Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| confocal epifluorescence sensor with an arc-shaped aperture for slide-based pcr quantification. | the increasing needs of point-of-care diagnostics, quarantine of epidemic pathogens, and prevention of terrorism's bio-attacks have promised the future of portable real-time quantitative polymerase chain reaction (qpcr) sensors. this work aims at developing a highly sensitive and low-cost light emitting diode (led)-based epifluorescence sensor module for qpcr sensor development and relevant bioassay applications. inspired by the light stop design and dark-field detection of microscopes, this pap ... | 2017 | 28863326 |
| the probiotic combination of bifidobacterium longum subsp. infantis cect 7210 and bifidobacterium animalis subsp. lactis bpl6 reduces pathogen loads and improves gut health of weaned piglets orally challenged with salmonella typhimurium. | probiotics have been demonstrated to be useful to enhance gut health and prevent gastrointestinal infections in humans. additionally, some multi-strain probiotic combinations have been suggested to have greater efficacy than single strains. the objective of this study is to demonstrate the potential of a combination of the probiotic strains: bifidobacterium longum subsp. infantis cect 7210 (brand name b. infantis im1(®)) and b. animalis subsp. lactis bpl6 to enhance gut health and to ameliorate ... | 2017 | 28861074 |
| epithelial cell damage activates bactericidal/permeability increasing-protein (bpi) expression in intestinal epithelium. | as the first line of defense against invading pathogen, intestinal epithelium produces various antimicrobial proteins (amp) that help in clearance of pathogen. bactericidal/permeability-increasing protein (bpi) is a 55 kda amp that is expressed in intestinal epithelium. dysregulation of bpi in intestinal epithelium is associated with various inflammatory diseases like crohn's disease, ulcerative colitis, and infectious enteritis's. in this paper, we report a direct correlation between intestinal ... | 2017 | 28861073 |
| development of gold nanoparticle-aptamer-based lspr sensing chips for the rapid detection of salmonella typhimurium in pork meat. | a non-labeled, portable plasmonic biosensor-based device was developed to enable the ultra-sensitive and selective detection of salmonella typhimurium in pork meat samples. specifically, a plasmonic sensor, using the self-assembly of gold nanoparticles (aunps) to achieve a regulated diameter of 20 nm for the aunp monolayers, was used to conduct high-density deposition on a transparent substrate, which produced longitudinal wavelength extinction shifts via a localized surface plasmon resonance (l ... | 2017 | 28860462 |
| complete genome sequence of salmonella enterica lytic bacteriophage lpst10, isolated in china. | bacteriophage lpst10 was isolated from wuhan, china. lytic activity was demonstrated against multiple salmonella enterica serovars, including salmonella enterica serovar typhimurium strains. this bacteriophage has a 47,657-bp double-stranded dna genome encoding 87 putative coding sequences. | 2017 | 28860234 |
| protein energy malnutrition alters mucosal iga responses and reduces mucosal vaccine efficacy in mice. | oral vaccine responsiveness is often lower in children in less developed countries. childhood malnutrition may be associated with poor immune response to oral vaccines. the present study was designed to investigate whether protein energy malnutrition (pem) impairs b cell immunity and ultimately reduces oral vaccine efficacy in a mouse model. purified isocaloric diets containing low protein (1/10 the protein of the control diet) were used to determine the effect of pem. pem increased both nonspec ... | 2017 | 28860040 |
| salmonella enterica serovar typhi siderophore production is elevated and fur inactivation causes cell filamentation and attenuation in macrophages. | salmonella enterica serovars typhi and typhimurium are two closely related bacteria causing different types of infection in humans. iron acquisition is considered essential for virulence. siderophores are important iron chelators and production of enterobactin and salmochelins by these serovars was quantified. overall, salmonella typhi produced higher levels of siderophores than salmonella typhimurium. the role of the global regulator fur, involved in iron homeostasis, present and conserved in b ... | 2017 | 28859315 |
| in vitro antimicrobial activity screening of xylaria hypoxylon. | fungi have a potential of using both as nutritive and medicinal food stuff. because of containing several therapeutic agents, they are reported to be used for hundreds of years to treat several diseases caused by bacteria, fungi, viruses and parasites. the aim of this study is to determine the in vitro antimicrobial activity of xylaria hypoxylon, which were collected from yomra, trabzon, turkey. | 2016 | 28852719 |
| comparative genomics of australian and international isolates of salmonella typhimurium: correlation of core genome evolution with crispr and prophage profiles. | salmonella enterica subsp enterica serovar typhimurium (s. typhimurium) is a serovar with broad host range. to determine the genomic diversity of s. typhimurium, we sequenced 39 isolates (37 australian and 2 uk isolates) representing 14 repeats groups (rgs) determined primarily by clustered regularly interspaced short palindromic repeats (crispr). analysis of single nucleotide polymorphisms (snps) among the 39 isolates yielded an average of 1,232 snps per isolate, ranging from 128 snps to 11,339 ... | 2017 | 28851865 |
| a portable impedance immunosensing system for rapid detection of salmonella typhimurium. | salmonellatyphimurium is one of the most dangerous foodborne pathogens and poses a significant threat to human health. the objective of this study was to develop a portable impedance immunosensing system for rapid and sensitive detection of s. typhimurium in poultry. the developed portable impedance immunosensing system consisted of a gold interdigitated array microelectrode (idam), a signal acquisitive interface and a laptop computer with labview software. the idam was first functionalized with ... | 2017 | 28846643 |
| whole-genome sequences of salmonella enterica subsp. enterica serovar typhimurium strains tt6675 and tt9097 employed in the isolation and characterization of a giant phage mutant collection. | we report here the genome sequences of salmonella enterica subsp. enterica serovar typhimurium strains tt6675 and tt9097, which we utilize for genetic analyses of giant bacterial viruses. our analyses identified several genetic variations between the two strains, most significantly confirming strain tt6675 as a serine suppressor and tt9097 as a nonsuppressor. | 2017 | 28839032 |
| use of multiple-locus variable-number of tandem repeats analysis (mlva) to investigate genetic diversity of salmonella enterica subsp. enterica serovar typhimurium isolates from human, food, and veterinary sources. | salmonella enterica subspecies enterica serovar typhimurium is the most common zoonotic pathogen in bulgaria. to allow efficient outbreak investigations and surveillance in the food chain, accurate and discriminatory methods for typing are needed. this study evaluated the use of multiple-locus variable-number of tandem repeats analysis (mlva) and compared results with antimicrobial resistance (amr) determinations for 100 s. typhimurium strains isolated in bulgaria during 2008-2012 (50 veterinary ... | 2017 | 28836358 |
