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comparative genomics of australian and international isolates of salmonella typhimurium: correlation of core genome evolution with crispr and prophage profiles.salmonella enterica subsp enterica serovar typhimurium (s. typhimurium) is a serovar with broad host range. to determine the genomic diversity of s. typhimurium, we sequenced 39 isolates (37 australian and 2 uk isolates) representing 14 repeats groups (rgs) determined primarily by clustered regularly interspaced short palindromic repeats (crispr). analysis of single nucleotide polymorphisms (snps) among the 39 isolates yielded an average of 1,232 snps per isolate, ranging from 128 snps to 11,339 ...201728851865
whole-genome sequences of salmonella enterica subsp. enterica serovar typhimurium strains tt6675 and tt9097 employed in the isolation and characterization of a giant phage mutant collection.we report here the genome sequences of salmonella enterica subsp. enterica serovar typhimurium strains tt6675 and tt9097, which we utilize for genetic analyses of giant bacterial viruses. our analyses identified several genetic variations between the two strains, most significantly confirming strain tt6675 as a serine suppressor and tt9097 as a nonsuppressor.201728839032
use of multiple-locus variable-number of tandem repeats analysis (mlva) to investigate genetic diversity of salmonella enterica subsp. enterica serovar typhimurium isolates from human, food, and veterinary sources.salmonella enterica subspecies enterica serovar typhimurium is the most common zoonotic pathogen in bulgaria. to allow efficient outbreak investigations and surveillance in the food chain, accurate and discriminatory methods for typing are needed. this study evaluated the use of multiple-locus variable-number of tandem repeats analysis (mlva) and compared results with antimicrobial resistance (amr) determinations for 100 s. typhimurium strains isolated in bulgaria during 2008-2012 (50 veterinary ...201728836358
genome sequence of an australian monophasic salmonella enterica subsp. enterica typhimurium isolate (tw-stm6) carrying a large plasmid with multiple antimicrobial resistance genes.we report the genome sequence of a monophasic salmonella enterica subsp. enterica typhimurium strain (tw-stm6) isolated in australia that is similar to epidemic multidrug-resistant strains from europe and elsewhere. this strain carries additional antibiotic and heavy-metal resistance genes on a large (275-kb) inchi2 plasmid.201728818900
ground water as the source of an outbreak of salmonella enteritidis.in september 2014, an outbreak of gastroenteritis was reported to the public health institute of šibenik and knin county in croatia. the outbreak occurred in the county center of šibenik, a town with 50,000 inhabitants, and it lasted for 12days. an epidemiological investigation suggested a nearby water spring as the source of the outbreak. due to the temporary closure of the public water supply system, the inhabitants started to use untreated water from a nearby spring. microbiological analysis ...201728756827
quinolone susceptibility and genetic characterization of salmonella enterica subsp. enterica isolated from pet turtles.turtle-borne salmonella enterica owns significance as a leading cause in human salmonellosis. the current study aimed to determine the quinolone susceptibility and the genetic characteristics of 21 strains of s. enterica subsp. enterica isolated from pet turtles. susceptibility of four antimicrobials including nalidixic acid, ciprofloxacin, ofloxacin, and levofloxacin was examined in disk diffusion and mic tests where the majority of the isolates were susceptible to all tested quinolones. in gen ...201728747968
draft genome sequence of salmonella enterica subsp. enterica serovar infantis strain spe101, isolated from a chronic human infection.we report a 4.99-mb draft genome sequence of salmonella enterica subsp. enterica serovar infantis strain spe101, isolated from feces of a 5-month-old breast-fed female showing diarrhea associated with severe dehydration and malnutrition. the infection prolonged for 6 months despite antibiotic treatment.201728729277
draft genome sequences of 256 salmonella enterica subsp. enterica serovar enteritidis strains isolated from humans, food, chickens, and farm environments in brazil.salmonella enterica subsp. enterica serovar enteritidis emerged in the late 1980s as the most isolated salmonella serovar worldwide. here, we report the draft genomes of 256 s enteritidis strains isolated from humans, food, chickens, and farm environments in brazil. these draft genomes will help enhance our understanding of this serovar in brazil.201728705959
relationship of triamine-biocide tolerance of salmonella enterica serovar senftenberg to antimicrobial susceptibility, serum resistance and outer membrane proteins.a new emerging phenomenon is the association between the incorrect use of biocides in the process of disinfection in farms and the emergence of cross-resistance in salmonella populations. adaptation of the microorganisms to the sub-inhibitory concentrations of the disinfectants is not clear, but may result in an increase of sensitivity or resistance to antibiotics, depending on the biocide used and the challenged salmonella serovar. exposure of five salmonella enterica subsp. enterica serovar se ...201728696348
complete genome sequences of two salmonella enterica subsp. enterica serovar enteritidis strains isolated from egg products in the united states.egg-associated salmonellosis is an important public health problem in many countries. here, we report the genome sequences, including plasmids, of two strains of salmonella enterica subsp. enterica serovar enteritidis isolated from egg products in 2012 and 2013 in the united states. this will provide more information and insight into the research about egg-associated salmonellosis.201728663302
in vitro and in vivo generation of heterophil extracellular traps after salmonella exposure.the release of extracellular traps (ets) by granulocytes is a unique strategy to stop the dissemination of microbial pathogens. this study was undertaken to elucidate the potential of avian granulocytes (heterophils) to form ets that can arrest and kill salmonella organisms. after in vitro exposure of isolated heterophils and in vivo infection of day-old chicks with salmonella enterica subsp. enterica serovars infantis (si) or enteritidis (se), the generation of ets as well as the trapping and s ...201728615122
in vitro characterization of lactobacillus plantarum strains with inhibitory activity on enteropathogens for use as potential animal probiotics.the present study evaluates the probiotic properties of three lactobacillus plantarum strains mjm60319, mjm60298, and mjm60399 possessing antimicrobial activity against animal enteric pathogens. the three strains did not show bioamine production, mucinolytic and hemolytic activity and were susceptible to common antibiotics. the l. plantarum strains survived well in the simulated orogastrointestinal transit condition and showed adherence to caco-2 cells in vitro. the l. plantarum strains showed s ...201728611498
