Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| genetic transformation of strawberry by agrobacterium tumefaciens using a leaf disk regeneration system. | an efficient genetic transformation protocol has been developed for strawberry cv. redcoat using agrobacterium tumefadens. the protocol relies on a high frequency (84%) shoot regeneration system from leaf disks. the leaf disks were inoculated with a non-oncogenic agrobacterium tumefadens strain mp90 carrying a binary vector plasmid pbi121 which contains a chimeric nopaline synthase (nos) promoter driven neomycin phosphotransferase (npt ii) gene and a cauliflower mosaic virus 35s (camv35s) promot ... | 1990 | 24226936 |
| agrobacterium tumefaciens-mediated transformation of pisum sativum l. using binary and cointegrate vectors. | epicotyl segments and nodus expiants from etiolated seedlings of pisum sativum were transformed using agrobacterium tumefaciens strains gv 2260 (p35s gus int) and gv 3850 hpt carrying either a neomycin- or hygromycinphosphotransferase-gene as selectable markers. the transgenic character of hygromycin- or kananamycin-resistant tissue was confirmed by detection of nopaline or neomycinphosphotransferase-ii- and ß-glucuronidase activity in crude extracts of resistant tissues. up to 5 % of developing ... | 1990 | 24226826 |
| transformation of cucumis sativus tissue by agrobacterium tumefaciens and the regeneration of transformed plants. | cotyledons of cucumber seedlings (cucumis sativus l. cv. poinsett 76) were co-cultivated with disarmed agrobacterium strain c58z707. the agrobacterium strain contained the agrobacterium-derived binary vector plasmid pga482, its t-dna region contains a plant expressible bacterial derived neomycin phosphotransferase ii (npt ii) gene which upon transfer, genome integration, and expression in plant tissues confers resistance to the antibiotic kanamycin. after growth of inoculated cotyledon sections ... | 1990 | 24226818 |
| transgenic brassica napus plants obtained by cocultivation of protoplasts with agrobacterium tumefaciens. | hypocotyl protoplasts of german winter oilseed, rape (brassica napus) lines of double-low quality were transformed using agrobacterium tumefaciens harbouring pgv 3850∶1103 neo (dimer) containing chimaeric kanamycin resistance reporter genes. transformed protoplasts were regenerated to fertile and phenotypically normal plants. transformation was confirmed by kanamycin resistance, nopaline production, neomycinphosphotransferase ii activity, and southern blot hybridization. seed progeny from self-p ... | 1990 | 24226815 |
| beet necrotic yellow vein virus coat protein-mediated protection in sugarbeet (beta vulgaris l.) protoplasts. | transformed beta vulgaris l. suspension cultures were obtained after cocultivation of sugarbeet cells with agrobacterium tumefaciens harbouring a binary vector containing the coat protein gene of beet necrotic yellow vein virus inserted between the kanamycin resistance gene and a ß-glucuronidase reporter gene. protoplasts were isolated both from untransformed cells, and from transformed cells expressing the viral coat protein, and both were then infected with beet necrotic yellow vein virus. com ... | 1990 | 24226708 |
| agrobacterium tumefaciens mediated transformation and regeneration of muskmelon plants. | transgenic muskmelon (cucumis melo l.) plants were produced efficiently by inoculating cotyledon explants with agrobacterium tumefaciens strain lba4404 bearing a ti plasmid with the npt ii gene for kanaymcin resistance. after co-cultivation for three days, expiants were transferred to melon regeneration medium with kanamycin to select for transformed tissue. shoot regeneration occurred within 3-5 weeks; excised shoots were rooted on medium containing kanamycin before transferring to soil. morpho ... | 1990 | 24226603 |
| within-population variation in susceptibility to agrobacterium tumefaciens a281 in picea abies (l.) karst. | the purpose of this study was to investigate the genetic variation of susceptibility to agrobacterium within a picea abies population. tumor formation was studied in 16 open-pollinated families belonging to a central swedish population of picea abies. strain a281 of agrobacterium tumefaciens was used to infect 3-monthold seedlings in a five-block greenhouse experiment. the analysis of variance showed strong significance for the between-family variation of tumor-formation percentages, varying fro ... | 1990 | 24226580 |
| meristem transformation of sunflower via agrobacterium. | for transformation of sunflower (helianthus annuus l. cv. zebulon), shoot apical meristems were dissected from seeds and cocultivated with a disarmed agrobacterium tumefaciens strain harboring a binary vector carrying genes encoding gus- and nptii-activity. the influence of the media conditions, the time of cocultivation and the stage of the developing seed on shoot development and meristem transformation was analysed. transformants were selected by their ability to grow on kanamycin. transforma ... | 1990 | 24226429 |
| transformation of eggplant (solanum melongena l.) using a binary agrobacterium tumefaciens vector. | kanamycin resistant plants of solarium melongena l. (eggplant) cv. picentia were obtained following the cocultivation of leaf explants with agrobacterium tumefaciens. a disarmed binary vector system containing the neomycin phosphotransferase (nptii) gene as the selectable marker and chloramphenicol acetyltransferase (cat) as a reporter gene was utilized. in vitro grown plants were used as sources of explants to produce transgenic plants on selective medium containing 100 mg/l kanamycin. the tran ... | 1990 | 24226372 |
| agrobacterium-mediated transformation of strawberry calli and recovery of transgenic plants. | transformed calli and shoots of strawberry (fragaria × ananassa duch.) cv. redcoat were obtained using agrobacterium tumefaciens carrying plasmid pb1121. inoculated leaf explants produced transgenic calli at a frequency of 3% on selection medium containing 50 μg/ml kanamycin. twenty per cent of selected caili regenerated, giving rise to transgenic shoots. all transgenic calli and shoots expressed substantial amounts of gus and npt-ii activity. the southern blot analysis confirmed the insertion o ... | 1990 | 24226368 |
| agrobacterium-mediated transformation of solanum tuberosum l. cv. 'russet burbank'. | stem sections from shoot cultures maintained in vitro were used to produce transgenic plants of the potato, solanum tuberosum l. cv. 'russet burbank'. stem internode pieces inoculated with agrobacterium tumefaciens containing coat protein genes from potato virus x and potato virus y, produced shoots with a frequency of 60% in the absence of selection and 10% on medium containing 100 mg/l kanamycin monosulfate. regenerated shoots were assayed for kanamycin resistance by placing stem segments on c ... | 1991 | 24226160 |
| the pseudomonas savastanoi tryptophan-2-mono-oxygenase is biologically active in nicotiana tabacum. | it has been proposed that the "eukaryotic" t-dna-encoded indole-3-acetic acid (iaa) biosynthesis genes of agrobacterium tumefaciens and their prokaryotic counterpart in pseudomonas savastanoi originated from common ancestor genes. this paper provides additional evidence for the functional similarity between the gene products. we have demonstrated that a chimeric gene consisting of the coding sequence of the p. savastanoi tryptophan-2-mono-oxygenase (iaam gene) and a plant promoter encodes an act ... | 1987 | 24226077 |
| auxin-induced rapid changes in translatable mrnas in tobacco cell suspension. | when 2,4-dichlorophenoxyacetic acid (2,4-d)-dependent tobacco cell suspensions, one normal and one transformed by agrobacterium tumefaciens, were subcultured on hormone-lacking medium the stationary phase of the cell cycle was reached earlier than on medium containing 2,4-d. addition of the auxin 2,4-d could restore cell division activity within 10-12 h for the most rapidly reacting cell line. the cell-division response was characterized as being auxin-specific and optimal with 2,4-d at 2.2 10(- ... | 1987 | 24226071 |