| resistance mechanisms and fitness of salmonella typhimurium and salmonella enteritidis mutants evolved under selection with ciprofloxacin in vitro. | the aim of this study was to investigate the difference in resistance mechanisms and fitness of salmonella typhimurium (st) and salmonella enteritidis (se) mutants selected during the evolution of resistance under exposure to increasing ciprofloxacin concentrations in vitro. mutations in quinolone target genes were screened by pcr. phenotypic characterization included susceptibility testing by the broth dilution method, investigation of efflux activity and growth rate, and determination of the i ... | 2017 | 28831084 |
| analysis of spleen-induced fimbria production in recombinant attenuated salmonella enterica serovar typhimurium vaccine strains. | salmonella enterica serovar typhimurium genome encodes 13 fimbrial operons. most of the fimbriae encoded by these operons are not produced under laboratory conditions but are likely to be synthesized in vivo we used an in vivo expression technology (ivet) strategy to identify four fimbrial operons, agf, saf, sti, and stc that are expressed in the spleen. when any three of these operons were deleted, the strain retained wild-type virulence. however, when all four operons were deleted, the resulti ... | 2017 | 28830946 |
| interleukin-10 production by t and b cells is a key factor to promote systemic salmonella enterica serovar typhimurium infection in mice. | salmonella enterica serovar typhimurium (s. typhimurium) is a gram-negative bacterium that produces disease in numerous hosts. in mice, oral inoculation is followed by intestinal colonization and subsequent systemic dissemination, which leads to severe pathogenesis without the activation of an efficient anti-salmonella immune response. this feature suggests that the infection caused by s. typhimurium may promote the production of anti-inflammatory molecules by the host that prevent efficient t c ... | 2017 | 28824622 |
| pathobactyper: a web server for pathogenic bacteria identification and molecular genotyping. | with the decline in the cost of whole-genome sequencing because of the introduction of next-generation sequencing (ngs) techniques, many public health and clinical laboratories have started to use bacterial whole genomes for epidemiological surveillance and clinical investigation. for epidemiological and clinical purposes in this "ngs era," whole-genome-scale single nucleotide polymorphism (wgsnp) analysis for genotyping is considered suitable. in this paper, we present an online service, pathob ... | 2017 | 28824598 |
| comparison of the microbiological quality and safety between conventional and organic vegetables sold in malaysia. | given the remarkable increase of public interest in organic food products, it is indeed critical to evaluate the microbiological risk associated with consumption of fresh organic produce. organic farming practices including the use of animal manures may increase the risk of microbiological contamination as manure can act as a vehicle for transmission of foodborne pathogens. this study aimed to determine and compare the microbiological status between organic and conventional fresh produce at the ... | 2017 | 28824567 |
| in vitro toxicological assessment of arrabidaea brachypoda (dc.) bureau: mutagenicity and estrogenicity studies. | arrabidaea brachypoda (dc.) bureau is a shrub native cerrado, known as "cipó-una", "tintureiro" or "cervejinha do campo" and popularly used in southeastern and northeastern brazil to treatment of kidney stones and painful joints (arthritis). nevertheless, scientific information regarding this species is scarce, and there are no reports related to its possible estrogenic and mutagenic effects. thus, the principal objective of this study was to assess the mutagenic and estrogenic activities of the ... | 2017 | 28823851 |
| lrrk2 promotes the activation of nlrc4 inflammasome during salmonella typhimurium infection. | although genetic polymorphisms in the lrrk2 gene are associated with a variety of diseases, the physiological function of lrrk2 remains poorly understood. in this study, we report a crucial role for lrrk2 in the activation of the nlrc4 inflammasome during host defense against salmonella enteric serovar typhimurium infection. lrrk2 deficiency reduced caspase-1 activation and il-1β secretion in response to nlrc4 inflammasome activators in macrophages. lrrk2(-/-) mice exhibited impaired clearance o ... | 2017 | 28821568 |
| anti-biofilm effects of anthranilate on a broad range of bacteria. | anthranilate, one of tryptophan degradation products has been reported to interfere with biofilm formation by pseudomonas aeruginosa. here, we investigated the effects of anthranilate on biofilm formation by various bacteria and the mechanisms responsible. anthranilate commonly inhibited biofilm formation by p. aeruginosa, vibrio vulnificus, bacillus subtilis, salmonella enterica serovar typhimurium, and staphylococcus aureus, and disrupted biofilms preformed by these bacteria. because anthranil ... | 2017 | 28819217 |
| use of whey as a culture medium for bacillus clausii for the production of protein hydrolysates with antimicrobial and antioxidant activity. | the use of whey as a natural culture medium to produce hydrolysates with antimicrobial from bacillus clausii is the objective of this research. the fermentation process was carried out at 25 ℃ for five days, where proteolytic activity, soluble peptides quantification, and antimicrobial activity using the disc diffusion method were determined every day. the fermented whey reached values of proteolytic activity between 90 and 260 u ml(-1) and a production of soluble peptides between 100 and 2070 ... | 2017 | 28816527 |
| synergistic antibacterial and antibiofilm efficacy of nisin in combination with p-coumaric acid against foodborne bacteria bacillus cereus and salmonella typhimurium. | the aim of the study was to evaluate possible antibacterial and antibiofilm efficacy of a bacteriocin, nisin with two essential oil components linalool and p-coumaric acid in combination against foodborne bacteria bacillus cereus and salmonella typhimurium. their inhibition effects on planktonic cells and pre-formed biofilms were evaluated using microbroth dilution and checkerboard titration methods. nisin/ p-coumaric acid combination showed synergistic effects against planktonic cells of both t ... | 2017 | 28815637 |
| the antibacterial and antifungal activity of six essential oils and their cyto/genotoxicity to human hel 12469 cells. | six essential oils (from oregano, thyme, clove, lavender, clary sage, and arborvitae) exhibited different antibacterial and antifungal properties. antimicrobial activity was shown against pathogenic (escherichia coli, salmonella typhimurium, yersinia enterocolitica, staphylococcus aureus, listeria monocytogenes, and enterococcus faecalis) and environmental bacteria (bacillus cereus, arthrobacter protophormiae, pseudomonas fragi) and fungi (chaetomium globosum, penicillium chrysogenum, cladospori ... | 2017 | 28811611 |
| short communication: detection of stx2 and elt genes in bovine milk by using a multiplex pcr system. | the aim of this study was to detect 2 important toxin genes from diarrheagenic escherichia coli (dec) in bovine milk using a new multiplex pcr. to standardize the multiplex pcr, the stx2 and elt genes were investigated for the detection of shiga toxin-producing escherichia coli (stec) and enterotoxigenic e. coli (etec), respectively. the dna template was prepared with a thermal procedure (boiling) and a commercial kit. samples consisted of uht and pasteurized milk, both skimmed, and stec and ete ... | 2017 | 28803013 |
