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in vitro and in vivo exploration of palmitic acid from synechococcus elongatus as an antibiofilm agent on the survival of artemia franciscana against virulent vibrios.biofilm formation of vibrio spp. has been demonstrated as a potentially important mechanism contributing antibiotic treatment failure in aquaculture. in the present study, the effect of palmitic acid (pa) identified from synechococcus elongatus was assessed for the inhibition of quorum sensing (qs) regulated biofilm formation in aquatic bacterial pathogens. the biofilm inhibitory concentration (bic) of pa against vibrio spp. was found to be 100µgml(-1). in this concentration, pa exhibited a sign ...201728887169
[evaluation of the toxicity of basidiomycota fungi on the hatching of artemia franciscana cysts].the consumption of wild mushrooms has increased in recent years. however, not all mushrooms are edible and some of them may cause poisoning. therefore, their toxicity needs to be studied. artemia franciscana is a crustacean used in toxicity tests including toxins of fungi.201728830736
effects of polystyrene microbeads in marine planktonic crustaceans.plastic debris accumulates in the marine environment, fragmenting into microplastics (mp), causing concern about their potential toxic effects when ingested by marine organisms. the aim of this study was to verify whether 0.1µm polystyrene beads are likely to trigger lethal and sub-lethal responses in marine planktonic crustaceans. mp build-up, mortality, swimming speed alteration and enzyme activity (cholinesterases, catalase) were investigated in the larval stages of amphibalanus amphitrite ba ...201728750293
long-term toxicity of surface-charged polystyrene nanoplastics to marine planktonic species dunaliella tertiolecta and artemia franciscana.plastic pollution has been globally recognized as a critical issue for marine ecosystems and nanoplastics constitute one of the last unexplored areas to understand the magnitude of this threat. however, current difficulties in sampling and identifying nano-sized debris make hard to assess their occurrence in marine environment. polystyrene nanoparticles (ps nps) are largely used as nanoplastics in ecotoxicological studies and although acute exposures have been already investigated, long-term tox ...201728644993
enzyme differentiation and phylogeny in clarkia franciscana, c. rubicunda and c. amoena. 197328564780
terminalia ferdinandiana exell. extracts inhibit the growth of body odour-forming bacteria.terminalia ferdinandiana extracts are potent growth inhibitors of many bacterial pathogens. they may also inhibit the growth of malodour-producing bacteria and thus be useful deodorant components, although this is yet to be tested.201728488331
defense systems in developing artemia franciscana nauplii and their modulation by probiotic bacteria offer protection against a vibrio anguillarum challenge.the alterations of immune responses of artemia franciscana nauplii as a function of culture time and after a challenge with the pathogen vibrio anguillarum were studied. the effect of the administration of the probiotic bacteria bacillus subtilis, lactobacillus plantarum and lactococcus lactis either alone or in combination with the pathogen was evaluated. the activity of the antioxidant enzymes superoxide dismutase (sod), glutathione reductase (gred), glutathione transferase (gst) and phenoloxi ...201728478257
determination of the optimal enrichment artemia franciscana with a synbiotic combination of probiotics pediococcus acidilactici and prebiotic fructooligosaccharide.in this study the optimal amount of enriching artemia franciscana with a synbiotic combination of pediococcus acidilactici and fructooligosaccharide (fos( was investigated. the experiment was conducted in a completely randomized design in ten treatments: multi-level probiotics p. acidilactici (1×10(9), 1×10(8) and 1× 10(7) cfu per l) and multi-level enriched prebiotic fos (1, 2 and 5) g per l of solution and control. to evaluate the enrichment of adult artemia with each treatment, sampling was p ...201728473897
effect of methylparaben in artemia franciscana.in this study, the toxicity of methylparaben (mep) an emerging contaminant, was analysed in the sexual species artemia franciscana, due to its presence in coastal areas and marine saltworks in the mediterranean region. the acute toxicity (24h-lc50) of mep in nauplii was tested and its chronic effect (9days) evaluated by measuring survival and growth under two sublethal concentrations (0.0085 and 0.017mg/l). also, the effect on several key enzymes involved in: antioxidant defences (catalase (cat) ...201728428009
social sounds produced by franciscana dolphins, pontoporia blainvillei (cetartiodactyla, pontoporiidae).franciscana dolphin (pontoporia blainvillei) whistles were documented for the first time during 2003-2013 in babitonga bay estuary, south brazil, together with burst pulses. recordings were made from small boats under good sea conditions, and recording equipment that allowed analysis of sounds up to 96 khz. the recordings were made in the presence of 2-31 franciscana dolphins. during 23 h and 53 min, 90 whistles and 51 burst pulse series were recorded. although guiana dolphins (sotalia guianensi ...201728372148
prey species of franciscana pontoporia blainvillei as paratenic hosts of helminths.the distribution of franciscana pontoporia blainvillei gervais & d'orbigny, 1884, is restricted to the estuarine and coastal waters of the southwestern atlantic. the diet of this dolphin is composed of fishes, squids, and shrimps, many of which harbor helminths that may infect franciscana. larval forms of the trematode synthesium pontoporiae and the acanthocephalan bolbosoma turbinella have been recorded in franciscana; however, they have not yet been identified in any of the prey species of thi ...201728322211
food-web modification by an invertebrate predator in the great salt lake (usa).during unusually wet years the salinity of the great salt lake (utah) decreased from above 100 g/l to 50 g/l. this allowed the predaceous insect trichocorixa verticalis to invade the pelagic region of the lake and reach a mean summer density of 52/m(3). concurrent changes in the pelagic ecosystem were: a decrease in the dry biomass of the previously dominant filter-feeding brine shrimp artemia franciscana from 720 to 2 mg/m(3), the invasion of three other zooplankton taxa, a 10 × decrease in com ...199228312870
segmentation in the crustacean artemia: engrailed staining studied with an antibody raised against the artemia protein.we have studied the process of post-embryonic segmentation in the anostracan crustacean artemia franciscana using a specific antibody raised against the engrailed protein of this organism. three cephalic segments are specified during embryonic development, before larval hatching, whilst trunk (thoracic) segmentation begins after the first stage free-swimming nauplius larva has emerged from the dormant cyst. thus, cephalic and trunk segmentation seem to be at least in part independent and superim ...199628306093
cell-specific endopolyploidy in developing artemia.cells in developing artemia franciscana sfb demonstrated tissue-specific differences in dna content, as determined by fluorescence intensity of bisbenzimide-stained nuclei and by nuclear area. the general epidermis comprised proliferating diploid (2c) cells. the setal cells had 4c-8c dna content and did not divide during the first two instars. salt gland cells were polyploid (>8c) and also did not undergo mitosis. neural cells in the brain were diploid and were replicating. cells in the thorax r ...198828305474
