Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| rapid evolution of microbe-mediated protection against pathogens in a worm host. | microbes can defend their host against virulent infections, but direct evidence for the adaptive origin of microbe-mediated protection is lacking. using experimental evolution of a novel, tripartite interaction, we demonstrate that mildly pathogenic bacteria (enterococcus faecalis) living in worms (caenorhabditis elegans) rapidly evolved to defend their animal hosts against infection by a more virulent pathogen (staphylococcus aureus), crossing the parasitism-mutualism continuum. host protection ... | 2016 | 26978164 |
| pilot screening to determine antimicrobial synergies in a multidrug-resistant bacterial strain library. | with the rise in multidrug-resistant (mdr) bacterial infections, there has been increasing interest in combinations of ≥2 antimicrobial agents with synergistic effects. we established an mdr bacterial strain library to screen for in vitro antimicrobial synergy by using a broth microdilution checkerboard method and high-throughput luciferase-based bacterial cell viability assay. in total, 39 mdr bacterial strains, including 23 carbapenem-resistant gram-negative bacteria, 9 vancomycin-intermediate ... | 2016 | 26974861 |
| chemical composition, cytotoxic and antibacterial activities of the essential oil from the tunisian ononis angustissima l. (fabaceae). | the chemical composition, cytotoxic and antibacterial activities of the hydrodistilled essential oil of the aerial parts of ononis angustissima from south tunisia has been evaluated. the oil yield was 0.04% (w/w). the chemical composition, determined by gc and gc-ms is reported for the first time. forty-five components, accounting for 93.7% of the total oil have been identified. the oil was characterized by a high proportion of oxygenated sesquiterpenes (33.2%), followed by sesquiterpene hydroca ... | 2016 | 26972463 |
| l-lactic acid production by combined utilization of agricultural bioresources as renewable and economical substrates through batch and repeated-batch fermentation of enterococcus faecalis rky1. | enterococcus faecalis rky1 was used to produce l-lactic acid from hydrol, soybean curd residues (scr), and malt. hydrol was efficiently metabolized to l-lactic acid with optical purity of >97.5%, though hydrol contained mixed sugars such as glucose, maltose, maltotriose, and maltodextrin. combined utilization of hydrol, scr, and malt was enough to sustain lactic acid fermentation by e. faecalis rky1. in order to reduce the amount of nitrogen sources and product inhibition, cell-recycle repeated- ... | 2016 | 26970921 |
| enterococcus faecalis ebp pili are important for cell-cell aggregation and intraspecies gene transfer. | enterococcus faecalis is an opportunistic pathogen that ranks among the leading causes of biofilm-associated infections. we previously demonstrated that the endocarditis- and biofilm-associated pili (ebp) of e. faecalis play a major role in biofilm formation, adherence to abiotic surfaces and experimental infections. in this study, derivatives of e. faecalis strain og1 were engineered to further characterize functions of ebp pili. loss of pili resulted in a 36-fold decrease in the number of clos ... | 2016 | 26967674 |
| point-of-use water disinfection using ultraviolet and visible light-emitting diodes. | improvements in point-of-use (pou) drinking water disinfection technologies for remote and regional communities are urgently needed. conceptually, uv-c light-emitting diodes (leds) overcome many drawbacks of low-pressure mercury tube based uv devices, and uv-a or visible light leds also show potential. to realistically evaluate the promise of led disinfection, our study assessed the performance of a model 1.3 l reactor, similar in size to solar disinfection bottles. in all, 12 different commerci ... | 2016 | 26967007 |
| bactericidal efficacy of cold plasma at different depths of infected root canals in vitro. | cold plasma (cp) has been shown to be effective even against multiresistant microorganisms. as previous investigations on the effect of cp in root canals showed promising results, the aim of the present study was to analyze the bactericidal efficacy of cp in different depths of infected dentin. | 2015 | 26962378 |
| heat-killed enterococcus faecalis ef-2001 ameliorates atopic dermatitis in a murine model. | recent reports have shown the immunomodulatory effect of heat-killed lactic acid bacteria. atopic dermatitis (ad) is an allergic skin disease, caused by immune dysregulation among other factors. the aim of this study was to assess the effect of heat-killed enterococcus faecalis ef-2001 (ef-2001) on ad. we established an in vivo ad model by repeated local exposure of dermatophagoides farinae extract (dfe; house dust mite extract) and 2,4-dinitrochlorobenzene (dncb) to the ears of mice. after oral ... | 2016 | 26959058 |
| antibacterial activities of lactic acid bacteria isolated from cow faeces against potential enteric pathogens. | the addition of sub therapeutic doses of antibiotics to cattle feed for growth promotion is a contributory factor to antibiotic resistance, thus an alternative to antibiotics is needed in animal feed additives. | 2015 | 26957978 |
| identification and prevalence of tetracycline resistance in enterococci isolated from poultry in ilishan, ogun state, nigeria. | tetracycline is one of the most frequently used antibiotics in nigeria both for human and animal infections because of its cheapness and ready availability. the use of tetracycline in animal husbandry could lead to horizontal transfer of tet genes from poultry to human through the gut microbiota, especially enterococci. therefore, this study is designed to identify different enterococcal species from poultry feces in selected farms in ilishan, ogun state, nigeria, determine the prevalence of tet ... | 2017 | 26957873 |
| effectiveness of n-acetyl cysteine, 2% chlorhexidine, and their combination as intracanal medicaments on enterococcus faecalis biofilm. | the purpose of this study was to evaluate the antibacterial efficacies of 2% chlorhexidine (chx), n-acetyl cysteine (nac) and assess their synergistic or antagonist action as intracanal medicament. | 2017 | 26957787 |
| molecular diagnosis of urinary tract infections by semi-quantitative detection of uropathogens in a routine clinical hospital setting. | the objective of our study was the development of a semi-quantitative real-time pcr to detect uropathogens. two multiplex pcr reactions were designed to detect escherichia coli, klebsiella spp., enterobacter spp., citrobacter spp., proteus mirabilis, enterococcus faecalis, and pseudomonas aeruginosa. 16s based pcr was performed in parallel to detect gram-positive and gram-negative bacteria. firstly to identify non-targeted agents of infection in the same urine specimen, and secondly to quantify ... | 2016 | 26954694 |