| estimation of the probability of bacterial population survival: development of a probability model to describe the variability in time to inactivation of salmonella enterica. | despite the development of numerous predictive microbial inactivation models, a model focusing on the variability in time to inactivation for a bacterial population has not been developed. additionally, an appropriate estimation of the risk of there being any remaining bacterial survivors in foods after the application of an inactivation treatment has not yet been established. here, gamma distribution, as a representative probability distribution, was used to estimate the variability in time to ... | 2017 | 28800819 |
| transcriptome and proteome analysis of salmonella enterica serovar typhimurium systemic infection of wild type and immune-deficient mice. | salmonella enterica are a threat to public health. current vaccines are not fully effective. the ability to grow in infected tissues within phagocytes is required for s. enterica virulence in systemic disease. as the infection progresses the bacteria are exposed to a complex host immune response. consequently, in order to continue growing in the tissues, s. enterica requires the coordinated regulation of fitness genes. bacterial gene regulation has so far been investigated largely using exposure ... | 2017 | 28796780 |
| 6s rna is involved in acid resistance and invasion of epithelial cells in salmonella enterica serovar typhimurium. | acid is an important environmental condition encountered frequently by salmonella enterica serovar typhimurium during its pathogenesis, but the role of small-noncoding rnas (srnas) in response to acid stress is poorly understood. | 2017 | 28796533 |
| the microbiome activates cd4 t-cell-mediated immunity to compensate for increased intestinal permeability. | despite a prominent association, chronic intestinal barrier loss is insufficient to induce disease in human subjects or experimental animals. we hypothesized that compensatory mucosal immune activation might protect individuals with increased intestinal permeability from disease. we used a model in which intestinal barrier loss is triggered by intestinal epithelial-specific expression of constitutively active myosin light chain kinase (ca-mlck). here we asked whether constitutive tight junction ... | 2017 | 28795125 |
| vitamin b12 as a carrier of peptide nucleic acid (pna) into bacterial cells. | short modified oligonucleotides targeted at bacterial dna or rna could serve as antibacterial agents provided that they are efficiently taken up by bacterial cells. however, the uptake of such oligonucleotides is hindered by the bacterial cell wall. to overcome this problem, oligomers have been attached to cell-penetrating peptides, but the efficiency of delivery remains poor. thus, we have investigated the ability of vitamin b12 to transport peptide nucleic acid (pna) oligomers into cells of es ... | 2017 | 28794451 |
| rpon2- and flia-regulated flitx is indispensible for flagellar motility and virulence in xanthomonas oryzae pv. oryzae. | bacterial blight of rice caused by xanthomonas oryzae pv. oryzae (xoo) is one of the most important crop diseases in the world. more insights into the mechanistic regulation of bacterial pathogenesis will help us identify novel molecular targets for developing effective disease control strategies. a large flagellar gene cluster is regulated under a three-tiered hierarchy by σ(54) factor rpon2 and its activator fleq, and σ(28) factor flia. a hypothetical protein gene flitx is located upstream of ... | 2017 | 28793882 |
| assessment of cellular mutagenicity of americano coffees from popular coffee chains. | coffee is a popular beverage worldwide, but coffee beans can be contaminated with carcinogens. the ames salmonella mutagenicity test is often used for analysis of carcinogens for mutagenicity. however, previous studies have provided controversial data about the direct mutagenicity of coffee beans based on ames test results. this study was conducted to determine the mutagenicity of popular americano coffee based on results from the ames test. coffee samples without additives that were served by f ... | 2017 | 28792232 |
| what the sif is happening-the role of intracellular salmonella-induced filaments. | a common strategy among intracellular bacterial pathogens is to enter into a vacuolar environment upon host cell invasion. one such pathogen, salmonella enterica, resides within the salmonella-containing vacuole (scv) inside epithelial cells and macrophages. salmonella hijacks the host endosomal system to establish this unique intracellular replicative niche, forming a highly complex and dynamic network of salmonella-induced filaments (sifs). sifs radiate outwards from the scv upon onset of bact ... | 2017 | 28791257 |
| milk fermented by lactobacillus species from brazilian artisanal cheese protect germ-free-mice against salmonella typhimurium infection. | ingestion of milks fermented by lactobacillus strains showing probiotic properties is an important tool to maintain gastrointestinal health. in this study, lactobacillus rhamnosus d1 and lactobacillus plantarum b7, isolated from brazilian artisanal cheese, were used as starters for the functional fermented milks to assess their probiotic properties in a gnotobiotic animal model. male germ-free swiss mice received a single oral dose of milk fermented by each sample, and were challenged with salmo ... | 2017 | 28789560 |
| insights from protein-protein interaction studies on bacterial pathogenesis. | the threat bacterial pathogens pose to human health is increasing with the number and distribution of antibiotic-resistant bacteria, while the rate of discovery of new antimicrobials dwindles. proteomics is playing key roles in understanding the molecular mechanisms of bacterial pathogenesis, and in identifying disease outcome determinants. the physical associations identified by proteomics can provide the means to develop pathogen-specific treatment methods that reduce the spread of antibiotic ... | 2017 | 28786313 |
| heterologous prime-boost vaccination enhances tspmy's protective immunity against trichinella spiralis infection in a murine model. | tspmy is a paramyosin expressed by parasitic trichinella spiralis and confers a protective immunity when its recombinant protein or dna was used as an immunogen. to improve its immunogenicity and vaccine efficacy, we conducted a heterologous prime-boost strategy by orally delivering one dose of tspmy dna carried by attenuated salmonella typhimurium (sl7207), followed by two doses of recombinant tspmy intramuscularly. this strategy effectively induced intestinal mucosal siga response and an enhan ... | 2017 | 28785255 |
| modeling the heat inactivation of foodborne pathogens in milk powder: high relevance of the substrate water activity. | due to the ability of foodborne pathogens to survive in low moisture foods, the decontamination of these products is an important issue in food hygiene. up to now, such decontamination has mostly been achieved through empirical methods. the intention of this work is to establish a more rational use of heat treatment cycles. the effects of thermal treatment cycles on the inactivation of dried salmonella typhimurium, salmonella senftenberg, cronobacter sakazakii and escherichia coli were assessed. ... | 2017 | 28784519 |
| virulence of invasive salmonella typhimurium st313 in animal models of infection. | salmonella typhimurium sequence type (st) 313 produces septicemia in infants in sub-saharan africa. although there are known genetic and phenotypic differences between st313 strains and gastroenteritis-associated st19 strains, conflicting data about the in vivo virulence of st313 strains have been reported. to resolve these differences, we tested clinical salmonella typhimurium st313 and st19 strains in murine and rhesus macaque infection models. the 50% lethal dose (ld50) was determined for thr ... | 2017 | 28783750 |
