Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| recovery of gfp-labeled bacteria for culturing and molecular analysis after cell sorting using a benchtop flow cytometer. | exciting opportunities exist for the application of simple fluorescence-activated cell sorting (facs) to microbiology. the technology is widely available, but critical reports on the efficiency of cell sorting using benchtop instruments are lacking. it is vital that single cell sorting be of the highest purity possible. if purity is compromised detrital material or unwanted cells will be captured along with target cells of interest. here, the isolation of fluorescent bacteria using a benchtop fa ... | 2004 | 15164239 |
| definition of a consensus dna-binding site for pecs, a global regulator of virulence gene expression in erwinia chrysanthemi and identification of new members of the pecs regulon. | in erwinia chrysanthemi, production of pectic enzymes is modulated by a complex network involving several regulators. one of them, pecs, which belongs to the marr family, also controls the synthesis of various other virulence factors, such as cellulases and indigoidine. here, the pecs consensus-binding site is defined by combining a systematic evolution of ligands by an exponential enrichment approach and mutational analyses. the consensus consists of a 23-base pair palindromic-like sequence (c( ... | 2004 | 15140891 |
| partitioning of the variance in the growth parameters of erwinia carotovora on vegetable products. | the objective of this paper was to estimate and partition the variability in the microbial growth model parameters describing the growth of erwinia carotovora on pasteurised and non-pasteurised vegetable juice from laboratory experiments performed under different temperature-varying conditions. we partitioned the model parameter variance and covariance components into effects due to temperature profile and replicate using a maximum likelihood technique. temperature profile and replicate were tre ... | 2004 | 15135958 |
| fast screening method for detection of acyl-hsl-degrading soil isolates. | a reliable method was developed for screening of bacteria isolates capable of degrading acyl-hsls, the signal molecules in quorum-sensing-mediated processes of many proteobacteria. the microtiter assay was based on the use of a gfp-marked escherichia coli strain, which fluoresces upon the presence of acyl-hsls. measurement of gfp fluorescence with a molecular imager fx scanner (fluorometer) detected isolates capable of degrading acyl-hsls. the potential of this method was demonstrated by isolati ... | 2004 | 15134889 |
| genomic diversity of erwinia carotovora subsp. carotovora and its correlation with virulence. | we used genetic and biochemical methods to examine the genomic diversity of the enterobacterial plant pathogen erwinia carotovora subsp. carotovora. the results obtained with each method showed that e. carotovora subsp. carotovora strains isolated from one ecological niche, potato plants, are surprisingly diverse compared to related pathogens. a comparison of 23 partial mdh sequences revealed a maximum pairwise difference of 10.49% and an average pairwise difference of 2.13%, values which are mu ... | 2004 | 15128563 |
| positive control of swarming, rhamnolipid synthesis, and lipase production by the posttranscriptional rsma/rsmz system in pseudomonas aeruginosa pao1. | in pseudomonas aeruginosa, the small rna-binding, regulatory protein rsma is a negative control element in the formation of several extracellular products (e.g., pyocyanin, hydrogen cyanide, pa-il lectin) as well as in the production of n-acylhomoserine lactone quorum-sensing signal molecules. rsma was found to control positively the ability to swarm and to produce extracellular rhamnolipids and lipase, i.e., functions contributing to niche colonization by p. aeruginosa. an rsma null mutant was ... | 2004 | 15126453 |
| stability of short sequence repeats and their application for the characterization of erwinia amylovora strains. | a motif of eight nucleotides (gaattaca) reiterated 3 to 15 times within the psti fragment of the pea29 plasmid was found in erwinia amylovora strains representing a valuable typing method for this pathogen. the stability of short sequence dna repeat (ssr) numbers was investigated to determine the suitability of this marker for strain differentiation. the number of ssr units was found to be stable under laboratory and certain stress conditions. this meets the requirements for a suitable genetic m ... | 2004 | 15109713 |
| production of n-acylhomoserine lactone signal molecules by gram-negative soil-borne and plant-associated bacteria. | quorum-sensing control mediated by n-acylhomoserine lactone (ahl) signal molecules has been established as a key feature in the regulation of various metabolic traits in many bacteria. approximately 300 strains representing 6 genera and 18 species of soil-borne and plant-associated gram-negative bacteria isolated in various regions of the former ussr using two reporter systems were screened for ahl production. the production was observed in 17.5% of the screened bacterial strains. positive respo ... | 2003 | 15058194 |
| asparaginase pharmacodynamics differ by formulation among children with newly diagnosed acute lymphoblastic leukemia. | polyethylene glycol-conjugated (peg) asparaginase is approved for use in patients who develop allergy to other forms of asparaginase, although its ability to deplete asparagine systemically in patients with hypersensitivity has not been well elucidated. in 53 children with newly diagnosed acute lymphoblastic leukemia, we serially assessed asparagine concentrations in cerebrospinal fluid (csf) and plasma as well as serum anti-asparaginase antibodies. all patients received native escherichia coli ... | 2004 | 15057247 |