| co-location of the oxazolidinone resistance genes optra and cfr on a multiresistance plasmid from staphylococcus sciuri. | to identify and characterize the oxazolidinone/phenicol resistance gene optra in staphylococcus isolates. | 2016 | 26953332 |
| resistance to antimicrobial agents among enterococci isolated from fecal samples of wild marine species in the southern coast of brazil. | the purpose of this study was to evaluate species distribution, antimicrobial resistance profiles, and presence of resistance genes in enterococci isolated from fecal samples of wild marine species, including seabirds (n=12), sea turtles (n=8), and mammals (n=3) found alive or dead in southern coast of brazil. enterococci were classified based on phenotypic and genotypic characteristics, tested for antibiotic susceptibility, and the presence of tet(s), tet(m), tet(l), mrsc, and erm(b) genes by p ... | 2016 | 26952995 |
| cysteamine enhances biofilm eradication efficacy of calcium hydroxide. | calcium hydroxide (ca[oh]2) is a widely used interappointment dressing, but its antibacterial property is compromised by dentin. hence, the addition of chlorhexidine (chx) with ca(oh)2 has been proposed. however, the antimicrobial efficacy of this mixture compared with ca(oh)2 alone is currently still debatable. cysteamine is a mucolytic agent used to reduce the viscosity of mucus through the disruption of proteins, which are also important components of the extracellular matrix of biofilms. the ... | 2016 | 26951960 |
| effects of dentin debris on the antimicrobial properties of sodium hypochlorite and etidronic acid. | the purpose of this study was to determine the influence of dentin powder on the concentration, ph, and antimicrobial activity of sodium hypochlorite (naocl) alone and combined with etidronic acid (hebp). | 2016 | 26951957 |
| ppgpp negatively impacts ribosome assembly affecting growth and antimicrobial tolerance in gram-positive bacteria. | the stringent response is a survival mechanism used by bacteria to deal with stress. it is coordinated by the nucleotides guanosine tetraphosphate and pentaphosphate [(p)ppgpp], which interact with target proteins to promote bacterial survival. although this response has been well characterized in proteobacteria, very little is known about the effectors of this signaling system in gram-positive species. here, we report on the identification of seven target proteins for the stringent response nuc ... | 2016 | 26951678 |
| comparative analysis of protaper and waveone systems to reduce enterococcus faecalis from root canal system in primary molars--an in vitro study. | to assess, in vitro, the ability of the protaper(™) and waveone(™) systems to reduce enterococcus faecalis contamination in primary molars. | 2016 | 26950813 |
| the comparative evaluation of the antimicrobial effect of propolis with chlorhexidine against oral pathogens: an in vitro study. | this study aimed to compare the antimicrobial effectiveness of ethanolic extract of propolis (eep) to chlorhexidine gluconate (chx) on planktonic streptococcus mutans, streptococcus sobrinus, lactobacillus acidophilus, lactobacillus salivarius subsp. salivarius, aggregatibacter actinomycetemcomitans, prevotella intermedia, porphyromonas gingivalis, staphylococcus aureus, enterococcus faecalis, actinomyces israelii, candida albicans, and their single-species biofilms by agar dilution and broth mi ... | 2016 | 26949701 |
| in silico dissection of type vii secretion system components across bacteria: new directions towards functional characterization. | type vii secretion system (t7ss) is one of the factors involved in virulence of mycobacterium tuberculosis h37rv. numerous research efforts have been made in the last decade towards characterizing the components of this secretion system. an extensive genome-wide analysis through compilation of isolated information is required to obtain a global view of diverse characteristics and pathogenicity-related aspects of this machinery. the present study suggests that differences in structural components ... | 2016 | 26949095 |
| invasive enterococcal infections in poland: the current epidemiological situation. | the aim of this study was to investigate human invasive isolates of enterococci, obtained through prospective surveillance in poland. the consecutive enterococcal isolates were collected in 30 hospitals between may 2010 and june 2011, and studied by species identification, antimicrobial susceptibility testing and, for enterococcus faecium by detection of markers specific for the hospital meroclone, multilocus vntr analysis (mlva) and multilocus sequence typing (mlst). additionally, the genomic d ... | 2016 | 26946510 |
| antibacterial and cytotoxic triterpenoids from the roots of combretum racemosum. | a new pentacyclic triterpenoid glucoside, together with fourteen known compounds, was isolated from the roots of combretum racemosum. combretaceae). the structure of the new compound was established as 28-o-β-d-glucopyranosyl-2α,3β,21β,23-tetrahydroxyolean-18-en-28-oate (1) on the basis of detailed spectroscopic data including ms, 1d, and 2d nmr. the inhibitory activity of compounds 1-15 against promyelocytic leukemia hl-60 and human erythromyeloblastoid leukemia k562 cell lines was evaluated. c ... | 2016 | 26946378 |
| time is of the essence: the impact of delayed antibiotic therapy on patient outcomes in hospital-onset enterococcal bloodstream infections. | with increasing prevalence of vancomycin-resistant enterococci (vre), appropriate antibiotic therapy for enterococcal bloodstream infections (ebsi) can be delayed. data regarding the impact of delayed therapy on ebsi outcomes are conflicting, and the time delay most strongly associated with poor outcomes has not been defined. | 2016 | 26945013 |
| occurrence of vancomycin-resistant and -susceptible enterococcus spp. in reclaimed water used for spray irrigation. | reclaiming municipal wastewater for agricultural, environmental, and industrial purposes is increasing in the united states to combat dwindling freshwater supplies. however, there is a lack of data regarding the microbial quality of reclaimed water. in particular, no previous studies have evaluated the occurrence of vancomycin-resistant enterococci (vre) in reclaimed water used at spray irrigation sites in the united states. to address this knowledge gap, we investigated the occurrence, concentr ... | 2016 | 26942838 |
| antimicrobial activity of herbal medicines (tulsi extract, neem extract) and chlorhexidine against enterococcus faecalis in endodontics: an in vitro study. | successful endodontic treatment depends on effective disinfection and complete sealing of root canal. various medicaments are advised for disinfecting root canal, such as herbal and non-herbal medicaments. this study was done to assess the antimicrobial activity of herbal medicines (neem extract, tulsi extract) and chlorhexidine against enterococcus faecalis in endodontics. | 2015 | 26942123 |