| circulating and tissue-resident cd4+ t cells with reactivity to intestinal microbiota are abundant in healthy individuals and function is altered during inflammation. | interactions between commensal microbes and the immune system are tightly regulated and maintain intestinal homeostasis, but little is known about these interactions in humans. we investigated responses of human cd4(+) t cells to the intestinal microbiota. we measured the abundance of t cells in circulation and intestinal tissues that respond to intestinal microbes and determined their clonal diversity. we also assessed their functional phenotypes and effects on intestinal resident cell populati ... | 2017 | 28782508 |
| antibiotic resistance patterns and serotypes of salmonella spp. isolated at jeollanam-do in korea. | few long-term studies have been conducted on the serotype and antibiotic resistance patterns of salmonella speices (spp.) the aim of this study was to determine the serotypes and antibiotic resistance patterns of salmonella spp. isolated at jeollanam-do in korea from 2004 to 2014. | 2017 | 28781944 |
| a third family of allelic hsd genes in salmonella enterica: sequence comparisons with related proteins identify conserved regions implicated in restriction of dna. | salmonella enterica serovar blegdam has a restriction and modification system encoded by genes linked to serb. we have cloned these genes, putative alleles of the hsd locus of escherichia coli k-12, and confirmed by the sequence similarities of flanking dna that the hsd genes of s. enterica serovar blegdam have the same chromosomal location as those of e. coli k-12 and salmonella enterica serovar typhimurium lt2. there is, however, no obvious similarity in their nucleotide sequences, and while t ... | 1996 | 28777503 |
| crystal structure of the putative cytoplasmic protein stm0279 (hcp2) from salmonella typhimurium. | stm0279 is a putative cytoplasmic protein from salmonella typhimurium and was recently renamed haemolysin co-regulated protein 2 (hcp2), with the neighbouring stm0276 being hcp1. both of them are encoded by the type vi secretion system (t6ss) of the salmonella pathogenicity island 6 (spi-6) locus and have high sequence identity. the hcp proteins may function as a vital component of the t6ss nanotube and as a transporter and chaperone of diverse effectors from the bacterial t6ss. in this study, t ... | 2017 | 28777089 |
| expression of the gene encoding the major bacterial nucleoid protein h-ns is subject to transcriptional auto-repression. | expression of a promoterless cat gene fused to a dna fragment of approximately 400 bp, beginning at -313 of escherichia coli hns, was significantly repressed in e. coli and salmonella typhimurium strains with wild-type hns but not in mutants carrying hns alleles. cat expression from fusions containing a shorter (110 bp) segment of hns was essentially unaffected in the same genetic backgrounds. the stage of growth was found to influence the extent of repression which was maximum (approximately 75 ... | 1993 | 28776853 |
| treatment of neuroblastoma with an engineered "obligate" anaerobic salmonella typhimurium strain yb1. | purpose neuroblastoma is an embryonic solid tumor derived from the progenitors of the sympathetic nervous system. more than half of the patients developed metastatic disease at the time of initial diagnosis and had poor outcome with current therapeutic approaches. in recent years, some obligate and facultative anaerobic bacteria were reported to target the hypoxic and necrotic region of solid tumor models and caused tumor regression. we recently successfully constructed an "obligate" anaerobic s ... | 2017 | 28775780 |
| epidemiological study of enteropathogenic carrier state among food handlers of armed forces eating establishments. | a cross-sectional epidemiological study was undertaken on a randomly selected sample of 308 food handlers working in different eating establishments of indian armed forces in pune. these included 101 service personnel and 207 civilians. stool samples were analysed for enteropathogens and epidemiological assessment of gastrointestinal disease occurrence in the military units of these food handlers was undertaken. the study revealed that 4 of the subjects were carriers of salmonella typhimurium, t ... | 1999 | 28775561 |
| draft genome sequences of salmonella enterica serovar typhimurium lt2 with deleted chitinases that are emerging virulence factors. | chitinases are glycosyl hydrolases that catalyze the hydrolysis of the β-1,4 linkages in complex carbohydrates and those that contain glcnac. these enzymes are considered emerging virulence factors during infection because the host glycan changes. this is the release of four single chitinase deletion mutants in salmonella enterica serovar typhimurium lt2. | 2017 | 28774970 |
| biosynthesized silver nanoparticles: are they effective antimicrobials? | silver nanoparticles (agnps) are increasingly being used in medical applications. therefore, cost effective and green methods for generating agnps are required. | 2017 | 28767978 |
| participation of 14-3-3ε and 14-3-3ζ proteins in the phagocytosis, component of cellular immune response, in aedes mosquito cell lines. | better knowledge of the innate immune system of insects will improve our understanding of mosquitoes as potential vectors of diverse pathogens. the ubiquitously expressed 14-3-3 protein family is evolutionarily conserved from yeast to mammals, and at least two isoforms of 14-3-3, the ε and ζ, have been identified in insects. these proteins have been shown to participate in both humoral and cellular immune responses in drosophila. as mosquitoes of the genus aedes are the primary vectors for arbov ... | 2017 | 28764795 |
| phenolic acids and flavonoids of peanut by-products: antioxidant capacity and antimicrobial effects. | peanut skin (ps) and meal from dry-blanched peanuts (mdbp) were evaluated as sources of phenolic compounds. ps rendered the highest total phenolic content, antioxidant capacity towards abts radical cation, dpph and hydroxyl radicals as well as reducing power. phenolic acids were present in ps and mdbp whereas proanthocyanidins and monomeric flavonoids were found only in ps as identified by hplc-dad-esi-ms(n). procyanidin-rich extracts prevented oxidation in non-irradiated and gamma-irradiated fi ... | 2017 | 28764032 |
| increase in nitrite content and functionality of ethanolic extracts of perilla frutescens following treatment with atmospheric pressure plasma. | this study investigated the effect of atmospheric pressure plasma (app) treatment on nitrite content and functionality of plant extracts. ethanolic extracts of perilla frutescens (eep) were prepared and treated with app for 60min. nitrite content increased from 0 to 45.8mg/l in eep after app treatment for 60min. antimicrobial activity of eep against clostridium perfringens and salmonella typhimurium was increased by app with no influence on antioxidative activity (p<0.05). lyophilized eep (leep) ... | 2017 | 28763985 |
| salmonella typhimurium-induced il-1 release from primary human monocytes requires nlrp3 and can occur in the absence of pyroptosis. | large molecular complexes known as inflammasomes regulate the release of il-1β from immune cells in response to infection and injury. salmonella typhimurium infection is reported to activate nlrp3 and nlrc4 inflammasomes which are subsequently involved in pyroptosis of the cell and pathogen clearance. however, the response to s. typhimurium in primary human monocytes has not been studied in detail. the aim of this study was to investigate the effect of s. typhimurium on inflammasomes in primary ... | 2017 | 28761045 |
