Publications

characterization of the complete mitogenome of anopheles aquasalis, and phylogenetic divergences among anopheles from diverse geographic zones.	whole mitogenome sequences (mtdna) have been exploited for insect ecology studies, using them as molecular markers to reconstruct phylogenies, or to infer phylogeographic relationships and gene flow. recent anopheles phylogenomic studies have provided information regarding the time of deep lineage divergences within the genus. here we report the complete 15,393 bp mtdna sequences of anopheles aquasalis, a neotropical human malaria vector. when comparing its structure and base composition with ot ...	2019	31479460
species diversity and abundance of anopheles (nyssorhynchus) (diptera: culicidae) in cachoeiras de macacu municipality, rio de janeiro state: an area of the atlantic forest receptive and vulnerable to malaria.	the present study evaluated the diversity of anopheles meigen, 1818 belonging to the subgenus nyssorhynchus blanchard, 1902 in four areas of cachoeiras de macacu municipality, rio de janeiro, brazil. the immature collections were carried out during a rainy period and a post-rainy season. during the two periods, 3,932 specimens of anopheles (nyssorhynchus) were collected: 562 in the rainy period (14.29%) and 3,370 in the post-rainy period (85.70%). among 3,932 specimens collected during both peri ...	2019	30649408
composition of anopheline (diptera: culicidae) community and its seasonal variation in three environments of the city of puerto iguazú, misiones, argentina.	in order to extend the knowledge of the composition of the anopheline community and the seasonal variation related to anthropogenic modifications in the city of puerto iguazú, adult females were captured between 2009 and 2012. samples were collected in three environments with different degrees of anthropogenic modification: urban, periurban, and wild. alpha diversity was evaluated as the 'true' diversity of the species in each environment. among environments, range-abundance curves were used to ...	2017	29194513
an evaluation of the suitability of coi and coii gene variation for reconstructing the phylogeny of, and identifying cryptic species in, anopheline mosquitoes (diptera culicidae).	we assessed the practicality and effectiveness of using variation in the mitochondrial coi and coii genes to discriminate species and reconstruct the phylogeny of anophelene mosquitoes. phylogenetic relationships among the subfamily anophelinae were inferred from portions of the mitochondrial coi (92 species) and coii genes (108 species). phylogenetic trees were reconstructed on the basis of parsimony, maximum likelihood and bayesian methods. the suitability of coi and coii gene variation for id ...	2016	27937065
the phylogenetic relationships of known mosquito (diptera: culicidae) mitogenomes.	the known mosquito mitogenomes, containing a total of 34 species, which belong to five genera, were collected from genbank, and the practicality and effectiveness of the variation in the complete mitochondrial dna genome and portions of mitochondrial coi gene were assessed to reconstruct the phylogeny of mosquitoes. phylogenetic trees were reconstructed on the basis of parsimony, maximum likelihood, and bayesian (bi) methods. it is concluded that: (1) both mitogenomes and coi gene support the mo ...	2016	27731651
filling gaps on ivermectin knowledge: effects on the survival and reproduction of anopheles aquasalis, a latin american malaria vector.	strategies designed to advance towards malaria elimination rely on the detection and treatment of infections, rather than fever, and the interruption of malaria transmission between mosquitoes and humans. mass drug administration with anti-malarials directed at eliminating parasites in blood, either to entire populations or targeting only those with malaria infections, are considered useful strategies to progress towards malaria elimination, but may be insufficient if applied on their own. these ...	2016	27660149
spatial distributions of anopheles species in relation to malaria incidence at 70 localities in the highly endemic northwest and south pacific coast regions of colombia.	a proper identification of malaria vectors is essential for any attempt to control this disease. between 40 and 47 anopheles species have been recorded in colombia, and eight species complexes have been identified in the last decade. an update of anopheles species distribution and its relationship with malaria is required, particularly for newly identified members of species complexes.	2016	27515166
comparative mt genomics of the tipuloidea (diptera: nematocera: tipulomorpha) and its implications for the phylogeny of the tipulomorpha.	a traditionally controversial taxon, the tipulomorpha has been frequently discussed with respect to both its familial composition and relationships with other nematocera. the interpretation of internal relationships within the tipuloidea, which include the tipulidae sensu stricto, cylindrotomidae, pediciidae and limoniidae, is also problematic. we sequenced the first complete mitochondrial (mt) genome of symplecta hybrida (meigen, 1804), which belongs to the subfamily chioneinae of family limoni ...	2016	27341029
mitochondrial genomes of anopheles (kerteszia) (diptera: culicidae) from the atlantic forest, brazil.	mitochondrial genome sequences are widely used as molecular markers for phylogenetic studies of mosquito species complexes, such as the anopheles albitarsis complex. except for a few studies that employed a limited number of nuclear or mitochondrial loci to address the genetic structure and species status of anopheles cruzii, anopheles bellator, and anopheles homunculus, little is known about genetic markers that can be employed in studies focusing on kerteszia species. the complete mitochondria ...	2016	27146682
comparative studies on the stenogamous and eurygamous behavior of eight anopheles species of the hyrcanus group (diptera: culicidae) in thailand.	establishment of laboratory colony is essential for mosquito-borne-disease research. mating behavior of stenogamous anopheles peditaeniatus and seven eurygamous species (anopheles argyropus, anopheles crawfordi, anopheles nigerrimus, anopheles nitidus, anopheles paraliae (=an. lesteri), anopheles pursati and anopheles sinensis), were investigated and compared in this study. the self-mating success of adult mosquitoes in different size cages at two density resting surface (drs) values, 3.6 and 7. ...	2016	27023618
frequent blood feeding enables insecticide-treated nets to reduce transmission by mosquitoes that bite predominately outdoors.	the effectiveness of vector control on malaria transmission by long-lasting insecticidal nets (llins) and indoor residual spraying (irs) depends on the vectors entering houses to blood feed and rest when people are inside houses. in the solomon islands, significant reductions in malaria have been achieved in the past 20 years with insecticide-treated bed nets, irs, improved diagnosis and treatment with artemisinin combination therapies; despite the preference of the primary vector, anopheles far ...	2016	26969430
epidemiological and entomological studies of a malaria outbreak among french armed forces deployed at illegal gold mining sites reveal new aspects of the disease's transmission in french guiana.	in december 2010, a plasmodium vivax malaria outbreak occurred among french forces involved in a mission to control illegal gold mining in french guiana. the findings of epidemiological and entomological investigations conducted after this outbreak are presented here.	2016	26801629