| haploid transformation in brassica napus using an octopine-producing strain of agrobacterium tumefaciens. | microspore-derived embryos of brassica napus were transformed using the disarmed octopine-producing lba4404 strain of agrobacterium tumefaciens containing the binary vector pbin19. octopine-producing strains have previously been reported to be ineffective in transforming brassica. four actively growing yellow/ green sectors were selected from the embryos on 50 mg/l kanamycin and plants regenerated. analysis for npt-ii activity in these young plants initially indicated no expression of the bacter ... | 1989 | 24226014 |
| initiation of auxin autonomy in nicotiana glutinosa cells by the cytokinin-biosynthesis gene from agrobacterium tumefaciens. | agrobacteria carrying mutations at the auxin-biosynthesizing loci (iaah and iaam of the ti plasmid) induce shoot-forming tumors on many plant species. in some cases, e.g. nicotiana glutinosa l., tumors induced by such mutant strains exhibit an unorganized and fully autonomous phenotype. these characteristics are stable in culture at both the tissue and cellular level. we demonstrate that the cytokinin-biosynthesis gene (ipt) of the ti plasmid is responsble for the induction of both auxin and cyt ... | 1987 | 24225718 |
| efficient transformation of potato (solanum tuberosum l.) using a binary vector in agrobacterium rhizogenes. | we transformed three potato (solanum tuberosum l.) genotypes by using a. rhizogenes or a mixture of a. rhizogenes and a. tumefaciens. inoculations of potato stem segments were performed with agrobacterium rhizogenes am8703 containing two independent plasmids: the wild-type ri-plasmid, pri1855, and the binary vector plasmid, pbi121. in mixed inoculation experiments, agrobacterium rhizogenes lba1334 (pri1855) and agrobacterium tumefaciens am8706 containing the disarmed ti-plasmid (pal4404) and the ... | 1989 | 24225690 |
| growth and disinfestation of 6 different bacteria in embryogenic suspension cultures of cotton. | growth of 6 different common laboratory bacteria (escherichia coli, flavobacterium balustrum, xanthomonas maltophilia, enterobacter cloacae, pseudomonas fluorescens, and agrobacterium tumefaciens) in a bacterial medium, fresh plant medium, and "spent" plant media was initially measured. in all cases, bacteria grew best in the bacterial medium followed by the fresh plant medium. the spent plant medium did not support growth of the bacteria and apparently was actively toxic to bacterial cells. pro ... | 1991 | 24221727 |
| gene transfer into peanut (arachis hypogaea l.) by agrobacterium tumefaciens. | introduction of foreign genes into plant tissues via agrobacterium tumefaciens based vectors requires specific knowledge of agrobacterium-host compatibility. therefore, to develop a transformation protocol for peanut (arachis hypogaea l.), five brazilian cultivars were screened with four wild-type a.tumefaciens strains. successful transformation was dependent on specific bacterial strain-plant cultivar interactions and strain a281 was the most effective for tumor induction. tumors displayed horm ... | 1991 | 24221673 |
| gene transfer in plants of brassica juncea using agrobacterium tumefaciens-mediated transformation. | an efficient system for gene transfer into plants of brassica juncea var. india mustard, mediated by agrobacterium tumefaciens. was developed through the manipulation of the culture medium and the use of the appropriate agrobacterium strain. high frequency shoot regeneration (90-100%) was obtained from hypocotyl explants grown on medium containing 0.9% agarose, 3.3 mg/l agno3 and 0.5-2 mg/l ba in combination with 0.01-0.05 mg/l 2,4-d or 0.1-1 mg/l naa. of all the agrobacterium strains tested, a. ... | 1991 | 24221663 |
| agrobacterium-mediated transformation and regeneration of kiwi fruit. | genetically transformed kiwi fruit (actinidia deliciosa) plants were obtained from hypocotyl and stem segments co-cultured with agrobacterium tumefaciens strain eha101 harboring a binary vector, plan411 or plan421, which contained the neomycin phosphotransferase ii (nptii) gene and the β-glucuronidase (gus) gene. after co-culturing with the a. tumefaciens, the hypocotyl or stem segments were cultured on a selection medium containing 25μg/ml kanamycin and 500μg/ml claforan. after one month in cul ... | 1991 | 24221658 |
| agrobacterium-mediated transformation of pepino and regeneration of transgenic plants. | regeneration of pepino (solanum muricatum ait.) shoots was achieved both by organogenesis and by embryogenesis. shoots derived via organogenesis were easily rooted and most regenerated plants appeared phenotypically normal. transgenic plants were obtained using the binary vector pkiwi110 in the avirulent agrobacterium tumefaciens strain lba4404. optimization of transformation protocols was rapidly achieved by monitoring early expression of the gus (β-d-glucuronidase) reporter gene carried on pki ... | 1991 | 24221548 |
| regeneration and agrobacterium-mediated transformation of chrysanthemum. | a method has been developed to regenerate shoots directly from leaf pieces of the autumn flowering chrysanthemum dendranthema indicum (l.) des moul (genotype korean). transgenic plants of this genotype were generated using transformation mediated by the disarmed strain of agrobacterium tumefaciens lba4404, containing either pkiwi110 or pga643. both pkiwi110 and pga643 contain the selectable marker gene neomycin phosphotransferase ii (nptii) and pkiwi110 also contains the reporter gene β-d-glucur ... | 1991 | 24221545 |
| effect of culture conditions on agrobacterium-mediated transformation in datura. | a two step selection procedure is described for high frequency transformation and regeneration of transgenic plants by coculture of leaf discs of datura innoxia with agrobacterium tumefaciens carrying binary vectors. leaf discs were cocultured with disarmed a. tumefaciens vectors pgs glucl, pgstrn943, pgv2260 and pbi121, and subcultured on regeneration media containing kanamycin. kanamycinresistant, putatively "transformed" callus and vegetative buds were isolated, and subcultured on media conta ... | 1991 | 24221401 |
| somatic hybridization between selected lycopersicon and solanum species. | mesophyll protoplasts of an interspecific lycopersicon esculentum mill, (tomato) x lycopersicon pennellii hybrid plant (ep) were fused with callus-derived protoplasts of solanum lycopersicoides dun. using a modified peg/dmso procedure. the ep plant was previously transformed by agrobacterium tumefaciens which carried the nptii and nopaline synthase genes. protoplasts were plated at 10(5)/ml in modified km medium and 16 days post-fusion 25 ug/ml kanamycin was added to the culture medium. during s ... | 1991 | 24221398 |
| pemu: an improved promoter for gene expression in cereal cells. | a recombinant promoter, pemu, has been constructed to give a high level of gene expression in monocots. it is based on a truncated maize adh1 promoter, with multiple copies of the anaerobic responsive element from the maize adh1 gene and ocs-elements from the octopine synthase gene of agrobacterium tumefaciens. the pemu promoter was one of 12 different promoter constructs that were linked to the β-glucuronidase (gus) marker gene. promoter activity was measured 48 h after introduction of the cons ... | 1991 | 24221371 |
