Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| comparison of bacterial lipopolysaccharides by high-performance liquid chromatography. | a comparison of lipid-free polysaccharides from gram-negative bacteria was rapidly accomplished by using high-performance liquid chromatography of underivatized hydrolysates. examination of a number of such products revealed that, contrary to earlier reports, xanthomonas campestris lipopolysaccharide contained heptose, together with rhamnose and galactose, but not mannose. the polymers from the methanotrophs "methylomonas albus" and "methylosinus trichosporium" contained heptose and glucose, and ... | 1986 | 16347189 |
| bacterial growth rates and competition affect nodulation and root colonization by rhizobium meliloti. | the addition of streptomycin to nonsterile soil suppressed the numbers of bacterial cells in the rhizosphere of alfalfa (medicago sativa l.) for several days, resulted in the enhanced growth of a streptomycin-resistant strain of rhizobium meliloti, and increased the numbers of nodules on the alfalfa roots. a bacterial mixture inoculated into sterile soil inhibited the colonization of alfalfa roots by r. meliloti, caused a diminution in the number of nodules, and reduced plant growth. enterobacte ... | 1986 | 16347173 |
| cloning, expression, and purification of glutamine synthetase from clostridium acetobutylicum. | a glutamine synthetase (gs) gene, glna, from the gram-positive obligate anaerobe clostridium acetobutylicum was cloned on recombinant plasmid phz200 and enabled escherichia coli glna deletion mutants to utilize (nh(4))(2)so(4) as a sole source of nitrogen. the cloned c. acetobutylicum gene was expressed from a regulatory region contained within the cloned dna fragment. glna expression was subject to nitrogen regulation in e. coli. this cloned glna dna did not enable an e. coli glna ntrb ntrc del ... | 1986 | 16347143 |
| microbial penetration through nutrient-saturated berea sandstone. | penetration times and penetration rates for a motile bacillus strain growing in nutrient-saturated berea sandstone cores were determined. the rate of penetration was essentially independent of permeabilities above 100 mdarcys and rapidly declined for permeabilities below 100 mdarcys. it was found that these penetration rates could be grouped into two statistically distinct classes consisting of rates for permeabilities above 100 mdarcys and rates for those below 100 mdarcys. instantaneous penetr ... | 1985 | 16346858 |
| nitrogen-fixing bacteria from warty lenticellate bark of a mangrove tree, bruguiera gymnorrhiza (l.) lamk. | detached warty lenticellate bark of a mangrove tree species, bruguiera gymnorrhiza (l.) lamk. from iriomote island, okinawa, a subtropical region of japan, showed development of acetylene reduction activity when incubated in a mineral nutrient solution lacking nitrogen under an atmosphere consisting of 5% o(2), 90% n(2), and 5% c(2)h(2). the bacteria responsible for nitrogen fixation were isolated from the bark, and their capacity for acetylene reduction and the incorporation of n(2) into the ba ... | 1984 | 16346458 |
| adaptation to cadmium by klebsiella aerogenes growing in continuous culture proceeds mainly via formation of cadmium sulfide. | the adaptation of klebsiella aerogenes to high levels of cadmium was studied in continuous culture under conditions of glucose limitation. when up to 6 x 10 m cadmium was added to a culture in steady state, growth ceased instantaneously but resumed within 5 h (dilution rate, 0.1 h). when again in steady state, these adapted cells exhibited a far greater tolerance to cadmium than did unadapted cells (not previously exposed to cadmium) when tested on solid media containing different concentrations ... | 1982 | 16346119 |
| comparison of bacterial extracellular polymer extraction methods. | five different bacterial extracellular polymer extraction methods and a combination of two of these methods were compared on cultures of activated sludge, synthetic activated sludge, and klebsiella aerogenes. high-speed centrifugation was the most effective extraction method for the k. aerogenes culture, based on the comparatively small amount of cell disruption and the relatively high extracellular polymer yield. steaming treatment was the most effective extraction method for the activated slud ... | 1980 | 16345600 |
| acetylene reduction (nitrogen fixation) by enterobacteriaceae isolated from paper mill process waters. | using selective media containing galactitol, over 130 enterobacteriaceae have been isolated from paper mill process waters collected from different localities. these bacteria were extensively characterized and tested for acetylene-reducing (nitrogen-fixing) activity under anaerobic conditions. high activity was found in representatives of klebsiella pneumoniae, enterobacter aerogenes, enterobacter cloacae, erwinia herbicola, citrobacter freundii, citrobacter intermedius, and escherichia coli. un ... | 1976 | 16345168 |
| hydrogen production from glucose by anaerobes. | various anaerobes were cultivated in media containing glucose. when 100 ml of thioglycollate medium containing 2.0% (w/v) glucose was used, clostridium butyricum atcc 859, nbrc 3315, and nbrc 13949 evolved 227-243 ml of biogas containing about 180 ml of hydrogen in 1 day. although some strains had some resistance against oxygen, c. butyricum atcc 859 and 860 did not have it. c. butyricum nbrc 3315 and enterobacter aerogenes nbrc 13534 produced hydrogen in the presence of glucose or pyruvic acid, ... | 2005 | 16321068 |
| differential activity of a lectin from solieria filiformis against human pathogenic bacteria. | a lectin isolated from the red alga solieria filiformis was evaluated for its effect on the growth of 8 gram-negative and 3 gram-positive bacteria cultivated in liquid medium (three independent experiments/bacterium). the lectin (500 microg/ml) stimulated the growth of the gram-positive species bacillus cereus and inhibited the growth of the gram-negative species serratia marcescens, salmonella typhi, klebsiella pneumoniae, enterobacter aerogenes, proteus sp, and pseudomonas aeruginosa at 1000 m ... | 2005 | 16302091 |
| minimum leak size determination, under laboratory and commercial conditions, for bacterial entry into polymeric trays used for shelf-stable food packaging. | this study sought to determine the minimum leak size for entry of enterobacter aerogenes under laboratory conditions, and normal flora under commercial conditions, into tryptic soy broth with yeast extract (tsbye), homestyle chicken, and beef enchilada packaged in 355-ml polyethylene terephthalate/ethylene vinyl alcohol/polypropylene trays. channel leaks (diameters of 50 to 200 microm) were made across the sealing area of the trays. pinholes (diameters of 5 to 50 microm) were made by imbedding l ... | 2005 | 16300076 |
