Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| safety, efficacy and utility of methods of transferring adhesive and cohesive escherichia coli cells to microplates to avoid aerosols. | the virtual colony count (vcc) microbiological assay has been utilized for over a decade to measure the antimicrobial activity of peptides such as defensins and ll-37 against biosafety level (bsl)-1 and bsl-2 bacteria including escherichia coli, staphylococcus aureus, bacillus cereus, and enterobacter aerogenes. in addition, a modified pipetting technique was presented in a 2011 study of defensin activity against the bsl-3 pathogen bacillus anthracis. both studies were published in the journal ... | 2014 | 25671086 |
| the dipeptidyl peptidase iv inhibitors vildagliptin and k-579 inhibit a phospholipase c: a case of promiscuous scaffolds in proteins. | the long term side effects of any newly introduced drug is a subject of intense research, and often raging controversies. one such example is the dipeptidyl peptidase-iv (dpp4) inhibitor used for treating type 2 diabetes, which is inconclusively implicated in increased susceptibility to acute pancreatitis. previously, based on a computational analysis of the spatial and electrostatic properties of active site residues, we have demonstrated that phosphoinositide-specific phospholipase c (pi-plc) ... | 2013 | 25671081 |
| purification and identification of an antibacterial protein from the symbiotic bacteria associated with novel entomopathogenic nematode, rhabditis (oscheius) sp. | entomopathogenic nematodes (epn) belonging to the families steinernematidae and heterorhabditidae and their symbiotic bacteria xenorhabdus and photorhabdus are well-known as biological control agents and are found to produce a wide range of bioactive secondary metabolites. studies carried out at the central tuber crops research institute (ctcri) on entomopathogenic nematodes resulted in the identification of novel epn belonging to the family rhabditidae. this study reports the purification of a ... | 2015 | 25666178 |
| chemodiversity of cereulide, the emetic toxin of bacillus cereus. | food-borne intoxications are increasingly caused by the dodecadepsipeptide cereulide, the emetic toxin produced by bacillus cereus. as such intoxications pose a health risk to humans, a more detailed understanding on the chemodiversity of this toxin is mandatory for the reliable risk assessment of b. cereus toxins in foods. mass spectrometric screening now shows a series of at least 18 cereulide variants, among which the previously unknown isocereulides a-g were determined for the first time by ... | 2015 | 25665710 |
| pathogenicity and characterization of a novel bacillus cereus sensu lato isolate toxic to the mediterranean fruit fly ceratitis capitata wied. | the lethal and sub-lethal effects of sporulated cultures of a novel bacillus cereus sensu lato strain lacking detectable cry genes and identified through morphological and genetic analyses, have been studied on the mediterranean fruit fly ceratitis capitata. the lethal effects on young larvae were concentration dependent, with a median lethal concentration (lc50) of 4.48 × 10(8)spores/g of diet. sporulated cultures of this strain significantly extended development time and reduced immature survi ... | 2015 | 25659732 |
| bacillus cereus bacteraemia in a patient of acute myeloid leukaemia. | 2015 | 25657148 | |
| chemical composition and antibacterial activities of seven eucalyptus species essential oils leaves. | in this paper, we have studied the essential oils chemical composition of the leaves of seven eucalyptus species developed in tunisia. eucalyptus leaves were picked from trees growing in different arboretums in tunisia. choucha and mrifeg arboretums located in sedjnene, region of bizerte (choucha: e. maideni, e. astrengens et e. cinerea; mrifeg : e. leucoxylon), korbous arboretums located in the region of nabeul, north east tunisia with sub-humid bioclimate, (e. lehmani), souiniet-ain drahem arb ... | 2015 | 25654423 |
| bioactive prenylated xanthones from the young fruits and flowers of garcinia cowa. | five new xanthones, garciniacowones a-e (1-5), together with 14 known xanthones, 6-19, were isolated from the young fruits and fresh flowers of garcinia cowa. the structures of 1-5 were elucidated by analysis of their 1d and 2d nmr spectra and mass spectrometric data. the compounds 1-19 were tested in vitro for their antimicrobial activity and for their ability to inhibit α-glucosidase. compounds 16 and 17 showed the most potent α-glucosidase inhibitory activity, with ic50 values of 7.8 ± 0.5 an ... | 2015 | 25651042 |
| evaluation of antioxidant, total phenol and flavonoid content and antimicrobial activities of artocarpus altilis (breadfruit) of underutilized tropical fruit extracts. | artocarpus altilis (breadfruit) pulp, peel and whole fruit were extracted with various solvents such as hexane, dichloromethane (dcm) and methanol. the antioxidant activity of these extracts were examined using the stable 2,2-diphenyl-1-picrylhydrazyl (dpph) radical scavenging test. ic50 was 55 ± 5.89 μg/ml for the pulp part of methanol extract. in the β-carotene bleaching assay, the antioxidant activity was 90.02 ± 1.51 % for the positive control (trolox) and 88.34 ± 1.31 % for the pulp part of ... | 2015 | 25649443 |
| microbial diversity of mangrove sediment in shenzhen bay and gene cloning, characterization of an isolated phytase-producing strain of spc09 b. cereus. | phytases hydrolyze phytate to release inorganic phosphate, which decreases the requirement for phosphorus in fertilizers for crops and thus reduces environmental pollutants. this study analyzed microbial communities in rhizosphere sediment, collected in september 2012 from shenzhen bay, guangdong, china, using high-throughput pyrosequencing; the results showed that the dominant taxonomic phyla were chloroflexi, firmicutes, and proteobacteria, and the proportion of the beneficial bacteria, bacill ... | 2015 | 25646962 |
| isolation & identification of bacteria for the treatment of brown crab (cancer pagurus) waste to produce chitinous material. | to isolate bacteria from soil for microbial pretreatment of brown crab (cancer pagurus) shell waste and the production of chitin. | 2015 | 25644656 |
| characterisation of antimicrobial extracts from dandelion root (taraxacum officinale) using lc-spe-nmr. | plant extracts have traditionally been used as sources of natural antimicrobial compounds, although in many cases, the compounds responsible for their antimicrobial efficacy have not been identified. in this study, crude and dialysed extracts from dandelion root (taraxacum officinale) were evaluated for their antimicrobial properties against gram positive and gram negative bacterial strains. the methanol hydrophobic crude extract (dre3) demonstrated the strongest inhibition of microbial growth a ... | 2015 | 25644491 |
| one-day pulsed-field gel electrophoresis protocol for rapid determination of emetic bacillus cereus isolates. | bacillus cereus, the gram-positive and spore-forming ubiquitous bacterium, may cause emesis as the result of food intoxication with cereulide, a heat-stable emetic toxin. rapid determination of cereulide-positive b. cereus isolates is of highest importance due to consequences of this intoxication for human health and life. here we present a 1-day pulsed-field gel electrophoresis for emetic b. cereus isolates, which allows rapid and efficient determination of their genomic relatedness and helps d ... | 2015 | 25639850 |
