Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| herpes simplex virus type 1 ul51 protein is involved in maturation and egress of virus particles. | the ul51 gene of herpes simplex virus type 1 (hsv-1) encodes a phosphoprotein whose homologs are conserved throughout the herpes virus family. recently, we reported that ul51 protein colocalizes with golgi marker proteins in transfected cells and that targeting of ul51 protein to the golgi apparatus depends on palmitoylation of its n-terminal cysteine at position 9 (n. nozawa, t. daikoku, t. koshizuka, y. yamauchi, t. yoshikawa, and y. nishiyama, j. virol. 77:3204-3216, 2003). however, its role ... | 2005 | 15890934 |
| the carboxyl-terminal domain of rna polymerase ii is phosphorylated by a complex containing cdk9 and infected-cell protein 22 of herpes simplex virus 1. | the infected-cell protein 22 (icp22), a regulatory protein encoded by the alpha22 gene of herpes simplex virus 1, is required for the optimal expression of a set of late viral proteins that includes the products of the u(s)11, u(l)38, and u(l)41 genes. icp22 has two activities. thus, icp22 and the u(l)13 protein kinase mediate the activation of cdc2 and degradation of its partners, cyclins a and b. cdc2 and its new partner, the dna polymerase accessory factor (u(l)42), bind topoisomerase iialpha ... | 2005 | 15890914 |
| antiviral evaluation of plants from brazilian atlantic tropical forest. | the antiviral activity of six medicinal plants from brazilian atlantic tropical forest was investigated against two viruses: herpes simplex virus type 1 (hsv-1) and poliovirus type 2 (pv-2). cuphea carthagenensis and tillandsia usneoides extracts showed the best antiherpes activity. t. usneoides dichloromethane, ethyl acetate and n-butanol extracts, and lippia alba n-butanol extract showed inhibition of hsv-1, strain 29r/acyclovir resistant. in addition, only l. alba ethyl acetate extract showed ... | 2005 | 15890472 |
| [reactivation of persistent herpes virus infection as a factor of endogenous uveitis in children]. | two hundred and fifty-five children with endogenous uveitis, aged 2 months to 15 years, were examined. 73-93% of children were chronically infected by different viruses of the human herpes group; mycoplasmal past-infection was detected in only 13% of them and chlamydia past-infection _ in 3.7% of them. herpes simplex virus of type 1 reactivated reliably more often versus other types of herpesviridae. reactivation of cytomegalovirus infection prevailed in mothers who gave birth to children with i ... | 2005 | 15881151 |
| the role of viral and host genes in corneal infection with herpes simplex virus type 1. | herpes simplex virus infection of the eye is the leading cause of blindness due to infection in the us despite the availability of several antiviral drugs. studies with animal models have shown that three factors, innate host resistance, the host adaptive immune response, and the strain of virus interact to determine whether an infection is asymptomatic or proceeds to the development of blinding keratitis (hsk). of these, the role of adaptive immunity has received the most attention. this work h ... | 2005 | 15862167 |
| lethal encephalitis in myeloid differentiation factor 88-deficient mice infected with herpes simplex virus 1. | herpes simplex virus 1 (hsv-1), a large dna virus from the herpesviridae family, is the major cause of sporadic lethal encephalitis and blindness in humans. recent studies have shown the importance of toll-like receptors (tlrs) in the immune response to hsv-1 infection. myeloid differentiation factor 88 (myd88) is a critical adaptor protein that is downstream to mediated tlr activation and is essential for the production of inflammatory cytokines. here, we studied the relationship between myd88 ... | 2005 | 15855642 |
| dynamics of the antiviral activity of n-methanocarbathymidine against herpes simplex virus type 1 in cell culture. | n-methanocarbathymidine [(n)-mct], a thymidine analogue, exhibits potent activity in cell culture against herpes simplex virus1 (hsv-1). (n)-mct showed higher antiviral activity than ganciclovir (gcv). continuous treatment of vero cells with (n)-mct immediately or 10 h post-infection (p.i.) fully prevented the development of viral infection. however, when infected cells were treated with (n)-mct at 12 h p.i., there was only a partial inhibition (ca. 50%). additionally, continuous treatment of in ... | 2005 | 15848299 |
| detection and typing of herpes simplex dna in genital swabs by real-time polymerase chain reaction. | the lightcycler polymerase chain reaction (pcr) is a sensitive assay for the detection of herpes simplex virus (hsv) dna in muco-cutaneous swabs. software-based analysis of the probe melting temperature (tm) can be used to discriminate between hsv types (hsv-1 and hsv-2). among 76 hsv dna positive genital swabs, atypical tms were observed in 14 (18%). the 14 samples were all typed as hsv-2 by sequence alignment. in 4/14 samples, the atypical tm was associated with sequence variation at the probe ... | 2005 | 15847938 |
| study of the viral infections and cytokines associated with recurrent aphthous ulceration. | mouth ulcers are one of the most common oral complaints. however, the association between oral ulceration and viruses and cytokines is uncertain. we detected the presence of human papilloma virus (hpv), cytomegalovirus (cmv), epstein-barr virus (ebv), herpes simplex virus (hsv)-1, hsv-2 and human herpesvirus (hhv)-8 dna in oral tissues by polymerase chain reaction (pcr) and southern hybridization techniques, and quantified the serum levels of cytokines including interleukin (il)-2, il-4, granulo ... | 2005 | 15840465 |
| dna polymerase gene locus of cercopithecine herpesvirus 1 is a suitable target for specific and rapid identification of viral infection by pcr technology. | the family herpesviridae comprises at least 100 herpesviruses. numerous human and animal pathogenic herpesviruses have been identified so far, including cercopithecine herpesvirus 1 (cehv-1). this virus is a member of the subfamily alphaherpesvirinae and is the most hazardous herpesvirus to man. cehv-1 is also known as b-virus or monkey b virus and as herpesvirus simiae. in order to gain more genetic information, the viral dna polymerase (dpol) gene was identified using polymerase chain reaction ... | 2005 | 15830148 |
| cd8+-dependent cns demyelination following ocular infection of mice with a recombinant hsv-1 expressing murine il-2. | demyelinating diseases comprise a spectrum of immunopathologic syndromes in which myelin, the fatty covering of nerve cell fibers in the brain and spinal cord, is destroyed. in this study, we have shown for the first time that ocular infection of balb/c mice with a recombinant herpes simplex virus type 1 (hsv-1) expressing il-2 (hsv-il-2) results in cns demyelination as determined by light microscopy and em. the demyelinated lesions involve periventricular white matter, brain stem, and spinal co ... | 2005 | 15817260 |
