hygienic measures and cullin ... | 2008 | 18665779 |
| immunology of mycobacterial infections: with special reference to mycobacterium avium subspecies paratuberculosis. | the interplay between mycobacteria and host determines the outcome of infection. after uptake of mycobacteria by macrophages, several possible scenarios may emerge; mycobacteria may be destroyed immediately or there is establishment of persistent infection. this review is focused around mycobacteria-host interactions with reference to mycobacterium avium subspecies paratuberculosis (map) infection and highlights protective mechanisms involved in order to design vaccines and other control strateg ... | 2008 | 18656706 |
| immunogenicity and protective efficacy of dna vaccines encoding map0586c and map4308c of mycobacterium avium subsp. paratuberculosis secretome. | mycobacterium avium subsp. paratuberculosis (map), the etiological agent of chronic enteritis of the small intestine in domestic and wild ruminants, causes substantial losses to livestock industry. control of this disease is seriously hampered by the lack of adequate diagnostic tools, vaccines and therapies. in this study, we have evaluated the vaccine potential of two map proteins, i.e. map0586c and map4308c, previously identified by postgenomic and immunoproteomic analysis of map secretome as ... | 2008 | 18652866 |
| scenario analysis of changes in consumption of dairy products caused by a hypothetical causal link between mycobacterium avium subspecies paratuberculosis and crohn's disease. | johne's disease is an infectious disease of cattle caused by mycobacterium avium subspecies paratuberculosis (map). crohn's disease (cd) is a human disease of unknown etiology that is characterized by chronic bowel inflammation. no causal link has been scientifically established between map and cd, but it is important to understand possible impacts on society should such a causal link be established. the goal of this paper is to analyze the implications and the possible economic impacts that fin ... | 2008 | 18650302 |
| heritability estimates for antibody response to mycobacterium avium subspecies paratuberculosis in german holstein cattle. | the objective of this study was to estimate heritability of antibody response to mycobacterium avium ssp. paratuberculosis (map) in 4,524 german holstein cows by applying linear and threshold models. data were collected within a paratuberculosis voluntary control program in thuringia, germany, in 2005. the map-positive prevalence of the 12 farms in the data set varied between 5 and 36.9%. a nearly linear increase in prevalence was observed from 2- to 3-yr-old cows, whereas prevalence declined in ... | 2008 | 18650301 |
| osteopontin: a novel cytokine involved in the regulation of mycobacterium avium subspecies paratuberculosis infection in periparturient dairy cattle. | osteopontin (opn), an important mediator of the cell-mediated immune response, enhances the host immune response against mycobacterial infections. infections caused by mycobacterium avium ssp. paratuberculosis (map) have a devastating effect on the dairy industry. we sought to characterize opn at the level of gene and protein expression in periparturient dairy cows naturally infected with map. peripheral blood mononuclear cells (pbmc) were isolated from control, subclinical, and clinical peripar ... | 2008 | 18650284 |
| detection of mycobacterium avium subspecies paratuberculosis-specific dna by pcr in intestinal biopsies of dogs. | mycobacterium avium subspecies paratuberculosis (map) is the cause of paratuberculosis. map infections have not been reliably detected in dogs, but a reemerging debate about the link between map and crohn's disease has renewed interest about the occurrence of map in pets. | 2008 | 18638019 |
| estimate of the direct production losses in canadian dairy herds with subclinical mycobacterium avium subspecies paratuberculosis infection. | the objective of this study was to estimate the annual losses from mycobacterium avium subspecies paratuberculosis (map) for an average, map-seropositive, canadian dairy herd. a partial-budget simulation model was developed with 4 components of direct production losses (decreased milk production, premature voluntary culling, mortality, and reproductive losses). input values were obtained primarily from a national seroprevalence survey of 373 canadian dairy farms in 8 of 10 provinces. the model t ... | 2008 | 18624066 |