enhanced resistance against vibrio harveyi infection by carvacrol and its association with the induction of heat shock protein 72 in gnotobiotic artemia franciscana.induction of hsp72 is a natural response of stressed organisms that protects against many insults including bacterial diseases in farm (aquatic) animals. it would therefore be of great health benefit to search for natural compounds that are clinically safe yet able to induce hsp72 in animals. the phenolic compound carvacrol, an approved food component, had been shown in in vitro study to act as a co-inducer of hsp72, enhancing hsp72 production only in combination with a bona fide stress compared ...201728303510
identification and characterization of a masculinizer (masc) gene involved in sex differentiation in artemia.the sex of relatively primitive animals such as invertebrates is mostly determined by environmental factors and chromosome ploidy. heteromorphic chromosomes may also play an important role, as in the zw system in lepidopterans. however, the mechanisms of these various sex determination systems are still largely undefined. in the present study, a masculinizer gene (ar-masc) was identified in the crustacean artemia franciscana kellogg 1906. sequence analysis revealed that the 1140-bp full-length o ...201728300613
influence of surfactants and humic acids on artemia franciscana's embryonic phospho-metabolite profile as measured by (31)p nmr.surfactants, such as triton x-100 (tx-100), cetylpyridinium chloride (cpc), and sodium dodecyl sulfate (sds) are known to be toxic to artemia franciscana (artemia) - an organism, frequently used to monitor the health of the aquatic environment. the phospho-metabolite profile of a living organism is often indicative of imbalances that may have been caused by environmental stressors, such as surfactants. this study utilizes in vivo(31)p nmr to monitor temporal changes in the phospho-metabolite pro ...201728285196
molecular phylogeny and toxicity of harmful benthic dinoflagellates coolia (ostreopsidaceae, dinophyceae) in a sub-tropical marine ecosystem: the first record from hong kong.coolia are marine benthic dinoflagellates which are globally distributed and potentially toxic. this study provides the first investigation of species diversity and toxicity assessment of coolia in hong kong waters. fifty-one strains of four coolia species, including c. malayensis, c. canariensis, c. tropicalis, and c. palmyrensis, were isolated from twelve sub-tidal habitats, and identified phylogenetically using 28s rdna sequences. exposure experiments (48-hour) demonstrated that the algal lys ...201728139234
variation of the interphase heterochromatin in artemia (crustacea, anostraca) of the americas is related to changes in nuclear size and ionic composition of hipersaline habitats.the populations of artemia (or brine shrimp) from the americas exhibit a wide variation in the amount of interphase heterochromatin. there is interest in understanding how this variation affects different parameters, from the cellular to the organismal levels. this should help to clarify the ability of this organism to tolerate brine habitats regularly subject to strong abiotic changes. in this study, we assessed the amount of interphase heterochromatin per nucleus based on chromocenter number ( ...201728099570
patterns of population structure at microsatellite and mitochondrial dna markers in the franciscana dolphin (pontoporia blainvillei).the franciscana dolphin, pontorporia blainvillei, is an endemic cetacean of the atlantic coast of south america. its coastal distribution and restricted movement patterns make this species vulnerable to anthropogenic factors, particularly to incidental bycatch. we used mitochondrial dna control region sequences, 10 microsatellites, and sex data to investigate the population structure of the franciscana dolphin from a previously established management area, which includes the southern edge of its ...201628035267
yellow-emitting carbon nanodots and their flexible and transparent films for white leds.we report carbon nanodots that can be utilized as effective color converting phosphors for the production of white light-emitting diodes (leds). blue-excitable and yellow-emitting carbon nanodots, functionalized with 3-(imidazolidin-2-on-1-yl)propylmethyldimethoxysilane (ipmds)-derived moieties (is-cds), are synthesized by a novel one-pot reaction in which the products from the initial reaction occurring between urea and 3-(2-aminoethylamino)propylmethyl-dimethoxysilane (aepmds) are further trea ...201627934198
potential effects of tio2 nanoparticles and ticl4 in saltwater to phaeodactylum tricornutum and artemia franciscana.nanosized titanium dioxide (ntio2) is widespread in many commercial products and several authors investigated its ecotoxicity effects focusing mainly on freshwater environments. data on saltwater species are still lacking or present contradicting results. we compared for the first time the toxicity of ticl4 and ntio2 considering standard toxicity tests with microalgae phaeodactylum tricornutum (growth inhibition test, 1.8-90mg/l) and crustacean artemia franciscana (mortality test, 0.5-64mg/l). f ...201727913027
metals as chemical tracers to discriminate ecological populations of threatened franciscana dolphins (pontoporia blainvillei) from argentina.franciscana dolphins are the most impacted small cetacean in the southwestern atlantic ocean, classified as vulnerable a3d by iucn. essential (fe, mo, mn, cr, ni, co) and non-essential (ag, pb, sn) trace elements (tes) were measured in liver, kidney, and brain samples of by-catch franciscana dolphins that were living in estuarine (n = 21) and marine (n = 21) habitats (1) to assess whether tes posed a threat and (2) to evaluate the suitability of tes for discriminating ecological populations of t ...201727909926
evaluation of a battery of marine species-based bioassays against raw and treated municipal wastewaters.the present study evaluates a battery of marine species-based bioassays against chemically characterized municipal wastewater samples (raw and wwtp treated). we estimated dunaliella tertiolecta growth rate inhibition (24-96h ic50 values), artemia franciscana immobilization (24h lc50 values), mussel hemocytes viability and lipid peroxidation enhancement (in terms of neutral red retention assay/nrrt and malondialdehyde/mda content, respectively) in influent- and wwtp effluent-treated species. we f ...201727676080
rotenone decreases hatching success in brine shrimp embryos by blocking development: implications for zooplankton egg banks.while many zooplankton species recover quickly after the treatment of water resources with the piscicide, rotenone, some fail to reach pretreatment population density or, in rare cases, do not reappear at all. the variable impact of rotenone on zooplankton populations could stem from differences in the capacity of species to switch entirely to anaerobic catabolic pathways in the presence of rotenone, which blocks mitochondrial electron transport. alternatively, variable responses among species c ...201727655396