| protein structure. structure and activity of tryptophan-rich tspo proteins. | translocator proteins (tspos) bind steroids and porphyrins, and they are implicated in many human diseases, for which they serve as biomarkers and therapeutic targets. tspos have tryptophan-rich sequences that are highly conserved from bacteria to mammals. here we report crystal structures for bacillus cereus tspo (bctspo) down to 1.7 å resolution, including a complex with the benzodiazepine-like inhibitor pk11195. we also describe bctspo-mediated protoporphyrin ix (ppix) reactions, including ca ... | 2015 | 25635100 |
| phenolic acids profile, antioxidant and antibacterial activity of chamomile, common yarrow and immortelle (asteraceae). | chamomile, common yarrow and immortelle ethanolic extracts were chemically analysed with respect to phenolics. twelve phenolic acids were separated and identified by hplc-dad and the presence of rosmarinic acid was additionally confirmed by lc-ms. five methods were applied for the evaluation of extracts' antioxidant properties (frap, dpph, abts, chelating activity, briggs-rauscher reaction), while the antibacterial activity was tested against some of the major food-borne pathogens (campylobacter ... | 2014 | 25632475 |
| phylogenetic relationship of phosphate solubilizing bacteria according to 16s rrna genes. | phosphate solubilizing bacteria (psb) can convert insoluble form of phosphorous to an available form. applications of psb as inoculants increase the phosphorus uptake by plant in the field. in this study, isolation and precise identification of psb were carried out in malaysian (serdang) oil palm field (university putra malaysia). identification and phylogenetic analysis of 8 better isolates were carried out by 16s rrna gene sequencing in which as a result five isolates belong to the beta subdiv ... | 2015 | 25632387 |
| quantitative and qualitative evaluation of bio-aerosols in surgery rooms and emergency department of an educational hospital. | bio-aerosols are a potential hazard in hospitals and are mostly produced by hospital staff, patients and visitors. bio-aerosols are solid or liquid particles pending in the air and they consist of aerosols accompanying micro-organisms or organic compounds of micro-organisms such as endotoxin, metabolite, toxin and other parts of organism. those are a potential hazard in hospitals and are mostly produced by hospital staff, patients and visitors. | 2014 | 25632321 |
| an outbreak of gastroenteritis linked to a buffet lunch served at a canberra restaurant. | in 2013, an outbreak of gastrointestinal illness occurred following a buffet lunch at a restaurant in canberra. an investigation was conducted to identify the cause of illness and to implement appropriate public health measures to prevent further disease. we conducted a retrospective cohort study via telephone interviews, using a structured questionnaire developed from the restaurant buffet menu. a case was defined as someone who ate the buffet lunch at the restaurant on the implicated date and ... | 2014 | 25631587 |
| food-grade antimicrobials potentiate the antibacterial activity of 1,2-hexanediol. | preservative agents determining the shelf life of cosmetic products must have effective antimicrobial activity while meeting safety requirements for topical use. in this study, we determined the antimicrobial activity of 1,2-hexanediol against several gram-positive and gram-negative bacteria. antimicrobial susceptibility tests have shown that 1,2-hexanediol exhibits broad-spectrum activity against gram-positive and gram-negative bacteria with mics of 0·5-2% (v/v). the bactericidal concentration ... | 2015 | 25631558 |
| a genomic region involved in the formation of adhesin fibers in bacillus cereus biofilms. | bacillus cereus is a bacterial pathogen that is responsible for many recurrent disease outbreaks due to food contamination. spores and biofilms are considered the most important reservoirs of b. cereus in contaminated fresh vegetables and fruits. biofilms are bacterial communities that are difficult to eradicate from biotic and abiotic surfaces because of their stable and extremely strong extracellular matrix. these extracellular matrixes contain exopolysaccharides, proteins, extracellular dna, ... | 2014 | 25628606 |
| analysis of bacillus cereus toxicity using pcr, elisa and a lateral flow device. | the aim of this study was to evaluate the performance of immunodetection methods and pcr analysis of enterotoxigenic bacillus cereus strains. | 2015 | 25627167 |
| introducing urtica dioica, a native plant of khuzestan, as an antibacterial medicinal plant. | urtica dioica is a flowering plant with long history of use in folk medicine and as a food source. | 2014 | 25625045 |
| evaluation of haccp system implementation on the quality of mixed fresh-cut salad prepared in a university canteen: a case study. | the increasing awareness that foods can represent vehicles for health risk factors has caused scientists and public authorities to multiply their efforts to reduce these risks to within acceptable limits. nevertheless, some challenging issues still remain unsolved and new ones have recently emerged, such as the increase in outbreaks of foodborne diseases originating from the consumption of meals at catering facilities. the study described in this article was aimed at evaluating the microbiologic ... | 2015 | 25619040 |
| effects of climate change on the persistence and dispersal of foodborne bacterial pathogens in the outdoor environment: a review. | according to the intergovernmental panel on climate change (ipcc), warming of the climate system is unequivocal. over the coming century, warming trends such as increased duration and frequency of heat waves and hot extremes are expected in some areas, as well as increased intensity of some storm systems. climate-induced trends will impact the persistence and dispersal of foodborne pathogens in myriad ways, especially for environmentally ubiquitous and/or zoonotic microorganisms. animal hosts of ... | 2016 | 25612827 |
| investigation of antibacterial, acid and bile tolerance properties of lactobacilli isolated from koozeh cheese. | lactobacillus strains are a major part of the probiotics, microflora of the intestine and of fermented dairy products, and are found in a variety of environments. the aim of this study was to find out the ability of bile and acid tolerance and antibacterial properties of the twenty eight isolates of three group lactobacilli namely lactobacillus plantarum, lactobacillus casei and lactobacillus delbruki. for this purpose twenty eight different lactobacillus strains that isolated from koozeh cheese ... | 2012 | 25610566 |
| plasmids are vectors for redundant chromosomal genes in the bacillus cereus group. | prokaryotic plasmids have played significant roles in the evolution of bacterial genomes and have a great impact on the metabolic functions of the host cell. many bacterial strains contain multiple plasmids, but the relationships between bacterial plasmids and chromosomes are unclear. we focused on plasmids from the bacillus cereus group because most strains contain several plasmids. | 2015 | 25608745 |