a role for the tetraspanin proteins in salmonella infection of human macrophages.infected macrophages play a role in the dissemination of salmonella and may serve as a reservoir of infection in asymptomatic carriers. however, relatively little is known about the early molecular interactions of the bacteria with these cells. we have recently shown that members of the tetraspanin family of membrane proteins are involved in the initial adhesion of a range of bacteria to host cells. this study investigated the role of tetraspanins in salmonella enterica subsp. enterica serovar t ...201728602860
molecular subtyping of salmonella typhimurium with multiplex oligonucleotide ligation-pcr (mol-pcr).a multiplex oligonucleotide ligation-pcr (mol-pcr) assay is a valuable high-throughput technique for the detection of bacteria and viruses, for characterization of pathogens and for diagnosis of genetic diseases, as it allows one to combine different types of molecular markers in a high-throughput multiplex assay. a mol-pcr assay starts with a multiplex oligonucleotide ligation reaction for detection of the molecular marker, followed by a singleplex pcr for signal amplification and analysis of t ...201728600761
histopathology case definition of naturally acquired salmonella enterica serovar dublin infection in young holstein cattle in the northeastern united states.salmonella enterica subsp. enterica serovar dublin ( salmonella dublin) is a host-adapted bacterium that causes high morbidity and mortality in dairy cattle worldwide. a retrospective search of archives at the new york animal health diagnostic center revealed 57 culture-confirmed salmonella dublin cases from new york and pennsylvania in which detailed histology of multiple tissues was available. tissues routinely submitted by referring veterinarians for histologic evaluation included sections of ...201728599615
what were the risk factors and trends in antimicrobial resistance for enteric fever in london 2005-2012?a study was undertaken to determine the risk factors and trends in antimicrobial resistance for enteric fever.201728590238
next generation sequencing-based multigene panel for high throughput detection of food-borne pathogens.contamination of food by chemicals or pathogenic bacteria may cause particular illnesses that are linked to food consumption, commonly referred to as foodborne diseases. bacteria are present in/on various foods products, such as fruits, vegetables and ready-to-eat products. bacteria that cause foodborne diseases are known as foodborne pathogens (fbps). accurate detection methods that are able to reveal the presence of fbps in food matrices are in constant demand, in order to ensure safe foods wi ...201728578266
quantitative risk assessment of antimicrobial-resistant foodborne infections in humans due to recombinant bovine somatotropin usage in dairy cows.recombinant bovine somatotropin (rbst) is a production-enhancing technology that allows the dairy industry to produce milk more efficiently. concern has been raised that cows supplemented with rbst are at an increased risk of developing clinical mastitis, which would potentially increase the use of antimicrobial agents and increase human illnesses associated with antimicrobial-resistant bacterial pathogens delivered through the dairy beef supply. the purpose of this study was to conduct a quanti ...201728574304
complete genome sequences of three salmonella enterica subsp. enterica serovar saintpaul isolates associated with a 2013 multistate outbreak in the united states.in 2013, a multistate outbreak of salmonella enterica subsp. enterica serovar saintpaul from cucumber caused 84 cases of salmonellosis in the united states. in this announcement, we report the complete genome sequences of three clinical salmonella saintpaul isolates associated with the 2013 outbreak.201728572327
susceptibility of salmonella biofilm and planktonic bacteria to common disinfectant agents used in poultry processing.poultry contaminated with salmonella enterica subsp. enterica are a major cause of zoonotic foodborne gastroenteritis. salmonella heidelberg is a common serotype of salmonella that has been implicated as a foodborne pathogen associated with the consumption of improperly prepared chicken. to better understand the effectiveness of common antimicrobial disinfectants (i.e., peroxyacetic acid [paa], acidified hypochlorite [ach], and cetylpyridinium chloride [cpc]), environmental isolates of nontyphoi ...201728561639
mitigation of salmonella on pet food kibbles by using liquid and powdered 3-hydroxy-3-methylbutyric acid.in recent years, several pet food recalls have been attributed to salmonella contamination. in addition to the negative impacts on animal health, salmonella-contaminated pet foods have been linked to infection in humans. with that in mind, the u.s. food and drug administration has set forth a zero-tolerance policy for salmonella in pet foods. typically, pet foods are extruded or processed at high temperatures that are sufficient to reduce pathogenic bacteria. however, the possibility for postext ...201728561638
ca(2+) in hybridization solutions for fluorescence in situ hybridization facilitates the detection of enterobacteriaceae.fluorescence in situ hybridization (fish) has been employed to identify microorganisms at the single cell level under a microscope. extensive efforts have been made to improve and extend the fish technique; however, the development of a widely applicable protocol is a continuing challenge. the present study evaluated the effects of divalent cations in the hybridization solution on the fish-based detection of various species of bacteria and archaea with rrna-targeted probes. a flow cytometric ana ...201728515389
temporal genomic phylogeny reconstruction indicates a geospatial transmission path of salmonella cerro in the united states and a clade-specific loss of hydrogen sulfide production.salmonella cerro has become one of the most prevalent salmonella serotypes isolated from dairy cattle in several u.s. states, including new york where it represented 36% of all salmonella isolates of bovine origin in 2015. this serotype is commonly isolated from dairy cattle with clinical signs of salmonellosis, including diarrhea and fever, although it has also been identified in herds without evidence of clinical disease or decreased production. to better understand the transmission patterns a ...201728507536
emergence of multidrug resistance in locally-acquired human infections with salmonella typhimurium in australia owing to a new clade harbouring blactx-m-9.antimicrobial resistance in non-typhoidal salmonella is a critical problem globally, with the emergence of resistance to third-generation cephalosporins (3gcs) a particular concern. the aim of this study was to use whole-genome sequencing (wgs) to characterise recently identified human and non-human isolates of 3gc-resistant salmonella enterica subsp. enterica serovar typhimurium from australia. the illumina nextseq sequencing platform was used to determine the genome sequences of 78 s. typhimur ...201728476613