| killing of vre enterococcus faecalis by commensal strains: evidence for evolution and accumulation of mobile elements in the absence of competition. | enterococci are members of the gastrointestinal tract of humans and most animals that, over the past 3 decades, have emerged as leading causes of multidrug resistant hospital acquired infection (hai). in addition to their general hardiness, many traits have entered enterococcal lineages through horizontal gene transfer, which has led to the evolution of pathogenic hospital-associated lineages uniquely adapted for survival and proliferation in the antibiotic perturbed ecology of the gastrointesti ... | 2016 | 26939857 |
| comparative antimicrobial efficacy of herbal alternatives (emblica officinalis, psidium guajava), mtad, and 2.5% sodium hypochlorite against enterococcus faecalis: an in vitro study. | the objective of this study was to evaluate the antimicrobial efficacy of herbal alternatives (emblica officinalis, psidium guajava), biopure mtad, and 2.5% sodium hypochlorite against enterococcus faecalis. | 2017 | 26937369 |
| in vitro antimicrobial evaluation of toothpastes with natural compounds. | this in vitro study evaluated the antimicrobial effects of commercial toothpastes containing natural compounds. | 2017 | 26929699 |
| ampicillin in combination with ceftaroline, cefepime, or ceftriaxone demonstrates equivalent activities in a high-inoculum enterococcus faecalis infection model. | ampicillin-ceftriaxone combination therapy has become a predominant treatment for serious enterococcus faecalis infections, such as endocarditis. unfortunately, ceftriaxone use is associated with future vancomycin-resistant enterococcus colonization. we evaluated e. faecalis in an in vitro pharmacodynamic model against simulated human concentration-time profiles of ampicillin plus ceftaroline, cefepime, ceftriaxone, or gentamicin. ampicillin-cefepime and ampicillin-ceftaroline demonstrated activ ... | 2016 | 26926624 |
| chemical composition and antimicrobial activity of the essential oil and methanol extract of hypericum aegypticum subsp. webbii (spach) n. robson. | hypericum aegypticum subsp. webbii is an evergreen shrub spread in mediterranean part of central and southeastern europe. the chemical composition and antimicrobial activity of the essential oil and meoh extract of h. aegypticum subsp. webbii were investigated. the monoterpenes α-pinene (63.4 - 68.5%) and β-pinene (16.9 - 17.0%) were main compounds in the volatile oil from aerial parts. in the cluster analysis, the essential oil of h. aegypticum subsp. webbii was separated and chemically differe ... | 2016 | 26923468 |
| synthesis of newer 1,2,3-triazole linked chalcone and flavone hybrid compounds and evaluation of their antimicrobial and cytotoxic activities. | the present study was carried out in an attempt to synthesize a new class of antimicrobial and antiplasmodial agents by copper catalyzed click chemistry to afford 25 compounds 10-14(a-e) of 1,4-disubstituted-1,2,3-triazole derivatives of chalcones and flavones. the structures of the newly synthesized compounds were established by elemental analysis, ir, (1)h nmr, (13)c nmr and mass spectral data. the newly synthesized compounds were evaluated for their antibacterial activity against gram positiv ... | 2016 | 26922227 |
| isolation and structural elucidation of brevibacillin, an antimicrobial lipopeptide from brevibacillus laterosporus that combats drug-resistant gram-positive bacteria. | a new environmental bacterial strain exhibited strong antimicrobial characteristics against methicillin-resistant staphylococcus aureus, vancomycin-resistant strains of enterococcus faecalis and lactobacillus plantarum, and other gram-positive bacteria. the producer strain, designated osy-i1, was determined to be brevibacillus laterosporusvia morphological, biochemical, and genetic analyses. the antimicrobial agent was extracted from cells of osy-i1 with isopropanol, purified by high-performance ... | 2016 | 26921428 |
| lipoteichoic acid of enterococcus faecalis inhibits the differentiation of macrophages into osteoclasts. | enterococcus faecalis is associated with persistent endodontic infection and refractory apical periodontitis. recently, we have shown that heat-killed e. faecalis attenuates osteoclast differentiation. because lipoteichoic acid (lta) is a major virulence factor of gram-positive bacteria, we investigated the effect of lta from e. faecalis (eflta) on osteoclast differentiation. | 2016 | 26920932 |
| envelope structures of gram-positive bacteria. | gram-positive organisms, including the pathogens staphylococcus aureus, streptococcus pneumoniae, and enterococcus faecalis, have dynamic cell envelopes that mediate interactions with the environment and serve as the first line of defense against toxic molecules. major components of the cell envelope include peptidoglycan (pg), which is a well-established target for antibiotics, teichoic acids (tas), capsular polysaccharides (cps), surface proteins, and phospholipids. these components can underg ... | 2016 | 26919863 |
| the impact of host metapopulation structure on the population genetics of colonizing bacteria. | many key bacterial pathogens are frequently carried asymptomatically, and the emergence and spread of these opportunistic pathogens can be driven, or mitigated, via demographic changes within the host population. these inter-host transmission dynamics combine with basic evolutionary parameters such as rates of mutation and recombination, population size and selection, to shape the genetic diversity within bacterial populations. whilst many studies have focused on how molecular processes underpin ... | 2016 | 26916623 |
| synthesis and biological activities of lanthanide (iii) nitrate complexes with n-(2-hydroxynaphthalen-1-yl) methylene) nicotinohydrazide schiff base. | the field of coordination chemistry has registered a phenomenal growth during last few decades. it is well known that precious metals have been used for medicinal purposes for at least 3500 years. at that time, precious metals were believed to benefit health because of their rarity, but research has now well established the link between medicinal properties of inorganic drugs and specific biological properties. | 2016 | 26916500 |
| in vitro antibacterial activity of a silicone-based endodontic sealer and two conventional sealers. | the aim of this study was to evaluate whether the modification in the silver component is capable of providing guttaflow 2 with antibacterial activity against enterococcus faecalis compared with epoxy resin-based (ah plus) and zinc oxide and eugenol-based (endofill) sealers. the antibacterial activity was evaluated using a reference strain of e. faecalis (atcc 29212). freshly mixed sealers were subjected to the agar diffusion test (adt), while the direct contact test (dct) was performed after ma ... | 2016 | 26910015 |