non-lethal heat shock increases tolerance to metal exposure in brine shrimp.pollution and temperature increase are two of the most important stressors that aquatic organisms are facing. exposure to elevated temperatures and metal contamination both induce heat shock proteins (hsps), which may thus be involved in the induced cross-tolerance in various organisms. this study aimed to test the hypothesis that exposure to a non-lethal heat shock (nlhs) causes an increased tolerance to subsequent metal exposure. using gnotobiotic cultures of the brine shrimp artemia francisca ...201627619211
mercury, selenium and stable isotopes in four small cetaceans from the southeastern brazilian coast: influence of feeding strategy.dolphins are good bioindicators of the contamination status of marine ecosystems, since their dietary and habitat plasticity in both coastal and offshore ecotypes provide information on the trace elements levels originated from natural and anthropogenic sources. in this context, this study aimed to investigate provides mercury (hg), selenium (se) levels, trophic ecology and feeding environments of four small cetaceans (tursiops truncatus, steno bredanensis, sotalia guianensis and pontoporia blai ...201627593348
arhsp40, a type 1 j-domain protein, is developmentally regulated and stress inducible in post-diapause artemia franciscana.upon diapause termination and exposure to favorable environmental conditions, cysts of the crustacean artemia franciscana reinitiate development, a process dependent on the resumption of metabolic activity and the maintenance of protein homeostasis. the objective of the work described herein was to characterize molecular chaperones during post-diapause growth of a. franciscana. an hsp40 complementary dna (cdna) termed arhsp40 was cloned and shown to encode a protein with an amino-terminal j-doma ...201627581971
functional role of native and invasive filter-feeders, and the effect of parasites: learning from hypersaline ecosystems.filter-feeding organisms are often keystone species with a major influence on the dynamics of aquatic ecosystems. studies of filtering rates in such taxa are therefore vital in order to understand ecosystem functioning and the impact of natural and anthropogenic stressors such as parasites, climate warming and invasive species. brine shrimps artemia spp. are the dominant grazers in hypersaline systems and are a good example of such keystone taxa. hypersaline ecosystems are relatively simplified ...201627560978
the total and mitochondrial lipidome of artemia franciscana encysted embryos.encysted embryos (cysts) of the crustacean artemia franciscana exhibit enormous tolerance to adverse conditions encompassing high doses of radiation, years of anoxia, desiccation and extreme salinity. so far, several mechanisms have been proposed to contribute to this extremophilia, however, none were sought in the lipid profile of the cysts. here in, we used high resolution shotgun lipidomics suited for detailed quantitation and analysis of lipids in uncharacterized biological membranes and sam ...201627542539
surfactant toxicity to artemia franciscana and the influence of humic acid and chemical composition.surfactants can be extremely toxic to aquatic species and are introduced to the environment in a variety of ways. it is thus important to understand how other environmental constituents, in this case humic acids (has), may alter the toxicity of anthropogenic surfactants. hatching and mortality assays of artemia franciscana were performed for three different toxic surfactants: triton x-100 (tx-100, non-ionic), cetylpyridinium chloride (cpc, cationic), and sodium dodecyl sulfate (sds, anionic). hu ...201727453688
three new species and a new record of smicridea mclachlan 1871 (trichoptera: hydropsychidae) from minas gerais state, brazil.three new species of smicridea mclachlan 1871 are described and illustrated based on specimens collected in parque nacional da serra da canastra and its surrounding area, minas gerais state, southeastern brazil. smicridea (rhyacophylax) necator sp. nov. possibly belongs to the s. (r.) dispar group, but its placement in this group is weakly supported; s. (smicridea) franciscana sp. nov. belongs to the s. (s.) nigripennis group based on morphology of the phallus; s. (s.) sepala sp. nov. is a membe ...201627394831
cryopreservation of lipid bilayers by lea proteins from artemia franciscana and trehalose.the capacity of late embryogenesis abundant (lea) proteins and trehalose to protect liposomes against freezing-induced damage was examined by measuring the leakage of 5(6)-carboxyfluorescein (cf). liposomes were prepared to simulate the lipid compositions of the inner leaflet of the plasma membrane, outer mitochondrial membrane (omm), and inner mitochondrial membrane (imm). two recombinant lea proteins belonging to group 3 (afrlea2 and afrlea3m) were expressed and purified from embryos of artemi ...201627393243
beak deviations in the skull of franciscana dolphins pontoporia blainvillei from argentina.the franciscana dolphin pontoporia blainvillei is characterized by a long rostrum, a feature that is shared with the families formerly classified as river dolphins (pontoporiidae, platanistidae, iniidae, lipotidae). although there are occasional reports on the existence of beak deformations, very little published information exists describing this process. the object of the present study was to describe and quantify the beak anomalies of franciscana dolphins from the coastal waters of argentina. ...201627304865
high performance liquid chromatography-mass spectrometry analysis of high antioxidant australian fruits with antiproliferative activity against cancer cells.high antioxidant capacities have been linked to the treatment and prevention of several cancers. recent reports have identified several native australian fruits with high antioxidant capacities. despite this, several of these species are yet to be tested for anticancer activity.201627279705
biochemical and toxicological effects of organic (herbicide primextra(®) gold tz) and inorganic (copper) compounds on zooplankton and phytoplankton species.in europe, mainly in the mediterranean region, an intensive usage of pesticides was recorded during the past 30 years. according to information from agricultural cooperatives of the mondego valley (figueira da foz, portugal), primextra(®) gold tz is the most used herbicide in corn crop fields and one of the 20 best-selling herbicides in portugal. copper is mainly used in pesticide formulations. this study aims to determine the ecotoxicological and biochemical (namely fatty acid profiles) effects ...201627239776
the role of metallothioneins, selenium and transfer to offspring in mercury detoxification in franciscana dolphins (pontoporia blainvillei).the concentrations of mercury (hg), selenium (se) and metallothioneins (mt) were evaluated in fetuses, calves, juveniles and adults of the endangered coastal franciscana dolphin (pontoporia blainvillei) from argentina. mercury concentrations varied among analyzed tissues (liver, kidney, muscle and brain), with liver showing the higher concentrations in all specimens. an age-dependent accumulation was found in liver, kidney and brain. no significant relationship between hg and mt concentrations w ...201627210558
impacts of harvesting on brine shrimp (artemia franciscana) in great salt lake, utah, usa.selective harvesting can cause evolutionary responses in populations via shifts in phenotypic characteristics, especially those affecting life history. brine shrimp (artemia franciscana) cysts in great salt lake (gsl), utah, usa are commercially harvested with techniques that select against floating cysts. this selective pressure could cause evolutionary changes over time. our objectives are to (1) determine if there is a genetic basis to cyst buoyancy, (2) determine if cyst buoyancy and nauplii ...201627209783