influence of static magnetic field exposure on fatty acid composition in salmonella hadar.we have been interested, in this work, to investigate the effect of the exposure to static magnetic field at 200 mt (smf) on the fatty acid (fa) composition of salmonella enterica subsp enterica serovar hadar isolate 287: effects on the proportion of saturated and unsaturated fatty acids (sfas, ufas), cyclopropane fatty acids (cfas) and hydroxy fatty acids after exposure to the static magnetic field at 200 mt (smf). analysis with gas chromatography-mass spectrometry (gc-ms) of total lipid showed ...201728455137
draft genome sequences of salmonella enterica subsp. enterica serovar typhimurium strains isolated from chicken and swine carcasses in two distinct geographical regions from rio de janeiro state, brazil.salmonella enterica subsp. enterica serovar typhimurium is a surveyed worldwide serotype with well-characterized genomes for several different strains. in brazil, very few studies have submitted whole-genome sequences to genbank. this genome may be useful to analyze the genetic mechanisms comparable to those of other related studies conducted in brazil and globally.201728450502
sensitivity to antibiotics of bacteria exposed to gamma radiation emitted from hot soils of the high background radiation areas of ramsar, northern iran.over the past several years our laboratories have investigated different aspects of the challenging issue of the alterations in bacterial susceptibility to antibiotics induced by physical stresses.201728432369
genotypic relatedness and antimicrobial resistance of salmonella heidelberg isolated from chickens and turkeys in the midwestern united states.salmonella is one of the most common causes of foodborne illnesses in humans in the united states, and domestic poultry is considered an important source of this pathogen. salmonella enterica subsp. enterica serovar heidelberg is the fourth most commonly reported salmonella from retail meats and food animals in the united states. we assessed the genotypes and antimicrobial resistance phenotypes of salmonella heidelberg isolated from various chicken and turkey hatcheries and breeder farms in the ...201728430086
safety, protective immunity, and diva capability of a rough mutant salmonella pullorum vaccine candidate in broilers.salmonella enterica subsp. enterica serovar gallinarum biovar pullorum (salmonella pullorum) is highly adapted to chickens causing an acute systemic disease that results in high mortality. vaccination represents one approach for promoting animal health, food safety and reducing environmental persistence in salmonella control. an important consideration is that salmonella vaccination in poultry should not interfere with the salmonellosis monitoring program. this is the basis of the diva (differen ...201728424675
dynamics of mono- and dual-species biofilm formation and interactions between staphylococcus aureus and gram-negative bacteria.microorganisms are not commonly found in the planktonic state but predominantly form dual- and multispecies biofilms in almost all natural environments. bacteria in multispecies biofilms cooperate, compete or have neutral interactions according to the involved species. here, the development of mono- and dual-species biofilms formed by staphylococcus aureus and other foodborne pathogens such as salmonella enterica subsp. enterica serovar enteritidis, potentially pathogenic raoultella planticola a ...201728401747
susceptibility of salmonella enterica isolates from tomato farm environments to fatty acids naturally found on tomato fruit.salmonella enterica subsp. enterica can colonize tomato fruit as it interacts with fruit surface compounds. the exometabolome of tomato fruit contains a mixture of compounds, including fatty acids, which could affect salmonella fitness. fatty acids detected in fruit exudates were investigated for salmonella inhibition. pelargonic, lauric, myristic, palmitic, margaric, stearic, and oleic acids were suspended in water dissolved in dimethyl sulfoxide (dmso) or emulsified in water and quillaja sapon ...201728398868
whole-genome comparative analysis of salmonella enterica serovar newport strains reveals lineage-specific divergence.salmonella enterica subsp. enterica serovar newport has been associated with various foodborne outbreaks in humans and animals. phylogenetically, serovar newport is one of several salmonella serovars that are polyphyletic. to understand more about the polyphyletic nature of this serovar, six food, environment, and human isolates from different newport lineages were selected for genome comparison analyses. whole genome comparisons demonstrated that heterogeneity mostly occurred in the prophage re ...201728379364
anti-salmonella activity and probiotic trends of kluyveromyces marxianus s-2-05 and kluyveromyces lactis s-3-05 isolated from a french cheese, tomme d'orchies.kluyveromyces marxianus s-2-05 and kluyveromyces lactis s-3-05 were recently isolated from a traditional french cheese, tomme d'orchies, and characterized here for their advantages using a different application perspective. first, we established their anti-salmonella activity and downregulation of the virulence sopd gene of salmonella enterica subsp. enterica serovar typhimurium, mainly in the presence of k. marxianus s-2-05. in addition to their antagonism, these non-saccharomyces yeasts were a ...201728366839
detection of brucellosis in sika deer ( cervus nippon ) through loop-mediated isothermal amplification (lamp).brucellosis (brucella bovis) in sika deer ( cervus nippon ) can cause enormous losses to stag breeding, especially in areas in which stag breeding has become an important industry. it also poses a threat to humans because it is a zoonotic disease. use of the loop-mediated isothermal amplification (lamp) assay has been poorly described in the diagnosis of brucellosis in deer. we developed a lamp assay targeting the omp25 gene sequence to detect brucellosis (brucella bovis) in sika deer. the react ...201728318383
comparative subproteomic analysis of clinically acquired fluoroquinolone resistance and ciprofloxacin stress in salmonella typhimurium dt104b.antimicrobial resistance is a worldwide public health threat and salmonella enterica subsp. enterica serotype typhimurium phage type dt104b multiresistant strains with additional quinolone resistance have been responsible for global outbreaks and high mortality. quinolone resistance is known to be multifactorial but is still far from a complete understanding. to give new insights about the resistance mechanisms involved, this work aimed to evaluate subproteome changes between a s. typhimurium dt ...201728314077
complete genome and methylome sequences of salmonella enterica subsp. enterica serovars typhimurium, saintpaul, and stanleyville from the sara/sarb collection.in this announcement, we report the complete genome and methylome sequences of three salmonella enterica strains from the sara and sarb collection: s. enterica subsp. enterica serovar typhimurium (sara13), s. enterica subsp. enterica serovar saintpaul (sara26), and s. enterica subsp. enterica serovar stanleyville (sarb61).201728302778