| squid pen chitin chitooligomers as food colorants absorbers. | one of the most promising applications of chitosanase is the conversion of chitinous biowaste into bioactive chitooligomers (cos). tku033 chitosanase was induced from squid pen powder (spp)-containing bacillus cereus tku033 medium and purified by ammonium sulfate precipitation and column chromatography. the enzyme was relatively more thermostable in the presence of the substrate and had an activity of 93% at 50 °c in a ph 5 buffer solution for 60 min. furthermore, the enzyme used for the cos pre ... | 2015 | 25608726 |
| evaluation of bacillus cereus and bacillus pumilus metabolites for anthelmintic activity. | to assess the anthelmintic acivity of bacillus cereus and bacillus pumilus metabolites. | 2017 | 25598639 |
| characteristics of enterotoxin distribution, hemolysis, lecithinase, and starch hydrolysis of bacillus cereus isolated from infant formulas and ready-to-eat foods. | bacillus cereus is a ubiquitous environmental microbe implicated as a main cause of food poisoning with various symptoms, depending on the strain type and the isolation source. in this study, the potential virulence factors and biochemical properties of b. cereus isolated from infant formulas and ready-to-eat (rte) foods were analyzed and compared. a total of 347 b. cereus strains were isolated and identified from 687 infant food formulas and rte food samples. all the isolates had one or more en ... | 2015 | 25597976 |
| bacteriophage pbc1 and its endolysin as an antimicrobial agent against bacillus cereus. | bacillus cereus is an opportunistic human pathogen responsible for food poisoning and other, nongastrointestinal infections. due to the emergence of multidrug-resistant b. cereus strains, the demand for alternative therapeutic options is increasing. to address these problems, we isolated and characterized a siphoviridae virulent phage, pbc1, and its lytic enzymes. pbc1 showed a very narrow host range, infecting only 1 of 22 b. cereus strains. phylogenetic analysis based on the major capsid prote ... | 2015 | 25595773 |
| finished genome sequence of bacillus cereus strain 03bb87, a clinical isolate with b. anthracis virulence genes. | bacillus cereus strain 03bb87, a blood culture isolate, originated in a 56-year-old male muller operator with a fatal case of pneumonia in 2003. here we present the finished genome sequence of that pathogen, including a 5.46-mb chromosome and two plasmids (209 and 52 kb, respectively). | 2015 | 25593267 |
| antimicrobial potential of cauliflower, broccoli, and okara byproducts against foodborne bacteria. | the antimicrobial potential of cauliflower, broccoli, and okara byproducts was assessed against gram-positive and gram-negative bacteria. salmonella enterica serovar typhimurium, escherichia coli o157:h7, bacillus cereus, and listeria monocytogenes serovar 4b growth behavior was assessed under exposure to 5% vegetable byproducts added to the reference medium, buffered peptone water (0.1% [wt/vol]), at 37°c. although the byproducts were not effective against l. monocytogenes, they were bactericid ... | 2015 | 25587927 |
| electrochemical biosensor for rapid and sensitive detection of magnetically extracted bacterial pathogens. | biological defense and security applications demand rapid, sensitive detection of bacterial pathogens. this work presents a novel qualitative electrochemical detection technique which is applied to two representative bacterial pathogens, bacillus cereus (as a surrogate for b. anthracis) and escherichia coli o157:h7, resulting in detection limits of 40 cfu/ml and 6 cfu/ml, respectively, from pure culture. cyclic voltammetry is combined with immunomagnetic separation in a rapid method requiring ap ... | 2012 | 25585629 |
| slow leakage of ca-dipicolinic acid from individual bacillus spores during initiation of spore germination. | when exposed to nutrient or nonnutrient germinants, individual bacillus spores can return to life through germination followed by outgrowth. laser tweezers, raman spectroscopy, and either differential interference contrast or phase-contrast microscopy were used to analyze the slow dipicolinic acid (dpa) leakage (normally ∼20% of spore dpa) from individual spores that takes place prior to the lag time, tlag, when spores begin rapid release of remaining dpa. major conclusions from this work with b ... | 2015 | 25583976 |
| propidium monoazide combined with real-time pcr for selective detection of viable staphylococcus aureus in milk powder and meat products. | staphylococcus aureus is a spherical, gram-positive, pathogenic bacterium commonly associated with bovine mastitis and clinical infections. it is also recognized as a pathogen that causes outbreaks of food poisoning. the objective of this study was to develop and evaluate a rapid and reliable technique that combines propidium monoazide (pma) staining with real-time quantitative (q)pcr to detect and quantify viable cells of staph. aureus in milk powder and meat products. the inclusivity and exclu ... | 2015 | 25582587 |
| morphological and mechanical imaging of bacillus cereus spore formation at the nanoscale. | bacteria from the genus bacillus are able to transform into metabolically dormant states called (endo) spores in response to nutrient deprivation and other harsh conditions. these morphologically distinct spores are fascinating constructs, amongst the most durable cells in nature, and have attracted attention owing to their relevance in food-related illnesses and bioterrorism. observing the course of bacterial spore formation (sporulation) spatially, temporally and mechanically, from the vegetat ... | 2015 | 25581787 |
| impacts of sporulation temperature, exposure to compost matrix and temperature on survival of bacillus cereus spores during livestock mortality composting. | to investigate impact of sporulation and compost temperatures on feasibility of composting for disposal of carcasses contaminated with bacillus anthracis. | 2015 | 25580774 |
| characterization of a nanoscale s-layer protein based template for biomolecular patterning. | well organized template for biomolecular conjugation is the foundation for biosensing. most of the current devices are fabricated using lithographic patterning processes and self-assembly monolayer (sam) methods. however, the research toward developing a sub-10 nm patterned, self-regenerated template on various types of substrates is limited, mainly due to the limited functional groups of the building material. bacterial surface layer proteins (s-layer proteins) can self-assemble into ordered la ... | 2014 | 25570568 |
| hemorrhagic bullous lesions due to bacillus cereus in a cirrhotic patient. | 2014 | 25566578 | |
| antimicrobial activities of three medicinal plants and investigation of flavonoids of tripleurospermum disciforme. | rosa damascena, tripleurospermum disciforme and securigera securidaca were used as disinfectant agents and for treatment of some disease in folk medicine of iran. the antimicrobial effects of different fractions of seeds extract of s. securidaca, petals extract of r. damascena and aerial parts extract of t. disciforme were examined against some gram positive, gram negative and fungi by cup plate diffusion method. the petroleum ether and chloroform fractions of s. securidaca showed antibacterial ... | 2015 | 25561928 |