contribution of fish farming ponds to the production of immature anopheles spp. in a malaria-endemic amazonian town.	in the past decade fish farming has become an important economic activity in the occidental brazilian amazon, where the number of new fish farms is rapidly increasing. one of the primary concerns with this phenomenon is the contribution of fishponds to the maintenance and increase of the anopheline mosquito population, and the subsequent increase in human malaria burden. this study reports the results of a 2-year anopheline abundance survey in fishponds and natural water bodies in a malaria-ende ...	2015	26573145
new classification of natural breeding habitats for neotropical anophelines in the yanomami indian reserve, amazon region, brazil and a new larval sampling methodology.	here we present the first in a series of articles about the ecology of immature stages of anophelines in the brazilian yanomami area. we propose a new larval habitat classification and a new larval sampling methodology. we also report some preliminary results illustrating the applicability of the methodology based on data collected in the brazilian amazon rainforest in a longitudinal study of two remote yanomami communities, parafuri and toototobi. in these areas, we mapped and classified 112 na ...	0	26517655
a historical perspective on malaria control in brazil.	malaria has always been an important public health problem in brazil. the early history of brazilian malaria and its control was powered by colonisation by europeans and the forced relocation of africans as slaves. internal migration brought malaria to many regions in brazil where, given suitable anopheles mosquito vectors, it thrived. almost from the start, officials recognised the problem malaria presented to economic development, but early control efforts were hampered by still developing pub ...	0	26517649
mitochondrial genomes and comparative analyses of culex camposi, culex coronator, culex usquatus and culex usquatissimus (diptera:culicidae), members of the coronator group.	the coronator group currently encompasses six morphologically similar species (culex camposi dyar, culex coronator dyar and knab, culex covagarciai forattini, culex usquatus dyar, culex usquatissimus dyar, and culex ousqua dyar). culex coronator has been incriminated as a potential vector of west nile virus (wnv), saint louis encephalitis virus (slev), and venezuelan equine encephalitis virus (veev). the complete mitochondrial genome of cx. coronator, cx. usquatus, cx.usquatissimus, and cx. camp ...	2015	26489754
evidence for temporal population replacement and the signature of ecological adaptation in a major neotropical malaria vector in amazonian peru.	the major neotropical malaria vector, anopheles darlingi, was reintroduced into the iquitos, loreto, peru area during the early 1990s, where it displaced other anophelines and caused a major malaria epidemic. since then, case numbers in loreto have fluctuated, but annual increases have been reported since 2012.	2015	26415942
phenotypic and genotypic variations among three allopatric populations of lutzomyia umbratilis, main vector of leishmania guyanensis.	in south america, lutzomyia umbratilis is the main vector of leishmania guyanensis, one of the species involved in the transmission of american tegumentary leishmaniasis. in brazil, l. umbratilis has been recorded in the amazon region, and in the state of pernambuco, northeastern region, where an isolated population has been identified. this study assessed the phylogeographic structure and size and shape differences of the wing of three brazilian populations.	2015	26338469
first record of anopheles oryzalimnetes, anopheles argyritarsis, and anopheles sawyeri (diptera: culicidae) in the caatinga biome, semiarid scrubland of sergipe state, brazil.	caatinga is one of the least known biomes of brazil in relation to biodiversity. the dry condition of semiarid areas has been associated in the past with low richness of fauna and flora, not encouraging studies in this region. there is a lack of mosquito records including anophelines. thus, to investigate the biodiversity of anopheles mosquitoes in the caatinga biome, we collected immature mosquitoes in aquatic habitats in a conservation reserve located in the northwestern portion of sergipe sta ...	2015	26336257
malaria vectors in south america: current and future scenarios.	malaria remains a significant public health issue in south america. future climate change may influence the distribution of the disease, which is dependent on the distribution of those anopheles mosquitoes competent to transmit plasmodium falciparum. herein, predictive niche models of the habitat suitability for p. falciparum, the current primary vector anopheles darlingi and nine other known and/or potential vector species of the neotropical albitarsis complex, were used to document the current ...	2015	26283539
diversification of the genus anopheles and a neotropical clade from the late cretaceous.	the anopheles genus is a member of the culicidae family and consists of approximately 460 recognized species. the genus is composed of 7 subgenera with diverse geographical distributions. despite its huge medical importance, a consensus has not been reached on the phylogenetic relationships among anopheles subgenera. we assembled a comprehensive dataset comprising the coi, coii and 5.8s rrna genes and used maximum likelihood and bayesian inference to estimate the phylogeny and divergence times o ...	2015	26244561
purification methodology for viable and infective plasmodium vivax gametocytes that is compatible with transmission-blocking assays.	significant progress toward the control of malaria has been achieved, especially regarding plasmodium falciparum infections. however, the unique biology of plasmodium vivax hampers current control strategies. the early appearance of p. vivax gametocytes in the peripheral blood and the impossibility of culturing this parasite are major drawbacks. using blood samples from 40 p. vivax-infected patients, we describe here a methodology to purify viable gametocytes and further infect anophelines. this ...	2015	26239989
seasonal distribution of malaria vectors (diptera: culicidae) in rural localities of porto velho, rondônia, brazilian amazon.	we conducted a survey of the malaria vectors in an area where a power line had been constructed, between the municipalities of porto velho and rio branco, in the states of rondônia and acre, respectively. the present paper relates to the results of the survey of anopheles fauna conducted in the state of rondônia. mosquito field collections were performed in six villages along the federal highway br 364 in the municipality of porto velho, namely porto velho, jaci paraná, mutum paraná, vila abunã, ...	0	26200969
establishment of a free-mating, long-standing and highly productive laboratory colony of anopheles darlingi from the peruvian amazon.	anopheles darlingi is the main malaria vector in the amazon region and is among the most efficient malaria vectors worldwide. however, due to the lack of a well-established laboratory colony, key control-relevant aspects of the bionomics, behaviour, genetics, and vector-parasite relationships of an. darlingi remain unknown. here, biological parameters that had been successful in initiating other anopheles colonies were optimized and improved for an. darlingi, with the aim of establish a free-mat ...	2015	26024853