the expanding targetome of small rnas in salmonella typhimurium.the enterobacterial pathogen salmonella has long served as a model for bacterial pathogenesis, stress response, gene expression and regulation with extensive investigation involving protein function. with the advent of high-throughput sequencing technologies and their applications in genomics and transcriptomics, a wealth of data particularly with respect to small rnas (srnas) is being generated. these molecules serve as regulators of major stress response and virulence networks in diverse speci ...201728302471
kidney allograft pyelonephritis caused by salmonella enterica serovar schwarzengrund.kidney transplant recipients (ktrs) taking immunosuppressive drugs have a 20-fold greater risk of nontyphoidal salmonella (nts) infection than the healthy adult population. among ktrs, the urinary tract is the most common site of infection. however, few cases of urinary tract infection caused by nts have been documented in ktrs, and only one in japan. furthermore, it frequently induces acute allograft rejection with high mortality. salmonella enterica subsp. enterica serovar schwarzengrund (s. s ...201728302440
genome sequence of the thermotolerant foodborne pathogen salmonella enterica serovar senftenberg atcc 43845 and phylogenetic analysis of loci encoding increased protein quality control mechanisms.salmonella enterica subsp. enterica bacteria are important foodborne pathogens with major economic impact. some isolates exhibit increased heat tolerance, a concern for food safety. analysis of a finished-quality genome sequence of an isolate commonly used in heat resistance studies, s. enterica subsp. enterica serovar senftenberg 775w (atcc 43845), demonstrated an interesting observation that this strain contains not just one, but two horizontally acquired thermotolerance locus homologs. these ...201728293682
mlva for salmonella enterica subsp. enterica serovar dublin: development of a method suitable for inter-laboratory surveillance and application in the context of a raw milk cheese outbreak in france in 2012.salmonella enterica subspecies enterica serovar dublin (s. dublin) figures among the most frequently isolated salmonella strains in humans in france. this serovar may affect production and animal health mainly in cattle herds with corresponding high economic losses. given that the current gold standard method, pulsed-field gel electrophoresis (pfge), provides insufficient discrimination for epidemiological investigations, we propose a standard operating procedure in this study for multiple-locus ...201728289408
draft genome sequences of 18 salmonella enterica subsp. enterica serovar oranienburg strains isolated from rivers in northwestern mexico.salmonella enterica subsp. enterica serovar oranienburg is recognized as a foodborne pathogen widely distributed in the environment. here, we report 18 draft genomes of s oranienburg strains isolated from rivers in the northwestern region of mexico.201728280020
genome sequences of salmonella enterica subsp. enterica serovar infantis strains from hungary representing two peak incidence periods in three decades.four strains of salmonella enterica subsp. enterica serovar infantis isolated from humans (1980 to 1982) and broiler chickens (2016) have been sequenced. they represent the early and recent peak incidences of this serovar in hungary. genome sequences of these isolates provide comparative data on the evolution and rise of an endemic s infantis clone in hungary.201728254986
effect of temperature and nutrient concentration on survival of foodborne pathogens in deciduous fruit processing environments for effective hygiene management.temperature and good sanitation practices are important factors for controlling growth of microorganisms. fresh produce is stored at various temperatures to ensure quality and to prolong shelf life. when foodborne pathogens survive and grow on fresh produce at storage temperatures, then additional control strategies are needed to inactivate these pathogens. the aim of this study was to determine how temperatures associated with deciduous fruit processing and storage facilities (0.5, 4, and 21°c) ...201628221909
development and validation of a cultural method for the detection and isolation of salmonella in cloves.detection of salmonella in some spices, such as cloves, remains a challenge due to their inherent antimicrobial properties. the purpose of this study was to develop an effective detection method for salmonella from spices using cloves as a model. two clove varieties, ceylon and madagascar, were used in the study. cloves were inoculated with salmonella enterica subsp. enterica serotypes montevideo, typhimurium, or weltevreden at about 1, 3, or 6 log cfu/25 g. two test portion sizes, 10 and 25 g, ...201728199150
whole-genome sequence and annotation of salmonella enterica subsp. enterica serovar enteritidis phage type 8 strain en1660.the genome of salmonella enterica subspecies enterica serovar enteritidis phage type 8 strain en1660, isolated from an outbreak in thunder bay, canada, was sequenced to 46-fold coverage using an illumina miseq with 300-bp paired-end sequencing chemistry to produce 28 contigs with an n50 value of 490,721 bp.201728126943
rapid concentration detection and differentiation of bacteria in skimmed milk using surface enhanced raman scattering mapping on 4-mercaptophenylboronic acid functionalized silver dendrites.a novel method was developed to rapidly concentrate, detect, and differentiate bacteria in skimmed milk using surface enhanced raman scattering (sers) mapping on 4-mercaptophenylboronic acid (4-mpba) functionalized silver (ag) dendrites. the 4-mpba functionalized ag dendritic sers substrate was used to capture the bacterial cells and enhance the bacterial signal. salmonella, a significantly important food pathogen, was used as the representative strain to optimize and evaluate the developed meth ...201728091716
collateral damage: insights into bacterial mechanisms that predispose host cells to cancer.infections are estimated to contribute to 20% of all human tumours. viruses are known to induce cell transformation, but evidence has also linked bacteria, such as helicobacter pylori and salmonella enterica subsp. enterica serovar typhi, to different cancer types. in addition, chlamydia trachomatis, fusobacterium nucleatum and bacteroides fragilis are associated with the development of cancer, although a causal relationship has not yet been established. bacterial effectors such as colibactin an ...201728045107
fowl typhoid in laying hens cause hepatic oxidative stress.the aim of this study was to analyses nitric oxide, antioxidant status, and oxidative profile in the liver of laying hens naturally infected by salmonella enterica subsp enterica serovar gallinarum (s. gallinarum). the nitrite/nitrate (nox), reactive oxygen species (ros), thiobarbituric acid-reactive substances (tbars), catalase (cat), glutathione reductase (gr), glutathione peroxidase (gpx) and glutathione s-transferase (gst) activities were measured in liver samples, as well the biomarkers of ...201728027943