| improved efficiency and stability of secnidazole - an ideal delivery system. | secnidazole (α,2-dimethyl-5-nitro-1h-imidazole-1-ethanol) is a highly effective drug against a variety of g(+)/g(-) bacteria but with significant side effects because it is being used in very high concentration. in this study, gold nanoparticles (gnps) were selected as a vehicle to deliver secnidazole drug at the specific site with more accuracy which made the drug highly effective at substantially low concentrations. the as-synthesized gnps were capped with human serum albumin (hsa) and subsequ ... | 2015 | 25561882 |
| carvacrol suppresses high pressure high temperature inactivation of bacillus cereus spores. | the inactivation of bacterial spores generally proceeds faster and at lower temperatures when heat treatments are conducted under high pressure, and high pressure high temperature (hpht) processing is, therefore, receiving an increased interest from food processors. however, the mechanisms of spore inactivation by hpht treatment are poorly understood, particularly at moderately elevated temperature. in the current work, we studied inactivation of the spores of bacillus cereus f4430/73 by hpht tr ... | 2015 | 25560915 |
| stem bark extract and fraction of persea americana (mill.) exhibits bactericidal activities against strains of bacillus cereus associated with food poisoning. | the study investigates the in vitro antibacterial potentials of stem bark extracts of persea americana on strains of bacillus cereus implicated in food poisoning. the crude stem bark extracts and butanolic fraction at a concentration of 25 mg/ml and 10 mg/ml, respectively, exhibited antibacterial activities against test isolates. the zones of inhibition exhibited by the crude extract and the fraction ranged between 10 mm and 26 mm, while the minimum inhibitory concentration values ranged between ... | 2014 | 25558854 |
| toxigenic potential and heat survival of spore-forming bacteria isolated from bread and ingredients. | fifty-four spore-forming bacterial strains isolated from bread ingredients and bread, mainly belonging to the genus bacillus (including bacillus cereus), together with 11 reference strains were investigated to evaluate their cytotoxic potential and heat survival in order to ascertain if they could represent a risk for consumer health. therefore, we performed a screening test of cytotoxic activity on ht-29 cells using bacterial culture filtrates after growing bacterial cells in brain heart infusi ... | 2015 | 25555227 |
| antimicrobial and inhibition on heat-induced protein denaturation of constituents isolated from polygonatum verticillatum rhizomes. | this study was designed to assess the susceptibility of various microorganisms and inhibition on heat-induced protein denaturation against diosgenin and santonin, isolated from polygonatum verticillatum rhizomes. both diosgenin and santonin showed significant zone of inhibition when studied against various gram-positive (bacillus subtilis, bacillus cereus, staphylococcus aureus and staphylococcus epidermidis) and gram-negative bacteria (escherichia coli and salmonella typhi). in antifungal assay ... | 2015 | 25553804 |
| the bacillus cereus group is an excellent reservoir of novel lanthipeptides. | lantibiotics are ribosomally synthesized peptides that contain multiple posttranslational modifications. research on lantibiotics has increased recently, mainly due to their broad-spectrum antimicrobial activity, especially against some clinical gram-positive pathogens. many reports about various bacteriocins in the bacillus cereus group have been published, but few were about lantibiotics. in this study, we identified 101 putative lanthipeptide gene clusters from 77 out of 223 strains of this g ... | 2015 | 25548056 |
| correction: genomic comparison of 93 bacillus phages reveals 12 clusters, 14 singletons and remarkable diversity. | the bacillus genus of firmicutes bacteria is ubiquitous in nature and includes one of the best characterized model organisms, b. subtilis, as well as medically significant human pathogens, the most notorious being b. anthracis and b. cereus. as the most abundant living entities on the planet, bacteriophages are known to heavily influence the ecology and evolution of their hosts, including providing virulence factors. thus, the identification and analysis of bacillus phages is critical to underst ... | 2014 | 25547158 |
| spirotetronate antibiotics with anti-clostridium activity from actinomadura sp. 2eps. | the rare actinomycetes strain 2eps was isolated from soil and analysis of cultural, morphological characteristics, diaminopimelic acid content of its cell wall, and 16s rrna gene sequence indicates that 2eps belongs to genus actinomadura. in addition, neighbor-joining phylogenetic tree also confirmed the relationships of this strain to other members of actinomadura. a butanol extract with antibacterial activity was purified by reversed-phase chromatography to obtain three bioactive compounds, de ... | 2015 | 25543910 |
| biosynthesis of hematite nanoparticles and its cytotoxic effect on hepg2 cancer cells. | iron oxide nanoparticles were gaining significant importance in a variety of applications due to its paramagnetic properties and biocompatibility. various chemical methods were employed for hematite nanoparticle synthesis which require special equipment or a complex production process. in this study, protein capped crystalline hexagonal hematite (α-fe2o3) nanoparticles were synthesized by green approach using culture supernatant of a newly isolated bacterium, bacillus cereus svk1 at ambient cond ... | 2015 | 25542166 |
| activity against microorganisms affecting cellulosic objects of the volatile constituents of leonotis nepetaefolia from nicaragua. | the chemical composition of the essential oil from aerial parts of l. nepetaefolia (l.) r. br. collected in nicaragua was evaluated by gc and gc-ms. the main components were (z)-phytol (22.8%), caryophyllene oxide (18.9%) and hexahydrofarnesylacetone (9.0%). the antibacterial activity against several gram + and gram - bacteria, including bacillus cereus and b. subtilis, both infesting historical cellulosic material, was also determined. b. cereus, b. subtilis and staphylococcus epidermis were th ... | 2014 | 25532300 |
| chemical composition and biological activity of pulicaria vulgaris essential oil from iran. | the present study investigated the chemical composition of the essential oil (eo) from aerial parts (flowering stage) of pulicaria vulgaris gaertn. by gc-ms. also, the antimicrobial activity of the eo against gram-positive bacteria (bacillus cereus and staphylococcus aureus), gram-negative bacteria (escherichia coli and pseudomonas aeruginosa) and fungi (aspergillus niger and candida albicans) was tested. in total, 23 compounds were recognized, accounting for 98.08% of the eo. the main compounds ... | 2014 | 25532299 |
| characterization of a novel thermostable n-acylhomoserine lactonase from the thermophilic bacterium thermaerobacter marianensis. | thermaerobacter marianensis is an extremely thermophilic bacterium, which was isolated from the mariana trench, with an optimal growth temperature of approximately 75 °c. n-acylhomoserine lactone (ahl) is a quorum-sensing signal molecule used by many gram-negative bacteria. here, we report the identification of an ahl-degrading gene homolog (designated aiit) in the genome of t. marianensis jcm 10246. aiit has 59.7%, 21.2%, and 11.2% identity to ahls from solibacillus silvestris, aiia from bacill ... | 2015 | 25529553 |
