Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| virulence determines beneficial trade-offs in the response of virus-infected plants to drought via induction of salicylic acid. | it has been hypothesized that plants can get beneficial trade-offs from viral infections when grown under drought conditions. however, experimental support for a positive correlation between virus-induced drought tolerance and increased host fitness is scarce. we investigated whether increased virulence exhibited by the synergistic interaction involving potato virus x (pvx) and plum pox virus (ppv) improves tolerance to drought and host fitness in nicotiana benthamiana and arabidopsis thaliana. ... | 2017 | 28718885 |
| a novel system for predicting liver histopathology in patients with chronic hepatitis b. | there is currently a lack of reliable, reproducible, and easily applied methods for assessing changes in liver histology in patients in the gray zone phase of chronic hepatitis b (chb). therefore, we aimed to develop a novel predictive scoring system to detect significant liver histological changes in these patients.a total of 388 patients in the gray zone phase of chb who underwent liver biopsy were divided into a training group and a validation group, and their clinical and routinely available ... | 2017 | 28383410 |
| laboratory and molecular characterization of dengue virus during the 2014 outbreak in guangfo region, southern china. | non-specific symptoms and low viremia levels make early diagnosis of dengue virus (denv) infection challenging. this study aimed to 1) identify laboratory markers which can predict a positive laboratory diagnosis, and 2) perform a molecular characterization of denv from the 2014 guangdong epidemic. this retrospective study enrolled 1044 patients from the guangdong epidemic who had clinically suspected dengue. viral rna was detected by real-time rt-pcr and viral-specific ns1 antigen was detected ... | 2017 | 28367875 |
| periodic screening pelvic examination: evidence report and systematic review for the us preventive services task force. | recent changes in the periodicity of cervical cancer screening have led to questions about the role of screening pelvic examinations among asymptomatic women. | 2017 | 28267861 |
| non-invasive assessment of liver fibrosis in patients with hepatitis c: shear wave elastography and colour doppler velocity profile technique versus liver biopsy. | determination of the presence and degree of liver fibrosis is essential for the prognosis and treatment of patients with chronic hepatitis c. non-invasive methods of assessing fibrosis have been developed to reduce the need for biopsy. we determined the efficacy of shear wave elastography (swe) and colour doppler velocity as non-invasive methods for the assessment of liver fibrosis compared to liver biopsy among patients with chronic hepatitis c virus (hcv) infection. | 2017 | 28262531 |
| an evidence synthesis approach to estimating the proportion of influenza among influenza-like illness patients. | estimation of the national-level incidence of seasonal influenza is notoriously challenging. surveillance of influenza-like illness is carried out in many countries using a variety of data sources, and several methods have been developed to estimate influenza incidence. our aim was to obtain maximally informed estimates of the proportion of influenza-like illness that is true influenza using all available data. | 2017 | 28252453 |
| pathogen safety of a new intravenous immune globulin 10% liquid. | the manufacturing process of a new intravenous immune globulin (ivig) 10% liquid product incorporates two dedicated pathogen safety steps: solvent/detergent (s/d) treatment and nanofiltration (20 nm). ion-exchange chromatography (iec) during protein purification also contributes to pathogen safety. the ability of these three process steps to inactivate/remove viruses and prions was evaluated. | 2017 | 28236170 |
| experimental and computational surface hydrophobicity analysis of a non-enveloped virus and proteins. | the physical characteristics of viruses needs to be understood in order to manipulate the interaction of viruses with host cells, as well as to create specific molecular recognition techniques to detect, purify, and remove viruses. viruses are generally believed to be positively charged at physiological ph, but there are few other defining characteristics. here, we have experimentally and computationally demonstrated that a non-enveloped virus is more hydrophobic than a panel of model proteins. ... | 2017 | 28219841 |
| antiviral activity of a novel composition of peracetic acid disinfectant on parvoviruses. | porcine parvoviruses (ppv) are known to be particularly resistant to many disinfectants used to control other non-enveloped viruses. however, effective disinfectants used against ppv are harsh and corrosive to animal health facilities and the environment. we propose a noncorrosive "green" disinfectant that generates peracetic acid in-situ and is capable of inactivating ppv completely at a 1% concentration for a 10-minute contact time. | 2017 | 28154460 |
| performance of point-of-care urine test in diagnosing tuberculosis suspects with and without hiv infection in selected peripheral health settings of addis ababa, ethiopia. | there are few rapid point-of-care tests (poct) for tuberculosis (tb) for use in resource-constrained settings with high levels of human immunodeficiency virus (hiv). this hinders early tuberculosis (tb) treatment. this cross-sectional study evaluates the recently developed urine determine tuberculosis lipoarabinomannan (tb lam) antigen test. a total of 122 participants with signs and symptoms of tb, including 21 (17.1%) participants positive for hiv, were enrolled from september 2011 to march 20 ... | 2017 | 28137314 |
| predictors of extracapsular extension in hpv-associated oropharyngeal cancer treated surgically. | extracapsular extension (ece) in cervical metastatic lymph nodes remains an indication for adding chemotherapy for patients with oropharyngeal squamous cell carcinoma (opscc). the aim of this study is to identify specific imaging characteristics on computed tomography (ct) scan that are predictive of ece in order to better risk stratify patients preoperatively. | 2017 | 28109475 |
| predictors of liver histological changes and a sustained virological response to peginterferon among chronic hepatitis b e antigen-positive patients with normal or minimally elevated alanine aminotransferase levels. | a proportion of chronic hepatitis b patients with normal or only minimally elevated alanine aminotransferase (alt) levels display significant histologic changes and would benefit from antiviral therapy. we aim to evaluate the histologic abnormalities seen in these patients and then determine which of them would most likely respond to peginterferon therapy. one hundred and thirteen hepatitis b e antigen (hbeag)-positive patients with a normal or minimally elevated alt level and moderate-to-severe ... | 2017 | 28107601 |