maladaptive sex ratio adjustment in the invasive brine shrimp artemia franciscana.sex allocation theory is often hailed as the most successful area of evolutionary theory due to its striking success as a predictor of empirical observations [1]. most naturally occurring sex ratios can be explained by the principle of equal investment in the sexes [2-4] or by cases of "extraordinary" sex allocation [5]. deviations from the expected sex ratio are often correlated with weak selection or low environmental predictability (e.g., [6, 7]); true cases of aberrant sex allocation are sur ...201627185556
the effects of feeding with synbiotic (pediococcus acidilactici and fructooligosaccharide) enriched adult artemia on skin mucus immune responses, stress resistance, intestinal microbiota and performance of angelfish (pterophyllum scalare).the aim of this study was to evaluate the effects of feeding on synbiotic (pediococcus acidilactici and fructooligosaccharide) enriched adult artemia franciscana on skin mucus immune responses, stress resistance, intestinal microbiota and growth performance of angelfish (pterophyllum scalare). three hundred and sixty fish with initial weight 3.2 ± 0.13 g were randomly divided into twelve aquaria (50 l) assigned to four groups in triplicates. fish were fed for 7 weeks with dietary treatments, inc ...201627150050
gamma radiation reduced toxicity of azoxystrobin tested on artemia franciscana.fungicide azoxystrobin toxicity was monitored by means of a 96-h biotest with artemia franciscana nauplius stages after exposure to solutions with concentrations of 0.2, 0.4, 0.6 and 0.8 mg l(-1) irradiated with (60)co gamma radiation with doses of 1, 2.5, 5 and 10 kgy. the effects of ionization radiation on azoxystrobin toxicity were mainly manifested by a statistically significant reduction of lethality after 72- and 96-h exposure. a maximum reduction of lethality of 72 % was achieved using do ...201627107585
remediation of mercury contaminated saltwater with functionalized silica coated magnetite nanoparticles.the study aimed to evaluate the efficiency of dithiocarbamate functionalized silica coated magnetite nanoparticles (nps) for hg decontamination of saltwater either contaminated with hg alone or with as and cd. for this, the residual levels of hg in seawater were assessed and hg-contaminated or hg+as+cd-contaminated seawater toxicity to aquatic biota, before and after the sorption process, was compared. the results showed that under highly competitive conditions (water salts, cd and as), the remo ...201627039062
mercury species, selenium, metallothioneins and glutathione in two dolphins from the southeastern brazilian coast: mercury detoxification and physiological differences in diving capacity.in the present study, the concentration of trace elements, total mercury (hg) and selenium (se) and mercury forms (mehg, hginorg and hgse) in the vulnerable coastal dolphins pontoporia blainvillei and sotalia guianensis were appraised and compared, using metallothioneins (mt) and glutathione (gsh) as biomarkers for trace element exposure. the trace element concentrations varied between muscle and liver tissues, with liver of all dolphin specimens showing higher hg and se concentrations than thos ...201627038210
may arsenic pollution contribute to limiting artemia franciscana invasion in southern spain?limited information exists regarding the complex interactions between biological invasions, pollution, and climate change. most studies indicate that pollution tends to favor invasive species. here, we provide evidence that arsenic (as) pollution may have a role in limiting the invasion of the exotic brine shrimp artemia franciscana. we tested as toxicity in natural populations of artemia parthenogenetica (a native taxon) and a. franciscana from localities in southern spain with differing degree ...201626925327
a case study to optimise and validate the brine shrimp artemia franciscana immobilisation assay with silver nanoparticles: the role of harmonisation.brine shrimp artemia sp. has been recognised as an important ecotoxicity and nanotoxicity test model organism for salt-rich aquatic environments, but currently there is still no harmonised testing protocol which would ensure the comparable results for hazard identification. in this paper we aimed to design the harmonised protocol for nanomaterial toxicity testing using artemia franciscana and present a case study to validate the protocol with silver nanoparticles (agnps). we (i) revised the exis ...201626895539
assessing estuarine quality: a cost-effective in situ assay with amphipods.in situ assays based on feeding depression can be powerful ecotoxicological tools that can link physiological organism-level responses to population and/or community-level effects. amphipods are traditional target species for toxicity tests due to their high sensitivity to contaminants, availability in the field and ease of handling. however, cost-effective in situ assays based on feeding depression are not yet available for amphipods that inhabit estuarine ecosystems. the aim of this work was t ...201626874320
assessing disease and mortality among small cetaceans stranded at a world heritage site in southern brazil.cetaceans are considered environmental sentinels and their health often reflects either anthropogenic or natural spatio-temporal disturbances. this study investigated the pathological findings and mortality of small cetaceans with the aim of detecting hazards and monitoring health trends in a high-biodiversity area. between 2007 and 2012, 218 stranded cetaceans were recorded on the paraná coast, southern brazil. fifty-seven (26.1%) of these animals, including 50 sotalia guianensis, 2 pontoporia ...201626871703
long-term seasonal and interannual patterns of marine mammal strandings in subtropical western south atlantic.understanding temporal patterns of marine mammal occurrence is useful for establishing conservation strategies. we used a 38 yr-long dataset spanning 1976 to 2013 to describe temporal patterns and trends in marine mammal strandings along a subtropical stretch of the east coast of south america. this region is influenced by a transitional zone between tropical and temperate waters and is considered an important fishing ground off brazil. generalized additive models were used to evaluate the tempo ...201626814667
salt sensitivity of the morphometry of artemia franciscana during development: a demonstration of 3d critical windows.a 3d conceptual framework of 'critical windows' was used to examine whether the morphometry of artemia franciscana is altered by salinity exposure during certain key periods of development. artemia franciscana were hatched at 20 ppt (designated control salinity) and were then exposed to 10, 30, 40 or 50 ppt either chronically (days 1-15) or only on days 1-6, 7-9, 10-12 or 13-15. on day 15, maturity was assessed and morphometric characteristics, including mass, total body length, tail length and ...201626685168