coffee and its waste repel gravid aedes albopictus females and inhibit the development of their embryos.	dengue is a prevalent arboviral disease and the development of insecticide resistance among its vectors impedes endeavors to control it. coffee is drunk by millions of people daily worldwide, which is associated with the discarding of large amounts of waste. coffee and its waste contain large amounts of chemicals many of which are highly toxic and none of which have a history of resistance in mosquitoes. once in solution, coffee is brownish in colour, resembling leaf infusion, which is highly at ...	2015	25966847
wing sexual dimorphism of pathogen-vector culicids.	sexual dimorphism in animals has been studied from different perspectives for decades. in 1874 darwin hypothesized that it was related to sexual selection, and even after nearly 140 years, when additional empirical data has become available and the subject has been investigated from a contemporary viewpoint, this idea is still supported. although mosquito (culicidae) wings are of great importance as they play a sex-specific role, little is known about wing sexual dimorphism in these pathogen-vec ...	2015	25890192
the influence of the area of the serra da mesa hydroelectric plant, state of goiás, on the frequency and diversity of anophelines (diptera: culicidae): a study on the effect of a reservoir.	bioecological aspects of anophelines (diptera: culicidae) near areas under the direct influence of the hydroelectric plant reservoir of serra da mesa in goiás, brazil, were analyzed.	2015	25860461
a nuclear ribosomal dna pseudogene in triatomines opens a new research field of fundamental and applied implications in chagas disease.	a pseudogene, designated as "ps(5.8s+its-2)", paralogous to the 5.8s gene and internal transcribed spacer (its)-2 of the nuclear ribosomal dna (rdna), has been recently found in many triatomine species distributed throughout north america, central america and northern south america. among characteristics used as criteria for pseudogene verification, secondary structures and free energy are highlighted, showing a lower fit between minimum free energy, partition function and centroid structures, a ...	2015	25760450
an overview of malaria transmission from the perspective of amazon anopheles vectors.	in the americas, areas with a high risk of malaria transmission are mainly located in the amazon forest, which extends across nine countries. one keystone step to understanding the plasmodium life cycle in anopheles species from the amazon region is to obtain experimentally infected mosquito vectors. several attempts to colonise anopheles species have been conducted, but with only short-lived success or no success at all. in this review, we review the literature on malaria transmission from the ...	2015	25742262
hybrid sterility in crosses between two brazilian sibling species of the anopheles albitarsis complex.	complexes of cryptic species are common in several taxa and this is also the case in the anopheles genus, a group including all known human malaria vectors. the anopheles albitarsis complex comprises at least nine cryptic species, some of which are implicated as vectors of human malaria. several different types of data have been generated for this species complex such as cytogenetics, alloenzymes, morphological and feeding behavioral, hybridization experiments, rapd-pcr and rflp and mitochondria ...	2014	25471342
anopheles species composition explains differences in plasmodium transmission in la guajira, northern colombia.	malaria in la guajira, the most northern state of colombia, shows two different epidemiological patterns. malaria is endemic in the municipality of dibulla whereas in riohacha it is characterised by sporadic outbreaks. this study aimed to establish whether differences in transmission patterns could be attributed to different vector species. the most abundant adult female species were anopheles aquasalis, exclusive to riohacha, and anopheles darlingi, restricted to dibulla. anopheles mosquitoes w ...	0	25411002
mutations in the voltage-gated sodium channel gene of anophelines and their association with resistance to pyrethroids - a review.	constant and extensive use of chemical insecticides has created a selection pressure and favored resistance development in many insect species worldwide. one of the most important pyrethroid resistance mechanisms is classified as target site insensitivity, due to conformational changes in the target site that impair a proper binding of the insecticide molecule. the voltage-gated sodium channel (nav) is the target of pyrethroids and ddt insecticides, used to control insects of medical, agricultur ...	2014	25292318
the remarkable journey of adaptation of the plasmodium falciparum malaria parasite to new world anopheline mosquitoes.	plasmodium falciparum originated in africa, dispersed around the world as a result of human migration and had to adapt to several different indigenous anopheline mosquitoes. anophelines from the new world are evolutionary distant form african ones and this probably resulted in a more stringent selection of plasmodium as it adapted to these vectors. it is thought that plasmodium has been genetically selected by some anopheline species through unknown mechanisms. the mosquito immune system can gre ...	0	25185006
malaria vectors in san josé del guaviare, orinoquia, colombia.	this study was conducted to determine anopheles species composition and their natural infectivity by human plasmodium in 2 localities with the highest malaria transmission in san jose del guaviare, guaviare, colombia. a total of 1,009 anopheles mosquitoes were collected using human landing catches during 8 months in 2010. anopheles darlingi was the most abundant (83.2%) followed by an. albitarsis s.l. (8.6%), anopheles braziliensis (3.8%), an. oswaldoi s.l. (1%), and an. rangeli (0.3%). anophele ...	0	25102591
sex and rhythms in sandflies and mosquitoes: an appreciation of the work of alexandre afranio peixoto (1963-2013).	i will briefly discuss the work of alexandre a. peixoto on sandflies and mosquitoes, focusing initially on his contributions to the population biology and phylogenetics of brazilian populations of these important hematophagous insects. i shall also review some of his work on the underlying molecular clocks that mediate rhythmic behaviour and physiology in these species.	2014	25046172
the modulation of the symbiont/host interaction between wolbachia pipientis and aedes fluviatilis embryos by glycogen metabolism.	wolbachia pipientis, a maternally transmitted bacterium that colonizes arthropods, may affect the general aspects of insect physiology, particularly reproduction. wolbachia is a natural endosymbiont of aedes fluviatilis, whose effects in embryogenesis and reproduction have not been addressed so far. in this context, we investigated the correlation between glucose metabolism and morphological alterations during a. fluviatilis embryo development in wolbachia-positive (w+) and wolbachia-negative (w ...	2014	24926801