| post-treatment 18f-fdg-pet/ct versus contrast-enhanced ct in patients with oropharyngeal squamous cell carcinoma: comparative effectiveness study. | to compare the accuracy of same-day therapy-assessment pet/computed tomography (pet/ct) and conventional contrast-enhanced computed tomography (cect) in patients with oropharyngeal squamous cell carcinoma (opscc). | 2017 | 28099264 |
| diagnosis and treatment of acute retinal necrosis: a report by the american academy of ophthalmology. | to evaluate the available evidence in peer-reviewed publications about the diagnosis and treatment of acute retinal necrosis (arn). | 2017 | 28094044 |
| evaluation of the point-of-care becton dickinson veritor™ rapid influenza diagnostic test in kenya, 2013-2014. | we evaluated the performance of the becton dickinson veritor™ system flu a + b rapid influenza diagnostic test (ridt) to detect influenza viruses in respiratory specimens from patients enrolled at five surveillance sites in kenya, a tropical country where influenza seasonality is variable. | 2017 | 28077093 |
| porcine parvovirus capsid protein expressed in escherichia coli self-assembles into virus-like particles with high immunogenicity in mice and guinea pigs. | porcine parvovirus (ppv) is a causative agent of reproductive failure in pregnant sows. classical inactivated vaccine is extensively used to control ppv infection, but problems concerning safety, such as incomplete inactivation may occur. in this study, a novel subunit vaccine against ppv based on virus-like particles (vlps) formed from the complete ppv vp2 protein expressed in a prokaryotic system with co-expressed chaperones is reported. the vlps have a similar size, shape, and hemagglutinatio ... | 2017 | 28063996 |
| do two screening tools for chikungunya virus infection that were developed among younger population work equally as well in patients aged over 65 years? | chikungunya is an endemo-epidemic infection, which is still considered as an emerging public health problem. the aim of this study was to evaluate in a 65+ population, the accuracy of two chikungunya screening scores that were developed in younger people. | 2017 | 28056031 |
| on-treatment quantitative hepatitis b e antigen predicted response to nucleos(t)ide analogues in chronic hepatitis b. | to investigate potential predictors for treatment response to nucleos(t)ide analogues (nas) in hepatitis b e antigen (hbeag)-positive chronic hepatitis b (chb) patients. | 2016 | 28008342 |
| new highly divergent plum pox virus isolates infecting sour cherry in russia. | unusual plum pox virus (ppv) isolates (named tat isolates) were discovered on sour cherry (prunus cerasus) in russia. they failed to be recognized by rt-pcr using commonly employed primers specific to the strains c or cr (the only ones that proved able to infect sour cherry) as well as to the strains m and w. some of them can be detected by rt-pcr using the ppv-d-specific primers p1/pd or by tas-elisa with the ppv-c-specific monoclonal antibody ac. phylogenetic analysis of the 3'-terminal genomi ... | 2017 | 28006670 |
| utility of enzyme-linked immunosorbent assays to test core antigen in the diagnosis and antiviral therapy management of hepatitis c virus infections. | in this study, we evaluate the performance of the enzyme-linked immunosorbent assays (elisas) for hcv ag detection in the diagnosis and antiviral therapy management of hcv infections. for the diagnosis of an active hcv infection, the limit of detection of hcv ag corresponding to hcv rna level was approximately 7300 iu/ml; the sensitivity, specificity, positive predictive value (ppv), and negative predictive value (npv) of hcv-ag were 88.96, 100, 100, and 91.33%, respectively. the pearson's corre ... | 2016 | 27958657 |
| clinical benefits of biochemical markers of fibrosis in egyptian children with chronic liver diseases. | the need for repetition of liver biopsy, especially in assessing the degree of fibrosis and follow-up of treatment protocols, justifies an intensive search for non-invasive alternatives. we attempted to investigate the clinical usefulness of serum fibrogenesis markers in pediatric chronic liver diseases. | 2010 | 27942306 |
| development of a novel rapid micro-neutralization elisa for the detection of neutralizing antibodies against chandipura virus. | chandipura virus (chpv) is a leading cause of acute encephalitis with high mortality in paediatric population in india. a micro-neutralization elisa (mn-elisa) assay was developed for the detection of neutralizing antibodies (nab) against chpv. this novel method gives read-out in the form of elisa optical density (od) values and has a shorter turn-around time (tat) as compared to the conventional cytopathic effect (cpe)-based neutralization assay (mn-cpe). the assay was developed using an indian ... | 2017 | 27856212 |
| logiq e9 shear wave elastrography for detection of liver fibrosis in patients with chronic hepatitis c virus. | shear wave elastography (swe) is an emerging technology that assesses hepatic fibrosis. the aim of our study was to determine the diagnostic accuracy of general electric (ge) healthcare's logiq e9 swe in patients with chronic hepatitis c virus using liver biopsy as the reference standard. | 2016 | 27812720 |
| molecular triage of premalignant lesions in liquid-based cervical cytology and circulating cell-free dna from urine, using a panel of methylated human papilloma virus and host genes. | clinically useful molecular tools to triage women for a biopsy upon referral to colposcopy are not available. we aimed to develop a molecular panel to detect cervical intraepithelial neoplasia (cin) grade 2 or higher lesions (cin2(+)) in women with abnormal cervical cytology and high-risk hpv (hpv(+)). we tested a biomarker panel in cervical epithelium dna obtained from 211 women evaluated in a cervical cancer clinic in chile from 2006 to 2008. results were verified in a prospective cohort of 10 ... | 2016 | 27671338 |
| epidemiological and immunopathological studies on porcine parvovirus infection in punjab. | the aim of this study was to get the first-hand knowledge about the seroprevalence of porcine parvovirus (ppv) in punjab and a diagnosis of ppv from abortion cases of swine using gross, histopathological, and immunohistopathological techniques to observe the tissue tropism of the virus strain. | 2016 | 27651669 |
| fighting sharka in peach: current limitations and future perspectives. | sharka, caused by plum pox virus (ppv), is by far the most important infectious disease of peach [p. persica (l.) batsch] and other prunus species. the progressive spread of the virus in many important growing areas throughout europe poses serious issues to the economic sustainability of stone fruit crops, peach in particular. the adoption of internationally agreed-upon rules for diagnostic tests, strain-specific monitoring schemes and spatial-temporal modeling of virus spread, are all essential ... | 2016 | 27625664 |