| patterns of transformation intensity on flax hypocotyls inoculated with agrobacterium tumefaciens. | cellular transformation intensities on flax (linum usitatissimum) hypocotyl explants using disarmed agrobacterium tumefaciens were investigated through various preculture durations, cocultivation durations and removal of epidermis. the expression of an intron-containing β-glucuronidase (gus) gene driven by camv 35s promoter served as a reporter for determination of transformed tissues on hypocotyls. the binary plasmid p35sgusint in octopine-type agrobacterium strain gv2260 was used as the vector ... | 1991 | 24221329 |
| induction of virulence response in agrobacterium tumefaciens by tissue explants of various plant species. | forty-four plant species belonging to different taxa were tested for their ability to induce the expression of the virulence e gene in agrobacterium tumefaciens containing vire:lacz fusion constructs. with the exception of 6 algae, one fern and 2 monocots, tissue explants of all other plants (2 algae, 3 bryophytes, 2 pteridophytes, 15 gymnosperms, 8 monocots and 5 dicots) induced the expression of the vire gene as detected by the presence of β-galactosidase activity in the bacteria. | 1991 | 24221327 |
| crown gall transformation of lentil (lens culinaris medik.) with virulent strains of agrobacterium tumefaciens. | four diverse strains of agrobacterium tumefaciens (c58, ach5, gv3111, and a281) were capable of inducing tumors at a high frequency on inoculated stems of lentil (lens culinaris medik. cultivar laird) in vivo, and on excised shoot apices in vitro. gv3111 and ach5 produced the largest and heaviest tumors in vivo, while a281 produced the heaviest tumors in vitro. tumor formation and opine production are indicative of plant cell transformation and tumors produced appropriate opines: nopaline (c58), ... | 1991 | 24221280 |
| insertional mutagenesis in arabidopsis thaliana: isolation of a t-dna-linked mutation that alters leaf morphology. | we investigated the potential of the agrobacterium tumefaciens t-dna as an insertional mutagen in arabidopsis thaliana. arabidopsis lines transformed with different t-dna vectors were generated using a leaf disc infection procedure adapted for efficient selection on either kanamycin or hygromycin medium. a standardized screening procedure was developed for the detection of recessive mutations in t2 populations of regenerated and/or transformed lines. recessive mutations originating from the tiss ... | 1991 | 24221214 |
| production of transgenic pea (pisum sativum l.) plants by agrobacterium tumefaciens - mediated gene transfer. | a transformation system that allows regeneration of transgenic pea plants from calli selected for antibiotic resistance was developed. explants from axenic shoot cultures and seedling epicotyls were cocultivated with nononcogenic agrobacterium tumefaciens strains, and transformed callus could be selected on callus-inducing media containing either 15 mg/l hygromycin or 75 mg/l kanamycin. after several passages on regeneration medium, shoot organogenesis could be reproducibly induced on hygromycin ... | 1990 | 24220903 |
| genetic characterization of a double-flowered tobacco plant obtained in a transformation experiment. | a leaf-disk transformation experiment was performed with tobacco (nicotiana tabacum l.) using a binary vector and a strain of agrobacterium tumefaciens that carried a wild-type ti-plasmid, ptibo542. although the majority of kanamycin-resistant, transgenic plants was morphologically normal, one of the plants was double-flowered and had a slightly wavy stem and leaves whose edges were bent slightly upwards. the abnormal morphology was controlled by a single, dominant mendelian gene. young plants t ... | 1990 | 24220890 |
| mass-spectrometric quantitation of cytokinins in tobacco crown-gall tumours induced by mutated octopine ti plasmids of agrobacterium tumefaciens. | the levels of the major cytokinins, zeatin, zeatin riboside, zeatin riboside-5'-monophosphate and zeatin-7-glucoside were measured in tobacco (nicotiana tabacum l.) crown-gall tissues carrying insertion and deletion mutations in the t-dna. measurements were made by combined gas chromatography-mass spectrometry using selected ion monitoring with (15)n- and (2)h-labelled internal standards. the results demonstrate that, relative to wild-type tumour tissue, cytokinin levels are considerably elevate ... | 1988 | 24220777 |
| transformation of vicia narbonensis via agrobacterium-mediated gene transfer. | shoot tips and epicotyl-segments of vicia narbonensis were co-cultivated with agrobacterium tumefaciens strain c58c1 pgv 3850 hpt, carrying a plasmid coding for hygromycin-phosphotransferase. on callus-induction medium containing 60 mg/l hygromycin for selection, approximately 18% of the explants produced hygromycin-resistant callus. after transfer to regeneration-medium these calluses produced hygromycin-resistant and nopaline-positive somatic embryos which could be regenerated to plantlets. th ... | 1991 | 24220706 |
| threats and opportunities of plant pathogenic bacteria. | plant pathogenic bacteria can have devastating effects on plant productivity and yield. nevertheless, because these often soil-dwelling bacteria have evolved to interact with eukaryotes, they generally exhibit a strong adaptivity, a versatile metabolism, and ingenious mechanisms tailored to modify the development of their hosts. consequently, besides being a threat for agricultural practices, phytopathogens may also represent opportunities for plant production or be useful for specific biotechno ... | 2014 | 24216222 |
| optimizing the production of transformed pea (pisum sativum l.) callus using disarmed agrobacterium tumefaciens strains. | for optimization of the transformation procedure with pisum sativum l. stern explant callus was used to test the effect of disarmed agrobacterium tumefaciens strains, cocultivation procedures (preconditioning of explants; use of nicotiana tabacum l. nurse cultures), duration of cocultivation (2, 3 or 4 days), and agents for selection (kanamycin or hygromycin). the succinamopine strain eha101(pbi1042) produced the highest percentage of transformed calli (77%) when used in conjunction with tobacco ... | 1991 | 24213784 |
| a rapid and efficient regeneration system for pea (pisum sativum), suitable for transformation. | a method for plant regeneration via organogenesis in pea (pisum sativum) using nodal thin cell layer segments has been developed.from 10 to 12 days old sterile pea seedlings, nodal expiants were excised from which leaves and axillary buds were removed. shoot regeneration was consistently obtained from liquid cultures where the expiants were floated on the medium. shoots could be harvested after two weeks and thereafter up to ten weeks and no important effect of the cultivar (bodil, puget, rondo ... | 1991 | 24213691 |
| the effects of acetosyringone and ph on agrobacterium-mediated transformation vary according to plant species. | expiants of five plant species (allium cepa, antirrhinum majus, brassica campestris. glycine max, and nicotiana tabacum) were co-cultivated with three agrobacterium tumefaciens strains under different conditions to assess the effects of acetosyringone and medium ph on strain virulence. tumours were incited on all dicotyledonous species by strains n2/73 and a281. the presence of acetosyringone during co-cultivation generally enhanced the virulence of these strains, most markedly n2/73 on a. majus ... | 1991 | 24213690 |
| transgenic poplars: expression of chimeric genes using four different constructs. | leaf or stem explants of a hybrid poplar clone (populus tremula x populus alba), sensitive to agrobacterium tumefaciens, were co-cultivated either by an octopine or a nopaline disarmed a. tumefaciens modified strain. transformed poplar shoots were readily regenerated from explants. the protocol was improved using the nopaline disarmed strain c58/pmp90 with the binary vector pbi121. this protocol was then used to test three other vectors. the first one, possessing a nptii gene fused to the camv 1 ... | 1992 | 24213546 |