draft genome sequence of a multidrug-resistant cmy-2-producing salmonella enterica subsp. enterica serovar minnesota st3088 isolated from chicken meat. 201728017672
evaluation of molecular typing of foodborne pathogens in european reference laboratories from 2012 to 2013.in 2012, the european centre for disease prevention and control (ecdc) initiated external quality assessment (eqa) schemes for molecular typing including the national public health reference laboratories in europe. the overall aim for these eqa schemes was to enhance the european surveillance of food-borne pathogens by evaluating and improving the quality and comparability of molecular typing. the eqas were organised by statens serum institut (ssi) and included salmonella enterica subsp. enteric ...201628006653
genome sequences of multidrug-resistant salmonella enterica subsp. enterica serovar infantis strains from broiler chicks in hungary.three strains of salmonella enterica serovar infantis isolated from healthy broiler chickens from 2012 to 2013 have been sequenced. comparison of these and previously published s infantis genome sequences of broiler origin in 1996 and 2004 will provide new insight into the genome evolution and recent spread of s infantis in poultry.201627979950
evaluation of ompa and pgte genes in determining pathogenicity in salmonella enterica serovar enteritidis.salmonella enterica subsp. enterica serovar enteritidis (s. enteritidis) is a major causative agent of gastroenteritis in humans. this important food-borne pathogen also colonises the intestinal tracts of poultry and can spread systemically, especially in chickens. to identify the s. enteritidis virulence genes involved in infection and colonisation of chickens, chromosomal deletion mutants of the ompa and pgte genes, which encode essential components of omptins, were constructed. there were no ...201627938704
metagenomics of two severe foodborne outbreaks provides diagnostic signatures and signs of coinfection not attainable by traditional methods.diagnostic testing for foodborne pathogens relies on culture-based techniques that are not rapid enough for real-time disease surveillance and do not give a quantitative picture of pathogen abundance or the response of the natural microbiome. powerful sequence-based culture-independent approaches, such as shotgun metagenomics, could sidestep these limitations and potentially reveal a pathogen-specific signature on the microbiome that would have implications not only for diagnostics but also for ...201727881416
is the evolution of salmonella enterica subsp. enterica linked to restriction-modification systems?salmonella enterica subsp. enterica bacteria are highly diverse foodborne pathogens that are subdivided into more than 1,500 serovars. the diversity is believed to result from mutational evolution, as well as intra- and interspecies recombination that potentially could be influenced by restriction-modification (rm) systems. the aim of this study was to investigate whether rm systems were linked to the evolution of salmonella enterica subsp. enterica. the study included 221 salmonella enterica ge ...201727822532
complete whole-genome sequence of salmonella enterica subsp. enterica serovar java nctc5706.salmonellae are a significant cause of morbidity and mortality globally. here, we report the first complete genome sequence for salmonella enterica subsp. enterica serovar java strain nctc5706. this strain is of historical significance, having been isolated in the pre-antibiotic era and was deposited into the national collection of type cultures in 1939.201627811100
complete, closed genome sequences of 10 salmonella enterica subsp. enterica serovar typhimurium strains isolated from human and bovine sources.salmonella enterica is a leading cause of enterocolitis for humans and animals. s. enterica subsp. enterica serovar typhimurium infects a broad range of hosts. to facilitate genomic comparisons among isolates from different sources, we present the complete genome sequences of 10 s typhimurium strains, 5 each isolated from human and bovine sources.201627811097
phenotyping and genetic characterization of salmonella enterica isolates from turkey revealing arise of different features specific to geography.192 food samples (commonly consumed 8 food types), 355 animal samples (animal feces of bovine, ovine, goat and chicken) and 50 samples from clinical human cases in sanliurfa city, turkey in a year were collected to determine the salmonella enterica subsp. enterica mosaic in turkey. 161 salmonella isolates represented 17 serotypes, 20 sequence types (sts) and 44 pfge patterns (pts). 3 serotypes, s. enteritidis, s. typhimurium and s. kentucky, were recovered from three different hosts. the highest ...201727768932
ultrasound improves chemical reduction of natural contaminant microbiota and salmonella enterica subsp. enterica on strawberries.new sanitization methods have been evaluated to improve food safety and food quality and to replace chlorine compounds. however, these new methods can lead to physicochemical and sensory changes in fruits and vegetables. the present study evaluated the effects of acetic acid, peracetic acid, and sodium dodecylbenzenesulfonate isolated or combined with 5min of ultrasound treatment (40khz, 500w) on strawberry quality over 9days of storage at 8°c. the strawberry natural contaminant microbiota (mold ...201727743521
genomic and evolutionary analysis of two salmonella enterica serovar kentucky sequence types isolated from bovine and poultry sources in north america.salmonella enterica subsp. enterica serovar kentucky is frequently isolated from healthy poultry and dairy cows and is occasionally isolated from people with clinical disease. a genomic analysis of 119 isolates collected in the united states from dairy cows, ground beef, poultry and poultry products, and human clinical cases was conducted. results of the analysis demonstrated that the majority of poultry and bovine-associated s. kentucky were sequence type (st) 152. several bovine-associated (n ...201627695032
draft genome sequence of salmonella enterica subsp. enterica serovar orion strain crjjgf_00093 (phylum gammaproteobacteria).here, we report a 4.70-mbp draft genome sequence of salmonella enterica subsp. enterica serovar orion strain crjjgf_00093, isolated from a dog in 2005.201627688320
whole-genome sequencing identifies in vivo acquisition of a blactx-m-27-carrying incfii transmissible plasmid as the cause of ceftriaxone treatment failure for an invasive salmonella enterica serovar typhimurium infection.we report a case of ceftriaxone treatment failure for bacteremia caused by salmonella enterica subsp. enterica serovar typhimurium, due to the in vivo acquisition of a blactx-m-27-encoding incfii group transmissible plasmid. the original β-lactamase-susceptible isolate st882s was replaced by the resistant isolate st931r during ceftriaxone treatment. after relapse, treatment was changed to ciprofloxacin, and the patient recovered. isolate st931r could transfer resistance to escherichia coli at 37 ...201627671066