| characteristics and phylogeny of bacillus cereus strains isolated from maari, a traditional west african food condiment. | maari is a spontaneously fermented food condiment made from baobab tree seeds in west african countries. this type of product is considered to be safe, being consumed by millions of people on a daily basis. however, due to the spontaneous nature of the fermentation the human pathogen bacillus cereus occasionally occurs in maari. this study characterizes succession patterns and pathogenic potential of b. cereus isolated from the raw materials (ash, water from a drilled well (dw) and potash), seed ... | 2015 | 25528535 |
| draft genome sequence of new bacillus cereus strain tsu1. | this paper reports the draft genome sequence of new bacillus cereus strain tsu1, isolated on an agar-cellulose plate. the draft genome sequence is 5.81 mb, revealing 5,673 coding sequences. it contains genes for cellulose-degradation and biosynthesis pathways of polyhydroxybutyrate (phb) and 8 rrna genes (5s, 16s, and 23s). | 2014 | 25523768 |
| evaluating the efficiency of humic acid to remove micro-organisms from denture base material. | to evaluate the efficiency of humic acid substances on removing micro-organisms from denture base materials. | 2016 | 25523597 |
| quantitative analysis of cereulide toxin from bacillus cereus in rice and pasta using synthetic cereulide standard and 13c6-cereulide standard - a short validation study. | a single laboratory validation study of a rapid and sensitive quantitative method for the analysis of cereulide toxin produced by bacillus cereus using ultra high performance liquid chromatography-electrospray-tandem mass spectrometry is presented. the analysis of this cyclic peptide toxin was validated for pasta and rice samples using a newly presented synthetic cereulide peptide standard, together with 13c6-cereulide that previously have not been commercially available. the use of cereulide st ... | 2014 | 25514093 |
| [electron microscopy of the surfaces of bacillary spores]. | the surface structures of the spores of bacillus. cereus, b. thuringiensis, and brevibacillus laterosporus were studied by transmission and scanning electron microscopy. platinum deposition and negative staining with uranyl acetate revealed appendages and exosporium in b. thuringiensis and b. cereus. the exosporium structure was visualized by negative staining and ultrathin sectioning. for staining the exosporium polysaccharide, alcian blue was used during fixation. the results obtained show the ... | 2014 | 25509408 |
| [purification of recombinant bacillus cereus resd-rese proteins expressed in escherichia coli strains]. | recombinant e. coli strains expressing the bacillus cereus atcc 14579t resd and resegenes fused with the ubiquitin gene were constructed, and purification of the resd and rese proteins was performed. the approach used in the study allowed us to increase the protein yield of the electrophoretic homogeneous resd andrese proteins without denaturation steps up to 150 mg per gram of wet cell weight. | 2014 | 25507776 |
| single cell profiling of surface carbohydrates on bacillus cereus. | cell surface carbohydrates are important to various bacterial activities and functions. it is well known that different types of bacillus display heterogeneity of surface carbohydrate compositions, but detection of their presence, quantitation and estimation of variation at the single cell level have not been previously solved. here, using atomic force microscopy (afm)-based recognition force mapping coupled with lectin probes, the specific carbohydrate distributions of n-acetylglucosamine and m ... | 2015 | 25505137 |
| a common active site of polyhydroxyalkanoate synthase from bacillus cereus yb-4 is involved in polymerization and alcoholysis reactions. | polyhydroxyalkanoate (pha) synthase from bacillus cereus yb-4 (pharcyb4) catalyzes not only pha polymerization but also alcoholytic cleavage of pha chains. the alcoholysis activity of pharcyb4 is expressed when a hydroxyacyl-coa monomer is absent but an alcohol compound is present. in this study, we performed alanine mutagenesis of the putative catalytic triad (cys(151), asp(306), and his(335)) in the phacyb4 subunit to identify the active site residues for polymerization and alcoholysis activit ... | 2015 | 25503319 |
| the cyclic antibacterial peptide enterocin as-48: isolation, mode of action, and possible food applications. | enterocin as-48 is a circular bacteriocin produced by enterococcus. it contains a 70 amino acid-residue chain circularized by a head-to-tail peptide bond. the conformation of enterocin as-48 is arranged into five alpha-helices with a compact globular structure. enterocin as-48 has a wide inhibitory spectrum on gram-positive bacteria. sensitivity of gram-negative bacteria increases in combination with outer-membrane permeabilizing treatments. eukaryotic cells are bacteriocin-resistant. this catio ... | 2014 | 25493478 |
| microbiome of the placenta in pre-eclampsia supports the role of bacteria in the multifactorial cause of pre-eclampsia. | this study was aimed at detecting, identifying, quantifying and comparing the bacteria present in the placental tissues of women with pre-eclampsia with that of normotensive pregnant women. | 2015 | 25492799 |
| the denitrification characteristics of pseudomonas stutzeri sc221-m and its application to water quality control in grass carp aquaculture. | to reduce ammonium and nitrite in aquaculture water, an isolate of the denitrifying bacterium pseudomonas stutzeri, sc221-m, was obtained. the effects of various nitrogen and carbon sources, the ratio of carbon to nitrogen and temperature on bacterial growth, denitrification rates and the expression levels of nirs and nosz in sc221-m were studied. the following conditions were determined to be optimal for growth and denitrification in sc221-m: nano2 as the nitrogen source, sodium citrate as the ... | 2014 | 25489740 |
| bacillus cereus in personal care products: risk to consumers. | bacillus cereus is ubiquitous in nature and thus occurs naturally in a wide range of raw materials and foodstuffs. b. cereus spores are resistant to desiccation and heat and able to survive dry storage and cooking. vegetative cells produce several toxins which on ingestion in sufficient numbers can cause vomiting and/or diarrhoea depending on the toxins produced. gastrointestinal disease is commonly associated with reheated or inadequately cooked foods. in addition to being a rare cause of sever ... | 2015 | 25482451 |
| a halotolerant thermostable lipase from the marine bacterium oceanobacillus sp. pumb02 with an ability to disrupt bacterial biofilms. | a halotolerant thermostable lipase was purified and characterized from the marine bacterium oceanobacillus sp. pumb02. this lipase displayed a high degree of stability over a wide range of conditions including ph, salinity, and temperature. it was optimally active at 30 °c and ph 8.0 respectively and was stable at higher temperatures (50-70 °c) and alkaline ph. the molecular mass of the lipase was approximately 31 kda based on sds-page and maldi-tof fingerprint analysis. conditions for enhanced ... | 2014 | 25482232 |