| agrobacterium tumefaciens-mediated transformation of safflower (carthamus tinctorius l.) cv. 'centennial'. | efficient callus formation was achieved from cotyledon, stem, and leaf expiants of the domestic safflower cultivar 'centennial' on ms salts medium containing 1 mg/l bap and 1 mg/l naa. shoot buds were regenerated from 26% of leaf-derived calli on callus induction medium, although attempts to root regenerated shoots were not successful. 'centennial' expiants inoculated with agrobacterium tumefaciens containing npt ii and gus genes produced kanamycin-resistant calli from which buds were regenerate ... | 1992 | 24213291 |
| transformation and regeneration of brassica rapa using agrobacterium tumefaciens. | transformation and regeneration procedures for obtaining transgenic brassica rapa ssp. oleifera plants are described. regeneration frequencies were increasedby using silver nitrate and by adjusting the duration of exposure to 2,4-d. for transformation, agrobacterium tumefaciens strain eha101 containing a binary plasmid with the neomycin phosphotransferase gene (npt ii) and the b-glucuronidase gene (gus) was cocultivated with hypocotyl explants from the oilseed b. rapa cvs. tobin and emma. transf ... | 1992 | 24213157 |
| expression of pvx coat protein gene under the control of extensin-gene promoter confers virus resistance on transgenic potato plants. | tuber discs of potato (solanum tuberosum l.) cultivars desirée and gracia were infected by agrobacterium tumefaciens carrying a binary vector with the coat protein gene of potato virus x controlled by the carrot extensin gene long-transcript promoter. several transgenic potato plants have been obtained by direct regeneration of shoots on culture medium with kanamycin used for selection. the presence of the coat protein gene was proved by southern hybridization in several transformants. its low b ... | 1992 | 24213037 |
| coat protein mediated resistance to plum pox virus in nicotiana clevelandii and n. benthamiana. | transgenic nicotiana benthamiana and n. clevelandii plants expressing the coat protein of plum pox virus under the control of the 35s promoter from cauliflower mosaic virus were engineered by agrobacterium tumefaciens mediated transformation. the phenomenon of virus resistance was observed at different levels when transgenic plants, expressing the coat protein and control plants were compared after challenge infection with plum pox virus. n. clevelandii coat protein transgenic plants circumvent ... | 1992 | 24213033 |
| regeneration of transgenic plants of prunus armeniaca containing the coat protein gene of plum pox virus. | a system was developed which allows the transfer of foreign genes into apricot cultivars. we report the transformation and regeneration of prunus armeniaca plants with agrobacterium tumefaciens strain lba 4404 containing various binary plasmids, pbingusint, carrying the marker gene ß-glucuronidase (gus) and pbinppvm, carrying the coat protein gene of plum pox virus (ppv). the marker gene gus was used for optical evaluation of the efficiency of the transformation system. the coat protein gene of ... | 1992 | 24213032 |
| introduction and differential use of various promoters in pollen grains of nicotiana glutinosa and lilium longiflorum. | as part of our research to develop an alternative system for the transformation of recalcitrant plant species we investigated the use of the male gametophyte as a transformation vector. therefore the activity of four different promoters (camv 35s, lat52, chia pa2 and tr2') was analyzed in pollen of a dicot (nicotiana glutinosa) and a monocot (lilium longiflorum) plant species. gene constructs in which the ß-glucuronidase (gus) gene was placed under the control of these promoters were introduced ... | 1992 | 24213031 |
| transformation of solanum integrifolium poir via agrobacterium tumefaciens: plant regeneration and progeny analysis. | the wild species solanum integrifolium represents a source of pest and disease resistance genes for breeding strategies of the cultivated species solanum melongena. somatic hybridization via protoplast fusion between the two species may provide a valuable tool for transferring polygenic traits into the cultivated species. the availability of s.integrifolium cells carrying dominant selectable markers would facilitate the heterokaryon rescue. an appropriate methodology for in vitro culture and pla ... | 1992 | 24213029 |
| sites of synthesis, translocation and accumulation of pyrrolizidine alkaloid n-oxides in senecio vulgaris l. | (14)c-labelled alkaloid precursors (arginine, putrescine, spermidine) fed to senecio vulgaris plants via the root system were rapidly taken up and efficiently incorporated into the pyrrolizidine alkaloid senecionine n-oxide (sen-nox) with total incorporations of 3-6%. considerable amounts of labelled sen-nox were translocated into the shoot and were directed mainly into the inflorescences, the major sites of pyrrolizidine-alkaloid accumulation. detached shoots of s. vulgaris were unable to synth ... | 1989 | 24212277 |
| identification and characterization of granule bound starch synthase i (gbssi) gene of tartary buckwheat (fagopyrum tataricum gaertn.). | tartary buckwheat (fagopyrum tataricum gaertn.) is increasingly considered as an important functional food material because of its rich nutraceutical compounds. reserve starch is the major component of tartary buckwheat seed. however, the gene sequences and the molecular mechanism of tartary buckwheat starch synthesis are unknown so far. in this study, the complete genomic sequence and full-size cdna coding tartary buckwheat granule-bound starch synthase i (ftgbssi), which is responsible for amy ... | 2014 | 24211386 |
| pigment-protein interactions in phytochromes probed by fluorescence line narrowing spectroscopy. | fluorescence line narrowing (fln) spectroscopy was used to study bacteriophytochromes and variants from various species in their red-absorbing pr ground state, including phytochromes agp1 from agrobacterium tumefaciens , drbphp from deinococcus radiodurans , and rpbphp2 and rpbphp3 from rhodopseudomonas palustris . a species-dependent narrowing of the fluorescence emission bands is observed. the results suggest varied pigment-protein interactions, possibly connected to chromophore mobility or ex ... | 2013 | 24205818 |
| inheritance of a bacterial hygromycin phosphotransferase gene in the progeny of primary transgenic pea plants. | an analysis of the progeny of primary transgenic pea plants in terms of transmission of the transferred dna, fertility and morphology is presented. a transformation system developed for pea that allows the regeneration of fertile transgenic pea plants from calli selected for antibiotic resistance was used. expiants from axenic shoot cultures were co-cultivated with a nononcogenic agrobacterium tumefaciens strain carrying a gene encoding hygromycin phosphotransferase as selectable marker, and tra ... | 1992 | 24203206 |
| agrobacterium tumefaciens-mediated beta-glucuronidase (gus) gene expression in lentil (lens culinaris medik.) tissues. | lentil (lens culinaris medik.) shoot apex, epicotyl, and root expiants were capable of expressing an intron-containing beta-glucuronidase (gus) gene after inoculation with the disarmed agrobacterium strain gv2260:p35sgusint. expression occurred at all wound sites on these expiants except at the end of the root expiants proximal to the cotyledonary node. gus expression was detected using both histochemical and fluorescence assays and was stable for at least nine days after inoculation for epicoty ... | 1992 | 24203139 |