| [profiles of the utilization of 20 amino acids as the only source of nitrogen and carbon in bacteria of the genera klebsiella, enterobacter, serratia, escherichia]. | the profiles of the utilization of 20 protein amino acids in 118 klebsiella pneumoniae sub- sp. pneumoniae, k. oxytoca, k. planticola, k. mobilis, enterobacter cloacae, serratia marscescens, s. liquefaciens, escherichia coli strains isolated from clinical material were studied. the utilization of amino acids was determined on minimal saline agar containing amino acid as the only source of nitrogen and carbon; the results were evaluated after 72-hour incubation at 37 degrees c. 17 profiles of ami ... | 2005 | 16279526 |
| [purification and characterization of a uridine phosphorylase from enterobacter aerogenes eam-z1]. | a uridine phosphorylase(upase) was isolation from enterobacter aerogenes eam-z1 and purified by means of ammonium sulfate precipitation, deae-cellulose, phenyl-sepharose, deae-sepharose, fplc ion exchange, and sephacryl s-200 column chromatography. the purified upase showed homogeneity on the native polacrylamide gel electrophoresis. the upase is a trimer of 43 kd subunits. fifteen residues from the amino terminal end of upase were identified as mrmvdliatkrdgge. the isoelectric point was ph 4.46 ... | 2003 | 16279202 |
| identification of three urease accessory proteins that are required for urease activation in arabidopsis. | urease is a nickel-containing urea hydrolase involved in nitrogen recycling from ureide, purine, and arginine catabolism in plants. the process of urease activation by incorporation of nickel into the active site is a prime example of chaperone-mediated metal transfer to an enzyme. four urease accessory proteins are required for activation in klebsiella aerogenes. in plants urease accessory proteins have so far been only partially defined. using reverse genetic tools we identified four genes tha ... | 2005 | 16244137 |
| hydrogen and ethanol production from glycerol-containing wastes discharged after biodiesel manufacturing process. | h2 and ethanol production from glycerol-containing wastes discharged after a manufacturing process for biodiesel fuel (biodiesel wastes) using enterobacter aerogenes hu-101 was evaluated. the biodiesel wastes should be diluted with a synthetic medium to increase the rate of glycerol utilization and the addition of yeast extract and tryptone to the synthetic medium accelerated the production of h2 and ethanol. the yields of h2 and ethanol decreased with an increase in the concentrations of biodie ... | 2005 | 16243274 |
| antibiotic resistance in outpatient urinary isolates: final results from the north american urinary tract infection collaborative alliance (nautica). | the goal of the north american urinary tract infection collaborative alliance (nautica) study was to determine antibiotic susceptibility to commonly used agents for urinary tract infections against outpatient urinary isolates obtained in various geographic regions in the usa and canada. forty-one medical centres (30 from the usa and 11 from canada) participated, with each centre submitting up to 50 consecutive outpatient midstream urine isolates. isolates were identified to species level by the ... | 2005 | 16243229 |
| high-yield production of hydrogen by enterobacter aerogenes mutants with decreased alpha-acetolactate synthase activity. | to enhance hydrogen (h2) production from glucose by enterobacter aerogenes hu-101, two mutants, strains vp-1 and vp-2, with decreased alpha-acetolactate synthase activity, were isolated using the voges-proskauer (vp) test. in ph-uncontrolled batch culture, both mutants showed a lower 2,3-butanediol yield for the glucose consumed than that shown by the wild-type strain, although glucose remained in the medium after 12 h of culture. in the same cultures, compared to the h2 yield of 0.80 mol/mol-gl ... | 2004 | 16233620 |
| acid phosphatase/phosphotransferases from enteric bacteria. | we have investigated the enzymatic phosphorylation of nucleosides and found that morganella morganii phoc acid phosphatase exhibits regioselective pyrophosphate (pp(i))-nucleoside phosphotransferase activity. in this study, we isolated genes encoding an acid phosphatase with regioselective phosphotransferase activity (ap/ptase) from providencia stuartii, enterobacter aerogenes, escherichia blattae and klebsiella planticola, and compared the primary structures and enzymatic characteristics of the ... | 2001 | 16233057 |
| microbial hydrogen production from sweet potato starch residue. | clostridium butyricum could produce hydrogen from a sweet potato starch residue upon supplementation of nitrogen sources. a repeated batch culture using a mixed culture of c. butyricum and enterobacter aerogenes produced hydrogen with a yield of 2.4 mol h2/mol glucose under a controlled culture ph of 5.25 in a medium consisting of the sweet potato starch residue and 0.1% polypepton without addition of any reducing agents. rhodobacter sp. m-19 produced hydrogen from the supernatant of the culture ... | 2001 | 16232947 |
| clinical and molecular analysis of extended-spectrum {beta}-lactamase-producing enterobacteria in the community setting. | during a previous survey, five extended-spectrum beta-lactamase (esbl)-producing enterobacteria (esble) (two enterobacter aerogenes isolates expressing tem-24 b, two escherichia coli isolates expressing tem-21 or tem-24 b, and one klebsiella pneumoniae isolate expressing shv-4/tem-15) responsible for urinary tract infections (utis) were found among 1,584 strains collected from community patients. the aim of the present study was to elucidate the route of emergence of these typically nosocomial o ... | 2005 | 16207960 |
| metabolic activation of bladder procarcinogens, 2-aminofluorene, 4-aminobiphenyl, and benzidine by pseudomonas aeruginosa and other human endogenous bacteria. | pseudomonas aeruginosa, an opportunistic pathogen of the human urinary tract, and other selected human endogenous bacteria were investigated for metabolic activation of the bladder procarcinogens, 2-aminofluorene (2-af), 4-aminobiphenyl (4-ab), and benzidine (bz). the cell-free extracts of pseudomonas aeruginosa, escherichia coli, enterobacter aerogenes, proteus mirabilis, proteus vulgaris, staphylococcus epidermidis, staphylococcus saprophyticus, klebsiella pneumoniae, and intestinal anaerobes, ... | 2006 | 16203120 |
| occurrence and regulation of the ferric citrate transport system in escherichia coli b, klebsiella pneumoniae, enterobacter aerogenes, and photorhabdus luminescens. | in escherichia coli k-12, transcription of the ferric citrate transport genes fecabcde is initiated by binding of diferric dicitrate to the outer membrane protein feca which elicits a signaling cascade from the cell surface to the cytoplasm. the feci sigma factor is only active in the presence of fecr, which transfers the signal across the cytoplasmic membrane. in other bacteria, fecira homologues control iron transport gene transcription by siderophores other than citrate. however, in most case ... | 2005 | 16193283 |