geographic distribution, evolution, and disease importance of species within the neotropical anopheles albitarsis group (diptera, culicidae).	the anopheles albitarsis group of mosquitoes comprises eight recognized species and one mitochondrial lineage. our knowledge of malaria vectorial importance and the distribution and evolution of these taxa is incomplete. we constructed ecological niche models (enms) for these taxa and used hypothesized phylogenetic relationships and enms to investigate environmental and ecological divergence associated with speciation events. two major clades were identified, one north (clade 1) and one south (c ...	0	24820570
experimental plasmodium vivax infection of key anopheles species from the brazilian amazon.	anopheles darlingi is the major malaria vector in countries located in the amazon region. anopheles aquasalis and anopheles albitarsis s.l. are also proven vectors in this region. anopheles nuneztovari s.l. and anopheles triannulatus s.l. were found infected with plasmodium vivax; however, their status as vectors is not yet well defined. knowledge of susceptibility of amazon anopheline populations to plasmodium infection is necessary to better understand their vector capacity. laboratory coloniz ...	2013	24359307
effects of drying eggs and egg storage on hatchability and development of anopheles arabiensis.	the mass rearing of insects requires a large colony from which individuals can be harvested for sterilization and release. attention is given to larval food requirements and to handling and rearing conditions to ensure predictability and synchrony of development. maximizing production requires optimized adult holding to ensure mating success, blood feeding and oviposition. appropriate egg storage and harvesting is necessary to compensate any unpredicted reduction in egg production.	2013	24028497
on the use of classic epidemiological formulae for estimating the intensity of endemic malaria transmission by vectors in the amazon.	although various reports have described entomological inoculation rates of malaria vector species, most were limited to providing descriptive field data. here, we report biting rates and survival data for two important malaria vectors in the amazon, anopheles darlingi (root) and anopheles albitarsis e (lynch-arribalzaga) (diptera: culicidae), in the state of roraima, brazil. we calculated theoretical sporozoite infection rates and critical vector biting rates for these species during 1 year, com ...	2012	23950095
comparison of transmission parameters between anopheles argyritarsis and anopheles pseudopunctipennis in two ecologically different localities of bolivia.	anopheles (anopheles) pseudopunctipennis is a recognized malaria vector in the slopes of the andes of bolivia. there, other species might be involved in malaria transmission and one candidate could be anopheles argyritarsis. although it is generally admitted that this species is not a malaria vector in the neotropical region, its potential role in transmission is still controversial and this situation has to be cleared, at least for bolivia. comparing the vectorial efficiency of an. pseudopuncti ...	2013	23941216
culicidae (diptera) selection of humans, chickens and rabbits in three different environments in the province of chaco, argentina.	studies were conducted to determine the selection of humans, chickens and rabbits by culicidae in three different environments in the province of chaco, argentina. mosquitoes were collected fortnightly using cylindrical metal traps containing animal bait (chickens and rabbits). the mosquitoes were collected between june 2001-may 2002. during the same period and with the same frequency, mosquitoes biting the human operators of the traps were collected during the first 15 min of exposure within di ...	0	23903970
land cover, land use and malaria in the amazon: a systematic literature review of studies using remotely sensed data.	the nine countries sharing the amazon forest accounted for 89% of all malaria cases reported in the americas in 2008. remote sensing can help identify the environmental determinants of malaria transmission and their temporo-spatial evolution. seventeen studies characterizing land cover or land use features, and relating them to malaria in the amazon subregion, were identified. these were reviewed in order to improve the understanding of the land cover/use class roles in malaria transmission. the ...	2013	23758827
malaria in south america: a drug discovery perspective.	the challenge of controlling and eventually eradicating malaria means that new tools are urgently needed. south america's role in this fight spans both ends of the research and development spectrum: both as a continent capable of discovering and developing new medicines, and also as a continent with significant numbers of malaria patients. this article reviews the contribution of groups in the south american continent to the research and development of new medicines over the last decade. therefo ...	2013	23706107
wing geometric morphometrics and molecular assessment of members in the albitarsis complex from colombia.	malaria parasites are transmitted to humans by female mosquitoes of the genus anopheles. the albitarsis complex harbours at least eight species not readily differentiable by morphology. this complicates the determination of those species involved in malaria transmission and the implementation of targeted and effective vector control strategies. in colombia, there is little information about the identity and distribution of the albitarsis complex members. in this work, coi dna barcoding was used ...	2013	23702155
host range of the parasite strelkovimermis spiculatus (nematoda: mermithidae) in argentina mosquitoes.	strelkovimermis spiculatus (poinar and camino 1986) is a nematode parasite of mosquitoes isolated from the neotropical region. we investigated the host range of this parasite in mosquito populations for a better understanding of the dynamics of temporary breeding sites. five grassy-pool habitats filled by rainwater were sampled from the summer 2007 through the fall 2008. eight mosquito species were collected: anopheles albitarsis, culex chidesteri, culex dolosus, culex maxi, aedes albifasciatus, ...	2013	23701609
the mitochondrial genome of elodia flavipalpis aldrich (diptera: tachinidae) and the evolutionary timescale of tachinid flies.	tachinid flies are natural enemies of many lepidopteran and coleopteran pests of forests, crops, and fruit trees. in order to address the lack of genetic data in this economically important group, we sequenced the complete mitochondrial genome of the palaearctic tachinid fly elodia flavipalpis aldrich, 1933. usually found in northern china and japan, this species is one of the primary natural enemies of the leaf-roller moths (tortricidae), which are major pests of various fruit trees. the 14,932 ...	2013	23626734
malaria entomological risk factors in relation to land cover in the lower caura river basin, venezuela.	to explore the effects of deforestation and resulting differences in vegetation and land cover on entomological parameters, such as anopheline species composition, abundance, biting rate, parity and entomological inoculation rate (eir), three villages were selected in the lower caura river basin, state of bolívar, venezuela. all-night mosquito collections were conducted between march 2008-january 2009 using cdc light traps and mosquito magnet® liberty plus. human landing catches were performed b ...	0	23579803