| inactivation of chemical and heat-resistant spores of bacillus and geobacillus by nitrogen cold atmospheric plasma evokes distinct changes in morphology and integrity of spores. | bacterial spores are resistant to severe conditions and form a challenge to eradicate from food or food packaging material. cold atmospheric plasma (cap) treatment is receiving more attention as potential sterilization method at relatively mild conditions but the exact mechanism of inactivation is still not fully understood. in this study, the biocidal effect by nitrogen cap was determined for chemical (hypochlorite and hydrogen peroxide), physical (uv) and heat-resistant spores. the three diffe ... | 2015 | 25481059 |
| reducing activity, glucose metabolism and acid tolerance response of bacillus cereus grown at various ph and oxydo-reduction potential levels. | bacillus cereus is a major foodborne bacterial pathogen able to survive a large number of physical-chemical stresses. b. cereus encounters different ph and redox potential (eh7) levels during its passage through the gastrointestinal tract. analysis of the combined influence of ph and redox stresses on b. cereus f4430/73 physiology found that b. cereus f4430/73 growth at ph 7.0 at 37 °c had strong reducing capacities, with a total change of 315 mv from an initial redox value of +214 ± 17 mv. the ... | 2015 | 25475301 |
| genetic discrimination of foodborne pathogenic and spoilage bacillus spp. based on three housekeeping genes. | bacillus genus includes foodborne pathogenic and spoilage-associated species, such as bacillus cereus, bacillus licheniformis, bacillus subtilis and bacillus pumilus. bacillus is also a heterogeneous genus that includes closely related species that are difficult to discriminate among, especially when well-conserved genes such as 16s rrna and 23s rrna are considered. the main goal of the present work was to study the usefulness of three housekeeping genes, the tu elongation factor (tuf), the dna ... | 2015 | 25475298 |
| identification of single nucleotide polymorphisms (snps) in the 16s rrna gene of foodborne bacillus spp. | the main goal of this work was the identification of single nucleotide polymorphisms (snps) in the 16s rrna gene of foodborne bacillus spp. that may be useful for typing purposes. these species include, among others, bacillus cereus, an important pathogenic species involved in food poisoning, and bacillus licheniformis, bacillus subtilis and bacillus pumilus, which are causative agents of food spoilage described as responsible for foodborne disease outbreaks. with this purpose in mind, 52 bacill ... | 2015 | 25475292 |
| a case of intoxication due to a highly cytotoxic bacillus cereus strain isolated from cooked chicken. | outbreaks of bacillus cereus infection/intoxication are not commonly reported because symptoms are often mild, and the disease is self-limiting. however, hypervirulent strains increase health risks. we report a case, which occurred in argentina, of severe food poisoning illness on a healthy adult woman associated to b. cereus strain mvl2011. the studied strain was highly cytotoxic, showed high ability to detach caco-2 cells and was positive for the hbla, hblb, and hblc genes of the hbl complex, ... | 2015 | 25475284 |
| novel polycarboxylate porphyrins: synthesis, characterization, photophysical properties and preliminary antimicrobial study against gram-positive bacteria. | we describe the synthesis, characterization and photophysical properties of two new polycarboxylic photosensitizers. owing to their structural design, these two compounds show water solubilities larger than natural carboxylic photosensitizers (e.g., protoporphyrin ix, hematoporphyrin, etc.) and also good singlet oxygen quantum yields. these compounds were tested as photo-antimicrobial agents against staphylococcus aureus and bacillus cereus strains. results reveal that their photocytotoxicities ... | 2015 | 25475206 |
| detection of toxigenic bacillus cereus strains isolated from vegetables in mexico city. | bacillus cereus can cause diarrhea and emetic syndromes after ingestion of food contaminated with it. this ability is due to the production of enterotoxins by this microorganism, these being the hemolysin bl complex, which is involved in the diarrheal syndrome, and cereulide, which is responsible for the emetic syndrome. the detection of genes associated with the production of these toxins can predict the virulence of strains isolated from contaminated food. in this paper, we analyzed 100 sample ... | 2014 | 25474064 |
| surfactins in natto: the surfactin production capacity of the starter strains and the actual surfactin contents in the products. | surfactin-type lipopeptides are suspected of being implicated in the rare food poisonings caused by bacillus species outside the bacillus cereus cluster. in order to get information on surfactin levels in actual human foods, bacilli from three commercial samples of a japanese traditional bean product, natto, were isolated in order to clarify their potential to produce the suspect lipopeptides. the isolated bacilli were characterized as bacillus subtilis. they were β-hemolytic and gave a positive ... | 2014 | 25474063 |
| microbiological quality of seasoned roasted laver and potential hazard control in a real processing line. | microbiological quality of laver, one of the edible seaweeds, has not been reported in a real processing line. laver or supplements were collected from six manufacturers (a to f) to assess potential microbiological hazards and the critical control points in commercial processing lines. aerobic plate counts (apc), coliform counts, bacillus cereus, staphylococcus aureus, and vibrio parahaemolyticus were enumerated, and the presence of b. cereus, listeria monocytogenes, salmonella, s. aureus, and v ... | 2014 | 25474052 |
| effect of white mustard essential oil on the growth of foodborne pathogens and spoilage microorganisms and the effect of food components on its efficacy. | antimicrobial preservative compounds are added to foods to target specific pathogens and spoilage organisms. white mustard essential oil (wmeo) is an extract that contains 4-hydroxybenzyl isothiocyanate, a compound which has been demonstrated to have antimicrobial activity in limited studies. the objective of this research was to determine the in vitro antimicrobial activity of wmeo against gram-positive and gram-negative spoilage and pathogenic bacteria and determine the effect of food componen ... | 2014 | 25474051 |
| modeling of bacillus cereus growth in brown rice submitted to a combination of ultrasonication and slightly acidic electrolyzed water treatment. | the combined effects of ultrasonication and slight acidic electrolyzed water were investigated to improve the microbial safety of brown rice against bacillus cereus infection and to evaluate the growth kinetics of these bacteria during storage of untreated and treated rice at various temperatures (5, 10, 15, 20, 25, 30, and 35°c). the results indicate that this combination treatment was bactericidal against b. cereus, resulting in an approximately 3.29-log reduction. although b. cereus can be ef ... | 2014 | 25474049 |