| bacterial species specificity in prou osmoinducibility and nptii and lacz expression. | reporter gene-based transcriptional fusions are increasingly being used to address questions in microbial ecology, with constitutively expressed fusions enabling microbe tracking and inducible fusions reporting the presence of environmental signals. to more readily apply this technology to a variety of bacterial species, we examined species specificity in the expression of three promoters of interest. a comparison of two potentially constitutive promoters, each fused to the reporter gene gfp, sh ... | 2004 | 16179797 |
| inorganic polyphosphate in the social life of myxococcus xanthus: motility, development, and predation. | inorganic polyphosphate (poly p), a polymer of tens or hundreds of phosphate residues linked by high-energy, atp-like bonds, is found in all organisms and performs a wide variety of functions. myxococcus xanthus, a social bacterium that feeds on other bacteria and forms fruiting bodies and spores, depends on poly p for motility, development, and nutritional predation. two poly p metabolizing enzymes were studied in m. xanthus: poly p kinase 1, which synthesizes poly p reversibly from atp, and po ... | 2005 | 16174737 |
| production, acceptability and microbiological evaluation of weaning food formulations. | weaning food formulations were produced from pearl millet cultivar (sosat c-88), cowpea and groundnut in the ratio of 70:20:10 and 5 per cent malt from six pearl millet cultivars (sosat c-88, zango, ex-borno, icmv is 94206, gwagwa, gb 8735) and one sorghum cultivar (icsv iii) were added to produce seven formulations plus the one without malt. microbiological quality of eight weaning food formulations was evaluated. twenty weaning mothers were used to determine the acceptability of gruels from th ... | 2006 | 16169859 |
| investigations on anti-aspergillus properties of bacterial products. | to investigate the anti-aspergillus properties of bacterial products. | 2005 | 16162136 |
| a solvent tolerant isolate of enterobacter aerogenes. | a solvent tolerant strain of enterobacter aerogenes was isolated from soil by cyclohexane enrichment. presence of cyclohexane (20%) in culture media prolonged the lag phase and caused reduction in biomass. transmission electron micrographs showed convoluted cell membrane and accumulation of solvent in case of the cells grown in cyclohexane. the enterobacter isolate was able to grow in the range of organic solvents having log p above 3.2 and also in presence of mercury, thus showing potential for ... | 2006 | 16154507 |
| type 1 sphingosine 1-phosphate g protein-coupled receptor signaling of lymphocyte functions requires sulfation of its extracellular amino-terminal tyrosines. | the type 1 sphingosine 1-phosphate (s1p) g protein-coupled receptor (s1p1) transduces signals from s1p that mediate thymocyte emigration, t cell transmigration of lymph nodes, and t cell chemotaxis in tissues. alterations in expression of functional s1p1 receptors by lymphocytes are the major mechanisms controlling their responses to s1p and were thought to be solely a consequence of the balance between surface down-regulation and insertion. however, results now show that lack of sulfation of ty ... | 2005 | 16148028 |
| the susceptibility of certain bacterial strains to imidazotriazine derivatives. | the synthesized imidazo[2,1-c]triazines were tested for their antimicrobial activity. microbiological screening tests conducted in relation to 54 strains of gram-positive and 52 strains of gram-negative bacteria, 6 strains of yeast-like fungi and 3 strains of moulds showed significant activity of compound 19 against 52 gram-negative bacterial strains. this compound was active against 16 strains of escherichia coli, 12 strains of pseudomonas, 10 strains of proteus spp., 8 strains of klebsiella pn ... | 2004 | 16146057 |
| a simplified method for assay of hydrogenase activities of h2 evolution and uptake in enterobacter aerogenes. | assay of hydrogenase activity pertaining to h2 production needs anaerobic conditions. to establish a simplified method for assay of hydrogenase activities by using intact cells of enterobater aerogenes, different chemicals capable of enhancing the cell-wall permeability to electron mediators were examined. as a result, triton x-100 and ctab were found to be appropriate for h2 uptake and evolution activities of the intact cells, respectively. this method enabled h2 uptake and evolution activities ... | 2005 | 16132849 |
| a study on inhibitory effects of siğla tree (liquidambar orientalis mill. var. orientalis) storax against several bacteria. | in this study, siğla (liquidambar orientalis mill.) storax (styrax) was investigated for antibacterial activity against aeromonas hydrophila, bacillus amyloliquefaciens, b. brevis, b. cereus, b. megaterium, b. subtilis, corynebacterium xerosis, enterobacter aerogenes, enterococcus faecalis, escherichia coli, e. coli o157:h7, klebsiella pneumoniae, listeria monocytogenes, micrococcus luteus, mycobacterium smegmatis, proteus vulgaris, pseudomonas aeruginosa, p. fluorescens, staphylococcus aureus a ... | 2005 | 16114094 |
| transmembrane transcriptional control (surface signalling) of the escherichia coli fec type. | the ferric citrate transport system of escherichia coli is the first example of a transcription initiation mechanism that starts at the cell surface. the inducer, ferric citrate, binds to an outer membrane transport protein, and without further transport elicits a signal that is transmitted across the outer membrane, the periplasm, and the cytoplasmic membrane into the cytoplasm. signal transfer across the three subcellular compartments is mediated by the outer membrane transport protein that in ... | 2004 | 16102597 |
| rapid detection of a gfp-marked enterobacter aerogenes under anaerobic conditions by aerobic fluorescence recovery. | a gfp- and kanamycin-resistance gene-containing plasmid pucgk was successfully constructed and transformed into enterobacter aerogenes to develop a rapid gfp-based method for quantifying the bacterial concentration under anaerobic conditions for production of biohydrogen. since the use of gfp as a molecular reporter is restricted by its requirement for oxygen in the development of the fluorophore, fluorescence detection for the fluorescent e. aerogenes grown anaerobically for hydrogen production ... | 2005 | 16006057 |
| aetiology of catheter-associated bacteriuria in lagos university teaching hospital. | a prospective study to determine the causative agents of catheter-related bacteriuria was carried out on 99 patients with median age of 55 years, on admission in the oncology, paediatric surgery and obstetric and gynaecology wards of the lagos university teaching hospital. there was significant bacteriuria in seventy-one (72%) of the 99 urine samples. all catheter urine samples were contaminated by day 5 gram-negative bacilli were predominant (89.3%) of which pseudomonas aeruginosa (26.2%) was t ... | 2005 | 15997255 |