| primary cutaneous plasmacytoma: a role for a triggering stimulus? | primary cutaneous plasmacytoma is a rare type of cutaneous b-cell lymphoma, characterized by clonal proliferation of plasma cells, that primarily develops in the skin. five cases have been described to date in which a local triggering stimulus may be involved in development of this skin tumour. we describe the case of a primary cutaneous plasmacytoma localized to the lower lip. this site had been affected for 15 years with recurrent herpes simplex virus-1 infection. neoplastic plasma cells were ... | 2005 | 15807675 |
| a systematic review of the epidemiology and interaction of herpes simplex virus types 1 and 2. | to explore epidemiological evidence about the interaction of herpes simplex virus (hsv) 1 and hsv-2 infections. | 2005 | 15800084 |
| a branched, synthetic oligopeptide corresponding to a region of glycoprotein g of hsv-1 reacts sensitively and specifically with hsv-1 antibodies in an elisa. | herpes simplex viruses types 1 and 2 (hsv-1 and hsv-2), which are common worldwide, are so similar that antibodies directed against one serotype may crossreact with antigens from the other one. methods for specific detection of antibodies against hsv-1 or hsv-2 are based upon the antigenicities of glycoproteins g. however, due to the cost, the available commercial methods may not readily be used in developing countries. a different enzyme-linked immunosorbent assay (elisa) method, based upon a s ... | 2005 | 15794983 |
| cytokine profiles and inflammatory cells during hsv-1-induced acute retinal necrosis. | to investigate infiltrating cells, cytokines, and kinetics of cytokine expression during acute retinal necrosis (arn) in the uninoculated eye after inoculation of herpes simplex virus (hsv)-1 into the anterior chamber of one eye of balb/c mice. | 2005 | 15790902 |
| mathematical analysis demonstrates that interferons-beta and -gamma interact in a multiplicative manner to disrupt herpes simplex virus replication. | several studies suggest that the innate interferons (ifns), ifn-alpha and ifn-beta, can act in concert with ifn-gamma to synergistically inhibit the replication of cytomegalovirus and herpes simplex virus type 1 (hsv-1). the significance of this observation is not yet agreed upon in large part because the nature and magnitude of the interaction between ifn-alpha/beta and ifn-gamma is not well defined. in the current study, we resolve this issue by demonstrating three points. first, the hyperboli ... | 2005 | 15784277 |
| association of human endogenous retroviruses with multiple sclerosis and possible interactions with herpes viruses. | the hypothesis that human endogenous retroviruses (hervs) play a role in autoimmune diseases is subject to increasing attention. hervs represent both putative susceptibility genes and putative pathogenic viruses in the immune-mediated neurological disease multiple sclerosis (ms). gammaretroviral herv sequences are found in reverse transcriptase-positive virions produced by cultured mononuclear cells from ms patients, and they have been isolated from ms samples of plasma, serum and csf, and chara ... | 2005 | 15782388 |
| [molecular genetic analysis of thimidine kinase from herpes simplex virus type 1]. | 2005 | 15773560 | |
| herpes simplex virus targeting to the egf receptor by a gd-specific soluble bridging molecule. | herpes simplex virus 1 (hsv-1) enters cells via initial binding of envelope glycoproteins (g) c and b to cell-surface glycosaminoglycans (gags) and subsequent membrane fusion involving envelope gd, gb, and gh/gl. current insights suggest that the fusion process is initiated by interaction of gd with a cognate cellular receptor, such as the widely distributed cell adhesion molecule nectin-1. to redirect the tropism of hsv-1, we have generated a soluble adapter protein (p-v528lh) comprising the gd ... | 2005 | 15771964 |
| avian adenovirus vector celo-tk displays anticancer activity in human cancer cells and suppresses established murine melanoma tumors. | avian adenovirus celo is a novel adenovirus vector system with the advantages of efficient production, high virion stability, and the absence of crossreactivity with ad5-neutralizing antibodies. in this study, we evaluated the anticancer efficacy of a celo vector encoding the herpes simplex virus type 1 thymidine kinase, a prodrug-activating therapeutic gene. vectors carrying the gene for hsv-tk or egfp under the control of the hcmv promoter in place of the "nonessential" region of the celo geno ... | 2005 | 15761475 |
| a dna recombination-based approach to eliminate papillomavirus infection. | at present, no treatments exist that effectively target and eliminate papillomaviruses (pvs) from infected cells or prevent its replication. we are employing a strategy to prevent virus replication in pv-infected cells through the conditional expression of the herpes simplex virus type 1 thymidine kinase (tk) gene. expression of tk in this system is expected to be triggered by a homologous recombination event between the endogenous pv genome and a nonexpressing tk gene cassette. recombination be ... | 2005 | 15756291 |
| [herpesvirus infection in patients with chronic glomerulonephritis]. | virological examinations of blood, urine and saliva in 75 patients with chronic glomerulonephritis (cg) revealed, in 95% of them, herpes-virus infections caused by herpes simplex virus, type 1 (34.4%), herpes simplex virus, type 2 (2.6%) and cytomegalovirus (12%) or mixed infections (46%). the infection rate in the control group of children without renal pathology was reliably lower. a majority of cg patients (94%) had a diagnostically significant level of antiherpetic antibodies, class igg, whi ... | 2005 | 15747870 |
| orientation determination by wavelets matching for 3d reconstruction of very noisy electron microscopic virus images. | in order to perform a 3d reconstruction of electron microscopic images of viruses, it is necessary to determine the orientation (euler angels) of the 2d projections of the virus. the projections containing high resolution information are usually very noisy. this paper proposes a new method, based on weighted-projection matching in wavelet space for virus orientation determination. in order to speed the retrieval of the best match between projections from a model and real virus particle, a hierar ... | 2005 | 15743519 |
| evaluation of some pyrazoloquinolines as inhibitors of herpes simplex virus type 1 replication. | three structurally related aminopyrazoloquinoline derivatives were evaluated for their antiviral activity against herpes simplex virus type 1. these compounds were examined for their in vitro antiviral activity by two different bioassays, namely; crystal violet staining and tetrazolium dye (mts) measurement. the antiviral role of these compounds was confirmed by enumerating the infectious particles with plaque assay. the acute toxicity values of the biologically active compounds were determined ... | 2005 | 15736285 |