| hepatitis c virus core antigen assay: an alternative method for hepatitis c diagnosis. | background the study aimed to evaluate a fully automated chemiluminescent immunoassay and compared it with a quantitative rna assay and anti-hcv assay to verify the utility of this automated ag assay as an alternative method for hepatitis c diagnosis. methods a total of 229 serum samples previously tested for anti-hcv concentrations by the architect anti-hcv assay, were selected for hcv rna testing by real time rt-pcr kit (shanghai zj bio-tec co., ltd) and 125 specimens were tested for hcv ag by ... | 2017 | 27614354 |
| sugars and organic acids in plum fruit affected by plum pox virus. | plum pox virus (ppv) causes severe economic losses in stone fruit production, but little is known about its effect on plum fruit composition. in this study, the influence of ppv on sugars and organic acids was evaluated in a susceptible plum (prunus domestica l.) cultivar. | 2017 | 27614092 |
| a combination of different diagnostic tools allows identification of inactive hepatitis b virus carriers at a single time point evaluation. | serial evaluation of hepatitis b virus (hbv) dna and aminotransferase values is required for identification of inactive hbv carriers (ics). recently, hbv surface antigen quantification (qhbsag) and liver stiffness measurement (lsm) have been proposed as diagnostic tools in chronic hbv infection. the aim of this study was to evaluate the efficacy of hbv dna quantification, qhbsag and lsm in diagnosing ics at a single time point. | 2017 | 27606573 |
| diagnostic accuracy of apri and fib-4 for predicting hepatitis b virus-related liver fibrosis accompanied with hepatocellular carcinoma. | aspartate aminotransferase to platelet ratio index (apri) and the fibrosis index based on four factors (fib-4) are the two most focused non-invasive models to assess liver fibrosis. | 2016 | 27599803 |
| rapid and specific detection of porcine parvovirus by isothermal recombinase polymerase amplification assays. | porcine parvovirus (ppv) is a major cause of swine reproductive failure and reported in many countries worldwide. recombinase polymerase amplification (rpa) assays using a real-time fluorescent detection (ppv real-time rpa assay) and a lateral flow dipstick (ppv rpa lfd assay) were developed targeting ppv ns1 gene. the detection limit of ppv real-time rpa assay was 300 copies per reaction within 9 min at 38 °c, while the rpa lfd assay has a detection limit of 400 copies per reaction in less than ... | 2016 | 27593155 |
| an efficient viral vector for functional genomic studies of prunus fruit trees and its induced resistance to plum pox virus via silencing of a host factor gene. | rna silencing is a powerful technology for molecular characterization of gene functions in plants. a commonly used approach to the induction of rna silencing is through genetic transformation. a potent alternative is to use a modified viral vector for virus-induced gene silencing (vigs) to degrade rna molecules sharing similar nucleotide sequence. unfortunately, genomic studies in many allogamous woody perennials such as peach are severely hindered because they have a long juvenile period and ar ... | 2017 | 27565765 |
| new insights into the history of domesticated and wild apricots and its contribution to plum pox virus resistance. | studying domesticated species and their wild relatives allows understanding of the mechanisms of population divergence and adaptation, and identifying valuable genetic resources. apricot is an important fruit in the northern hemisphere, where it is threatened by the plum pox virus (ppv), causing the sharka disease. the histories of apricot domestication and of its resistance to sharka are however still poorly understood. we used 18 microsatellite markers to genotype a collection of 230 wild tree ... | 2016 | 27480465 |
| complete genome sequence of a novel potyvirus, callistephus mottle virus, identified in callistephus chinensis. | the complete genomic sequence of a novel putative member of the genus potyvirus was detected from callistephus chinensis (china aster) in south korea. the genomic rna consists of 9,859 nucleotides (excluding the 3' poly(a) tail) and contains the typical open reading frame of potyviruses, encoding a putative large polyprotein of 3,154 amino acids. the callistephus virus is most closely related to plum pox virus and members of the apvy subgroup which showed 50-52 % polyprotein amino acid sequence ... | 2016 | 27480416 |
| genotyping by sequencing (gbs) in apricots and genetic diversity assessment with gbs-derived single-nucleotide polymorphisms (snps). | genotyping by sequencing (gbs), which is a highly promising technique for molecular breeding, has been implemented in apricots, including turkish, european, and plum pox virus-resistant accessions. dna samples were digested with the apeki restriction enzyme to construct a genome-complexity-reduced 90-plex gbs library. after filtering the raw sequences, approximately 28 g of clean data were generated, and 17,842 high-quality single-nucleotide polymorphism (snp) loci were discovered. a total of 56 ... | 2016 | 27465591 |
| hepatitis e virus derived from different sources exhibits different behaviour in virus inactivation and/or removal studies with plasma derivatives. | hepatitis e virus (hev) causes viral hepatitis, and is considered a risk factor for blood products. although some hev inactivation/removal studies have been reported, detailed investigations of different manufacturing steps as heat treatment, partitioning during cold ethanol fractionation, low ph treatment, and virus filtration have yet to be reported for plasma-derived medicinal products. in this study, human serum- and swine faeces-derived hevs, with and without detergent treatment, were used. ... | 2016 | 27461242 |
| should viral load thresholds be lowered?: revisiting the who definition for virologic failure in patients on antiretroviral therapy in resource-limited settings. | the world health organization (who) guidelines on antiretroviral therapy (art) define treatment failure as 2 consecutive viral loads (vls) ≥1000 copies/ml. there is, however, little evidence supporting 1000 copies as an optimal threshold to define treatment failure. objective of this study was to assess the correlation of the who definition with the presence of drug-resistance mutations in patients who present with 2 consecutive unsuppressed vl in a resource-limited setting.in 10 nurse-led clini ... | 2016 | 27428189 |
| non-invasive fibrosis score for hepatitis delta. | identifying advanced fibrosis in chronic hepatitis delta patients and thus in need of urgent treatment is crucial. to avoid liver biopsy, non-invasive fibrosis scores may be helpful but have not been evaluated for chronic hepatitis delta yet. | 2017 | 27428078 |