| spread of novel expanded-spectrum beta-lactamases in enterobacteriaceae in a university hospital in the paris area, france. | in 2002, 28 non-duplicate enterobacterial isolates producing extended-spectrum beta-lactamases (esbls) were collected from infected patients at the bicêtre hospital in paris, france. escherichia coli was the predominant esbl-positive enterobacterial species, comprising ten (36%) of the isolates. ctx-m enzymes (ctx-m-3, ctx-m-10, ctx-m-14 and ctx-m-15) were produced by 11 (39%) of the isolates (six e. coli, two enterobacter cloacae, one enterobacter aerogenes, one proteus mirabilis and one citrob ... | 2005 | 15966981 |
| multidrug resistance in gram-negative bacteria that produce extended-spectrum beta-lactamases (esbls). | in 1983, just two years after the introduction of the oxymino-beta-lactams to the market , the first extended-spectrum beta-lactamases were isolated in germany from klebsiella pneumoniae strains. since then several outbreaks have been reported in many european countries and the usa, and nowadays in several places worldwide the problem seems to reach endemic dimensions, with rates exceeding 50% in some countries, such as portugal and turkey. on the other hand not only k. pneumoniae but also esche ... | 2005 | 15953019 |
| enterobacter meningitis: organism susceptibilities, antimicrobial therapy and related outcomes. | meningitis due to enterobacter species is an uncommon infection in adults; however, when present, treatment is frequently complicated by resistance of many enterobacter isolates to third-generation cephalosporins and poor central nervous system penetration of other antibiotics. the aim of this study was to retrospectively review cases of meningitis caused by enterobacter species at our institution, to better characterize patient factors, pathogen characteristics, and treatment options for this i ... | 2005 | 15936376 |
| microorganisms and parasites in chronic infective diarrhea. | to reveal the pattern of microorganisms in chronic infective diarrhea cases. | 2004 | 15931720 |
| antidiarrhoeal activity of the methanol extract of the barks of xylocarpus moluccensis in castor oil- and magnesium sulphate-induced diarrhoea models in mice. | the methanol (meoh) extract of the barks of xylocarpus moluccensis, and different fractions of this extract were studied for antidiarrhoeal activity using castor oil- and magnesium sulphate-induced diarrhoea models in mice. at the doses of 250 and 500 mg/kg, the meoh extract showed significant antidiarrhoeal activity in both models. the etoac fraction (eaf) and the residual meoh fraction (rmf) exhibited similar activity using a dose of 250 mg/kg in both models. no antidiarrhoeal activity was obs ... | 2005 | 15905054 |
| the eefabc multidrug efflux pump operon is repressed by h-ns in enterobacter aerogenes. | the enterobacter aerogenes eefabc locus, which encodes a tripartite efflux pump, was cloned by complementation of an escherichia coli tolc mutant. e. aerogenes deltaacra expressing eefabc became less susceptible to a wide range of antibiotics. data from eef::lacz fusions showed that eefabc was not transcribed in the various laboratory conditions tested. however, increased transcription from peef was observed in an e. coli hns mutant. in addition, eefa was detected in e. aerogenes expressing a do ... | 2005 | 15901719 |
| chemical compositions and antimicrobial activities of four different anatolian propolis samples. | propolis means a gum that is gathered by bees from various plants. it is known for its biological properties, having antibacterial, antifungal and healing properties. the aims of this study were to evaluate the antimicrobial activity of four different anatolian propolis samples on different groups of microorganisms including some oral pathogens and comparison between their chemical compositions. ethanol extracts of propolis (eep) were prepared from four different anatolian propolis samples and e ... | 2005 | 15881836 |
| purification and properties of the klebsiella aerogenes uree metal-binding domain, a functional metallochaperone of urease. | klebsiella aerogenes uree, a metallochaperone that delivers nickel ions during urease activation, consists of distinct "peptide-binding" and "metal-binding" domains and a his-rich c terminus. deletion analyses revealed that the metal-binding domain alone is sufficient to facilitate urease activation. this domain was purified and shown to exhibit metal-binding properties similar to those of uree lacking only the his-rich tail. | 2005 | 15866948 |
| increased expression of a bacterial phosphotriesterase in escherichia coli through directed evolution. | we devised a growth-based strategy for screening phosphotriesterase mutant libraries for variants with enhanced activity towards organophosphates that generate dimethyl phosphate when hydrolysed. phosphotriesterase mutants were screened for activity by growing transformed escherichia coli on agar plates containing methyl paraoxon as a sole phosphorus source. e. coli is capable of growth under these conditions when coexpressing the phosphotriesterase from agrobacterium radiobacter p230 (opda) and ... | 2005 | 15866732 |
| effects of formate on fermentative hydrogen production by enterobacter aerogenes. | this paper describes the effects of formate on fermentative hydrogen production by enterobacter aerogenes by way of batch culture. when 20 mm formate was added to ph 6.3 and ph 5.8 e. aerogenes glucose cultures (formate culture) at the beginning of cultivation, hydrogen evolution through both glucose consumption and decomposition of the extrinsic formate occurred together, while hydrogen evolution occurred only through glucose consumption in the control cultures. the hydrogen evolution rates in ... | 2005 | 15830121 |
| cefamandole, a cephalosporin antibiotic with an unusually wide spectrum of activity. | the in vitro activity of cefamandole, 7-d-mandelamido-3-(1 methyl-1h-tetrazol-5 yethiomethyl)-3-cephem-4 carboxylic acid, was investigated. the majority of streptococci and pneumococci were inhibited by 0.1 mug/ml. activity against staphylococcus aureus was below that of cephalothin, but most strains were inhibited by 0.4 mug/ml. enterococci were not inhibited by less than 25 mug/ml. cefamandole was very active against most members of the enterobacteriaceae, with 70% of escherichia coli, 86% of ... | 1974 | 15828189 |
| meningitis due to enterobacter aerogenes subsequent to resection of an acoustic neuroma and abdominal fat graft to the mastoid. | meningitis is an uncommon complication of neurosurgical procedures, with an incidence of 1.1% to 2.5%. although unusual, the frequency of nosocomial gram-negative meningitis appears to be increasing. gram-negative meningitis has been documented following disruption of the dura-arachnoid barrier secondary to trauma or surgery. the association of gram-negative bacillary meningitis with neurosurgical procedures was first reported in the 1940's. wolff et al. described the association between enterob ... | 2004 | 15798816 |