| synthetic porcine hepcidin exerts opposite roles in escherichia coli and salmonella infections. | hepcidin, an antimicrobial peptide, was discovered to integrate diverse signals from iron status and infection threat and orchestrate a series of host protective responses. there have been several studies investigating the antimicrobial role of hepcidin but controversial. here, we aim to examine the role of hepcidin in bacterial adherence and invasion in vitro we found that porcine hepcidin could decrease the amount of extracellular pathogen enterotoxigenic escherichia coli (etec) k88 that adher ... | 2017 | 28760901 |
| survival of selected foodborne pathogens on dry cured pork loins. | the safety of ready-to-eat products such as cured pork loins must be guaranteed by the food industry. in the present study, the efficacy of the dry curing process of pork loins obtained from free-range pigs in the reduction of three of the most important foodborne pathogens is analysed. a total of 28 pork loin segments, with an average weight of 0.57±0.12kg, were divided into four groups with three being inoculated by immersion with 7logcfu/ml of either salmonella typhimurium, campylobacter coli ... | 2017 | 28759797 |
| crystal structure of master biofilm regulator csgd regulatory domain reveals an atypical receiver domain. | the master regulator csgd switches planktonic growth to biofilm formation by activating synthesis of curli fimbriae and cellulose in enterobacteriaceae. csgd was classified to be the luxr response regulatory family, while its cognate sensor histidine kinase has not been identified yet. csgd consists of a c-terminal dna binding domain and an n-terminal regulatory domain that provokes the upstream signal transduction to further modulate its function. we provide the crystal structure of salmonella ... | 2017 | 28758290 |
| the phenotypic and molecular resistance induced by a single-exposure to sub-mutant prevention concentration of marbofloxacin in salmonella typhimurium isolates from swine. | in the present study, the molecular mechanisms of antibiotic resistance in salmonella typhimurium clinical isolates from pigs were investigated using a single-step mutation model of exposure to sub-mutant prevention concentrations (mpcs) of marbofloxacin. the minimum inhibitory concentrations (mics) of seven antibacterial drugs were evaluated against 30 s. typhimurium clinical isolates from different pigs. mpcs of marbofloxacin were also determined. the mechanism of marbofloxacin-resistance was ... | 2017 | 28757036 |
| green synthesized nickel nanoparticles for targeted detection and killing of s. typhimurium. | simple and sensitive colorimetric immunosensor based on peroxidase mimetic activity and photothermal effect of nickel oxide nanoparticle (niogs) has been developed to detect and kill food borne pathogen salmonella typhimurium. niogs showed superior peroxidase mimetic activity for oxidation of peroxidase substrate 3, 3', 5, 5'-tetramethylbenzidine (tmb). oxidation of tmb by niogs followed michaelis-menten kinetics with km and vmax values of 0.25mm and 2.64×10(-8)m/s respectively. niogs was coated ... | 2017 | 28755580 |
| recombinant attenuated salmonella typhimurium with heterologous expression of the salmonella choleraesuis o-polysaccharide: high immunogenicity and protection. | non-typhoidal salmonella are associated with gastrointestinal disease worldwide and invasive disease in africa. we constructed novel bivalent vaccines through the recombinant expression of heterologous o-antigens from salmonella choleraesuis in salmonella typhimurium. a recombinant asd(+) plasmid pcz1 with the cloned salmonella choleraesuis o-antigen gene cluster was introduced into three constructed salmonella typhimurium δasd mutants: slt11 (δrfbp), slt12 (δrmlb-rfbp) and slt16 (δrfbp ∆pagl::t ... | 2017 | 28754982 |
| repression of salmonella host cell invasion by aromatic small molecules from the human fecal metabolome. | the human microbiome is a collection of microorganisms that inhabit every surface of the body that is exposed to the environment, generally coexisting peacefully with their host. these microbes have important functions such as the production of vitamins, maturation of the immune system and protection against pathogens. we have previously shown that a small-molecule extract from the human fecal microbiome has a strong repressive effect on salmonella enterica serovar typhimurium host cell invasion ... | 2017 | 28754707 |
| a comparison of three peyer's patch "m-like" cell culture models: particle uptake, bacterial interaction, and epithelial histology. | intestinal peyer's patch (pp) microfold (m) cells transport microbes and particulates across the follicle-associated epithelium (fae) as part of the mucosal immune surveillance system. in vitro human m-like cell co-culture models are used as screens to investigate uptake of antigens-in-nanoparticles, but the models are labour-intensive and there is inter-laboratory variability. we compared the three most established filter-grown caco-2/raji b cell co-culture systems. these were model a (kerneis ... | 2017 | 28754262 |
| whole-genome shotgun sequences of salmonella enterica serovar typhimurium lilleengen type strains lt1, lt18, lt19, lt20, lt21, and lt22. | the lilleengen type (lt) collection of salmonella enterica serovar typhimurium strains has served the scientific community as a group of model organisms for basic genetic and biochemical pathway research. here, we report the whole-genome shotgun sequences of salmonella enterica serovar typhimurium strains lt1, lt18, lt19, lt20, lt21, and lt22. | 2017 | 28751402 |
| antibacterial electrospun chitosan-based nanofibers: a bacterial membrane perforator. | this study investigates the antibacterial action of chitosan-based nanofibers (cnfs) obtained by the electrospinning process on the permeability of bacterial membranes. the bactericidal efficiency of cnfs was first determined against gram-negative escherichia coli and salmonella typhimurium, and gram-positive staphylococcus aureus and listeria innocua bacteria as a baseline. the results strongly suggest that cnfs interact with the negatively charged bacterial cell wall causing membrane rupture a ... | 2017 | 28748074 |
| characterization of integrons and resistance genes in salmonella isolates from farm animals in shandong province, china. | a total of 154 non-duplicate salmonella isolates were recovered from 1,105 rectal swabs collected from three large-scale chicken farms (78/325, 24.0%), three large-scale duck farms (56/600, 9.3%) and three large-scale pig farms (20/180, 11.1%) between april and july 2016. seven serotypes were identified among the 154 isolates, with the most common serotype in chickens and ducks being salmonella enteritidis and in pigs salmonella typhimurium. antimicrobial susceptibility testing revealed that hig ... | 2017 | 28747906 |
| trim8 negatively regulates tlr3/4-mediated innate immune response by blocking trif-tbk1 interaction. | tlr-mediated signaling pathways play critical roles in host defense against microbials. however, dysregulation of innate immune and inflammatory responses triggered by tlrs would result in harmful damage to the host. using a trim8 gene-knockout mouse model, we show that tripartite motif (trim) 8 negatively regulates tlr3- and tlr4-mediated innate immune and inflammatory responses. trim8 deficiency leads to increased polyinosinic-polycytidylic acid- and lps-triggered induction of downstream anti- ... | 2017 | 28747347 |