| screening of the enterocin-encoding genes and antimicrobial activity in enterococcus species. | in the current study, a total of 135 enterococci strains from different sources were screened for the presence of the enterocin-encoding genes enta, entp, entb, entl50a, and entl50b. the enterocin genes were present at different frequencies, with enta occurring the most frequently, followed by entp and entb; entl50a and l50b were not detected. the occurrence of single enterocin genes was higher than the occurrence of multiple enterocin gene combinations. the 80 isolates that harbor at least one ... | 2016 | 26907753 |
| prevalence and risk factors of early fecal carriage of enterococcus faecalis and staphylococcus spp and their antimicrobial resistant patterns among healthy neonates born in a hospital setting in central saudi arabia. | to investigate the prevalence, antibiotic resistant profiles, and risk factors of early fecal carriage of enterococcus faecalis (e. faecalis) and staphylococci among 150 healthy saudi neonates born in a hospital setting in central saudi arabia. | 2016 | 26905350 |
| evolution of resistance to cationic biocides in streptococcus mutans and enterococcus faecalis. | the aim of this study was to investigate whether streptococcus mutans and enterococcus faecalis develop resistance to the cationic biocides chlorhexidine (chx), cetylpyridinium chloride (cpc), and 12-methacryloyloxydodecylpyridinium bromide (mdpb). | 2016 | 26904979 |
| study of the antibacterial efficacy of bainiku-ekisu against pathogens. | the research was undertaken to determine the bacteriostatic effects of the concentrate of japanese apricot juice (bainiku-ekisu), which is a popular health food in taiwan and japan, on enterococcus faecalis atcc 29212, staphylococcus aureus atcc 25923, and escherichia coli atcc 25922. the results show that e. faecalis, s. aureus, and e. coli could be killed or inhibited by bainiku-ekisu at concentrations between 1.0 and 10.0 mg/ml. the minimum inhibitory concentration (mic) was 1 mg/ml for all s ... | 2014 | 26904733 |
| functional analysis of the citrate activator cito from enterococcus faecalis implicates a divalent metal in ligand binding. | the regulator of citrate metabolism, cito, from enterococcus faecalis belongs to the fcd family within the gntr superfamily. in the presence of citrate, cito binds to cis-acting sequences located upstream of the cit promoters inducing the expression of genes involved in citrate utilization. the quantification of the molecular binding affinities, performed by isothermal titration calorimetry (itc), indicated that cito has a high affinity for citrate (k d = 1.2 ± 0.2 μm), while it did not recogniz ... | 2016 | 26903980 |
| genetic environment of the transferable oxazolidinone/phenicol resistance gene optra in enterococcus faecalis isolates of human and animal origin. | aim of this study was to analyse 17 non-related enterococcus faecalis isolates of human and animal origin for the genetic environment of the novel oxazolidinone/phenicol resistance gene optra. | 2016 | 26903276 |
| evaluation of polymorphisms in pbp4 gene and genetic diversity in penicillin-resistant, ampicillin-susceptible enterococcus faecalis from hospitals in different states in brazil. | the aim of the present study was to verify whether penicillin-resistant, ampicillin-susceptible enterococcus faecalis (prasef) occurred in brazil prior to the beginning of the 21st century, and to verify whether ampicillin susceptibility can predict susceptibility to other β-lactams in e. faecalis with this inconsistent phenotype. the presence of polymorphisms in the pbp4 gene and genetic diversity among the isolates were investigated. of 21 prasef analyzed, 5 (23.8%) and 4 (19.0%) were imipenem ... | 2016 | 26903013 |
| clinical characteristics and antimicrobial patterns in complicated intra-abdominal infections: a 6-year epidemiological study in southern china. | complicated intra-abdominal infection (ciais) are a common and important cause of morbidity worldwide. in this study, the clinical features, microbiological profiles, antimicrobial patterns and treatments of 3233 ciai patients (mean age, 47.6 years; 54.7% male) with 3531 hospitalisations from 2008-2013 were retrospectively investigated. the most commonly isolated bacteria were escherichia coli (47.6%), klebsiella pneumoniae (16.9%), enterococcus faecalis (10.4%) and pseudomonas aeruginosa (8.8%) ... | 2016 | 26899413 |
| comparison of telavancin and vancomycin lock solutions in eradication of biofilm-producing staphylococci and enterococci from central venous catheters. | results of a study of the activity of antibiotic lock solutions of vancomycin and telavancin against biofilm-forming strains of staphylococcus epidermidis, enterococcus faecalis, and staphylococcus aureus are reported. | 2016 | 26896504 |
| addressing the requirements of high-sensitivity single-molecule imaging of low-copy-number proteins in bacteria. | single-molecule fluorescence super-resolution imaging and tracking provide nanometer-scale information about subcellular protein positions and dynamics. these single-molecule imaging experiments can be very powerful, but they are best suited to high-copy number proteins where many measurements can be made sequentially in each cell. we describe artifacts associated with the challenge of imaging a protein expressed in only a few copies per cell. we image live bacillus subtilis in a fluorescence mi ... | 2016 | 26888309 |
| [clinical observations of acute-onset endophthalmitis after clear corneal phacoemulsification]. | to evaluate the incidence of acute-onset endophthalmitis after clear corneal phacoemulsification and to report its clinical features, microbiology, and therapeutic efficacy. | 2015 | 26888274 |
| to comparatively evaluate the antimicrobial efficacy of chlorhexidine, nisin and linezolid as an intracanal medicament on enterococcus faecalis: an in vitro study. | the present study evaluated the antimicrobial efficacy of chlorhexidine, nisin and linezolid and a control group (normal saline ) against enterococcus faecalis (ef). | 2016 | 26888241 |
| the influence of urinary tract infections on treatment of fracture of proximal femur in patients with systemiv osteoporosis. | in the structure of intrahospital infections the infections of urinary tract make up from 20 to 40%. this problem represents special medical-social significance for trauma departments as patients with trauma are being in forced situation for along time. 380 patients older than 60 years (average age 73.6 ± 9.2 years) with a fracture of proximal femur against background of the systemic osteoporosis having in-patient treatment in the trauma department of the republic hospital no2 were being examine ... | 2015 | 26887137 |