entomological surveillance of behavioural resilience and resistance in residual malaria vector populations.	the most potent malaria vectors rely heavily upon human blood so they are vulnerable to attack with insecticide-treated nets (itns) and indoor residual spraying (irs) within houses. mosquito taxa that can avoid feeding or resting indoors, or by obtaining blood from animals, mediate a growing proportion of the dwindling transmission that persists as itns and irs are scaled up.	2013	23577656
ecological suitability and spatial distribution of five anopheles species in amazonian brazil.	seventy-six sites characterized in amazonian brazil revealed distinct habitat diversification by examining the environmental factors associated with the distribution and abundance of five anopheline species (diptera: culicidae) in the subgenus nyssorhynchus. these included three members of the albitarsis complex, anopheles oryzalimnetes, anopheles marajoara, anopheles janconnae; anopheles triannulatus, and anopheles goeldii. anopheles janconnae abundance had a positive correlation to water flow ...	2013	23546804
abundance, behavior and entomological inoculation rates of anthropophilic anophelines from a primary colombian malaria endemic area.	in colombia for several years, the urabá-bajo cauca and alto sinú region has registered the highest numbers of malaria cases in the country. malaria vector incrimination and the characterization of entomological parameters will allow for a better understanding of malaria transmission dynamics and the design of effective vector control strategies for this region.	2013	23497535
ecology of anopheline mosquitoes (diptera: culicidae) in the central atlantic forest biodiversity corridor, southeastern brazil.	knowledge of the fauna composition of anopheline mosquitoes, their ecological aspects and behavior, and influence of climatic variables on their population dynamics can help in understanding the transmission of plasmodium parasites and thus develop more efficient strategies for the control of malaria. in the central atlantic forest biodiversity corridor, southeastern brazil, foci of introduced malaria have been reported among people returning from the amazon region, north brazil. our objective w ...	2013	23427648
mitochondrial genome sequences reveal deep divergences among anopheles punctulatus sibling species in papua new guinea.	members of the anopheles punctulatus group (ap group) are the primary vectors of human malaria in papua new guinea. the ap group includes 13 sibling species, most of them morphologically indistinguishable. understanding why only certain species are able to transmit malaria requires a better comprehension of their evolutionary history. in particular, understanding relationships and divergence times among anopheles species may enable assessing how malaria-related traits (e.g. blood feeding behavio ...	2013	23405960
confirmation of the occurrence of anopheles (nyssorhynchus) marajoara in french guiana.	throughout the early entomological campaigns in french guiana (1900-1945), the presence of members of the anopheles albitarsis complex was reported in many places across the territory. however, since then no specimen has been caught despite many entomological studies conducted on the littoral and along the main rivers in places where malaria was endemic. we report here the 1st catches in the modern period of specimens of the an. albitarsis complex in the deep rainforest. during a military interv ...	2012	23393754
phylogenetic analysis and dna-based species confirmation in anopheles (nyssorhynchus).	specimens of neotropical anopheles (nyssorhynchus) were collected and identified morphologically. we amplified three genes for phylogenetic analysis-the single copy nuclear white and cad genes, and the coi barcode region. since we had multiple specimens for most species we were able to test how well the single or combined genes were able to corroborate morphologically defined species by placing the species into exclusive groups. we found that single genes, including the coi barcode region, were ...	2013	23390494
isolation and characterization of 25 microsatellite dna loci for anopheles albitarsis sensu lato and inter-specific amplification in 5 congeneric species.	the anopheles albitasis complex includes 6 species, and 3 are considered as malaria vectors in brazil. twenty-five polymorphic microsatellite dna loci were isolated and characterized in 24-36 individuals from the neighborhood of puraquequara, manaus, amazonas state, brazil. the number of estimated alleles ranged from 2 to 10, the observed heterozygosity ranged from 0.182 to 0.897, and the expected heterozygosity ranged from 0.260 to 0.854. eleven loci showed significant deviation from hardy-wein ...	2012	23315804
[anopheles (díptera: culicidae) vectors of malaria in puerto carreño municipality, vichada, colombia].	the study of the biological aspects of anopheles spp., strengthens the entomological surveillance.	0	23235809
the effects of urbanization on global plasmodium vivax malaria transmission.	many recent studies have examined the impact of urbanization on plasmodium falciparum malaria endemicity and found a general trend of reduced transmission in urban areas. however, none has examined the effect of urbanization on plasmodium vivax malaria, which is the most widely distributed malaria species and can also cause severe clinical syndromes in humans. in this study, a set of 10,003 community-based p. vivax parasite rate (pvpr) surveys are used to explore the relationships between pvpr i ...	2012	23217010
feeding patterns of mosquitoes (diptera: culicidae) in six brazilian environmental preservation areas.	feeding patterns of mosquitoes in six brazilian environmental preservation areas were analyzed by the precipitin technique. the mosquito populations were captured using shannon traps during different time periods. bird, cow, dog, horse, opossum, human, and rodent antisera diagnostic tests were employed and results were analyzed by calculating the sørensen similarity index and using the null-model test. of the 647 analyzed specimens, 443 reacted to the utilized antisera, of which 331 reacted to o ...	2012	23181858
integrated vector management targeting anopheles darlingi populations decreases malaria incidence in an unstable transmission area, in the rural brazilian amazon.	studies on vector behaviour should be conducted in order to evaluate the effectiveness of vector control measures on malaria protection in endemic areas of latin america, where p. vivax predominates. this work aims to investigate the fauna of anopheline mosquitoes and verify the impact of integrated vector management in two colonization projects in the careiro municipality, western brazilian amazon.	2012	23088224
mermithid nematodes found in adult anopheles from southeastern senegal.	over two dozen mermithid nematodes have been described parasitizing mosquitoes worldwide, however, only two species were found in africa. mermithid nematodes kill their mosquito host upon emergence, which suggests that they could be developed as biological control agents of mosquitoes. both romanomermis culicivorax and romanomermis iyengari have been reared for mass release to control numerous anopheles species vector populations, and in one instance this may have led to reduced malaria prevalen ...	2012	22741946