| thermodependence of growth and enzymatic activities implicated in pathogenicity of two erwinia carotovora subspecies (pectobacterium spp.). | erwinia carotovora subsp. atroseptica and erwinia carotovora subsp. carotovora can cause substantial damage to economically important plant crops and stored products. the occurrence of the disease and the scale of the damage are temperature dependent. disease development consists first of active multiplication of the bacteria in the infection area and then production of numerous extracellular enzymes. we investigated the effects of various temperatures on these two steps. we assayed the specific ... | 2004 | 15052318 |
| molecular differentiation of erwinia amylovora strains from north america and of two asian pear pathogens by analyses of pfge patterns and hrpn genes. | in order to determine a possible genomic divergence of erwinia amylovora'fruit tree' and raspberry strains from north america, several isolates were differentiated by pulsed-field gel electrophoresis (pfge) analysis, the size of short dna sequence repeats (ssrs) and the nucleotide and deduced amino acid sequences of their hrpn genes. by pfge analysis european strains are highly related, whereas strains from north america were diverse and were further distinguished by the ssr numbers from plasmid ... | 2004 | 15049921 |
| one-step purification and kinetic properties of the recombinant l-asparaginase from erwinia carotovora. | ecar-lans, the recombinant l-asparaginase from erwinia carotovora, is a prospective therapeutic enzyme for leukaemia treatment. an efficient and economical scheme was developed for the purification, cloning and expression in eschericha coli of ecar-lans. more than 90% purity, complemented with 72% active enzyme recovery, was achieved with a single chromatographic purification step. the activity of purified l-asparaginase was 630 i.u./mg. the ecar-lans k (m) value was 98x10(-6) m for the main phy ... | 2004 | 15032742 |
| a repeated gga motif is critical for the activity and stability of the riboregulator rsmy of pseudomonas fluorescens. | the riboregulator rsmy of pseudomonas fluorescens strain cha0 is an example of small regulatory rnas belonging to the global rsm/csr regulatory systems controlling diverse cellular processes such as glycogen accumulation, motility, or formation of extracellular products in various bacteria. by binding multiple molecules of the small regulatory protein rsma, rsmy relieves the negative effect of rsma on the translation of several target genes involved in the biocontrol properties of strain cha0. r ... | 2004 | 15031281 |
| management of fire blight: a case study in microbial ecology. | suppression of the blossom-blight phase of fire blight is a key point in the management of this destructive and increasingly important disease of apple and pear. for blossom infection to occur, the causal bacterium, erwinia amylovora, needs to increase its population size through an epiphytic phase that occurs on stigmatic surfaces. knowledge of the ecology of the pathogen on stigmas has been key to the development of predictive models for infection and optimal timing of antibiotic sprays. other ... | 1998 | 15012499 |
| rootstock effects on gene expression patterns in apple tree scions. | like many fruit trees, apple trees (malus pumila) do not reproduce true-to-type from seed. desirable cultivars are clonally propagated by grafting onto rootstocks that can alter the characteristics of the scion. for example, the m.7 emla rootstock is semi-dwarfing and reduces the susceptibility of the scion to erwinia amylovora, the causal agent of fire blight disease. in contrast, the m.9 t337 rootstock is dwarfing and does not alter fire blight susceptibility of the scion. this study represent ... | 2003 | 15010615 |
| systematic analysis, by the yeast two-hybrid, of protein interaction between components of the type ii secretory machinery of erwinia chrysanthemi. | type ii systems allow for the secretion of numerous enzymes and toxins in several gram-negative pathogens. in erwinia chrysanthemi, 14 out proteins are necessary for building the type ii apparatus. we performed a systematic two-hybrid analysis to test interactions between the periplasmic regions of the out proteins. results obtained using this approach suggested that outj (a pseudopilin) was able to interact with (i) outd, the outer membrane secretin, (ii) outi, mainly located in the periplasm, ... | 2004 | 14990257 |
| tetracycline and streptomycin resistance genes, transposons, and plasmids in salmonella enterica isolates from animals in italy. | fifty-eight multidrug-resistant salmonella enterica strains of 20 serotypes, isolated from animal sources in italy, were analyzed for tet(a) and stra-strb, conferring tetracycline and streptomycin resistance, respectively. the stra and strb genes were highly prevalent in salmonella strains of our collection, being detected in 84% of the streptomycin-resistant strains. in many strains, the stra and strb genes were linked to a particular tn5393-derivative transposon characterized by the presence o ... | 2004 | 14982782 |
| the rhas activator controls the erwinia chrysanthemi 3937 genes rhin, rhit and rhie involved in rhamnogalacturonan catabolism. | erwinia chrysanthemi causes soft-rot diseases of various plants by enzymatic degradation of the pectin in plant cell walls. the linear regions of pectin are composed of an acidic sugar, d-galacturonic acid. the ramified regions of pectin also include neutral sugars, and are rich in l-rhamnose residues. e. chrysanthemi is able to degrade these polysaccharides, polygalacturonate and rhamnogalacturonate. in e. chrysanthemi, the production of pectinases acting on linear regions is induced in the pre ... | 2004 | 14982630 |