| anti-adhesion and anti-biofilm potential of organosilane nanoparticles against foodborne pathogens. | nowadays, modification of surfaces by nanoparticulate coatings is a simple process that may have applications in reducing the prevalence of bacterial cells both on medical devices and food processing surfaces. to this direction, biofilm biological cycle of salmonella typhimurium, listeria monocytogenes, escherichia coli o157:h7, staphylococcus aureus, and yersinia enterocolitica on stainless steel and glass surfaces, with or without nanocoating was monitored. to achieve this, four different comm ... | 2017 | 28744277 |
| edible wild plant heracleum pyrenaicum subsp. orsinii as a potential new source of bioactive essential oils. | many heracleum l. taxa (apiaceae) are used as food and spices, and in traditional medicine. in this work, the chemical composition of heracleum pyrenaicum subsp. orsinii (guss.) f. pedrotti and pignatti root, leaf and fruit essential oils, their antimicrobial activity and cytotoxic effect on malignant and normal cells were investigated for the first time. the composition of the oils was analyzed by gc and gc-ms. monoterpenes prevailed in the root oil, with β-pinene (38.6%) being dominant, while ... | 2017 | 28740275 |
| a salmonella typhimurium outbreak linked to vietnamese bread rolls in south western sydney, australia, 2015. | in september 2015, the south western sydney (sws) public health unit was notified of a cluster of salmonella typhimurium (stm) cases with a common multiple-locus variable-number tandem repeats analysis (mlva) pattern. an investigation was conducted to identify a source and contain the outbreak. | 2017 | 28736650 |
| pathogen-induced tlr4-trif innate immune signaling in hematopoietic stem cells promotes proliferation but reduces competitive fitness. | bacterial infection leads to consumption of short-lived innate immune effector cells, which then need to be replenished from hematopoietic stem and progenitor cells (hspcs). hspcs express pattern recognition receptors, such as toll-like receptors (tlrs), and ligation of these receptors induces hspc mobilization, cytokine production, and myeloid differentiation. the underlying mechanisms involved in pathogen signal transduction in hscs and the resulting biological consequences remain poorly defin ... | 2017 | 28736216 |
| eucalyptol, sabinene and cinnamaldehyde: potent inhibitors of salmonella target protein l-asparaginase. | salmonella typhimurium is a severe threat to human life. the treatment became more difficult with the emergence of multidrug resistance. in the present in silico study, a novel drug target l-asparaginase was tested against three ligands eucalyptol, sabinene, and cinnamaldehyde, major components of cardamom, nutmeg, and cinnamon, respectively. the lowest docking score was obtained for sabinene followed by eucalyptol and cinnamaldehyde i.e. -5.648, -3.939 and -3.469. the docking score of sabinene ... | 2017 | 28735432 |
| influence of saccharomyces cerevisiae fermentation products, smartcare in milk replacer and original xpc in calf starter, on the performance and health of preweaned holstein calves challenged with salmonella enterica serotype typhimurium. | this study was designed to investigate the effects of supplementing smartcare (sc; diamond v, cedar rapids, ia) in milk replacer and original xpc (xpc; diamond v) in calf starter on performance and health of preweaned calves following an oral challenge with salmonella enterica. the study was performed in two 35-d periods with 30 holstein bull calves (2 ± 1 d of age) per period. in each period, calves were blocked by location in the barn and randomly assigned to treatments that included control, ... | 2017 | 28734601 |
| current perspectives on invasive nontyphoidal salmonella disease. | we searched pubmed for scientific literature published in the past 2 years for relevant information regarding the burden of invasive nontyphoidal salmonella disease and host factors associated with nontyphoidal salmonella infection and discuss current knowledge on vaccine development. the following search terms were used: salmonella, non typhoidal/nontyphoidal, nts, disease, bloodstream infection, invasive, sepsis/septicaemia/septicemia, bacteraemia/bacteremia, gastroenteritis, incidence, preval ... | 2017 | 28731899 |
| pen-on-paper strategy for point-of-care testing: rapid prototyping of fully written microfluidic biosensor. | paper-based microfluidic biosensors have recently attracted increasing attentions in point-of-care testing (poct) territories benefiting from their affordable, accessible and eco-friendly features, where technologies for fabricating such biosensors are preferred to be equipment free, easy-to-operate and capable of rapid prototyping. in this work, we developed a pen-on-paper (pop) strategy based on two custom-made pens, i.e., a wax pen and a conductive-ink pen, to fully write paper-based microflu ... | 2017 | 28728008 |
| development of a salmonella typhimurium challenge model in weaned pigs to evaluate effects of water and feed interventions on fecal shedding and growth performance. | the aim of this study was to develop a typhimurium (st) challenge model in weaned pigs suitable to evaluate effects of water and feed interventions on fecal shedding and growth performance. two studies were performed. in exp. 1 weaned pigs were fed either a standard diet (con) or a diet with a high buffer capacity (hb) and challenged for either 3 or 7 consecutive days in a latin square design with 4 × 8 individually housed pigs. in exp. 2, the con 7-d challenge method was chosen for further mode ... | 2017 | 28727108 |
| physiological fluid shear alters the virulence potential of invasive multidrug-resistant non-typhoidal salmonella typhimurium d23580. | salmonella enterica serovar typhimurium strains belonging to sequence type st313 are a major cause of fatal bacteremia among hiv-infected adults and children in sub-saharan africa. unlike "classical" non-typhoidal salmonella (nts), gastroenteritis is often absent during st313 infections and isolates are most commonly recovered from blood, rather than from stool. this is consistent with observations in animals, in which st313 strains displayed lower levels of intestinal colonization and higher re ... | 2016 | 28725732 |
| antimicrobial effect of black pepper petroleum ether extract for the morphology of listeria monocytogenes and salmonella typhimurium. | this study aimed to evaluate the effects of black pepper petroleum extract (bppe) on pathogenic bacteria. the extraction from black pepper showed intense antimicrobial activity against the gram-positive listeria monocytogenes atcc 19115 and the gram-negative bacteria salmonella typhimurium atcc 14028. the minimum inhibitory concentrations of bppe against l. monocytogenes and s. typhimurium were 0.625 and 1.25 mg/ml, respectively. detection of alkaline phosphatase outside the cell revealed that b ... | 2017 | 28720964 |
| lack of acrb efflux function confers loss of virulence on salmonella enterica serovar typhimurium. | acrab-tolc is the paradigm resistance-nodulation-division (rnd) multidrug resistance efflux system in gram-negative bacteria, with acrb being the pump protein in this complex. we constructed a nonfunctional acrb mutant by replacing d408, a highly conserved residue essential for proton translocation. western blotting confirmed that the acrb d408a mutant had the same native level of expression of acrb as the parental strain. the mutant had no growth deficiencies in rich or minimal medium. however, ... | 2017 | 28720734 |