| [immune response of recombinant pseudorabies virus rprv-vp2 expressing vp2 gene of porcine parvovirus in mice]. | in order to develop a combined live vaccine that will be used to prevent against porcine parvovirus (ppv) and pseudorabies virus (prv) infection, the vp2 gene of ppv was inserted into the transfer vector plasmid pg to produce the recombinant plasmid pgvp2. the plasmid pgvp2 and the genome of prv hb98 attenuated vaccine were transfected by using lipofectamine into swine testis cells for the homologous recombination. the recombinant virus rprv-vp2 was purified by selection of green fluorescence pl ... | 2016 | 27396164 |
| usefulness of bronchoscopic probe-based confocal laser endomicroscopy in the diagnosis of pneumocystis jirovecii pneumonia. | probe-based confocal laser endomicroscopy (pcle) is a novel technique that provides in vivo microscopic imaging of the distal lung. we hypothesized that the intra-alveolar exudates characterizing pneumocystis jirovecii pneumonia (pjp) can be identified by pcle in vivo and help in its diagnosis. | 2016 | 27362271 |
| validity of the coding for herpes simplex encephalitis in the danish national patient registry. | large health care databases are a valuable source of infectious disease epidemiology if diagnoses are valid. the aim of this study was to investigate the accuracy of the recorded diagnosis coding of herpes simplex encephalitis (hse) in the danish national patient registry (dnpr). | 2016 | 27330328 |
| serological tests do not predict residual fibrosis in hepatitis c cirrhotics with a sustained virological response to interferon. | liver biopsy (lb) has lost popularity to stage liver fibrosis in the era of highly effective anti-hepatitis c virus (hcv) therapy, yet diagnosis of persistent cirrhosis may have important implications following hcv eradication. as performance of serological non-invasive tests (nits) to predict residual fibrosis in non-viremic hcv patients is unknown, we investigated accuracy of nits to predict residual fibrosis in cirrhotics after a sustained virological response (svr) to interferon (ifn). | 2016 | 27304619 |
| plum pox virus capsid protein suppresses plant pathogen-associated molecular pattern (pamp)-triggered immunity. | the perception of pathogen-associated molecular patterns (pamps) by immune receptors launches defence mechanisms referred to as pamp-triggered immunity (pti). successful pathogens must suppress pti pathways via the action of effectors to efficiently colonize their hosts. so far, plant pti has been reported to be active against most classes of pathogens, except viruses, although this defence layer has been hypothesized recently as an active part of antiviral immunity which needs to be suppressed ... | 2016 | 27301551 |
| establishment of a nanoparticle-assisted rt-pcr assay to distinguish field strains and attenuated strains of porcine epidemic diarrhea virus. | porcine epidemic diarrhea virus (pedv) can cause serious disease and even death in neonatal piglets, resulting in serious damage to the swine industry worldwide. open reading frame 3 (orf3) is the only accessory gene in the pedv genome. previous studies have indicated that pedv vaccine strains have a partial deletion in orf3. in this study, a nanoparticle-assisted polymerase chain reaction (nanoparticle-assisted rt-pcr) assay targeting the orf3 of pedv was developed to distinguish pedv field str ... | 2016 | 27287433 |
| removal process of prion and parvovirus from human platelet lysates used as clinical-grade supplement for ex vivo cell expansion. | pooled human platelet lysate (hpl) is becoming the new gold standard as supplement for ex vivo cell culture for clinical protocols. however, the risk of pathogen contamination of hpl increases with the platelet pool size. we hypothesized that hollow fiber anion exchange membrane chromatography using qyuspeed d (qsd) could remove resistant and untested bloodborne pathogens, such as parvoviruses and prions, from hpl-supplemented growth media without substantially affecting their capacity to suppor ... | 2016 | 27260210 |
| utility of keywords from chest radiograph reports for pneumonia surveillance among hospitalized patients with influenza: the cdc influenza hospitalization surveillance network, 2008-2009. | transcripts from admission chest radiographs could aid in identification of pneumonia cases for public health surveillance. we assessed the reliability of radiographic data abstraction and performance of radiographic key terms to identify pneumonia in patients hospitalized with laboratory-confirmed influenza virus infection. | 2017 | 27252568 |
| evaluation of case definitions to detect respiratory syncytial virus infection in hospitalized children below 5 years in rural western kenya, 2009-2013. | in order to better understand respiratory syncytial virus (rsv) epidemiology and burden in tropical africa, optimal case definitions for detection of rsv cases need to be identified. | 2016 | 27207342 |
| multiplex reverse transcriptase-pcr for simultaneous detection of hepatitis b, c, and e virus. | the hepatitis b virus (hbv), hcv, and hev may occur as singly or concurrently in patients of different kind of liver disease. the rapid, reliable, and cost-effective screening of these pathogens is required for the large epidemiological studies. therefore, a study has been planned to develop a multiplex reverse transcriptase-pcr assay which can be used for the screening of maximum number of pathogens at a time. | 2016 | 27194894 |
| hcv ns3ag: a reliable and clinically useful predictor of antiviral outcomes in genotype 1b hepatitis c virus-infected patients. | since hepatitis c virus (hcv) non-structural 3 (ns3) protease inhibitor (pi) combined with pegylated interferon/ribavirin (pr) has been approved for chronic hcv genotype (gt) 1b infection, a reliable and clinically useful predictor combining with serum hcv rna to predict early virologic response, breakthrough, and relapse is important during hcv antiviral treatment. we evaluated the role of hcv ns3 antigen (hcv ns3ag) on the prediction of virologic response in patients with hcv gt1b during pr or ... | 2016 | 27173787 |
| quantitative and functional antibody responses to the 13-valent conjugate and/or 23-valent purified polysaccharide vaccine in aging hiv-infected adults. | the number of aging human immunodeficiency virus-infected (hiv+) individuals living in the united states has substantially grown over the past two decades. advanced age and hiv infection both increase susceptibility to streptococcus pneumoniae infection due to b cell dysfunction. the combined impact of these factors on pneumococcal vaccine responses remains unknown. | 2016 | 27158552 |
| neurosurgery and human immunodeficiency virus in the era of combination antiretroviral therapy: a review. | human immunodeficiency virus (hiv) is a global health problem. it renders the central nervous system susceptible to infectious and noninfectious diseases. hiv-positive individuals may present to neurosurgical services with brain lesions of unknown etiology. the differential diagnosis in these cases is broad, including opportunistic infections and malignancies, and investigation should be tailored accordingly. opportunistic infections of the central nervous system can be complicated by hydrocepha ... | 2017 | 27081898 |