| bacterial volatiles induce systemic resistance in arabidopsis. | plant growth-promoting rhizobacteria, in association with plant roots, can trigger induced systemic resistance (isr). considering that low-molecular weight volatile hormone analogues such as methyl jasmonate and methyl salicylate can trigger defense responses in plants, we examined whether volatile organic compounds (vocs) associated with rhizobacteria can initiate isr. in arabidopsis seedlings exposed to bacterial volatile blends from bacillus subtilis gb03 and bacillus amyloliquefaciens in937a ... | 2004 | 14976231 |
| evolution of a pathway to novel long-chain carotenoids. | using methods of laboratory evolution to force the c(30) carotenoid synthase crtm to function as a c(40) synthase, followed by further mutagenesis at functionally important amino acid residues, we have discovered that synthase specificity is controlled at the second (rearrangement) step of the two-step reaction. we used this information to engineer crtm variants that can synthesize previously unknown c(45) and c(50) carotenoid backbones (mono- and diisopentenylphytoenes) from the appropriate iso ... | 2004 | 14973014 |
| antifungal activity of beta-asarone from rhizomes of acorus gramineus. | an antifungal substance was isolated from the extract of acorus gramineus using various chromatographic procedures. the antibiotic was identified as beta-asarone, cis-2,4,5-trimethoxy-1-propenylbenzene, on the basis of the high-resolution ei-mass, nmr, and uv spectral data. beta-asarone completely inhibited mycelial growth of some plant pathogenic fungi, cladosporium cucumerinum,colletotrichum orbiculare, magnaporthe grisea, and pythium ultimum, in a range of 0.5-30 microg/ml. the growth of baci ... | 2004 | 14969530 |
| analysis of erwinia chrysanthemi ec16 pele::uida, pell::uida, and hrpn::uida mutants reveals strain-specific atypical regulation of the hrp type iii secretion system. | the plant pathogen erwinia chrysanthemi produces a variety of factors that have been implicated in its ability to cause soft-rot diseases in various hosts. these include hrpn, a harpin secreted by the hrp type iii secretion system; pele, one of several major pectate lyase isozymes secreted by the type ii system; and pell, one of several secondary pels secreted by the type ii system. we investigated these factors in e. chrysanthemi ec16 with respect to the effects of medium composition and growth ... | 2004 | 14964532 |
| importance of opghxcv of xanthomonas campestris pv. vesicatoria in host-parasite interactions. | tn5 insertion mutants of xanthomonas campestris pv. vesicatoria were inoculated into tomato and screened for reduced virulence. one mutant exhibited reduced aggressiveness and attenuated growth in planta. southern blot analyses indicated that the mutant carried a single tn5 insertion not associated with previously cloned pathogenicity-related genes of x. campestris pv. vesicatoria. the wild-type phenotype of this mutant was restored by one recombinant plasmid (popg361) selected from a genomic li ... | 2004 | 14964529 |
| characterization of atypical erwinia carotovora strains causing blackleg of potato in brazil. | to determine the characteristics of bacteria associated with the blackleg disease of potato in brazil and compare them with species and subspecies of pectolytic erwinia. | 2004 | 14962133 |
| isolation and characterization of novel hydrocarbon-degrading euryhaline consortia from crude oil and mangrove sediments. | two novel and versatile bacterial consortia were developed for the biodegradation of hydrocarbons. they were isolated from crude oil from the cormorant field in the north sea (mpd-7) and from sediment associated with mangrove roots (mpd-m). the bacterial consortia were able to degrade both aliphatic and aromatic hydrocarbons in crude oils very effectively in seawater (35 g/l nacl) and synthetic media containing 0 to 100 g/l nacl (1.7 m). salinities over twice that of normal seawater decreased th ... | 2000 | 14961176 |
| fermentation of galacturonic acid and glucose by a strain of erwinia carotovora. | 1952 | 14955520 | |
| pectic enzymes produced by bacterium aroideae. | 1951 | 14833396 | |
| observations on the bacteriophagy of erwinia carotovora. | 1951 | 14824108 | |
| the nutrition of phytopathogenic bacteria. iv. minimal nutritive requirements of the genus erwinia. | 1950 | 14794634 | |
| microbial transformation of pyrethroid insecticides in aqueous and sediment phases. | recent studies showed that synthetic pyrethroids (sps) can move via surface runoff into aquatic systems. fifty-six of sp-degrading bacteria strains were isolated from contaminated sediments, of which six were evaluated for their ability to transform bifenthrin and permethrin in the aqueous phase and bifenthrin in the sediment phase. in the aqueous phase, bifenthrin was rapidly degraded by strains of stenotrophomonas acidaminiphila, and the half-life (t1/2) was reduced from >700 h to 30 to 131 h. ... | 2004 | 14768859 |
| insecticidal bacillus thuringiensis silences erwinia carotovora virulence by a new form of microbial antagonism, signal interference. | it is commonly known that bacteria may produce antibiotics to interfere with the normal biological functions of their competitors in order to gain competitive advantages. here we report that bacillus thuringiensis suppressed the quorum-sensing-dependent virulence of plant pathogen erwinia carotovora through a new form of microbial antagonism, signal interference. e. carotovora produces and responds to acyl-homoserine lactone (ahl) quorum-sensing signals to regulate antibiotic production and expr ... | 2004 | 14766576 |