| characterization of a novel nadp(+)-dependent d-arabitol dehydrogenase from the plant pathogen uromyces fabae. | we have identified and characterized a novel nadp(+)-dependent d-arabitol dehydrogenase and the corresponding gene from the rust fungus uromyces fabae, a biotrophic plant pathogen on broad bean (vicia faba). the new enzyme was termed ard1p (d-arabitol dehydrogenase 1). it recognizes d-arabitol and mannitol as substrates in the forward reaction, and d-xylulose, d-ribulose and d-fructose as substrates in the reverse reaction. co-factor specificity was restricted to nadp(h). kinetic data for the ma ... | 2005 | 15796718 |
| successive emergence of enterobacter aerogenes strains resistant to imipenem and colistin in a patient. | enterobacter aerogenes is an agent of hospital-acquired infection that exhibits a remarkable resistance to beta-lactam antibiotics during therapy. five successive isolates of e. aerogenes infecting a patient and exhibiting a multiresistance phenotype to beta-lactam antibiotics and fluoroquinolones were investigated. among these clinical strains, four presented resistant phenotypes during successive imipenem and colistin treatments. the involved resistance mechanisms exhibited by the successive i ... | 2005 | 15793111 |
| (1)h nmr spectroscopy in the diagnosis of pseudomonas aeruginosa-induced urinary tract infection. | the utility of (1)h nmr spectroscopy is suggested and demonstrated for the diagnosis of pseudomonas aeruginosa in urinary tract infection (uti). the specific property of p. aeruginosa of metabolizing nicotinic acid to 6-hydroxynicotinic acid (6-ohna) is exploited. the quantity of 6-ohna produced correlates well with the viable bacterial count. other common bacteria causing uti such as escherichia coli, klebsiella pneumonia, enterobacter aerogenes, acinetobacter baumanii, proteus mirabilis, citro ... | 2005 | 15759292 |
| chloramphenicol and expression of multidrug efflux pump in enterobacter aerogenes. | chloramphenicol has been reported to act as an inducer of the multidrug resistance in escherichia coli. a resistant variant able to grow on plates containing 64 microg/ml chloramphenicol was obtained from the enterobacter aerogenes atcc 13048-type strain. chloramphenicol resistance was due to an active efflux of this antibiotic and it was associated with resistance to fluoroquinolones and tetracycline, but not to aminoglycoside or beta-lactam antibiotics. mdr in the chloramphenicol-resistant var ... | 2005 | 15707992 |
| inorganic polyphosphate in dictyostelium discoideum: influence on development, sporulation, and predation. | dictyostelium discoideum, a social slime mold that forms fruiting bodies with spores, depends on inorganic polyphosphate (poly p) for its cycles of development and for nutritional predation on bacteria. the synthesis of poly p, a polymer of tens or hundreds of phosphate residues linked by high energy, atp-like bonds, is catalyzed in most bacteria by poly p kinase (ppk1). the eukaryote d. discoideum possesses a homolog of ppk1. we report here that mutants of d. discoideum ppk1 (ddppk1) have reduc ... | 2005 | 15701689 |
| mutational analysis of beta-glucoside utilization in klebsiella aerogenes: evidence for the presence of multiple genetic systems. | 2004 | 15689631 | |
| vitreoscilla hemoglobin renders enterobacter aerogenes highly susceptible to heavy metals. | when expressed in heterologous microorganisms vitreoscilla hemoglobin (vhb) acts as oxygen storage and causes a higher oxygen uptake. in this study, the effect of this protein on growth, sensitivity and antioxidant properties of enterobacter aerogenes exposed to metal stress was investigated. the strain expressing vhb was more sensitive to mercury and cadmium as the minimal inhibitory concentration (mic) for these metals was up to 2-fold lower in this strain than the host and the recombinant str ... | 2004 | 15689114 |
| detection and characterization of extended-spectrum beta-lactamases among bloodstream isolates of enterobacter spp. in hong kong, 2000-2002. | a total of 139 consecutive and non-duplicate bloodstream isolates of enterobacter spp. collected from inpatients in hong kong during 2000-2002 were studied for production of extended-spectrum beta-lactamases (esbls). | 2005 | 15681579 |
| [prosthetic valve endocarditis due to enterobacter aerogenes]. | 2005 | 15639331 | |
| antibacterial constituents from the berries of piper nigrum. | piper nigrum finds an extensive application in antibacterial preparations belonging to ayurvedic system of medicine. a bioguided extraction and fractionation of the petroleum ether extract of the berries of p. nigrum afforded 2e, 4e, 8z-n-isobutyleicosatrienamide (1), pellitorine (2), trachyone (3), pergumidiene (4) and isopiperolein b (5). pergumidiene and trachyone are isolated for the first time from p. nigrum. all the isolated compounds were active against bacillus subtilis, bacillus sphaeri ... | 2004 | 15636188 |
| [outbreak of enterobacter aerogenes in paediatric unit]. | within the framework of breast milk control the hygiene laboratory of brest hospital isolates, on 3 january 1996 a strain of enterobacter aerogenes secretory of cephalosporinase in the breast milk of a mother whose child was hospitalized in neonatalogy. on 15 april 1996 a new strain of e. aerogenes is isolated from another mother's breast milk. until 18 august 1997, 21 samples of breast milk were tested positive to this bacteria. during the same period, e. aerogenes was isolated in 26 children u ... | 2004 | 15619887 |
| extended-spectrum beta-lactamase (esbl) producing enterobacter aerogenes phenotypically misidentified as klebsiella pneumoniae or k. terrigena. | enterobacter aerogenes and klebsiella pneumoniae are common isolates in clinical microbiology and important as producers of extended spectrum beta-lactamases (esbl). the discrimination between both species, which is routinely based on biochemical characteristics, is generally accepted to be straightforward. here we report that genotypically unrelated strains of e. aerogenes can be misidentified as k. pneumoniae by routine laboratories using standard biochemical identification and using identific ... | 2004 | 15619329 |
| antibacterial activity and specificity of the six human {alpha}-defensins. | we developed a kinetic, 96-well turbidimetric procedure that is capable of testing the antimicrobial properties of six human alpha-defensins concurrently on a single microplate. the defensins were prepared by solid-phase peptide synthesis and tested against gram-positive bacteria (staphylococcus aureus and bacillus cereus) and gram-negative bacteria (enterobacter aerogenes and escherichia coli). analysis of the growth curves provided virtual lethal doses (vlds) equivalent to conventional 50% let ... | 2005 | 15616305 |