| identification of an essential domain in the herpes simplex virus 1 ul34 protein that is necessary and sufficient to interact with ul31 protein. | previous results have indicated that the herpes simplex virus 1 ul31 and ul34 proteins interact and form a complex at the inner nuclear membranes of infected cells, where both play important roles in the envelopment of nucleocapsids at the inner nuclear membrane. in the work described here, mapping studies using glutathione s-transferase pull-down assays indicated that amino acids 137 to 181 of the ul34 protein are sufficient to mediate an interaction with the ul31 protein. a recombinant virus ( ... | 2005 | 15731273 |
| fluorosubstitution and 7-alkylation as prospective modifications of biologically active 6-aryl derivatives of tricyclic acyclovir and ganciclovir analogues. | a series of fluorine containing tricyclic analogues of acyclovir (acv, 1) and ganciclovir (gcv, 2) were synthesized and evaluated for their activity against herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) and cytostatic activity against hsv-1 thymidine kinase (tk) gene-transduced human osteosarcoma tumour cells. it was found that fluorine substitution reduced the antiviral activity, but most of the new compounds were pronounced cytostatic agents with potency and selectivity similar to tho ... | 2005 | 15727862 |
| liver-directed therapies in colorectal cancer. | the liver is the most common site of metastatic colorectal cancer (crc) and the status of this organ is an important determinant of overall survival in patients with advanced disease. complete resection of hepatic crc metastases can provide a long-term cure for some patients, but the majority of liver metastases are not amenable to such surgery. furthermore, most patients after curative resection ultimately suffer from recurrence, and the majority of such failures occur in the liver. various abl ... | 2005 | 15726510 |
| regulation of ctl responses to mhc-restricted class i peptide of the gp70 tumour antigen by splenic parenchymal cd4+ t cells in mice failing immunotherapy with disc-mgm-csf. | direct intratumour injection of the disabled infectious single-cycle-herpes simplex virus-encoding murine granulocyte/macrophage colony-stimulating factor (disc-hsv-mgm-csf) into established colon carcinoma ct26 tumours induced complete tumour rejection in up to 70% of treated animals (regressors), while the remaining mice developed progressive tumours (progressors). this murine balb/c model was used to dissect the cellular mechanisms involved in tumour regression or progression following immuno ... | 2005 | 15723299 |
| multimutated herpes simplex virus g207 is a potent inhibitor of angiogenesis. | the mode of the antitumoral activity of multimutated oncolytic herpes simplex virus type 1 g207 has not been fully elucidated yet. because the antitumoral activity of many drugs involves the inhibition of tumor blood vessel formation, we determined if g207 had an influence on angiogenesis. monolayers of human umbilical vein endothelial cells and human dermal microvascular endothelial cells, but not human dermal fibroblasts, bronchial epithelial cells, and retinal glial cells, were highly sensiti ... | 2004 | 15720798 |
| extensive primary cutaneous herpes simplex virus type 1 infection in an infant following acute rotavirus gastroenteritis. | 2004 | 15717179 | |
| possible mechanism underlying the antiherpetic activity of a proteoglycan isolated from the mycelia of ganoderma lucidum in vitro. | glpg (ganoderma lucidum proteoglycan) was a bioactive fraction obtained by the liquid fermentation of the mycelia of ganoderma lucidum, etoh precipitation, and deae-cellulose column chromatography.glpg was a proteoglycan with a carbohydrate: protein ratio of 10.4: 1. its antiviral activities against herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) were investigated using a cytopathic inhibition assay. glpg inhibited cell death in a dose-dependent manner in hsv-infected cells. in addition, ... | 2005 | 15715944 |
| phenolics with antiviral activity from millettia erythrocalyx and artocarpus lakoocha. | from the leaves of millettia erythrocalyx, a new flavone named 3',5'-dimethoxy-[2",3": 7,8]-furanoflavone and three known compounds were isolated. assays for anti-herpes simplex virus activity (hsv-1 and hsv-2) were performed on 24 phenolic compounds obtained from m. erythrocalyx and artocarpus lakoocha. it was found that the flavones ovalifolin, pongol methyl ether and millettocalyxin a, and the stilbene oxyresveratrol possessed moderate activity against both types of hsv. in addition, oxyresve ... | 2005 | 15715263 |
| vesiculobullous lesion on the right fifth toe. | 2005 | 15714680 | |
| the ectodomain of herpes simplex virus glycoprotein h contains a membrane alpha-helix with attributes of an internal fusion peptide, positionally conserved in the herpesviridae family. | human herpesviruses enter cells by fusion with target membranes, a process that requires three conserved glycoproteins: gb, gh, and gl. how these glycoproteins execute fusion is unknown. neural network bioinformatics predicted a membrane alpha-helix contained within the ectodomain of herpes simplex virus (hsv) gh, positionally conserved in the gh of all examined herpesviruses. evidence that it has attributes of an internal fusion peptide rests on the following lines of evidence. (i) the predicte ... | 2005 | 15709012 |
| bell's palsy and herpes simplex virus: fact or mystery? | in recent years, progress has been made in the understanding of bell's palsy, the most common form of acute facial weakness. herpes simplex virus (hsv) reactivation within the geniculate ganglion with subsequent inflammation and entrapment of the nerve at the meatal foramen has been proposed to be the pathogenetic mechanism. we challenged its accuracy by analyzing our own data on the presence of viral genomic dna of hsv-1 and 2, human herpes virus (hhv)-6a/b, as well as varizella zoster virus (v ... | 2005 | 15699730 |
| rapid diagnosis of herpes simplex virus infection by a loop-mediated isothermal amplification method. | primers for herpes simplex virus type 1 (hsv 1)-specific loop-mediated isothermal amplification (lamp) method amplified hsv-1 dna, while hsv-2-specific primers amplified only hsv-2 dna; no lamp products were produced by reactions performed with other viral dnas. the sensitivities of the hsv-1- and hsv-2-specific lamp methods, determined by agarose gel electrophoresis, reached 500 and 1,000 copies/tube, respectively. the turbidity assay, however, determined the sensitivity of the hsv-1- and hsv-2 ... | 2005 | 15695716 |