| diagnostic performance of influenza viruses and rsv rapid antigen detection tests in children in tertiary care. | rapid antigen detection tests (radts) are increasingly used to detect influenza viruses and respiratory syncytial virus (rsv). however, their sensitivity and specificity are a matter of debate, challenging their clinical usefulness. | 2016 | 27045454 |
| diagnostic potential of monoclonal antibodies against the capsid protein of chikungunya virus for detection of recent infection. | chikungunya fever is self-limiting. however, neurological and hemorrhagic complications have been seen in recent outbreaks. the clinical manifestations of this disease are similar to those of dengue virus infection, indicating the need for differential diagnosis in areas such as india, which are endemic for both viruses. the aim of the present study was to develop monoclonal antibodies (mabs) against chikungunya virus (chikv) and assess their use in mab-based igm capture elisa (mac elisa). the e ... | 2016 | 27016930 |
| role of signal-to-cut-off ratios of anti-hepatitis c virus antibody by enzyme immunoassays along with id-nat for screening of whole blood donors in india. | the use of elevated signal-to-cut off ratios (s/co) as an alternate to further supplemental testing (i.e., riba) has been included in the guidelines provided by the centres for disease control and prevention for hcv diagnostic purposes since 2003. with availability of screening by nat and non availability of riba, further confirmation of hcv infection has been possible at the molecular level (rna). | 2017 | 27011676 |
| provocation proven drug allergy in thai children with adverse drug reactions. | adverse drug reactions (adrs) are a common healthcare problem. the drug provocation test (dpt) is a gold standard for adr diagnosis. | 2016 | 26994627 |
| hepatitis c viral kinetic changes in a retrospective cohort study of chronic hepatitis c virus egyptian patients on pegylated interferon and ribavirin therapy. | the aim of this study was to determine the relative importance of the kinetics of antiviral response compared to baseline host and virological factors for predicting treatment outcome. a retrospective analysis of 285 chronic hepatitis c virus (hcv) patients, encompassing genotypes 4 treated with peginterferon alpha-2a and ribavirin, was performed. baseline characteristics were compared across hcv genotypes and pretreatment factors associated with rapid virological response (rvr) were identified. ... | 2016 | 26982165 |
| plum pox virus 6k1 protein is required for viral replication and targets the viral replication complex at the early stage of infection. | the potyviral rna genome encodes two polyproteins that are proteolytically processed by three viral protease domains into 11 mature proteins. extensive molecular studies have identified functions for the majority of the viral proteins. for example, 6k2, one of the two smallest potyviral proteins, is an integral membrane protein and induces the endoplasmic reticulum (er)-originated replication vesicles that target the chloroplast for robust viral replication. however, the functional role of 6k1, ... | 2016 | 26962227 |
| incidence of hospitalization for respiratory syncytial virus infection amongst children in ontario, canada: a population-based study using validated health administrative data. | rsv is a common illness among young children that causes significant morbidity and health care costs. | 2016 | 26958849 |
| a pilot study to compare the detection of hpv-16 biomarkers in salivary oral rinses with tumour p16(ink4a) expression in head and neck squamous cell carcinoma patients. | human papilloma virus-16 (hpv-16) infection is a major risk factor for a subset of head and neck squamous cell carcinoma (hnscc), in particular oropharyngeal squamous cell carcinoma (opscc). current techniques for assessing the hpv-16 status in hnscc include the detection of hpv-16 dna and p16(ink4a) expression in tumor tissues. when tumors originate from hidden anatomical sites, this method can be challenging. a non-invasive and cost-effective alternative to biopsy is therefore desirable for hp ... | 2016 | 26940728 |
| an inactivated whole-virus porcine parvovirus vaccine protects pigs against disease but does not prevent virus shedding even after homologous virus challenge. | inactivated whole-virus vaccines against porcine parvovirus (ppv) can prevent disease but not infection and virus shedding after heterologous virus challenge. here, we showed that the same is true for a homologous challenge. pregnant sows were vaccinated with an experimental inactivated vaccine based on ppv strain 27a. they were challenged on day 40 of gestation with the virulent porcine parvovirus ppv-27a from which the vaccine was prepared (homologous challenge). on day 90 of gestation, the fe ... | 2016 | 26939976 |
| comparison of directigen flu a+b with real time pcr in the diagnosis of influenza. | early diagnosis and treatment of patients with influenza is the reason why physicians need rapid high-sensitivity influenza diagnostic tests that require no complex lab equipment and can be performed and interpreted within 15 min. the aim of this study was to compare the rapid directigen flu a+b test with real time pcr for detection of influenza viruses in the republic of macedonia. | 2016 | 26933779 |
| ziehl-neelsen microscopy in the diagnosis of tuberculosis in settings of high human immunodeficiency virus prevalence. | to determine the accuracy of ziehl-neelsen microscopy in the diagnosis of tb in setings of high hiv prevalence. | 2012 | 26852457 |
| porcine parvovirus as a contaminant in cell cultures and laboratory supplies. | although ppv has been described as a cellular contaminant, few recent studies about the presence of this virus in cell cultures, serum, and trypsin were found in the literature. the purpose of this study was to detect the presence of porcine parvovirus (ppv) by polymerase chain reaction (pcr) in cell cultures, serum, and trypsin used in official public laboratories of educational institutes and research centers. we tested samples of cell cultures (88), batches of trypsin (10), and fetal bovine s ... | 2016 | 26811218 |
| improving the performance of reflex human papilloma virus (hpv) testing in triaging women with atypical squamous cells of undetermined significance (ascus): a restrospective study in a tertiary hospital in united arab emirates (uae). | cervical cancer is the second commonest cancer in women worldwide. infection with oncogenic types of human papillomavirus (hpv) is the most important risk factor for developing cervical cancer. reflex high risk hpv (hr-hpv) testing is of significant value in the assessment of papanicolaou (pap) smear results where ascus are identified. | 2016 | 26747717 |