| agrobacterium mediated transfer of a mutant arabidopsis acetolactate synthase gene confers resistance to chlorsulfuron in chicory (cichorium intybus l.). | leaf discs of c. intybus were inoculated with an agrobacterium tumefaciens strain harboring a neomycin phosphotransferase (neo) gene for kanamycin resistance and a mutant acetolactate synthase gene (csr1-1) from arabidopsis thaliana conferring resistance to sulfonylurea herbicides. a regeneration medium was optimized which permitted an efficient shoot regeneration from leaf discs. transgenic shoots were selected on rooting medium containing 100 mg/l kanamycin sulfate. integration of the csr1-1 g ... | 1992 | 24203132 |
| factors influencing the efficiency of t-dna transfer during co-cultivation of antirrhinum majus with agrobacterium tumefaciens. | the effects of varying the ph of the cocultivation medium, additons of vir-inducing phenolic compounds and the strains of wild-type agrobacteria on transformation rates of a number of different varieties of antirrhinum majus were studied. in general, optimal transformation was found with strains c58 or a281 and was favoured by low ph and the inclusion of acetosyringone in the co-cultivation medium. however, maximal transformation of the least susceptible variety was achieved at high ph and in th ... | 1992 | 24202985 |
| transformation of vitis tissue by different strains of agrobacterium tumefaciens containing the t-6b gene. | stem pieces and leaf disks of vitis spp. were cocultured with agrobacterium tumefaciens strains carrying the uida (ß-glucuronidase = gus) gene. the transformation efficiency was highly increased by using a modified t-6b gene (a gene from ptitm4) which interferes with normal growth and allows regeneration of normal nicotiana rustica plants (tinland 1990). the strains first tested on stem segments were subsequently tested in a leaf explant system. on leaves the transformation efficiency of the str ... | 1992 | 24202984 |
| cucumber mosaic virus-tolerant transgenic tomato plants expressing a satellite rna. | a satellite rna (t73-satrna) gave reduced symptom severity on tomato plants when coinoculated with an ordinary strain of cucumber mosaic virus (cmvo). cdna for t73-satrna was introduced into a binary vector (ptok162) through a homologous recombination in an agrobacterium tumefaciens cell, and then transferred to leaf disks of tomato. stable integration and transcription of the cdna in the regenerants were verified by southern and northern blot hybridizations, respectively. upon inoculation with ... | 1992 | 24202740 |
| genetic transformation of green bean callus via agrobacterium mediated dna transfer. | kanamycin resistant callus was produced from leaf disc or hypocotyl expiants of green bean (phaseolus vulgaris l.) when cultured on a defined medium containing 50 mg/l kanamycin after 4 days of co-cultivation with agrobacterium tumefaciens strain eha101 containing the binary vector pkylx71gus. the presence of neomycin phosphotransferase ii (npt-ii) in crude cellular extracts from the kanamycin resistant callus was confirmed by elisa. the expression of the ß-glucuronidase (gus) reporter gene was ... | 1993 | 24202072 |
| transgenic plants of ramie (boehmeria nivea gaud.) obtained by agrobacterium mediated transformation. | a regeneration and transformation protocol for ramie (boehmeria nivea gaud.) is presented. regeneration was obtained from leaf discs placed on solid b-5 medium (gamborg et al. 1968) containing adequate concentrations of auxin and cytokinin. co-cultivation of leaf discs with agrobacterium tumefaciens and subsequent regeneration resulted in transgenic plants as shown by southern blot and analysis of expression of the gus-marker gene. | 1993 | 24201876 |
| effect of acetone in the culture medium on the regeneration efficiency and on the resistance to root rot of regenerants of various plant species. | various expiants of wheat, onion, garlic, and cabbage were examined for their regeneration efficiency in the presence of acetone in the culture medium. acetone at concentrations between 1.5% and 3.0% (w/v) was shown to increase the degree of callusing and meristem formation, as well as the capacity to regenerate. it also significantly increased the survival and regeneration efficiency of expiants inoculated with agrobacterium tumefaciens, a bacterium known to reduce expiant viability. resistance ... | 1993 | 24201789 |
| acetosyringone and osmoprotectants like betaine or proline synergistically enhance agrobacterium-mediated transformation of apple. | the effects of the plant signal molecule acetosyringone (as) and the osmoprotectant betaine phosphate (bp) have been examined for their ability to increase the transformation efficiency of agrobacterium tumefaciens (at), c58c1::pgv3850 harboring the binary vector pkiwi105. this binary plasmid encodes the β-glucuronidase (gus) gene and was previously shown to be expressed exclusively in plant tissues. bacteria were grown in one of two previously reported virulence induction media (ms20 and sim) f ... | 1993 | 24201785 |
| composition of the microflora of witloof chicory seeds. | the bacterial microflora of nine varieties of witloof chicory (cichorium intybus l. var.foliosum hegi) seeds was studied. the 184 isolates were characterized by protein profiles determined by sds-protein polyacrylamide gel electrophoresis of the total cell proteins. isolates with identical protein profiles were grouped into one fingerprint type. sixty-seven fingerprint types were distinguished. two quantitatively major fingerprint types,erwinia herbicola and an arthrobacter, represented 52% of t ... | 1988 | 24201719 |
| shoot regeneration in stem expiants and its amenability to agrobacterium tumefaciens mediated gene transfer in brassica carinata. | immature stem segments of seven different genotypes of brassica carinata produced shoots with variable frequencies when cultured in ms medium with bap and picloram at 0.2 mg/l each. line 171, which produced shoots with 100% efficiency from both cut ends of the expiant, was selected for testing the amenability of this regeneration protocol for genetic transformation. a non-oncogenic agrobacterium tumefaciens containing plasmid pcv 730, a binary vector carrying resistance genes for kanamycin and h ... | 1992 | 24201439 |
| thiophene accumulation in relation to morphology in roots of tagetes patula : effects of auxin and transformation by agrobacterium. | roots of marigold (tagetes patula l.) accumulate thiophenes, heterocyclic sulfurous compounds with strong biocidal activity. in detached roots cultured in vitro, the thiophene content was 5 μmol·(g fresh weight)(-1) which is 25-times higher than in roots attached to the plant. in roots derived from tissues transformed by agrobacterium tumefaciens and a. rhizogenes, the morphology and thiophene content varied with the bacterial strain used. transformation stimulated the elongation of the root tip ... | 1989 | 24201420 |
| enhanced transformation of tomato co-cultivated with agrobacterium tumefaciens c58c1rif(r)::pgsfr1161 in the presence of acetosyringone. | explants of tomato (lycopersicon esculentum mill cv. ailsa craig) were co-cultivated with agrobacterium tumefaciens c58c1rif(r)::pgsfr1161 in the presence of 20 (μm acetosyringone). transformed root clones were selected on kanamycin medium and the presence of the nptii gene in the plant dna confirmed by the polymerase chain reaction. root clones derived from acetosyringone treatment grew more vigorously in the presence of kanamycin and synthesized a greater amount of npt-ii enzyme. the conclusio ... | 1993 | 24197345 |
| a modified method for routine agrobacterium-mediated transformation of in vitro grown potato microtubers. | in vitro-grown potato (solanum tuberosum l.) microtubers were used as an explant source in the production of transgenic plants by agrobacterium-mediated gene transfer. in this study we tested four diverse potato cultivars, lemhi russet, russet burbank, wauseon, and yankee chipper on various levels of zeatin riboside and 3-indoleacetyl-dl-aspartic acid for their ability to regenerate transgenic plants after infection with agrobacterium tumefaciens. culturing microtuber blocks from the medullary a ... | 1993 | 24197257 |