an interlaboratory comparison of nanosilver characterisation and hazard identification: harmonising techniques for high quality data.within the fp7 eu project nanovalid a consortium of six partners jointly investigated the hazard of silver nanoparticles (agnps) paying special attention to methodical aspects that are important for providing high-quality ecotoxicity data. laboratories were supplied with the same original stock dispersion of agnps. all partners applied a harmonised procedure for storage and preparation of toxicity test suspensions. altogether ten different toxicity assays with a range of environmentally relevant ...201626638016
pcb and pbde levels in a highly threatened dolphin species from the southeastern brazilian coast.in the northern coast of rio de janeiro state is located the major urban centers of the oil and gas industry of brazil. the intense urbanization in recent decades caused an increase in human use of the coastal areas, which is constantly impacted by agricultural, industrial and wastewater discharges. franciscana dolphin (pontoporia blainvillei) is a small cetacean that inhabits coastal regions down to a 30 m depth. this species is considered the most threatened cetacean in the western south atlan ...201626552541
nodularia spumigena peptides--accumulation and effect on aquatic invertebrates.thus far, the negative effects of nodularia spumigena blooms on aquatic organisms have been mainly attributed to the production of the hepatotoxic nodularin (nod). in the current work, the accumulation of other n. spumigena metabolites in blue mussels and crustaceans, and their effect on thamnocephalus platyurus and artemia franciscana, were examined. the liquid chromatography-tandem mass spectrometry (lc-ms/ms) analyses provided evidence that both blue mussels collected after a cyanobacterial b ...201526529012
liposomes with diverse compositions are protected during desiccation by lea proteins from artemia franciscana and trehalose.intracellular accumulation of late embryogenesis abundant (lea) proteins and the disaccharide trehalose is associated with cellular desiccation tolerance in a number of animal species. two lea proteins from anhydrobiotic embryos of the brine shrimp artemia franciscana were tested for the ability to protect liposomes of various compositions against desiccation-induced damage in the presence and absence of trehalose. damage was assessed by carboxyfluorescein leakage after drying and rehydration. f ...201626518519
degradation and toxicity reduction of the endocrine disruptors nonylphenol, 4-tert-octylphenol and 4-cumylphenol by the non-ligninolytic fungus umbelopsis isabellina.nonylphenol (np), 4-tert-octylphenol (4-t-op) and 4-cumylphenol (4-cp) are pollutants that are known as endocrine disruptors mainly due to their estrogen-mimicking activity. these phenolic substances are used in a wide range of industrial and commercial applications. in the present study, biodegradation of tnp, 4-t-op and 4-cp using the non-ligninolytic fungus umbelopsis isabellina was investigated. after 12h of incubation, more than 90% of initially applied tnp, 4-t-op and 4-cp (25mgl(-1)) were ...201626492175
effects of rearing density on survival, growth, and development of the ladybird coleomegilla maculata in culture.our research focuses on developing techniques to rear ladybird beetles (coleoptera: coccinellidae). we evaluated the effects of rearing density on survival, growth, and development of coleomegilla maculata. the hypothesis that a low to moderate rearing density has limited or no effects on survival and development was tested. c. maculata first instars were reared to pupae at a density of 1, 5, 10, 15, or 20 individuals per arena (2.5 cm high, 9.0 cm diameter, and 159 cm3 volume) and fed powdered ...201526466904
does a change from whole to powdered food (artemia franciscana eggs) increase oviposition in the ladybird coleomegilla maculata?the limited availability of alternative foods to replace natural prey hinders cost-effective mass production of ladybird beetles for augmentative biological control. we compared the effects of powdered vs. whole artemia franciscana (a. franciscana) (brine shrimp) eggs with or without a dietary supplement on development and reproduction of coleomegilla maculata (c. maculata) (coleoptera: coccinellidae). we tested the hypotheses that (1) powdered a. franciscana eggs are more suitable than whole eg ...201526466902
study of model systems to test the potential function of artemia group 1 late embryogenesis abundant (lea) proteins.embryos of the brine shrimp, artemia franciscana, are genetically programmed to develop either ovoviparously or oviparously depending on environmental conditions. shortly upon their release from the female, oviparous embryos enter diapause during which time they undergo major metabolic rate depression while simultaneously synthesize proteins that permit them to tolerate a wide range of stressful environmental events including prolonged periods of desiccation, freezing, and anoxia. among the know ...201626462928
the gnotobiotic brine shrimp (artemia franciscana) model system reveals that the phenolic compound pyrogallol protects against infection through its prooxidant activity.the phenolic compound pyrogallol is the functional unit of many polyphenols and currently there has been a growing interest in using this compound in human and animal health owing to its health-promoting effects. the biological actions of pyrogallol moiety (and polyphenols) in inducing health benefitting effects have been studied; however, the mechanisms of action remain unclear yet. here, we aimed at unravelling the underlying mechanism of action behind the protective effects of pyrogallol agai ...201526459033
toxic heritage: maternal transfer of pyrethroid insecticides and sunscreen agents in dolphins from brazil.pyrethroids (pyr) and uv filters (uvf) were investigated in tissues of paired mother-fetus dolphins from brazilian coast in order to investigate the possibility of maternal transfer of these emerging contaminants. comparison of pyr and uvf concentrations in maternal and fetal blubber revealed franciscana transferred efficiently both contaminants to fetuses (f/m > 1) and guiana dolphin transferred efficiently pyr to fetuses (f/m > 1) different than uvf (f/m < 1). pyr and uvf concentrations in fet ...201526453834
nano-sized polystyrene affects feeding, behavior and physiology of brine shrimp artemia franciscana larvae.nano-sized polymers as polystyrene (ps) constitute one of the main challenges for marine ecosystems, since they can distribute along the whole water column affecting planktonic species and consequently disrupting the energy flow of marine ecosystems. nowadays very little knowledge is available on the impact of nano-sized plastics on marine organisms. therefore, the present study aims to evaluate the effects of 40nm anionic carboxylated (ps-cooh) and 50nm cationic amino (ps-nh2) polystyrene nanop ...201626422775
lethal and sublethal endpoints observed for artemia exposed to two reference toxicants and an ecotoxicological concern organic compound.swimming speed alteration and mortality assays with the marine crustacean artemia franciscana were carried out. ec50 and lc50 values after 24-48h exposures were calculated for two reference toxicants, copper sulphate pentahydrate (cuso4·5h2o) and sodium dodecyl sulphate (sds), and an ecotoxicological concern organic compound, diethylene glycol (deg). different end-points have been evaluated, in order to point out their sensitivity levels. the swimming speed alteration (ssa) was compared to morta ...201626344887