dna barcoding reveals both known and novel taxa in the albitarsis group (anopheles: nyssorhynchus) of neotropical malaria vectors.	mosquitoes belonging to the albitarsis group (anopheles: nyssorhynchus) are of importance as malaria vectors across the neotropics. the group currently comprises six known species, and recent studies have indicated further hidden biodiversity within the group. dna barcoding has been proposed as a highly useful tool for species recognition, although its discriminatory utility has not been verified in closely related taxa across a wide geographic distribution.	2012	22353437
silent circulation of st. louis encephalitis virus prior to an encephalitis outbreak in cordoba, argentina (2005).	st. louis encephalitis virus is a complex zoonoses. in 2005, 47 laboratory-confirmed and probable clinical cases of slev infection were reported in córdoba, argentina. although the causes of 2005 outbreak remain unknown, they might be related not only to virological factors, but also to ecological and environmental conditions. we hypothesized that one of the factors for sle reemergence in córdoba, argentina, was the introduction of a new slev genotype (slev genotype iii), with no previous activi ...	2012	22303490
variations of insecticide residual bio-efficacy on different types of walls: results from a community-based trial in south cameroon.	determination of residual activity of insecticides is essential information for the selection of appropriate indoor spraying operation. the present study was undertaken to evaluate the residual effect of three candidate insecticide formulations on different indoor surfaces in order to guide future interventions, in the context of cameroon and other african countries.	2011	22047173
classification of immature mosquito species according to characteristics of the larval habitat in the subtropical province of chaco, argentina.	to classify mosquito species based on common features of their habitats, samples were obtained fortnightly between june 2001-october 2003 in the subtropical province of chaco, argentina. data on the type of larval habitat, nature of the habitat (artificial or natural), size, depth, location related to sunlight, distance to the neighbouring houses, type of substrate, organic material, vegetation and algae type and their presence were collected. data on the permanence, temperature, ph, turbidity, ...	2011	21739026
population dynamics, structure and behavior of anopheles darlingi in a rural settlement in the amazon rainforest of acre, brazil.	anopheles darlingi is the major vector of malaria in south america, and its behavior and distribution has epidemiological importance to biomedical research. in brazil, an. darlingi is found in the northern area of the amazon basin, where 99.5% of the disease is reported.	2011	21702964
laboratory selection for an accelerated mosquito sexual development rate.	abstract: background: separating males and females at the early adult stage did not ensure the virginity of females of anopheles arabiensis (dongola laboratory strain), whereas two years earlier this method had been successful. in most mosquito species, newly emerged males and females are not able to mate successfully. for anopheline species, a period of 24 h post-emergence is generally required for the completion of sexual maturation, which in males includes a 180degrees rotation of the genital ...	2011	21595988
evaluation of a pcr-rflp-its2 assay for discrimination of anopheles species in northern and western colombia.	anopheles mosquitoes are routinely identified using morphological characters of the female that often lead to misidentification due to interspecies similarity and intraspecies variability. the aim of this work was to evaluate the applicability of a previously developed pcr-rflp-its2 assay for accurate discrimination of anophelines in twelve localities spanning three colombian malaria epidemiological regions: atlantic coast, pacific coast, and uraba-bajo cauca-alto sinu region. the evaluation of ...	2011	21345325
identification of a fibrinogen-related protein (fbn9) gene in neotropical anopheline mosquitoes.	malaria has a devastating impact on worldwide public health in many tropical areas. studies on vector immunity are important for the overall understanding of the parasite-vector interaction and for the design of novel strategies to control malaria. a member of the fibrinogen-related protein family, fbn9, has been well studied in anopheles gambiae and has been shown to be an important component of the mosquito immune system. however, little is known about this gene in neotropical anopheline speci ...	2011	21288344
analysis of the evolutionary forces shaping mitochondrial genomes of a neotropical malaria vector complex.	many vectors of human malaria belong to complexes of morphologically indistinguishable cryptic species. here we report the analysis of the newly sequenced complete mitochondrial dna molecules from six recognized or putative species of one such group, the neotropical anopheles albitarsis complex. the molecular evolution of these genomes had been driven by purifying selection, particularly strongly acting on the rna genes. directional mutation pressure associated with the strand-asynchronous asymm ...	2011	21241811
a new mtdna coi gene lineage closely related to anopheles janconnae of the albitarsis complex in the caribbean region of colombia.	an understanding of the taxonomic status and vector distribution of anophelines is crucial in controlling malaria. previous phylogenetic analyses have supported the description of six species of the neotropical malaria vector anopheles (nyssorhynchus) albitarsis s.l. (diptera: culicidae): an. albitarsis, anopheles deaneorum, anopheles marajoara, anopheles oryzalimnetes, anopheles janconnae and an. albitarsis f. to evaluate the taxonomic status of an. albitarsis s.l. mosquitoes collected in vario ...	0	21225199
heritability and adaptive phenotypic plasticity of adult body size in the mosquito aedes aegypti with implications for dengue vector competence.	adaptive phenotypic plasticity is particularly important to organisms with developmental cycles that undergo ontogenetic niche shifts that differentially subject individual life stages to heterogeneous and often stressful environmental conditions. the yellow fever and dengue fever vector mosquito, aedes aegypti, typically breeds in small water-filled containers that expose the developing aquatic larvae to competition for resources with conspecifics and high probabilities for habitat drying. here ...	2010	21070891
lineage divergence detected in the malaria vector anopheles marajoara (diptera: culicidae) in amazonian brazil.	cryptic species complexes are common among anophelines. previous phylogenetic analysis based on the complete mtdna coi gene sequences detected paraphyly in the neotropical malaria vector anopheles marajoara. the "folmer region" detects a single taxon using a 3% divergence threshold.	2010	20929572
the dominant anopheles vectors of human malaria in the americas: occurrence data, distribution maps and bionomic précis.	abstract:	2010	20712879
anopheles aquasalis infected by plasmodium vivax displays unique gene expression profiles when compared to other malaria vectors and plasmodia.	malaria affects 300 million people worldwide every year and is endemic in 22 countries in the americas where transmission occurs mainly in the amazon region. most malaria cases in the americas are caused by plasmodium vivax, a parasite that is almost impossible to cultivate in vitro, and anopheles aquasalis is an important malaria vector. understanding the interactions between this vector and its parasite will provide important information for development of disease control strategies. to this e ...	2010	20339545