| antibiotic-resistant heterotrophic plate count bacteria and amoeba-resistant bacteria in aquifers of the mooi river, north west province, south africa. | groundwater in the mooi river catchment is prone to mining, agricultural, municipal and septic tank pollution. in this study physico-chemical and microbiological parameters were determined using appropriate methods. bacterial isolates were identified by 16s rrna sequencing (heterotrophic plate count (hpc) bacteria and amoeba-resistant bacteria (arb)) and multiplex polymerase chain reaction (escherichia coli). antibiotic resistance tests were also performed. physico-chemical parameters were gener ... | 2014 | 25473993 |
| prevalence, genetic diversity, and antibiotic resistance of bacillus cereus isolated from korean fermented soybean products. | bacillus cereus contamination is a major food safety problem for korean fermented soybean products, but few studies have assessed its potential to cause foodborne illness. the objectives of this study were to investigate the prevalence and characteristics of b. cereus isolated from korean fermented soybean products. b. cereus was detected in 110 of 162 (67.9%) samples. the highest b. cereus frequency was observed in deonjang (68 of 93 samples, 73.1%) and cheonggukjang (18 of 25, 72.0%); however, ... | 2015 | 25472031 |
| the analysis of distribution of multidrug resistant pseudomonas and bacillus species from burn patients and burn ward environment. | infections caused by multidrug resistant bacteria act as a risk factor for mortality in burns patients. so keeping in view the crucial importance of reliable therapeutic decisions of multidrug resistance bacteria and role of hospital environment in bacteria colonization, our study is based on the evaluation of distribution of pseudomonas sp. and bacillus sp. in burn patients and burn ward environment. | 2015 | 25468476 |
| effect of packaging technology on microbiological and sensory quality of a cooked blood sausage, morcela de arroz, from monchique region of portugal. | morcela de arroz (ma), a popular portuguese blood sausage, with high ph and water activity (aw), is traditionally commercialized without preservatives and unpacked. this study evaluated the best packaging solution to extend ma shelf life stored at 4±1°c for 44days: without packaging (wp), vacuum (vp) and modified atmosphere packaging (map) (80% co2; 20% n2). mesophilic (mtvc), psychrotrophic (ptvc), lactic acid bacteria (lab), pseudomonads, molds and yeasts, enterobacteriaceae, listeria monocyto ... | 2015 | 25462380 |
| integrative approach to produce hydrogen and polyhydroxybutyrate from biowaste using defined bacterial cultures. | biological production of hydrogen (h2) and polyhydroxybutyrate (phb) from pea-shell slurry (pss) was investigated using defined mixed culture (mmc4, composed of enterobacter, proteus, bacillus spp.). under batch culture, 19.0lh2/kg of pss (total solid, ts, 2%w/v) was evolved. using effluent from the h2 producing stage, bacillus cereus egu43 could produce 12.4% (w/w) phb. dilutions of pss hydrolysate containing glucose (0.5%, w/v) resulted in 45-75lh2/kg ts fed and 19.1% (w/w) of phb content. und ... | 2015 | 25460994 |
| aerobic biodegradation of odorous dimethyl disulfide in aqueous medium by isolated bacillus cereus gigan2 and identification of transformation intermediates. | a novel, flagellated, rod-shape, gram-positive facultative aerobe, was isolated and identified as bacillus cereus gigan2. it can effectively remove model odorous organics dimethyl disulfide (dmds) in aqueous solution under aerobic conditions. initial concentration, ph value and temperature played important role in dmds biodegradation, and up to 100% of 10mgl(-1) of dmds could be removed within 96h under the optimum conditions (30°c, ph 7.0 and 200rpm) with a maximum biodegradation rate constant ... | 2015 | 25459868 |
| antibacterial and cytotoxic potential of silver nanoparticles synthesized using latex of calotropis gigantea l. | the present study aimed to synthesis silver nanoparticles (agnps) in a greener route using aqueous latex extract of calotropis gigantea l. toward biomedical applications. initially, synthesis of agnps was confirmed through uv-vis spectroscopy which shows the surface plasmonic resonance peak (spr) at 420 nm. fourier transform infrared spectroscopy (ftir) analysis provides clear evidence that protein fractions present in the latex extract act as reducing and stabilizing bio agents. energy dispersi ... | 2015 | 25459618 |
| an emetic bacillus cereus outbreak in a kindergarten: detection and quantification of critical levels of cereulide toxin. | a bacillus cereus-related emetic outbreak was reported in a belgian kindergarten. high levels of emetic b. cereus (>1.5e+07 colony-forming units/g) were detected in the food leftovers, and the presence of an emetic strain was confirmed in feces. emetic toxin levels ranging up to 4.2 μg/g were also quantified in the leftovers by liquid chromatography coupled to tandem mass spectrometry (lc-ms(2)) analysis. those levels, although moderate in comparison with earlier published intoxications, provoke ... | 2015 | 25457101 |
| brex is a novel phage resistance system widespread in microbial genomes. | the perpetual arms race between bacteria and phage has resulted in the evolution of efficient resistance systems that protect bacteria from phage infection. such systems, which include the crispr-cas and restriction-modification systems, have proven to be invaluable in the biotechnology and dairy industries. here, we report on a six-gene cassette in bacillus cereus which, when integrated into the bacillus subtilis genome, confers resistance to a broad range of phages, including both virulent and ... | 2015 | 25452498 |
| association of a zn(2+) containing metallo β-lactamase with the anticancer antibiotic mithramycin. | pathogenic bacteria that are resistant to β-lactam antibiotics mostly utilize serine β-lactamases to degrade the antibiotics. current studies have shown that different subclasses of metallo β-lactamases (e[mbl]) are involved in the defense mechanism of drug resistant bacteria. here we report that the zn(2+) containing subclass b1 e[mbl] from bacillus cereus binds to a naturally occurring anti-cancer drug mithramycin (mtr). spectroscopic (cd and fluorescence) and isothermal titration calorimetry ... | 2015 | 25450021 |
| establishing a synthetic pathway for high-level production of 3-hydroxypropionic acid in saccharomyces cerevisiae via β-alanine. | microbial fermentation of renewable feedstocks into plastic monomers can decrease our fossil dependence and reduce global co2 emissions. 3-hydroxypropionic acid (3hp) is a potential chemical building block for sustainable production of superabsorbent polymers and acrylic plastics. with the objective of developing saccharomyces cerevisiae as an efficient cell factory for high-level production of 3hp, we identified the β-alanine biosynthetic route as the most economically attractive according to t ... | 2015 | 25447643 |
| phospholipase c from pseudomonas aeruginosa and bacillus cereus; characterization of catalytic activity. | to study characteristics of phospholipases c (plcs), their importance for producing microorganisms as well as the potential of their use for industrial purposes. | 2014 | 25441984 |