carbazole hydroxylation by the filamentous fungi of the cunninghamella species.nitrogen heterocyclic compounds, especially carbazole, quinolone, and pyridine are common types of environmental pollutants. carbazole has a toxic influence on living organisms, and the knowledge of its persistence and bioconversion in ecosystems is still not complete. there is an increasing interest in detoxification of hazardous xenobiotics by microorganisms. in this study, the ability of three filamentous fungi of the cunninghamella species to eliminate carbazole was evaluated. the cunningham ...201526276273
genetic diversity and population structure of synthesium pontoporiae (digenea, brachycladiidae) linked to its definitive host stocks, the endangered franciscana dolphin, pontoporia blainvillei (pontoporiidae) off the coast of brazil and argentina.pontoporia blainvillei (gervais and d'orbigny, 1844) is an endangered small cetacean endemic to south america with four franciscana management areas (fma) recognized as different population stocks. the role of the intestinal parasite synthesium pontoporiae (digenea: brachycladiidae) as a possible biological marker to differentiate p. blainvillei stocks was evaluated using nuclear and mitochondrial dna markers. internal transcribed sequence 1 and 2 (its1 and its2) regions of s. pontoporiae did no ...201526262593
can artemia hatching assay be a (sensitive) alternative tool to acute toxicity test?artemia sp. is extensively used in ecotoxicity testing, despite criticisms inherent to both acute and long-term tests. alternative endpoints and procedures should be considered to support the use of this biological model. the hatching process comprises several developmental steps and the cyst hatchability seems acceptable as endpoint criterion. in this study, we assessed the reliability of the hatching assay on a. franciscana by comparing with acute and long-term mortality tests, using two chemi ...201526245451
evidence of positive selection of aquaporins genes from pontoporia blainvillei during the evolutionary process of cetaceans.marine mammals are well adapted to their hyperosmotic environment. several morphological and physiological adaptations for water conservation and salt excretion are known to be present in cetaceans, being responsible for regulating salt balance. however, most previous studies have focused on the unique renal physiology of marine mammals, but the molecular bases of these mechanisms remain poorly explored. many genes have been identified to be involved in osmotic regulation, including the aquapori ...201526226365
population genetics of franciscana dolphins (pontoporia blainvillei): introducing a new population from the southern edge of their distribution.due to anthropogenic factors, the franciscana dolphin, pontoporia blainvillei, is the most threatened small cetacean on the atlantic coast of south america. four franciscana management areas have been proposed: espiritu santo to rio de janeiro (fma i), são paulo to santa catarina (fma ii), rio grande do sul to uruguay (fma iii), and argentina (fma iv). further genetic studies distinguished additional populations within these fmas. we analyzed the population structure, phylogeography, and demogra ...201526221960
comparing cestode infections and their consequences for host fitness in two sexual branchiopods: alien artemia franciscana and native a. salina from syntopic-populations.the american brine shrimp artemia franciscana is invasive in the mediterranean region where it has displaced native species (the sexual a. salina, and the clonal a. parthenogenetica) from many salt pond complexes. artemia populations are parasitized by numerous avian cestodes whose effects have been studied in native species. we present a study from the ebro delta salterns (ne spain), in a salt pond where both a. franciscana and native a. salina populations coexist, providing a unique opportunit ...201526157636
physiological strategies during animal diapause: lessons from brine shrimp and annual killifish.diapause is a programmed state of developmental arrest that typically occurs as part of the natural developmental progression of organisms that inhabit seasonal environments. the brine shrimp artemia franciscana and annual killifish austrofundulus limnaeus share strikingly similar life histories that include embryonic diapause as a means to synchronize the growth and reproduction phases of their life history to favorable environmental conditions. in both species, respiration rate is severely dep ...201526085666
helminth parasites of artemia franciscana (crustacea: branchiopoda) in the great salt lake, utah: first data from the native range of this invader of european wetlands.the present study is the first survey on the role of artemia franciscana kellogg as intermediate host of helminth parasites in its native geographical range in north america (previous studies have recorded nine cestode and one nematode species from this host in its invasive habitats in the western mediterranean). samples of artemia franciscana were collected from four sites in the great salt lake (gsl), utah, across several months (june-september 2009). a. franciscana serves as intermediate host ...201526040582
a review of the biology of australian halophilic anostracans (branchiopoda: anostraca).australia has two species of artemia: a. franciscana introduced to salt works and apparently not spreading, and parthenogenetic artemia presently spreading widely through southwestern australia. in addition, and unique to australia, there are 18 described species of parartemia in hypersaline lakes. all parartemia use a lock and key mechanism in amplexus and hence have distinctive antennal-head features in males and thoracic modifications in females. various factors, including climatic fluctuatio ...201425984503
enhancing the efficacy of electrolytic chlorination for ballast water treatment by adding carbon dioxide.we examined the synergistic effects of co2 injection on electro-chlorination in disinfection of plankton and bacteria in simulated ballast water. chlorination was performed at dosages of 4 and 6ppm with and without co2 injection on electro-chlorination. testing was performed in both seawater and brackish water quality as defined by imo g8 guidelines. co2 injection notably decreased from the control the number of artemia franciscana, a brine shrimp, surviving during a 5-day post-treatment incubat ...201525841887
effects of alginate on stability and ecotoxicity of nano-tio2 in artificial seawater.the large-scale use of titanium dioxide nanoparticles (nano-tio₂) in consumer and industrial applications raised environmental health and safety concerns. potentially impacted ecosystems include estuarine and coastal organisms. results from ecotoxicological studies with nano-tio₂ dispersed in salt exposure media are difficult to interpret due to fast flocculation and sedimentation phenomena affecting the dispersion stability. the goal of this study was to investigate the stabilisation effect of ...201525841066
toxicity of two imidazolium ionic liquids, [bmim][bf4] and [omim][bf4], to standard aquatic test organisms: role of acetone in the induced toxicity.the main goal of this study was to investigate the toxicity of the imidazolium-based ionic liquids (ils), [bmim][bf4] (1-butyl-3-methylimidazolium tetrafluoroborate) and [omim][bf4] (1-octyl-3-methylimidazolium tetrafluoroborate), in battery of standard aquatic toxicity test organisms. specifically, exposure of the algae scenedesmus rubescens, crustaceans thamnocephalus platyurus and artemia franciscana, rotifers brachionus calyciflorus and brachionus plicatilis and bivalve mytilus galloprovinci ...201525839183