| norm, an erwinia amylovora multidrug efflux pump involved in in vitro competition with other epiphytic bacteria. | blossoms are important sites of infection for erwinia amylovora, the causal agent of fire blight of rosaceous plants. before entering the tissue, the pathogen colonizes the stigmatic surface and has to compete for space and nutrient resources within the epiphytic community. several epiphytes are capable of synthesizing antibiotics with which they antagonize phytopathogenic bacteria. here, we report that a multidrug efflux transporter, designated norm, of e. amylovora confers tolerance to the tox ... | 2004 | 14766544 |
| integration of environmental and host-derived signals with quorum sensing during plant-microbe interactions. | many plant-associated microbes use secreted autoinducer molecules, including n-acylhomoserine lactones (ahls), to regulate diverse behaviours in association with their population density (quorum sensing). often, these responses are affected by environmental conditions, including the presence of other ahl-producing bacterial species. in addition, plant-derived metabolites, including products that arise as a direct result of the bacterial infection, may profoundly influence ahl-regulated behaviour ... | 2004 | 14764105 |
| early responses of resistant and susceptible potato roots during invasion by the potato cyst nematode globodera rostochiensis. | signals from roots of resistant (cv. maris piper) and susceptible (cv. désirée) potato cultivars during invasion by second stage juveniles (j2s) of the potato cyst nematode, globodera rostochiensis, were investigated. novel experimental chambers enabled the recording of electrophysiological responses from roots during nematode invasion. the root cell membrane potentials were maintained throughout the 3 d required to assess invasion and feeding site development. the steady-state resting membrane ... | 2004 | 14754919 |
| erwinia papayae sp. nov., a pathogen of papaya (carica papaya). | bacterial canker of papaya (carica papaya) emerged during the 1980s in different islands of the caribbean. nineteen strains of gram-negative, rod-shaped, non-spore-forming bacteria isolated from papaya were compared to 38 reference and type strains of phytopathogenic enterobacteriaceae and related bacteria. phylogenetic analysis of 16s rrna gene sequences showed that the papaya strains belonged to the genus erwinia. the dna g+c content of strain cfbp 5189t, 52.5 mol%, is in the range of the genu ... | 2004 | 14742466 |
| cyp175a1 from thermus thermophilus hb27, the first beta-carotene hydroxylase of the p450 superfamily. | the biological function of thermostable p450 monooxygenase cyp175a1 from thermus thermophilus hb27 was studied by functional complementation in escherichia coli. the gene product of cyp175a1 added hydroxyl groups to both beta rings of beta-carotene to form zeaxanthin (beta,beta-carotene-3,3'-diol) in e. coli, which produces beta-carotene due to the erwinia uredovora carotenoid biosynthesis genes. in addition, spectroscopic methods revealed that e. coli carrying cyp175a1 and the cdna of the haema ... | 2004 | 14727092 |
| genetic characterization of erwinia amylovora strains by amplified fragment length polymorphism. | erwinia amylovora is one of the most important pathogens of pear and apple and is subject to strict quarantine regulations worldwide, although its patterns of dispersal are largely unknown. previous attempts to fingerprint e. amylovora strains by molecular techniques have detected very little polymorphism because of the high genetic homogeneity of this bacterium. our aim was to establish and test a typing method to quantify genetic diversity among strains of this plant pathogen. | 2004 | 14723691 |
| the phytoalexin-inducible multidrug efflux pump acrab contributes to virulence in the fire blight pathogen, erwinia amylovora. | the enterobacterium erwinia amylovora causes fire blight on members of the family rosaceae, with economic importance on apple and pear. during pathogenesis, the bacterium is exposed to a variety of plant-borne antimicrobial compounds. in plants of rosaceae, many constitutively synthesized isoflavonoids affecting microorganisms were identified. bacterial multidrug efflux transporters which mediate resistance toward structurally unrelated compounds might confer tolerance to these phytoalexins. to ... | 2004 | 14714867 |
| an evolutionary hot spot: the pngr234b replicon of rhizobium sp. strain ngr234. | rhizobium sp. strain ngr234 has an exceptionally broad host range and is able to nodulate more than 112 genera of legumes. since the overall organization of the ngr234 genome is strikingly similar to that of the narrow-host-range symbiont rhizobium meliloti strain 1021 (also known as sinorhizobium meliloti), the obvious question is why are the spectra of hosts so different? study of the early symbiotic genes of both bacteria (carried by the syma plasmids) did not provide obvious answers. yet, bo ... | 2004 | 14702322 |
| plastic phenotypic resistance to predation by bdellovibrio and like organisms in bacterial prey. | predation at the lowest trophic level, i.e. between bacteria, is poorly understood, hindering efforts to assess its impact on the structure of bacterial communities. the interaction of bdellovibrio and bacteriovorax (bdellovibrio and like organisms, blos), a group of obligate, ubiquitous predatory bacteria, with their gram-negative bacterial prey results in the multiplication of the predator and in the lysis, but not in the eradication, of the prey. we show that the residual, surviving populatio ... | 2004 | 14686937 |
| crystallization of the pectate lyase peli from erwinia chrysanthemi and sad phasing of a gold derivative. | the pectate lyase peli is involved in the degradation of plant tissues by the phytopathogenic bacterium erwinia chrysanthemi. it has been crystallized from a solution containing peg 550 in the space group p2(1), with unit-cell parameters a = 61.6, b = 70.7, c = 73.4 a, beta = 112.8 degrees. crystals diffract to 1.45 a using synchrotron radiation. sad phases have been computed from a gold-derivative crystal at the wavelength of maximum absorption (l(iii) edge). | 2004 | 14684926 |