| [investigation of pathogenic bacteria for pulmonary infections and their drug resistance in neurological intensive care unit]. | to investigate the pathogenic bacterial spectrum responsible for pulmonary infections and their drug resistance in patients admitted to neurological care unit. | 2004 | 15604082 |
| histidine decarboxylase in enterobacteriaceae revisited. | with a modification of taylor's decarboxylation broth, histidine decarboxylase was detected in enterobacter aerogenes, morganella morganii, raoultella ornithinolytica, and some strains of citrobacter youngae and raoultella planticola. this method provides a useful confirmatory test for identification of e. aerogenes strains. | 2004 | 15583342 |
| tem-121, a novel complex mutant of tem-type beta-lactamase from enterobacter aerogenes. | enterobacter aerogenes clinical isolate lor was resistant to penicillins and ceftazidime but susceptible to cefuroxime, cephalothin, cefoxitin, cefotaxime, ceftriaxone, and cefepime. pcr and cloning experiments from this strain identified a novel tem-type beta-lactamase (tem-121) differing by five amino acid substitutions from beta-lactamase tem-2 (glu104lys, arg164ser, ala237thr, glu240lys, and arg244ser) and by only one amino acid change from the extended-spectrum beta-lactamase (esbl) tem-24 ... | 2004 | 15561821 |
| influence of positive end-expiratory pressure (peep) on histopathological and bacteriological aspects of pneumonia during low tidal volume mechanical ventilation. | ventilatory strategies combining low tidal volume (v(t)) with positive end-expiratory pressure (peep) are considered to be lung protective. the influence of the peep level was investigated on bacteriology and histology in a model of ventilator-associated pneumonia. | 2004 | 15536527 |
| intestinal microflora in 45 crows in ueno zoo and the in vitro susceptibilities of 29 escherichia coli isolates to 14 antimicrobial agents. | microorganisms from 45 jungle crows (corvus macrorhynchos) captured from july to december 2002 at ueno zoo, tokyo were identified as escherichia coli, proteus mirabilis, klebsiella pneumoniae, klebsiella oxytoca, enterobacter aerogenes, enterobacter cloacae, enterobacter agglomerans, pseudomonas maltophila, staphylococcus spp., micrococcus spp., and streptococcus spp. e. coli showed the highest rate of isolation (21.6%). in an in vitro susceptibility test for 29 isolates of e. coli to 14 antimic ... | 2004 | 15528866 |
| [nosocomial pneumonia due to resistant enterobacter aerogenes]. | 2004 | 15509050 | |
| synthesis and antimicrobial activity of some netropsin analogues. | nine novel lexitropsins were synthesized by linking two netropsin-like moieties through three different dicarboxylic acids; 9,10-dihydro-2,7-phenanthrenedicarboxylic acid; [(3-[[(carboxymethyl)amino]carbonyl]benzoyl)amino]acetic acid and indole-2,5-dicarboxylic acid. the netropsin residues were modified by the use of n-isopentylpyrrole, 5-methylthiophene or 5-isopropylthiazole heterocyclic building blocks in place of the usual n-methylpyrrole. the compounds were tested against five gram-positive ... | 2004 | 15505717 |
| [rapid diagnosis of common pathogenic bacteria infection in newborn infants by 16srdna oligonucleotide array]. | the rapid identification of pathogenic bacteria is important for earlier effective patient management and antimicrobial therapy, especially for the infant patient, whose immunological system is not fully developed. however conventional microbiogical techniques of bacterial identification, culture and isolation of pathogenic bacteria, identification by biochemistry and serological assay, are time-consuming and require intensive labor. on the basis of special gene sequence, pcr provides simple and ... | 2004 | 15482667 |
| evaluation of the microscan esbl plus confirmation panel for detection of extended-spectrum beta-lactamases in clinical isolates of oxyimino-cephalosporin-resistant gram-negative bacteria. | we aimed to assess the performance of the microscan esbl plus confirmation panel using a series of 87 oxyimino-cephalosporin-resistant gram-negative bacilli of various species. | 2004 | 15471997 |
| correlation of extended spectrum beta lactamases production with cephalosporin resistance in gram negative bacilli. | beta lactamase production is an important mechanism of developing resistance to beta lactam group of antibiotics. cephlosporins with extended spectrum of activity and stability were introduced to overcome this resistance, but soon production of extended spectrum beta lactamases (esbls), which are inducible in nature was reported. in this study klebsiella aerogenes--166, escherichia coli--120, citrobacter spps--116, pseudomonas spps--50, proteus spps--32 and s. typhi--16, strains were subjected t ... | 2004 | 15471144 |
| detection of low levels of listeria monocytogenes cells by using a fiber-optic immunosensor. | biosensor technology has a great potential to meet the need for sensitive and nearly real-time microbial detection from foods. an antibody-based fiber-optic biosensor to detect low levels of listeria monocytogenes cells following an enrichment step was developed. the principle of the sensor is a sandwich immunoassay where a rabbit polyclonal antibody was first immobilized on polystyrene fiber waveguides through a biotin-streptavidin reaction to capture listeria cells on the fiber. capture of cel ... | 2004 | 15466560 |
| effect of smoking on vitamin a, vitamin e, and other trace elements in patients with cardiovascular disease in bangladesh: a cross-sectional study. | data regarding the impact of cigarette smoking on trace elements are scarce and inconsistent. in this study, we evaluated the effect of smoking on serum concentrations of trace elements among adult males with heart disease. | 2004 | 15462677 |
| [occurrence of escherichia coli, aerobacter aerogenes and intermediate forms in the blood]. | 1950 | 15443730 | |
| [formation of acetoin and 2,3-butylglycol with the culture of aerobacter aerogenes]. | 1950 | 15438747 | |
| acetic acid oxidation by escherichia coli and aerobacter aerogenes. | 1950 | 15436423 | |
| the early-lag phase of aerobacter aerogenes. | 1949 | 15394454 | |
| false positive diagnosis of gonorrheal cervicitis and urethritis produced by aerobacter aerogenes; case report. | 1949 | 15393638 | |
| dissemination of transferable ampc-type beta-lactamase (cmy-10) in a korean hospital. | to determine dissemination and genotype of ampc beta-lactamases and an extended-spectrum beta-lactamase among clinical isolates of enterobacteriaceae, we performed antibiotic susceptibility testing, pi determination, induction test, plasmid profiles, transconjugation test, enterobacterial repetitive consensus (eric)-pcr, and dna sequencing. among the 51 clinical isolates collected from a university hospital in korea, six isolates were resistant to cephamycins. all six isolates produced a plasmid ... | 2004 | 15383166 |