| a novel pathogen capturing device for removal and detection. | a simple technique that employs an antibody coated polydimethylsiloxane tube is used for effective capturing of bloodborne and foodborne pathogens. by recirculating the entire sample through the antibody coated tube, accumulation of target pathogens is achieved, thereby delivering a higher concentration of pathogens in a small volume. the described method can provide an effective and economical solution to microbiology techniques that rely on enrichment, thereby expediting diagnostics. using thi ... | 2017 | 28717239 |
| th17-mediated cross protection against pneumococcal carriage by vaccination with a variable antigen. | serotype-specific protection against a streptococcus pneumoniae is an important limitation of the current polysaccharide-based vaccines. to prevent serotype replacement, reduce transmission and limit emergence of new variants, it is essential to induce broad protection and restrict pneumococcal colonization. in this study, we used a prototype vaccine formulation consisting of lps-detoxified outer membrane vesicles (omvs) from salmonella typhimurium displaying the variable n-terminus of pspa (α1α ... | 2017 | 28717032 |
| intestinal epithelial ecto-5' -nucleotidase (cd73) regulates intestinal colonization and infection by nontyphoidal salmonella. | ecto-5' -nucleotidase (cd73) is expressed abundantly on the apical surface of intestinal epithelial cells (iec) and functions as the terminal enzyme in the generation of extracellular adenosine. previous work has demonstrated that adenosine signaling in iecs results in a number of tissue-protective effects during inflammation; however, a rationale for its apical expression has been lacking. we hypothesized that the highly polarized expression of cd73 is indicative of an important role for extrac ... | 2017 | 28717030 |
| molecular dynamics studies on the domain swapped salmonella typhimurium survival protein sure: insights on the possible reasons for catalytic cooperativity. | stationary phase survival protein sure from salmonella typhimurium is a dimeric protein formed by the swapping of a tetramerization loop involved in the formation of a loose tetramer and a c-terminal helix. it functions as a phosphatase. the two-fold symmetry of the dimeric protein was lost in the mutants h234a and d230a/h234a in which a crucial hydrogen bond in the hinge involved in c-terminal helix swapping was eliminated. the catalytic activity of both mutants was drastically reduced. in cont ... | 2017 | 28714824 |
| quantification of salmonella survival and infection in an in vitro model of the human intestinal tract as proxy for foodborne pathogens. | different techniques are available for assessing differences in virulence of bacterial foodborne pathogens. the use of animal models or human volunteers is not expedient for various reasons; the use of epidemiological data is often hampered by lack of crucial data. in this paper, we describe a static, sequential gastrointestinal tract (git) model system in which foodborne pathogens are exposed to simulated gastric and intestinal contents of the human digestive tract, including the interaction of ... | 2017 | 28713334 |
| vasoactive intestinal polypeptide and mast cells regulate increased passage of colonic bacteria in patients with irritable bowel syndrome. | irritable bowel syndrome (ibs) is associated with intestinal dysbiosis and symptoms of ibs develop following gastroenteritis. we aimed to study passage of live bacteria through the colonic epithelium, and determine the role of mast cells and vasoactive intestinal polypeptide (vip) in barrier regulation in ibs and healthy individuals. | 2017 | 28711627 |
| characterization and bioactivities of the exopolysaccharide from a probiotic strain of lactobacillus plantarum wlpl04. | exopolysaccharide (eps) was extracted and purified from lactobacillus plantarum wlpl04, which has been confirmed previously as a potential probiotic for its antagonistic and immune-modulating activity. it has a molecular weight of 6.61 × 10(4) da, consisting of xylose, glucose, and galactose in an approximate molar ratio of 3.4: 1.8: 1. microstructural studies demonstrated that the eps appeared as a smooth sheet structure with many homogeneous rod-shaped lumps. the preliminary in vitro assays in ... | 2017 | 28711240 |
| modeling polymorphic transformation of rotating bacterial flagella in a viscous fluid. | the helical flagella that are attached to the cell body of bacteria such as escherichia coli and salmonella typhimurium allow the cell to swim in a fluid environment. these flagella are capable of polymorphic transformation in that they take on various helical shapes that differ in helical pitch, radius, and chirality. we present a mathematical model of a single flagellum described by kirchhoff rod theory that is immersed in a fluid governed by stokes equations. we perform numerical simulations ... | 2017 | 28709256 |
| growth characterization of single and double salmonella methionine auxotroph strains for potential vaccine use in poultry. | poultry meat is an important source of zoonotic salmonella infection. oral vaccination of chickens with live attenuated salmonella during grow-out is an attractive approach to control salmonella colonization in the chicken gastrointestinal tract. in this study, we report the construction of methionine-dependent and growth of salmonella typhimurium mutant strains with methionine auxotrophy (δmetr and δδmetrmetd) and survival in chicken feed and fecal matrices. the methionine auxotroph mutant δδme ... | 2017 | 28706896 |
| salmonella typhimurium infection in total knee arthroplasty: a case report with review of literature. | salmonella enterica serotype typhimurium is a rare cause of prosthetic joint infection (pji). the recognized predisposing risk factors for salmonella septic arthritis include diabetes mellitus, renal failure, human immunodeficiency virus infection and chronic corticosteroid use. we describe a case of pji of the knee in a 74-year-old lady who was on antitubercular treatment. the patient presented with discharging sinus and raised inflammatory markers. she was successfully treated by the removal o ... | 2017 | 28706395 |
| development and characterization of bioactive edible films from spider crab (maja crispata) chitosan incorporated with spirulina extract. | active food packaging films based on crab chitosan and spirulina extract (se) were developed. the effects of the se incorporation at different levels on physical (color, opacity water vapor and oxygen permeability) and mechanical (tensile strength and elongation at break) properties of chitosan films were investigated. ftir was carried out to observe the potential modifications of the chitosan films when incorporated with se. the obtained results suggested that incorporation of se into chitosan ... | 2017 | 28705500 |
| quinolones and fluroquinolones metabolism and resistance in salmonella typhimurium: a mini review. | salmonella enterica serovars causes substantial morbidity and mortality worldwide. more than 2500 different serotypes of salmonella were identified to date. in particular, salmonella enterica serovar typhimurium is the most important serotype of salmonella transmitted from animals to humans in most parts of the world. they are associated with an estimated 1 million deaths annually. quinolones are an important class of broad-spectrum antimicrobials has been utilized in the treatment option for ov ... | 2017 | 28699507 |
| antimutagenic and antioxidant activities of thai rice brans. | rice bran is the outer layer of the rice grain, and contains high amounts of bioactive phytochemicals. here, we investigated and compared chemopreventive properties of purple and white rice bran extracts. | 2017 | 28698862 |
| salmonella enterica serovar typhimurium st34 co-expressing blandm-5 and blactx-m-55 isolated in china. | 2017 | 28698668 | |