| a new mechanism of post-transfer editing by aminoacyl-trna synthetases: catalysis of hydrolytic reaction by bacterial-type prolyl-trna synthetase. | aminoacyl trna synthetases are enzymes that specifically attach amino acids to cognate trnas for use in the ribosomal stage of translation. for many aminoacyl trna synthetases, the required level of amino acid specificity is achieved either by specific hydrolysis of misactivated aminoacyl-adenylate intermediate (pre-transfer editing) or by hydrolysis of the mischarged aminoacyl-trna (post-transfer editing). to investigate the mechanism of post-transfer editing of alanine by prolyl-trna synthetas ... | 2017 | 26886480 |
| antibacterial effect of hydroalcoholic extract of punica granatum linn. petal on common oral microorganisms. | objectives. this study aimed to assess the effect of hydroalcoholic extract of punica granatum linn. (p. granatum) petal on streptococcus sanguinis, streptococcus mutans, streptococcus salivarius, streptococcus sobrinus, and enterococcus faecalis. materials and methods. in this in vitro study, p. granatum extract was prepared using powdered petals and water-ethanol solvent. antibacterial effect of the extract, chlorhexidine (chx), and ampicillin was evaluated on brain heart infusion agar (bhia) ... | 2016 | 26884763 |
| functional dissection of the crors two-component system required for resistance to cell wall stressors in enterococcus faecalis. | bacteria use two-component signal transduction systems (tcss) to sense and respond to environmental changes via a conserved phosphorelay between a sensor histidine kinase and its cognate response regulator. the opportunistic pathogen enterococcus faecalis utilizes a tcs comprised of the histidine kinase cros and the response regulator cror to mediate resistance to cell wall stresses such as cephalosporin antibiotics, but the molecular details by which crors promotes cephalosporin resistance have ... | 2016 | 26883822 |
| bacteriospermia and its impact on basic semen parameters among infertile men. | introduction. semen analysis is considered as the surrogate marker for male fecundity while assessing infertile men. there are several reasons for altered semen quality and bacteriospermia could be one among them. thereby the aim of our work is to study the semen culture and its impact on semen parameters among infertile men. materials and methods. semen samples were collected from men attending infertility clinic. semen parameters were analysed based on who guidelines. also, samples were subjec ... | 2016 | 26880908 |
| electrochemical disinfection of simulated ballast water on pbo2/graphite felt electrode. | a novel pbo2/graphite felt electrode was constructed by electrochemical deposition of pbo2 on graphite felt and characterized by x-ray powder diffraction (xrd) and scanning electron microscopy (sem) analysis. the prepared electrode is a viable technology for inactivation of escherichia coli, enterococcus faecalis, and artemia salina as indicator organisms in simulated ballast water treatment, which meets the international maritime organization (imo) regulation d-2. the effects of contact time an ... | 2016 | 26880130 |
| isoflavone metabolism by a collection of lactic acid bacteria and bifidobacteria with biotechnological interest. | almost all soy isoflavones exist as glycosides, daidzin, genistin, and glycitin. we analyzed the capacity of 92 strains of lactic acid bacteria (lab) and bifidobacteria with biotechnological interest to process the glycosylated isoflavones daidzin, genistin, and glycitin in their more bioavailable aglycones and their metabolites as dihydrodaidzein (dhd), o-desmethylangolensin, and equol. representative strains of the four genera studied lactobacillus, enterococcus, lactococcus, and bifidobacteri ... | 2016 | 26878882 |
| cholesterol suppresses antimicrobial effect of statins. | isoprenoid biosynthesis is a key metabolic pathway to produce a wide variety of biomolecules such as cholesterol and carotenoids, which target cell membranes. on the other hand, it has been reported that statins known as inhibitors of isoprenoid biosynthesis and cholesterol lowering agents, may have a direct antimicrobial effect on the some bacteria. the exact action of statins in microbial metabolism is not clearly understood. it is possible that statins inhibit synthesis or utilization of some ... | 2015 | 26877857 |
| purification and characteristics of a novel bacteriocin produced by enterococcus faecalis l11 isolated from chinese traditional fermented cucumber. | to purify and characterize a novel bacteriocin with broad inhibitory spectrum produced by an isolate of enterococcus faecalis from chinese fermented cucumber. | 2016 | 26875092 |
| photodynamic uva-riboflavin bacterial elimination in antibiotic-resistant bacteria. | to evaluate the bactericidal effect of clinical ultraviolet a (uva) settings used in photoactivated chromophore for infectious keratitis (pack)-collagen cross-linking (cxl) in antibiotic-resistant and non-resistant bacterial strains. | 2016 | 26867998 |
| composition and dynamics of the respiratory tract microbiome in intubated patients. | lower respiratory tract infection (lrti) is a major contributor to respiratory failure requiring intubation and mechanical ventilation. lrti also occurs during mechanical ventilation, increasing the morbidity and mortality of intubated patients. we sought to understand the dynamics of respiratory tract microbiota following intubation and the relationship between microbial community structure and infection. | 2016 | 26865050 |
| enterococcus faecium small colony variant endocarditis in an immunocompetent patient. | small colony variants (scv) are slow-growing subpopulations of bacteria usually associated with auxotrophism, causing persistent or recurrent infections. enterococcus faecalis scv have been seldom described, and only one case of enterococcus faecium scv has been reported, associated with sepsis in a leukaemia patient. here we report the first case described of bacteraemia and endocarditis by scv e. faecium in an immunocompetent patient. | 2016 | 26862434 |
| antibiofilm efficacy of honey and bee-derived defensin-1 on multi-species wound biofilm. | many clinically relevant biofilms are polymicrobial. examining the effect of antimicrobials in a multi-species biofilm consortium is of great clinical importance. the goal of this study was to investigate the effect of different honey types against bacterial wound pathogens grown in multi-species biofilm and to test the antibiofilm activity of honey defensin-1 in its recombinant form. modified lubbock chronic wound biofilm formed by four bacterial species (staphylococcus aureus, streptococcus ag ... | 2016 | 26861950 |