| growth phase-dependent regulation and stringent control of fis are conserved processes in enteric bacteria and involve a single promoter (fis p) in escherichia coli. | the intracellular concentration of the escherichia coli factor for inversion stimulation (fis), a global regulator of transcription and a facilitator of certain site-specific dna recombination events, varies substantially in response to changes in the nutritional environment and growth phase. under conditions of nutritional upshift, fis is transiently expressed at very high levels, whereas under induced starvation conditions, fis is repressed by stringent control. we show that both of these regu ... | 2004 | 14679232 |
| cloning of the pela gene from bacillus licheniformis 14a and biochemical characterization of recombinant, thermostable, high-alkaline pectate lyase. | the pectate lyase gene pela from alkaliphilic bacillus licheniformis strain 14a was cloned and sequenced. the nucleotide sequence corresponded to an open reading frame of 1,026 bp that codes for a 39 amino acid signal peptide and a mature protein with a molecular mass of 33,451 da. the mature pela showed significant homology to other pectate lyases belonging to polysaccharide lyase family 1, such as enzymes from different bacillus spp. and erwinia chrysanthemi. the pela gene was expressed in esc ... | 2004 | 14673544 |
| the crystal structure of pectate lyase pel9a from erwinia chrysanthemi. | the "family 9 polysaccharide lyase" pectate lyase l (pel9a) from erwinia chrysanthemi comprises a 10-coil parallel beta-helix domain with distinct structural features including an asparagine ladder and aromatic stack at novel positions within the superhelical structure. pel9a has a single high affinity calcium-binding site strikingly similar to the "primary" calcium-binding site described previously for the family pel1a pectate lyases, and there is strong evidence for a common second calcium ion ... | 2004 | 14670977 |
| hydrodynamic properties of oxidized extracellular polysaccharides from erwinia chrysanthemi spp. | the molecular weights of the native polysaccharides of erwinia chrysanthemi strains range from 1.8 to 7.1 x 10(6) and their hydrodynamic properties are those of polydisperse, polyanionic biopolymers with pseudoplastic, non-thixotropic flow characteristics in aqueous solutions. the effect on the hydrodynamic properties of the polysaccharides by adding carboxyl groups to increase the charge density is studied, with particular reference to their molecular weight (mw), viscosity and conformation. in ... | 2003 | 14670735 |
| oxidative burst elicited by bacillus mycoides isolate bac j, a biological control agent, occurs independently of hypersensitive cell death in sugar beet. | response of sugar beet cultivars c40 and ush11 to syringe infiltration of live and dead bacillus mycoides isolate bac j, a biological control agent, and virulent and avirulent isolates of erwinia carotovora pv. betavasculorum was measured by monitoring systemic acquired resistance control of cercospora beticola, specific activity of chitinase and beta-glucanase, the oxidative burst, and hypersensitive cell death at the infiltration site. priming sugar beet with b. mycoides bac j (1 x 10(8) cells ... | 2003 | 14651348 |
| gaca, the response regulator of a two-component system, acts as a master regulator in pseudomonas syringae pv. tomato dc3000 by controlling regulatory rna, transcriptional activators, and alternate sigma factors. | concerted investigations of factors affecting host-pathogen interactions are now possible with the model plant arabidopsis thaliana and its model pathogen pseudomonas syringae pv. tomato dc3000, as their whole genome sequences have become available. as a prelude to analysis of the regulatory genes and their targets, we have focused on gaca, the response regulator of a two-component system. the dc3000 gene was cloned by testing for the reversal of phenotypes of an erwinia gaca- mutant. a gaca- mu ... | 2003 | 14651344 |
| coordinate expression of multiple bacterial carotenoid genes in canola leading to altered carotenoid production. | carotenoids have drawn much attention recently because of their potentially positive benefits to human health as well as their utility in both food and animal feed. previous work in canola (brassica napus) seed over-expressing the bacterial phytoene synthase gene (crtb) demonstrated a change in carotenoid content, such that the total levels of carotenoids, including phytoene and downstream metabolites like beta-carotene, were elevated 50-fold, with the ratio of beta- to alpha-carotene being 2:1. ... | 2003 | 14642353 |
| tomato pectin methylesterase: modeling, fluorescence, and inhibitor interaction studies-comparison with the bacterial (erwinia chrysanthemi) enzyme. | the molecular model of lycopersicon esculentum (tomato) pectin methylesterase (pme) was built by using the x-ray crystal structure of pme from the phytopathogenic bacterium erwinia chrysanthemi as a template. the overall structure and the position of catalytically important residues (asp132, asp 153, and arg 221, located at the bottom of the active site cleft) are conserved. instead, loop regions forming the walls of the catalytic site are much shorter and form a less deep cleft, as already reve ... | 2003 | 14635125 |
| rsmy, a small regulatory rna, is required in concert with rsmz for gaca-dependent expression of biocontrol traits in pseudomonas fluorescens cha0. | in the plant-beneficial soil bacterium and biocontrol model organism pseudomonas fluorescens cha0, the gacs/gaca two-component system upregulates the production of biocontrol factors, i.e. antifungal secondary metabolites and extracellular enzymes, under conditions of slow, non-exponential growth. when activated, the gacs/gaca system promotes the transcription of a small regulatory rna (rsmz), which sequesters the small rna-binding protein rsma, a translational regulator of genes involved in bio ... | 2003 | 14622422 |