| role of natural killer t (nkt) cells lacking interleukin (il)-4 producing abilities on the cc-chemokine ligand 2-associated herpes simplex virus type 1 infection in human severe combined immunodeficiency (scid) mouse chimeras. | cc-chemokine ligand 2 (ccl2/monocyte chemoattractant protein 1) is known to have an important role on t helper type 2 (th2) cell generation and described to induce interleukin (il)-4 production by activated t cells. in the present study, an increase of ccl2 production in cultures of peripheral blood lymphocytes (pbl) from patients with severe thermal injuries was demonstrated. severe combined immunodeficiency (scid) mice reconstituted with pbl from healthy donors (pbl-scid chimeras) were resista ... | 2005 | 15683684 |
| human dendritic cells transduced with herpes simplex virus amplicons encoding human immunodeficiency virus type 1 (hiv-1) gp120 elicit adaptive immune responses from human cells engrafted into nod/scid mice and confer partial protection against hiv-1 challenge. | small-animal models are needed to test human immunodeficiency virus (hiv) vaccine efficacy following viral challenge. to this end, we examined hiv-1-specific immune responses following immunization of nonobese diabetic-severe combined immunodeficient mice that were repopulated with human peripheral blood lymphocytes (hu-pbl-nod/scid mice). autologous dendritic cells (dc) were transduced ex vivo with replication-defective, helper virus-free, herpes simplex virus type 1 (hsv-1) amplicons that expr ... | 2005 | 15681415 |
| an association of herpes simplex virus type 1 infection with type 2 diabetes. | 2005 | 15677810 | |
| the herpes simplex virus type 1 dna polymerase processivity factor, ul42, does not alter the catalytic activity of the ul9 origin-binding protein but facilitates its loading onto dna. | the herpes simplex virus type 1 ul42 dna polymerase processivity factor interacts physically with ul9 and enhances its ability to unwind short, partially duplex dna. in this report, atp hydrolysis during translocation of ul9 on single-stranded (ss) or partially duplex dna was examined in the presence and absence of ul42 to determine the effect of ul42 on the catalytic function of ul9. our studies reveal that a homodimer of ul9 is sufficient for dna translocation coupled to atp hydrolysis, and th ... | 2005 | 15673714 |
| new conformationally locked bicyclic n,o-nucleoside analogues of antiviral drugs. | in order to obtain rigidity within the sugar moiety of nucleosides, the bicyclic pyrimidine derivatives of n,o-isoxazolidines were designed and synthesized by using 1,3-dipolar cycloaddition of delta(1)-pyrrolidine-1-oxide and the appropriate vinyl-nucleobases. | 2005 | 15664810 |
| combination of mutated herpes simplex virus type 1 (g207 virus) with radiation for the treatment of squamous cell carcinoma of the head and neck. | g207 is an oncolytic herpes simplex virus (hsv) with deletions at both gamma134.5 loci and a lacz gene insertion inactivating the hsv ribonucleotide reductase gene. ionising radiation induces the growth arrest-inducible gene, gadd34, and ribonucleotide reductase. gadd34 is a protein that correlates with apoptosis following radiation and has homology with the g207 gamma134.5 gene. we hypothesised that the combination of radiotherapy with g207 may have a potentiating effect on viral replication an ... | 2005 | 15661558 |
| type-specific reactivity of anti-glycoprotein g antibodies from herpes simplex virus-infected individuals is maintained by single or dual type-specific residues. | glycoprotein g-1 (gg-1) of herpes simplex virus type 1 (hsv-1) and gg-2 of hsv-2 are the only known hsv proteins that induce type-specific human antibody responses. recently, it was shown that purified human anti-gg-1 and anti-gg-2 antibodies presented a type-specific reactivity to immunogenic stretches with high similarity between gg-1 and gg-2. in this study, the molecular basis for this type-specific recognition was investigated employing synthetic peptides covering the indicated regions, inc ... | 2005 | 15659743 |
| effective gene delivery to adult neurons by a modified form of electroporation. | non-viral methods of transfection of cdnas into adult neurons and other post-mitotic cells are generally very inefficient. however, the recent development of nucleofector technology developed by amaxa biosystems allows direct delivery of cdnas into the nucleus, enabling transfection of non-dividing cells. in this study, we describe a reliable method for culturing large numbers of retinal cells from adult rats and using nucleofection, we were able to transfect cdna-encoding gfp (jellyfish green f ... | 2005 | 15652627 |
| herpes simplex virus 1 infection activates the endoplasmic reticulum resident kinase perk and mediates eif-2alpha dephosphorylation by the gamma(1)34.5 protein. | the gamma(1)34.5 protein of herpes simplex virus (hsv) plays a crucial role in virus infection. although the double-stranded rna-dependent protein kinase (pkr) is activated during hsv infection, the gamma(1)34.5 protein inhibits the activity of pkr by mediating dephosphorylation of the translation initiation factor eif-2alpha. here we show that hsv infection also induces phosphorylation of an endoplasmic reticulum (er) resident kinase perk, a hallmark of er stress response. the virus-induced pho ... | 2005 | 15650164 |
| "suicide" gene therapy of breast cancer cells is only cytostatic in vitro but anti-tumoral in vivo on breast mcf7-ras tumor. | gene therapy with herpes simplex virus thymidine kinase gene (hsv-tk) is effective in various tumor models in vitro and in vivo. we compared the efficacy of the hsv-tk gene therapy in vitro and in vivo in mcf-7 and mcf7-ras cells which form tumor in athymic mice. after viral infection, cells were treated with gcv (ganciclovir) and live cells were counted. the in vitro treatment significantly inhibited cell growth but did not induce early and late apoptosis, measured, respectively, by annexin or ... | 2004 | 15646826 |
| antiviral effect of chinonin against herpes simplex virus. | in order to investigate the antiviral effect of chinonin against herpes simplex virus (hsv), the encephalitis model in mice and skin infection model in guinea pigs were established by hsv- i and hsv-ii infection respectively. acyclovir was used as the positive reference drug to evaluate the antiviral capacity of chinonin. chinonin showed an obvious therapeutic effect on encephalitis in mice at doses of 25 and 50 mg/kg. at both dosages, chinonin demonstrated stronger protection than acyclovir (1 ... | 2004 | 15641710 |
| a novel role for neutrophils as a source of t cell-recruiting chemokines ip-10 and mig during the dth response to hsv-1 antigen. | analogous to cd4+ t cells, neutrophils are essential participants in delayed-type hypersensitivity (dth) to herpes simplex virus type 1 antigen. however, what role they play in this cellular immune response is unclear. the recent recognition that neutrophils are potent producers of chemokines led us to hypothesize that they may help recruit cd4+ effector t cells. in the present study, we show that neutrophil depletion was accompanied by a marked decrease in the numbers of cd4+ and cxc receptor 3 ... | 2005 | 15629884 |