renal lesions in cetaceans from brazil.this study reports the occurrence of renal lesions in cetaceans from the coast of brazil subjected to necropsy examination between 1996 and 2011. the animals (n = 192) were by-caught in fishing nets, were found dead on beaches or died despite attempted rehabilitation. kidney samples were evaluated grossly and microscopically and, depending on the histopathological findings, immunohistochemical and ultrastructural analyses were conducted. due to autolysis, a diagnosis was reached in only 128 anim ...201525824116
probing the protective mechanism of poly-ß-hydroxybutyrate against vibriosis by using gnotobiotic artemia franciscana and vibrio campbellii as host-pathogen model.the compound poly-ß-hydroxybutyrate (phb), a polymer of the short chain fatty acid ß-hydroxybutyrate, was shown to protect experimental animals against a variety of bacterial diseases, (including vibriosis in farmed aquatic animals), albeit through undefined mechanisms. here we aimed at unraveling the underlying mechanism behind the protective effect of phb against bacterial disease using gnotobiotically-cultured brine shrimp artemia franciscana and pathogenic vibrio campbellii as host-pathogen ...201525822312
molecular approaches for improving desiccation tolerance: insights from the brine shrimp artemia franciscana.we have evaluated the endogenous expression and molecular properties of selected group 3 lea proteins from artemia franciscana , and the capacity of selected groups 1 and 3 proteins transfected into various desiccation-sensitive cell lines to improve tolerance to drying. organisms inhabiting both aquatic and terrestrial ecosystems frequently are confronted with the problem of water loss for multiple reasons--exposure to hypersalinity, evaporative water loss, and restriction of intracellular wate ...201525809151
developmental critical windows and sensitive periods as three-dimensional constructs in time and space.a critical window (sensitive period) represents a period during development when an organism's phenotype is responsive to intrinsic or extrinsic (environmental) factors. such windows represent a form of developmental phenotypic plasticity and result from the interaction between genotype and environment. critical windows have typically been defined as comprising discrete periods in development with a distinct starting time and end time, as identified by experiments following an on and an off prot ...201525730265
aquatic pollution may favor the success of the invasive species a. franciscana.the genus artemia consists of several bisexual and parthenogenetic sibling species. one of them, a. franciscana, originally restricted to the new world, becomes invasive when introduced into ecosystems out of its natural range of distribution. invasiveness is anthropically favored by the use of cryptobiotic eggs in the aquaculture and pet trade. the mechanisms of out-competition of the autochthonous artemia by the invader are still poorly understood. ecological fitness may play a pivotal role, b ...201525723047
a comparison of the antimicrobial activity and toxicity of six combretum and two terminalia species from southern africa.plants of the family combretaceae are amongst the most widely used plants for traditional medicinal purposes in southern africa. in particular, many species of combretum and terminalia are used for their antibacterial, antifungal, antiprotozoal, antiviral, antidiarrhoeal, analgesic, antimalarial, antioxidant, anti-inflammatory and anticancer activities, yet their antimicrobial potential has not been rigorously studied and compared.201525709234
artemia franciscana as a vector for infectious myonecrosis virus (imnv) to litopenaeus vannamei juvenile.in 2004, the infectious myonecrosis virus (imnv) was recognized as the main cause of litopenaeusvannamei shrimp culture's drop in brazil. in health animal control programs, in order to reduce virus prevalence in production units it is necessary to screen live feed used. among live diets used in aquaculture, the brine shrimp artemia sp. is essential in crustacean larviculture and maturation. the aim of the present study was to investigate the susceptibility of artemiafranciscana to imnv through a ...201525676109
marine lactobacillus pentosus h16 protects artemia franciscana from vibrio alginolyticus pathogenic effects.vibrio alginolyticus is an opportunistic pathogen which may affect different aquatic organisms. the aim of this study was to assess the probiotic properties and the protective mode of action of lactobacillus pentosus h16 against v. alginolyticus 03/8525, through in vitro and in vivo studies using artemia franciscana (hereafter artemia). this strain showed antimicrobial activity against v. alginolyticus 03/8525 and aeromonas salmonicida subsp. salmonicida atcc33658 possibly related to lactobacill ...201525667335
detoxification and color removal of congo red by a novel dietzia sp. (dts26) - a microcosm approach.the present study deals with the decolorization and detoxification of congo red (cr) by a novel marine bacterium dietzia sp. (dts26) isolated from divar island, goa, india. the maximum decolorization of 94.5% (100 mg l(-1)) was observed under static condition within 30 h at ph 8 and temperature 32±2°c. bacterially treated samples could enhance the light intensity by 38% and the primary production levels 5 times higher than the untreated. the strain was also able to reduce cod by 86.4% within 30 ...201525600715
non-lethal heat shock induces hsp70 and hmgb1 protein production sequentially to protect artemia franciscana against vibrio campbellii. 201525463291
ecotoxicity of diethylene glycol and risk assessment for marine environment.diethylene glycol (deg) is a chemical compound used during offshore oil activities to prevent hydrate formation, and it may be released into the sea. a full ecotoxicological characterization is required according to european and italian regulations for chemical substances. we have evaluated long-term toxic effects of deg on indicator species of the marine environment as algae (phaeodactylum tricornutum), crustaceans (artemia franciscana), molluscs (tapes philippinarum) and fish (dicentrarchus la ...201525463226
sex-dependent transcriptome analysis and single nucleotide polymorphism (snp) discovery in the brine shrimp artemia franciscana.in order to enhance genomic resources for the brine shrimp artemia franciscana, rna-seq analysis was conducted for adult females and males. through de novo assembly, 36,896 high quality contigs were obtained, of which 13,749 sequences were annotated with arthropod sequences. just 4.5% matched against previously reported sequences for artemia spp. additionally, different transcriptional patterns between males and females were found, evidencing sex-related transcriptional responses. furthermore, 2 ...201425450167
a novel model of early development in the brine shrimp, artemia franciscana, and its use in assessing the effects of environmental variables on development, emergence, and hatching.the brine shrimp, artemia (crustacea, anostraca), is a zooplankton that is commonly used in both basic and applied research. unfortunately, artemia embryos are often cultured under conditions that alter early development, and reports based on these cultures oversimplify or fail to describe morphological phenotypes. this is due in part to the lack of a comprehensive developmental model that is applicable to observations of live specimens. the objective of this study was to build and test a descri ...201525427440