| the quorum sensing negative regulators esar and expr(ecc), homologues within the luxr family, retain the ability to function as activators of transcription. | most luxr homologues function as activators of transcription during the process of quorum sensing, but a few, including esar and expr(ecc), negatively impact gene expression. the luxr-activated luxi promoter and luxr binding site, the lux box, were used in artificial contexts to assess the potential for transcriptional activation and dna binding by esar and expr(ecc). although the acyl-homoserine lactone responsiveness of both proteins is the opposite of that shown by most luxr family members, e ... | 2003 | 14617666 |
| the pmra-pmrb two-component system responding to acidic ph and iron controls virulence in the plant pathogen erwinia carotovora ssp. carotovora. | efficient response to environmental cues is crucial to successful infection by plant-pathogenic bacteria such as erwinia carotovora ssp. carotovora. the expression of the main virulence genes of this pathogen, encoding extracellular enzymes that degrade the plant-cell wall, is subject to complex regulatory machinery where two-component systems play an important role. in this paper, we describe for the first time the involvement of the pmra-pmrb two-component system in regulation of virulence in ... | 2003 | 14617142 |
| influence of peanut oil on microbial degradation of polycyclic aromatic hydrocarbons. | peanut oil amendment (0.1%-0.2% (v/v)) increased the biodegradation of various polycyclic aromatic hydrocarbons (pahs) by 15%-80% with a mixed bacterial culture and a pure culture of comamonas testosteroni in aqueous media and in pah-contaminated weathered soil slurry systems. the stimulatory effect on biodegradation was more pronounced with the high molecular weight pahs (e.g., >3 rings). the presence of peanut oil also accelerated the biodegradation of pahs sorbed onto activated carbon, indica ... | 2003 | 14608386 |
| development of biobased products. | research conducted over the past seven years by the biotechnology byproducts consortium (bbc) addresses its mission to investigate the opportunities to add value to agricultural products, byproducts and coproducts and to manage the wastewater arising from agribusinesses in an environmentally favorable way. since a wide variety of research approaches have been taken, the results are collected in five topic groups: (1) bioremediation that includes anaerobic fermentations of wastes to produce metha ... | 2004 | 14585619 |
| purification and characterization of a lactonase from erwinia cypripedii 314b that hydrolyzes ( s)-5-oxo-2-tetrahydrofurancarboxylic acid. | a bacterium, strain 314b, able to assimilate ( s)-5-oxo-2-tetrahydrofurancarboxylic acid was isolated from soil and identified as erwinia cypripedii. a lactonase hydrolyzing ( s)-5-oxo-2-tetrahydrofurancarboxylic acid to l-alpha-hydroxyglutaric acid was purified 63-fold with 2% recovery from crude extracts of this bacterium to homogeneity as judged by sds-page. the molecular masses estimated by sds-page and gel filtration were 41 kda and 79 kda, respectively. the maximum activity was observed at ... | 2003 | 14576976 |
| characterization of the hrp pathogenicity cluster of erwinia carotovora subsp. carotovora: high basal level expression in a mutant is associated with reduced virulence. | extracellularly targeted proteins are crucial for virulence of gram-negative phytopathogenic bacteria. erwinia carotovora subsp. carotovora employs the so-called type ii (gsp) pathway to secrete a number of pectinases and cellulases, which cause the typical tissue maceration symptoms of soft-rot disease. the type iii (hrp) pathway is the major virulence determinant in the genera pseudomonas, ralstonia and xanthomonas, and in non-macerating species of erwinia. the hrp cluster was recently partial ... | 2003 | 14576934 |
| genus- and isolate-specific real-time pcr quantification of erwinia on leaf surfaces of english oaks (quercus robur l.). | in order to quantify pathogenic epiphytic bacteria on leaf surfaces of the important european forest tree quercus robur without time-intensive cultivation and separation of microorganisms, methods were developed to selectively quantify dna copy numbers of the genus erwinia in dna isolated from the leaf surface. by using the combination of the two different real-time pcr techniques sybr-green and taqman, methods were developed not only to allow quantification of the total dna copy number of erwin ... | 2003 | 14570272 |
| genetic designs for product formation in recombinant microbes. | several new bacterial host-vector systems for klebsiella, erwinia, xanthomonas, nocardia, and streptomyces have been developed. with these host-vector systems, a strain of klebsiella, which overproduces the extracellular starch-debranching enzyme, pullulanase, has been developed. the gene for cholesterol oxidase was cloned and used to develop a strain of streptomyces lividans that extracellularly produces the enzyme, cholesterol oxidase, which is utilized to process cholesterol and diagnosticall ... | 1990 | 14545902 |
| approach to maceration mechanism in enzymatic pulping of bast fibers by alkalophilic pectinolytic enzymes produced by erwinia species. | tissue maceration was generally elucidated by the action of endo-polygalacturonase and endo-pectate or -pectin lyase (endo-pal or -pnl). in a process of screening of erwinia and pseudomonas strains for enzymatic pulping of pectocellulosic bast fibers, it was found that their pal productivity was not completely related with defibration activity, i.e., the fact that an e.chrysanthemi strain showed high pal productivity but possessed rather low defibration activity. moreover, defibration activity w ... | 1988 | 14543440 |