| involvement of three mucous membranes in herpes-induced recurrent erythema multiforme. | 2005 | 15627109 | |
| efficient and non-toxic gene transfer to cardiomyocytes using novel generation amplicon vectors derived from hsv-1. | the feasibility of gene transfer to myocardial tissue using viral vectors was investigated over the last few years. in this study we report gene transfer using a recently described improved of herpes simplex virus (hsv-1)-derived amplicon vectors and demonstrate that these vectors are a powerful and potentially very interesting tool for gene transfer into neonatal primary as well as in adult cardiac myocytes. | 2004 | 15623439 |
| glycine-amide is an active metabolite of the antiretroviral tripeptide glycyl-prolyl-glycine-amide. | the chemically modified tripeptide glycyl-prolyl-glycine-amide (gpg-nh(2)) inhibits replication of human immunodeficiency virus (hiv) type 1 (hiv-1) in vitro, probably by interfering with capsid formation. the aim of the present study was to determine whether the metabolites glycyl-proline (gp-oh), glycine (g-oh), prolyl-glycine-amide (pg-nh(2)), proline (p-oh), and glycine-amide (g-nh(2)) from proteolytic cleavage may inhibit the replication of hiv-1 in vitro. pg-nh(2) has previously been shown ... | 2005 | 15616273 |
| association of viral factors with non-familial breast cancer in taiwan by comparison with non-cancerous, fibroadenoma, and thyroid tumor tissues. | to study the etiologic factors of non-familial breast cancer, the polymerase chain reaction (pcr) and southern hybridization were used to detect six viruses including human papillomavirus (hpv), cytomegalovirus (cmv), epstein-barr virus (ebv), herpes simplex virus (hsv)-1, hsv-2, and human herpesvirus (hhv)-8 dna in 69 patients with breast cancer and 60 specimens from non-cancerous or other individuals with thyroid tumors or fibroadenoma (non-breast cancer controls). two specimens from patients ... | 2005 | 15602723 |
| identification of a region in the herpes simplex virus scaffolding protein required for interaction with the portal. | the herpes simplex virus type 1 capsid is a protective shell that acts as a container for the genetic material of the virus. after assembly of the capsid, the viral dna is translocated into the capsid interior through a channel formed by the portal. the portal is composed of a dodecamer of ul6 molecules which form a ring-like structure found at a single vertex within the icosahedron. formation of portal-containing capsids minimally requires the four structural proteins (vp5, vp19c, vp23, and ul6 ... | 2005 | 15596809 |
| herpes simplex virus-induced, death receptor-dependent apoptosis and regression of transplanted human cancers. | inoculation of a live attenuated herpes simplex virus (hsv) vector, betah1, into human u87mg glioblastoma cells transplanted into athymic nude mice induced complete regression of tumors. the infected cells underwent histochemically confirmed apoptosis without lymphocyte infiltration after expressing cd30, cd30 ligand (cd30l), tumor necrosis factor (tnf)-alpha, tnf receptor 1 (tnf-r1), fas, and fas ligand (fas-l) with activation of caspases 3 and 8. induction of the transcripts of these receptors ... | 2004 | 15596049 |
| expression of cellular proteins bcl-x(l), xiap and bax involved in apoptosis in cells infected with herpes simplex virus 1 and effect of pavine alkaloid (-)-thalimonine on virus-induced suppression of apoptosis. | the expression of three cellular proteins involved in the modulation of apoptosis, namely antiapoptotic bcl-x(l) and xiap and proapoptotic bax, was investigated in cells infected with herpes simplex virus 1 (hsv-1). to assess whether the regulation of apoptosis in virus-infected cells depends on strain specificity the wild-type (wt) strain victoria and the mutant r-100 resistant to acyclovir (acv) were used. in addition, the expression of bcl-x(l), xiap and bax was studied in cells infected with ... | 2004 | 15595215 |
| imaging progress of herpes simplex virus type 1 thymidine kinase suicide gene therapy in living subjects with positron emission tomography. | molecular imaging of a suicide transgene's expression will aid the development of efficient and precise targeting strategies, and imaging for cancer cell viability may assess therapeutic efficacy. we used the pet reporter probe, 9-(4-[18f]fluoro-3-(hydroxymethyl)butyl)guanine ([18f]fhbg) to monitor the expression of a mutant herpes simplex virus 1 thymidine kinase (hsv1-sr39tk) in c6 glioma tumors implanted subcutaneously in nude mice that were repetitively being treated with the pro-drug gancic ... | 2005 | 15592447 |
| ethnomedicinal uses of momordicacharantia (cucurbitaceae) in togo and relation to its phytochemistry and biological activity. | investigation of the traditional uses of momordica charantia (cucurbitaceae) in togo (west africa) showed that it is one of the most important local medicinal plants both for ritual and ethnomedical practices. there was a high degree of consensus (>50%) for use in the treatment of gastrointestinal and viral disease among 47 groups of village informants in the general population, while 19 traditional healers reported a larger and broader set of uses. the use by informants in gaur and kwa language ... | 2005 | 15588650 |
| [the synthesis and antiherpetic activity of acyclovir phosphonate esters]. | alkyl esters of acyclovir phosphite, alkoxycarbonylphosphonate, ethoxycarbonylmethylphosphonate, and aminocarbonylphosphonate were synthesized. most of them were shown to inhibit the replication of type 1 herpes simplex virus in vero cell culture. the stability in phosphate buffer and human blood serum was studied for several of the derivatives. a correlation between the stability and antiviral activity of the synthesized compounds is discussed. the english version of the paper: russian journal ... | 2004 | 15586811 |
| synthesis and evaluation of 2'-deoxy-2'-18f-fluoro-5-fluoro-1-beta-d-arabinofuranosyluracil as a potential pet imaging agent for suicide gene expression. | 2'-deoxy-2'-(18)f-fluoro-5-fluoro-1-beta-d-arabinofuranosyluracil ((18)f-ffau) has been synthesized and evaluated in ht-29 cells as a potential pet agent for herpes simplex virus type 1 thymidine kinase (hsv1-tk) gene expression. | 2004 | 15585483 |
| expression of il-15, il-18 and nos-ii in contralateral eyes of balb/c mice during the development of hsv-induced keratitis. | 2005 | 15585336 | |
| eruptive disseminated superficial actinic porokeratosis in an immunocompetent host: is this associated with herpes simplex virus or bacterial infection? | 2004 | 15583605 | |