complete genome sequences of 17 canadian isolates of salmonella enterica subsp. enterica serovar heidelberg from human, animal, and food sources.salmonella enterica subsp. enterica serovar heidelberg is a highly clonal serovar frequently associated with foodborne illness. to facilitate subtyping efforts, we report fully assembled genome sequences of 17 canadian s heidelberg isolates including six pairs of epidemiologically related strains. the plasmid sequences of eight isolates contain several drug resistance genes.201627635008
draft genome sequence of salmonella enterica subsp. enterica serovar bardo strain crjjgf_00099 (phylum gammaproteobacteria).here, we report a 4.87-mbp draft genome sequence of the multidrug-resistant (mdr) salmonella enterica subsp. enterica serovar bardo strain crjjgf_00099, isolated from dairy cattle in 2005.201627634995
draft genome sequence of salmonella enterica subsp. enterica serovar blockley strain crjjgf_00147 (phylum gammaproteobacteria).here, we report a 4.72-mbp draft genome sequence of salmonella enterica subsp. enterica serovar blockley strain crjjgf_00147, isolated from chicken rinse in 2009.201627609923
biofilm formation, phenotypic production of cellulose and gene expression in salmonella enterica decrease under anaerobic conditions.salmonella enterica subsp. enterica is one of the main food-borne pathogens. this microorganism combines an aerobic life outside the host with an anaerobic life within the host. one of the main concerns related to s. enterica is biofilm formation and cellulose production. in this study, biofilm formation, morphotype, cellulose production and transcription of biofilm and quorum sensing-related genes of 11 s. enterica strains were tested under three different conditions: aerobiosis, microaerobiosi ...201627592071
draft genome sequence of salmonella enterica subsp. enterica serovar putten strain crjjgf_00159 (phylum gammaproteobacteria).here, we report a 4.90 mbp draft genome sequence of salmonella enterica subsp. enterica serovar putten strain crjjgf_00159 isolated from food animal in 2004.201627587814
prevalence and characteristics of salmonella and campylobacter in retail poultry meat in japan.this study was undertaken to determine the prevalence, antimicrobial susceptibility, and genetic relatedness of salmonella enterica subsp. enterica and campylobacter spp. in poultry meat, and to analyze the association of genetic types of these bacteria with their geographical distribution and antimicrobial resistance profiles. fifty-four and 71 of 100 samples were found to be contaminated with salmonella and campylobacter, respectively. nine salmonella serotypes were found, including s. enteric ...201627580577
whole-genome sequencing analysis of salmonella enterica serovar enteritidis isolates in chile provides insights into possible transmission between gulls, poultry, and humans.salmonella enterica subsp. enterica serotype enteritidis is a major cause of human salmonellosis worldwide; however, little is known about the genetic relationships between s enteritidis clinical strains and s enteritidis strains from other sources in chile. we compared the whole genomes of 30 s enteritidis strains isolated from gulls, domestic chicken eggs, and humans in chile, to investigate their phylogenetic relationships and to establish their relatedness to international strains. core geno ...201627520817
antimicrobial resistance in zoonotic nontyphoidal salmonella: an alarming trend?zoonotic bacteria of the genus salmonella have acquired various antimicrobial resistance properties over the years. the corresponding resistance genes are commonly located on plasmids, transposons, gene cassettes, or variants of the salmonella genomic islands sgi1 and sgi2. human infections by nontyphoidal salmonella isolates mainly result from ingestion of contaminated food. the two predominantly found salmonella enterica subsp. enterica serovars in the usa and in europe are s. enteritidis and ...201627506509
recovery estimation of dried foodborne pathogens is directly related to rehydration kinetics.drying is a common process which is used to preserve food products and technological microorganisms, but which is deleterious for the cells. the aim of this study is to differentiate the effects of drying alone from the effects of the successive and necessary rehydration. rehydration of dried bacteria is a critical step already studied in starter culture but not for different kinetics and not for pathogens. in the present study, the influence of rehydration kinetics was investigated for three fo ...201627494169
characterization and evaluation of a salmonella enterica serotype senftenberg mutant created by deletion of virulence-related genes for use as a live attenuated vaccine.natural infections of chickens with salmonella enterica subsp. enterica serovar senftenberg (s. senftenberg) are characterized by low-level intestinal invasiveness and insignificant production of antibodies. in this study, we investigated the potential effects of lon and cpxr gene deletions on the invasiveness of s senftenberg into the intestinal epithelium of chickens and its ability to induce an immune response, conferring protection against s senftenberg infection. with the allelic exchange m ...201627489135
draft genome sequences of salmonella enterica subsp. enterica serovars typhimurium and nottingham isolated from food products.a quantitative real-time pcr (qpcr) designed to detect salmonella enterica subsp. enterica serovar enteritidis, targeting the sdf gene, generated positive results for s. enterica subsp. enterica serovar typhimurium (cfsan033950) and s. enterica subsp. enterica serovar nottingham (cfsan006803) isolated from food samples. both strains show pulsed-field gel electrophoresis (pfge) patterns distinct from those of s enteritidis. here, we report the genome sequences of these two strains.201627445384
complete genome sequence of salmonella enterica subsp. enterica serovar indiana c629, a carbapenem-resistant bacterium isolated from chicken carcass in china.the carbapenem-resistant salmonella enterica subsp. enterica serovar indiana strain c629 was isolated from a chicken carcass collected from a slaughterhouse in qingdao, china. the complete genome sequence of c629 contains a circular 4,791,723-bp chromosome and a circular 210,106-bp plasmid. genes involved in carbapenem resistance of this bacterium were identified by whole-genome analysis.201627417837
draft genome sequence of salmonella enterica subsp. enterica serovar widemarsh strain crjjgf_00058 (phylum gammaproteobacteria).here, we report a 4.73 mbp draft genome sequence of salmonella enterica subsp. enterica serovar widemarsh strain crjjgf_00058, isolated from eggs in 2008.201627417830
draft genome sequence of salmonella enterica subsp. enterica serovar lille strain crjjgf_000101 (phylum gammaproteobacteria).here, we report a 4.98 mbp draft genome sequence of salmonella enterica subsp. enterica serovar lille strain crjjgf_000101, isolated from ground beef in 2007.201627417829