| antibacterial activity and genotypic-phenotypic characteristics of bacteriocin-producing bacillus subtilis kku213: potential as a probiotic strain. | the antimicrobial activity and probiotic properties of bacillus subtilis strain kku213, isolated from local soil, were investigated. the cell-free supernatant (cfs) of a kku213 culture containing crude bacteriocins exhibited inhibitory effects on gram-positive bacteria, including bacillus cereus, listeria monocytogenes, micrococcus luteus, and staphylococcus aureus. the antibacterial activity of the cfs precipitated with 40% ammonium sulfate (as) remained even after treatment at 60 and 100 °c, a ... | 2015 | 25440998 |
| probabilistic exposure assessment model to estimate aseptic-uht product failure rate. | aseptic-ultra-high-temperature (uht) products are manufactured to be free of microorganisms capable of growing in the food at normal non-refrigerated conditions at which the food is likely to be held during manufacture, distribution and storage. two important phases within the process are widely recognised as critical in controlling microbial contamination: the sterilisation steps and the following aseptic steps. of the microbial hazards, the pathogen spore formers clostridium botulinum and baci ... | 2015 | 25440556 |
| antimicrobial and anti-inflammatory activities of pleurostylia capensis turcz (loes) (celastraceae). | pleurostylia capensis is a large tree that can reach the maximum height of 20 m long, and it have been traditionally used as cosmetic, for steam bath, ritual body wash, and as a purgative to treat symptoms of witchcraft. using ethanol, chloroform, dichloromethane (dcm), ethyl acetate (ea), and water extracts, leaves, bark and roots of pleurostylia capensis were investigated scientifically for their effectiveness in antimicrobial, antioxidant and anti-inflammatory activities using standard method ... | 2014 | 25435632 |
| [purification of recombinant bacillus cereus resd-rese proteins expressed in escherichia coli strains]. | recombinant e. coli strains expressing the bacillus cereus atcc 14579t resd and resegenes fused with the ubiquitin gene were constructed, and purification of the resd and rese proteins was performed. the approach used in the study allowed us to increase the protein yield of the electrophoretic homogeneous resd andrese proteins without denaturation steps up to 150 mg per gram of wet cell weight. | 2014 | 25434178 |
| pharmacological basis for the medicinal use of linum usitatissimum (flaxseed) in infectious and non-infectious diarrhea. | linum usitatissimum, commonly known as flaxseed has traditionally been used for the management of diarrhea and gastrointestinal infections. this study was planned to assess pharmacological basis for the medicinal use of flaxseed in infectious and non-infectious diarrhea. | 2015 | 25433250 |
| production and purification of anti-bacterial biometabolite from wild-type lactobacillus, isolated from fermented bamboo shoot: future suggestions and a proposed system for secondary metabolite onsite recovery during continuous fermentation. | wild-type lactobacillus isolated form khorisa, a fermented bamboo shoot product of assam, india were evaluated for production anti-bacterial secondary biometabolites, against staphylococcus aureus. submerged fermentation technique was used for the production of secondary anti-microbial biometabolite by a single wild-type lactobacillus strain, which tested positive for the release of anti-bacterial factor(s). crude cell-free supernatant was obtained, followed by extraction in water-immiscible sol ... | 2015 | 25432345 |
| cytochrome c551 and the cytochrome c maturation pathway affect virulence gene expression in bacillus cereus atcc 14579. | loss of the cytochrome c maturation system in bacillus cereus results in increased transcription of the major enterotoxin genes nhe, hbl, and cytk and the virulence regulator plcr. increased virulence factor production occurs at 37°c under aerobic conditions, similar to previous findings in bacillus anthracis. unlike b. anthracis, much of the increased virulence gene expression can be attributed to loss of only c551, one of the two small c-type cytochromes. additional virulence factor expression ... | 2015 | 25422307 |
| in vitro antioxidant, antimicrobial and anti-proliferative activities of purple potato extracts (solanum tuberosum cv vitelotte noire) following simulated gastro-intestinal digestion. | analyses of antioxidant and in vitro antimicrobial and anti-proliferative activities of anthocyanin-rich extracts from purple potatoes, solanum tuberosum l. cv vitelotte noire (solanaceae), were performed by simulating both a domestic cooking process and human digestion. extracts of crude and cooked purple potato did not exhibit antimicrobial activity against the tester strains: bacillus cereus, escherichia coli and pseudomonas aeruginosa. the behaviour changed after the simulated gastrointestin ... | 2015 | 25420792 |
| screening of microorganisms from deep-sea mud for antarctic krill (euphausia superba) fermentation and evaluation of the bioactive compounds. | twelve kinds of strains were isolated from deep-sea mud which can use antarctic krill powder as the sole carbon/nitrogen source. these strains were identified by 16s rdna sequence analysis and grouped into eight different genera, including bacillus, shewanella, psychrobacter, klebsiella, macrococcus, aeromonas, acinetobacter, and saccharomyces. after fermentation of antarctic krill powder using these strains, bioactive compounds including total phenolics, free amino acids, and enzyme activities ... | 2015 | 25416479 |
| kawasaki disease-specific molecules in the sera are linked to microbe-associated molecular patterns in the biofilms. | kawasaki disease (kd) is a systemic vasculitis of unknown etiology. the innate immune system is involved in its pathophysiology at the acute phase. we have recently established a novel murine model of kd coronary arteritis by oral administration of a synthetic microbe-associated molecular pattern (mamp). on the hypothesis that specific mamps exist in kd sera, we have searched them to identify kd-specific molecules and to assess the pathogenesis. | 2014 | 25411968 |
| comparison of 3 selective media for enumeration of bacillus cereus in several food matrixes. | in this study, we compared the inclusivity, exclusivity, recoverability, and selectivity of the 3 selective agars (mannitol yolk polymyxin b agar [mypa], polymyxin pyruvate egg yolk mannitol bromothymol blue agar [pemba], and brillance bacillus cereus agar [bbc agar]) for bacillus cereus (b. cereus) from pure culture and several food samples. bbc agar showed greater exclusivity and selectivity in pure culture and in foods with high background flora, respectively; however, all the tested media sh ... | 2014 | 25399752 |
| infrared decontamination of oregano: effects on bacillus cereus spores, water activity, color, and volatile compounds. | infrared (ir) heating, a novel technology for decontaminating oregano, was evaluated by investigating the reduction of inoculated bacillus cereus spores and the effect on water activity (a(w)), color, and headspace volatile compounds after exposure to ir treatment. conditioned oregano (a(w) 0.88) was ir-treated in a closed heating unit at 90 and 100 °c for holding times of 2 and 10 min, respectively. the most successful reduction in b. cereus spore numbers (5.6 log units) was achieved after a ho ... | 2014 | 25393824 |