| in vitro and in vivo antiherpetic activity of three new synthetic brassinosteroid analogues. | brassinosteroids are a novel group of steroids that appear to be ubiquitous in plants and are essential for normal plant growth and development. it has been previously reported that brassinosteroid analogues exert an antiviral activity against herpes simplex virus type 1 (hsv-1) and arenaviruses. in the present study, we report the chemical synthesis of compounds (22s,23s)-3beta-bromo-5alpha,22,23-trihydroxystigmastan-6-one (2), (22s,23s)-5alpha-fluoro-3beta-22,23-trihydroxystigmastan-6-one (3), ... | 2004 | 15579323 |
| detection of herpes simplex virus, cytomegalovirus and epstein-barr virus dna in atherosclerotic plaques and in unaffected bypass grafts. | herpes virus infections are suspected to be involved in the pathogenesis of atherosclerosis. | 2005 | 15572003 |
| docosanol: a topical antiviral for herpes labialis. | recurrent herpes labialis is a painful and potentially disfiguring infection affecting an estimated 40 million people in the us alone. the majority of recurrences are caused by herpes simplex virus type 1. various oral and topical formulations of nucleoside analogues have demonstrated efficacy for this indication. over-the-counter treatments are palliative in nature and do not reduce time to healing. docosanol is a compound with a unique mechanism of action involving viral fusion inhibition. in ... | 2004 | 15571473 |
| methylprednisolone, valacyclovir, or both for vestibular neuritis. | 2004 | 15570683 | |
| intimate male partner violence impairs immune control over herpes simplex virus type 1 in physically and psychologically abused women. | intimate partner violence (ipv) against women is a worldwide problem and a cause of significant distress and threat to health. studies have focused mainly on mental health, and few have considered the effect on physiological systems. the aim of this research was to determine whether ipv also compromises the immune system, as evidenced by a decrease in immune regulation over herpes simplex virus type 1 (hsv-1), the latent virus that causes cold sores. | 2004 | 15564366 |
| prevention of genital herpes in a guinea pig model using a glycoprotein d-specific single chain antibody as a microbicide. | background: genital herpes (gh) is a recurrent sexually transmitted infection (sti) that causes significant morbidity and is also the major source of herpes simplex virus (hsv) in cases of neonatal herpes. vaccination is a current goal which has had limited success so far in preventing gh and microbicides offer an attractive alternative. treatment of primary disease cannot prevent establishment of latent infections and thus, cannot prevent subsequent recurrent disease. recently, many of the mole ... | 2004 | 15560847 |
| viral activation of macrophages through tlr-dependent and -independent pathways. | induction of cytokine production is important for activation of an efficient host defense response. macrophages constitute an important source of cytokines. in this study we have investigated the virus-cell interactions triggering induction of cytokine expression in macrophages during viral infections. we found that viral entry and viral gene products produced inside the cell are responsible for activation of induction pathways leading to ifn-alphabeta expression, indicating that virus-cell inte ... | 2004 | 15557184 |
| antiviral and antiplasmodial spirodihydrobenzofuran terpenes from the fungus stachybotrys nephrospora. | two known spirodihydrobenzofuran terpenes (1 and 2) were isolated from a mycelium extract of the fungus stachybotrys nephrospora bcc 3900. compound 1 (mer-nf5003f or stachybotrydial) exhibited potent antiviral activity (the ic50 value of 4.32 microg/ml) comparable to the standard drug, acyclovir, while compound 2 was inactive against the hsv-1 virus. both 1 and 2 possessed antiplasmodial activity (ic50 values of 0.85 and 0.15 microg/ml for 1 and 2, respectively), and were not toxic towards the v ... | 2004 | 15549667 |
| azaanalogues of ebselen as antimicrobial and antiviral agents: synthesis and properties. | the different analogues of ebselen-unsubstituted benzisoselenazol-3(2h)-one (2a) 2-pyridylbenzisoselenazol-3(2h)-ones (2b-h) and 7-azabenzisoselenazol-3(2h)-ones (3a-j) were designed as new selenium-containing antiviral and antimicrobial agents and synthesized. some of them were found in the antiviral assay in vitro to be strong inhibitors of cythopatic activity of herpes simplex virus type 1--hsv-1 (compounds 2a,b,f,h, 3a-j) and encephalomyocarditis virus--emcv (compounds 2a,h, 3a-f,k,l). the c ... | 2004 | 15544790 |
| 5-(1-substituted) alkyl pyrimidine nucleosides as antiviral (herpes) agents. | the treatment of viral diseases remains one of the major challenges to modern medicine. during the past two decades there has been increased recognition of the consequences of serious viral illnesses that are not controlled by vaccination. these illnesses include human immunodeficiency virus, human herpes viruses, and viruses that cause hepatitis. there are now eight pathogens recognized in the herpes virus family that cause infections in humans. infections by the herpes viruses are opportunisti ... | 2004 | 15544474 |
| vp22-mediated intercellular transport correlates with enhanced biological activity of mybengrailed but not (hsv-i) thymidine kinase fusion proteins in primary vascular cells following non-viral transfection. | the intercellular transport properties of the herpes simplex virus (hsv) protein vp22 have been harnessed to enhance the effectiveness of viral gene transfer. we investigated the intercellular transport and biological effects of vp22 fused with the dominant negative c-myb chimera, mybengrailed (myben) and hsv-i thymidine kinase (tk), in primary vascular smooth muscle cells (vsmc) following non-viral transfection. | 2005 | 15543525 |
| exposure to urban pollutants and serum levels of igg anti herpes simplex virus type 1. | the aim of the study is to investigate whether traffic policemen exposed to urban pollutants and psycho-social stressors may be at risk of modifications in serum levels of immunoglobulins g antibodies (igg ab) against herpes simplex virus (type1) (hsv-1) compared with controls. traffic policemen were matched by sex, age, working life and drinking habits (less than two glasses of wine or beer per day) with controls, after excluding the subjects with the principal confounding factors (smoking habi ... | 2004 | 15540623 |
| hsv-1 vs. hsv-2 demographics. | 2004 | 15536801 | |
| serologic survey in a colony of captive common marmosets (callithrix jacchus) after infection with herpes simplex type 1-like virus. | an outbreak of herpesvirus caused the death of four of five common marmosets (callithrix jacchus) in a private colony. gross lesions included acute ulcerative gingivitis, glossitis, and enlarged mandibular lymph nodes. histologically, all fatal cases showed meningoencephalitis and eosinophilia with intranuclear inclusion bodies in neurons and glial cells. a herpes simplex-like virus was cultured from the brain and was identified as herpes simplex type 1 virus or a closely related virus by immuno ... | 2004 | 15526895 |