detection of a novel qnrb19-carrying plasmid variant mediating decreased fluoroquinolone susceptibility in salmonella enterica serovar hadar.thirty salmonella enterica subsp. enterica serovar hadar isolates of avian origin collected between 2007 and 2010 from chicken carcasses in five geographically spread abattoirs in germany were investigated for plasmid-mediated quinolone resistance determinants. four isolates were identified by pcr analysis and hybridization experiments to carry qnrb genes. the isolates were indistinguishable by their xbai macrorestriction patterns and did not exhibit a mutation in the quinolone resistance-determ ...201727404660
draft genome sequence of salmonella enterica subsp. enterica serovar kiambu strain crjjgf_00061 (phylum gammaproteobacteria).we report a 4.58 mbp draft genome sequence of salmonella enterica subsp. enterica serovar kiambu strain crjjgf_00061 isolated from cattle in 2004.201627389263
experimental infection of chickens by a flagellated motile strain of salmonella enterica serovar gallinarum biovar gallinarum.salmonella enterica subsp. enterica serovar gallinarum biovar gallinarum (sg) causes fowl typhoid (ft), a septicaemic disease which can result in high mortality in poultry flocks. the absence of flagella in sg is thought to favour systemic invasion, since bacterial recognition via toll-like receptor (tlr)-5 does not take place during the early stages of ft. in the present study, chicks susceptible to ft were inoculated with a wild type sg (sg) or its flagellated motile derivative (sg fla(+)). in ...201627387725
identification of bapa in strains of salmonella enterica subsp. enterica isolated from wild animals kept in captivity in sinaloa, mexico.bapa, previously named stm2689, encodes the bapa protein, which, along with cellulose and fimbriae, constitutes biofilms. biofilms are communities of microorganisms that grow in a matrix of exopolysaccharides and may adhere to living tissues or inert surfaces. biofilm formation is associated with the ability to persist in different environments, which contributes to the pathogenicity of several species. we analyzed the presence of bapa in 83 strains belonging to 17 serovars of salmonella enteric ...201627379195
higher storage temperature causes greater salmonella enterica serovar typhimurium internal penetration of artificially contaminated, commercially available, washed free range eggs.foodborne salmonellosis is a major public health concern, with contaminated eggs identified as a significant source of infection. in australia, the most prevalent cause of salmonellosis from eggs is salmonella enterica subsp. enterica serovar typhimurium. this study explored the effect of temperature after 1, 7, 14, 21, and 28 days of storage on commercially available washed free range eggs, artificially contaminated with salmonella typhimurium on the external surface. at each time point, the ex ...201627357046
comparison of microbial communities isolated from feces of asymptomatic salmonella-shedding and non-salmonella shedding dairy cows.in the united states salmonella enterica subsp. enterica serotypes kentucky and cerro are frequently isolated from asymptomatic dairy cows. however, factors that contribute to colonization of the bovine gut by these two serotypes have not been identified. to investigate associations between salmonella status and bacterial diversity, as well as the diversity of the microbial community in the dairy cow hindgut, the bacterial and archaeal communities of fecal samples from cows on a single dairy far ...201627313565
aneurysm of the cranial mesenteric artery as a site of carriage of salmonella enterica subsp. enterica serovar abortusequi in the horse.salmonella enterica subsp. enterica serovar abortusequi is a pathogen restricted to horses. our investigation targeted 4 draft horses (9-10 months old) kept on a japanese farm that had suffered an outbreak of s. abortusequi abortion. the 4 horses were suspected to be carriers of the bacterium owing to their high agglutination titers (≥1:2,560) in tube agglutination testing. the owners' on-farm observations confirmed that the horses had no apparent abnormalities, and s. abortusequi was not isolat ...201627271985
complete and closed genome sequences of 10 salmonella enterica subsp. enterica serovar anatum isolates from human and bovine sources.salmonella enterica is an important pathogen transmitted by numerous vectors. genomic comparisons of salmonella strains from disparate hosts have the potential to further our understanding of mechanisms underlying host specificities and virulence. here, we present the closed genome and plasmid sequences of 10 salmonella enterica subsp. enterica serovar anatum isolates from bovine and human sources.201627257192
molecular identification of salmonella enterica subsp. enterica serovar gallinarum biovars gallinarum and pullorum by a duplex pcr assay.salmonella enterica subsp. enterica serovar gallinarum biovar gallinarum (s gallinarum) and biovar pullorum (s pullorum) are 2 poultry pathogens that cause major economic losses to the poultry industry worldwide. control of both diseases mainly relies on the adoption of biosecurity programs, and success is dependent on accurate and fast detection. based on this concept, we developed a duplex pcr assay, targeting 2 chromosomal sequences, which allowed us to precisely identify and differentiate s ...201627216724
probiotic properties of lactic acid bacteria isolated from human milk.the objective of this study was to identify and characterize lactic acid bacteria isolated from human milk, with an emphasis on their probiotic properties.201627159339
genome sequences of salmonella enterica subsp. enterica serovar lubbock strains isolated from liver abscesses of feedlot cattle.the genome sequencing of 13 salmonella enterica subsp. enterica serovar lubbock strains isolated from liver abscesses of feedlot cattle is reported here. the availability of these genomes will help to further understand the etiologic role of salmonella strains in liver abscesses of cattle and will serve as references in microbial trace-back studies to improve food safety.201627151794
antimicrobial resistance and class 1 integron-associated gene cassettes in salmonella enterica serovar typhimurium isolated from pigs at slaughter and abattoir environment.forty-five multi-resistant salmonella enterica subsp. enterica serovar (s.) typhimurium isolates obtained at five pig abattoirs in southern brazil were characterized. their relatedness was determined by xbai-macrorestriction analysis. resistance genes, integrons and plasmid-mediated quinolone resistance genes (pmqr) were investigated by pcr. amplicons for the variable part of class 1 integrons and the quinolone resistance-determining regions (qrdr) were sequenced. plasmids were characterized by ...201627142182
draft genome sequences of salmonella enterica subsp. enterica serovar berta atcc 8392 and a nalidixic acid-resistant isolate of this strain.italic! salmonella entericasubspecies italic! entericaserovar berta has been isolated in multiple animal species and has been implicated in human disease. here, we report a 4.7-mbp draft genome sequence of italic! s. entericaserovar berta (atcc strain 8392) and a nalidixic acid-resistant isolate derived from this strain.201627103707