| field study on evaluation of the efficacy and usability of two disinfectants for drinking water treatment at small cattle breeders and dairy cattle farms. | the hygienic quality of drinking water for cattle originated from different sources together with the efficacy and usability of two types of disinfectants against waterborne pathogens were assessed for small cattle breeders and dairy cattle farms. a total of 120 drinking water samples were collected from water troughs representing three different water sources commonly used for cattle drinking (tap, underground and surface water; n = 65, 25, and 30, respectively). collected samples were cultured ... | 2016 | 26861741 |
| photoactivated disinfection (pad) of dental root canal system - an ex-vivo study. | to investigate the efficacy of photo activated disinfection (pad) in reducing colony-forming unit (cfu) counts of enterococcus faecalis (e. faecalis) in infected dental root canals. the study compared the efficacy of pad with conventional endodontic treatment (cet) and also a combination of cet along with pad. | 2016 | 26858548 |
| [in vitro activity of ampicillin-ceftriaxone against enterococcus faecalis isolates recovered from invasive infections]. | in vitro activity of the combination of ampicillin- ceftriaxone against 30 enterococcus faecalis isolates recovered from invasive infections in patients admitted to hospital de clínicas josé de san martin in the city of buenos aires was assessed. ampicillin- ceftriaxone synergies were determined by microdilution in müeller-hinton (mh) broth with and without subinhibitory concentrations of ceftriaxone. synergy was detected in 22/30 isolates. a decrease in both minimum inhibitory concentration (mi ... | 2016 | 26857425 |
| an analysis in vivo of intracanal bacterial load before and after chemo-mechanical preparation: a comparative analysis of two irrigants and two activation techniques. | the goals of this randomized double-blind trial were to assess the antimicrobial activity in vivo of sodium hypochlorite (naocl) vs. chlorhexidine gluconate (chx) used in combination either with endoactivator® or irri s® files in patients with apical periodontitis. | 2016 | 26855714 |
| the effects of silver nanoparticles on antimicrobial activity of proroot mineral trioxide aggregate (mta) and calcium enriched mixture (cem). | although, mineral trioxide aggregate (mta) and new experimental cement (cem) are good root filling cements, but had no or low antimicrobial activities. the aim of this study was to evaluate the effects of addition of silver nanoparticles (snp) to these two cements on antimicrobial effects against five most dental infection related microorganisms. | 2016 | 26855701 |
| gut-colonizing bacteria promote c. elegans innate immunity by producing nitric oxide. | many commensal bacteria in the gut are beneficial to the host immune system, but the underlying mechanisms are largely unclear. using culture-independent illumina miseq sequencing of the bacterial 16s rrna gene amplicons, we show that bacterial diversity in the intestine of caenorhabditis elegans, the free-living nematode, is distinct from that in soil. of these bacteria, bacillus subtilis is the most prominent species in the worm gut. we demonstrate that b. subtilis confers worm resistance to i ... | 2016 | 26854225 |
| did the iceman know better? screening of the medicinal properties of the birch polypore medicinal mushroom, piptoporus betulinus (higher basidiomycetes). | the birch polypore piptoporus betulinus was among two mushrooms that were found in the iceman's bag. recent studies indicated that p. betulinus was probably used as a religious and medicinal item. in order to examine the medicinal potential of p. betulinus, hot water (hw), partially purified (pp), and alkali extract (ha) were prepared and tested for antioxidant, antimicrobial, cytotoxic, and angiotensin i-converting enzyme (ace) inhibitory activity. all tested samples exhibited moderate cytotoxi ... | 2015 | 26854098 |
| incidence of virulence determinants in clinical enterococcus faecalis and enterococcus faecium isolates collected in bulgaria. | to evaluate the prevalence of some virulence genes among 510 clinical enterococcus spp. isolates and to assess the association of those genes with the species, infection site, and patient group (inpatients/outpatients). | 2016 | 26849965 |
| epigenetic activation of antibacterial property of an endophytic streptomyces coelicolor strain azra 37 and identification of the induced protein using maldi tof ms/ms. | the endophytic streptomyces coelicolor strain azra 37 was isolated from the surface sterilized root of azadirachta indica a. juss., commonly known as neem plant in india. since only a few reports are available regarding epigenetic modulations of microbial entities, s. coelicolor was treated with different concentrations of 5-azacytidine for this purpose and evaluated for its antibacterial potential against five human pathogenic bacteria (aeromonas hydrophila ims/gn11, enterococcus faecalis ims/g ... | 2016 | 26844762 |
| antimicrobial and cytotoxic activity of cuminum cyminum as an intracanal medicament compared to chlorhexidine gel. | the aims of this study were i) to define the chemical constituents of cuminum cyminum (cumin) essential oil, ii) to compare the antimicrobial activity of this oil to that of chlorhexidine (chx) and co-trimoxazole on planktonic and biofilm forms of bacteria isolated from the teeth with persistent endodontic infection and iii ) to compare the cytotoxicity of these medicaments on l929 fibroblasts. | 2016 | 26843877 |
| antibacterial activity of diode laser and sodium hypochlorite in enterococcus faecalis-contaminated root canals. | the aim of the present in vitro study was to evaluate the disinfection ability of 980-nm diode laser in comparison with sodium hypochlorite (naocl) as a common root canal irrigant in canals infected with enterococcus faecalis (e. faecalis). | 2016 | 26843870 |
| dental unit water treatment with hydrogen peroxide and monovalent silver ions artificially contaminated with freshly isolated pathogens. | dental unit water (duw) could be contaminated by human pathogens coming from biological fluids penetrated during patient treatment and by opportunistic pathogens detached from aquatic biofilm. these microorganisms could be spread to following patients. we tested the disinfectant activity of hydrogen peroxide and monovalent silver ions (h(2)o(2)-ag(+)) into duw artificially contaminated with freshly isolated pathogens. | 2016 | 26835793 |
| expression of alzheimer-type neurofibrillary epitopes in primary rat cortical neurons following infection with enterococcus faecalis. | the neurofibrillary tau pathology and amyloid deposits seen in alzheimer's disease (ad) also have been seen in bacteria-infected brains. however, few studies have examined the role of these bacteria in the generation of tau pathology. one suggested link between infection and ad is edentulism, the complete loss of teeth. edentulism can result from chronic periodontal disease due to infection by enterococcus faecalis. the current study assessed the ability to generate early alzheimer-like neurofib ... | 2015 | 26834627 |