| transformation of liquidambar styraciflua using agrobacterium tumefaciens. | we describe the molecular transformation of liquidambar styraciflua using agrobacterium tumefaciens. a binary ti-plasmid vector containing a chimeric neomycin phosphotransferagene which confers resistance to kanamycin and either a chimeric bacillus thuringiensis toxin gene, a chimeric e. coli β-glucuronida(gus), or a chimeric tobacco anionic peroxidase gene was introduced into sweetgum by co-cultivation with agrobacterium tumefaciens. sweetgum shoots regenerated in the presence of kanamycin were ... | 1993 | 24197252 |
| transgenic papaya plants from agrobacterium-mediated transformation of somatic embryos. | transgenic papaya (carica papaya l.) plants were regenerated from embryogenic cultures that were cocultivated with a disarmed c58 strain of agrobacterium tumefaciens containing one of the following binary cosmid vectors: pga482gg or pga482gg/cpprv-4. the t-dna region of both binary vectors includes the chimeric genes for neomycin phosphotransferase ii (nptii) and ß-glucuronidase (gus). in addition, the plant expressible coat protein (cp) gene of papaya ringspot virus (prv) is flanked by the npti ... | 1993 | 24197150 |
| metabolic factors capable of inducing agrobacterium vir gene expression are present in rice (oryza sativa l.). | the effects of exudates and extracts from suspension cultures or various parts of rice (oryza sativa l.) plants on induction of vir (virulence) gene expression in agrobacterium tumefaciens were examined. only leaf extracts from panicle-differentiating plants to flowering plants were able to strongly induce activation and expression of vir genes. this induction was similar to that observed with 2 μm acetosyringone (as), yet there was no synergy between as and rice extracts. responses to vir-induc ... | 1993 | 24196854 |
| agrobacterium-mediated transformation of asparagus officinalis l. long-term embryogenic callus and regeneration of transgenic plants. | twenty-three independent kanamycin resistant lines were obtained after cocultivation of longterm embryogenic cultures of three asparagus officinalis l. genotypes with an agrobacterium tumefaciens strain harboring ß-glucuronidase and neomycin phosphotransferase ii genes. all the lines showed ß-glucuronidase activity by histological staining. dna analysis by southern blots of the kanamycin resistant embryogenic lines and of a plant regenerated from one of them confirmed the integration of the t-dn ... | 1993 | 24196848 |
| diversity of sporadic symbionts and nonsymbiotic endophytic bacteria isolated from nodules of woody, shrub, and food legumes in ethiopia. | fifty-five bacterial isolates were obtained from surface-sterilized nodules of woody and shrub legumes growing in ethiopia: crotalaria spp., indigofera spp., and erythrina brucei, and the food legumes soybean and common bean. based on partial 16s rrna gene sequence analysis, the majority of the isolates were identified as gram-negative bacteria belonging to the genera achromobacter, agrobacterium, burkholderia, cronobacter, enterobacter, mesorhizobium, novosphingobium, pantoea, pseudomonas, rahn ... | 2013 | 24196581 |
| a simple procedure for the expression of genes in transgenic soybean callus tissue. | cotyledons from germinating seeds of the soybean cultivar peking were inoculated with virulent agrobacterium tumefaciens strain a281:pza-7 which carries a wild type ti plasmid ptibo542 and a disarmed ti plasmid (a binary vector)pza-7 which contains the βglucuronidase (uida) and neomycin phosphotransferase (nptii) genes. tumors were produced on all inoculated explants and 82% of these tumor lines were cotransformed by the nptii gene from the binary vector pza-7 as shown by pcr analysis (18 of 22 ... | 1994 | 24196243 |
| agrobacterium-mediated transformation of apple (malus x domestica borkh.): an assessment of factors affecting gene transfer efficiency during early transformation steps. | the factors influencing transfer of an intron - containing β-glucuronidase gene to apple leaf explants were studied during early steps of an agrobacterium tumefaciens-mediated transformation procedure. the gene transfer process was evaluated by counting the number of β-glucuronidase expressing leaf zones immediately after cocultivation, as well as by counting the number of β-glucuronidase expressing calli developing on the explants after 6 weeks of postcultivation in the presence of 50 mg/l kana ... | 1994 | 24196227 |
| agrobacterium tumefaciens mediated gene transfer in peanut (arachis hypogaea l.). | transgenic peanut plants were produced using agrobacterium mediated gene transfer. primary leaf explants of peanut were co-cultivated with agrobacterium tumefaciens lba 4404 harbouring the binary plasmid pbi 121 (conferring β-glucuronidase activity and resistance to kanamycin) and cultured on regeneration medium supplemented with kanamycin to select putatively transformed shoots. they were rooted and plants were transferred to soil. stable integration and expression of the transgenes were confir ... | 1994 | 24196226 |
| strain and cultivar specificity in the agrobacterium-chickpea interaction. | the susceptibility of four genotypes of chickpea to four wild strains of agrobacterium tumefaciens was evaluated. successful transformation was dependent on specific bacterial strain-plant cultivar interactions. agropine strain a281 was the most effective for tumor induction. tumors displayed hormone autonomous growth, were opine positive and contained dna that was homologous to the t-dna of the inciting strain. | 1994 | 24196221 |
| relationship between transcript production and virus resistance in transgenic tobacco expressing the potato leafroll virus coat protein gene. | the coat protein (cp) gene of potato leafroll luteovims (plrv) was inserted into tobacco (nicotiana tabacum) using disarmed agrobacterium tumefaciens (lba4404) containing a binary expression vector. plrv cp gene transcript was detected in transgenic plants but its abundance differed between transformed lines. cp was not detected in virus-free transgenic plants. the segregation of kanamycin resistance in s1 seedling progenies (obtained by selfing transformed plants) indicated that multiple (up to ... | 1993 | 24196184 |
| cysteine desulphurase-encoding gene sufs2 is required for the repressor function of rira and oxidative resistance in agrobacterium tumefaciens. | the agrobacterium tumefaciens genome contains a cluster of genes that are predicted to encode fe-s cluster assembly proteins, and this cluster is known as the sufs2bcds1xa operon. sufs2 is the first gene in the operon, and it was inactivated to determine its physiological function. the sufs2 mutant exhibited a small colony phenotype, grew slower than the wild-type strain and was more sensitive to various oxidants including peroxide, organic hydroperoxide and superoxide. the sufs2 gene was negati ... | 2014 | 24194559 |
| asymmetric somatic hybrid plants between an interspecific lycopersicon hybrid and solanum melongena. | asymmetric somatic hybrid plants were obtained by a modified peg/dmso fusion procedure between protoplasts derived from suspension cells of an interspecific tomato hybrid, lycopersicon esculentum x l. pennellii, and mesophyll protoplasts of solanum melongena, eggplant. the tomato hybrid was previously transformed with agrobacterium tumefaciens and contained the kanamycin-resistance marker gene. prior to fusion, the donor protoplasts of the tomato hybrid were gamma irradiated at 9.0 krad. thus, n ... | 1995 | 24194315 |