synthesis of maculalactone a and derivatives for environmental fate tracking studies.maculalactone a (1) constitutes a promising antifouling agent, inhibiting the formation of biofilms in marine and freshwater systems. in this study, we developed a new route, based on a late-stage formation of the butenolide core, leading to the total synthesis of maculalactone a (three steps, overall yield of 45%) and delivering material on a gram scale. in addition, analogues of the title compound were assayed concerning their biological activity, utilizing artemia franciscana and thamnocephal ...201525360848
supplemental food that supports both predator and pest: a risk for biological control?supplemental food sources to support natural enemies in crops are increasingly being tested and used. this is particularly interesting for generalist predators that can reproduce on these food sources. however, a potential risk for pest control could occur when herbivores also benefit from supplemental food sources. in order to optimize biological control, it may be important to select food sources that support predator populations more than herbivore populations. in this study we evaluated the ...201525349063
intracellular localization of group 3 lea proteins in embryos of artemia franciscana.late embryogenesis abundant (lea) proteins are accumulated by anhydrobiotic organisms in response to desiccation and improve survivorship during water stress. in this study we provide the first direct evidence for the subcellular localizations of afrlea2 and afrlea3m (and its subforms) in anhydrobiotic embryos of artemia franciscana. immunohistochemistry shows afrlea2 to reside in the cytoplasm and nucleus, and the four afrlea3m proteins to be localized to the mitochondrion. cellular locations a ...201425311474
how marine debris ingestion differs among megafauna species in a tropical coastal area.the marine debris ingested by megafauna species (trichiurus lepturus, chelonia mydas, pontoporia blainvillei, and sotalia guianensis) was recorded in a coastal area of southeastern brazil (21-23°s). marine debris was recorded in all species, mainly consisting of plastic material (flexible and hard plastics - clear, white, and colored- and nylon filaments). the 'pelagic predators' t. lepturus and s. guianesis showed the lowest percent frequencies of debris ingestion (0.7% and 1.3%, respectively), ...201425256299
group 3 late embryogenesis abundant proteins from embryos of artemia franciscana: structural properties and protective abilities during desiccation.group 3 late embryogenesis abundant (lea) proteins are highly hydrophilic, and their expression is associated with desiccation tolerance in both plants and animals. here we show that two lea proteins from embryos of artemia franciscana, afrlea2 and afrlea3m, are intrinsically disordered in solution but upon desiccation gain secondary structure, as measured by circular dichroism. trifluoroethanol and sodium dodecyl sulfate are both shown to induce α-helical structure in afrlea2 and afrlea3m. bioi ...201425244376
comparison of artemia-bacteria associations in brines, laboratory cultures and the gut environment: a study based on chilean hypersaline environments.the brine shrimp artemia (crustacea) and a diversity of halophilic microorganisms coexist in natural brines, salterns and laboratory cultures; part of such environmental microbial diversity is represented in the gut of artemia individuals. bacterial diversity in these environments was assessed by 16s rrna gene denaturing gradient gel electrophoresis (dgge) fingerprinting. eight natural locations in chile, where a. franciscana or a. persimilis occur, were sampled for analysis of free-living and g ...201525239570
bacillus sp. lt3 improves the survival of gnotobiotic brine shrimp (artemia franciscana) larvae challenged with vibrio campbellii by enhancing the innate immune response and by decreasing the activity of shrimp-associated vibrios.bacteria belonging to the genus bacillus are amongst the most intensively studied group of bacteria for use as probiotics in aquaculture. however, the exact mechanism of action of these bacteria is often not well described, and the microbiota that are naturally present in cultures of test organisms often compromise the interpretation of the results. the present study aimed to evaluate the putative probiotic effect of bacillus sp. lt3 in a model system with gnotobiotic brine shrimp artemia franci ...201425190276
a short-term laboratory and in situ sediment assay based on the postexposure feeding of the estuarine isopod cyathura carinata.in situ assays based on feeding depression have been proposed as sublethal assays able to assess immediate contaminant effects on key ecosystem functions, long before effects on life-history traits can be detected. the in situ peculiarities provide more realistic exposure scenarios than laboratory-controlled conditions, which is particularly relevant for estuarine ecosystems where environmental conditions are highly variable. in this context, we developed a short-term cost-effective in situ assa ...201425173058
a gnotobiotic model system: the case of artemia franciscana. 201325141743
effect of environmental factors on heritability and its biometrics of artemia franciscana vinh chau by mass selection of small sized cysts. 201325141698
surface area estimation of the gut segments of artemia franciscana nauplii fed with mnn9 yeast vs. wild type yeast. 201325141655
larval helminths in the invasive american brine shrimp artemia franciscana throughout its annual cycle.one of the best examples of rapid displacement of native species by an invader is the eradication of native artemia salina and a. parthenogenetica in the mediterranean by the introduced american a. franciscana. previous studies based on sampling from limited time periods suggest that the success of the american species as a competitor may be due partly to different parasite burden, since native artemia spp. have high cestode infection rates regulating their density. the aim of this study is to t ...201425119350
taxonomic utility of niche models in validating species concepts: a case study in anthophora (heliophila) (hymenoptera: apidae).taxonomy has far-reaching effects throughout biology, and incorrect taxonomy can be detrimental in many ways. polymorphic species complexes, many of which exist in the bee genus anthophora latreille, lend themselves to such difficulties. this study employs environmental niche mapping (enm) and traditional morphological analyses to investigate the validity of the subjective synonymy of anthophora (heliophila) curta provancher with the senior synonym a. squammulosa dours. eleven of fifty morpholog ...201425112259
ontogenetic development and sexual dimorphism of franciscana dolphin skull: a 3d geometric morphometric approach.the aim of this work was to study the postnatal ontogenetic development of pontoporia blainvillei skull, identifying major changes on shape, and relating them to relevant factors in the life history of the species. we analyzed a complete ontogenetic series (73♂, 83♀) with three-dimensional geometric morphometric techniques. immature dolphins showed a very well-developed braincase and a poorly developed rostrum, and the principal postnatal changes affected the rostrum and the temporal fossa, both ...201425052760
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