| pathogenic agents in inflammatory bowel diseases. | infectious agents are still thought to be involved in the origin of inflammatory bowel disease. the focus in recent years has been more on mycobacterium avium subsp paratuberculosis, adherent-invasive escherichia coli or yeasts. | 2008 | 18622157 |
| osteopontin immunoreactivity in the ileum and ileocecal lymph node of dairy cows naturally infected with mycobacterium avium subsp. paratuberculosis. | osteopontin (opn), a highly acidic glycoprotein, promotes cellular adhesion and recruitment and has been shown to be upregulated in the granulomas of mycobacterial infections. johne's disease, caused by mycobacterium avium subsp. paratuberculosis (map), is associated with granulomatous enteritis. the objective of this experiment was to identify opn in the ileum and ileocecal lymph node (icn) of dairy cows naturally infected with map and to compare the frequency and intensity of staining between ... | 2008 | 18620757 |
| radiometric pooled faecal culture for the detection of mycobacterium avium subsp paratuberculosis in low-shedder cattle. | to identify the optimum pooling rate for pooled faecal culture (pfc) as a diagnostic tool in bovine johne's disease control, for detection of cattle shedding low concentrations of mycobacterium avium subsp paratuberculosis (map). | 2008 | 18616475 |
| mycobacterium avium subspecies paratuberculosis and crohn's disease granulomas. | chronic infection with mycobacterium avium subspecies paratuberculosis (m. paratuberculosis) has been proposed as a cause of crohn's disease. although numerous investigators have examined the link between m. paratuberculosis and crohn's disease, the evidence remains controversial. the aim of this study was to examine intestinal granuloma from crohn's patients for m. paratuberculosis using a semi-nested m. paratuberculosis-specific is900 polymerase chain reaction (pcr). | 2008 | 18609156 |
| antigen-specific regulatory t cells in bovine paratuberculosis. | mycobacterium avium subspecies paratuberculosis (map) is an intracellular pathogen that survives in the host's intestinal macrophages and causes chronic enteritis in ruminants. the subclinical stage of map infection is accompanied by loss of pro-inflammatory t(h)1 response, and a predominant, but ineffective, antibody-mediated t(h)2 response. how map interacts with the bovine immune system and suppresses t(h)1 responses is unclear. studies carried out in our lab and others indicate that when per ... | 2008 | 18602164 |
| transitions in diagnostic tests used for detection of mycobacterium avium subsp. paratuberculosis infections in cattle. | diagnosis of infections with mycobacterium avium subsp. paratuberculosis (map) is difficult due to a long incubation period and lack of tests which can accurately predict the future status of animals. early detection of infectious animals is necessary to reduce transmission of map. the objective of this study was to determine the time from first detection of map-antibodies in milk elisa to start of map shedding, for animals with various shedding patterns. an observational longitudinal study was ... | 2008 | 18599225 |
| on the prevalence of m. avium subspecies paratuberculosis dna in the blood of healthy individuals and patients with inflammatory bowel disease. | mycobacteria, such as m. leprae and m. tuberculosis infect billions of humans. however, because of appropriate immune responses and antibiotic therapy, overt mycobacterial diseases occur far less frequently. m. avium subspecies paratuberculosis (map) causes johne's disease in ruminants, an affliction evocative of inflammatory bowel disease (ibd). several agents used to treat ibd (5-asa, methotrexate, azathioprine and its metabolite 6-mp) have recently been shown to be antimap antibiotics. we her ... | 2008 | 18596984 |
| herd characteristics and management practices associated with seroprevalence of mycobacterium avium subsp paratuberculosis infection in dairy herds. | to investigate herd characteristics and management practices associated with a high seroprevalence of mycobacterium avium subsp paratuberculosis (map) in dairy herds in central california. | 2008 | 18593244 |