| agrobacterium-mediated transformation and regeneration of fertile transgenic plants of chinese cabbage (brassica campestris ssp. pekinensis cv. 'spring flavor'). | a procedure for the regeneration of fertile transgenic chinese cabbage (brassica campestris ssp. pekinensis cv. 'spring flavor') is presented in this report. the protocol is based on infection of cotyledon explants of 5-d-old seedlings with an agrobacterium tumefaciens strain lba4404 carrying a disarmed binary vector ptok/bks-1. the t-dna region of this binary vector contains the nopaline synthase/neomycin phosphotransferase ii (nptii) chimeric gene for kanamycin resistance and the cauliflower m ... | 1995 | 24194308 |
| agrobacterium-mediated transformation of sweet orange and regeneration of transgenic plants. | transgenic sweet orange (citrus sinensis l. osbeck) plants have been obtained by agrobacterium tumefaciens-mediated gene transfer. an hypervirulent a. tumefaciens strain harboring a binary vector that contains the chimeric neomycin phosphotransferase ii (npt ii) and ß-glucuronidase (gus) genes was cocultivated with stem segments from in vivo grown seedlings. shoots regenerated under kanamycin selection were harvested from the stem segments within 12 weeks. shoot basal portions were assayed for g ... | 1995 | 24194307 |
| stable expression of the gus reporter gene in chrysanthemum depends on binary plasmid t-dna. | three chrysanthemum (dendranthema grandiflora) cultivars were cocultivated with 2 agrobacterium tumefaciens strains in combination with 4 pbin19 derived binary plasmids, all carrying the nosnptll selection gene and 35sgus(intron) reporter gene. all binary plasmids transferred dna to chrysanthemum explants but only pmog410 gave good stable expression of gus. this plasmid differs from the other plasmids in 2 aspects: 1) it carries a restored nptll gene and 2) the selection gene is positioned at th ... | 1994 | 24194229 |
| introduction and expression of an insect proteinase inhibitor in alfalfa medicago sativa l. | as one approach to alleviating the need for insecticide spraying, our objective is to express protein insecticides in transgenic alfalfa. to initiate these studies, a cdna encoding the protease inhibitor (pi) anti-elastase from manduca sexta was placed under the control of the camv 35s promoter, inserted into pan 70, and transferred into leaf and petiole sections of alfalfa (medicago sativa l.) using agrobacterium tumefaciens mediated gene transfer. transformation rates were 10% of all explants ... | 1994 | 24194223 |
| transgenic plums (prunus domestica l.) express the plum pox virus coat protein gene. | plum hypocotyl slices were transformed with the coat protein (cp) gene of plum pox virus (ppv-cp) following cocultivation with agrobacterium tumefaciens containing the plasmid pga482gg/ppvcp-33. this binary vector carries the ppv-cp gene construct, as well as the chimeric neomycin phosphotransferase and β-glucuronidase genes. integration and expression of the transferred genes into regenerated plum plants was verified through kan resistance, gus assays, and pcr amplification of the ppv-cp gene. ... | 1994 | 24194220 |
| a thin layer chromatographic technique for detecting inducers of agrobacterium virulence genes in corn, wheat and rye. | a method is described for detecting plant metabolites capable of inducing the virulence genes of agrobacterium tumefaciens. the method uses a. tumefaciens containing a plasmid with an inducible virulence gene fused to a galactosidase gene (vire::lacz). thin layer chromatography plates are overlayed with agar containing the indicator bacterium and a chromogenic galactoside (x-gal). virulence gene inducing plant metabolites induce galactosidase which releases an aglycone readily oxidized by air to ... | 1994 | 24194126 |
| regeneration of plants from leaf explants of micropropagated clonal eucalyptus grandis. | callus production along with caulogenesis was obtained from leaf explants of micropropagated clonal eucalyptus grandis after six to twelve weeks of culture. out of eight clones tested, six were amenable to shoot production using simple media containing naphthaleneacetic acid and either 6-benzyladenine or zeatin. differences in growth regulator requirements for organogenesis were observed between different clones. these shoots were then elongated on a medium containing gibberellic acid and rooted ... | 1994 | 24194029 |
| tissue-specific and stress-enhancing expression of the tr promoter for mannopine synthase in transgenic medicinal plants. | transgenic tobacco (nicotiana tabacum l.), licorice (glycyrrhiza uralensis fisher) and foxglove (digitalis purpurea l.) were obtained with binary vector systems based on a disarmed agrobacterium tumefaciens and on a virulent a. rhizogenes. the chimeric neomycin phosphotransferase ii (npt-ii) gene (kan) and the ß-glucuronidase (gus) gene (uida) were under the control of the tr1' and 2' promoters, respectively, on a binary vector, pgsgluc1. tissue-specific expression of the chimeric tr2' -uida gen ... | 1991 | 24193927 |
| field performance of derived generations of transgenic tobacco. | two inbred cultivars of nicotiana tabacum (tobacco), 'samsun' and 'xanthi', were transformed with the plasmid pbi 121 using bin 19 in agrobacterium tumefaciens. the plasmid carries the nptii gene conferring kanamycin resistance and the uida gene encoding β-glucuronidase (gus). progeny carrying the genes in the homozygous condition were identified and selfed over several generations. one line homozygous for the introduced genes and one untransformed control from each cultivar were then selected a ... | 1993 | 24193884 |
| protection against virus infection in tobacco plants expressing the coat protein of grapevine fanleaf nepovirus. | grapevine fanleaf nepovirus (gflv) is responsible for the economically significant "court-noué" disease in vineyards. its genome is made up of two single-stranded rna molecules (rna1 and rna2) which direct the synthesis of polyproteins p1 and p2 respectively. a chimeric coat protein gene derived from the c-terminal part of p2 was constructed and subsequently introduced into a binary transformation vector. transgenic nicotiana benthamiana plants expressing the coat protein under the control of th ... | 1994 | 24193837 |
| genetic transformation and plant regeneration of watermelon using agrobacterium tumefaciens. | adventitious shoots formed on the proximal cut edges of different cotyledonary explants of watermelon [citrullus lanatus (thunb.) matsum. & nakai; cvs. sweet gem and gold medal] cultured on murashige and skoog's (ms) medium with 1 mgl(-1) 6-benzyladenine (ba). light (16-h photoperiod, about 7 wm(-2) cool-white fluorescent lamps) was essential for shoot formation. to obtain transformed plants, cotyledonary explants of 'sweet gem' were cocultured with agrobacterium tumefaciens lba4404, a disarmed ... | 1994 | 24193834 |
| effect of plant genotype on the transformation of cultivated alfalfa (medicago sativa) by agrobacterium tumefaciens. | the trait for somatic embryogenesis is being introduced sexually into alfalfa (medicago sativa) breeding populations to facilitate genetic transformation of this crop. cocultivation experiments were conducted with an agronomically-improved embryogenic clone from one such population as well as with two other embryogenic clones, one of which was the source of the embryogenic trait in the breeding populations. transgenic plants were produced from the agronomically-improved clone whereas none were p ... | 1994 | 24193831 |
| high efficiency agrobacterium-mediated gene transfer to flax. | using an agrobacterium tumefaciens binary vector (pal4404, pbi131), we have demonstrated the transfer of the β-glucuronidase gene into the flax (linum usitatissimum l.) cultivar glenelg after selection for kanamycin resistance. the transformed lines were obtained by inoculation and subsequent regeneration of hypocotyl segments. the callus that formed on the cut surfaces of the hypocotyl segments was isolated three weeks after infection and was subsequently subcultured to yield shoots. this proce ... | 1994 | 24193765 |