| tyrosine phenol-lyase: the role of the coenzyme-binding residue ser-254 in catalysis. | 2003 | 14531074 | |
| frameshift mutation events in beta-glucosidases. | compensated frameshift mutation is a modification of the reading frame of a gene that takes place by way of various molecular events. it appears to be a widespread event that is only observed when homologous amino acid and nucleodotide sequences are compared. to identify these mutation events, the sequence analysis rationale was based on the search for short regions that would have much lower degrees of conservation in protein, but not in dna, in well-conserved beta-glucosidase families. we have ... | 2003 | 14527732 |
| identification of klebsiella oxytoca using a specific pcr assay targeting the polygalacturonase pehx gene. | bacteria of the genus klebsiella are important opportunistic pathogens responsible for nosocomial infections that are increasingly resistant to antimicrobial agents. distinctive identification of the species k. oxytoca, k. pneumoniae, k. planticola, k. ornithinolytica and k. terrigena is difficult based on phenotypic tests and misidentifications are frequent in routine clinical microbiology. we developed a specific method to discriminate k. oxytoca from the other species of the genus klebsiella, ... | 2003 | 14527660 |
| properties of a novel extracellular cell-free ice nuclei from ice-nucleating pseudomonas antarctica in-74. | some ice-nucleating bacterial strains, including pantoea ananatis (erwinia uredovora), pseudomonas fluorescens, and pseudomonas syringae isolates, were examined for the ability to shed ice nuclei into the growth medium. a novel ice-nucleating bacterium, pseudomonas antarctica in-74, was isolated from ross island, antarctica. cell-free ice nuclei from p. antarctica in-74 were different from the conventional cell-free ice nuclei and showed a unique characterization. cell-free ice nuclei were purif ... | 2003 | 14519981 |
| the influence of antibiotic production and pre-emptive colonization on the population dynamics of pantoea agglomerans (erwinia herbicola) eh1087 and erwinia amylovora in planta. | stigma colonization by erwinia amylovora is the crucial first step in the development of most fire blight infections in apple and pear trees. suppression at this point of the disease process by antagonists of e. amylovora, such as pantoea agglomerans (erwinia herbicola) strain eh1087, is a rational approach to control fire blight. we tested the hypothesis that the ability of e. amylovora to compete with eh1087 for colonization of a stigma is reduced by the potential for eh1087 to produce the phe ... | 2003 | 14510856 |
| structure of the o-polysaccharide of erwinia carotovora ssp. carotovora gspb 436. | the o-polysaccharide of a phytopathogenic bacterium, erwinia carotovora ssp. carotovora gspb 436, was studied by sugar and methylation analysis, along with 1h and 13c nmr spectroscopy. the following structure of the branched tetrasaccharide repeating unit of the o-polysaccharide was established: [carbohydrate structure in text] the o-polysaccharide contains a minor proportion of 4-o-methylrhamnose, which is suggested to terminate the polymer main chain. | 2003 | 14499581 |
| catabolism of galacturonic and glucuronic acids by erwinia carotovora. | 1959 | 14409051 | |
| effects of plant-growth-regulating substances on control of erwinia amylovora by streptomycin and terramycin. | 1955 | 14385830 | |
| action of cycloserine on a species of erwinia with reference to cell division. | 1965 | 14346120 | |
| [nucleotide composition of desoxyribonucleic and ribonucleic acids of pathogens of toxic bacteriosis of watermelons]. | 1964 | 14336859 | |
| [pectolytic activity of erwinia carotovora]. | 1964 | 14335561 | |
| isolation and characterization of bacteriophages active against stalked bacteria. | 1965 | 14328417 | |
| bacteria responsible for mucilage-layer decomposition in kona coffee cherries. | the predominant microbial flora present during decomposition of the mucilage layer of kona coffee cherries were gram-negative bacteria which fermented lactose rapidly. cultures isolated from coffee cherries under-going fermentation included species of erwinia, paracolobactrum, and escherichia. unblemished cherry surfaces and coffee plantation soil also had a microflora containing a high proportion of bacteria belonging to these three genera. of 168 isolates tested, the 44 strains capable of demu ... | 1965 | 14325879 |
| comparative evaluation of five selective and differential media for the detection and enumeration of coagulase-positive staphylococci in foods. | five selective media for the detection and enumeration of coagulase-positive staphylococci were evaluated for their efficiency in the recovery of 17 strains of coagulase-positive staphylococci from foods. they were staphylococcus medium 110 (sm-110), tellurite-glycine-agar (tga), egg-tellurite-glycine-pyruvate-agar (etgpa), tellurite-egg-agar (tea), and tellurite-polymyxin-egg yolk-agar (tpey). statistical analysis by the rank correlation method of the efficiency with which these media recovered ... | 1965 | 14325870 |
| erwinia-like microorganisms isolated from animal and human hosts. | erwinia-like microorganisms were isolated from the vital organs of more than 40% of a large deer population examined as well as from 13% of a small random sampling. over a brief period of observation, a similar isolate was recovered from throat cultures of two children and one adolescent. there is no obvious explanation of the presence of the plant pathogen or its significance under these circumstances. | 1965 | 14325868 |