| investigation of medium chain fatty acid feed supplementation for reducing salmonella typhimurium colonization in turkey poults. | studies indicate that persistent salmonella colonization occurs in poultry that are infected early in life, leading to both food safety and public health concerns. development of improved preharvest salmonella management strategies is needed to reduce poultry product contamination. the objective of this study was to evaluate the efficacy of a product containing medium chain fatty acids (mcfa) for reducing early salmonella colonization in turkey poults. day-of-hatch turkeys were provided a standa ... | 2017 | 28696788 |
| development of a novel, rapid multiplex polymerase chain reaction assay for the detection and differentiation of salmonella enterica serovars enteritidis and typhimurium using ultra-fast convection polymerase chain reaction. | salmonella enterica serovars enteritidis and typhimurium are the most common causative agents of human nontyphoidal salmonellosis. the rapid detection and timely treatment of salmonellosis are important to increase the curative ratio and prevent spreading of the disease. in this study, we developed a rapid multiplex convection polymerase chain reaction (pcr) method to detect salmonella spp. and differentiate salmonella enteritidis and salmonella typhimurium. we used the inva gene for salmonella ... | 2017 | 28696782 |
| seeing but not believing: the structure of glycerol dehydrogenase initially assumed to be the structure of a survival protein from salmonella typhimurium. | the determination of the crystal structure of a mutant protein using phases based on a previously determined crystal structure of the wild-type protein is often a straightforward molecular-replacement protocol. such a structure determination may be difficult if there are large-scale structural differences between the wild-type and mutant proteins. in this manuscript, an interesting case is presented of the unintentional crystallization of a contaminant protein which shared some structural featur ... | 2017 | 28695861 |
| genotoxic and cytotoxic potential of whole plant extracts of kalanchoe laciniata by ames and mtt assay. | lack of data on safety of herbal medicines have endangered human health and life. the present study evaluated the genotoxic and mutagenic effect of kalanchoe laciniata to access the safety and usefulness of the medicinal plant. aqua-methanolic and n-hexane extracts of k. laciniata were evaluated for the genotoxic potential using ames assay and cytotoxicity was evaluated using mtt assay. ames assay was conducted using two strains of salmonella typhimurium ta-100 and ta-102 whereas mtt assay was p ... | 2017 | 28694760 |
| structural studies on the decameric s. typhimurium arginine decarboxylase (adc): pyridoxal 5'-phosphate binding induces conformational changes. | enteric pathogens such as salmonella typhimurium colonize the human gut in spite of the lethal acidic ph environment (ph < 2.5) due to the activation of inducible acid tolerance response (atr) systems. the pyridoxal 5'-phosphate (plp)-dependent enzyme, biodegradative arginine decarboxylase (adc, encoded by adia), is a component of an atr system. the enzyme consumes a cytoplasmic proton in the process of arginine degradation to agmatine. arginine-agmatine antiporter (adic) exchanges the product a ... | 2017 | 28694189 |
| population-based incidence, seasonality and serotype distribution of invasive salmonellosis among children in nanoro, rural burkina faso. | bloodstream infections (bsi) caused by salmonella typhi and invasive non-typhoidal salmonella (ints) frequently affect children living in rural sub-saharan africa but data about incidence and serotype distribution are rare. | 2017 | 28692655 |
| role of the inner-membrane histidine kinase rcsc and outer-membrane lipoprotein rcsf in the activation of the rcs phosphorelay signal transduction system in escherichia coli. | the rcs phosphorelay signal transduction system of escherichia coli controls genes for capsule production and many other envelope-related functions and is implicated in biofilm formation. the outer-membrane lipoprotein rcsf is an essential component of the rcs system. mislocalization of rcsf to the periplasm or the cytoplasmic membrane leads to high activation of the rcs system, suggesting that rcsf functions by interacting with the cytoplasmic membrane component(s) of the system in activating t ... | 2017 | 28691662 |
| culling of apcs by inflammatory cell death pathways restricts tim3 and pd-1 expression and promotes the survival of primed cd8 t cells. | we evaluated the impact of premature cell death of antigen-presenting cells (apcs) by caspase-1- and ripk3-signaling pathways on cd8(+) t-cell priming during infection of mice with salmonella typhimurium (st). our results indicate that caspase1 and ripk3 synergize to rapidly eliminate infected apcs, which does not influence the initial activation of cd8(+) t cells. however, the maintenance of primed cd8(+) t cells was greatly compromised when both these pathways were disabled. caspase-1- and rip ... | 2017 | 28686578 |
| selective and high dynamic range assay format for multiplex detection of pathogenic pseudomonas aeruginosa, salmonella typhimurium, and legionella pneumophila rnas using surface plasmon resonance imaging. | due to its well-characterized and highly conserved structure, as well as its relative abundance in metabolically active cells, bacterial 16s rrna sequence plays an important role in microbial identification. in this work, a biosensing strategy has been developed for simultaneous detection of 16s rrna analytes of three pathogenic bacterial strains: legionella pneumophila, pseudomonas aeruginosa, and salmonella typhimurium. surface plasmon resonance imaging (spri) was used as a detection technique ... | 2017 | 28682593 |
| mesenchymal cell-specific myd88 signaling promotes systemic dissemination of salmonella typhimurium via inflammatory monocytes. | enteric pathogens including salmonella enteric serovar typhimurium can breach the epithelial barrier of the host and spread to systemic tissues. in response to infection, the host activates innate immune receptors via the signaling molecule myd88, which induces protective inflammatory and antimicrobial responses. most of these innate immune responses have been studied in hematopoietic cells, but the role of myd88 signaling in other cell types remains poorly understood. surprisingly, we found tha ... | 2017 | 28674182 |
| temporal regulation of a salmonella typhimurium virulence factor by the transcriptional regulator ydcr. | we previously examined salmonella proteome within infected host cells and found differential expression of many proteins with defined functional roles such as metabolism or virulence. however, the precise roles of other altered proteins in salmonella pathogenesis are largely unknown. a putative transcriptional regulator, ydcr, was highly induced intracellularly while barely expressed in vitro, implicating potential relevance to bacterial infection. to unveil its physiological functions, we explo ... | 2017 | 28674150 |
| role of sapa and yfga in susceptibility to antibody-mediated complement-dependent killing and virulence of salmonella enterica serovar typhimurium. | the st313 pathovar of salmonella enterica serovar typhimurium contributes to a high burden of invasive disease among african infants and hiv-infected adults. it is characterized by genome degradation (loss of coding capacity) and has increased resistance to antibody-dependent complement-mediated killing compared with enterocolitis-causing strains of s typhimurium. vaccination is an attractive disease-prevention strategy, and leading candidates focus on the induction of bactericidal antibodies. a ... | 2017 | 28674031 |