| effectiveness of different disinfection protocols on the reduction of bacteria in enterococcus faecalis biofilm in teeth with large root canals. | this study compared the effectiveness of different disinfection protocols in reducing bacteria in an enterococcus faecalis biofilm in teeth with large root canals. | 2016 | 26830428 |
| fibrinogen release and deposition on urinary catheters placed during urological procedures. | catheter associated urinary tract infections account for approximately 40% of all hospital acquired infections worldwide with more than 1 million cases diagnosed annually. recent data from a catheter associated urinary tract infection animal model has shown that inflammation induced by catheterization releases host fibrinogen, which accumulates on the catheter. further, enterococcus faecalis catheter colonization was found to depend on ebpa (endocarditis and biofilm-associated pilus), a fibrinog ... | 2016 | 26827873 |
| cardiac surgery for infective endocarditis in patients with intravenous drug use. | intravenous drug users have a high risk of infective endocarditis and reduced survival. cardiac surgery may be recommended for these patients, but redo surgery is controversial. this study describes the characteristics and outcomes of intravenous drug users accepted for surgery during a 12-year period. | 2016 | 26826713 |
| γ-cyclodextrin capped silver nanoparticles for molecular recognition and enhancement of antibacterial activity of chloramphenicol. | computational studies were conducted to identify the favourable formation of the inclusion complex of chloramphenicol with cyclodextrins. the results of molecular docking and molecular dynamics predicted the strongest interaction of chloramphenicol with γ-cyclodextrin. further, the inclusion complex of chloramphenicol with γ-cyclodextrin was experimentally prepared and a phenomenon of inclusion was verified by using different characterization techniques such as thermogravimetric analysis, differ ... | 2016 | 26824520 |
| cloning and purification of the first termicin-like peptide from the cockroach eupolyphaga sinensis. | termicin is an antimicrobial peptide with six cysteines forming three disulfide bridges that was firstly isolated from the salivary glands and hemocytes of the termite pseudacanthotermes spiniger. in contrast to many broad-spectrum antimicrobial peptides, termicin is most active against filamentous fungi. although more than one hundred complementary dnas (cdnas) encoding termicin-like peptides have been reported to date, all these termicin-like peptides were obtained from isoptera insects. | 2016 | 26823660 |
| development and characterization of potent cyclic acyldepsipeptide analogues with increased antimicrobial activity. | the problem of antibiotic resistance has prompted the search for new antibiotics with novel mechanisms of action. analogues of the a54556 cyclic acyldepsipeptides (adeps) represent an attractive class of antimicrobial agents that act through dysregulation of caseinolytic protease (clpp). previous studies have shown that adeps are active against gram-positive bacteria (e.g., mrsa, vre, prsp (penicillin-resistant streptococcus pneumoniae)); however, there are currently few studies examining gram-n ... | 2016 | 26818454 |
| [the incidence and risk factors of male urinary tract infection following invasive urodynamic investigation]. | to assess the prevalence of male urinary tract infection (uti) after invasive urodynamic studies and to determinate the risk factors by evaluating the clinical characteristics and urodynamic parameters of patients. | 2015 | 26813139 |
| gut microbiota imbalance and colorectal cancer. | the gut microbiota acts as a real organ. the symbiotic interactions between resident micro-organisms and the digestive tract highly contribute to maintain the gut homeostasis. however, alterations to the microbiome caused by environmental changes (e.g., infection, diet and/or lifestyle) can disturb this symbiotic relationship and promote disease, such as inflammatory bowel diseases and cancer. colorectal cancer is a complex association of tumoral cells, non-neoplastic cells and a large amount of ... | 2016 | 26811603 |
| inhibition of bacterial and fungal pathogens by the orphaned drug auranofin. | we identified auranofin as an antimicrobial compound utilizing a high-throughput screen using a caenorhabditis elegans-staphylococcus aureus infection model. results/methodology: treatment of infected nematodes with auranofin resulted in a prolonged survival rate of 95%, reached with 0.78 μg/ml. further investigation of the antimicrobial activity of auranofin found inhibition against s. aureus, enterococcus faecium and enterococcus faecalis. importantly, the fungal pathogens cryptococcus neoform ... | 2016 | 26808006 |
| redefining healthy urine: a cross-sectional exploratory metagenomic study of people with and without bladder dysfunction. | we used the pathoscope platform to perform species level analyses of publicly available, 16s rrna pyrosequenced, asymptomatic urine data to determine relationships between microbiomes, and clinical and functional phenotypes. | 2016 | 26807926 |
| in vitro antimicrobial and anti-proliferative activities of plant extracts from spathodea campanulata, ficus bubu, and carica papaya. | african medicinal plants represent a prominent source of new active substances. in this context, three plants were selected for biological investigations based on their traditional uses. | 2016 | 26799575 |
| monitoring the effectiveness of photodynamic therapy with periodic renewal of the photosensitizer on intracanal enterococcus faecalis biofilms. | photodynamic therapy (pdt) can eliminate microorganisms in a root canal. however, the parameters for disinfection remain undefined. this study assessed the effectiveness of a pdt protocol against intracanal enterococcus faecalis biofilms. | 2016 | 26796030 |
| vinegar treatment prevents the development of murine experimental colitis via inhibition of inflammation and apoptosis. | this study investigated the preventive effects of vinegar and acetic acid (the active component of vinegar) on ulcerative colitis (uc) in mice. vinegar (5% v/v) or acetic acid (0.3% w/v) treatment significantly reduced the disease activity index and histopathological scores, attenuated body weight loss, and shortened the colon length in a murine experimental colitis model induced by dextran sulfate sodium (dss). further mechanistic analysis showed that vinegar inhibited inflammation through supp ... | 2016 | 26795553 |