germ band retraction as a landmark in glucose metabolism during aedes aegypti embryogenesis.	the mosquito a. aegypti is vector of dengue and other viruses. new methods of vector control are needed and can be achieved by a better understanding of the life cycle of this insect. embryogenesis is a part of a. aegypty life cycle that is poorly understood. in insects in general and in mosquitoes in particular energetic metabolism is well studied during oogenesis, when the oocyte exhibits fast growth, accumulating carbohydrates, lipids and proteins that will meet the regulatory and metabolic n ...	2010	20184739
species composition and natural infectivity of anthropophilic anopheles (diptera: culicidae) in the states of córdoba and antioquia, northwestern colombia.	malaria is a serious health problem in the states of córdoba and antioquia, northwestern colombia, where 64.4% of total colombian cases were reported in 2007. because little entomological information is available in this region, the aim of this work was to identify the anopheles species composition and natural infectivity of mosquitoes distributed in seven localities with highest malaria transmission. a total of 1,768 anopheles mosquitoes were collected using human landing catches from march 200 ...	2009	20140372
occurrence of apocrine secretion in the larval gut epithelial cells of aedes aegypti l., anopheles albitarsis lynch-arribálzaga and culex quinquefasciatus say (diptera: culicidae): a defense strategy against infection by bacillus sphaericus neide?	cell hypertrophy was the first reaction of the gut epithelial cells of aedes aegypti (l.), anopheles albitarsis (lynch-arribálzaga) and culex quinquefasciatus (say) to the toxins of strains 2362 and s1116 of bacillus sphaericus, as cells had an increase of intracellular secretory activity. soon after the cell hypertrophy developed, vesicles were formed at the cell apical portion, which detached with the plasma membrane, characterizing a type of apocrine secretion like. the first pathway of conta ...	2009	19943010
the anopheles albitarsis complex with the recognition of anopheles oryzalimnetes wilkerson and motoki, n. sp. and anopheles janconnae wilkerson and sallum, n. sp. (diptera: culicidae).	the anopheles (nyssorhynchus) albitarsis complex includes six species: an. albitarsis, anopheles oryzalimnetes wilkerson and motoki, n. sp., anopheles marajoara, anopheles deaneorum, anopheles janconnae wilkerson and sallum, n. sp. and an. albitarsis f. except for an. deaneorum, species of the complex are indistinguishable when only using morphology. the problematic distinction among species of the complex has made study of malaria transmission and ecology of an. albitarsis s.l. difficult. conse ...	2009	19876554
species composition and distribution of adult anopheles (diptera: culicidae) in panama.	anopheles (diptera: culicidae) species composition and distribution were studied using human landing catch data over a 35-yr period in panama. mosquitoes were collected from 77 sites during 228 field trips carried out by members of the national malaria eradication service. fourteen anopheles species were identified. the highest average human biting rates were recorded from anopheles (nyssorhynchus) albimanus (wiedemann) (9.8 bites/person/night) and anopheles (anopheles) punctimacula (dyar and kn ...	2008	18826025
embryonic desiccation resistance in aedes aegypti: presumptive role of the chitinized serosal cuticle.	one of the major problems concerning dengue transmission is that embryos of its main vector, the mosquito aedes aegypti, resist desiccation, surviving several months under dry conditions. the serosal cuticle (sc) contributes to mosquito egg desiccation resistance, but the kinetics of sc secretion during embryogenesis is unknown. it has been argued that mosquito sc contains chitin as one of its components, however conclusive evidence is still missing.	2008	18789161
molecular phylogeny of neotropical anopheles (nyssorhynchus) albitarsis species complex (diptera: culicidae).	a phylogeny was reconstructed for four species belonging to the neotropical anopheles (nyssorhynchus) albitarsis complex using partial sequences from the mitochondrial cytochrome oxidase i (coi) and nadh dehydrogenase 4 (nd4) genes and the ribosomal dna its2 and d2 expansion region of the 28s subunit. the basis for initial characterization of each member of the complex was by correlated random amplification of polymorphic dna-polymerase chain reaction (rapd-pcr) markers. analyses were carried ou ...	0	18079976
affinity and diversity indices for anopheline immature forms.	as for the entire amazon region, malaria continues to be a major health public problem in roraima that presented an annual parasitic index of 85.4 in 2005, the highest in brazil. information on anopheline breeding sites is an essential component in malaria control strategies. aiming to contribute to the limited knowledge on anopheline immature forms in roraima, collections and breeding site observations were performed in 10 breeding sites around the capital city boa vista. collections were carri ...	2007	18026638
constant temperature and time period effects on anopheles gambiae egg hatching.	anopheles gambiae giles sensu stricto (diptera: culicidae) egg development and its relation to environmental parameters is an understudied aspect of vector biology. although several studies have illustrated the dramatic effects of temperature on egg development, egg hatching dynamics remain unclear. the objective of this study was to expose an. gambiae eggs to various temperatures for different lengths of time and determine the impact on egg development and hatching count. batches of mosquito eg ...	2007	17847843
parity and age composition for anopheles darlingi root (diptera: culicidae) and anopheles albitarsis lynch-arribálzaga (diptera: culicidae) of the northern amazon basin, brazil.	parity and age composition for anopheles darlingi and anopheles albitarsis in the northern amazon basin, brazil, were investigated. anopheline ovaries and ovarioles were examined in order to determine whether hourly and seasonal parity status for the vectors an. albitarsis and an. darlingi would vary in two different landscapes (forest and savanna/forest) where malaria is endemic in the northern amazon basin. a total of 1,199 anophelines (535 an. darlingi and 664 an. albitarsis) was dissected fo ...	2007	17633426
cytogenetic study of anopheles albitarsis (diptera: culicidae) by c-banding and in situ hybridization.	the c-banding pattern and the size and location of the nucleolar organizer regions (nors) are described for the first time in brazilian populations of anopheles (nyssorhynchus) albitarsis sensu lato. c-banding revealed variation in the size of the centromeric heterochromatic blocks in autosomal chromosomes and in the acrocentric (x) and puntiform (y) sex chromosomes. fluorescence in situ hybridization showed that the nors were located in the pericentromeric region of the sex (xx/xy) chromosomes ...	2006	17362336
habitat suitability mapping of anopheles darlingi in the surroundings of the manso hydropower plant reservoir, mato grosso, central brazil.	hydropower plants provide more than 78 % of brazil's electricity generation, but the country's reservoirs are potential new habitats for main vectors of malaria. in a case study in the surroundings of the manso hydropower plant in mato grosso state, central brazil, habitat suitability of anopheles darlingi was studied. habitat profile was characterized by collecting environmental data. remote sensing and gis techniques were applied to extract additional spatial layers of land use, distance maps, ...	2007	17343728