| a rapid protocol of crude rna/dna extraction for rt-qpcr detection and quantification of 'candidatus phytoplasma prunorum'. | many efforts have been made to develop a rapid and sensitive method for phytoplasma and virus detection. taking our cue from previous works, different rapid sample preparation methods have been tested and applied to candidatus phytoplasma prunorum ('ca. p. prunorum') detection by rt-qpcr. a duplex rt-qpcr has been optimized using the crude sap as a template to simultaneously amplify a fragment of 16s rrna of the pathogen and 18s rrna of the host plant. the specific plant 18s rrna internal contro ... | 2016 | 26742106 |
| alterations in serotype-specific b cell responses to the 13-valent pneumococcal conjugate vaccine in aging hiv-infected adults. | advanced age and human immunodeficiency virus (hiv) infection are associated with increased pneumococcal disease risk. the impact of these factors on cellular responses to vaccination is unknown. | 2016 | 26707220 |
| simultaneous typing of seven porcine pathogens by multiplex pcr with a gexp analyser. | a novel high-throughput method was developed for simultaneous detection and differentiation of seven porcine pathogens by multiplex pcr based on a genomelab gene expression profiler (gexp) analyser. the pathogens included in this study were pseudorabies virus (prv), classical swine fever virus (csfv), african swine fever virus (asfv), porcine reproductive and respiratory syndrome virus (prrsv), porcine parvovirus (ppv), porcine circovirus type 2 (pcv-2) and japanese encephalitis virus (jev). sev ... | 2016 | 26706731 |
| immunogenicity of adenovirus-derived porcine parvovirus-like particles displaying b and t cell epitopes of foot-and-mouth disease. | virus-like particles (vlps) vaccines combine many of the advantages of whole-virus vaccines and recombinant subunit vaccines, integrating key features that underlay their immunogenicity, safety and protective potential. we have hypothesized here the effective insertion of the vp1 epitopes (three amino acid residues 21-40, 141-160 and 200-213 in vp1, designated vpe) of foot-and-mouth disease (fmdv) within the external loops of ppv vp2 could be carried out without altering assembly based on struct ... | 2016 | 26685093 |
| genome sequence analysis of new isolates of the winona strain of plum pox virus and the first definitive evidence of intrastrain recombination events. | plum pox virus (ppv) is genetically diverse with nine different strains identified. mutations, indel events, and interstrain recombination events are known to contribute to the genetic diversity of ppv. this is the first report of intrastrain recombination events that contribute to ppv's genetic diversity. fourteen isolates of the ppv strain winona (w) were analyzed including nine new strain w isolates sequenced completely in this study. isolates of other strains of ppv with more than one isolat ... | 2016 | 26667187 |
| ppv susceptibility of commonly used peach rootstock-scion combinations. | sharka disease is one of the most devastating plant epidemics of prunus species, caused by plum pox virus (ppv). the viral infection affects the fruits by weight-loss and degradation of quality properties. breeding of resistant rootstocks and cultivars is one of the most effective disease control methods. ppv determines the peach production all over the world. on the world's fruit production list peach is in the sixth, in the mediterranean region in the fourth place. in this study new data were ... | 2015 | 26666193 |
| differentially expressed genes in healthy and plum pox virus-infected nicotiana benthamiana plants. | viruses use both material and energy sources of their hosts and redirect the production of disposable compounds in order to make viral replication more efficient. metabolism of infected organisms is modified by these enhanced requirements as well by their own defense response. resulting complex story consists of many regulation events on various gene expression levels. elucidating these processes may contribute to the knowledge on virus-host interactions and to evolving new antiviral strategies. ... | 2015 | 26666187 |
| gene expression analysis of plum pox virus (sharka) susceptibility/resistance in apricot (prunus armeniaca l.). | rna-seq has proven to be a very powerful tool in the analysis of the plum pox virus (ppv, sharka disease)/prunus interaction. this technique is an important complementary tool to other means of studying genomics. in this work an analysis of gene expression of resistance/susceptibility to ppv in apricot is performed. rna-seq has been applied to analyse the gene expression changes induced by ppv infection in leaves from two full-sib apricot genotypes, "rojo pasión" and "z506-7", resistant and susc ... | 2015 | 26658051 |
| phase ii study of personalized peptide vaccination with both a hepatitis c virus-derived peptide and peptides from tumor-associated antigens for the treatment of hcv-positive advanced hepatocellular carcinoma patients. | objective. to evaluate safety and immune responses of personalized peptide vaccination (ppv) for hepatitis c virus- (hcv-) positive advanced hepatocellular carcinoma (hcc). patients and methods. patients diagnosed with hcv-positive advanced hcc were eligible for this study. a maximum of four hla-matched peptides were selected based on the preexisting igg responses specific to 32 different peptides, which consisted of a single hcv-derived peptide at core protein positions 35-44 (c-35) and 31 pept ... | 2015 | 26539554 |
| occurrence and characterization of plum pox virus strain d isolates from european russia and crimea. | numerous plum pox virus (ppv) strain d isolates have been found in geographically distant regions of european russia and the crimean peninsula on different stone fruit hosts. phylogenetic analysis of their partial and complete genomes suggests multiple introductions of ppv-d into russia. distinct natural isolates from prunus tomentosa were found to bear unique amino acid substitutions in the n-terminus of the coat protein (cp) that may contribute to the adaptation of ppv-d to this host. serologi ... | 2016 | 26530833 |
| assessing the mismatch between incubation and latent periods for vector-borne diseases: the case of sharka. | the relative durations of the incubation period (the time between inoculation and symptom expression) and of the latent period (the time between inoculation and infectiousness of the host) are poorly documented for plant diseases. however, the extent of asynchrony between the ends of these two periods (i.e., their mismatch) can be a key determinant of the epidemic dynamics for many diseases and consequently it is of primary interest in the design of disease management strategies. in order to ass ... | 2015 | 26512749 |