| co-infection of the macronucleus of paramecium caudatum by free-living bacteria together with the infectious holospora obtusa. | infection experiments were performed incubating paramecium caudatum with non-infectious free-living bacteria or weakly infectious intracellular bacteria together with the infectious holospora obtusa. two of four non-infectious free-living bacteria (enterobacter aerogenes and klebsiella pneumoniae) were found to get into the nuclei when added to paramecium together with h. obtusa. the endonuclear bacterium nonospora macronucleata that is weakly infectious by itself increases its infectivity when ... | 2004 | 15352323 |
| the acrab-tolc pump is involved in macrolide resistance but not in telithromycin efflux in enterobacter aerogenes and escherichia coli. | the role of the acrab-tolc pump in macrolide and ketolide susceptibility in escherichia coli and enterobacter aerogenes was studied. efflux pump inhibitor restored erythromycin, clarithromycin, and telithromycin susceptibilities to multidrug-resistant isolates. no modification of telithromycin accumulation was detected in e. aerogenes acrab or tolc derivatives compared to that in the parental strain. two independent efflux pumps, inhibited by phenylalanine arginine beta-naphthylamide, expel macr ... | 2004 | 15328143 |
| enhanced production of acetoin and butanediol in recombinant enterobacter aerogenes carrying vitreoscilla hemoglobin gene. | microbial production of butanediol and acetoin has received increasing interest because of their diverse potential practical uses. although both products are fermentative in nature, their optimal production requires a low level of oxygen. in this study, the use of a recombinant oxygen uptake system on production of these metabolites was investigated. enterobacter aerogenes was transformed with a puc8-based plasmid carrying the gene (vgb) encoding vitreoscilla (bacterial)hemoglobin (vhb). the pre ... | 2004 | 15309606 |
| molecular epidemiology of enterobacteriaceae isolates producing extended-spectrum beta-lactamases in a french hospital. | in 2002, 80 isolates of enterobacteriaceae producing extended-spectrum beta-lactamases (esbls) were collected from infected patients in our hospital. enterobacter aerogenes was the most common bacterium isolated from all specimens (36.5%). the esbls were predominantly (90%) tem derivatives (tem-24, tem-3). pulsed-field gel electrophoresis highlighted that e. aerogenes, klebsiella pneumoniae, and citrobacter koseri had a clonal propagation. | 2004 | 15297534 |
| recommended test panel for differentiation of klebsiella species on the basis of a trilateral interlaboratory evaluation of 18 biochemical tests. | klebsiella pneumoniae and klebsiella oxytoca are the two most frequently encountered klebsiella species giving rise to infections in humans, but other klebsiella species can also be found in clinical specimens: klebsiella ozaenae, klebsiella rhinoscleromatis, klebsiella terrigena, klebsiella planticola, klebsiella ornithinolytica, and enterobacter aerogenes (klebsiella mobilis). however, many of these species are indistinguishable by the conventional methods employed routinely in the clinical mi ... | 2004 | 15297514 |
| spermidine/spermine-n1-acetyltransferase-2 (ssat2) acetylates thialysine and is not involved in polyamine metabolism. | spermidine/spermine-n1-acetyltransferase (ssat1) is a short-lived polyamine catabolic enzyme inducible by polyamines and polyamine analogues. induction of ssat1 plays an important role in polyamine homoeostasis, since the n1-acetylated polyamines can be excreted or oxidized by acetylpolyamine oxidase. we have purified a recombinant human acetyltransferase (ssat2) that shares 45% identity and 61% homology with human ssat1, but is only distally related to other known members of the gnat (gcn5-rela ... | 2004 | 15283699 |
| cloning and expression of the vitreoscilla hemoglobin gene in enterobacter aerogenes: effect on cell growth and oxygen uptake. | the hemoglobins found in unicellular organisms show a greater chemical reactivity, protect cells against oxidative stress and hence have been implicated in a wider variety of potential functions than those traditionally associated with animal and plant hemoglobins. there are well-documented studies showing that bacteria expressing vitreoscilla hemoglobin (vhb), the first prokaryotic hemoglobin characterized, have better growth and oxygen uptake rates than vhb counterparts. | 2004 | 15283330 |
| frequency and diversity of class a extended-spectrum beta-lactamases in hospitals of the auvergne, france: a 2 year prospective study. | to evaluate the frequency and diversity of extended-spectrum beta-lactamases (esbls) produced by enterobacteriaceae and pseudomonas aeruginosa in one french region. | 2004 | 15282240 |
| emerging from obscurity: biological, clinical, and diagnostic aspects of dientamoeba fragilis. | ever since its first description in 1918, dientamoeba fragilis has struggled to gain recognition as a significant pathogen. there is little justification for this neglect, however, since there exists a growing body of case reports from numerous countries around the world that have linked this protozoal parasite to clinical manifestations such as diarrhea, abdominal pain, flatulence, and anorexia. a number of studies have even incriminated d. fragilis as a cause of irritable bowel syndrome, aller ... | 2004 | 15258093 |
| evaluation of the hyplex bloodscreen multiplex pcr-enzyme-linked immunosorbent assay system for direct identification of gram-positive cocci and gram-negative bacilli from positive blood cultures. | we evaluated the hyplex bloodscreen pcr-enzyme-linked immunosorbent assay (elisa) system (bag, lich, germany), a new diagnostic test for the direct identification of gram-negative bacilli and gram-positive cocci from positive blood cultures, with 482 positive bactec 9240 blood culture bottles. the test involves amplification of the bacterial dna by multiplex pcr and subsequent hybridization of the pcr product to specific oligonucleotide probes in an elisa-based format. the available probes allow ... | 2004 | 15243074 |
| enterobacter aerogenes ompx, a cation-selective channel mar- and osmo-regulated. | the ompx gene of enterobacter aerogenes was cloned. its overexpression induced a decrease in the major porin omp36 production and consequently a beta-lactam resistance was noted. purified outer membrane protein x (ompx) was reconstituted into artificial membranes and formed ion channels with a conductance of 20 ps in 1 m nacl and a cationic selectivity. both mara expression and high osmolarity induced a noticeable increase of the ompx synthesis in the e. aerogenes atcc 13048 strain. in addition, ... | 2004 | 15225603 |