seasonal allergic dermatitis in sheep in southern brazil caused by culicoides insignis (diptera: ceratopogonidae).	the incidence of seasonal dermatitis was studied in a hampshire down flock on a farm in southern brazil. epidemiological data, clinical signs and macroscopic pathology were obtained by visiting the farm. histological lesions were studied in skin biopsies of affected sheep. biting insects were collected from january to april 2005 in an attempt to identify the etiological agent of the disease. disease prevalence was 40%; the age of the affected animals was variable. disease occurred from december ...	2007	17127007
major chorion proteins and their crosslinking during chorion hardening in aedes aegypti mosquitoes.	the chorion of aedes aegypti eggs undergoes a hardening process following oviposition and individual chorion proteins become insoluble thereafter. our previous studies determined that peroxidase-catalyzed chorion protein crosslinking and phenoloxidase-mediated chorion melanization are primarily responsible for the formation of a hardened, desiccation resistant chorion in a. aegypti eggs. to gain further understanding of peroxidase- and phenoloxidase-catalyzed biochemical processes during chorion ...	2006	17098170
cryptic species in the anopheles (nyssorhynchus) albitarsis (diptera: culicidae) complex: incongruence between random amplified polymorphic dna-polymerase chain reaction identification and analysis of mitochondrial dna coi gene sequences.	random amplified polymorphic dna (rapd) diagnostic bands are one tool used to differentiate cryptic mosquito species in the anopheles albitarsis complex. monophyly of four species (a. albitarsis lynch-arribálzaga, a. albitarsis b, a. deaneorum rosa-freitas, and a. marajoara galvão & damasceno) currently identified with the rapd technique was assessed using sequences of the cytochrome oxidase i (coi) mitochondrial dna (mtdna) gene. maximum parsimony, maximum likelihood, and bayesian analyses supp ...	0	17082822
[anophelines in belém, pará, brazil: current and retrospective data].	we present the results of anopheline captures in belém, pará, brazil, from 1995-2004, and a comparison with captures from 1930-1999. in the earlier period, 20 species were identified: anopheles albitarsis s.l., an. aquasalis, an. argyritarsis, an. braziliensis, an. darlingi, an. eiseni, an. evansae, an. galvaoi, an. intermedius, an. kompi, an. mediopunctatus, an. nimbus, an. nuneztovari, an. oswaldoi, an. peryassui, an. punctimacula, an. shannoni, an. strodei, an. thomasi, and an. triannulatus. ...	2006	16832529
the importance of anopheles albitarsis e and an. darlingi in human malaria transmission in boa vista, state of roraima, brazil.	in several districts of boa vista, state of roraima, brazil we found anopheles (nyssorhynchus) albitarsis e to be the primary vector of human malaria parasites, and during 2001-2002 it was significantly more abundant than an. darlingi (p < 0.001). other species sampled were an. (nys.) braziliensis, an. (ano.) peryassui, an. (nys.) nuneztovari, an. (nys.) oswaldoi s.l., and an. (nys.) triannulatus. as determined by the elisa technique an. darlingi had a higher overall infection rate (2.1%) compar ...	2006	16830709
tetrahymenidae infection in mosquito populations in a malaria-endemic region of the amazon.	in a field survey performed in a malaria-endemic region of northern amazon, brazil, we encountered ciliate protozoa of the family tetrahymenidae infecting adults and larvae of the following mosquito species: culex sp., anopheles albitarsis l.s., anopheles strodei, anopheles mattogrossensis, anopheles darlingi, and anopheles oswaldoi l.s. based on morphological features and life style, we have tentatively identified the parasite as lambornella sp. the association appears pathogenic for the mosqui ...	2006	16497321
karyotype of brazilian anopheles albitarsis sensu lato (diptera:culicidae).	anopheles (nyssorhynchus) albitarsis sensu lato is an important malaria vector in brazil, especially in the brazilian amazon region. chromosome preparations of fourth-instar larvae of a. albitarsis from iranduba and coari (am) and ilha comprida (sp) were analyzed for karyotype determination and to improve cytogenetic identification of this species. anopheles albitarsis possesses 2n = 6 chromosomes, with two pairs (submetacentric and metacentric) of autosomes and one pair of sex chromosomes, with ...	2005	16475113
anopheles albitarsis s.l. (diptera: culicidae) survivorship and density in a rice irrigation area of the state of são paulo, brazil.	collections of anopheles albitarsis arribalzaga s.l. were conducted from may 1996 to june 2000 to estimate the human biting rate, and mark-release-recapture experiments were carried out to estimate the daily survival probability. the density of an. albitarsis females showed a marked seasonal variation, with peaks as high as 629 individuals per human per night in the wet season. the low daily survival probability of 0.61 is an important factor limiting the vectorial capacity of an. albitarsis s.l ...	2004	15535635
analysis of reproductive isolation between sibling species anopheles albitarsis sensu stricto and anopheles deaneorum, two malaria vectors belonging to the albitarsis complex (diptera: culicidae).	complexes of sibling species are common among mosquitoes, and their existence within vector species can have important epidemiological consequences. anopheles albitarsis sensu stricto and anopheles deaneorum rosa-freitas are two putative vectors of malaria parasites belonging to the albitarsis species complex (diptera: culicidae). using an induced mating technique, we studied the reproductive isolation between these two closely related species and their reciprocal hybrids. evidence for hybrid ma ...	2004	15535617
[information concerning the anopheles albitarsis domesticus, one of the transmitters of malaria in brazil (diptera, culicidae)].		1950	15405237
adaptation of a south american malaria vector to laboratory colonization suggests faster-male evolution for mating ability.	anopheles (nyssorhynchus) albitarsis (diptera: culicidae) is one of the very few south american mosquito vectors of malaria successfully colonized in the laboratory. these vectors are very hard to breed because they rarely mate in artificial conditions. a few years ago a free-mating laboratory colony of an. albitarsis sensu stricto was established after about 30 generations of artificial-mating. to begin to understand the process of adaptation of these malaria vectors to the laboratory we have c ...	2004	15132759
observations on the flight and longevity in nature of anopheles albitarsis domesticus.		1950	14795210
two digestive trypsins occur in three species of neotropical anophelines.	trypsin activity increases in the midgut of anopheles aquasalis, anopheles albitarsis, and anopheles darlingi after a bloodmeal. the activity returns to basal levels at the time the blood is completely digested. affinity chromatography, reversed-phase high performance liquid chromatography (hplc), and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) were used to sequentially purify the mosquito trypsins found in the midguts at 24 h after feeding. aminoterminal sequencing of t ...	2003	14765682