| first identification of porcine parvovirus 6 in north america by viral metagenomic sequencing of serum from pigs infected with porcine reproductive and respiratory syndrome virus. | currently, eight species in four genera of parvovirus have been described that infect swine. these include ungulate protoparvovirus 1 (classical porcine parvovirus, ppv), ungulate tetraparvovirus 2 (ppv3), ungulate tetraparvovirus 3 (which includes ppv2, porcine hokovirus, porcine partetravirus and porcine parv4), ungulate copiparvovirus 2 (which includes ppv4 and ppv5), ungulate bocaparvovirus 2 (which includes porcine bocavirus 1, 2 and 6), ungulate bocaparvovirus 3 (porcine bocavirus 5), ungu ... | 2015 | 26475593 |
| molecular epidemiology and evolution of porcine parvoviruses. | porcine parvovirus (ppv), recently named ungulate protoparvovirus 1, is considered to be one of the most important causes of reproductive failure in swine. fetal death, mummification, stillbirths and delayed return to estrus are predominant clinical signs commonly associated with ppv infection in a herd. it has recently been shown that certain parvoviruses exhibit a nucleotide substitution rate close to that commonly determined for rna viruses. however, the ppv vaccines broadly used in the last ... | 2015 | 26453771 |
| aphid transmission of the ontario isolate of plum pox virus. | utilization of timed virus acquisition access probes in studies of plum pox virus (ppv) transmission by aphids demonstrated that endemic species transmitted the virus readily from plum, prunus domestica (l.) batsch; peach, p. persica (l.); or dwarf flowering almond, p. glandulosa thunberg., to peach seedlings. the green peach aphid, myzus persicae (sulzer), was shown to be the most efficient vector. acquisition of virus by green peach aphids from infected peach leaves resulted in 18-28% infected ... | 2015 | 26453705 |
| evaluation of alpha-fetoprotein as a screening marker for hepatocellular carcinoma in hepatitis prevalent areas. | the objective of this study was to establish modified cutoff values of serum alpha-fetoprotein (afp) according to hepatitis status. while afp is used as a serum marker in the diagnosis or monitoring of hepatocellular carcinoma (hcc), its use as a screening method to the general population is controversial. we evaluated its screening performance in a hepatitis prevalent east asian population, and suggest different cutoff values according to the individual's hepatitis status. we evaluated the perf ... | 2015 | 26436360 |
| shedding patterns of endemic eurasian wild boar (sus scrofa) pathogens. | the eurasianwild boar has experienced aworldwide demographic explosion that increases awareness on shared pathogens. however, shedding routes of relevant wild boar pathogens are unknown. previous observations on sex- and age-related differences in aujeszky's disease virus (adv) exposure led us to hypothesize that shedding patterns of endemicwild boar pathogens may be influenced by individual traits.we investigated shedding routes of adv, porcine parvovirus (ppv), porcine circovirus type 2 (pcv2) ... | 2015 | 26412545 |
| a prospective study on the predictive value of plasma bk virus-dna load for hemorrhagic cystitis in pediatric patients after stem cell transplantation. | in hematopoietic stem cell transplantation (hsct), late hemorrhagic cystitis (hc) has been associated with bk virus (bkv) infection. we assessed the value of plasma bkv load in predicting hc. | 2015 | 26407413 |
| the pathogenic role of torque teno sus virus 1 and 2 and their correlations with various viral pathogens and host immunocytes in wasting pigs. | the pathogenic role of torque teno sus virus (ttsuv) in swine is controversial among different studies. the present study intended to evaluate the potential pathogenicity of ttsuv based on its correlations with the histopathological changes, various common concurrently infected viral pathogens including porcine circovirus type 2 (pcv2), porcine reproductive and respiratory syndrome virus (prrsv), and porcine parvovirus (ppv), as well as changes in the distribution and population of host immunocy ... | 2015 | 26390821 |
| rapid prediction of treatment futility of boceprevir with peginterferon-ribavirin for taiwanese treatment experienced hepatitis c virus genotype 1-infected patients. | the efficacy and safety of the boceprevir (boc)-containing triple therapy in taiwanese treatment-experienced patients remains elusive. after 4 weeks of peginterferon/ribavirin lead-in therapy, patients with cirrhosis or previous null-response received triple therapy for 44 weeks; whereas others received 32 weeks of triple therapy followed by 12 weeks of peginterferon/ribavirin therapy. patients with hcv rna > 100 iu/ml at week 12 or with detectable hcv rna at week 24 of treatment were viewed as ... | 2015 | 26368130 |
| genome-wide association links candidate genes to resistance to plum pox virus in apricot (prunus armeniaca). | in fruit tree species, many important traits have been characterized genetically by using single-family descent mapping in progenies segregating for the traits. however, most mapped loci have not been sufficiently resolved to the individual genes due to insufficient progeny sizes for high resolution mapping and the previous lack of whole-genome sequence resources of the study species. to address this problem for plum pox virus (ppv) candidate resistance gene identification in prunus species, we ... | 2016 | 26356603 |
| combining billing codes, clinical notes, and medications from electronic health records provides superior phenotyping performance. | to evaluate the phenotyping performance of three major electronic health record (ehr) components: international classification of disease (icd) diagnosis codes, primary notes, and specific medications. | 2016 | 26338219 |
| poor positive predictive value of influenza-like illness criteria in adult transplant patients: a case for multiplex respiratory virus pcr testing. | respiratory viral infections (rvis) are a significant cause of morbidity and mortality among transplant patients. the cdc's influenza-like illness (ili) criteria (fever ≥100°f with cough and/or sore throat) are a screening tool for influenza with unknown applicability to the transplant population. | 2015 | 26338182 |
| phylogenetic analysis of eight sudanese camel contagious ecthyma viruses based on b2l gene sequence. | camel contagious ecthyma (cce) is an important viral disease of camelids caused by a poxvirus of the genus parapoxvirus (ppv) of the family poxviridae. the disease has been reported in west and east of the sudan causing economical losses. however, the ppvs that cause the disease in camels of the sudan have not yet subjected to genetic characterization. at present, the ppv that cause cce cannot be properly classified because only few isolates that have been genetically analyzed. | 2015 | 26260127 |