| study on the mechanism of bioelectric recognition assay: evidence for immobilized cell membrane interactions with viral fragments. | the bioelectric recognition assay (bera) is a whole-cell based biosensing system that detects the electric response of cultured cells, suspended in a gel matrix, to various ligands, which bind to the cell and/or affect its physiology. previous studies have demonstrated the potential application of this method for rapid, inexpensive detection of viruses in a crude sample. however, the understanding, so far, of the fundamental processes that take place during cell-virus interactions within the pro ... | 2004 | 15522609 |
| acog practice bulletin: clinical management guidelines for obstetrician-gynecologists, number 57, november 2004. gynecologic herpes simplex virus infections. | both herpes simplex virus (hsv) type i and hsv type 2 can cause genital herpes. because the infection is chronic, genital herpes has become the most common sexually transmitted disease among women. the prevalence of the hsv-2 antibody among women in the united states is 26%, although genital herpes has been diagnosed in only a small proportion (10-25%) of individuals with hsv-2 antibodies. herpes simplex virus type i is becoming a more frequent cause of genital herpes, especially among young wom ... | 2004 | 15516420 |
| virulence genes in herpes simplex virus type 1 corneal infection. | 2004 | 15512957 | |
| synthesis and antiviral activity of 3-formyl- and 3-cyano-2,5,6-trichloroindole nucleoside derivatives. | a series of trichlorinated indole nucleosides has been synthesized and tested for activity against human cytomegalovirus (hcmv) and herpes simplex virus type-1 (hsv-1) and for cytotoxicity. the previously reported 3-formyl-2,5,6-trichloro-1-(beta-d-ribofuranosyl)indole (ftcri) and its 3-cyano homologue (ctcri) were chemically modified at the 3-position. the formation of hydrazones and oximes of ftcri was accomplished by a dehydrative addition of the appropriate hydrazine or hydroxylamine derivat ... | 2004 | 15509175 |
| synthesis and antiviral evaluation of polyhalogenated imidazole nucleosides: dimensional analogues of 2,5,6-trichloro-1-(beta-d-ribofuranosyl)benzimidazole. | a series of polyhalogenated imidazole nucleosides were designed and synthesized as ring-contracted analogues of 2,5,6-trichloro-1-(beta-d-ribofuranosyl)benzimidazole (tcrb) and its 2-bromo analogue (bdcrb) in an effort to explore the spatial limitation of the active pocket(s) in the target protein(s). 2,4,5-trichloro-, 2-bromo-4,5-dichloro-, and 2,4,5-tribromoimidazole nucleosides were prepared by a condensation of the preformed heterocycles with the appropriate sugar precursors. the ribofuranos ... | 2004 | 15509173 |
| ftir microscopy detection of cells infected with viruses. | fourier-transform infrared (ftir) microscopy is considered a comprehensive and sensitive method for detection of molecular changes in cells. the advantage of ftir microspectroscopy over conventional ftir spectroscopy is that it facilitates inspection of restricted regions of a cell culture or a tissue. we have shown that it is possible to apply ftir microscopy as a sensitive and effective assay for the detection of cells infected with various members of the herpes family of viruses and retroviru ... | 2005 | 15507707 |
| persistent expression of chemokine and chemokine receptor rnas at primary and latent sites of herpes simplex virus 1 infection. | inflammatory cytokines and infiltrating t cells are readily detected in herpes simplex virus (hsv) infected mouse cornea and trigeminal ganglia (tg) during the acute phase of infection, and certain cytokines continue to be expressed at lower levels in infected tg during the subsequent latent phase. recent results have shown that hsv infection activates toll-like receptor signaling. thus, we hypothesized that chemokines may be broadly expressed at both primary sites and latent sites of hsv infect ... | 2004 | 15507126 |
| a wide range of medium-sized, highly cationic, alpha-helical peptides show antiviral activity against herpes simplex virus. | ten highly cationic, alpha-helical peptides were synthesized and tested for antiviral activity against herpes simplex virus 1 and 2 (hsv-1 and hsv-2). several of the peptides were found to exhibit antiviral activity. the peptides affinity for heparan sulfate (hs) increased with the number of cationic residues. net charge could be decisive for the anti-hsv-1 activity, while secondary structure of the peptides seems more important for the anti-hsv-2 activity. the peptides were able to inhibit the ... | 2004 | 15498607 |
| htrp--an immediate-early gene product induced by hsv1 infection in human embryo fibroblasts, is involved in cellular co-repressors. | the interaction between virus and receptor is a process that mimics physiological ligand binding receptors and induces signal transduction. in the investigation of the interaction between hsv1 (herpes simplex virus 1) and human fibroblasts via virus binding to its receptor complex on cellular membranes, the htrp (human transcription regulator protein), a protein encoded by an immediate-early gene of cellular response against the specific stimulation of hsv1 binding, was cloned from a cdna librar ... | 2004 | 15496587 |
| 6-azapyrimidine-2'-deoxy-4'-thionucleosides: antiviral agents against tk+ and tk- hsv and vzv strains. | the synthesis of a series of novel 1-(2-deoxy-4-thio-beta-d-erythro-pentofuranosyl)-(6-azapyrimidine) nucleosides is described. x-ray crystallographic data of the thymidine derivative allowed conformational analysis, which indicated a twist (3t2) sugar conformation. hydrogen-bonded assemblies for the crystal structure were determined using platon software to allow further interpretation of the crystal packing and base interactions. the 6-azapyrimidine nucleosides described were evaluated against ... | 2004 | 15481985 |
| antiviral activities of medicinal herbs traditionally used in southern mainland china. | the aqueous extracts from 21 medicinal herbs traditionally used in southern mainland china were screened for antiviral activities against human herpes simplex virus type 1 (hsv-1) and human respiratory syncytial virus (rsv) using a cytopathic effect (cpe) reduction assay. three extracts from agrimonia pilosa, pithecellobium clypearia and punica granatum, respectively, showed anti-hsv-1 activity, which was possibly contributed by the polyphenolic compounds in the herbal extracts. six of the extra ... | 2004 | 15478204 |
| inhibition of the stress-activated kinase, p38, does not affect the virus transcriptional program of herpes simplex virus type 1. | to investigate the impact of stress kinase p38 activation on hsv-1 transcription, we performed a global transcript profile analysis of viral mrna using an oligonucleotide-based dna microarray. rna was isolated from vero cells infected with the kos strain of hsv-1 in the presence or absence of sb203580, a pyridinyl imidazole inhibitor of p38. under conditions that eliminated atf2 activation but had no effect on c-jun, and reduced virus yield by 85-90%, no effect on accumulation of viral ie, de, o ... | 2004 | 15476882 |