| evaluation of the uro-quick, a new rapid automated system, for the detection of well-characterized antibiotic-resistant bacteria. | the uro-quick system has been employed to detect antibiotic resistance in genotypically and/or phenotypically well-characterized bacterial species including those that might not be easily identified by routine procedure. in order to achieve full agreement between the antibiotic susceptibility results obtained by the reference method (nccls) and the uro-quick system, the optimal experimental conditions (inoculum size, time of incubation and antibiotic concentration) for each strain to be used by ... | 2004 | 15216942 |
| rama is an alternate activator of the multidrug resistance cascade in enterobacter aerogenes. | multidrug resistance (mdr) in enterobacter aerogenes can be mediated by induction of mara, which is triggered by certain antibiotics and phenolic compounds. in this study, we identified the gene encoding rama, a 113-amino-acid regulatory protein belonging to the arac-xyls transcriptional activator family, in the enterobacter aerogenes atcc 13048 type strain and in a clinical multiresistant isolate. overexpression of rama induced an mdr phenotype in drug-susceptible escherichia coli jm109 and e. ... | 2004 | 15215103 |
| the outer membrane protein omp35 affects the reduction of fe(iii), nitrate, and fumarate by shewanella oneidensis mr-1. | shewanella oneidensis mr-1 uses several electron acceptors to support anaerobic respiration including insoluble species such as iron(iii) and manganese(iv) oxides, and soluble species such as nitrate, fumarate, dimethylsulfoxide and many others. mr-1 has complex branched electron transport chains that include components in the cytoplasmic membrane, periplasm, and outer membrane (om). previous studies have implicated a role for anaerobically upregulated om electron transport components in the use ... | 2004 | 15212692 |
| antiseptic impregnated endotracheal tubes for the prevention of bacterial colonization. | the effect of endotracheal tubes (etts) impregnated with chlorhexidine (chx) and silver carbonate (antiseptic etts) against staphylococcus aureus, methicillin-resistant s. aureus (mrsa), pseudomonas aeruginosa, acinetobacter baumannii, and enterobacter aerogenes [organisms associated with ventilator-associated pneumonia (vap)], was evaluated in a laboratory airway model. antiseptic etts and control etts (unimpregnated) were inserted in culture tubes half-filled with agar media (airway model) pre ... | 2004 | 15183249 |
| comparative in vitro and in vivo study of nine alcohol-based handrubs. | hygienic hand disinfection using alcohol-based handrubs (ahrs) is an alternative method to conventional handwashing in hospital wards. because a documented choice of such products would consider data from in-care evaluation, we designed a comparative study of 9 ahrs both in vitro and in vivo in actual care conditions. | 2004 | 15175613 |
| use of pharmacodynamic end points in the evaluation of gatifloxacin for the treatment of acute maxillary sinusitis. | the relationship between drug exposure and the time course of antimicrobial effect at the primary infection site for acute maxillary sinusitis has not previously been explored. this single-center, open-label study quantified the time course of sinus sterilization, described gatifloxacin exposure at the infection site, and posed the hypothesis that the use of continuous and quantitative time-related end points may allow for better characterization of drug effect with fewer patients than tradition ... | 2004 | 15156435 |
| omp35, a new enterobacter aerogenes porin involved in selective susceptibility to cephalosporins. | in enterobacter aerogenes, beta-lactam resistance often involves a decrease in outer membrane permeability induced by modifications of porin synthesis. in atcc 15038 strain, we observed a different pattern of porin production associated with a variable antibiotic susceptibility. we purified omp35, which is expressed under conditions of low osmolality and analyzed its pore-forming properties in artificial membranes. this porin was found to be an ompf-like protein with high conductance values. it ... | 2004 | 15155215 |
| evaluation of a new cefepime-clavulanate esbl etest to detect extended-spectrum beta-lactamases in an enterobacteriaceae strain collection. | in this study, we evaluated the performance of a new esbl etest configuration based on clavulanate synergy with cefepime compared with cefotaxime-clavulanate and ceftazidime-clavulanate esbl etest strips for the detection of extended-spectrum beta-lactamases (esbl) in an enterobacteriaceae strain collection, with special focus on enterobacter spp. | 2004 | 15150168 |
| the twilight of heliozoa and rise of rhizaria, an emerging supergroup of amoeboid eukaryotes. | recent molecular phylogenetic studies revealed the extraordinary diversity of single-celled eukaryotes. however, the proper assessment of this diversity and accurate reconstruction of the eukaryote phylogeny are still impeded by the lack of molecular data for some major groups of easily identifiable and cultivable protists. among them, amoeboid eukaryotes have been notably absent from molecular phylogenies, despite their diversity, complexity, and abundance. to partly fill this phylogenetic gap, ... | 2004 | 15148395 |
| nickel trafficking: insights into the fold and function of uree, a urease metallochaperone. | uree is a metallo-chaperone assisting the incorporation of two adjacent ni(2+) ions in the active site of urease. this study describes an attempt to distill general information on this protein using a computational post-genomic approach for the understanding of the structural details of the molecular function of uree in nickel trafficking. the two crystal structures recently determined for uree from bacillus pasteurii (bpuree) and klebsiella aerogenes (kauree) were comparatively analyzed. this a ... | 2004 | 15134926 |
| antimicrobial and antioxidative enrichment of oak (quercus robur) bark by rotation planar extraction using extrachrom. | the multifunctional extrachrom instrument was used in the extraction of antimicrobial and radical scavenging components from oak (quercus robur l.) bark. milled and sieved oak bark was extracted with 80% (v/v) methanol solution in water on the extrachrom instrument using step-gradient in the preparative separation. extracts were tested using agar diffusion method on staphylococcus aureus, enterobacter aerogenes and candida albicans. some extracts showed moderate bactericidal, fungicidal, bacteri ... | 2004 | 15109795 |
| purification and some properties of superoxide dismutase from deinococcus radiophilus, the uv-resistant bacterium. | the superoxide dismutase (sod, ec 1.15.1.1) of deinococcus radiophilus, a bacterium extraordinarily resistant to uv, ionizing radiations, and oxidative stress, was purified 1,920-fold with a 58% recovery yield from the cell-free extract of stationary cells by steps of ammonium sulfate fractionation and superdex g-75 gel-filtration chromatography. a specific activity of the purified enzyme preparation was ca. 31,300 u mg(-1) protein. d. radiophilus sod is mn/fesod, judging by metal analysis and i ... | 2004 | 15106001 |