| immunization with a dna vaccine cocktail induces a th1 response and protects mice against mycobacterium avium subsp. paratuberculosis challenge. | several antigens of mycobacterium avium subsp. paratuberculosis have been studied as vaccine components and their immunogenicity has been evaluated. previously, we reported that 85 antigen complex (85a, 85b, and 85c), superoxide dismutase (sod), and 35kda protein could induce significant lymphocyte proliferation as well as the elaboration of th1-associated cytokines including interferon gamma (ifn-gamma), interleukin-2 (il-2), il-12 and tumor necrosis factor alpha (tnf-alpha). based on these res ... | 2008 | 18582521 |
| the importance of culling in johne's disease control. | johne's disease is caused by mycobacterium avium subsp. paratuberculosis (map) infection and results in economic losses in the dairy industry. to control map transmission in herds, test-based culling has been recommended and immediate culling of high shedding animals is typically implemented. in this study, we quantified the effects of map control in us dairy herds, using the basic reproduction ratio r(0). the effectiveness of culling strategies was evaluated for good and poor herd management (l ... | 2008 | 18573505 |
| detection of mycobacterium avium subspecies paratuberculosis: comparing fecal culture versus serum enzyme-linked immunosorbent assay and direct fecal polymerase chain reaction. | mycobacterium avium ssp. paratuberculosis (map) is the etiologic agent of johne's disease in cattle. the disease causes diarrhea, reduced milk production, poor reproductivity, emaciation, and eventually death. culture on herrold's egg yolk agar is considered to be the definitive test for diagnosis of johne's in cattle. this method has moderate sensitivity (30 to 50%) and is 100% specific; however, it can take up to 16 wk due to the slow growth of map. currently, serum elisa is used to screen her ... | 2008 | 18565921 |
| evaluation of five elisa test kits for the measurement of antibodies against mycobacterium avium subspecies paratuberculosis in bovine serum. | five commercially available elisa tests for the detection of antibodies against mycobacterium avium subsp. paratuberculosis in bovine serum were evaluated at the individual animal level using sera from 286 paratuberculosis-free and 110 paratuberculosis-infected dairy cattle. sensitivity (se) and specificity (sp) of the tests were estimated after determination of the cut-off dtheta by tg-roc analysis or using the cut-off values recommended by the manufacturers, respectively. when the dtheta cut-o ... | 2008 | 18557524 |
| diagnosis of bovine paratuberculosis by a novel enzyme-linked immunosorbent assay based on early secreted antigens of mycobacterium avium subsp. paratuberculosis. | we previously reported that protein antigens of serodiagnostic potential were more abundant in culture filtrates than cellular extracts from liquid cultures of mycobacterium avium subsp. paratuberculosis (d. cho and m. t. collins, clin. vaccine immunol. 13:1155-1161, 2006). based on this observation, a novel enzyme-linked immunosorbent assay (elisa) using antigens secreted by young (early- to mid-log-phase) cultures of m. avium subsp. paratuberculosis jtc303 (a low-passage isolate originating fr ... | 2008 | 18550730 |
| the recovery of mycobacterium avium subspecies paratuberculosis from the intestine of infected ruminants for proteomic evaluation. | johne's disease is a slowly developing intestinal disease, primarily of ruminants, caused by mycobacterium avium subspecies paratuberculosis. the disease contributes to significant economic losses worldwide in agricultural industry. analysis of bacterial proteomes isolated directly from infected animals can provide important information about the repertoire of proteins present during infection and disease progression. in this study, m. avium subspecies paratuberculosis has been extracted from jo ... | 2008 | 18547663 |
| effectiveness of mycobacterium paratuberculosis isolation from raw milk by means of direct isolation of dna and classic culture. | detection of mycobacterium paratuberculosis (map) in tissues of patients suffering from crohn's disease has given rise to speculation that this mycobacterium may play some role in the development of this disease in humans. food products, especially milk obtained from animals infected with paratuberculosis, may be a potential vector of map to humans, yet the detection of this pathogen poses a number of difficulties. this study was aimed at comparing the effectiveness of map isolation from milk sa ... | 2008 | 18540204 |