| enterococcus faecalis from food, clinical specimens, and oral sites: prevalence of virulence factors in association with biofilm formation. | enterococci have gained significance as the cause of nosocomial infections; they occur as food contaminants and have also been linked to dental diseases. e. faecalis has a great potential to spread virulence as well as antibiotic resistance genes via horizontal gene transfer. the integration of food-borne enterococci into the oral biofilm in-vivo has been observed. therefore, we investigated the virulence determinants and antibiotic resistance of 97 e. faecalis isolates from the oral cavity, foo ... | 2015 | 26793174 |
| global regulation of gene expression by the mafr protein of enterococcus faecalis. | enterococcus faecalis is a natural inhabitant of the human gastrointestinal tract. however, as an opportunistic pathogen, it is able to colonize other host niches and cause life-threatening infections. its adaptation to new environments involves global changes in gene expression. the ef3013 gene (here named mafr) of e. faecalis strain v583 encodes a protein (mafr, 482 residues) that has sequence similarity to global response regulators of the mga/atxa family. the enterococcal og1rf genome also e ... | 2015 | 26793169 |
| evaluation of selected properties of a new root repair cement containing surface pre-reacted glass ionomer fillers. | this study evaluated selected properties of a prototype root repair cement containing surface pre-reacted glass ionomer fillers (s-prg) in comparison to mineral trioxide aggregate (mta) and intermediate restorative material (irm). | 2016 | 26787616 |
| partial purification and characterization of bacteriocin produced by enterococcus faecalis du10 and its probiotic attributes. | a novel bacteriocin produced by avian duck isolated lactic acid bacterium enterococcus faecalis du10 was isolated. this bacteriocin showed a broad spectrum of antibacterial activity against important food-borne pathogens and was purified by size exclusion chromatography followed by reverse-phase high-performance liquid chromatography in a c-18 column. tricine-sds page revealed the presence of a band with an estimated molecular mass of 6.3 kda. the zymogram clearly linked the antimicrobial activi ... | 2016 | 26786752 |
| a novel mva-mediated pathway for isoprene production in engineered e. coli. | to deal with the increasingly severe energy crisis and environmental consequences, biofuels and biochemicals generated from renewable resources could serve as a promising alternative for replacing petroleum as a source of fuel and chemicals, among which isoprene (2-methyl-1,3-butadiene) in particular is of great significance in that it is an important platform chemical, which has been used in industrial production of synthetic rubber for tires and coatings or aviation fuel. | 2016 | 26786050 |
| nitric oxide-releasing polyacrylonitrile disperses biofilms formed by wound-relevant pathogenic bacteria. | to test the antimicrobial and antibiofilm properties of a nitric oxide (no)-releasing polymer against wound-relevant bacterial pathogens. | 2016 | 26784529 |
| pseudomonas putida strain fstm2 isolated from shark skin: a potential source of bacteriocin. | bacteriocin-producing pseudomonas putida strain fstm2 isolated from shark showed broad range of antibacterial activity against all pathogens tested except bacillus subtilis atcc11774, mrsa n32064, proteus mirabilis atcc12453, enterococcus faecalis atcc14506, salmonella typhimurium atcc51312, salmonella mutan atcc25175, and aeromonas hydrophila wbf314. of the three growth media tested in this study, tsb was observed to support the bacteriocin activity the most. while the highest bacteriocin activ ... | 2013 | 26782985 |
| partial purification and characterization of a bacteriocin dt24 produced by probiotic vaginal lactobacillus brevis dt24 and determination of its anti-uropathogenic escherichia coli potential. | the emergence of antibiotic resistance has increased the interest for finding new antimicrobials in the past decade. probiotic lactic acid bacteria producing antimicrobial proteins like bacteriocin can be excellent agents for development as novel therapeutic agents and complement to conventional antibiotic therapy. uropathogenic escherichia coli, most causative agent of urinary tract infection, has developed resistance to various antibiotics. in the present investigation, antibacterial substance ... | 2013 | 26782739 |
| escherichia coli and enterococcus faecalis are able to incorporate and enhance a pre-formed gardnerella vaginalis biofilm. | gardnerella vaginalis is the most frequent microorganism found in bacterial vaginosis (bv), while escherichia coli and enterococcus faecalis are amongst the most frequent pathogens found in urinary tract infections (utis). this study aimed to evaluate possible interactions between utis pathogens and g. vaginalis using an in vitro dual-species biofilm model. our results showed that dual-species biofilms reached significantly higher bacterial concentration than monospecies biofilms. moreover, visu ... | 2016 | 26782142 |
| fluorescence-based comparative evaluation of bactericidal potency and food application potential of anti-listerial bacteriocin produced by lactic acid bacteria isolated from indigenous samples. | the aim of the present study was to ascertain the potency of anti-listerial bacteriocin produced by lactic acid bacteria (lab) isolated from indigenous samples of dahi, dried fish, and salt-fermented cucumber. a total of 231 lab isolates were obtained from the samples, of which 51 isolates displayed anti-listerial activity. the anti-listerial lab were identified by pcr as lactobacillus sp., pediococcus sp., enterococcus sp., and lactococcus sp. pcr also enabled the detection of class iia bacteri ... | 2012 | 26781854 |
| bacteriocin-related siblicide in clinical isolates of enterococci. | siblicide is a phenomenon defined in the present context as an enterococcus strain that, while growing as a colony on solid media, exhibits an inhibitory effect on a lawn composed of the identical strain. it was shown to occur in seven clinical isolates of enterococci (one e. faecalis and six e. faecium). four involve inhibitory anti-listerial activities consistent with class ii bacteriocins, two of which appear to be up-regulated by extracellular autoinducers. | 2011 | 26781499 |
| physiological and structural differences between enterococcus faecalis jh2-2 and mutant strains resistant to (p)-divercin rv41. | the aim of this study was to show the differences that could exist at the physiological and structural levels between enterococcus faecalis jh2-2 (wild type) and three mutant strains resistant to divercin rv41. these mutant strains were recently isolated and characterized for their intermediate resistance to recombinant dvnrv41; a subclass iia bacteriocin produced by escherichia coli. these mutant strains were named 35a1 (altered in gene coding phosphoesterase activity), 35h1 (altered in gene co ... | 2010 | 26781317 |