| antifungal activity of a series of substituted ((alpha-nitroalkyl)benzylthio)alkylamines. | 1965 | 14323149 | |
| postirradiation growth, division, and recovery in bacteria. | 1965 | 14276328 | |
| in vitro and in vivo interactions between components of mixed bacterial cultures isolated from apple buds. | 1965 | 14272786 | |
| microbial interactions: population and sporulation studies of bacillus sphaericus grown in association with erwinia atroseptica. | 1964 | 14160080 | |
| pigmentation and taxonomy of the genus xanthomonas. | starr, mortimer p. (university of california, davis), and william l. stephens. pigmentation and taxonomy of the genus xanthomonas. j. bacteriol. 87:293-302. 1964.-the colonies formed by phytopathogenic bacteria of the genus xanthomonas are typically yellow in color. this chromogenesis stems from the presence in all yellow xanthomonads of a particular carotenoid pigment. this unique "xanthomonas-carotenoid," which has not been found in any yellow nonxanthomonad, is characterized herein to the ext ... | 1964 | 14151047 |
| 6-aminopenicillanic acid: inhibition of destruction of cephalosporin c by bacteria. | 6-aminopenicillanic acid, from which all the true penicillins are derived, inhibits the destruction of cephalosporin c by certain microorganisms. this inhibition is probably specific for "cephalosporinase." the enzymatic deacetylation of cephalosporin c is not affected by the acid. | 1964 | 14107444 |
| effect of kinetin on bacteria. | 1963 | 14087910 | |
| inhibition in synthesis of beta-alanine by d-serine. | 1963 | 14078942 | |
| [biochemical micromethod for group identification of intestinal bacteria]. | 1963 | 14049322 | |
| [research on the nutrition of erwinia carotovora]. | 1963 | 14048732 | |
| [on the development of resistance in erwinia carotovora (jones) holland and xanthomonas campestris (pammel) dowson to thiosulfate ethers]. | 1963 | 14048461 | |
| [apropos of the use of sodium gluatmate in lyophilization of bacteria]. | 1963 | 14020341 | |
| cell division in a species of erwinia. vi. growth of cells from the division end. | 1962 | 13950914 | |
| cell division in a species of erwinia. v. effect of metabolic inhibitors on terminal division and composition of a "division" medium. | grula, e. a. (oklahoma state university, stillwater) and mary m. grula. cell division in a species of eriwinia. v. effect of metabolic inhibitors on terminal division and composition of a "division" medium. j. bacteriol. 84:492-499. 1962.-terminal division in erwinia spp. involves a triggering action and subsequent septum synthesis. it is a metabolic process requiring organic nitrogen and carbon and energy. the process, when triggered by pantoic acid or pantoyl lactone, is inhibited strongly by ... | 1962 | 13950913 |
| a selective-diagnostic medium for the isolation of pectinolytic organisms in the enterobacteriaceae. | 1962 | 13917121 | |
| eliminative split of pectic substances by phytopathogenic soft-rot bacteria. | culture filtrates of erwinia carotovora and bacillus polymyxa split pectic substances to yield a product that absorbs at 230 mmicro, and which reacts with thiobarbituric acid to form a substance that absorbs at 547 mmicro. this product is believed to be a c-4,5-unsaturated oli-gouronide. the preferred substrate is polygalacturonic acid rather than pectin; the enzyme, provisionally named polygalacturonic-trans-eliminase, is activated by calcium. | 1962 | 13916373 |
| [the reciprocal antagonistic properties among the erwinia. discussion of the taxonomic position of this genus]. | 1961 | 13904218 | |
| reversal of mitomycin c-induced growth and division inhibition in a species of erwinia. | 1962 | 13902421 | |
| cell division in a species of erwinia iv. metabolic blocks in panothenate biosynthesis and their relationship to inhibition of cell division. | grula, mary m. (oklahoma state university, stillwater) and e. a. grula. cell division in a species of erwinia. iv. metabolic blocks in pantothenate biosynthesis and their relationship to inhibition of cell division. j. bacteriol. 83:989-997. 1962.-four compounds that inhibit cell division in an erwinia sp., d-serine, d-histidine, d-phenylalanine, and penicillin, decrease the intracellular pantothenate content of erwinia at culture ages of 10 and 16 hr. in the case of penicillin, it appears to be ... | 1962 | 13902420 |
| cell division in a species of erwinia iii. reversal of inhibition of cell division caused by d-amino acids, penicillin, and ultraviolet light. | grula, e. a. (oklahoma state university, stillwater) and mary m. grula. cell division in a species of erwinia. iii. reversal of inhibition of cell division caused by d-amino acids, penicillin, and ultraviolet light. j. bacteriol. 83:981-988. 1962.-inhibition of cell division in an erwinia sp. occurs in the presence of any of six d-amino acids, penicillin, or ultraviolet light. cell-division inhibition caused by d-amino acids is ph-dependent; however, elongation caused by penicillin occurs over a ... | 1962 | 13902419 |
| intermediary metabolism of carbohydrate by erwinia amylovora. | 1960 | 13835844 | |
| anaerobic dissimilation of glucose by erwinia amylovora. | 1959 | 13835843 | |
| [determination of total ascorbic acid by the use of a suspension of erwinia solanisapra. 2. specificity of the reduction of dehydroascorbic acid by these bacteria. modifications contributed to the determination of technic]. | 1960 | 13827575 | |
| [study of a strain of erwinia (erwinia salmonis nv. sp.) isolated from a common trout (salmo fario l.)]. | 1959 | 13804497 | |
| [research on a germ of the family of pseudomonadaceae (order of pseudomonadeae) erwinia nimipressularis isolated from a fresh sausage]. | 1960 | 13804496 | |
| an association between capsulation and phage sensitivity in erwinia amylovora. | 1960 | 13800707 |