| simultaneous detection of eight swine reproductive and respiratory pathogens using a novel gexp analyser-based multiplex pcr assay. | a new high-throughput genomelab gene expression profiler (gexp) analyser-based multiplex pcr assay was developed for the detection of eight reproductive and respiratory pathogens in swine. the reproductive and respiratory pathogens include north american porcine reproductive and respiratory syndrome virus (prrsv-na), classical swine fever virus (csfv), porcine circovirus 2 (pcv-2), swine influenza virus (siv) (including h1 and h3 subtypes), porcine parvovirus (ppv), pseudorabies virus (prv) and ... | 2015 | 26259690 |
| the clinical significance of hcv core antigen detection during telaprevir/peg-interferon/ribavirin therapy in patients with hcv 1 genotype infection. | direct-acting antiviral drugs (daa) regimen improve the svr rate. however, adverse effects often lead to therapy interruption. this underlines the importance to find some predictive parameters of response in order to consider the possibility of a shorter time of antiviral treatment in the appearance of adverse effects without affecting the success of the therapy. | 2015 | 26209382 |
| nucleus accumbens-associated protein 1 expression has potential as a marker for distinguishing oral epithelial dysplasia and squamous cell carcinoma. | oral epithelial dysplasia (oed) and carcinoma in situ (cis) are defined by dysplastic cells in the epithelium. over a third of oral squamous cell carcinoma (oscc) patients present with associated oed. however, accurate histopathological diagnosis of such lesions is difficult. nucleus accumbens-associated protein 1 (nac1) is a member of the pox virus and zinc finger/bric-a-brac tramtrack broad complex family of proteins, and is overexpressed in oscc. this study aimed to determine whether nac1 has ... | 2015 | 26172271 |
| persistent corneal epithelial defect after pars plana vitrectomy. | to investigate the incidence, risk factors, and clinical course of persistent corneal epithelial defects (pced) after pars plana vitrectomy (ppv). | 2016 | 26166798 |
| assorted processing of synthetic trans-acting sirnas and its activity in antiviral resistance. | the use of syn-tasirnas has been proposed as an rna interference technique alternative to those previously described: hairpin based, virus induced gene silencing or artificial mirnas. in this study we engineered the tas1c locus to impair plum pox virus (ppv) infection by replacing the five native sirnas with two 210-bp fragments from the cp and the 3´ncr regions of the ppv genome. deep sequencing analysis of the small rna species produced by both constructs in planta has shown that phased proces ... | 2015 | 26147769 |
| combined serum biomarkers in non-invasive diagnosis of non-alcoholic steatohepatitis. | non-alcoholic steatoheaptitis (nash), the critical stage of non-alcoholic fatty liver disease (nafld), is of chronic progression and can develop cirrhosis even hepatocellular carcinoma (hcc). however, non-invasive biomarkers for nash diagnosis remain poorly applied in clinical practice. our study aims at testing the accuracy of the combination of cytokeratin-18 m30 fragment (ck-18-m30), fibroblast growth factor 21 (fgf-21), interleukin 1 receptor antagonist (il-1ra), pigment epithelium-derived f ... | 2015 | 26121037 |
| self-reported hepatitis a vaccination as a predictor of hepatitis a virus antibody protection in u.s. adults: national health and nutrition examination survey 2007-2012. | to estimate the predictive value of self-reported hepatitis a vaccine (hepa) receipt for the presence of hepatitis a virus (hav) antibody (anti-hav) from either past infection or vaccination, as an indicator of hav protection. | 2015 | 26116252 |
| allelic variation at the rpv1 locus controls partial resistance to plum pox virus infection in arabidopsis thaliana. | sharka is caused by plum pox virus (ppv) in stone fruit trees. in orchards, the virus is transmitted by aphids and by grafting. in arabidopsis, ppv is transferred by mechanical inoculation, by biolistics and by agroinoculation with infectious cdna clones. partial resistance to ppv has been observed in the cvi-1 and col-0 arabidopsis accessions and is characterized by a tendency to escape systemic infection. indeed, only one third of the plants are infected following inoculation, in comparison wi ... | 2015 | 26109391 |
| biotechnological strategies and tools for plum pox virus resistance: trans-, intra-, cis-genesis, and beyond. | plum pox virus (ppv) is the etiological agent of sharka, the most devastating and economically important viral disease affecting prunus species. it is widespread in most stone fruits producing countries even though eradication and quarantine programs are in place. the development of resistant cultivars and rootstocks remains the most ecologically and economically suitable approach to achieve long-term control of sharka disease. however, the few ppv resistance genetic resources found in prunus ge ... | 2015 | 26106397 |
| parallel enzyme-linked immunosorbent assay screening for human immunodeficiency virus among blood donors in five chinese blood centres: a retrospective analysis. | to evaluate the strategy of parallel screening with different enzyme-linked immunosorbent assays (elisa) among chinese blood donors. | 2015 | 26104756 |
| viral inactivation of human osteochondral grafts with methylene blue and light. | cartilage injury is one of the most common disorders of synovial joints. fresh osteochondral allografts are becoming a standard treatment; however, they are supply constrained with a potential risk of disease transmission. there are no known virucidal processes available for osteochondral allografts and most methods presently available are detrimental to cartilage. methylene blue light treatment has been shown to be successful in the literature for viral inactivation of fresh frozen plasma. the ... | 0 | 26069682 |
| sharka epidemiology and worldwide management strategies: learning lessons to optimize disease control in perennial plants. | many plant epidemics that cause major economic losses cannot be controlled with pesticides. among them, sharka epidemics severely affect prunus trees worldwide. its causal agent, plum pox virus (ppv; genus potyvirus), has been classified as a quarantine pathogen in numerous countries. as a result, various management strategies have been implemented in different regions of the world, depending on the epidemiological context and on the objective (i.e., eradication, suppression, containment, or res ... | 2015 | 26047559 |
| native electrophoresis and western blot analysis (neweb): methods and applications. | native electrophoresis and western blot analysis (neweb) has been developed for the study of plant virus characteristics, among others, virus particle-protein interactions, electrophorotype formation, and strain separation. the method is based on the property of electrophoretic mobility of virus particles (vp) and proteins and combines the analytical capacity of electrophoresis with the specificity of western blot. one of its advantages is that it deals with entire vp that can be studied in caus ... | 2015 | 26044016 |