| genetic transformation of populus nigra by agrobacterium tumefaciens. | two clones of populus nigra l. were tested in vivo and in vitro for their susceptibility to three different agrobacterium tumefaciens wild-type strains evaluating number and size of resulting calluses. strain c58 proved to be the most virulent.various parameters affecting agrobacterium-mediated transformation of p. nigra clones were further analyzed using ß-glucuronidase gene transient expression. the clone jean pourtet proved to be more susceptible than the clone san giorgio. a. tumefaciens str ... | 1994 | 24193760 |
| the reversible inhibition of pollen germination of nicotiana tabacum l.: an entry into a transformation system. | the hydrodynamics of mature pollen rehydration in nicotiana tabacum was used to study reversible inhibition of pollen germination in vitro. tobacco pollen was incubated for various times in media containing calcium, potassium and magnesium salts, boric acid, and exhibiting different osmotic pressures as a function of sucrose concentration. total inhibition of germination with complete viability preservation was achieved for 56 h by keeping the grains in medium with 80% sucrose, since typical per ... | 1994 | 24193658 |
| biolistic transformation of tobacco and maize suspension cells using bacterial cells as microprojectiles. | we have used both escherichia coli cells and agrobacterium tumefaciens cells as microprojectiles to deliver dna into suspension-cultured tobacco (nicotiana tabacum l. line nt1) cells using a helium powered biolistic device. in addition, e. coli cells were used as microprojectiles for the transformation of suspension-cultured maize (zea mays cv. black mexican sweet) cells. pretreating the bacterial cells with phenol at a concentration of 1.0%, and combining the bacterial cells with tungsten parti ... | 1994 | 24193654 |
| a reporter gene under the control of tms or aux promoters is differentially expressed in tobacco and barley protoplasts. | agrobacterium tumefaciens and some agrobacterium rhizogenes strains possess auxin biosynthesis genes (tms and aux genes respectively), responsible for a de novo auxin biosynthetic pathway in transformed plant cells. a comparison is presented of the potential expression of these genes in a monocotyledonous (barley) and a dicotyledonous plant (tobacco). the promoters of the genes were translationally fused to the β-glucuronidase reporter gene and analysed in transient expression experiments. the t ... | 1994 | 24193642 |
| agrobacterium-mediated transformation of commercial melon (cucumis melo l., cv. amarillo oro). | cotyledon explants of muskmelon (cucumis melo l., cv. amarillo oro) seedlings were co-cultivated with disarmed agrobacterium tumefaciens strain lba4404 that contained the binary vector plasmid pbi121.1. the t-dna region of this binary vector contains the nopaline synthase/neomycin phosphotransferase ii (nptii) chimeric gene for kanamycin resistance and the cauliflower mosaic virus 35s/β-glucuronidase (gus) chimeric gene. after infection, the cotyledon pieces were placed in induction medium conta ... | 1994 | 24193640 |
| agrobacterium-mediated transformation of white mustard (sinapis alba l.) and regeneration of transgenic plants. | a procedure for the regeneration of fertile transgenic white mustard (sinapis alba l.) is presented. the protocol is based on infection of stem explants of 7-9 day old plants with an agrobacterium tumefaciens strain harboring a disarmed binary vector with chimeric genes encoding neomycin phosphotransferase and β-glucuronidase. shoots are regenerated from callus-forming explants within 3-4 weeks. under selection, 10% of the explants with transgenic embryonic callus develop into fertile transgenic ... | 1994 | 24193637 |
| regulatory sequences involved in the peroxidase gene expression in arabidopsis thaliana. | organ-specific expression of two peroxidase genes (prxca and prxea) from arabidopsis thaliana was studied. the prxca gene showed non-specific expression with relatively high levels of mrna accumulation in the roots, stems and leaves of a. thaliana. the prxea gene, on the other hand, accumulated high levels of mrna only in roots. promoter fragments from each gene were fused to the coding region of β-glucuronidase (gusa) reporter gene introduced into tobacco. promoter/gusa contructs were transferr ... | 1994 | 24193636 |
| transformation of passionfruit (passiflora edulis fv flavicarpa degener.) using agrobacterium tumefaciens. | leaf and stem explants of passionfruit (passiflora eadulis fv flavicarpa) were co-cultivated with a disarmed strain of agrobacterium tunefaciens harbouring the co-integrate vector pmon200. four plants of passionfruit were regenerated from leaf explants on agar-solidified murashige and skoog (1962) based medium containing 4.43 μm 6-benzyl-aminopurine and supplemented with 86 μm kanamycin sulphate. the four plants were rooted by transfer to ms based medium with 14.7 μm 3-indolebutyric acid and 2.6 ... | 1994 | 24193523 |
| transformation of potato (solanum tuberosum) cv. mantiqueira using agrobacterium tumefaciens and evaluation of herbicide resistance. | in search of establishing a system for genetic transformation of brazilian potato cultivars, agrobacterium tumefaciens carrying the plasmid pgv1040, was used to transform leaf discs of three cultivars of local importance, i.e., aracy, baronesa and mantiqueira. this plasmid contains marker genes for resistance to kanamycin and phosphinothricin plus the gene for the enzyme β-glucuronidase. a two step regeneration/selection procedure produced shoots of potato cultivar mantiqueira with in vitro resi ... | 1994 | 24193516 |
| nuclear genomic composition of asymmetric fusion products between irradiated transgenic solanum brevidens and s. tuberosum: limited elimination of donor chromosomes and polyploidization of the recipient genome. | the production of asymmetric somatic hybrid calli after fusion between gamma-irradiated protoplasts from transgenic solanum brevidens and protoplasts from s. tuberosum are reported. transgenic (kanamycin-resistant, gus-positive) s. brevidens plants and hairy root clones were obtained after transformation with agrobacterium tumefaciens lba 1060 (pri1855) (pbi121) and lba 4404 (pral4404) (pbi121), and a. rhizogenes lba 9402 (pri1855) (pbi121), respectively. leaf protoplasts isolated from the trans ... | 1993 | 24193466 |
| production of virus resistant and insect tolerant transgenic tobacco plants. | the cucumber mosaic virus coat protein (cmv-cp) gene and a modified bacillus thuringiensis δ-endotoxin (bt toxin) gene were cloned into plant expression vector pe3. tobacco (nicotiana tabacum cv. g28) leaf discs were transformed with agrobacterium tumefaciens a12 carrying recombinant pe14. transgenic r0 and r1 tobacco plants expressing cmv-cp and bt toxin genes were protected from cmv infection as well as feeding damage of manduca sexta (tobacco hornworm) larvae. these results demonstrate that i ... | 1994 | 24192882 |
| agrobacterium-mediated transformation of the desiccation-tolerant plant craterostigma plantagineum. | an efficient procedure for agrobacterium tumefaciens- mediated transformation of the desiccation-tolerant plant craterostigma plantagineum has been developed. leaf explants were inoculated with a. tumefaciens strain gv3101 carrying the gene for kanamycin- or hygromycin-resistance and the ßglucuronidase reporter gene. parameters which affected the transformation efficiency were the age of the explant, the degree of wounding and the presence of an antioxidant in the medium. under optimal condition ... | 1994 | 24192874 |