salmonella infection and carriage in reptiles in a zoological collection.objective to identify important subspecies and serovars of salmonella enterica in a captive reptile population and clinically relevant risk factors for and signs of illness in salmonella-positive reptiles. design retrospective cross-sectional study. animals 11 crocodilians (4 samples), 78 snakes (91 samples), 59 lizards (57 samples), and 34 chelonians (23 samples) at the bronx zoo from 2000 through 2012. procedures data pertaining to various types of biological samples obtained from reptiles wit ...201627074614
application of antimicrobial agents via commercial spray cabinet to inactivate salmonella on skinless chicken meat.salmonella enterica subsp. enterica serovar typhimurium is a food safety concern for raw poultry products. new and innovative application methods of antimicrobials for the reduction of salmonella in poultry and poultry products are essential. the aim of this study was to determine the efficacy of three antimicrobial compounds against salmonella on raw chicken meat when applied individually and in combination using a commercial spray cabinet. raw chicken thigh meat inoculated with 5 log cfu/g sal ...201627052860
draft whole-genome sequences of salmonella enterica subsp. enterica serovars enteritidis, veneziana, and salford, isolated from herbs.salmonellais a foodborne pathogen found in a wide variety of sources. here, we report draft genome sequences of threesalmonella entericasubsp.entericaserovars found in herbs: enteritidis, veneziana, and salford, with the latter two being extremely rare in california.201627013038
whole genome sequencing provides insights into the genetic determinants of invasiveness in salmonella dublin.salmonella enterica subsp. enterica serovar dublin (s. dublin) is one of the non-typhoidal salmonella (nts); however, a relatively high proportion of human infections are associated with invasive disease. we applied whole genome sequencing to representative invasive and non-invasive clinical isolates of s. dublin to determine the genomic variations among them and to investigate the underlying genetic determinants associated with invasiveness in s. dublin. although no particular genomic variation ...201626996313
broilers with low serum mannose-binding lectin show increased fecal shedding of salmonella enterica serovar montevideo.mannose-binding lectin (mbl) is a key molecule in innate immunity. mbl binds to carbohydrates on the surface of pathogens, initiating the complement system via the lectin-dependent pathway or facilitates opsonophagocytosis. in vivo studies using inbred chicken lines differing in mbl serum concentration indicate that chicken mbl affects salmonella resistance; further studies are imperative in conventional broiler chickens. in this study 104 conventional day-old chickens (offspring from a cross be ...201626994208
bifidobacterium thermophilum rbl67 impacts on growth and virulence gene expression of salmonella enterica subsp. enterica serovar typhimurium.bifidobacterium thermophilum rbl67 (rbl67), a human fecal isolate and health promoting candidate shows antagonistic and protective effects against salmonella and listeria spec. in vitro. however, the underlying mechanisms fostering these effects remain unknown. in this study, the interactions of rbl67 and salmonella enterica subsp. enterica serovar typhimurium n-15 (n-15) were explored by global transcriptional analysis.201626988691
complete genome and methylome sequences of salmonella enterica subsp. enterica serovar panama (atcc 7378) and salmonella enterica subsp. enterica serovar sloterdijk (atcc 15791).salmonella enterica spp. are pathogenic bacteria commonly associated with food-borne outbreaks in human and animals. salmonella enterica spp. are characterized into more than 2,500 different serotypes, which makes epidemiological surveillance and outbreak control more difficult. in this report, we announce the first complete genome and methylome sequences from two salmonella type strains associated with food-borne outbreaks, salmonella enterica subsp. enterica serovar panama (atcc 7378) and salm ...201626988049
draft genome sequence of a salmonella enterica subsp. enterica serovar gallinarum bv. gallinarum isolate associated with fowl typhoid outbreaks in brazil.salmonella enterica subsp. enterica serovar gallinarum bv. gallinarum strains are bird pathogens causing fowl typhoid (ft). isolate br_rs12 was obtained from a poultry flock with ft in 2014. the sequencing of this genome will enable to track the origin of the recent outbreaks in brazil.201626950322
unraveling the effects of static magnetic field stress on cytosolic proteins of salmonella by using a proteomic approach.the present study investigated the adaptation of salmonella enterica subsp. enterica serovar hadar to static magnetic field (smf) exposure (200 mt, 9 h). the proteomic analysis provides an overview of potentially important cytosolic proteins that salmonella needs to regulate to survive and adapt to magnetic stress. via 2-dimensional electrophoresis and liquid chromatography tandem mass spectrometry, we compared cytosolic proteomes before and after exposure to magnetic field. a total of 35 protei ...201626928316
complete closed genome sequences of salmonella enterica subsp. enterica serotypes anatum, montevideo, typhimurium, and newport, isolated from beef, cattle, and humans.salmonella enterica spp. are a diverse group of bacteria with a wide range of virulence potential. to facilitate genome comparisons across this virulence spectrum, we present eight complete closed genome sequences of four s. enterica serotypes (anatum, montevideo, typhimurium, and newport), isolated from various cattle samples and from humans.201626847891
complete genome sequence and methylome of salmonella enterica subsp. enterica cerro, a frequent dairy cow serovar.salmonella enterica subsp. enterica serovar cerro is an infrequent pathogen of humans and other mammals but is frequently isolated from the hindgut of asymptomatic cattle in the united states. to further understand the genomic determinants of s. cerro specificity for the bovine hindgut, the genome of isolate cfsan001588 was fully sequenced and deposited in the genbank database.201626823571
the evaluation and application of multilocus variable number tandem repeat analysis (mlva) for the molecular epidemiological study of salmonella enterica subsp. enterica serovar enteritidis infection.salmonella enterica subsp. enterica serovar enteritidis (s. enteritidis) is one of the most prevalent salmonella serotypes that cause gastroenteritis worldwide and the most prevalent serotype causing salmonella infections in china. a rapid molecular typing method with high throughput and good epidemiological discrimination is urgently needed for detecting the outbreaks and finding the source for effective control of s. enteritidis infections.201626823185
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