| cognate cd4(+) t cell licensing of dendritic cells in cd8(+) t cell immunity. | several studies have indicated that cd8(+) t cells require cd4(+) t cell help for memory formation. evidence suggests that such help can be antigen independent, challenging whether the 'licensing' of dendritic cells (dcs) by cd4(+) t cells is ever required for cytotoxic t lymphocyte (ctl) responses. we show here that help is essential for the generation of ctl immunity to herpes simplex virus 1 and that cd4(+) t cells mediate help in a cognate, antigen-specific way. we provide direct in vivo evi ... | 2004 | 15475958 |
| a new lignan glycoside and phenylethanoid glycosides from strobilanthes cusia bremek. | the root of strobilanthes cusia bremek. (acanthaceae), popularly known as da-ching-yeh, has been commonly used in traditional chinese medicine. it is used to treat influenza, epidemic cerebrospinal meningitis, encephalitis b, viral pneumonia, mumps, and severe acute respiratory syndrome (sars). in this study, we found a new lignan glycoside (6) and two new phenylethanoid glycosides (7, 8) together with five known compounds as chemical constituents of strobilanthes cusia root. some samples were e ... | 2004 | 15467245 |
| microarray analysis in the hsv-1 latently infected mouse trigeminal ganglion. | to review our previous studies regarding alterations in gene expression in hsv-1 latently infected mouse trigeminal ganglia (tgs) following treatment with immunosuppressants and hyperthermia. | 2004 | 15448479 |
| (e)-5-(2-bromovinyl)-2'-deoxyuridine (bvdu). | (e)-5-(2-bromovinyl)-2'-deoxyuridine (bvdu, brivudin, zostex, zerpex, zonavir), now more than 20 years after its discovery, still stands out as a highly potent and selective inhibitor of herpes simplex virus type 1 (hsv-1) and varicella-zoster virus (vzv) infections. it has been used in the topical treatment of herpetic keratitis and recurrent herpes labialis and the systemic (oral) treatment of herpes zoster (zona, shingles). the high selectivity of bvdu towards hsv-1 and vzv depends primarily ... | 2005 | 15389733 |
| hesseltin a, a novel antiviral metabolite from penicillium hesseltinei. | [structure: see text] hesseltin a 1, a novel compound of mixed polyketide-terpenoid origins was isolated from the filamentous fungus penicillium hesseltinei. the structure and stereochemistry were determined from extensive one- and two-dimensional nmr and mass spectral data. | 2004 | 15387518 |
| short interfering rna-mediated inhibition of herpes simplex virus type 1 gene expression and function during infection of human keratinocytes. | rna interference (rnai) is an antiviral mechanism that is activated when double-stranded rna is cleaved into fragments, called short interfering rna (sirna), that prime an inducible gene silencing enzyme complex. we applied rnai against a herpes simplex virus type 1 (hsv-1) gene, glycoprotein e, which mediates cell-to-cell spread and immune evasion. in an in vitro model of infection, human keratinocytes were transfected with sirna specific for glycoprotein e and then infected with wild-type hsv- ... | 2004 | 15367593 |
| increased presence of matrix metalloproteinases 2 and 9 in short- and long-term experimental herpes simplex virus encephalitis. | herpes simplex virus encephalitis (hsve) causes elevated morbidity and mortality despite antiviral treatment. virus-independent mechanisms may perpetuate brain damage. matrix metalloproteinases (mmps) target extracellular matrix components. this study describes the protein and mrna expression of mmp2 and mmp9 in experimental hsve in the short and long term. ten sjl-nbom mice were infected with neurovirulent hsv-1 and compared with nine controls. the presence of mmp2 and mmp9 in brain tissue was ... | 2004 | 15364410 |
| inhibition of herpesvirus-induced hiv-1 replication by cyclopentenone prostaglandins: role of ikappab kinase (ikk). | herpes simplex virus (hsv) infections have been associated with reactivation of hiv-1 replication and increases of hiv-1-load in plasma of co-infected individuals. the present authors have previously reported that in epithelial cells hsv-1 induces the ikappab-kinase (ikk) causing persistent activation of nf-kappab, a critical regulator of hiv-1 replication. the present study was performed to investigate whether hsv-1-infection could induce ikk-mediated nf-kappab activation and enhance hiv-1 expr ... | 2004 | 15362659 |
| delayed facial paralysis after vestibular schwannoma surgery: role of herpes viruses reactivation--our experience in eight cases. | the objectives of this study were to study the role of herpes virus reactivation in the onset of delayed facial paralysis (dfp) occurring more than 72 hours after vestibular schwannoma (vs) surgery and to advocate specific medical management. | 2004 | 15354015 |
| synthesis and evaluation of thymine-derived carboxamides against mitochondrial thymidine kinase (tk-2) and related enzymes. | based on the structure of our previously identified mitochondrial thymidine kinase (tk-2) inhibitors, three series of thymine-derived carboxamides have been synthesized and tested against tk-2 and related enzymes. the methodology employed has been a solution-phase parallel synthesis based on the coupling of three thymine-derived acids [4-(thymin-1-yl)butyric acid (i), [4-(thymin-1-yl)-butyrylamino]acetic acid (ii) and 6-(thymin-1-yl)hexanoic acid (iii)] with different commercially available prim ... | 2004 | 15351391 |
| enhancing sensitivity of human herpes virus diagnosis with dna microarrays using dendrimers. | dna microarray technology has become a promising new tool for the detection and identification of viral pathogens in human plasma and cell cultures. for exploration of this technology, we have developed dna microarrays that encode capture oligonucleotide probes for different human herpes viruses: herpes simplex virus (hsv) hsv-1, hsv-2, varicella zoster virus (vzv), epstein-barr virus (ebv), cytomegalovirus (cmv), and hhv-6. the on-chip hybridization is accomplished with the pcr amplicons of the ... | 2004 | 15351231 |
| [do antisense oligonucleotides improve viral immunopathology?]. | tumor necrosis factor (tnf)-alpha as a proinflammatory cytokine is of great importance during the development of herpes simplex virus-1 keratitis (hsk). in this study the local administration of antisense oligonucleotides (ason) targeting tnf-alpha was examined for its usefulness in ameliorating this disease. | 2005 | 15340755 |
| [establishment of primary cultured neurons model of mouse embryo with hsv-1 infection]. | to establish a neurons model for herpes simplex virus type 1 (hsv-1) infection using cultures of mouse embryo. | 2003 | 15340566 |